SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF3462 FitnessBrowser__Marino:GFF3462 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P25526 Succinate-semialdehyde dehydrogenase [NADP(+)] GabD; SSDH; Glutarate-semialdehyde dehydrogenase; EC 1.2.1.79; EC 1.2.1.- from Escherichia coli (strain K12) (see paper)
45% identity, 97% coverage: 7:475/483 of query aligns to 8:474/482 of P25526

query
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P25526

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WN 156:157 (= WN 155:156) binding
KPAS 180:183 (= KPAS 179:182) binding
GS 233:234 (= GS 233:234) binding
L256 (= L256) binding
E386 (= E387) binding

3jz4A Crystal structure of e. Coli NADP dependent enzyme (see paper)
45% identity, 97% coverage: 7:475/483 of query aligns to 7:473/481 of 3jz4A

query
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3jz4A

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active site: N156 (= N156), K179 (= K179), E254 (= E255), C288 (= C289), E385 (= E387), E462 (= E464)
binding nadp nicotinamide-adenine-dinucleotide phosphate: I152 (= I152), P154 (= P154), W155 (= W155), N156 (= N156), K179 (= K179), A181 (= A181), S182 (= S182), S211 (≠ K212), A212 (= A213), G213 (≠ S214), G216 (= G217), F230 (= F231), T231 (= T232), G232 (= G233), S233 (= S234), I236 (≠ V237), E254 (= E255), L255 (= L256), C288 (= C289), K338 (= K339), E385 (= E387), F387 (= F389)

P51649 Succinate-semialdehyde dehydrogenase, mitochondrial; Aldehyde dehydrogenase family 5 member A1; NAD(+)-dependent succinic semialdehyde dehydrogenase; EC 1.2.1.24 from Homo sapiens (Human) (see 5 papers)
41% identity, 99% coverage: 4:482/483 of query aligns to 55:533/535 of P51649

