SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF3435 FitnessBrowser__WCS417:GFF3435 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q88H32 Ornithine cyclodeaminase; OCD; EC 4.3.1.12 from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) (see paper)
62% identity, 96% coverage: 1:335/348 of query aligns to 1:336/350 of Q88H32

query
sites
Q88H32

M

M

T

T

R

Y

Y

F

I

I

D

D

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V

N

P

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T

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M

C

S

Y

D

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G

E

E

M

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A

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D

D

Y

F

L

K

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W

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Q

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A

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E

D

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K

C

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A

A

R
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R

L

V

A

A

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H

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S

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E

D

V

G

G

V

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I

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E

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D

A

K

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Y

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K

Y

Y

V

V

N

N

G

G

H

H

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A

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N

T

T

L

A

A

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G

N

M

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T

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V

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M

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A

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L

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A

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D

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V

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Y

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K

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D

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A

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D

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A

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Y

A

A

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T

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T

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E

D

M

M

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L

E

E

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P

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G

M

M

H

H

L

L

N

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A

A

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V

G
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G

D
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D

C

C

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K

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T

E

E

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L

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G

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H

R45 (= R45) binding
K69 (= K69) binding
T84 (= T84) binding
R112 (= R112) binding ; binding
AQ 139:140 (≠ SQ 139:140) binding
D161 (= D161) binding
T202 (= T202) binding
VGGD 225:228 (= VGGD 225:228) binding
D228 (= D228) binding
K232 (= K232) binding
S293 (= S293) binding
V294 (= V294) binding
K331 (= K330) binding

1x7dA Crystal structure analysis of ornithine cyclodeaminase complexed with NAD and ornithine to 1.6 angstroms (see paper)
62% identity, 96% coverage: 2:335/348 of query aligns to 1:335/340 of 1x7dA

query
sites
1x7dA

T

T

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Y

Y

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Y

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K

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W

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Q

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A

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F

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D

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A

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x
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L

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K

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K

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G

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D

I

I

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T

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V

T
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T

A
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A

D

D

K

K

A

A

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Y

A

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T

T

I
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L

I

T

T

D

P

E

D

M

M

I

L

E

E

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P

G

G

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M

H

H

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L

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N

A

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V

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G

G

D
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D

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C

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P

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G

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K

T

T

E

E

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L

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I

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V

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A

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Q

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E

G

G

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S

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Q

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V

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L

V

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F

D

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S

V
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G
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G

F

F

A

A

I

L

E

E

D

D

Y

Y

S

T

A

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K

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H

active site: E55 (= E56), D227 (= D228)
binding nicotinamide-adenine-dinucleotide: T83 (= T84), V84 (= V85), T108 (= T109), R111 (= R112), T112 (= T113), G135 (= G136), G137 (= G138), A138 (≠ S139), Q139 (= Q140), Y159 (≠ F160), D160 (= D161), T161 (≠ I162), D162 (= D163), V200 (= V201), T201 (= T202), A202 (= A203), I209 (= I210), V224 (= V225), G225 (= G226), D227 (= D228), K231 (= K232), S292 (= S293), V293 (= V294), G294 (= G295)
binding L-ornithine: R44 (= R45), V53 (= V54), E55 (= E56), M57 (= M58), K68 (= K69), V70 (= V71), N71 (= N72), G72 (= G73), R111 (= R112), D227 (= D228), V293 (= V294), G294 (= G295)

1u7hA Structure and a proposed mechanism for ornithine cyclodeaminase from pseudomonas putida (see paper)
62% identity, 96% coverage: 2:335/348 of query aligns to 1:335/341 of 1u7hA

query
sites
1u7hA

T

T

R

Y

Y

F

I

I

D

D

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V

N

P

D

T

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M

C

S

Y

D

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Q

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E

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Y

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A

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K

Y

Y

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G

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H

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K

A

N

N

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L

A

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M

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L

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D

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A

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G

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x
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A

A

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G

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A
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D

D

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K

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A

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L

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A

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E

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V

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F

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D

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G

F

F

A

A

I

L

E

E

D

D

Y

Y

S

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A

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L

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Y

Y

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Q

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E

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M

G

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K

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D

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L

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E

-

D

D

D

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P

K

K

D

D

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L

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F

A

S

R

H

active site: E55 (= E56), D227 (= D228)
binding nicotinamide-adenine-dinucleotide: T83 (= T84), V84 (= V85), R111 (= R112), T112 (= T113), G135 (= G136), G137 (= G138), A138 (≠ S139), Q139 (= Q140), Y159 (≠ F160), D160 (= D161), T161 (≠ I162), D162 (= D163), V200 (= V201), T201 (= T202), A202 (= A203), I209 (= I210), V224 (= V225), G225 (= G226), D227 (= D228), K231 (= K232), S292 (= S293), V293 (= V294), G294 (= G295)

1omoA Alanine dehydrogenase dimer w/bound NAD (archaeal) (see paper)
34% identity, 78% coverage: 53:322/348 of query aligns to 49:319/320 of 1omoA

query
sites
1omoA

G

G

V

D

I

L

E
x
R

L

A

M

M

P

P

V

A

S

H

D

L

A

M

S

G

L

Y

Y

A

A

G

F

L

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K

Y

W

V

V

N

N

G

S

H

H

P

P

K

G

N

N

T

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L

D

A

K

G

G

M

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L

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T

T

V

V

M

M

A

A

F

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G

M

A

I

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N

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E

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T

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L

A

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A

M

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D

M

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T

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T

A

G

A

A

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G

A

G

L

I

V

A

A

A

R

K

Y

Y

L

L

A

A

R

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D

K

N

N

S

S

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S

S

V

M

F

A

G

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F

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I

G
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G

N
x
C

G
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G

S
x
T

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S

A

E

Y

F

F

Q

Q

A

L

L

E

A

A

F

L

H

R

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R

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L

F

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D

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D
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D

