SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF349 FitnessBrowser__Marino:GFF349 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

5i92F Crystal structure of glutamate-1-semialdehyde 2,1- aminomutase (gsa) from pseudomonas aeruginosa
68% identity, 100% coverage: 1:424/426 of query aligns to 1:419/420 of 5i92F

query
sites
5i92F

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A

active site: V19 (= V19), Y142 (= Y142), E201 (= E204), D234 (= D237), M237 (= M240), K262 (= K265), A395 (= A400)
binding leucine: I339 (= I344), R373 (= R378), R376 (≠ K381), F377 (= F382), L380 (≠ G385), A417 (≠ V422), F418 (≠ M423), A419 (= A424)

Sites not aligning to the query:

binding leucine: 420

P23893 Glutamate-1-semialdehyde 2,1-aminomutase; GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT; EC 5.4.3.8 from Escherichia coli (strain K12) (see paper)
64% identity, 100% coverage: 1:426/426 of query aligns to 1:426/426 of P23893

query
sites
P23893

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K265 (= K265) mutation to R: 2% of wild-type activity.

3bs8A Crystal structure of glutamate 1-semialdehyde aminotransferase complexed with pyridoxamine-5'-phosphate from bacillus subtilis (see paper)
56% identity, 99% coverage: 4:426/426 of query aligns to 7:427/430 of 3bs8A

query
sites
3bs8A

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A

K

E

K

K

V

V

M

F

A

A

T

E

L

I

active site: V22 (= V19), Y145 (= Y142), E207 (= E204), D240 (= D237), M243 (= M240), K268 (= K265), G401 (≠ A400)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: S117 (= S114), G118 (= G115), T119 (= T116), Y145 (= Y142), H146 (= H143), G147 (= G144), E207 (= E204), N212 (= N209), D240 (= D237), V242 (= V239), M243 (= M240), K268 (= K265)

6w80A Crystal structure of glutamate-1-semialdehyde 2,1-aminomutase from stenotrophomonas maltophilia k279a in complex with plp
58% identity, 98% coverage: 1:416/426 of query aligns to 6:419/430 of 6w80A

