SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF3459 FitnessBrowser__psRCH2:GFF3459 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q58761 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see paper)
31% identity, 94% coverage: 15:293/298 of query aligns to 13:284/284 of Q58761

query
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Q58761

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DNE 34:36 (≠ DDE 36:38) binding
D212 (= D221) binding
STGGT 216:220 (≠ SSGRT 225:229) binding

1u9zA Crystal structure of phosphoribosyl diphosphate synthase complexed with amp and ribose 5-phosphate (see paper)
32% identity, 94% coverage: 15:293/298 of query aligns to 13:274/274 of 1u9zA

query
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1u9zA

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binding adenosine monophosphate: F32 (= F34), D34 (= D36), E36 (= E38), R92 (= R97), Q93 (= Q98), F97 (= F102), H125 (= H130)
binding ribose-5-phosphate: D163 (= D172), D202 (= D221), I205 (≠ A224), S206 (= S225), T207 (≠ S226), G208 (= G227), T210 (= T229)

Q97Z86 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus) (see paper)
31% identity, 87% coverage: 18:276/298 of query aligns to 16:271/291 of Q97Z86

query
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Q97Z86

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STGGT 220:224 (≠ SSGRT 225:229) binding

4twbA Sulfolobus solfataricus ribose-phosphate pyrophosphokinase (see paper)
31% identity, 87% coverage: 18:276/298 of query aligns to 16:258/278 of 4twbA

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4twbA

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binding adenosine monophosphate: F32 (= F34), D34 (= D36), E36 (= E38), R93 (= R97), F98 (= F102), H127 (= H130)

3mbiA Crystal structure of the phosphoribosylpyrophosphate (prpp) synthetase from thermoplasma volcanium in complex with adp-mg2+ and ribose 5- phosphate (see paper)
25% identity, 95% coverage: 15:296/298 of query aligns to 15:286/287 of 3mbiA

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3mbiA

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D

D

P

I

H
|
H

L

D

H

E

R

K

V

T

A

L

T

S

L

Y

Q

S

E

K

A

-

V

V

P

K

L

F

E

S

D

D

A

-

V

-

T

-

L

L

C

H

A

A

A

N

P

D

A

A

L

I

A

V

R

R

L

Y

I

Y

A

K

E

N

R

V

H

D

P

V

D

D

A

-

L

Y

L

V

I

V

G

S

P

P

D

D

A

D

E

G

A

G

L

L

Q

A

W

R

I

V

E

A

A

D

A

I

A

S

A

A

E

K

H

L

G

G

F

K

A

K

H

H

G

F

V

F

C

I

S

E

K
|
K

V

K

R
|
R

H

I

G

D

D

D

H

R

Q

T

V

V

E

E

I

M

A

K

L

V

P

P

E

N

L

V

Q

D

F

V

A

N

G

G

R

K

H

K

V

L

L

I

L

V

D

D

V

I

A

I

S
|
S

S
x
T

G
|
G

R

G

T
|
T

L

I

A

A

Q

K

A

S

E

G

K

L

L

L

L

R

A

E

A

K

G

G

A

A

A

S

S

K

V

I

D

Y

V

V

A

S

V

A

T

V

H

H

A

G

L

L

F

F

A

V

-

N

G

G

D

S

A

E

L

N

Q

K

V

I

I

L

R

Q

D

N

A

A

G

D

V

E

G

I

H

H

V

V

W

-

S

-

T

T

D

D

C

T

I

V

A

E

H

S

G

K

S

F

N

S

A

D

V

I

S

S

M

V

A

Y

P

Q

Q

E

L

V

A

C

E

N

A

Y

L

I

R

R

P

D

L

I

binding adenosine-5'-diphosphate: F34 (= F34), D36 (= D36), E38 (= E38), R93 (= R97), Q94 (= Q98), Y98 (≠ F102), H126 (= H130), K186 (= K195), R188 (= R197)
binding magnesium ion: H95 (≠ D99), H126 (= H130)
binding phosphate ion: R188 (= R197), S216 (= S225), T217 (≠ S226), G218 (= G227), T220 (= T229)

Q97CA5 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1) (see paper)
25% identity, 95% coverage: 15:296/298 of query aligns to 13:284/286 of Q97CA5

query
sites
Q97CA5

A

A

L

A

R

R

L

I

A

A

E

E

A

E

C

L

G

K

L

T

T

E

A

P

A

V

Q

M

I

P

E

D

R

E

H

R

R

R

F

F

P

P

D
|
D

D
x
G

E
|
E

L

L

R

Y

L

L

R

R

L

Y

P

D

L

-

A

-

D

-

G

-

E

E

A

D

I

L

A

T

E

G

Q

H

L

N

V

I

I

F

Y

I

R

I

G

G

L

N

D

T

H

H

P

S

N

D

E

A

K

E

L

V

V

M

E

E

L

M

L

I

L

L

I

T

A

L

G

S

E

A

A

I

R

Q

R

D

L

Y

G

R

A

T

K

K

R

S

L

V

V

N

L

I

V

I

T

A

P

P

Y

Y

L

Y

A

G

Y

Y

M

A

R
|
R

Q
|
Q

D
x
H

I

Q

A

R

F

Y

N

K

P

N

G

G

E

E

V

P

V

I

S

S

Q

S

R

Q

V

I

I

L

G

T

Q

E

L

I

L

Y

A

S

G

S

Q

Y

F

S

D

N

G

S

L

I

I

A

T

T

V

V

D

D

P

I

H

H

L

D

H

E

R

K

V

T

A

L

T

S

L

Y

Q

S

E

K

A

-

V

V

P

K

L

F

E

S

D

D

A

-

V

-

T

-

L

L

C

H

A

A

A

N

P

D

A

A

L

I

A

V

R

R

L

Y

I

Y

A

K

E

N

R

V

H

D

P

V

D

D

A

-

L

Y

L

V

I

V

G

S

P

P

D

D

A

D

E

G

A

G

L

L

Q

A

W

R

I

V

E

A

A

D

A

I

A

S

A

A

E

K

H

L

G

G

F

K

A

K

H

H

G

F

V

F

C

I

S

E

K

K

V

K

R
|
R

H

I

G

D

D

D

H

R

Q

T

V

V

E

E

I

M

A

K

L

V

P

P

E

N

L

V

Q

D

F

V

A

N

G

G

R

K

H

K

V

L

L

I

L

V

D
|
D

D

D

V

I

A

I

S
|
S

S
x
T

G
|
G

R
x
G

T
|
T

L

I

A

A

Q

K

A

S

E

G

K

L

L

L

L

R

A

E

A

K

G

G

A

A

A

S

S

K

V

I

D

Y

V

V

A

S

V

A

T

V

H

H

A

G

L

L

F

F

A

V

-

N

G

G

D

S

A

E

L

N

Q

K

V

I

I

L

R

Q

D

N

A

A

G

D

V

E

G

I

H

H

V

V

W

-

S

-

T

T

D

D

C

T

I

V

A

E

H

S

G

K

S

F

N

S

A

D

V

I

S

S

M

V

A

Y

P

Q

Q

E

L

V

A

C

E

N

A

Y

L

I

R

R

P

D

L

I

DGE 34:36 (≠ DDE 36:38) binding
RQH 91:93 (≠ RQD 97:99) binding
R186 (= R197) binding
D210 (= D221) binding
STGGT 214:218 (≠ SSGRT 225:229) binding