query
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P51649

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G

G

D
x
N

G

G

I

F

N

E

G

E

D

G

V

T

D

T

L

Q

G

G

P
|
P

L

L

I

I

N

N

Q

E

A

K

G

A

Y

V

D

E

K

K

V

V

K

E

R

K

H

Q

V

V

Q

N

D

D

A

A

L

V

E

S

K

K

G

G

A

A

T

T

L
x
V

V

V

A

T

G
|
G

K

G

K

K

P

R

E

H

D

Q

L

L

G

G

N

K

D

N

L

-

F

F

P

E

P

P

T

T

V

L

H

C

G

N

V

V

N

T

R

Q

D

D

M

M

C

L

C

C

Y

T

Q

H

E

E

T

T

F

F

G

G

P

P

L

L

V

A

P

P

M

V

A

I

L

K

F

F

R

D

T

T

E

E

V

A

I

I

E

A

A

I

G

A

N

N

D

A

T

A

E

D

F

V

G

G

L

L

A

A

S

G

Y

Y

V

F

F

Y

T

S

N

Q

D

D

A

P

E

A

R

Q

A

I

Q

W

R

R

V

V

A

A

A

E

G

Q

L

L

R

E

F

V

G

G

H

M

C

V

G

G

W

V

N

N

T

E

G

G

T

L

G

I

P
x
S

T

S

P

V

E

E

A

C

P

P

F

F

G

G

M

V

K

K

A

Q

S

S

G

G

I

L

G

G

R

R

E

E

G

G

G

S

L

K

E

Y

G

G

L

I

F

D

E

E

F

Y

V

L

E

E

A

L

Q

K

T

Y

V

V

P

C

R

Y

G
|
G

C93 (≠ M44) to F: in SSADHD; 3% of activity; dbSNP:rs765561257
G176 (≠ A127) to R: in SSADHD; <1% of activity; dbSNP:rs72552281
H180 (≠ P131) to Y: 83% of activity; dbSNP:rs2760118
P182 (≠ K133) to L: 48% of activity; dbSNP:rs3765310
R213 (≠ K164) binding ; mutation to A: Reduces catalytic activity to less than 15% of wild-type.
C223 (= C174) to Y: in SSADHD; 5% of activity; dbSNP:rs72552282
KPAE 228:231 (≠ KPAS 179:182) binding
T233 (= T184) to M: in SSADHD; 4% of activity; dbSNP:rs1326526453
A237 (≠ M188) to S: 65% of activity; dbSNP:rs62621664
N255 (= N207) to S: in SSADHD; 17% of activity; dbSNP:rs145087265
G268 (= G217) to E: in SSADHD; <1% of activity; dbSNP:rs375628463
GSTTTG 284:289 (≠ GSTEVG 233:238) binding
R334 (= R283) binding ; mutation to A: Reduces catalytic activity to less than 15% of wild-type.
N335 (≠ G284) to K: in SSADHD; 1% of activity; dbSNP:rs72552283
C340 (= C289) modified: Disulfide link with 342, In inhibited form
C342 (= C291) modified: Disulfide link with 340, In inhibited form; mutation to A: Loss of regulation by redox state.
N372 (≠ D320) natural variant: N -> S
P382 (= P330) to L: in SSADHD; 2% of activity
V406 (≠ L354) to I: in dbSNP:rs143741652
G409 (= G357) to D: in SSADHD; <1% of activity; dbSNP:rs118203984
S498 (≠ P447) binding ; mutation to A: Reduces catalytic activity to less than 15% of wild-type.
G533 (= G482) to R: in SSADHD; <1% of activity; dbSNP:rs72552284

Sites not aligning to the query:

36 G → R: no effect on succinate-semialdehyde dehydrogenase activity; dbSNP:rs4646832

2w8rA The crystal structure of human ssadh in complex with NAD+ (see paper)
41% identity, 99% coverage: 4:482/483 of query aligns to 5:483/485 of 2w8rA