query
sites
6w80A

M

M

T

N

H

H

-

D

-

Q

S

S

E

H

T

A

L

L

F

F

E

S

Q

R

A

A

Q

Q

K

Q

Y

L

I

L

P

P

G

G

G

G

V
|
V

N

N

S

S

P

P

V

V

R

R

A

A

F

F

R

K

G

S

V

V

G

G

G

G

T

E

P

P

I

F

F

F

F

V

K

E

H

R

A

A

Q

D

G

G

A

A

Y

Y

L

L

Y

Y

D

D

E

V

D

D

D

G

Q

N

R

R

Y

Y

I

I

D

D

Y

Y

I

V

G

G

S

S

W

W

G

G

P

P

M

M

I

I

L

V

G

G

H

H

G

N

D

H

Q

P

R

A

I

V

K

R

D

Q

A

A

L

V

H

K

A

K

Q

A

V

I

D

D

L

N

G

G

V

L

G

S

Y

F

G

G

A

A

P

P

T

C

A

A

L

G

E

E

T

V

E

T

M

M

A

A

K

E

K

T

V

I

C

T

E

R

L

L

M

V

P

P

S

S

I

C

E

E

L

M

V

V

R

R

M

M

V

V

N

N

S
|
S

G
|
G

T
|
T

E

E

A

A

T

T

M

L

S

S

T

A

V

I

R

R

L

L

A

A

R

R

G

G

Y

A

T

T

G

G

R

R

D

N

K

R

I

I

V

V

K

K

F

F

E

E

G

G

C

C

Y
|
Y

H
|
H

G
|
G

H

H

V

G

D

D

S

S

L

F

L

L

V

V

K

K

A

A

G

-

S

-

G

-

A

M

L

L

T

T

L

L

G

G

V

V

P

P

N

T

S

S

P

P

G

G

I

V

P

P

A

A

S

G

L

L

A

S

E

E

H

L

T

T

I

L

T

T

L

L

T

P

Y

Y

N

N

D

D

I

F

D

E

S

A

V

A

R

T

E

A

C

L

F

F

R

E

E

Q

M

Q

G

G

D

D

Q

D

I

I

A

A

A

G

I

L

I

I

V

I

E
|
E

P

P

V

V

A

V

G

G

N
|
N

M

A

N

N

C

C

I

I

P

P

P

P

V

R

P

E

G

G

F

Y

L

L

E

Q

G

H

L

L

R

R

E

A

V

L

C

C

D

T

E

K

H

H

G

G

T

A

V

L

L

L

I

I

F

F

D
|
D

E

E

V
|
V

M
|
M

T

T

G

G

F

F

R

R

V

V

S

A

L

L

G

G

G

G

A

A

Q

Q

G

A

L

H

Y

Y

G

G

V

I

T

T

P

P

D

D

L

L

T

T

A

T

L

F

G

G

K
|
K

V

I

I

I

G

G

G

G

G

G

L

M

P

P

V

V

G

G

A

A

F

Y

G

G

G

G

K

R

R

R

E

E

I

L

M

M

E

Q

H

Q

I

I

S

A

P

P

L

A

G

G

P

P

V

I

Y

Y

Q

Q

A

A

G

G

T

T

L

L

S

S

G

G

N

N

P

P

L

V

A

A

M

M

C

A

A

A

G

G

L

L

T

A

T

M

L

L

N

E

A

L

I

V

S

Q

E

Q

P

P

G

G

F

F

H

H

D

A

R

D

L

L

T

A

E

E

K

R

T

T

N

A

A

R

V

L

R

C

D

A

G

G

L

L

K

E

E

A

A

A

A

A

D

A

A

D

T

A

G

G

I

V

P

A

L

V

T

T

V

T

Q

T

S

R

A

V

G

G

A

A

M

M

F

F

G

G

F

L

F

F

F

F

T

T

E

S

E

E

S

-

S

K

V

V

T

E

R

T

F

Y

D

A

Q

Q

V

A

M

T

G

A

C

C

D

D

V

I

E

P

R

A

F

F

K

N

K

R

F

F

F

F

Q

H

G

A

M

M

L

L

K

E

E

Q

G

G

V

V

Y

F

L

L

A

A

P

P

S

S

A

A

F

Y

E

E

A

A

G

G

F

F

T

L

T

S

A

S

A

A

L

H

S

D

E

D

Q

A

D

V

I

I

A

E

D

A

T

T

V

L

active site: V26 (= V19), Y149 (= Y142), D241 (= D237), K269 (= K265)
binding pyridoxal-5'-phosphate: S121 (= S114), G122 (= G115), T123 (= T116), Y149 (= Y142), H150 (= H143), G151 (= G144), E208 (= E204), N213 (= N209), D241 (= D237), V243 (= V239), M244 (= M240), K269 (= K265)

2gsaA Crystal structure of glutamate-1-semialdehyde aminomutase (aminotransferase, wild-type form) (see paper)
55% identity, 99% coverage: 4:426/426 of query aligns to 6:427/427 of 2gsaA