3lpnA Crystal structure of the phosphoribosylpyrophosphate (prpp) synthetase from thermoplasma volcanium in complex with an atp analog (ampcpp). (see paper)
25% identity, 95% coverage: 15:296/298 of query aligns to 13:284/284 of 3lpnA

query
sites
3lpnA

A

A

L

A

R
|
R

L

I

A

A

E

E

A

E

C

L

G

K

L

T

T

E

A

P

A

V

Q

M

I

P

E

D

R

E

H

R

R

R

F
|
F

P

P

D
|
D

D

G

E
|
E

L

L

R

Y

L

L

R

R

L

Y

P

D

L

-

A

-

D

-

G

-

E

E

A

D

I

L

A

T

E

G

Q

H

L

N

V

I

I

F

Y

I

R

I

G

G

L

N

D

T

H

H

P
x
S

N

D

E

A

K

E

L

V

V

M

E

E

L

M

L

I

L

L

I

T

A

L

G

S

E

A

A

I

R

Q

R

D

L

Y

G

R

A

T

K

K

R

S

L

V

V

N

L

I

V

I

T

A

P

P

Y

Y

L

Y

A

G

Y

Y

M

A

R
|
R

Q
|
Q

D
x
H

I

Q

A

R

F
x
Y

N

K

P

N

G

G

E

E

V

P

V

I

S

S

Q

S

R

Q

V

I

I

L

G

T

Q

E

L

I

L

Y

A

S

G

S

Q

Y

F

S

D

N

G

S

L

I

I

A

T

T

V

V

D

D

P

I

H
|
H

L
x
D

H

E

R

K

V

T

A

L

T

S

L

Y

Q

S

E

K

A

-

V

V

P

K

L

F

E

S

D

D

A

-

V

-

T

-

L

L

C

H

A

A

A

N

P

D

A

A

L

I

A

V

R

R

L

Y

I

Y

A

K

E

N

R

V

H

D

P

V

D

D

A

-

L

Y

L

V

I

V

G

S

P

P

D

D

A

D

E

G

A

G

L

L

Q

A

W

R

I

V

E

A

A

D

A

I

A

S

A

A

E

K

H

L

G

G

F

K

A

K

H

H

G

F

V

F

C

I

S

E

K

K

V

K

R

R

H

I

G

D

D

D

H

R

Q

T

V

V

E

E

I

M

A

K

L

V

P

P

E

N

L

V

Q

D

F

V

A

N

G

G

R

K

H

K

V

L

L

I

L

V

D

D

V

I

A

I

S
|
S

S
x
T

G
|
G

R

G

T
|
T

L

I

A

A

Q

K

A

S

E

G

K

L

L

L

L

R

A

E

A

K

G

G

A

A

A

S

S

K

V

I

D

Y

V

V

A

S

V

A

T

V

H

H

A

G

L

L

F

F

A

V

-

N

G

G

D

S

A

E

L

N

Q

K

V

I

I

L

R

Q

D

N

A

A

G

D

V

E

G

I

H

H

V

V

W

-

S

-

T

T

D

D

C

T

I

V

A

E

H

S

G

K

S

F

N

S

A

D

V

I

S

S

M

V

A

Y

P

Q

Q

E

L

V

A

C

E

N

A

Y

L

I

R

R

P

D

L

I

binding diphosphomethylphosphonic acid adenosyl ester: F32 (= F34), D34 (= D36), E36 (= E38), R91 (= R97), Q92 (= Q98), H93 (≠ D99), Y96 (≠ F102), H124 (= H130), D125 (≠ L131)
binding sulfate ion: R15 (= R17), S58 (≠ P64), S214 (= S225), T215 (≠ S226), G216 (= G227), T218 (= T229)

Sites not aligning to the query:

binding sulfate ion: 7, 11

3mbiD Crystal structure of the phosphoribosylpyrophosphate (prpp) synthetase from thermoplasma volcanium in complex with adp-mg2+ and ribose 5- phosphate (see paper)
25% identity, 95% coverage: 15:296/298 of query aligns to 13:284/285 of 3mbiD

query
sites
3mbiD

A

A

L

A

R

R

L

I

A

A

E

E

A

E

C

L

G

K

L

T

T

E

A

P

A

V

Q

M

I

P

E

D

R

E

H

R

R

R

F
|
F

P

P

D
|
D

D

G

E
|
E

L

L

R

Y

L

L

R

R

L

Y

P

D

L

-

A

-

D

-

G

-

E

E

A

D

I

L

A

T

E

G

Q

H

L

N

V

I

I

F

Y

I

R

I

G

G

L

N

D

T

H

H

P

S

N

D

E

A

K

E

L

V

V

M

E

E

L

M

L

I

L

L

I

T

A

L

G

S

E

A

A

I

R

Q

R

D

L

Y

G

R

A

T

K

K

R

S

L

V

V

N

L

I

V

I

T

A

P

P

Y

Y

L

Y

A

G

Y

Y

M

A

R
|
R

Q
|
Q

D

H

I

Q

A

R

F
x
Y

N

K

P

N

G

G

E

E

V

P

V

I

S

S

Q

S

R

Q

V

I

I

L

G

T

Q

E

L

I

L

Y

A

S

G

S

Q

Y

F

S

D

N

G

S

L

I

I

A

T

T

V

V

D

D

P

I

H
|
H

L

D

H

E

R

K

V

T

A

L

T

S

L

Y

Q

S

E

K

A

-

V

V

P

K

L

F

E

S

D

D

A

-

V

-

T

-

L

L

C

H

A

A

A

N

P

D

A

A

L

I

A

V

R

R

L

Y

I

Y

A

K

E

N

R

V

H

D

P

V

D

D

A

-

L

Y

L

V

I

V

G

S

P

P

D

D

A

D

E

G

A

G

L

L

Q

A

W

R

I

V

E

A

A

D

A

I

A

S

A

A

E

K

H

L

G

G

F

K

A

K

H

H

G

F

V

F

C

I

S

E

K
|
K

V

K

R
|
R

H

I

G

D

D

D

H

R

Q

T

V

V

E

E

I

M

A

K

L

V

P

P

E

N

L

V

Q

D

F

V

A

N

G

G

R

K

H

K

V

L

L

I

L

V

D
|
D

V
x
I

A

I

S
|
S

S
x
T

G
|
G

R

G

T
|
T

L

I

A

A

Q

K

A

S

E

G

K

L

L

L

L

R

A

E

A

K

G

G

A

A

A

S

S

K

V

I

D

Y

V

V

A

S

V

A

T

V

H

H

A

G

L

L

F

F

A

V

-

N

G

G

D

S

A

E

L

N

Q

K

V

I

I

L

R

Q

D

N

A

A

G

D

V

E

G

I

H

H

V

V

W

-

S

-

T

T

D

D

C

T

I

V

A

E

H

S

G

K

S

F

N

S

A

D

V

I

S

S

M

V

A

Y

P

Q

Q

E

L

V

A

C

E

N

A

Y

L

I

R

R

P

D

L

I

binding adenosine-5'-diphosphate: F32 (= F34), D34 (= D36), E36 (= E38), R91 (= R97), Q92 (= Q98), Y96 (≠ F102), H124 (= H130), K184 (= K195), R186 (= R197)
binding 5-O-phosphono-alpha-D-ribofuranose: R91 (= R97), H124 (= H130), K184 (= K195), R186 (= R197), D210 (= D221), D211 (= D222), I212 (≠ V223), S214 (= S225), T215 (≠ S226), G216 (= G227), T218 (= T229)