I
x
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D
x
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A

E

K

K

A

A

T

A

A

K

K

K

L

F

A

V

A

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N

Y

L

C

K

E

A

D

F

R

P

G

A

-

I

-

K

-

V

-

I

-

L

I

A

S

G

A

S

S

V

V

-

Q

-

P

A

A

E

E

A

E

V

A

K

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G

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A

C

D

D

I

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L

T

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T

T

V
x
T

T
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T

A
x
P

D

S

K

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A

-

Y

-

A

K

T

P

I
x
V

L

V

T

K

D

A

E

E

M

W

I

V

E

E

P

E

G

G

M

T

H

H

L

I

N

N

A

A

V
x
I

G
|
G

G

A

D
|
D

C

G

P

P

G

G

K
|
K

T

Q

E

E

L

L

D

D

R

V

R

E

I

I

V

L

E

K

R

K

A

A

R

K

V

I

I

V

V

V

E

D

Y

D

E

L

P

E

Q

Q

S

A

R

K

I

H

E

G

G

G

E

E

I

I

Q

N

H

V

M

A

-

V

-

S

-

K

-

G

-

V

-

I

-

G

P

V

E

E

D

D

S

V

P

H

V

A

T

T

E

-

L

I

W

G

Q

E

V

V

I

I

N

A

G

G

Q

L

Q

K

P

D

G

G

R

R

E

E

N

S

A

D

R

E

Q

E

V

I

T

T

L

I

F

F

D

D

S
|
S

V
x
T

G
|
G

F

L

A

A

I

I

E

Q

D

D

Y

V

S

A

A

V

L

A

R

K

Y

V

V

V

L

Y

D

E

V

N

A

A

K

L

A

S

L

K

D

N

V

V

G

G

S

S

D

K

L

I

E

K

L

F

active site: R52 (≠ E56), D219 (= D228)
binding nicotinamide-adenine-dinucleotide: R108 (= R112), T109 (= T113), G132 (= G136), C133 (≠ N137), G134 (= G138), T135 (≠ S139), Q136 (= Q140), Y156 (≠ F160), D157 (= D161), V158 (≠ I162), R159 (≠ D163), T194 (≠ V201), T195 (= T202), P196 (≠ A203), V201 (≠ I210), I216 (≠ V225), G217 (= G226), D219 (= D228), K223 (= K232), S290 (= S293), T291 (≠ V294), G292 (= G295)

O28608 Alanine dehydrogenase; AlaDH; EC 1.4.1.1 from Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16) (see paper)
34% identity, 78% coverage: 53:322/348 of query aligns to 49:319/322 of O28608

query
sites
O28608

G

G

V

D

I

L

E

R

L

A

M

M

P

P

V

A

S

H

D

L

A

M

S

G

L

Y

Y

A

A

G

F

L

K

K

Y

W

V

V

N

N

G

S

H

H

P

P

K

G

N

N

T

P

L

D

A

K

G

G

M

L

L

P

T

T

V

V

M

M

A

A

F

L

G

M

A

I

L

L

G

N

D

S

V

P

D

E

T

T

G

G

L

F

P

P

V

L

L

A

L

V

A

M

E

D

M

A

T

T

L

Y

T

T

A

S

I

L

R
|
R

T

T

A

G

A

A

T

A

S

G

A

G

L

I

V

A

A

A

R

K

Y

Y

L

L

A

A

R

R

D

K

N

N

S

S

R

S

S

V

M

F

A

G

L

F

I

I

G

G

N

C

G

G

S
x
T

Q
|
Q

S

A

E

Y

F

F

Q

Q

A

L

L

E

A

A

F

L

H

R

A

R

M

V

L

F

G

D

I

I

T

G

E

E

I

V

R

K

L

A

F

Y

D
|
D

I
x
V

D
x
R

A

E

K

K

A

A

T

A

A

K

K

K

L

F

A

V

A

S

N

Y

L

C

K

E

A

D

F

R

P

G

A

-

I

-

K

-

V

-

I

-

L

I

A

S

G

A

S

S

V

V

-

Q

-

P

A

A

E

E

A

E

V

A

K

S

G

R

A

C

D

D

I

V

V

L

T

V

T

T

V

T

T

T

A

P

D

S

K

R

A

-

Y

-

A

K

T

P

I

V

L

V

T

K

D

A

E

E

M

W

I

V

E

E

P

E

G

G

M

T

H

H

L

I

N

N

A

A

V

I

G
|
G

G
x
A

D
|
D

C

G

P

P

G

G

K
|
K

T

Q

E

E

L

L

D

D

R

V

R

E

I

I

V

L

E

K

R

K

A

A

R

K

V

I

I

V

V

V

E

D

Y

D

E

L

P

E

Q

Q

S

A

R

K

I

H

E

G

G

G

E

E

I

I

Q

N

H

V

M

A

-

V

-

S

-

K

-

G

-

V

-

I

-

G

P

V

E

E

D

D

S

V

P

H

V

A

T

T

E

-

L

I

W

G

Q

E

V

V

I

I

N

A

G

G

Q

L

Q

K

P

D

G

G

R

R

E

E

N

S

A

D

R

E

Q

E

V

I

T

T

L

I

F

F

D

D

S
|
S

V

T

G

G

F

L

A

A

I

I

E

Q

D

D

Y

V

S

A

A

V

L

A

R

K

Y

V

V

V

L

Y

D

E

V

N

A

A

K

L

A

S

L

K

D

N

V

V

G

G

S

S

D

K

L

I

E

K

L

F

R108 (= R112) binding
TQ 135:136 (≠ SQ 139:140) binding
DVR 157:159 (≠ DID 161:163) binding
GAD 217:219 (≠ GGD 226:228) binding
K223 (= K232) binding
S290 (= S293) binding