query
sites
2gsaA

S

S

E

D

T

E

L

I

F

F

E

A

Q

A

A

A

Q

Q

K

K

Y

L

I

M

P

P

G

G

G

G

V
|
V

N

S

S

S

P

P

V

V

R

R

A

A

F

F

R

K

G

S

V

V

G

G

G

G

T

Q

P

P

I

I

F

V

F

F

K

D

H

R

A

V

Q

K

G

D

A

A

Y

Y

L

A

Y

W

D

D

E

V

D

D

D

G

Q

N

R

R

Y

Y

I

I

D

D

Y

Y

I

V

G

G

S

T

W

W

G

G

P

P

M

A

I

I

L

C

G

G

H

H

G

A

D

H

Q

P

R

E

I

V

K

I

D

E

A

A

L

L

H

K

A

V

Q

A

V

M

D

E

L

K

G

G

V

T

G

S

Y

F

G

G

A

A

P

P

T

C

A

A

L

L

E

E

T

N

E

V

M

L

A

A

K

E

K

M

V

V

C

N

E

D

L

A

M

V

P

P

S

S

I

I

E

E

L

M

V

V

R

R

M

F

V

V

N

N

S
|
S

G
|
G

T
|
T

E

E

A

A

T

C

M

M

S

A

T

V

V

L

R

R

L

L

A

M

R

R

G

A

Y

Y

T

T

G

G

R

R

D

D

K

K

I

I

V

I

K

K

F

F

E

E

G

G

C

C

Y
|
Y

H
|
H

G
|
G

H

H

V

A

D

D

S

M

L

F

L

L

V

V

K

K

A

A

G

G

S

S

G

G

A

V

L

A

T

T

L

L

G

G

V

L

P

P

N

S

S

S

P

P

G

G

I

V

P

P

A

K

S

K

L

T

A

T

E

A

H

N

T

T

I

L

T

T

L

T

T

P

Y

Y

N

N

D

D

I

L

D

E

S

A

V

V

R

K

E

A

C

L

F

F

R

A

E

E

M

N

G

P

D

G

Q

E

I

I

A

A

A

G

I

V

I

I

V

L

E
|
E

P

P

V

I

A

V

G

G

N
|
N

M

S

N

G

C

F

I

I

P

V

P

P

V

D

P

A

G

G

F

F

L

L

E

E

G

G

L

L

R

R

E

E

V

I

C

T

D

L

E

E

H

H

G

D

T

A

V

L

L

L

I

V

F

F

D
|
D

E

E

V
|
V

M
|
M

T

T

G

G

F

F

R

R

V

I

S

A

L

Y

G

G

G

G

A

V

Q

Q

G

E

L

K

Y

F

G

G

V

V

T

T

P

P

D

D

L

L

T

T

A

T

L

L

G

G

K
|
K

V

I

I

I

G

G

G

G

G

G

L

L

P

P

V

V

G

G

A

A

F

Y

G

G

G

G

K

K

R

R

E

E

I

I

M

M

E

Q

H

L

I

V

S

A

P

P

L

A

G

G

P

P

V

M

Y

Y

Q

Q

A

A

G
|
G

T
|
T

L

L

S

S

G

G

N

N

P

P

L

L

A

A

M

M

C

T

A

A

G

G

L

I

T

K

T

T

L

L

N

E

A

L

I

L

S

R

E

Q

P

P

G

G

F

T

H

Y

D

E

R

Y

L

L

T

D

E

Q

K

I

T

T

N

K

A

R

V

L

R

S

D

D

G

G

L

L

K

L

E

A

A

I

A

A

D

Q

A

E

T

T

G

G

I

H

P

A

L

A

T

C

V

G

Q

G

S

Q

A

V

G

S

A

G

M

M

F

F

G

G

F

F

F

F

F

F

T

T

E

-

E

E

S

G

S

P

V

V

T

H

R

N

F

Y

D

E

Q

D

V

A

M

K

G

K

C

S

D

D

V

L

E

Q

R

K

F

F

K

S

K

R

F

F

F

H

Q

R

G

G

M

M

L

L

K

E

E

Q

G

G

V

I

Y

Y

L

L

A

A

P

P

S

S

A

Q

F

F

E

E

A
|
A

G

G

F

F

T

T

T

S

A

L

A

A

L

H

S

T

E

E

Q

E

D

D

I

I

A

D

D

A

T

T

V

L

A

A

A

A

A

A

K

R

K

T

V

V

M

M

A

S

T

A

L

L

active site: V21 (= V19), Y144 (= Y142), E206 (= E204), D239 (= D237), M242 (= M240), K267 (= K265), A401 (= A400)
binding pyridoxal-5'-phosphate: G298 (= G296), T299 (= T297)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: S116 (= S114), G117 (= G115), T118 (= T116), Y144 (= Y142), H145 (= H143), G146 (= G144), E206 (= E204), N211 (= N209), D239 (= D237), V241 (= V239), M242 (= M240), K267 (= K265)

3usfA Crystal structure of dava-4
54% identity, 99% coverage: 4:426/426 of query aligns to 6:427/427 of 3usfA