query
sites
2w8rA

S

S

P

A

L

A

L

L

E

L

K

R

L

T

T

D

G

S

Y

F

I

V

G

G

R

R

W

W

T

L

D

P

N

-

E

-

H

A

G

A

N

A

T

T

F

F

D

P

V

V

Y

Q

N

D

P

P

A

A

T

S

G

G

K

A

V

A

I

L

A

G

Q

M

V

V

A

A

S

D

M

C

S

G

E

V

D

R

E

E

V

A

N

R

A

A

V

V

A

R

S

A

G

A

K

Y

S

E

A

A

L

F

R

C

L

R

T

W

S

R

P

E

Y

V

S

S

I

A

E

K

T

E

R

R

R

S

K

S

W

L

L

L

E

R

D

K

I

W

R

Y

D

N

A

L

L

M

K

I

A

Q

N

N

K

K

E

D

V

L

G

A

R

R

I

I

L

I

C

T

M

A

E

E

H

S

G

G

K

K

P

P

L

L

Q

K

E

E

A

A

Q

H

G

G

E

E

V

I

D

L

Y

Y

A

S

A

A

G

F

F

F

F

L

D

E

Y

W

C

F

S

S

K

E

H

E

I

A

Q

R

A

R

L

V

D

Y

A

G

H

D

T

I

I

I

P

H

E

T

K

P

P

A

K

K

D

D

C

R

T

R

W

A

T

L

V

V

H

L

Y

K

R

Q

P

P

I

I

G

G

V

V

T

A

G

A

L

V

I
|
I

T
|
T

P
|
P

W
|
W

N
|
N

F

F

P

P

I

S

G

A

M

M

I

I

A

T

K

R

K

K

L

V

S

G

A

A

L

L

A

A

G

G

C

C

P

T

S

V

V

V

I

V

K
|
K

P
|
P

A
|
A

S
x
E

E

D

T

T

P

P

L

F

T

S

M

A

I

L

A

A

L

L

F

A

S

E

L

L

M

A

D

S

K

Q

H

-

T

A

D

G

I

I

P

P

D

S

G

G

M

V

V

Y

N

N

L

V

V

I

-

P

-

C

-

S

M

R

G

K

K

N

A
|
A

S
x
K

V

E

I

V

G

G

K

E

V

A

L

I

C

C

E

T

S

D

P

P

D

L

V

V

P

S

M

K

L

I

S

S

F
|
F

T

T

G

G

S
|
S

T

T

E

T

V
x
T

G

G

R

K

K

I

L

L

I

L

V

H

D

H

T

A

A

A

D

N

Q

S

V

V

K

K

K

R

L

V

A

S

L

M

E
|
E

L

L

G

G

N

L

A

A

P

P

F

F

I

I

V

V

F

F

D

D

D

S

A

A

D

N

L

V

D

D

A

Q

A

A

A

V

D

A

N

G

L

A

I

M

A

A

N

S

K

K

F

F

R

R

G

N

G

T

G

G

Q

Q

T

T

C
x
A

V

V

C

C

A

S

N

N

R

Q

I

F

F

L

V

V

H

Q

E

R

K

G

V

I

A

H

D

D

A

A

F

F

G

V

E

K

K

A

L

F

A

A

E

E

R

A

V

M

N

K

K

K

-

N

M

L

T

R

V

V

G

G

D

N

G

G

I

F

N

E

G

E

D

G

V

T

D

T

L

Q

G

G

P

P

L

L

I

I

N

N

Q

E

A

K

G

A

Y

V

D

E

K

K

V

V

K

E

R

K

H

Q

V

V

Q

N

D

D

A

A

L

V

E

S

K

K

G

G

A

A

T

T

L

V

V

V

A

T

G

G

K

G

K

K

P

R

E

H

D

Q

L

L

G

G

N

K

D

N

L

-

F

F

P

E

P

P

T

T

V

L

H

C

G

N

V

V

N

T

R

Q

D

D

M

M

C

L

C

C

Y

T

Q

H

E

E

E
|
E

T

T

F

F

G

G

P

P

L

L

V

A

P

P

M

V

A

I

L

K

F

F

R

D

T

T

E

E

V

A

I

I

E

A

A

I

G

A

N

N

D

A

T

A

E

D

F

V

G

G

L

L

A

A

S

G

Y

Y

V

F

F

Y

T

S

N

Q

D

D

A

P

E

A

R

Q

A

I

Q

W

R

R

V

V

A

A

A

E

G

Q

L

L

R

E

F

V

G

G

H

M

C

V

G

G

W

V

N

N

T

E

G

G

T

L

G

I

P

S

T

S

P

V

E

E

A

C

P

P

F

F

G

G

M

V

K

K

A

Q

S

S

G

G

I

L

G

G

R

R

E
|
E

G

G

G

S

L

K

E

Y

G

G

L

I

F

D

E

E

F

Y

V

L

E

E

A

L

Q

K

T

Y

V

V

P

C

R

Y

G

G

active site: N155 (= N156), K178 (= K179), E256 (= E255), A290 (≠ C289), E388 (= E387), E465 (= E464)
binding adenosine-5'-diphosphate: I151 (= I152), T152 (= T153), P153 (= P154), W154 (= W155), K178 (= K179), P179 (= P180), A180 (= A181), E181 (≠ S182), A214 (= A213), K215 (≠ S214), F232 (= F231), S235 (= S234), T238 (≠ V237)

2w8qA The crystal structure of human ssadh in complex with ssa. (see paper)
41% identity, 99% coverage: 4:482/483 of query aligns to 5:483/485 of 2w8qA