6t3eB Structure of thermococcus litoralis delta(1)-pyrroline-2-carboxylate reductase in complex with nadh and l-proline (see paper)
33% identity, 78% coverage: 52:322/348 of query aligns to 48:325/325 of 6t3eB

query
sites
6t3eB

D

N

G

G

V

F

I

I

E
x
L

L

Y

M
|
M

P

P

-

S

-

Y

V

L

S

E

D

D

A

S

S

E

L

A

Y

L

A

A

F

V

K
|
K

Y

V

V
|
V

N

S

G
x
L

H

Y

P

P

K

E

N

N

T

T

L

K

A

K

G

G

M

L

L

P

T
x
S

V
|
V

M

L

A

A

F

S

G

I

A

L

L

L

G

N

D

D

V

P

D

K

T

T

G

G

L

A

P

P

V

L

L

A

L

L

A

M

E

E

M

G

T

T

L

F

T

I

T
|
T

A

A

I

M

R
|
R

T
|
T

A

G

A

A

T

A

S

S

A

G

L

V

V

A

A

T

R

K

Y

Y

L

L

A

A

R

R

D

K

N

D

S

S

R

K

S

I

M

A

A

G

L

I

I

I

G
|
G

N
x
A

G
|
G

S
x
V

Q
|
Q

S

A

E

R

F

T

Q

Q

A

L

L

W

A

A

F

V

H

C

A

E

M

V

L

R

G

N

I

I

T

E

E

K

I

A

R

L

L

V

F
x
Y

D
|
D

I
|
I

D

N

A

P

K

K

A
x
N

T

A

A

K

K

K

L

F

A

A

A

E

N

E

L

M

K

S

A

K

F

K

P

L

A

G

I

I

K

E

V

I

I

K

L

T

A

V

G

E

S

S

V

A

A

R

E

E

A

A

V

T

K

E

G

K

A

S

D

D

I

I

V

L

T

-

V

I

T

V

A
|
A

D
x
T

K
x
T

A
|
A

Y

R

A

E

T

P

I
x
V

L

V

T

K

D

G

E

G

M

W

I

I

E

R

P

E

G

G

M

T

H

H

L

I

N

N

A

S

V
|
V

G
|
G

G
x
W

D

V

C

G

P

R

G

D

K

A

T

R

E

E

L

L

D

D

R

S

R

E

I

T

V

V

E

R

R

K

A

S

R

K

V

L

I

V

V

V

E

D

Y

S

E

K

P

E

Q

G

S

V

R

L

I

N

E

E

G

S

E

G

I

D

Q

I

H

I

M

I

P

P

E

M

D

K

S

E

P

G

V

V

-

I

-

D

-

E

-

G

-

H

-

I

-

H

T

A

E

E

L

L

W

A

Q

E

V

I

I

V

N

A

G

G

Q

V

Q

K

P

K

G

G

R

R

E

E

N

N

A

N

R

R

Q

E

V

I

T

T

L

L

F

F

D

K

S
|
S

V
|
V

G
|
G

F

L

A

A

I

I

E

E

D

D

Y

-

S

-

A

A

L

I

R

T

Y

A

V

K

L

L

D

A

V

Y

A

E

K

K

A

A

L

L

D

E

-

H

-

G

V

V

G

G

S

T

D

N

L

V

E

E

L

L

binding 1,4-dihydronicotinamide adenine dinucleotide: L71 (≠ G73), S82 (≠ T84), V83 (= V85), T107 (= T109), R110 (= R112), T111 (= T113), G134 (= G136), A135 (≠ N137), G136 (= G138), V137 (≠ S139), Q138 (= Q140), Y158 (≠ F160), D159 (= D161), I160 (= I162), N164 (≠ A166), A199 (= A203), T200 (≠ D204), T201 (≠ K205), A202 (= A206), V206 (≠ I210), V221 (= V225), G222 (= G226), W223 (≠ G227), S296 (= S293), V297 (= V294), G298 (= G295)
binding proline: L52 (≠ E56), M54 (= M58), K67 (= K69), V69 (= V71), V83 (= V85), R110 (= R112), W223 (≠ G227), V297 (= V294)

Sites not aligning to the query:

binding proline: 39

5yu4A Structural basis for recognition of l-lysine, l-ornithine, and l-2,4- diamino butyric acid by lysine cyclodeaminase (see paper)
34% identity, 76% coverage: 53:317/348 of query aligns to 60:325/344 of 5yu4A