5t3oA Crystal structure of the phosphorybosylpyrophosphate synthetase ii from thermus thermophilus (see paper)
30% identity, 97% coverage: 5:293/298 of query aligns to 2:293/307 of 5t3oA

query
sites
5t3oA

L

L

Y

I

F

F

D

S

-

G

-

Q

D

S

E

N

R

R

A

P

A

L

A

A

L

Q

R

A

L

I

A

A

E

E

A

A

C

L

G

G

L

L

T

P

A

L

A

G

Q

K

I

S

E

T

R

T

H

L

R

R

F

F

P

A

D

N

D

D

E

N

L

L

R

F

L

V

R

R

L

Y

-

E

P
x
S

L

L

A
x
R

D

E

G

G

E

D

A

V

I

F

A

I

E

V

Q

Q

L

S

V

F

I

V

Y

P

R

P

G

V

L

Q

D

D

H

H

P

-

N

-

E

-

K

-

L

L

V

M

E

E

L

L

L

M

I

M

A

V

G

D

E

A

A

A

R

K

R

G

L

A

G

S

A

A

K

A

R

R

L

V

V

T

L

A

V

V

T

I

P

P

Y

Y

L

F

A

S

Y

Y

M

A

R
|
R

Q
x
S

D

D

I

K

A

K

F
x
D

N

A

P

P

G

R

E

I

V

S

V

I

S

T

Q

A

R

R

V

L

I

I

G

A

Q

D

L

L

-

Q

-

T

A

A

G

G

Q

A

F

-

D

D

G

R

L

V

I

L

T

T

V

M

D

T

P

L

H
|
H

L

S

H

P

R

Q

V

V

A

H

T

G

L

F

Q

F

E

K

A

-

V

I

P

P

L

V

E

D

D

H

A

-

V

-

T

-

L

L

C

S

A

A

A

E

P

P

A

V

L

I

A

A

R

N

L

Y

I

F

A

A

E

T

R

R

-

V

-

D

H

L

P

E

D

N

A

A

L

V

I

V

G

A

P

P

D

D

A

A

E

G

A

D

L

L

Q

K

W

R

I

A

E

S

A

A

A

L

A

A

A

R

E

R

H

L

G

G

F

L

A

P

H

L

G

A

V

F

C

I

S

D

K

K

V

E

R
|
R

H

V

G

S

D

D

H

T

Q

E

V

V

E

R

I

V

A

R

L

M

P

L

E

V

L

G

Q

E

F

V

A

E

G

G

R

K

H

T

V

A

V

L

L

I

L

V

D

D

V

E

A

I

S

S

S

T

G

A

R

G

T

S

L

L

A

V

Q

E

A

A

A

V

E

E

K

A

L

L

L

M

A

Q

A

A

G

G

A

A

A

K

S

E

V

V

D

Y

V

A

A

A

V

A

T

T

H

H

A

G

L

V

F

Y

A

V

G

G

D

P

A

A

L

L

Q

D

V

R

I

I

R

A

D

K

A

S

G

P

V

V

G

K

H

E

V

V

W

A

S

A

T

T

D

D

C

T

I

C

A

P

H

P

G

K

S

E

N

G

-

P

-

K

-

L

-

R

A

T

V

L

S

T

M

V

A

A

P

P

Q

L

L

F

A

A

E

E

A

A

L

I

binding adenosine-5'-diphosphate: R94 (= R97), S95 (≠ Q98), D99 (≠ F102), H128 (= H130), R193 (= R197)
binding sulfate ion: S45 (≠ P44), R47 (≠ A46)

7pn0A Crystal structure of the phosphorybosylpyrophosphate synthetase ii from thermus thermophilus at r32 space group
30% identity, 97% coverage: 5:293/298 of query aligns to 3:294/312 of 7pn0A

query
sites
7pn0A

L

L

Y

I

F

F

D

S

-

G

-

Q

D

S

E

N

R

R

A

P

A

L

A

A

L

Q

R

A

L

I

A

A

E

E

A

A

C

L

G

G

L

L

T

P

A

L

A

G

Q

K

I

S

E

T

R

T

H

L

R

R

F

F

P

A

D

N

D

D

E

N

L

L

R

F

L

V

R

R

L

Y

-

E

P

S

L

L

A

R

D

E

G

G

E

D

A

V

I

F

A

I

E

V

Q

Q

L

S

V

F

I

V

Y

P

R

P

G

V

L

Q

D

D

H

H

P

-

N

-

E

-

K

-

L

L

V

M

E

E

L

L

L

M

I

M

A

V

G

D

E

A

A

A

R

K

R

G

L

A

G

S

A

A

K

A

R

R

L

V

V

T

L

A

V

V

T

I

P

P

Y

Y

L

F

A

S

Y

Y

M

A

R
|
R

Q
x
S

D

D

I

K

A

K

F

D

N

A

P

P

G

R

E

I

V

S

V

I

S

T

Q

A

R

R

V

L

I

I

G

A

Q

D

L

L

-

Q

-

T

A

A

G

G

Q

A

F

-

D

D

G

R

L

V

I

L

T

T

V

M

D

T

P

L

H
|
H

L

S

H

P

R

Q

V

V

A

H

T

G

L

F

Q

F

E

K

A

-

V

I

P

P

L

V

E

D

D

H

A

-

V

-

T

-

L

L

C

S

A

A

A

E

P

P

A

V

L

I

A

A

R

N

L

Y

I

F

A

A

E

T

R

R

-

V

-

D

H

L

P

E

D

N

A

A

L

V

I

V

G

A

P

P

D

D

A

A

E

G

A

D

L

L

Q

K

W

R

I

A

E

S

A

A

A

L

A

A

A

R

E

R

H

L

G

G

F

L

A

P

H

L

G

A

V

F

C

I

S

D

K

K

V

E

R

R

H

V

G

S

D

D

H

T

Q

E

V

V

E

R

I

V

A

R

L

M

P

L

E

V

L

G

Q

E

F

V

A

E

G

G

R

K

H

T

V

A

V

L

L

I

L

V

D

D

V

E

A

I

S

S

S

T

G

A

R

G

T

S

L

L

A

V

Q

E

A

A

A

V

E

E

K

A

L

L

L

M

A

Q

A

A

G

G

A

A

A

K

S

E

V

V

D

Y

V

A

A

A

V

A

T

T

H

H

A

G

L

V

F

Y

A

V

G

G

D

P

A

A

L

L

Q

D

V

R

I

I

R

A

D

K

A

S

G

P

V

V

G

K

H

E

V

V

W

A

S

A

T

T

D

D

C

T

I

C

A

P

H

P

G

K

S

E

N

G

-

P

-

K

-

L

-

R

A

T

V

L

S

T

M

V

A

A

P

P

Q

L

L

F

A

A

E

E

A

A

L

I

binding adenosine-5'-diphosphate: R95 (= R97), S96 (≠ Q98), H129 (= H130)