query
sites
3usfA

S

S

E

D

T

E

L

I

F

F

E

A

Q

A

A

A

Q

Q

K

K

Y

L

I

M

P

P

G

G

G

G

V
|
V

N

S

S
|
S

P

P

V
|
V

R

R

A

A

F

F

R

K

G

S

V

V

G

G

G

G

T

Q

P

P

I

I

F

V

F

F

K

D

H

R

A

V

Q

K

G

D

A

A

Y

Y

L

A

Y

W

D

D

E

V

D

D

D

G

Q

N

R

R

Y

Y

I

I

D

D

Y

Y

I

V

G

G

S

T

W
|
W

G

G

P

P

M

A

I

I

L

C

G

G

H

H

G

A

D

H

Q

P

R

E

I

V

K

I

D

E

A

A

L

L

H

K

A

V

Q

A

V

M

D

E

L

K

G

G

V

T

G

S

Y

F

G

G

A

A

P

P

T

C

A

A

L

L

E

E

T

N

E

V

M

L

A

A

K

E

K

M

V

V

C

N

E

D

L

A

M

V

P

P

S

S

I

I

E

E

L

M

V

V

R

R

M

F

V

V

N

N

S
|
S

G
|
G

T
|
T

E

E

A

A

T

C

M

M

S

A

T

V

V

L

R

R

L

I

A

M

R

R

G

A

Y

Y

T

T

G

G

R

R

D

D

K

K

I

I

V

I

K

K

F

F

E

E

G

G

C

C

Y
|
Y

H
|
H

G
|
G

H

H

V

A

D

D

S

M

L

F

L

L

V

V

K

K

A

A

G

G

S
|
S

G

G

A

V

L

A

T

T

L

L

G

G

V

L

P

P

N

S

S

S

P

P

G

G

I

V

P

P

A

K

S

K

L

T

A

T

E

A

H

N

T

T

I

L

T

T

L

T

T

P

Y

Y

N

N

D

D

I

L

D

E

S

A

V

V

R

K

E

A

C

L

F

F

R

A

E

E

M

N

G

P

D

G

Q

E

I

I

A

A

A

G

I

V

I

I

V

L

E
|
E

P

P

V

I

A

V

G

G

N
|
N

M

S

N

G

C

F

I

I

P

V

P

P

V

D

P

A

G

G

F

F

L

L

E

E

G

G

L

L

R

R

E

E

V

I

C

T

D

L

E

E

H

H

G

D

T

A

V

L

L

L

I

V

F

F

D
|
D

E

E

V
|
V

M
|
M

T

T

G

G

F

F

R

R

V

I

S

A

L

Y

G

G

G

G

A

V

Q

Q

G

E

L

K

Y

F

G

G

V

V

T

T

P

P

D

D

L

L

T

T

A

T

L

L

G

G

K
|
K

V

I

I

I

G

G

G

G

G

G

L

L

P

P

V

V

G

G

A

A

F

Y

G

G

G

G

K

K

R

R

E

E

I

I

M

M

E

Q

H

L

I

V

S

A

P

P

L

A

G

G

P

P

V

M

Y

Y

Q

Q

A

A

G
|
G

T
|
T

L

L

S

S

G

G

N

N

P

P

L

L

A

A

M

M

C

T

A

A

G

G

L

I

T

K

T

T

L

L

N

E

A

L

I

L

S

R

E

Q

P

P

G

G

F

T

H

Y

D

E

R

Y

L

L

T

D

E

Q

K

I

T

T

N

K

A

R

V

L

R

S

D

D

G

G

L

L

K

L

E

A

A

I

A

A

D

Q

A

E

T

T

G

G

I

H

P

A

L

A

T

C

V

G

Q

G

S

Q

A

V

G

S

A

G

M

M

F

F

G

G

F

F

F

F

F

F

T

T

E

-

E

E

S

G

S

P

V

V

T

H

R

N

F

Y

D

E

Q

D

V

A

M

K

G

K

C

S

D

D

V

L

E

Q

R

K

F

F

K

S

K

R

F

F

F

H

Q

R

G

G

M

M

L

L

K

E

E

Q

G

G

V

I

Y

Y

L

L

A

A

P

P

S

S

A

Q

F

F

E
|
E

A
|
A

G

G

F

F

T

T

T

S

A

L

A

A

L

H

S

T

E

E

Q

E

D

D

I

I

A

D

D

A

T

T

V

L

A

A

A

A

A

A

K

R

K

T

V

V

M

M

A

S

T

A

L

L

active site: V21 (= V19), Y144 (= Y142), D239 (= D237), M242 (= M240), K267 (= K265), A401 (= A400)
binding (4s)-4,5-diaminopentanoic acid: S23 (= S21), V25 (= V23), W61 (= W59), S157 (= S155), N211 (= N209), M242 (= M240), K267 (= K265), E400 (= E399)
binding (5-hydroxy-4,6-dimethylpyridin-3-yl)methyl dihydrogen phosphate: S116 (= S114), G117 (= G115), T118 (= T116), Y144 (= Y142), H145 (= H143), G146 (= G144), E206 (= E204), N211 (= N209), D239 (= D237), V241 (= V239), M242 (= M240), K267 (= K265), G298 (= G296), T299 (= T297)

3fq7A Gabaculine complex of gsam (see paper)
54% identity, 99% coverage: 4:426/426 of query aligns to 6:427/427 of 3fq7A