query
sites
5yu4A

G

G

V

I

I

W

E
|
E

L

W

M
|
M

P

P

V

H

S

R

D

E

A

P

S

G

L

D

Y

H

-

I

A

T

F

L

K
|
K

Y

T

V

V

N

G

G
x
Y

H

S

P

P

K

A

N

N

T

P

L

A

A

R

-

F

G

G

M

L

L

P

T
|
T

V
x
I

M

L

A

G

F

T

G

V

A

A

L

R

G

Y

D

D

V

D

D

T

T

T

G

G

L

A

P

L

V

T

L

A

L

L

A

M

E

D

M

G

T

V

L

L

T

L

T
|
T

A

A

I

L

R
|
R

T
|
T

A

G

A

A

T

A

S

S

A

A

L

V

V

A

A

S

R

R

Y

L

L

L

A

A

R

R

D

P

N

D

S

S

R

H

S

T

M

L

A

G

L

L

I
|
I

G
|
G

N

T

G
|
G

S
x
A

Q
|
Q

S

A

E

V

F

T

Q

Q

A

L

L

H

A

A

F

L

H

S

A

L

M

V

L

L

G

P

I

L

T

Q

E

R

I

A

R

L

L

V

F

W

D
|
D

I
x
T

D
|
D

A

-

K

P

A

A

T
x
H

A

R

K

E

L

S

A

F

A

A

N

R

L

R

K

A

A

A

F

F

P

T

A

G

I

V

K

S

V

V

I

E

L

I

A

A

G

-

S

E

V

P

A

A

E

R

A

I

V

A

K

A

G

E

A

A

D

D

I

V

V

I

T

S

T

T

V
x
A

T
|
T

A
x
S

D
x
V

K

A

-

V

A

G

Y

Q

A

G

T

P

I
x
V

L

L

T

P

D

D

E

T

M

G

I

V

E

R

P

E

G

H

M

L

H

H

L

I

N

N

A

A

V
|
V

G
|
G

G
x
A

D
|
D

C

L

P

V

G

G

K
|
K

T

T

E

E

L

L

D

P

R

L

R

G

I

L

V

L

E

E

R

R

A

A

R

F

V

V

I

T

V

A

E

D

Y

H

E

P

P

E

Q

Q

S

A

R

L

I

R

E

E

G

G

E

E

I

C

Q

Q

H

Q

M

L

P

S

E

A

D

D

S

R

P

L

V

G

T

P

E

Q

L

L

W

A

Q

H

V

L

I

C

N

-

G

-

Q

A

Q

D

P

P

G

A

R

A

E

A

N

A

A

G

R

R

Q

Q

V

D

T

T

L

L

-

S

-

V

F

F

D

D

S
|
S

V
x
T

G
|
G

F

F

A

A

I

F

E

E

D

D

Y

A

S

L

A

A

L

M

R

E

Y

V

V

F

L

L

D

E

V

A

A

A

K

A

A

E

L

R

D

D

V

L

G

G

binding 2,4-diaminobutyric acid: E63 (= E56), M65 (= M58), K77 (= K69), R121 (= R112), A235 (≠ G227), D236 (= D228), T302 (≠ V294), G303 (= G295)
binding nicotinamide-adenine-dinucleotide: Y81 (≠ G73), T93 (= T84), I94 (≠ V85), T118 (= T109), R121 (= R112), T122 (= T113), I144 (= I135), G145 (= G136), G147 (= G138), A148 (≠ S139), Q149 (= Q140), D170 (= D161), T171 (≠ I162), D172 (= D163), H175 (≠ T167), A208 (≠ V201), T209 (= T202), S210 (≠ A203), V211 (≠ D204), V218 (≠ I210), V233 (= V225), G234 (= G226), A235 (≠ G227), D236 (= D228), K240 (= K232), S301 (= S293), T302 (≠ V294), G303 (= G295)

Sites not aligning to the query:

binding 2,4-diaminobutyric acid: 49

5yu3A Structural basis for recognition of l-lysine, l-ornithine, and l-2,4- diamino butyric acid by lysine cyclodeaminase (see paper)
34% identity, 76% coverage: 53:317/348 of query aligns to 60:325/344 of 5yu3A