P60891 Ribose-phosphate pyrophosphokinase 1; PPRibP; Phosphoribosyl pyrophosphate synthase I; PRS-I; EC 2.7.6.1 from Homo sapiens (Human) (see 5 papers)
25% identity, 93% coverage: 17:294/298 of query aligns to 18:301/318 of P60891

query
sites
P60891

R

K

L

I

A

A

E

D

A

R

C

L

G

G

L

L

T

E

A

L

A

G

Q

K

I

V

E

V

R

T

H

K

R

K

F

F

P

S

D

N

D

Q

E

E

L

T

R

C

L

V

R

E

L

I

P

-

L

-

A

-

D

-

G

G

E

E

A

S

I

V

-

R

-

G

A

E

E
x
D

Q

V

L

Y

V

I

I

V

Y

Q

R

S

G

G

L

C

D

G

H

E

P

I

N

N

E

D

K

N

L

L

V

M

E

E

L

L

L

I

I

M

A

I

G

N

E

A

A

C

R

K

R

I

L

A

G

S

A

A

K

S

R

R

L

V

V

T

L

A

V

V

T

I

P

P

Y

C

L

F

A

P

Y

Y

M

A

R

R

Q

Q

D

D

I

K

A

K

F

D

N

K

P

S

G

R

E

A

V

P

V

I

S

S

Q

A

R

K

V

L

I

V

G

A

Q
x
N

L

M

L

L

A

S

-

V

G

A

Q

G

F

A

D

D

G

H

L

I

I

I

T

T

V

M

D

D

P
x
L

H

H

L

A

H
x
S

R

Q

V

I

A

Q

T

G

L

F

Q

F

E

D

A

-

V

I

P

P

L
x
V

E

D

D
x
N

A

-

V

-

T

-

L

L

C
x
Y

A

A

A

E

P

P

A

A

L

V

A

L

R

K

L

W

I

I

A

R

E

E

R

N

H

I

P

S

-

E

-

W

-

R

D

N

A

C

L

T

L

I

I

V

G

S

P

P

D

D

A

A

E

G

A

G

L

A

Q

K

W

R

I

V

E

T

A

S

A

I

A

A

A
x
D

E

R

H

L

G

N

F

V

A

D

H

F

G
x
A

V

L

C

I

S
x
H

K

K

V

E

R

R

H

K

G

K

D

A

H

N

Q

E

V

V

E
x
D

I

R

A

M

L

V

P

L

E

V

L

G

Q

D

F

V

A

K

G

D

R

R

H

V

V

A

V

I

L

L

L
x
V

D

D

V

M

A

A

S

D

S

T

G

C

R

G

T

T

L

I

A

C

Q
x
H

A

A

E

D

K

K

L

L

A

S

A

A

G

G

A

A

A

T

S

R

V

V

D

Y

V

A

A

I

V

L

T

T

H

H

A

G

L

I

F

F

A

S

G

G

D

P

A

A

L

I

Q

S

V

R

I

I

R

N

D

N

A

A

G

C

V

F

G

E

H

A

V

V

W

V

S

V

T

T

D

N

C

T

I

I

-

P

-

Q

-

E

-

D

-

K

-

M

A

K

H

H

G

C

S

S

N

K

-

I

-

Q

A

V

V

I

S

D

M

I

A

S

P

M

Q

I

L

L

A

A

E

E

A

A

L

I

R

R

D52 (≠ E53) to H: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852542
N114 (≠ Q115) to S: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852540
L129 (≠ P129) to I: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852543
S132 (≠ H132) mutation to A: Reduces catalytic activity.; mutation to F: No effect on catalytic activity.
V142 (≠ L143) to L: found in a patient with an intermediate phenotype between ARTS and PRPS1 superactivity; likely pathogenic; normal PRPP synthetase activity in fibroblasts; loss of activity in erythrocytes; dbSNP:rs398122855
N144 (≠ D145) mutation to H: No effect on catalytic activity.
Y146 (≠ C150) mutation to F: No effect on catalytic activity.; mutation to M: Reduces catalytic activity.
D183 (≠ A184) to H: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852541
A190 (≠ G191) to V: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852544
H193 (≠ S194) to Q: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852545
D203 (≠ E204) to H: in a breast cancer sample; somatic mutation
V219 (≠ L220) to G: in a breast cancer sample; somatic mutation
H231 (≠ Q232) to D: in a colorectal cancer sample; somatic mutation

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
16 S → P: found in patients with phosphoribosyl pyrophosphate synthetase I deficiency; likely pathogenic; dbSNP:rs869025594

8dbkB Human prps1 with phosphate, atp, and r5p; hexamer with resolved catalytic loops (see paper)
25% identity, 93% coverage: 17:294/298 of query aligns to 17:300/316 of 8dbkB

query
sites
8dbkB

R

K

L

I

A

A

E

D

A

R

C

L

G

G

L

L

T

E

A

L

A

G

Q

K

I

V

E

V

R

T

H

K

R

K

F
|
F

P

S

D
x
N

D

Q

E
|
E

L

T

R

C

L

V

R

E

L

I

P

-

L

-

A

-

D

-

G

G

E

E

A
x
S

I

V

-
x
R

-

G

A

E

E

D

Q

V

L

Y

V

I

I

V

Y

Q

R

S

G

G

L

C

D

G

H

E

P

I

N

N

E

D

K

N

L

L

V

M

E

E

L

L

L

I

I

M

A

I

G

N

E

A

A

C

R

K

R

I

L

A

G

S

A

A

K

S

R

R

L

V

V

T

L

A

V

V

T

I

P

P

Y

C

L

F

A

P

Y

Y

M

A

R
|
R

Q
|
Q

D

D

I

K

A

K

F

D

N

K

P

S

G

R

E

A

V

P

V

I

S

S

Q

A

R

K

V

L

I

V

G

A

Q

N

L

M

L

L

A

S

-

V

G

A

Q

G

F

A

D

D

G

H

L

I

I

I

T

T

V

M

D

D

P

L

H
|
H

L

A

H

S

R

Q

V

I

A

Q

T

G

L

F

Q

F

E

D

A

-

V

I

P

P

L

V

E

D

D

N

A

-

V

-

T

-

L

L

C

Y

A

A

A

E

P

P

A

A

L

V

A

L

R

K

L

W

I

I

A

R

E

E

R

N

H

I

P

S

-

E

-

W

-

R

D

N

A

C

L

T

L

I

I

V

G

S

P

P

D
|
D

A

A

E
x
G

A

G

L

A

Q

K

W

R

I

V

E

T

A

S

A

I

A

A

A

D

E

R

H

L

G

N

F

V

A

D

H

F

G

A

V

L

C

I

S

H

K
|
K

V

E

R
|
R

H

K

G

K

D

A

H
x
N

Q

E

V

V

E

D

I

R

A

M

L

V

P

L

E

V

L

G

Q

D

F

V

A

K

G

D

R

R

H

V

V

A

V

I

L

L

L

V

D
|
D

V

M

A

A

S
x
D

S
x
T

G
x
C

R
x
G

T
|
T

L

I

A

C

Q

H

A

A

E

D

K

K

L

L

A

S

A

A

G

G

A

A

A

T

S

R

V

V

D

Y

V

A

A

I

V

L

T

T

H

H

A

G

L

I

F

F

A

S

G

G

D

P

A

A

L

I

Q

S

V

R

I

I

R

N

D

N

A

A

G

C

V

F

G

E

H

A

V

V

W

V

S

V

T

T

D

N

C

T

I

I

-

P

-

Q

-

E

-

D

-

K

-

M

A

K

H

H

G

C

S

S

N

K

-

I

-

Q

A

V

V

I

S

D

M

I

A

S

P

M

Q

I

L

L

A

A

E

E

A

A

L

I

R

R

binding adenosine monophosphate: R95 (= R97), Q96 (= Q98), N199 (≠ H201)
binding adenosine-5'-triphosphate: F34 (= F34), N36 (≠ D36), E38 (= E38)
binding phosphate ion: S46 (≠ A50), R48 (vs. gap)
binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: H129 (= H130), D170 (= D172), G172 (≠ E174), K193 (= K195), R195 (= R197), D219 (= D221), D220 (= D222), D223 (≠ S225), T224 (≠ S226), C225 (≠ G227), G226 (≠ R228), T227 (= T229)