query
sites
3fq7A

S

S

E

D

T

E

L

I

F

F

E

A

Q

A

A

A

Q

Q

K

K

Y

L

I

M

P

P

G

G

G

G

V
|
V

N

S

S
|
S

P

P

V
|
V

R

R

A

A

F

F

R

K

G

S

V

V

G

G

G

G

T

Q

P

P

I

I

F

V

F

F

K

D

H

R

A

V

Q

K

G

D

A

A

Y

Y

L

A

Y

W

D

D

E

V

D

D

D

G

Q

N

R

R

Y

Y

I

I

D

D

Y

Y

I

V

G

G

S

T

W
|
W

G

G

P

P

M

A

I

I

L

C

G

G

H

H

G

A

D

H

Q

P

R

E

I

V

K

I

D

E

A

A

L

L

H

K

A

V

Q

A

V

M

D

E

L

K

G

G

V

T

G

S

Y

F

G

G

A

A

P

P

T

C

A

A

L

L

E

E

T

N

E

V

M

L

A

A

K

E

K

M

V

V

C

N

E

D

L

A

M

V

P

P

S

S

I

I

E

E

L

M

V

V

R

R

M

F

V

V

N

N

S
|
S

G
|
G

T
|
T

E

E

A

A

T
x
C

M

M

S

A

T

V

V

L

R

R

L

I

A

M

R

R

G

A

Y

Y

T

T

G

G

R

R

D

D

K

K

I

I

V

I

K

K

F

F

E

E

G

G

C

C

Y
|
Y

H
|
H

G
|
G

H

H

V

A

D

D

S

M

L

F

L

L

V

V

K

K

A

A

G

G

S
|
S

G

G

A

V

L

A

T

T

L

L

G

G

V

L

P

P

N

S

S

S

P

P

G

G

I

V

P

P

A

K

S

K

L

T

A

T

E

A

H

N

T

T

I

L

T

T

L

T

T

P

Y

Y

N

N

D

D

I

L

D

E

S

A

V

V

R

K

E

A

C

L

F

F

R

A

E

E

M

N

G

P

D

G

Q

E

I

I

A

A

A

G

I

V

I

I

V

L

E
|
E

P

P

V

I

A

V

G

G

N
|
N

M

S

N

G

C

F

I

I

P

V

P

P

V

D

P

A

G

G

F

F

L

L

E

E

G

G

L

L

R

R

E

E

V

I

C

T

D

L

E

E

H

H

G

D

T

A

V

L

L

L

I

V

F

F

D
|
D

E

E

V
|
V

M
|
M

T

T

G

G

F

F

R

R

V

I

S

A

L

Y

G

G

G

G

A

V

Q

Q

G

E

L

K

Y

F

G

G

V

V

T

T

P

P

D

D

L

L

T

T

A

T

L

L

G

G

K
|
K

V

I

I

I

G

G

G

G

G

G

L

L

P

P

V

V

G

G

A

A

F

Y

G

G

G

G

K

K

R

R

E

E

I

I

M

M

E

Q

H

L

I

V

S

A

P

P

L

A

G

G

P

P

V

M

Y

Y

Q

Q

A
|
A

G
|
G

T
|
T

L

L

S

S

G

G

N

N

P

P

L

L

A

A

M

M

C

T

A

A

G

G

L

I

T

K

T

T

L

L

N

E

A

L

I

L

S

R

E

Q

P

P

G

G

F

T

H

Y

D

E

R

Y

L

L

T

D

E

Q

K

I

T

T

N

K

A

R

V

L

R

S

D

D

G

G

L

L

K

L

E

A

A

I

A

A

D

Q

A

E

T

T

G

G

I

H

P

A

L

A

T

C

V

G

Q

G

S

Q

A

V

G

S

A

G

M

M

F

F

G

G

F

F

F

F

F

F

T

T

E

-

E

E

S

G

S

P

V

V

T

H

R

N

F

Y

D

E

Q

D

V

A

M

K

G

K

C

S

D

D

V

L

E

Q

R

K

F

F

K

S

K

R

F

F

F

H

Q

R

G

G

M

M

L

L

K

E

E

Q

G

G

V

I

Y

Y

L

L

A

A

P

P

S

S

A

Q

F

F

E
|
E

A
|
A

G

G

F

F

T

T

T

S

A

L

A

A

L

H

S

T

E

E

Q

E

D

D

I

I

A

D

D

A

T

T

V

L

A

A

A

A

A

A

K

R

K

T

V

V

M

M

A

S

T

A

L

L

active site: V21 (= V19), Y144 (= Y142), D239 (= D237), M242 (= M240), K267 (= K265), A401 (= A400)
binding 3-[o-phosphonopyridoxyl]--amino-benzoic acid: S23 (= S21), V25 (= V23), W61 (= W59), S116 (= S114), G117 (= G115), T118 (= T116), C121 (≠ T119), Y144 (= Y142), H145 (= H143), G146 (= G144), S157 (= S155), E206 (= E204), N211 (= N209), D239 (= D237), V241 (= V239), M242 (= M240), K267 (= K265), A297 (= A295), G298 (= G296), T299 (= T297), E400 (= E399)

2hp2A Inter-subunit signaling in gsam (see paper)
54% identity, 99% coverage: 4:426/426 of query aligns to 6:427/427 of 2hp2A