query
sites
2w8qA

S

S

P

A

L

A

L

L

E

L

K

R

L

T

T

D

G

S

Y

F

I

V

G

G

R

R

W

W

T

L

D

P

N

-

E

-

H

A

G

A

N

A

T

T

F

F

D

P

V

V

Y

Q

N

D

P

P

A

A

T

S

G

G

K

A

V

A

I

L

A

G

Q

M

V

V

A

A

S

D

M

C

S

G

E

V

D

R

E

E

V

A

N

R

A

A

V

V

A

R

S

A

G

A

K

Y

S

E

A

A

L

F

R

C

L

R

T

W

S

R

P

E

Y

V

S

S

I

A

E

K

T

E

R

R

R

S

K

S

W

L

L

L

E

R

D

K

I

W

R

Y

D

N

A

L

L

M

K

I

A

Q

N

N

K

K

E

D

V

L

G

A

R

R

I

I

L

I

C

T

M

A

E

E

H

S

G

G

K

K

P

P

L

L

Q

K

E

E

A

A

Q

H

G

G

E

E

V

I

D

L

Y
|
Y

A

S

A

A

G

F

F

F

F

L

D

E

Y

W

C

F

S

S

K

E

H

E

I

A

Q

R

A

R

L

V

D

Y

A

G

H

D

T

I

I

I

P

H

E

T

K

P

P

A

K

K

D

D

C

R

T

R

W

A

T

L

V

V

H

L

Y

K

R

Q

P

P

I

I

G

G

V

V

T

A

G

A

L

V

I

I

T

T

P

P

W

W

N
|
N

F
|
F

P

P

I

S

G

A

M

M

I

I

A

T

K
x
R

K

K

L

V

S

G

A

A

L

L

A

A

G

G

C

C

P

T

S

V

V

V

I

V

K
|
K

P

P

A

A

S

E

E

D

T

T

P

P

L

F

T

S

M

A

I

L

A

A

L

L

F

A

S

E

L

L

M

A

D

S

K

Q

H

-

T

A

D

G

I

I

P

P

D

S

G

G

M

V

V

Y

N

N

L

V

V

I

-

P

-

C

-

S

M

R

G

K

K

N

A

A

S

K

V

E

I

V

G

G

K

E

V

A

L

I

C

C

E

T

S

D

P

P

D

L

V

V

P

S

M

K

L

I

S

S

F

F

T

T

G

G

S

S

T

T

E

T

V

T

G

G

R

K

K

I

L

L

I

L

V

H

D

H

T

A

A

A

D

N

Q

S

V

V

K

K

K

R

L

V

A

S

L

M

E
|
E

L

L

G

G

N

L

A

A

P

P

F

F

I

I

V

V

F

F

D

D

D

S

A

A

D

N

L

V

D

D

A

Q

A

A

A

V

D

A

N

G

L

A

I

M

A

A

N

S

K

K

F

F

R
|
R

G

N

G

T

G

G

Q

Q

T

T

C
x
A

V
|
V

C

C

A

S

N

N

R

Q

I

F

F

L

V

V

H

Q

E

R

K

G

V

I

A

H

D

D

A

A

F

F

G

V

E

K

K

A

L

F

A

A

E

E

R

A

V

M

N

K

K

K

-

N

M

L

T

R

V

V

G

G

D

N

G

G

I

F

N

E

G

E

D

G

V

T

D

T

L

Q

G

G

P

P

L

L

I

I

N

N

Q

E

A

K

G

A

Y

V

D

E

K

K

V

V

K

E

R

K

H

Q

V

V

Q

N

D

D

A

A

L

V

E

S

K

K

G

G

A

A

T

T

L

V

V

V

A

T

G

G

K

G

K

K

P

R

E

H

D

Q

L

L

G

G

N

K

D

N

L

-

F

F

P

E

P

P

T

T

V

L

H

C

G

N

V

V

N

T

R

Q

D

D

M

M

C

L

C

C

Y

T

Q

H

E

E

E
|
E

T

T

F

F

G

G

P

P

L

L

V

A

P

P

M

V

A

I

L

K

F

F

R

D

T

T

E

E

V

A

I

I

E

A

A

I

G

A

N

N

D

A

T

A

E

D

F

V

G

G

L

L

A

A

S

G

Y

Y

V

F

F

Y

T

S

N

Q

D

D

A

P

E

A

R

Q

A

I

Q

W

R

R

V

V

A

A

A

E

G

Q

L

L

R

E

F

V

G

G

H

M

C

V

G

G

W

V

N

N

T

E

G

G

T

L

G

I

P
x
S

T

S

P

V

E

E

A

C

P

P

F
|
F

G

G

M

V

K

K

A

Q

S

S

G

G

I

L

G

G

R

R

E
|
E

G

G

G

S

L

K

E

Y

G

G

L

I

F

D

E

E

F

Y

V

L

E

E

A

L

Q

K

T

Y

V

V

P

C

R

Y

G

G

active site: N155 (= N156), K178 (= K179), E256 (= E255), A290 (≠ C289), E388 (= E387), E465 (= E464)
binding succinic acid: Y109 (= Y110), F156 (= F157), R163 (≠ K164), E256 (= E255), R284 (= R283), A290 (≠ C289), V291 (= V290), S448 (≠ P447), F454 (= F453)

4neaA 1.90 angstrom resolution crystal structure of betaine aldehyde dehydrogenase (betb) from staphylococcus aureus in complex with NAD+ and bme-free cys289 (see paper)
36% identity, 98% coverage: 6:479/483 of query aligns to 12:491/505 of 4neaA