8dbeA Human prps1 with adp; hexamer (see paper)
25% identity, 93% coverage: 17:294/298 of query aligns to 17:300/316 of 8dbeA

query
sites
8dbeA

R

K

L

I

A

A

E

D

A

R

C

L

G

G

L

L

T

E

A

L

A

G

Q

K

I

V

E

V

R

T

H

K

R

K

F
|
F

P

S

D
x
N

D

Q

E
|
E

L

T

R

C

L

V

R

E

L

I

P

-

L

-

A

-

D

-

G

G

E

E

A

S

I

V

-

R

-

G

A

E

E

D

Q

V

L

Y

V

I

I

V

Y

Q

R

S

G

G

L

C

D

G

H

E

P

I

N

N

E

D

K

N

L

L

V

M

E

E

L

L

L

I

I

M

A

I

G

N

E

A

A

C

R

K

R

I

L

A

G

S

A

A

K

S

R

R

L

V

V

T

L

A

V

V

T

I

P

P

Y

C

L

F

A

P

Y

Y

M

A

R
|
R

Q
|
Q

D

D

I
x
K

A
x
K

F
x
D

N

K

P
x
S

G
x
R

E

A

V

P

V

I

S

S

Q

A

R

K

V

L

I

V

G

A

Q

N

L

M

L

L

A

S

-

V

G

A

Q

G

F

A

D

D

G

H

L

I

I

I

T

T

V

M

D

D

P

L

H
|
H

L

A

H

S

R

Q

V

I

A

Q

T

G

L

F

Q

F

E

D

A

-

V

I

P

P

L

V

E
x
D

D

N

A

-

V

-

T

-

L

L

C
x
Y

A

A

A

E

P

P

A

A

L

V

A

L

R

K

L

W

I

I

A

R

E

E

R

N

H

I

P

S

-

E

-

W

-

R

D

N

A

C

L

T

L

I

I

V

G

S

P

P

D
|
D

A

A

E

G

A

G

L

A

Q

K

W

R

I

V

E

T

A

S

A

I

A

A

A

D

E

R

H

L

G

N

F

V

A

D

H

F

G

A

V

L

C

I

S

H

K

K

V

E

R

R

H

K

G

K

D

A

H

N

Q

E

V

V

E

D

I

R

A

M

L

V

P

L

E

V

L

G

Q

D

F

V

A

K

G

D

R

R

H

V

V

A

V

I

L

L

L

V

D
|
D

V

M

A

A

S
x
D

S
x
T

G

C

R
x
G

T
|
T

L

I

A

C

Q

H

A

A

E

D

K

K

L

L

A

S

A

A

G

G

A

A

A

T

S

R

V

V

D

Y

V

A

A

I

V

L

T

T

H

H

A

G

L

I

F

F

A

S

G

G

D

P

A

A

L

I

Q

S

V

R

I

I

R

N

D

N

A

A

G

C

V

F

G

E

H

A

V

V

W

V

S

V

T

T

D

N

C

T

I

I

-

P

-

Q

-

E

-

D

-

K

-

M

A

K

H

H

G

C

S

S

N

K

-

I

-

Q

A

V

V

I

S

D

M

I

A

S

P

M

Q

I

L

L

A

A

E

E

A

A

L

I

R

R

binding adenosine-5'-diphosphate: F34 (= F34), N36 (≠ D36), E38 (= E38), R95 (= R97), Q96 (= Q98), K98 (≠ I100), K99 (≠ A101), D100 (≠ F102), S102 (≠ P104), R103 (≠ G105), H129 (= H130), D142 (≠ E144), Y145 (≠ C150)
binding 5-O-phosphono-alpha-D-ribofuranose: H129 (= H130), D170 (= D172), D219 (= D221), D220 (= D222), D223 (≠ S225), T224 (≠ S226), G226 (≠ R228), T227 (= T229)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 307, 308, 309, 312

7yk1A Structural basis of human prps2 filaments (see paper)
26% identity, 93% coverage: 17:294/298 of query aligns to 17:291/306 of 7yk1A

query
sites
7yk1A

R

R

L

V

A

A

E

D

A

R

C

L

G

G

L

L

T

E

A

L

A

G

Q

K

I

V

E

V

R

T

H

K

R

K

F
|
F

P

S

D
x
N

D

Q

E
|
E

L

T

R

S

L

V

R

E

L

I

P

-

L

-

A

-

D

-

G

G

E

E

A
x
S

I

V

-
x
R

-

G

A

E

E

D

Q

V

L

Y

V

I

I

I

Y

Q

R

S

G

G

L

C

D

G

H

E

P

I

N

N

E

D

K

N

L

L

V

M

E

E

L

L

L

I

I

M

A

I

G

N

E

A

A

C

R

K

R

I

L

A

G

S

A

S

K

S

R

R

L

V

V

T

L

A

V

V

T

I

P

P

Y

C

L

F

A

P

Y

Y

M

A

R
|
R

Q

Q

D

D

I

K

A
x
K

F
x
D

N
x
K

P
x
S

G
x
R

E

A

V

P

V

I

S

S

Q

A

R

K

V

L

I

V

G

A

Q

N

L

M

L

L

A

S

-

V

G

A

Q

G

F

A

D

D

G

H

L

I

I

I

T

T

V

M

D

D

P

L

H
|
H

L

A

H

S

R

Q

V

I

A

Q

T

G

L

F

Q

F

E

D

A

-

V

I

P

P

L

V

E
x
D

D

N

A

-

V

-

T

-

L

L

C

Y

A

A

A

E

P

P

A

A

L

V

A

L

R

Q

L

W

I

I

A

R

E

E

R

N

-

I

-

A

-

E

H

W

P

K

D

N

A

C

L

I

I

V

G

S

P

P

D

D

A

A

E

G

A

G

L

A

Q

K

W

R

I

V

E

T

A

S

A

I

A

A

A

D

E

R

H

L

G

-

F

-

A

-

H

-

G

-

V

-

C

-

S

-

K

-

V

-

R

-

H

-

G

-

D

-

H

-

Q

N

V

V

E

E

I

F

A

A

L

L

-

I

-

H

P

K

E

E

L

M

Q

V

F

L

A

V

G

G

-

D

-

V

-

K

-

D

R

R

H

V

V

A

V

I

L

L

L

V

D

D

V

M

A

A

S
x
D

S

T

G
x
C

R

G

T
|
T

L

I

A

C

Q

H

A

A

E

D

K

K

L

L

A

S

A

A

G

G

A

A

A

T

S

K

V

V

D

Y

V

A

A

I

V

L

T

T

H

H

A

G

L

I

F

F

A

S

G

G

D

P

A

A

L

I

Q

S

V

R

I

I

R

N

D

N

A

A

G

A

V

F

G

E

H

A

V

V

W

V

S

V

T

T

D

N

C

T

I

I

-

P

-

Q

-

E

-

D

-

K

-

M

A

K

H

H

G

C

S

T

N

K

-

I

-

Q

A

V

V

I

S

D

M

I

A

S

P

M

Q

I

L

L

A

A

E

E

A

A

L

I

R

R

binding adenosine-5'-diphosphate: F34 (= F34), N36 (≠ D36), E38 (= E38), S46 (≠ A50), R48 (vs. gap), R95 (= R97), K99 (≠ A101), D100 (≠ F102), K101 (≠ N103), S102 (≠ P104), R103 (≠ G105), H129 (= H130), D142 (≠ E144)
binding phosphate ion: D214 (≠ S225), C216 (≠ G227), T218 (= T229)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 298, 300, 303