query
sites
2hp2A

S

S

E

D

T

E

L

I

F

F

E

A

Q

A

A

A

Q

Q

K

K

Y

L

I

M

P

P

G

G

G

G

V
|
V

N

S

S
|
S

P

P

V
|
V

R

R

A

A

F

F

R

K

G

S

V

V

G

G

G

G

T

Q

P

P

I

I

F

V

F

F

K

D

H

R

A

V

Q

K

G

D

A

A

Y

Y

L

A

Y

W

D

D

E

V

D

D

D

G

Q

N

R

R

Y

Y

I

I

D

D

Y

Y

I

V

G

G

S

T

W
|
W

G

G

P

P

M

A

I

I

L

C

G

G

H

H

G

A

D

H

Q

P

R

E

I

V

K

I

D

E

A

A

L

L

H

K

A

V

Q

A

V

M

D

E

L

K

G

G

V

T

G

S

Y

F

G

G

A

A

P

P

T

C

A

A

L

L

E

E

T

N

E

V

M

L

A

A

K

E

K

M

V

V

C

N

E

D

L

A

M

V

P

P

S

S

I

I

E

E

L

M

V

V

R

R

M

F

V

V

N

N

S
|
S

G
|
G

T
|
T

E

E

A

A

T
x
C

M

M

S

A

T

V

V

L

R

R

L

I

A

M

R

R

G

A

Y

Y

T

T

G

G

R

R

D

D

K

K

I

I

V

I

K

K

F

F

E

E

G

G

C

C

Y
|
Y

H
|
H

G
|
G

H

H

V

A

D

D

S

M

L

F

L

L

V

V

K

K

A

A

G

G

S

S

G

G

A

V

L

A

T

T

L

L

G

G

V

L

P

P

N

S

S

S

P

P

G

G

I

V

P

P

A

K

S

K

L

T

A

T

E

A

H

N

T

T

I

L

T

T

L

T

T

P

Y

Y

N

N

D

D

I

L

D

E

S

A

V

V

R

K

E

A

C

L

F

F

R

A

E

E

M

N

G

P

D

G

Q

E

I

I

A

A

A

G

I

V

I

I

V

L

E
|
E

P

P

V

I

A

V

G

G

N
|
N

M

S

N

G

C

F

I

I

P

V

P

P

V

D

P

A

G

G

F

F

L

L

E

E

G

G

L

L

R

R

E

E

V

I

C

T

D

L

E

E

H

H

G

D

T

A

V

L

L

L

I

V

F

F

D
|
D

E

E

V
|
V

M
|
M

T

T

G

G

F

F

R

R

V

I

S

A

L

Y

G

G

G

G

A

V

Q

Q

G

E

L

K

Y

F

G

G

V

V

T

T

P

P

D

D

L

L

T

T

A

T

L

L

G

G

K
|
K

V

I

I

I

G

G

G

G

G

G

L

L

P

P

V

V

G

G

A

A

F

Y

G

G

G

G

K

K

R

R

E

E

I

I

M

M

E

Q

H

L

I

V

S

A

P

P

L

A

G

G

P

P

V

M

Y

Y

Q

Q

A

A

G
|
G

T
|
T

L

L

S

S

G

G

N

N

P

P

L

L

A

A

M

M

C

T

A

A

G

G

L

I

T

K

T

T

L

L

N

E

A

L

I

L

S

R

E

Q

P

P

G

G

F

T

H

Y

D

E

R

Y

L

L

T

D

E

Q

K

I

T

T

N

K

A

R

V

L

R

S

D

D

G

G

L

L

K

L

E

A

A

I

A

A

D

Q

A

E

T

T

G

G

I

H

P

A

L

A

T

C

V

G

Q

G

S

Q

A

V

G

S

A

G

M

M

F

F

G

G

F

F

F

F

F

F

T

T

E

-

E

E

S

G

S

P

V

V

T

H

R

N

F

Y

D

E

Q

D

V

A

M

K

G

K

C

S

D

D

V

L

E

Q

R

K

F

F

K

S

K

R

F

F

F

H

Q

R

G

G

M

M

L

L

K

E

E

Q

G

G

V

I

Y

Y

L

L

A

A

P

P

S

S

A

Q

F

F

E
|
E

A
|
A

G

G

F

F

T

T

T

S

A

L

A

A

L

H

S

T

E

E

Q

E

D

D

I

I

A

D

D

A

T

T

V

L

A

A

A

A

A

A

K

R

K

T

V

V

M

M

A

S

T

A

L

L

active site: V21 (= V19), Y144 (= Y142), D239 (= D237), M242 (= M240), K267 (= K265), A401 (= A400)
binding (4s)-4,5-diaminopentanoic acid: G298 (= G296), T299 (= T297)
binding (4r)-5-amino-4-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]pentanoic acid: S23 (= S21), V25 (= V23), W61 (= W59), S116 (= S114), G117 (= G115), T118 (= T116), C121 (≠ T119), Y144 (= Y142), H145 (= H143), G146 (= G144), E206 (= E204), N211 (= N209), D239 (= D237), V241 (= V239), M242 (= M240), K267 (= K265), E400 (= E399)
binding pyridoxal-5'-phosphate: G298 (= G296), T299 (= T297)

2hp1A Inter-subunit signaling in gsam (see paper)
54% identity, 99% coverage: 4:426/426 of query aligns to 6:427/427 of 2hp1A