query
sites
4neaA

L

L

E

K

K

H

L

L

T

S

G

Q

-

R

-

Q

Y

Y

I

I

G

D

G

G

R

E

W

W

T

V

D

E

N

S

E

A

H

N

G

K

N

N

T

T

F

R

D

D

V

I

Y

I

N

N

P

P

A

Y

T

N

G

Q

K

E

V

V

I

I

A

F

Q

T

V

V

A

S

S

E

M

G

S

T

E

K

D

E

E

D

V

A

N

E

A

R

A

A

V

I

A

L

S

A

G

A

K

R

S

R

A

A

L

F

R

E

L

S

-

G

-

E

T

W

S

S

P

Q

Y

E

S

T

I

A

E

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T

R

R

R

G

K

K

W

K

L

V

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A

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I

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A

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D

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K

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L

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G

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K

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L

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D

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F

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Y

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G

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G

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I
|
I

T

T

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|
P

W
|
W

N
|
N

F

Y

P

P

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L

G

L

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A

A

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W

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K

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L

A

A

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G

C

C

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S

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K
|
K

P

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S
x
E

E

I

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T

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P

L

L

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T

M

T

I

I

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F

F

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L

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M

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H

-

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I

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G

G

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N

N

L

L

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M

L

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G

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x
A

A
x
G

S
|
S

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|
G

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D

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|
F

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|
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|
G

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x
G

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x
T

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G

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K
x
H

L
x
I

I

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D

N

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A

A

A

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N

Q

N

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V

K

T

K

N

L

I

A

A

L

L

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|
E

L

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|
G

G

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N

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P

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N

I

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I

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F

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D

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A

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N

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N

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K

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|
C

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C

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L

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D

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K

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G

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A

V

V

A

G

G

G

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K

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D

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D

D

L

L

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D

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M

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|
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|
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N

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G

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L

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Y

A

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V

F

F

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K

D

D

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I

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A

A

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Q

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V

A

A

A

N

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K

L

L

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L

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G

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W

W

I

N

N

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A

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A

A

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P

F

W

G

G

M

Y

K

K

A

Q

S

S

G

G

I

I

G

G

R

R

E
|
E

G

L

G

G

L

K

E

E

G

G

L

L

F

E

E

E

F

Y

V

L

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V

A

S

Q

K

T

H

V

I

active site: N166 (= N156), K189 (= K179), E264 (= E255), C298 (= C289), E399 (= E387), E476 (= E464)
binding nicotinamide-adenine-dinucleotide: I162 (= I152), P164 (= P154), W165 (= W155), N166 (= N156), K189 (= K179), E192 (≠ S182), A221 (≠ K212), G222 (≠ A213), S223 (= S214), G226 (= G217), D227 (≠ K218), F240 (= F231), T241 (= T232), G242 (= G233), G243 (≠ S234), T246 (≠ V237), H249 (≠ K240), I250 (≠ L241), E264 (= E255), G266 (= G257), C298 (= C289), H345 (≠ G336), E399 (= E387), F401 (= F389)

O24174 Betaine aldehyde dehydrogenase 1; OsBADH1; EC 1.2.1.8 from Oryza sativa subsp. japonica (Rice) (see paper)
34% identity, 97% coverage: 13:481/483 of query aligns to 13:489/505 of O24174