2hcrA Crystal structure of human phosphoribosyl pyrophosphate synthetase 1 in complex with amp(atp), cadmium and sulfate ion (see paper)
26% identity, 93% coverage: 17:294/298 of query aligns to 16:293/305 of 2hcrA

query
sites
2hcrA

R

K

L

I

A

A

E

D

A

R

C

L

G

G

L

L

T

E

A

L

A

G

Q

K

I

V

E

V

R

T

H

K

R

K

F

F

P

S

D

N

D

Q

E

E

L

T

R

C

L

V

R

E

L

I

P

-

L

-

A

-

D

-

G

G

E

E

A

S

I

V

-

R

-

G

A

E

E

D

Q

V

L

Y

V

I

I

V

Y

Q

R

S

G

G

L

C

D

G

H

E

P

I

N

N

E

D

K

N

L

L

V

M

E

E

L

L

L

I

I

M

A

I

G

N

E

A

A

C

R

K

R

I

L

A

G

S

A

A

K

S

R

R

L

V

V

T

L

A

V

V

T

I

P

P

Y

C

L

F

A

P

Y

Y

M

A

R
|
R

Q
|
Q

D

D

I
x
K

A

K

F

D

N

K

P

S

G

R

E

A

V

P

V

I

S

S

Q

A

R

K

V

L

I

V

G

A

Q

N

L

M

L

L

A

S

-

V

G

A

Q

G

F

A

D

D

G

H

L

I

I

I

T

T

V

M

D

D

P

L

H
|
H

L

A

H

S

R

Q

V

I

A

Q

T

G

L

F

Q

F

E

D

A

-

V

I

P

P

L

V

E

D

D

N

A

-

V

-

T

-

L

L

C

Y

A

A

A

E

P

P

A

A

L

V

A

L

R

K

L

W

I

I

A

R

E

E

R

N

H

I

P

S

-

E

-

W

-

R

D

N

A

C

L

T

L

I

I

V

G

S

P

P

D

D

A

A

E

G

A

G

L

A

Q

K

W

R

I

V

E

T

A

S

A

I

A

A

A

D

E

R

H

L

G

N

F

V

A

D

H

F

G

A

V

L

C

I

S

H

K

K

V

E

R

R

H

-

G

-

D

D

H

R

Q

M

V

V

E

L

I

V

A

G

L

-

P

-

E

-

L

-

Q

D

F

V

A

K

G

D

R

R

H

V

V

A

V

I

L

L

L

V

D

D

V

M

A

A

S

D

S

T

G

C

R

G

T

T

L

I

A

C

Q

H

A

A

E

D

K

K

L

L

A

S

A

A

G

G

A

A

A

T

S

R

V

V

D

Y

V

A

A

I

V

L

T

T

H

H

A

G

L

I

F

F

A

S

G

G

D

P

A

A

L

I

Q

S

V

R

I

I

R

N

D

N

A

A

G

C

V

F

G

E

H

A

V

V

W

V

S

V

T

T

D

N

C

T

I

I

-

P

-

Q

-

E

-

D

-

K

-

M

A

K

H

H

G

C

S

S

N

K

-

I

-

Q

A

V

V

I

S

D

M

I

A

S

P

M

Q

I

L

L

A

A

E

E

A

A

L

I

R

R

binding adenosine monophosphate: R94 (= R97), Q95 (= Q98), K97 (≠ I100), H128 (= H130)

6nfeA Crystal structure of ribose-phosphate pyrophosphokinase from legionella pneumophila with bound amp, adp, and ribose-5-phosphate
27% identity, 84% coverage: 45:294/298 of query aligns to 34:294/298 of 6nfeA

query
sites
6nfeA

L
x
F

A

S

D
|
D

G

G

E
|
E

A

T

I

M

A

V

E

E

Q

I

L

L

V

E

I

N

Y

V

R

R

G

G

L

K

D

D

-

V

-

F

-

V

-

L

-

Q

-

S

-

T

-

C

-

A

H

P

P

A

N

N

E

N

K

N

L

L

V

M

E

E

L

L

L

I

I

M

A

A

G

D

E

A

A

L

R

R

L

S

G

S

A

A

K

G

R

R

L

I

V

T

L

A

V

V

T

V

P

P

Y

Y

L

F

A

G

Y

Y

M

A

R
|
R

Q
|
Q

D

D

I
x
R

A

R

F
x
V

N

R

P

S

G

A

E

R

V

V

-

P

V

I

S

T

Q

A

R

K

V

V

I

V

G

A

Q

D

L

M

A

A

G

S

-

V

Q

G

F

I

D

C

G

R

L

V

I

L

T

T

V

V

D

D

P

L

H
|
H

L

A

H

D

R

Q

V

I

A

Q

T

G

L

F

Q

F

E

Y

A

-

V

M

P

P

L

V

E

D

D

N

A

-

V

-

T

-

L

V

C
x
Y

A

S

A

T

P

P

A

V

L

L

A

L

R

E

L

D

I

I

A

T

E

K

R

Q

H

K

P

L

D

N

A

N

L

I

L

M

I

I

-

V

G

S

P

P

D

D

A

V

E

G

A

G

L

V

Q

V

W
x
R

I

A

E

R

A
|
A

A

V

A

A

A
x
K

E

R

H

L

G

N

F

D

A

A

H

E

-

L

G

S

V

I

C

I

S

D

K

K

V

R

R

R

H

E

G

V

D

M

H

H

Q

I

V

I

E

G

I

-

A

-

L

-

P

-

E

-

L

-

Q

E

F

P

A

A

G

N

R

K

H

N

V

C

V

I

L

I

L

V

D
|
D

V
x
I

A

V

S
x
D

S
x
T

G
x
A

R

G

T
|
T

L

L

A

C

Q

T

A

A

E

H

K

E

L

L

L

K

A

K

A

N

G

G

A

A

A

K

S

S

V

V

D

R

V

A

A

Y

V

I

T

T

H

H

A

P

L

V

F

L

A

S

G

G

D

P

A

A

L

V

Q

N

V

N

I

I

R

K

D

H

A

S

G

G

V

L

G

D

H

E

V

V

W

V

S

V

T

T

D

D

C

T

I

I

A

P

H

L

G

S

S

A

N

E

A

A

-

Q

-

N

-

C

-

E

-

K

-

I

-

R

-

V

V

V

S

S

M

L

A

A

P

D

Q

M

L

L

A

A

E

Q

A

A

L

I

R

K

binding adenosine-5'-diphosphate: F34 (≠ L45), D36 (= D47), E38 (= E49), R95 (= R97), Q96 (= Q98), H130 (= H130)
binding adenosine monophosphate: R98 (≠ I100), V100 (≠ F102), Y146 (≠ C150), R175 (≠ W178), A178 (= A181), K181 (≠ A184)
binding 5-O-phosphono-alpha-D-ribofuranose: H130 (= H130), D213 (= D221), D214 (= D222), I215 (≠ V223), D217 (≠ S225), T218 (≠ S226), A219 (≠ G227), T221 (= T229)