query
sites
5yu3A

G

G

V

I

I

W

E
|
E

L

W

M
|
M

P

P

V

H

S

R

D

E

A

P

S

G

L

D

Y

H

-

I

A

T

F

L

K
|
K

Y

T

V
|
V

N

G

G
x
Y

H

S

P

P

K

A

N

N

T

P

L

A

A

R

-

F

G

G

M

L

L

P

T
|
T

V
x
I

M

L

A

G

F

T

G

V

A

A

L

R

G

Y

D

D

V

D

D

T

T

T

G

G

L

A

P

L

V

T

L

A

L

L

A

M

E

D

M

G

T

V

L

L

T

L

T
|
T

A

A

I

L

R
|
R

T
|
T

A

G

A

A

T

A

S

S

A

A

L

V

V

A

A

S

R

R

Y

L

L

L

A

A

R

R

D

P

N

D

S

S

R

H

S

T

M

L

A

G

L

L

I
|
I

G
|
G

N

T

G
|
G

S
x
A

Q
|
Q

S

A

E

V

F

T

Q

Q

A

L

L

H

A

A

F

L

H

S

A

L

M

V

L

L

G

P

I

L

T

Q

E

R

I

A

R

L

L

V

F

W

D
|
D

I
x
T

D
|
D

A

-

K

P

A

A

T
x
H

A

R

K

E

L

S

A

F

A

A

N

R

L

R

K

A

A

A

F

F

P

T

A

G

I

V

K

S

V

V

I

E

L

I

A

A

G

-

S

E

V

P

A

A

E

R

A

I

V

A

K

A

G

E

A

A

D

D

I

V

V

I

T

S

T

T

V
x
A

T
|
T

A
x
S

D
x
V

K

A

-

V

A

G

Y

Q

A

G

T

P

I
x
V

L

L

T

P

D

D

E

T

M

G

I

V

E

R

P

E

G

H

M

L

H

H

L

I

N

N

A

A

V
|
V

G
|
G

G
x
A

D
|
D

C

L

P

V

G

G

K
|
K

T

T

E

E

L

L

D

P

R

L

R

G

I

L

V

L

E

E

R

R

A

A

R

F

V

V

I

T

V

A

E

D

Y

H

E

P

P

E

Q

Q

S

A

R

L

I

R

E

E

G

G

E

E

I

C

Q

Q

H

Q

M

L

P

S

E

A

D

D

S

R

P

L

V

G

T

P

E

Q

L

L

W

A

Q

H

V

L

I

C

N

-

G

-

Q

A

Q

D

P

P

G

A

R

A

E

A

N

A

A

G

R

R

Q

Q

V

D

T

T

L

L

-

S

-

V

F

F

D

D

S
|
S

V
x
T

G
|
G

F

F

A

A

I

F

E

E

D

D

Y

A

S

L

A

A

L

M

R

E

Y

V

V

F

L

L

D

E

V

A

A

A

K

A

A

E

L

R

D

D

V

L

G

G

binding nicotinamide-adenine-dinucleotide: Y81 (≠ G73), T93 (= T84), I94 (≠ V85), T118 (= T109), R121 (= R112), T122 (= T113), I144 (= I135), G145 (= G136), G147 (= G138), A148 (≠ S139), Q149 (= Q140), D170 (= D161), T171 (≠ I162), D172 (= D163), H175 (≠ T167), A208 (≠ V201), T209 (= T202), S210 (≠ A203), V211 (≠ D204), V218 (≠ I210), V233 (= V225), G234 (= G226), A235 (≠ G227), D236 (= D228), K240 (= K232), S301 (= S293), T302 (≠ V294), G303 (= G295)
binding proline: E63 (= E56), M65 (= M58), K77 (= K69), V79 (= V71), R121 (= R112), A235 (≠ G227), T302 (≠ V294), G303 (= G295)

Sites not aligning to the query:

binding proline: 49

5gzlA Cyclodeaminase_pa
34% identity, 76% coverage: 53:317/348 of query aligns to 64:329/357 of 5gzlA

query
sites
5gzlA

G

G

V
x
I

I

W

E
|
E

L

W

M

M

P

P

V

H

S

R

D

E

A

P

S

G

L

D

Y

H

-

I

A

T

F

L

K

K

Y

T

V

V

N

G

G
x
Y

H

S

P

P

K

A

N

N

T

P

L

A

A

R

-

F

G

G

M

L

L

P

T
|
T

V
x
I

M

L

A

G

F

T

G

V

A

A

L

R

G

Y

D

D

V

D

D

T

T

T

G

G

L

A

P

L

V

T

L

A

L

L

A

M

E

D

M

G

T

V

L

L

T

L

T
|
T

A

A

I

L

R
|
R

T
|
T

A

G

A

A

T

A

S

S

A

A

L

V

V

A

A

S

R

R

Y

L

L

L

A

A

R

R

D

P

N

D

S

S

R

H

S

T

M

L

A

G

L

L

I
|
I

G
|
G

N

T

G
|
G

S
x
A

Q
|
Q

S

A

E

V

F

T

Q

Q

A

L

L

H

A

A

F

L

H

S

A

L

M

V

L

L

G

P

I

L

T

Q

E

R

I

A

R

L

L

V

F

W

D
|
D

I
x
T

D
|
D

A

-

K

P

A

A

T
x
H

A

R

K

E

L

S

A

F

A

A

N

R

L

R

K

A

A

A

F

F

P

T

A

G

I

V

K

S

V

V

I

E

L

I

A

A

G

-

S

E

V

P

A

A

E

R

A

I

V

A

K

A

G

E

A

A

D

D

I

V

V

I

T

S

T

T

V
x
A

T
|
T

A
x
S

D
x
V

K

A

-

V

A

G

Y

Q

A

G

T

P

I
x
V

L

L

T

P

D

D

E

T

M

G

I

V

E

R

P

E

G

H

M

L

H

H

L

I

N

N

A

A

V
|
V

G
|
G

G
x
A

D
|
D

C

L

P

V

G

G

K
|
K

T

T

E

E

L

L

D

P

R

L

R

G

I

L

V

L

E

E

R

R

A

A

R

F

V

V

I

T

V

A

E

D

Y

H

E

P

P

E

Q

Q

S

A

R

L

I
x
R

E
|
E

G

G

E

E

I

C

Q

Q

H

Q

M

L

P

S

E

A

D

D

S

R

P

L

V

G

T

P

E

Q

L

L

W

A

Q

H

V

L

I

C

N

-

G

-

Q

A

Q

D

P

P

G

A

R

A

E

A

N

A

A

G

R

R

Q

Q

V

D

T

T

L

L

-

S

-

V

F

F

D

D

S
|
S

V
x
T

G
|
G

F

F

A

A

I

F

E

E

D

D

Y

A

S

L

A

A

L

M

R

E

Y

V

V

F

L

L

D

E

V

A

A

A

K

A

A

E

L

R

D

D

V

L

G

G

binding lysine: I65 (≠ V54), E67 (= E56), D240 (= D228), R267 (≠ I255), E268 (= E256)
binding nicotinamide-adenine-dinucleotide: Y85 (≠ G73), T97 (= T84), I98 (≠ V85), T122 (= T109), R125 (= R112), T126 (= T113), I148 (= I135), G149 (= G136), G151 (= G138), A152 (≠ S139), Q153 (= Q140), D174 (= D161), T175 (≠ I162), D176 (= D163), H179 (≠ T167), A212 (≠ V201), T213 (= T202), S214 (≠ A203), V215 (≠ D204), V222 (≠ I210), V237 (= V225), G238 (= G226), A239 (≠ G227), D240 (= D228), K244 (= K232), S305 (= S293), T306 (≠ V294), G307 (= G295)

Sites not aligning to the query:

binding lysine: 53, 54, 55, 57

5gziA Cyclodeaminase_pa
34% identity, 76% coverage: 53:317/348 of query aligns to 64:329/354 of 5gziA