query
sites
2hp1A

S

S

E

D

T

E

L

I

F

F

E

A

Q

A

A

A

Q

Q

K

K

Y

L

I

M

P

P

G

G

G

G

V
|
V

N

S

S
|
S

P

P

V
|
V

R

R

A

A

F

F

R

K

G

S

V

V

G

G

G

G

T

Q

P

P

I

I

F

V

F

F

K

D

H

R

A

V

Q

K

G

D

A

A

Y

Y

L

A

Y

W

D

D

E

V

D

D

D

G

Q

N

R

R

Y

Y

I

I

D

D

Y

Y

I

V

G

G

S

T

W
|
W

G

G

P

P

M

A

I

I

L

C

G

G

H

H

G

A

D

H

Q

P

R

E

I

V

K

I

D

E

A

A

L

L

H

K

A

V

Q

A

V

M

D

E

L

K

G

G

V

T

G

S

Y

F

G
|
G

A

A

P

P

T

C

A

A

L

L

E

E

T

N

E

V

M

L

A

A

K

E

K

M

V

V

C

N

E

D

L

A

M

V

P

P

S

S

I

I

E

E

L

M

V

V

R

R

M

F

V

V

N

N

S
|
S

G
|
G

T
|
T

E

E

A

A

T
x
C

M

M

S

A

T

V

V

L

R

R

L

I

A

M

R

R

G

A

Y

Y

T

T

G

G

R

R

D

D

K

K

I

I

V

I

K

K

F

F

E

E

G

G

C

C

Y
|
Y

H
|
H

G
|
G

H

H

V

A

D

D

S

M

L

F

L

L

V

V

K

K

A

A

G

G

S

S

G

G

A

V

L

A

T

T

L

L

G

G

V

L

P

P

N

S

S

S

P

P

G

G

I

V

P

P

A

K

S

K

L

T

A

T

E

A

H

N

T

T

I

L

T

T

L

T

T

P

Y

Y

N

N

D

D

I

L

D

E

S

A

V

V

R

K

E

A

C

L

F

F

R

A

E

E

M

N

G

P

D

G

Q

E

I

I

A

A

A

G

I

V

I

I

V

L

E
|
E

P

P

V

I

A

V

G

G

N
|
N

M

S

N

G

C

F

I

I

P

V

P

P

V

D

P

A

G

G

F

F

L

L

E

E

G

G

L

L

R

R

E

E

V

I

C

T

D

L

E

E

H

H

G

D

T

A

V

L

L

L

I

V

F

F

D
|
D

E

E

V
|
V

M
|
M

T

T

G

G

F

F

R

R

V

I

S

A

L

Y

G

G

G

G

A

V

Q

Q

G

E

L

K

Y

F

G

G

V

V

T

T

P

P

D

D

L

L

T

T

A

T

L

L

G

G

K
|
K

V

I

I

I

G

G

G

G

G

G

L

L

P

P

V

V

G

G

A

A

F

Y

G

G

G

G

K

K

R

R

E

E

I

I

M

M

E

Q

H

L

I

V

S

A

P

P

L

A

G

G

P

P

V

M

Y

Y

Q

Q

A
|
A

G
|
G

T
|
T

L

L

S

S

G

G

N

N

P

P

L

L

A

A

M

M

C

T

A

A

G

G

L

I

T

K

T

T

L

L

N

E

A

L

I

L

S

R

E

Q

P

P

G

G

F

T

H

Y

D

E

R

Y

L

L

T

D

E

Q

K

I

T

T

N

K

A

R

V

L

R

S

D

D

G

G

L

L

K

L

E

A

A

I

A

A

D

Q

A

E

T

T

G

G

I

H

P

A

L

A

T

C

V

G

Q

G

S

Q

A

V

G

S

A

G

M

M

F

F

G

G

F

F

F

F

F

F

T

T

E

-

E

E

S

G

S

P

V

V

T

H

R

N

F

Y

D

E

Q

D

V

A

M

K

G

K

C

S

D

D

V

L

E

Q

R

K

F

F

K

S

K

R

F

F

F

H

Q

R

G

G

M

M

L

L

K

E

E

Q

G

G

V

I

Y

Y

L

L

A

A

P

P

S

S

A

Q

F

F

E
|
E

A
|
A

G

G

F

F

T

T

T

S

A

L

A

A

L

H

S

T

E

E

Q

E

D

D

I

I

A

D

D

A

T

T

V

L

A

A

A

A

A

A

K

R

K

T

V

V

M

M

A

S

T

A

L

L

active site: V21 (= V19), Y144 (= Y142), D239 (= D237), M242 (= M240), K267 (= K265), A401 (= A400)
binding (4s)-4-amino-5-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]pentanoic acid: S23 (= S21), V25 (= V23), W61 (= W59), S116 (= S114), G117 (= G115), T118 (= T116), C121 (≠ T119), Y144 (= Y142), H145 (= H143), G146 (= G144), E206 (= E204), N211 (= N209), D239 (= D237), V241 (= V239), M242 (= M240), K267 (= K265), E400 (= E399)
binding (4s)-4,5-diaminopentanoic acid: G88 (= G86), A297 (= A295), G298 (= G296), T299 (= T297)
binding pyridoxal-5'-phosphate: G298 (= G296), T299 (= T297)

2hozA Inter-subunit signaling in gsam (see paper)
54% identity, 99% coverage: 4:426/426 of query aligns to 6:427/427 of 2hozA