query
sites
O24174

Y

F

I

I

G

G

R

G

W

W

T

R

D

E

N

P

E

S

H

L

G

G

N

R

T

R

F

L

D

P

V

V

Y

V

N

N

P

P

A

A

T

T

G

E

K

A

V

T

I

I

A

G

Q

D

V

I

A

P

S

A

M

A

S

T

E

A

D

E

E

D

V

V

N

E

A

L

A

A

V

V

A

S

S

A

G

A

K

R

S

D

A

A

L

F

-

G

-

R

-

D

-

G

R

R

L

H

T

W

S

S

P

R

Y

A

S

P

I

G

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A

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V

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R

R

A

K

K

W

Y

L

L

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K

D

A

I

I

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A

D

A

A

K

L

I

K

K

A

D

N

K

K

K

E

S

E

Y

V

L

G

A

R

L

I

L

L

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C

T

M

L

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D

H

S

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G

K

K

P

P

L

L

Q

D

E

E

A

A

Q

A

G

G

E

D

V

M

D

E

Y

D

A

V

A

A

G

A

F

C

F

F

D

E

Y

Y

C

Y

S

A

K

D

H

L

I

A

Q

E

A

A

L

L

D

D

A

G

H

K

T

Q

-

R

-

A

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P

I

I

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M

K

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N

C

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W

S

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Y

V

V

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L

Y

K

R

E

P

P

I

I

G

G

V

V

T

V

G

G

L

L

I

I

T

T

P

P

W

W

N
|
N

F

Y

P

P

I

L

G

L

M

M

I

A

A

T

K
x
W

K

K

L

V

S

A

A

P

A

A

L

L

A

A

G

G

C

C

P

T

S

A

V

V

I

L

K

K

P

P

A

S

S

E

E

L

T

A

P

S

L

L

T

T

M

C

I

L

A

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L

L

F

G

S

G

L

I

M

C

D

-

K

A

H

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T

I

D

G

I

L

P

P

D

P

G

G

M

V

V

L

N

N

L

I

V

I

M

T

G

G

K

L

A

G

S

T

V

E

I

A

G

G

K

A

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L

L

C

A

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S

S

H

P

P

D

H

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V

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D

M

K

L

I

S

A

F

F

T

T

G

G

S

S

T

T

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E

V

T

G

G

R

K

K

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L

I

I

M

V

I

D

T

T

A

A

S

D

Q

Q

M

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V

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K

K

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L

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L

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L

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G

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L

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V

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F

D

D

A

V

D

D

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D

D

A

K

A

A

A

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N

W

L

A

I

M

A

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N

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K

C

F

F

R

A

G

N

G

A

G

G

Q

Q

T

V

C

C

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S

C

A

A

T

N

S

R

R

I

L

F

L

V

L

H

H

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K

K

V

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A

A

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A

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F

G

L

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L

L

A

V

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A

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W

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A

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K

K

S

M

I

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S

D

D

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G

E

D

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C

D

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L

L

G

G

P

S

L

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I

V

N

S

Q

E

A

G

G

Q

Y

Y

D

Q

K

K

V

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K

M

R

K

H

F

V

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D

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A

A

L

R

E

C

K

E

G

G

A

A

T

T

-

I

L

L

V

Y

A

G

G

G

K

A

K

R

P

P

E

Q

D

H

L

L

G

K

N

R

D

G

L

F

F

F

F

I

P

E

P

P

T

T

V

I

H

T

G

N

V

V

N

S

R

T

D

S

M

M

C

Q

C

I

Y

W

Q

R

E

E

T

V

F

F

G

G

P

P

L

V

V

I

P

C

M

V

A

K

L

E

F

F

R

R

T

T

E

E

E

R

E

E

V

A

I

V

E

E

A

L

G

A

N

N

D

D

T

T

E

H

F

Y

G

G

L

L

A

A

S

G

Y

A

V

V

F

I

T

S

N

N

D

D

A

L

E

E

R

R

A

C

Q

E

R

R

V

I

A

S

A

K

G

A

L

I

R

Q

F

S

G

G

H

I

C

V

G

W

W

I

N

N

T

C

G

S

T

Q

G

P

P

C

T

F

P

V

E

Q

A

A

P

P

F

W

G

G

M

N

K

K

A

R

S

S

G

G

I

F

G

G

R

R

E

E

G

L

G

G

L

Q

E

W

G

G

L

L

F

D

E

N

F

Y

V

L

E

S

A

V

Q

K

T

Q

V

V

P

T

R

K

N164 (= N156) mutation to A: Slightly reduced affinity for NAD, 6-fold enhanced affinity for both gamma-4-aminobutyraldehyde (GAB-ald) and betaine aldehyde (Bet-ald), but 2-fold decrease in catalytic efficiency.
W172 (≠ K164) mutation to A: Slightly reduced affinity for NAD, enhanced affinity for both betaine aldehyde (Bet-ald) (10-fold) and gamma-4-aminobutyraldehyde (GAB-ald) (2-fold).; mutation to F: Slightly reduced affinity for NAD, but 6-fold enhanced affinity for both gamma-4-aminobutyraldehyde (GAB-ald) and betaine aldehyde (Bet-ald) and 2-fold increase in catalytic efficiency towards GAB-ald.

6popA Crystal structure of daua in complex with NADP+ (see paper)
34% identity, 98% coverage: 13:483/483 of query aligns to 7:473/475 of 6popA