query
sites
5gziA

G

G

V

I

I

W

E
|
E

L

W

M
|
M

P

P

V

H

S

R

D

E

A

P

S

G

L

D

Y

H

-

I

A

T

F

L

K
|
K

Y

T

V

V

N

G

G
x
Y

H

S

P

P

K

A

N

N

T

P

L

A

A

R

-

F

G

G

M

L

L

P

T
|
T

V
x
I

M

L

A

G

F

T

G

V

A

A

L

R

G

Y

D

D

V

D

D

T

T

T

G

G

L

A

P

L

V

T

L

A

L

L

A

M

E

D

M

G

T

V

L

L

T

L

T
|
T

A

A

I

L

R
|
R

T
|
T

A

G

A

A

T

A

S

S

A

A

L

V

V

A

A

S

R

R

Y

L

L

L

A

A

R

R

D

P

N

D

S

S

R

H

S

T

M

L

A

G

L

L

I
|
I

G
|
G

N

T

G
|
G

S
x
A

Q
|
Q

S

A

E

V

F

T

Q

Q

A

L

L

H

A

A

F

L

H

S

A

L

M

V

L

L

G

P

I

L

T

Q

E

R

I

A

R

L

L

V

F

W

D
|
D

I
x
T

D
|
D

A

-

K

P

A

A

T
x
H

A

R

K

E

L

S

A

F

A

A

N

R

L

R

K

A

A

A

F

F

P

T

A

G

I

V

K

S

V

V

I

E

L

I

A

A

G

-

S

E

V

P

A

A

E

R

A

I

V

A

K

A

G

E

A

A

D

D

I

V

V

I

T

S

T

T

V
x
A

T
|
T

A
x
S

D
x
V

K

A

-

V

A

G

Y

Q

A

G

T

P

I
x
V

L

L

T

P

D

D

E

T

M

G

I

V

E

R

P

E

G

H

M

L

H

H

L

I

N

N

A

A

V
|
V

G
|
G

G
x
A

D
|
D

C

L

P

V

G

G

K
|
K

T

T

E

E

L

L

D

P

R

L

R

G

I

L

V

L

E

E

R

R

A

A

R

F

V

V

I

T

V

A

E

D

Y

H

E

P

P

E

Q

Q

S

A

R

L

I

R

E

E

G

G

E

E

I

C

Q

Q

H

Q

M

L

P

S

E

A

D

D

S

R

P

L

V

G

T

P

E

Q

L

L

W

A

Q

H

V

L

I

C

N

-

G

-

Q

A

Q

D

P

P

G

A

R

A

E

A

N

A

A

G

R

R

Q

Q

V

D

T

T

L

L

-

S

-

V

F

F

D

D

S
|
S

V
x
T

G
|
G

F

F

A

A

I

F

E

E

D

D

Y

A

S

L

A

A

L

M

R

E

Y

V

V

F

L

L

D

E

V

A

A

A

K

A

A

E

L

R

D

D

V

L

G

G

binding nicotinamide-adenine-dinucleotide: Y85 (≠ G73), T97 (= T84), I98 (≠ V85), T122 (= T109), R125 (= R112), T126 (= T113), I148 (= I135), G149 (= G136), G151 (= G138), A152 (≠ S139), Q153 (= Q140), D174 (= D161), T175 (≠ I162), D176 (= D163), H179 (≠ T167), A212 (≠ V201), T213 (= T202), S214 (≠ A203), V215 (≠ D204), V222 (≠ I210), V237 (= V225), G238 (= G226), A239 (≠ G227), D240 (= D228), K244 (= K232), S305 (= S293), T306 (≠ V294), G307 (= G295)
binding (2S)-piperidine-2-carboxylic acid: E67 (= E56), M69 (= M58), K81 (= K69), R125 (= R112), A239 (≠ G227), T306 (≠ V294), G307 (= G295)

Sites not aligning to the query:

binding (2S)-piperidine-2-carboxylic acid: 53

6rqaB Crystal structure of the iminosuccinate reductase of paracoccus denitrificans in complex with NAD+ (see paper)
31% identity, 73% coverage: 70:322/348 of query aligns to 74:322/322 of 6rqaB