6nfeB Crystal structure of ribose-phosphate pyrophosphokinase from legionella pneumophila with bound amp, adp, and ribose-5-phosphate
27% identity, 84% coverage: 45:294/298 of query aligns to 34:295/299 of 6nfeB

query
sites
6nfeB

L
x
F

A

S

D
|
D

G

G

E
|
E

A

T

I

M

A

V

E

E

Q

I

L

L

V

E

I

N

Y

V

R

R

G

G

L

K

D

D

-

V

-

F

-

V

-

L

-

Q

-

S

-

T

-

C

-

A

H

P

P

A

N

N

E

N

K

N

L

L

V

M

E

E

L

L

L

I

I

M

A

A

G

D

E

A

A

L

R

R

L

S

G

S

A

A

K

G

R

R

L

I

V

T

L

A

V

V

T

V

P

P

Y

Y

L

F

A

G

Y

Y

M

A

R
|
R

Q
|
Q

D

D

I

R

A

R

F

V

N

R

P

S

G

A

E

R

V

V

-

P

V

I

S

T

Q

A

R

K

V

V

I

V

G

A

Q

D

L

M

A

A

G

S

-

V

Q

G

F

I

D

C

G

R

L

V

I

L

T

T

V

V

D

D

P

L

H
|
H

L

A

H

D

R

Q

V

I

A

Q

T

G

L

F

Q

F

E

Y

A

-

V

M

P

P

L

V

E

D

D

N

A

-

V

-

T

-

L

V

C

Y

A

S

A

T

P

P

A

V

L

L

A

L

R

E

L

D

I

I

A

T

E

K

R

Q

H

K

P

L

D

N

A

N

L

I

L

M

I

I

-

V

G

S

P

P

D

D

A

V

E

G

A

G

L

V

Q

V

W

R

I

A

E

R

A

A

A

V

A

A

A

K

E

R

H

L

G

N

F

D

A

A

H

E

G

L

V

S

C

I

S

I

K

D

V

K

R

R

H

R

G

S

D

E

H

V

Q

M

V

H

E

I

I

I

A

G

L

E

P

P

E

-

L

-

Q

-

F

-

A

A

G

N

R

K

H

N

V

C

V

I

L

I

L

V

D
|
D

V
x
I

A

V

S
x
D

S
x
T

G
x
A

R

G

T
|
T

L

L

A

C

Q

T

A

A

E

H

K

E

L

L

L

K

A

K

A

N

G

G

A

A

A

K

S

S

V

V

D

R

V

A

A

Y

V

I

T

T

H

H

A

P

L

V

F

L

A

S

G

G

D

P

A

A

L

V

Q

N

V

N

I

I

R

K

D

H

A

S

G

G

V

L

G

D

H

E

V

V

W

V

S

V

T

T

D

D

C

T

I

I

A

P

H

L

G

S

S

A

N

E

A

A

-

Q

-

N

-

C

-

E

-

K

-

I

-

R

-

V

V

V

S

S

M

L

A

A

P

D

Q

M

L

L

A

A

E

Q

A

A

L

I

R

K

binding adenosine-5'-diphosphate: F34 (≠ L45), D36 (= D47), E38 (= E49), R95 (= R97), Q96 (= Q98), H130 (= H130)
binding 5-O-phosphono-alpha-D-ribofuranose: H130 (= H130), D214 (= D221), D215 (= D222), I216 (≠ V223), D218 (≠ S225), T219 (≠ S226), A220 (≠ G227), T222 (= T229)

8dbgA Human prps1 with phosphate and atp; hexamer (see paper)
25% identity, 93% coverage: 17:294/298 of query aligns to 17:293/309 of 8dbgA

query
sites
8dbgA

R

K

L

I

A

A

E

D

A

R

C

L

G

G

L

L

T

E

A

L

A

G

Q

K

I

V

E

V

R

T

H

K

R

K

F
|
F

P

S

D
x
N

D

Q

E
|
E

L

T

R

C

L

V

R

E

L

I

P

-

L

-

A

-

D

-

G

G

E

E

A
x
S

I

V

-
x
R

-

G

A

E

E

D

Q

V

L

Y

V

I

I

V

Y

Q

R

S

G

G

L

C

D

G

H

E

P

I

N

N

E

D

K

N

L

L

V

M

E

E

L

L

L

I

I

M

A

I

G

N

E

A

A

C

R

K

R

I

L

A

G

S

A

A

K

S

R

R

L

V

V

T

L

A

V

V

T

I

P

P

Y

C

L

F

A

P

Y

Y

M

A

R
|
R

Q
|
Q

D

D

I
x
K

A

K

F

D

N

K

P

S

G

R

E

A

V

P

V

I

S

S

Q

A

R

K

V

L

I

V

G

A

Q

N

L

M

L

L

A

S

-

V

G

A

Q

G

F

A

D

D

G

H

L

I

I

I

T

T

V

M

D

D

P

L

H
|
H

L

A

H

S

R

Q

V

I

A

Q

T

G

L

F

Q

F

E

D

A

-

V

I

P

P

L

V

E

D

D

N

A

-

V

-

T

-

L

L

C

Y

A

A

A

E

P

P

A

A

L

V

A

L

R

K

L

W

I

I

A

R

E

E

R

N

H

I

P

S

-

E

-

W

-

R

D

N

A

C

L

T

L

I

I

V

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S

P

P

D

D

A

A

E

G

A

G

L

A

Q

K

W

R

I

V

E

T

A

S

A

I

A

A

-

D

-

R

-

L

-

N

A

V

E

D

H

F

G

A

F

L

A

I

H

H

G

K

V

E

C

D

S

R

K

M

V

V

R

L

H

V

G

G

D

D

H

V

Q

K

V

D

E

R

I

V

A

A

L

-

P

-

E

-

L

-

Q

-

F

-

A

-

G

-

R

-

H

-

V

-

V

I

L

L

L

V

D

D

V

M

A

A

S
x
D

S
x
T

G
x
C

R

G

T
|
T

L

I

A

C

Q

H

A

A

E

D

K

K

L

L

A

S

A

A

G

G

A

A

A

T

S

R

V

V

D

Y

V

A

A

I

V

L

T

T

H

H

A

G

L

I

F

F

A

S

G

G

D

P

A

A

L

I

Q

S

V

R

I

I

R

N

D

N

A

A

G

C

V

F

G

E

H

A

V

V

W

V

S

V

T

T

D

N

C

T

I

I

-

P

-

Q

-

E

-

D

-

K

-

M

A

K

H

H

G

C

S

S

N

K

-

I

-

Q

A

V

V

I

S

D

M

I

A

S

P

M

Q

I

L

L

A

A

E

E

A

A

L

I

R

R

binding adenosine-5'-triphosphate: F34 (= F34), N36 (≠ D36), E38 (= E38), R95 (= R97), Q96 (= Q98), K98 (≠ I100), H129 (= H130)
binding phosphate ion: S46 (≠ A50), R48 (vs. gap), D216 (≠ S225), T217 (≠ S226), C218 (≠ G227), T220 (= T229)