query
sites
6popA

Y

Y

I

I

G

G

R

K

W

L

T

I

D

T

N

S

E

D

H

A

G

-

N

-

T

T

F

T

D

E

V

I

Y

I

N

N

P

P

A

A

T

T

G

L

K

E

V

I

I

V

A

G

Q

E

V

I

A

S

S

A

M

A

S

G

E

I

D

N

E

E

V

A

N

N

A

M

A

A

V

L

A

E

S

S

G

A

K

Q

S

E

A

A

L

F

R

S

L

S

T

W

S

S

P

T

Y

T

S

P

I

A

E

I

T

E

R

R

R

A

K

Q

W

W

L

M

E

L

D

K

I

L

R

R

D

D

A

A

L

V

K

I

A

A

N

N

K

E

E

Q

E

H

V

L

G

R

R

E

I

C

L

V

C

H

M

L

E

E

H

M

G

A

K

K

P

P

L

W

Q

Q

E

S

A

T

Q

A

G

D

E

D

V

F

D

Q

Y

M

A

L

A

V

G

D

F

S

F

L

D

N

Y

F

C

Y

S

A

K

D

H

A

I

I

Q

V

A

N

L

I

D

A

A

D

H

E

T

E

I

I

P

K

E

D

K

N

P

E

K

G

D

T

C

H

T

S

W

H

T

V

V

L

H

S

Y

R

R

E

P

P

I

V

G

G

V

V

T

A

G

A

L

A

I
x
F

T
x
L

P
x
A

W
|
W

N
|
N

F

F

P

P

I

L

G

L

M

N

I

L

A

A

K

Y

K

K

L

L

S

G

A

P

A

A

L

M

A

A

G

G

C

C

P

P

S

L

V

V

I

V

K
|
K

P

P

A
x
S

S
|
S

E

K

T

T

P

P

L

L

T

S

M

A

I

Y

A

A

L

V

F

G

S

E

L

L

M

C

D

E

K

Q

H

-

T

I

D

G

I

L

P

P

D

A

G

G

M

V

V

V

N

N

L

I

V

L

M

S

G

G

K

M

A
x
D

S

S

V

T

I

V

G
|
G

K
x
D

V

A

L

I

C

S

E

A

S

S

P

T

D

I

V

P

P

S

M

V

L

L

S

T

F
x
L

T
x
I

G
|
G

S
|
S

T

T

E

N

V
|
V

G

G

R

K

K
x
H

L

V

I

I

V

A

D

T

T

G

A

A

D

T

Q

S

V

I

K

K

K

R

L

Y

A

S

L

M

E
|
E

L
|
L

G
|
G

G

G

N

N

A

A

P

P

F

A

I

I

V

V

F

C

D

S

D

D

A

A

D

N

L

L

D

D

A

N

A

A

D

D

N

V

L

I

I

C

A

G

N

V

K

K

F

F

R

A

G

N

G

A

G

G

Q

Q

T

I

C
|
C

V

V

C

T

A

P

N

N

R

R

I

V

F

F

V

V

H

H

E

E

K

S

V

V

A

A

D

D

A

E

F

F

G

I

E

E

K

K

L

V

A

L

E

T

R

R

V

A

N

K

K

A

M

V

T

K

V

V

G

G

D

F

G

D

I

K

N

N

G

E

D

A

V

I

D

D

L

M

G

G

P

P

L

V

I

M

N

D

Q

A

A

N

G

S

Y

W

D

Q

K

R

V

I

K

D

R

E

H

L

V

V

Q

K

D

D

A

A

L

Q

E

Q

K

N

G

G

A

A

T

Q

L

L

-

Q

V

L

A

G

G

G

K

K

P

P

E

T

D

G

L

V

G

-

N

N

D

G

L

Y

F

F

F

Y

P

E

P

P

T

T

V

V

V

L

H

T

G

N

V

V

N

D

R

S

D

S

M

M

C

K

C

I

Y

Y

Q

K

E

D

E
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E

T

I

F
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F

G

G

P

P

L

V

V

I

P

S

M

I

A

I

L

I

F

F

R

S

T

D

E

N

E

E

E

Q

V

V

I

L

E

S

A

D

G

A

N

N

D

D

T

T

E

D

F

A

G

G

L
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L

A

S

S

S

Y

F

V

I

F

F

T

S

N

S

D

N

A

E

E

D

R

T

A

I

Q

S

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Y

V

F

A

A

A

K

G

H

L

L

R

R

F

F

G

G

H

E

C

V

G

Q

W

V

N

N

T

-

G

G

T

I

G

K

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Y

T

S

P

I

E

N

A

L

P

P

F
x
H

G

F

G

G

M

I

K

K

A

Q

S

S

G

G

I

V

G

G

R

V

E
x
D

G

C

G

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L

L

E

L

G

A

L

L

F

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D

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Y

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A

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V

V

P

S

R

R

G

A

F

L

active site: N148 (= N156), E246 (= E255), C280 (= C289), D454 (≠ E464)
binding nadp nicotinamide-adenine-dinucleotide phosphate: F144 (≠ I152), L145 (≠ T153), A146 (≠ P154), W147 (= W155), N148 (= N156), K171 (= K179), S173 (≠ A181), S174 (= S182), D204 (≠ A213), G208 (= G217), D209 (≠ K218), L222 (≠ F231), I223 (≠ T232), G224 (= G233), S225 (= S234), V228 (= V237), H231 (≠ K240), E246 (= E255), L247 (= L256), G248 (= G257), C280 (= C289), E378 (= E387), F380 (= F389), L406 (= L415), H443 (≠ F453)