query
sites
6rqaB

Y

W

V

P

N
|
N

G

N

H

Q

P

K

K

H

N

N

T

L

L

I

A

N

G

H

M

Q

L

S

T

T

V

V

M

F

A

L

F

F

G

-

A

-

L

-

G

-

D

D

V

P

D

D

T

T

G

G

L

R

P

V

V

S

L

A

A

V

E

G

M

G

T

N

L

L

T

L

T

T

A

A

I

L

R
|
R

T
|
T

A

A

T

A

S

S

A

A

L

V

V

S

A

I

R

K

Y

Y

L

L

A

A

R

P

D

K

N

G

S

A

R

K

S

V

M

L

A

G

L

M

I

I

G
|
G

N

A

G
|
G

S
x
H

Q
|
Q

S

S

E

A

F

F

Q

Q

A

M

L

R

A

A

F

A

H

A

A

N

M

V

L

H

G

R

I

F

T

E

E

K

I

V

R

I

L

G

F

W

D
x
N

I
x
P

D
x
H

A

P

K

E

A

M

T

L

A

S

K

R

L

L

A

A

-

D

-

T

-

A

A

A

N

E

L

L

K

G

-

L

A

P

F

F

P

E

A

A

I

V

K

E

V

L

I

D

L

R

A

L

G

G

S

A

V

E

A

A

E

-

A

-

V

-

K

-

G

-

A

-

D

D

I

V

V

I

T

V

T

S

V
x
I

T
|
T

A
x
S

D

-

K

-

A
x
S

Y

F

A

S

T

P

I

L

L

L

T

M

D

N

E

E

M

H

I

V

E

K

P

G

G

P

M

T

H

H

L

I

N

A

A

A

V
x
M

G
|
G

G

T

D
|
D

C

T

P

K

G

G

K
|
K

T

Q

E

E

L

L

D

D

R

P

R

A

I

L

V

V

E

A

R

R

A

A

R

R

V

I

I

F

V

T

E

D

Y

E

E

V

P

A

Q

Q

S

S

R

V

I

S

E

I

G

G

E

E

I

C

Q

Q

H

H

-

A

-

I

-

A

-

G

M

L

P

I

E

R

D

E

S

D

P

Q

V

V

T

G

E

E

L

L

W

G

Q

A

V

V

I

V

N

A

G

G

Q

D

P

P

G

G

R

R

E

G

N

D

A

A

R

-

Q

E

V

V

T

T

L

I

F

F

D

D

S
x
G

V
x
T

G
|
G

F

V

A

G

I

L

E

Q

D

D

Y

L

S

A

A

V

L

A

R

Q

Y

A

V

V

L

V

D

E

V

L

A

A

K

K

A

H

L

K

D

G

V

V

G

A

S

Q

D

E

L

V

E

E

L

I

binding Tb-Xo4: N76 (= N72)
binding nicotinamide-adenine-dinucleotide: R112 (= R112), T113 (= T113), G136 (= G136), G138 (= G138), H139 (≠ S139), Q140 (= Q140), N161 (≠ D161), P162 (≠ I162), H163 (≠ D163), I199 (≠ V201), T200 (= T202), S201 (≠ A203), S202 (≠ A206), M221 (≠ V225), G222 (= G226), D224 (= D228), K228 (= K232), G293 (≠ S293), T294 (≠ V294), G295 (= G295)

Sites not aligning to the query:

binding Tb-Xo4: 48, 49, 50

6rqaA Crystal structure of the iminosuccinate reductase of paracoccus denitrificans in complex with NAD+ (see paper)
31% identity, 73% coverage: 70:322/348 of query aligns to 74:322/322 of 6rqaA

query
sites
6rqaA

Y

W

V

P

N

N

G

N

H

Q

P

K

K

H

N

N

T

L

L

I

A

N

G
x
H

M

Q

L

S

T

T

V

V

M

F

A

L

F

F

G

-

A

-

L

-

G

-

D

D

V

P

D

D

T

T

G

G

L

R

P

V

V

S

L

A

A

V

E

G

M

G

T

N

L

L

T

L

T

T

A

A

I

L

R

R

T
|
T

A

A

T

A

S

S

A

A

L

V

V

S

A

I

R

K

Y

Y

L

L

A

A

R

P

D

K

N

G

S

A

R

K

S

V

M

L

A

G

L

M

I

I

G

G

N

A

G
|
G

S
x
H

Q
|
Q

S

S

E

A

F

F

Q

Q

A

M

L

R

A

A

F

A

H

A

A

N

M

V

L

H

G

R

I

F

T

E

E

K

I

V

R

I

L

G

F

W

D
x
N

I
x
P

D
x
H

A

P

K

E

A
x
M

T

L

A

S

K

R

L

L

A

A

-

D

-

T

-

A

A

A

N

E

L

L

K

G

-

L

A

P

F

F

P

E

A

A

I

V

K

E

V

L

I

D

L

R

A

L

G

G

S

A

V

E

A

A

E

-

A

-

V

-

K

-

G

-

A

-

D

D

I

V

V

I

T

V

T

S

V
x
I

T
|
T

A
x
S

D

-

K

-

A
x
S

Y

F

A

S

T

P

I

L

L

L

T

M

D

N

E

E

M

H

I

V

E

K

P

G

G

P

M

T

H

H

L

I

N

A

A

A

V
x
M

G
|
G

G

T

D
|
D

C

T

P

K

G

G

K
|
K

T

Q

E

E

L

L

D

D

R

P

R

A

I

L

V

V

E

A

R

R

A

A

R

R

V

I

I

F

V

T

E

D

Y

E

E

V

P

A

Q

Q

S

S

R

V

I

S

E

I

G

G

E

E

I

C

Q

Q

H

H

-

A

-

I

-

A

-

G

M

L

P

I

E

R

D

E

S

D

P

Q

V

V

T

G

E

E

L

L

W

G

Q

A

V

V

I

V

N

A

G

G

Q

D

P

P

G

G

R

R

E

G

N

D

A

A

R

-

Q

E

V

V

T

T

L

I

F

F

D

D

S
x
G

V
x
T

G
|
G

F

V

A

G

I

L

E

Q

D

D

Y

L

S

A

A

V

L

A

R

Q

Y

A

V

V

L

V

D

E

V

L

A

A

K

K

A

H

L

K

D

G

V

V

G

A

S

Q

D

E

L

V

E

E

L

I

binding nicotinamide-adenine-dinucleotide: H85 (≠ G81), T113 (= T113), G138 (= G138), H139 (≠ S139), Q140 (= Q140), N161 (≠ D161), P162 (≠ I162), H163 (≠ D163), M166 (≠ A166), I199 (≠ V201), T200 (= T202), S201 (≠ A203), S202 (≠ A206), M221 (≠ V225), G222 (= G226), D224 (= D228), K228 (= K232), G293 (≠ S293), T294 (≠ V294), G295 (= G295)

A1B8Z0 Iminosuccinate reductase; EC 1.4.1.- from Paracoccus denitrificans (strain Pd 1222) (see paper)
31% identity, 73% coverage: 70:322/348 of query aligns to 72:320/320 of A1B8Z0

query
sites
A1B8Z0

Y

W

V

P

N

N

G

N

H

Q

P

K

K

H

N

N

T

L

L

I

A

N

G

H

M

Q

L

S

T

T

V

V

M

F

A

L

F

F

G

-

A

-

L

-

G

-

D

D

V

P

D

D

T

T

G

G

L

R

P

V

V

S

L

A

A

V

E

G

M

G

T

N

L

L

T

L

T

T

A

A

I

L

R
|
R

T

T

A

A

T

A

S

S

A

A

L

V

V

S

A

I

R

K

Y

Y

L

L

A

A

R

P

D

K

N

G

S

A

R

K

S

V

M

L

A

G

L

M

I

I

G

G

N

A

G

G

S
x
H

Q
|
Q

S

S

E

A

F

F

Q

Q

A

M

L

R

A

A

F

A

H

A

A

N

M

V

L

H

G

R

I

F

T

E

E

K

I

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R

I

L

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F

W

D
x
N

I

P

D

H

A

P

K

E

A

M

T

L

A

S

K

R

L

L

A

A

-

D

-

T

-

A

A

A

N

E

L

L

K

G

-

L

A

P

F

F

P

E

A

A

I

V

K

E

V

L

I

D

L

R

A

L

G

G

S

A

V

E

A

A

E

-

A

-

V

-

K

-

G

-

A

-

D

D

I

V

V

I

T

V

T

S

V

I

T

T

A
x
S

D

-

K

-

A

S

Y

F

A

S

T

P

I

L

L

L

T

M

D

N

E

E

M

H

I

V

E

K

P

G

G

P

M

T

H

H

L

I

N

A

A

A

V
x
M

G
|
G

G
x
T

D
|
D

C

T

P

K

G

G

K
|
K

T

Q

E

E

L

L

D

D

R

P

R

A

I

L

V

V

E

A

R

R

A

A

R

R

V

I

I

F

V

T

E

D

Y

E

E

V

P

A

Q

Q

S

S

R

V

I

S

E

I

G

G

E

E

I

C

Q

Q

H

H

-

A

-

I

-

A

-

G

M

L

P

I

E

R

D

E

S

D

P

Q

V

V

T

G

E

E

L

L

W

G

Q

A

V

V

I

V

N

A

G

G

Q

D

P

P

G

G

R

R

E

G

N

D

A

A

R

-

Q

E

V

V

T

T

L

I

F

F

D

D

S
x
G

V

T

G

G

F

V

A

G

I

L

E

Q

D

D

Y

L

S

A

A

V

L

A

R

Q

Y

A

V

V

L

V

D

E

V

L

A

A

K

K

A

H

L

K

D

G

V

V

G

A

S

Q

D

E

L

V

E

E

L

I

R110 (= R112) binding
HQ 137:138 (≠ SQ 139:140) binding
N159 (≠ D161) binding
S199 (≠ A203) binding
MGTD 219:222 (≠ VGGD 225:228) binding
K226 (= K232) binding
G291 (≠ S293) binding

Q14894 Ketimine reductase mu-crystallin; NADP-regulated thyroid-hormone-binding protein; EC 1.5.1.25 from Homo sapiens (Human) (see paper)
32% identity, 60% coverage: 101:309/348 of query aligns to 106:308/314 of Q14894

query
sites
Q14894

L

L

A

A

E

V

M

M

-

D

-

G

T

N

L

V

T

I

T

T

A

A

I

K

R

R

T

T

A

A

T

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S

A

A

L

I

V

A

A

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R

K

Y

F

L

L

A

K

R

P

D

P

N

S

S

S

R

E

S

V

M

L

A

C

L

I

I

L

G
|
G

N
x
A

G
|
G

S
x
V

Q
|
Q

S
x
A

E

Y

F

S

Q

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A

Y

L

E

A

I

F

F

H

T

A

E

M

Q

L

F

G

S

I

F

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K

E

E

I

V

R

R

L

I

F

W

D
x
N

I
x
R

D

T

A

K

K

E

A

N

T

A

A

E

K

K

L

F

A

A

A

D

N

T

L

V

K

Q

A

G

F

-

P

-

A

-

I

-

K

E

V

V

I

R

L

V

A

C

G

S

S

S

V

V

A

Q

E

E

A

A

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K

A

G

G

A

A

D

D

I

V

V

I

T

I

T

T

V

V

T

T

A

L

D

-

K

-

A

A

Y

T

A

E

T

P

I

I

L

L

T

F

D

G

E

E

M

W

I

V

E

K

P

P

G

G

M

A

H

H

L

I

N

N

A

A

V

V

G

G

G

A

D

S

C

R

P

P

G

D

K

W

T

R

E

E

L

L

D

D

R

D

R

E

I

L

V

M

E

K

R

E

A

A

R

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V

L

I

Y

V

V

E

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Y

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E

Q

P

E

Q

A

S

A

R

L

I

K

E

E

G

S

E

G

I

D

Q

V

H

L

M

L

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S

E

G

D

A

S

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P

I

V

F

T

A

E

E

L

L

W

G

Q

E

V

V

I

I

N

K

G

G

Q

V

Q

K

P

P

G

A

R

-

E

-

N

H

A

C

R

E

Q

K

V

T

T

T

L

V

F

F

D

K

S

S

V

L

G

G

F

M

A

A

I

V

E

E

D

D

Y

T

S

V

A

A

L

A

R

K

Y

L

V

I

L

Y

D

D

GAGVQA 143:148 (≠ GNGSQS 136:141) binding
N168 (≠ D161) binding
R169 (≠ I162) binding

2i99A Crystal structure of human mu_crystallin at 2.6 angstrom (see paper)
32% identity, 60% coverage: 101:309/348 of query aligns to 105:307/312 of 2i99A

query
sites
2i99A

L

L

A

A

E

V

M

M

-

D

-

G

T

N

L

V

T

I

T
|
T

A

A

I

K

R
|
R

T
|
T

A

A

T

V

S

S

A

A

L

I

V

A

A

T

R

K

Y

F

L

L

A

K

R

P

D

P

N

S

S

S

R

E

S

V

M

L

A

C

L

I

I

L

G
|
G

N
x
A

G
|
G

S
x
V

Q
|
Q

S

A

E

Y

F

S

Q

H

A

Y

L

E

A

I

F

F

H

T

A

E

M

Q

L

F

G

S

I

F

T

K

E

E

I

V

R

R

L

I

F

W

D
x
N

I
x
R

D
x
T

A

K

K

E

A
x
N

T

A

A

E

K

K

L

F

A

A

A

D

N

T

L

V

K

Q

A

G

F

-

P

-

A

-

I

-

K

E

V

V

I

R

L

V

A

C

G

S

S

S

V

V

A

Q

E

E

A

A

V

V

K

A

G
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