query
sites
2hozA

S

S

E

D

T

E

L

I

F

F

E

A

Q

A

A

A

Q

Q

K

K

Y

L

I

M

P

P

G

G

G

G

V
|
V

N

S

S

S

P

P

V

V

R

R

A

A

F

F

R

K

G

S

V

V

G

G

G

G

T

Q

P

P

I

I

F

V

F

F

K

D

H

R

A

V

Q

K

G

D

A

A

Y

Y

L

A

Y

W

D

D

E

V

D

D

D

G

Q

N

R

R

Y

Y

I

I

D

D

Y

Y

I

V

G

G

S

T

W

W

G

G

P

P

M

A

I

I

L

C

G

G

H

H

G

A

D

H

Q

P

R

E

I

V

K

I

D

E

A

A

L

L

H

K

A

V

Q

A

V

M

D

E

L

K

G

G

V

T

G

S

Y

F

G

G

A

A

P

P

T

C

A

A

L

L

E

E

T

N

E

V

M

L

A

A

K

E

K

M

V

V

C

N

E

D

L

A

M

V

P

P

S

S

I

I

E

E

L

M

V

V

R

R

M

F

V

V

N

N

S
|
S

G
|
G

T
|
T

E

E

A

A

T
x
C

M

M

S

A

T

V

V

L

R

R

L

I

A

M

R

R

G

A

Y

Y

T

T

G

G

R

R

D

D

K

K

I

I

V

I

K

K

F

F

E
|
E

G

G

C

C

Y
|
Y

H
|
H

G
|
G

H

H

V

A

D

D

S

M

L

F

L

L

V

V

K

K

A

A

G
|
G

S
|
S

G

G

A

V

L

A

T

T

L

L

G

G

V

L

P

P

N

S

S

S

P

P

G

G

I

V

P

P

A

K

S

K

L

T

A

T

E

A

H

N

T

T

I

L

T

T

L

T

T
x
P

Y

Y

N

N

D

D

I

L

D

E

S

A

V

V

R

K

E

A

C

L

F

F

R

A

E

E

M

N

G

P

D

G

Q

E

I

I

A

A

A

G

I

V

I

I

V

L

E
|
E

P

P

V

I

A

V

G

G

N
|
N

M

S

N

G

C

F

I

I

P

V

P

P

V

D

P

A

G

G

F

F

L

L

E

E

G

G

L

L

R

R

E

E

V

I

C

T

D

L

E

E

H

H

G

D

T

A

V

L

L

L

I

V

F

F

D
|
D

E

E

V
|
V

M
|
M

T

T

G

G

F

F

R

R

V

I

S

A

L

Y

G

G

G

G

A

V

Q

Q

G

E

L

K

Y

F

G

G

V

V

T

T

P

P

D

D

L

L

T

T

A

T

L

L

G

G

K
|
K

V

I

I

I

G

G

G

G

G

G

L

L

P

P

V

V

G

G

A

A

F

Y

G

G

G

G

K

K

R

R

E

E

I

I

M

M

E

Q

H

L

I

V

S

A

P

P

L

A

G

G

P

P

V

M

Y

Y

Q

Q

A

A

G
|
G

T
|
T

L

L

S

S

G

G

N

N

P

P

L

L

A

A

M

M

C

T

A

A

G

G

L

I

T

K

T

T

L

L

N

E

A

L

I

L

S

R

E

Q

P

P

G

G

F

T

H

Y

D

E

R

Y

L

L

T

D

E

Q

K

I

T

T

N

K

A

R

V

L

R

S

D

D

G

G

L

L

K

L

E

A

A

I

A

A

D

Q

A

E

T

T

G

G

I

H

P

A

L

A

T

C

V

G

Q

G

S

Q

A

V

G

S

A

G

M

M

F

F

G

G

F

F

F

F

F

F

T

T

E

-

E

E

S

G

S

P

V

V

T

H

R
x
N

F

Y

D
x
E

Q

D

V

A

M
x
K

G

K

C

S

D

D

V

L

E

Q

R

K

F

F

K

S

K

R

F

F

F

H

Q

R

G

G

M

M

L

L

K

E

E

Q

G

G

V

I

Y

Y

L

L

A

A

P

P

S

S

A

Q

F

F

E

E

A
|
A

G

G

F

F

T

T

T

S

A

L

A

A

L

H

S

T

E

E

Q

E

D

D

I

I

A

D

D

A

T

T

V

L

A

A

A

A

A

A

K

R

K

T

V

V

M

M

A

S

T

A

L

L

active site: V21 (= V19), Y144 (= Y142), D239 (= D237), M242 (= M240), K267 (= K265), A401 (= A400)
binding (4s)-4,5-diaminopentanoic acid: E141 (= E139), G156 (= G154), S157 (= S155), P182 (≠ T180), N368 (≠ R367), E370 (≠ D369), K373 (≠ M372)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: S116 (= S114), G117 (= G115), T118 (= T116), C121 (≠ T119), Y144 (= Y142), H145 (= H143), G146 (= G144), E206 (= E204), N211 (= N209), D239 (= D237), V241 (= V239), M242 (= M240), K267 (= K265), G298 (= G296), T299 (= T297)

3fqaA Gabaculien complex of gabaculine resistant gsam version (see paper)
54% identity, 99% coverage: 4:426/426 of query aligns to 5:426/426 of 3fqaA

query
sites
3fqaA

S

S

E

D

T

E

L

I

F

F

E

A

Q

A

A

A

Q

Q

K

K

Y

L

I

M

P

P

G

G

G

G

V
|
V

N

S

S
|
S

P

P

V

V

R
|
R

A

A

F

F

R

K

G

S

V

V

G

G

G

G

T

Q

P

P

I

I

F

V

F

F

K

D

H

R

A

V

Q

K

G

D

A

A

Y

Y

L

A

Y

W

D

D

E

V

D

D

D

G

Q

N

R

R

Y

Y

I

I

D

D

Y

Y

I

V

G

G

S

T

W
|
W

G

G

P

P

M

A

I

I

L

C

G

G

H

H

G

A

D

H

Q

P

R

E

I

V

K

I

D

E

A

A

L

L

H

K

A

V

Q

A

V

M

D

E

L

K

G

G

V

T

G

S

Y

F

G

G

A

A

P

P

T

C

A

A

L

L

E

E

T

N

E

V

M

L

A

A

K

E

K

M

V

V

C

N

E

D

L

A

M

V

P

P

S