4pz2B Structure of zm aldh2-6 (rf2f) in complex with NAD (see paper)
36% identity, 99% coverage: 1:479/483 of query aligns to 2:484/494 of 4pz2B

query
sites
4pz2B

M

L

I

F

E

D

S

V

P

P

L

E

L

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A

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G

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I

I

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V

A

A

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E

M

A

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E

K

D

E

E

D

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N

D

A

L

A

A

V

V

A

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S

A

G

A

K

R

S

A

A

A

L

F

-

D

-

H

-

G

-

E

-

W

-

P

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M

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G

P

-

Y

-

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-

I

-

E

S

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I

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M

E

A

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D

A

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A

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A

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L

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A

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G

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K

-

H

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P

L

A

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T

A

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Q

A

G

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E

D

V

V

D

G

Y

N

A

A

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Y

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-

E

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A

G

G

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I
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I

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x
I

P
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P

W
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W

N
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N

F

F

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P

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M
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M

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F

A

A

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A

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A

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x
E

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L

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A

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L

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-

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Q

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N

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A
x
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x
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x
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x
L

L
x
I

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A

A

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Q

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-

N

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E

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G

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F

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A

A

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A

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A

A

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G

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K

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Y

F

Y

F

I

P

E

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P

T

T

V

V

V

F

H

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V

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M

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E
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F

G

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P

L

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V

M

P

C

M

L

A

M

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F

F

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K

T

T

E

V

E

E

V

A

I

I

E

A

A

R

G

A

N

N

D

D

T

T

E

R

F

Y

G

G

L
|
L

A

G

S

A

Y

G

V

V

F

V

T

T

N

R

D

D

A

L

E

D

R

V

A

A

Q

N

R

R

V

V

A

V

A

R

G

S

L

V

R

R

F

A

G

G

H

V

C

V

G

W

W

V

N

N

T

C

G

Y

T

F

G

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M

T

G

P

S

E

D

A

C

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P

F
|
F

G

G

M

R

K

K

A

M

S

S

G

G

I

F

G

G

R

K

E
x
D

G

E

G

G

L

M

E

H

G

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A

A

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K

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V

V

active site: N159 (= N156), K182 (= K179), E258 (= E255), C292 (= C289), E392 (= E387), D469 (≠ E464)
binding nicotinamide-adenine-dinucleotide: I155 (= I152), I156 (≠ T153), P157 (= P154), W158 (= W155), N159 (= N156), M164 (= M161), K182 (= K179), P183 (= P180), A184 (= A181), E185 (≠ S182), G215 (≠ A213), P216 (≠ S214), G219 (= G217), A220 (≠ K218), F233 (= F231), T234 (= T232), G235 (= G233), S236 (= S234), V239 (= V237), L242 (≠ K240), I243 (≠ L241), E258 (= E255), L259 (= L256), G260 (= G257), C292 (= C289), E392 (= E387), F394 (= F389), L420 (= L415), F458 (= F453)

4o6rA Crystal structure of a putative aldehyde dehydrogenase from burkholderia cenocepacia
35% identity, 97% coverage: 13:479/483 of query aligns to 7:475/489 of 4o6rA

query
sites
4o6rA

Y

F

I

I

G

D

G

G

R

R

W

F

T

V

D

D

N

A

E

V

H

D

G

R

N

G

T

T

F

I

D

D

V

V

Y

L

N

N

P

P

A

H

T

D

G

G

K

S

V

V

I

I

A

T

Q

K

V

I

A

A

S

A

M

A

S

T

E

A

D

A

E

D

V

V

N

D

A

L

A

A

V

V

A

D

S

A

G

A

K

T

S

R

A

A

L

F

R

P

L

A

T

W

S

S

P

A

Y

M

S

P

I

A

E

A

T

E

R

R

R

G

K

R

W

L

L

L

E

L

D

R

I

L

R

A

D

D