O94413 Ribose-phosphate pyrophosphokinase 2; Phosphoribosyl pyrophosphate synthase 2; EC 2.7.6.1 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
24% identity, 94% coverage: 15:294/298 of query aligns to 18:304/321 of O94413

query
sites
O94413

A

A

L

E

R

K

L

V

A

A

E

R

A

R

C

I

G

G

L

L

T

S

A

L

A

G

Q

K

I

V

E

A

R

V

H

V

R

Q

F

Y

P

S

D

N

D

R

E

E

L

T

R

S

L

V

R

T

L

I

P

-

L

-

A

-

D

-

G

G

E

E

A

S

I

V

A

R

E

D

Q

E

-

D

-

V

L

F

V

I

I

L

Y

Q

R

T

G

G

L

C

D

G

H

S

P

I

N

N

E

D

K

H

L

L

V

M

E

E

L

L

L

I

I

M

A

I

G

N

E

A

A

C

R

R

R

S

L

A

G

S

A

A

K

R

R

R

L

I

V

T

L

A

V

I

T

I

P

P

Y

C

L

F

A

P

Y

Y

M

A

R

R

Q

Q

D

D

I

K

A

K

F

D

N

K

P

S

G

R

E

A

V

P

V

I

S

T

Q

A

R

R

V

L

I

V

G

A

Q

N

L

M

L

L

-

Q

A

T

G

A

Q

G

F

C

D

N

G

H

L

I

I

I

T

T

V

M

D

D

P

L

H

H

L

A

H

S

R

Q

V

I

A

Q

T

G

L

F

Q

F

E

N

A

-

V

V

P

P

L

V

E

D

D

N

A

-

V

-

T

-

L

L

C

Y

A

A

A

E

P

P

A

S

L

V

A

L

R

R

L

Y

I

I

A

R

E

E

R

N

-

I

-

D

-

T

-

V

H

N

P

P

D

-

A

T

L

V

L

I

I

V

G
x
S

P

P

D

D

A

A

E

G

A

G

L

A

Q

K

W

R

I

A

E

T

A

A

A

L

A

A

A

D

E

R

H

L

G

D

F

L

A

D

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F

G

A

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L

C

I

S

H

K

K

V

E

R

R

H

Q

G

K

D

A

H

N

Q

E

V

V

E

S

I

R

A

M

L

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P

L

E

V

L

G

Q

D

F

V

A

R

G

D

R

K

H

L

V

A

V

I

L

L

L

V

D

D

V

M

A

A

S

D

S

T

G

C

R

G

T

T

L

L

A

G

Q

L

A

A

E

K

K

T

L

L

L

K

A

D

A

N

G

G

A

A

A

K

S

A

V

V

D

Y

V

A

A

I

V

V

T

T

H

H

A

G

L

I

F

L

A

S

G

G

D

K

A

A

L

I

Q

K

V

V

I

I

R

N

D

E

A

S

G

A

V

L

G

E

H

K

V

V

W

I

S

V

T

T

D

N

C

T

I

I

A

P

H

H

G

D

S

D

N

K

-

R

-

S

-

L

-

C

-

S

-

K

-

I

-

E

A

T

V

I

S

D

M

I

A

S

P

G

Q

V

L

L

A

A

E

E

A

C

L

I

R

R

S172 (≠ G170) modified: Phosphoserine

P0A717 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Escherichia coli (strain K12) (see 4 papers)
26% identity, 88% coverage: 33:294/298 of query aligns to 34:301/315 of P0A717

query
sites
P0A717

R

R

F

F

P

S

D

D

D

G

E

E

L

V

R

S

L

V

R

Q

L

I

P

-

L

-

A

-

D

-

G

N

E

E

A

N

I

V

-

R

-

G

A

G

E

D

Q

I

L

F

V

I

I

I

Y

Q

R

S

G

T

L

C

D

A

H

P

P

T

N

N

E

D

K

N

L

L

V

M

E

E

L

L

L

V

I

M

A

V

G

D

E

A

A

L

R

R

L

A

G

S

A

A

K

G

R

R

L

I

V

T

L

A

V

V

T

I

P

P

Y

Y

L

F

A

G

Y

Y

M

A

R

R

Q

Q

D

D

I

R

A

R

F

V

N

R

P

S

G

A

E

R

V

V

-

P

V

I

S

T

Q

A

R

K

V

V

I

V

G

A

Q

D

L

F

L

L

A

S

G

S

-

V

Q

G

F

V

D

D

G

R

L

V

I

L

T

T

V

V

D
|
D

P

L

H

H

L

A

H

E

R

Q

V

I

A

Q

T

G

L

F

Q

F

E

D

A

-

V

V

P

P

L

V

E

D

D

N

A

-

V

-

T

-

L

V

C

F

A

G

A

S

P

P

A

I

L

L

A

L

R

E

L

D

I

M

A

L

E

Q

R

L

H

N

P

L

D

D

A

N

-

P

L

I

L

V

I

V

G

S

P

P

D

D

-

I

-

G

-

V

-

R

-

A

-

R

-

A

-

I

A

A

E

K

A

L

L

L

Q

N

W

D

I

T

E

D

A

M

A

A

A

I

E

D

H

K

G

R

F

R

A

P

H

R

G

A

V

N

C

V

S

S

K

Q

V

V

R

M

H

H

-

I

G

G

D

D

H

-

Q

-

V

-

E

-

I

-

A

-

L

-

P

-

E

-

L

-

Q

-

F

V

A

A

G

G

R

R

H

D

V

C

V

V

L

L

L

V

D
|
D

V

M

A

I

S
x
D

S

T

G

G

R

G

T

T

L

L

A

C

Q

K

A

A

E

E

K

A

L

L

L

K

A

E

A

R

G

G

A

A

A

K

S

R

V

V

D

F

V

A

A

Y

V

A

T

T

H

H

A

P

L

I

F

F

A

S

G

G

D

N

A

A

L

A

Q

N

V

N

I

L

R

R

D

N

A

S

G

V

V

I

G

D

H

E

V

V

W

V

S

V

T

C

D

D

C

T

I

I

A

P

H

L

G

S

S

D

N

E

-

I

-

K

-

S

-

L

-

P

-

N

-

V

-

R

A

T

V

L

S

T

M

L

A

S

P

G

Q

M

L

L

A

A

E

E

A

A

L

I

R

R

D129 (= D128) to A: in mutant PRSA1; alters the binding of divalent cations, especially magnesium. Little alteration in the affinity for ribose 5-phosphate and 27-fold decrease of the affinity for ATP. Absence of inhibition by AMP
D220 (= D221) mutation to E: 4-fold decrease in the affinity binding for Rib-5-P in the presence of magnesium ions. In the presence of cobalt ions, it shows a 15-fold decrease in the affinity binding for Rib-5-P.; mutation to F: With magnesium or manganese ions, the affinity binding values for ATP and Rib-5-P are comparable to those of the wild-type.
D221 (= D222) mutation to A: The affinity binding for ATP is comparable to those of the wild-type, apart from a slight decrease in the presence of manganese ions. The affinity binding for Rib-5-P is greatly decreased in the presence of both manganese and cobalt ions but only about 2-fold in the presence of magnesium ions.
D224 (≠ S225) mutation to A: With magnesium or manganese ions, the affinity binding values for ATP and Rib-5-P are comparable to those of the wild-type.; mutation to S: With magnesium or manganese ions, the affinity binding values for ATP and Rib-5-P are comparable to those of the wild-type.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

Query Sequence

>GFF3459 FitnessBrowser__psRCH2:GFF3459
MQSQLLYFDDERAAALRLAEACGLTAAQIERHRFPDDELRLRLPLADGEAIAEQLVIYRG
LDHPNEKLVELLLIAGEARRLGAKRLVLVTPYLAYMRQDIAFNPGEVVSQRVIGQLLAGQ
FDGLITVDPHLHRVATLQEAVPLEDAVTLCAAPALARLIAERHPDALLIGPDAEALQWIE
AAAAEHGFAHGVCSKVRHGDHQVEIALPELQFAGRHVVLLDDVASSGRTLAQAAEKLLAA
GAASVDVAVTHALFAGDALQVIRDAGVGHVWSTDCIAHGSNAVSMAPQLAEALRPLLR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory