SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF3532 FitnessBrowser__WCS417:GFF3532 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P07821 Iron(3+)-hydroxamate import ATP-binding protein FhuC; Ferric hydroxamate uptake protein C; Ferrichrome transport ATP-binding protein FhuC; Iron(III)-hydroxamate import ATP-binding protein FhuC; EC 7.2.2.16 from Escherichia coli (strain K12) (see 2 papers)
44% identity, 90% coverage: 18:255/263 of query aligns to 26:264/265 of P07821

query
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P07821

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K50 (= K42) mutation to Q: Lack of activity.
D172 (= D164) mutation to E: Lack of activity.
E173 (= E165) mutation to A: Lack of activity.

4u00A Crystal structure of ttha1159 in complex with adp (see paper)
30% identity, 86% coverage: 3:228/263 of query aligns to 2:225/241 of 4u00A

query
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4u00A

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binding adenosine-5'-diphosphate: F12 (≠ Y13), V17 (≠ I18), S37 (≠ N38), G38 (= G39), S39 (≠ C40), G40 (= G41), K41 (= K42), S42 (= S43), T43 (= T44)

P0AAH0 Phosphate import ATP-binding protein PstB; ABC phosphate transporter; Phosphate-transporting ATPase; EC 7.3.2.1 from Escherichia coli (strain K12) (see paper)
32% identity, 89% coverage: 2:235/263 of query aligns to 9:248/257 of P0AAH0

query
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P0AAH0

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G48 (= G41) mutation to I: Loss of phosphate transport.
K49 (= K42) mutation to Q: Loss of phosphate transport.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

P21439 Phosphatidylcholine translocator ABCB4; ATP-binding cassette sub-family B member 4; Multidrug resistance protein 3; P-glycoprotein 3; EC 7.6.2.1 from Homo sapiens (Human) (see 22 papers)
28% identity, 83% coverage: 14:230/263 of query aligns to 407:621/1286 of P21439

query
sites
P21439

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GCGKST 432:437 (= GCGKST 39:44) binding
K435 (= K42) mutation to M: Inhibits efflux activity for PC and cholesterol, but does not alter glycosylation and surface expression in the presence of taurocholate.
E450 (≠ Q57) to G: in dbSNP:rs1189003716
D459 (≠ A66) to H: in PFIC3; retained in the reticulum endoplasmic; greatly reduced expression
Q477 (= Q84) binding
P479 (= P87) to L: in PFIC3; greatly reduced expression; alters efflux activity for PC; dbSNP:rs748657435
L481 (≠ P89) to R: in PFIC3; does not alter cytoplasmic and cell membrane location; inhibits efflux activity for PC and cholesterol
A511 (≠ V120) to T: in PFIC3 and GBD1; dbSNP:rs1257887155
E528 (≠ R135) to D: in GBD1; uncertain significance; moderate decrease of phosphatidylcholine transporter activity; does not alter plasma membrane location; dbSNP:rs8187797
G535 (= G142) to D: in PFIC3; reduced phosphatidylcholine transporter activity; does not alter plasma membrane location
G536 (= G143) binding ; to R: in GBD1; loss of phosphatidylcholine transporter activity; does not alter plasma membrane location
I541 (≠ A148) to F: in PFIC3 and GBD1; dbSNP:rs66904256
A546 (≠ V153) to D: in ICP3; disruption of protein trafficking with subsequent lack of functional protein at the cell surface; dbSNP:rs121918441
E558 (= E165) mutation to Q: Loss of floppase activity. Strongly reduce the ATPase activity.
H589 (= H197) to T: in GBD1; requires 2 nucleotide substitutions
R590 (≠ D198) to Q: found in patients with gallbladder and cholestasis; uncertain significance; dbSNP:rs45575636

Sites not aligning to the query:

34 modified: Phosphothreonine; T → M: in GBD1; reduces efflux activity for PC in a phosphorylation-dependent manner; dbSNP:rs142794414; T→D: Does not inhibit efflux activity for PC.
44 T→A: Reduces efflux activity for PC. Does not alter apical membrane location.
47 R → G: in GBD1; partly retained intracellularly; reduces efflux activity for PC in a phosphorylation-dependent manner; R → Q: found in patients with cholangitis; uncertain significance; dbSNP:rs372685632
49 S→A: Reduces efflux activity for PC. Does not alter apical membrane location.
68 G → R: in PFIC3; retained in the reticulum endoplasmic; greatly reduced expression; dbSNP:rs1343667900
73 L → V: in PFIC3 and GBD1; dbSNP:rs8187788
87 natural variant: D -> E
95 P → S: in dbSNP:rs377268767
175 T → A: found in patients with gallbladder and cholestasis; uncertain significance; dbSNP:rs58238559
201 T → M: in PFIC3; greatly reduced expression; alters efflux activity for PC; dbSNP:rs753318087
238 L → V: in dbSNP:rs45596335
263 I → V: in dbSNP:rs45547936
286 A → V: in PFIC3 and GBD1; does not alter plasma membrane location; inhibits efflux activity for PC; dbSNP:rs765478923
320 S → F: in ICP3, GBD1 and PFIC3; uncertain significance; does not alter plasma membrane location; does not inhibit efflux activity for PC; dbSNP:rs72552778
367 I → V: in dbSNP:rs1168923653
403 Y → H: in PFIC3; does not alter cytoplasmic and cell membrane location; inhibits efflux activity for PC and cholesterol; dbSNP:rs121918443
406 binding ; R → Q: found in patients with cholangitis; uncertain significance; dbSNP:rs763807769
651 T → N: in dbSNP:rs45476795
652 R → G: in dbSNP:rs2230028
726 P → L: in GBD1; loss of phosphatidylcholine transporter activity; does not alter plasma membrane location; dbSNP:rs141677867
742 natural variant: G -> S
764 I → L: in a heterozygous patient with risperidone-induced cholestasis
775 T → M: found in patients with cholangitis; uncertain significance; dbSNP:rs148052192
788 R → Q: in GBD1; benign; dbSNP:rs8187801
934 A → T: found in patients with gallbladder and cholestasis; uncertain significance; dbSNP:rs61730509
953 A→D: Accumulates predominantly in intracellular compartments with only a small fraction at the plasma membrane and inhibits partially the efflux activity for PC.
964 V → T: found in patients with cholangitis; uncertain significance; requires 2 nucleotide substitutions
978 S → P: in PFIC3; alters efflux activity for PC; dbSNP:rs1051861187
985 V→M: Significantly reduces phosphatidylcholine floppase activity; when associated with Q-989 and V-990.
989 H→Q: Significantly reduces phosphatidylcholine floppase activity; when associated with M-985 and V-990.
990 A→V: Significantly reduces phosphatidylcholine floppase activity; when associated with M-985 and Q-989.
1046 binding
1071:1077 binding
1075 K→M: Inhibits efflux activity for PC and cholesterol, but does not alter glycosylation and surface expression in the presence of taurocholate.
1082 L → Q: in a heterozygous patient with amoxicillin/clavulanic acid-induced cholestasis; dbSNP:rs1214110864
1124 binding
1125 E → K: in PFIC3; alters efflux activity for PC
1168 P → S: in GBD1; reduced phosphatidylcholine transporter activity; does not alter plasma membrane location; dbSNP:rs121918442
1183 S → L: in GBD1; severely reduced phosphatidylcholine transporter activity; does not alter plasma membrane location
1184:1186 binding
1185 G → S: in GBD1; loss of phosphatidylcholine transporter activity; does not alter plasma membrane location

Q9JI39 ATP-binding cassette sub-family B member 10, mitochondrial; ABC-mitochondrial erythroid protein; ABC-me protein; ATP-binding cassette transporter 10; ABC transporter 10 protein from Mus musculus (Mouse) (see 2 papers)
33% identity, 78% coverage: 18:221/263 of query aligns to 474:678/715 of Q9JI39

query
sites
Q9JI39

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L

D

D

G

G

H

H

A

D

Y

I

T

R

S

Q

L

L

S

N

A

P

R

V

Q

W

L

L

A

R

R

S

Q

K

I

I

A

G

F

T

L

V

P

S

Q

Q

V

E

L

-

P

P

V

V

P

L

E

F

G

S

V
x
C

S

S

V

V

R

A

Q

E

L

N

V

I

A

A

Y

Y

G

G

R

A

S

D

P

N

H

L

N

S

S

S

L

V

W

T

G

A

R

Q

L

-

S

Q

G

V

N

E

D

R

Q

A

A

A

H

E

V

V

T

A

Q

N

A

A

M

A

Q

E

R

F

L

I

E

R

-

S

-

F

-

P

-

Q

-

G

L

F

D

D

T

T

L

V

A

V

D

G

R

E

A

K

L

G

A

I

D

L

L

L

S

S

G

G

G
|
G

Q

Q

R

K

Q

Q

R

R

A

I

W

A

L

I

A

A

M

R

V

A

L

L

A

L

Q

K

N

N

A

P

P

K

V

I

V

L

L

L

D

D

E
|
E

P

A

T

T

T

S

Y

A

L

L

D

D

I

A

S

E

H

N

Q

E

V

H

E

L

L

V

L

Q

D

E

L

A

M

L

G

D

E

R

L

L

A

M

G

-

E

E

G

G

K

R

T

T

V

V

I

L

T

I

V

I

L

A

H

H

D

R

I

L

N

S

Q

-

A

T

C

I

R

K

Y

N

A

A

H

N

H

F

L

V

A

A

V

V

M

L

H

D

G

H

G

G

K

K

L

I

V
x
C

A

E

D

H

G

G

G497 (= G41) mutation to A: Decreases ATP binding about 50%.
K498 (= K42) mutation to R: Decreases ATP binding about 50%.
C547 (≠ V92) modified: S-glutathionyl cysteine; mutation to A: Does not affect ABCB10 glutathionylation.
G602 (= G143) mutation to D: Affects ATP hydrolysis but not binding.; mutation to V: Affects ATP hydrolysis but not binding.
E624 (= E165) mutation to Q: Affects ATP hydrolysis but not binding.
C675 (≠ V218) mutation to A: Prevents ABCB10 glutathionylation.

Sites not aligning to the query:

1:82 modified: transit peptide, Mitochondrion

5x40A Structure of a cbio dimer bound with amppcp (see paper)
33% identity, 86% coverage: 3:228/263 of query aligns to 4:229/280 of 5x40A

query
sites
5x40A

I

I

L

L

Q

A

A

A

R

E

E

A

L

L

D

T

I

Y

G

A

Y
x
F

-

P

G

G

T

G

T
x
V

R

K

I
x
A

V

L

Q

D

G

D

L

L

S

S

F

L

V

A

P

V

P

P

T

K

G

G

K

E

V

S

T

L

A

A

L

I

I

L

G

G

P

P

N
|
N

G
|
G

C

A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

K

L

A

H

F

L

A

N

R

G

I

T

L

L

K

R

P

P

T

Q

Q

S

G

G

E

R

L

V

T

L

L

L

D

G

G

G

H

T

A

A

Y

-

T

T

S

G

L

H

S

S

A

R

R

K

Q

D

L

L

A

T

-

G

-

W

-

R

R

R

Q

R

I

V

A

G

F

L

L

V

P

L

Q
|
Q

V

D

L

A

P

D

V

D

P

Q

-

L

E

F

G

A

V

T

S

T

V

V

R

F

Q

E

L

D

V

V

A

S

Y

F

G

G

R

-

S

-

P

P

H

L

N

N

S

-

L

-

W

L

G

G

R

L

L

S

S

E

G

A

N

E

D

A

Q

R

A

A

H

R

V

V

T

E

Q

E

A

A

M

L

Q

A

R

A

L

L

E

S

L

I

D

S

T

D

L

L

A

R

D

D

R

R

A

P

L

T

A
x
H

D
x
M

L
|
L

S
|
S

G

G

G
|
G

Q
|
Q

R

K

Q

R

R

R

A

V

W

A

L

I

A

A

M

G

V

A

L

V

A

A

Q

M

N

R

A

P

P

E

V

V

V

L

L

L

D

D

E
x
Q

P

P

T

T

T

A

Y

G

L

L

D

D

I

L

S

A

H

G

Q

T

V

E

E

Q

L

L

D

T

L

L

M

L

G

R

E

G

L

L

A

R

G

A

E

A

G

G

K

M

T

T

V

L

I

V

T

F

V

S

L

T

H
|
H

D

D

I

V

N

E

Q

L

A

A

C

A

R

A

Y

L

A

A

H

D

H

R

L

V

A

A

V

L

M

F

H

R

G

T

G

G

K

R

L

V

V

L

A

A

D

E

G

G

A

A

P

A

G

E

Q

A

V

V

I

L

T

S

binding phosphomethylphosphonic acid adenylate ester: F14 (≠ Y13), V18 (≠ T16), A20 (≠ I18), N40 (= N38), G41 (= G39), G43 (= G41), K44 (= K42), S45 (= S43), T46 (= T44), Q88 (= Q84), H139 (≠ A138), M140 (≠ D139), L141 (= L140), S142 (= S141), G144 (= G143), Q145 (= Q144), Q166 (≠ E165), H198 (= H197)
binding magnesium ion: S45 (= S43), Q88 (= Q84)

4f4cA The crystal structure of the multi-drug transporter (see paper)
30% identity, 80% coverage: 18:228/263 of query aligns to 1038:1248/1250 of 4f4cA

query
sites
4f4cA

I

I

V

L

Q

K

G

G

L

L

S

S

F

F

V

S

P

V

P

E

T

P

G

G

K

Q

V

T

T

L

A

A

L

L

I

V

G

G

P

P

N

S

G

G

C

C

G

G

K

K

S

S

T

T

L

V

K

A

A

L

F

L

A

E

R

R

I

F

L

Y

K

D

P

T

T

L

Q

G

G

G

E

E

L

I

T

F

L

I

D

D

G

G

H

S

A

E

Y

I

T

K

S

T

L

L

S

N

A

P

R

E

Q

H

L

T

A

R

R

S

Q

Q

I

I

A

A

F

I

L

V

P

S

Q

Q

V

E

L

-

P

P

V

T

P

L

E

F

G

D

V

C

S

S

V

I

R

A

Q

E

L

N

V

I

A

I

Y

Y

G

G

R

L

S

D

P

P

H

S

N

S

S

V

L

T

W

M

G

A

R

Q

L

V

S

E

-

E

-

A

-

R

-

L

G

A

N

N

D

I

Q

H

A

N

H

F

V

I

T

A

Q

E

A

L

M

P

Q

E

R

G

L

F

E

E

L

-

D

T

T

R

L

V

A

G

D

D

R

R

A

G

L

-

A

T

D

Q

L

L

S

S

G

G

Q

Q

R

K

Q

Q

R

R

A

I

W

A

L

I

A

A

M

R

V

A

L

L

A

V

Q

R

N

N

A

P

P

K

V

I

V

L

L

L

D

D

E

E

P

A

T

T

T

S

Y

A

L

L

D

D

I

T

S

E

H

S

Q

E

V

K

E

V

L

V

L

Q

D

E

L

A

M

L

G

-

E

D

L

R

A

A

G

R

E

E

G

G

K

R

T

T

V

C

I

I

T

V

V

I

L

A

H

H

D

R

I

L

N

N

Q

T

A

V

C

M

R

N

Y

-

A

A

H

D

H

C

L

I

A

A

V

V

M

V

H

S

G

N

G

G

K

T

L

I

V

I

A

E

D

K

G

G

A

T

P

H

G

T

Q

Q

V

L

I

M

T

S

Sites not aligning to the query:

binding undecyl 4-O-alpha-D-glucopyranosyl-1-thio-beta-D-glucopyranoside: 19, 857, 857, 858, 860, 934, 973, 976, 976, 979

4ayxA Structure of the human mitochondrial abc transporter, abcb10 (rod form b) (see paper)
32% identity, 78% coverage: 18:221/263 of query aligns to 356:560/571 of 4ayxA

query
sites
4ayxA

I

I

V

F

Q

Q

G

D

L

F

S

S

F

L

V

S

P

I

P

P

T

S

G

G

K

S

V

V

T

T

A

A

L

L

I

V

G

G

P

P

N
x
S

G
|
G

C

S

G
|
G

K
|
K

S
|
S

T
|
T

L

V

L

L

K

S

A

L

F

L

A

L

R

R

I

L

L

Y

K

D

P

P

T

A

Q

S

G

G

E

T

L

I

T

S

L

L

D

D

G

G

H

H

A

D

Y

I

T

R

S

Q

L

L

S

N

A

P

R

V

Q

W

L

L

A

R

R

S

Q

K

I

I

A

G

F

T

L

V

P

S

Q
|
Q

V

E

L

-

P

P

V

I

P

L

E

F

G

S

V

C

S

S

V

I

R

A

Q

E

L

N

V

I

A

A

Y

Y

G

G

R

A

S

D

P

D

H

P

N

S

S

S

L

V

W

T

G

A

R

E

L

-

S

E

G

I

N

Q

D

R

Q

V

A

A

H

E

V

V

T

A

Q

N

A

A

M

V

Q

A

R

F

L

I

E

R

-

N

-

F

-

P

-

Q

-

G

L

F

D

N

T

T

L

V

A

V

D

G

R

E

A

K

L

G

A

V

D

L

L

L

S

S

G

G

Q

Q

R

K

Q

Q

R

R

A

I

W

A

L

I

A

A

M

R

V

A

L

L

A

L

Q

K

N

N

A

P

P

K

V

I

V

L

L

L

D

D

E

E

P

A

T

T

T

S

Y

A

L

L

D

D

I

A

S

E

H

N

Q

E

V

Y

E

L

L

V

L

Q

D

E

L

A

M

L

G

D

E

R

L

L

A

M

G

-

E

D

G

G

K

R

T

T

V

V

I

L

T

V

V

I

L

A

H

H

D

R

I

L

N

S

Q

-

A

T

C

I

R

K

Y

N

A

A

H

N

H

M

L

V

A

A

V

V

M

L

H

D

G

Q

G

G

K

K

L

I

V

T

A

E

D

Y

G

G

binding phosphomethylphosphonic acid adenylate ester: S376 (≠ N38), G377 (= G39), G379 (= G41), K380 (= K42), S381 (= S43), T382 (= T44)
binding magnesium ion: S381 (= S43), Q422 (= Q84)

Sites not aligning to the query:

7niwA Nanodisc reconstituted human abcb4 in complex with 4b1-fab (posaconazole-bound, inward-open conformation) (see paper)
28% identity, 83% coverage: 14:230/263 of query aligns to 356:570/1140 of 7niwA

query
sites
7niwA

G

A

T

N

T

V

R

K

I

I

V

L

Q

K

G

G

L

L

S

N

F

L

V

K

P

V

P

Q

T

S

G

G

K

Q

V

T

T

V

A

A

L

L

I

V

G

G

P

S

N

S

G

G

C

C

G

G

K

K

S

S

T

T

L

T

L

V

K

Q

A

L

F

I

A

Q

R

R

I

L

L

Y

K

D

P

P

T

D

Q

E

G

G

E

T

L

I

T

N

L

I

D

D

G

G

H

Q

A

D

Y

I

T

R

S

N

L

F

S

N

A

V

R

N

Q

Y

L

L

A

R

R

E

Q

I

I

I

A

G

F

V

L

V

P

S

Q

Q

V

E

L

-

P

P

V

V

P

L

E

F

G

S

V

T

S

T

V

I

R

A

Q

E

L

N

V

I

A

C

Y

Y

G

G

R

R

S

-

P

-

H

-

N

-

S

-

L

-

W

-

G

G

R

N

L

V

S

T

G

M

N

D

D

E

-

I

-

K

-

A

-

V

-

K

Q

E

A

A

H

N

V

A

T

Y

Q

E

A

F

M

I

Q

M

R

K

L

L

-

P

-

Q

E

K

L

F

D

D

T

T

L

L

A

V

D

G

R

E

A

R

L

G

A

A

D

Q

L

L

S

S

G

G

Q

Q

R

K

Q

Q

R

R

A

I

W

A

L

I

A

A

M

R

V

A

L

L

A

V

Q

R

N

N

A

P

P

K

V

I

V

L

L

L

D

D

E

Q

P

A

T

T

T

S

Y

A

L

L

D

D

I

T

S

E

H

S

Q

E

V

A

E

E

L

V

L

Q

D

A

L

A

M

L

G

D

E

K

L

-

A

A

G

R

E

E

G

G

K

R

T

T

V

T

I

I

T

V

V

I

L

A

H

H

D

R

I

L

N

S

Q

-

A

T

C

V

R

R

Y

N

A

A

H

D

H

V

L

I

A

A

V

G

M

F

H

E

G

D

G

G

K

V

L

I

V

V

A

E

D

Q

G

G

A

S

P

H

G

S

Q

E

V

L

I

M

T

K

A

K

E

E

Sites not aligning to the query:

binding cholesterol: 70, 152, 160, 266, 641, 645, 648, 839
binding 1,2-dilinoleoyl-sn-glycero-3-phosphocholine: 173, 183, 250, 254, 257, 261, 286, 294, 298, 617, 882
binding posaconazole: 250, 291, 294, 658, 661, 726, 841, 871, 874, 878, 882

6s7pA Nucleotide bound abcb4 (see paper)
28% identity, 83% coverage: 14:230/263 of query aligns to 346:560/1128 of 6s7pA

query
sites
6s7pA

G

A

T

N

T

V

R

K

I

I

V

L

Q

K

G

G

L

L

S

N

F

L

V

K

P

V

P

Q

T

S

G

G

K

Q

V

T

T

V

A

A

L

L

I

V

G

G

P

S

N

S

G
|
G

C

C

G
|
G

K
|
K

S
|
S

T

T

L

T

L

V

K

Q

A

L

F

I

A

Q

R

R

I

L

L

Y

K

D

P

P

T

D

Q

E

G

G

E

T

L

I

T

N

L

I

D

D

G

G

H

Q

A

D

Y

I

T

R

S

N

L

F

S

N

A

V

R

N

Q

Y

L

L

A

R

R

E

Q

I

I

I

A

G

F

V

L

V

P

S

Q
|
Q

V

E

L

-

P

P

V

V

P

L

E

F

G

S

V

T

S

T

V

I

R

A

Q

E

L

N

V

I

A

C

Y

Y

G

G

R

R

S

-

P

-

H

-

N

-

S

-

L

-

W

-

G

G

R

N

L

V

S

T

G

M

N

D

D

E

-

I

-

K

-

A

-

V

-

K

Q

E

A

A

H

N

V

A

T

Y

Q

E

A

F

M

I

Q

M

R

K

L

L

-

P

-

Q

E

K

L

F

D

D

T

T

L

L

A

V

D

G

R

E

A

R

L

G

A

A

D
x
Q

L

L

S
|
S

G

G

G
|
G

Q
|
Q

R

K

Q

Q

R

R

A

I

W

A

L

I

A

A

M

R

V

A

L

L

A

V

Q

R

N

N

A

P

P

K

V

I

V

L

L

L

D

D

E

Q

P

A

T

T

T

S

Y

A

L

L

D

D

I

T

S

E

H

S

Q

E

V

A

E

E

L

V

L

Q

D

A

L

A

M

L

G

D

E

K

L

-

A

A

G

R

E

E

G

G

K

R

T

T

V

T

I

I

T

V

V

I

L

A

H

H

D

R

I

L

N

S

Q

-

A

T

C

V

R

R

Y

N

A

A

H

D

H

V

L

I

A

A

V

G

M

F

H

E

G

D

G

G

K

V

L

I

V

V

A

E

D

Q

G

G

A

S

P

H

G

S

Q

E

V

L

I

M

T

K

A

K

E

E

binding adenosine-5'-triphosphate: G371 (= G39), G373 (= G41), K374 (= K42), S375 (= S43), Q416 (= Q84), Q471 (≠ D139), S473 (= S141), G475 (= G143), Q476 (= Q144)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 342, 344, 345, 920, 922, 923, 948, 949, 951, 952, 953, 954, 994, 1033, 1053, 1054, 1056
binding cholesterol: 25, 32, 35, 65, 824, 834, 834, 838
binding magnesium ion: 953, 994

O65934 ABC transporter ATP-binding/permease protein Rv1747; EC 7.-.-.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 3 papers)
31% identity, 81% coverage: 14:226/263 of query aligns to 330:540/865 of O65934

query
sites
O65934

G

G

T

D

T

K

R

T

I

L

V

L

Q

D

G

G

L

I

S

S

F

L

V

T

P

A

P

R

T

P

G

G

K

M

V

L

T

T

A

A

L

V

I

I

G

G

P

P

N

S

G

G

C

A

G

G

K

K

S

S

T

T

L

L

L

A

K

R

A

L

F

V

A

A

R

G

I

Y

L

T

K

H

P

P

T

T

Q

D

G

G

E

T

L

V

T

T

L

F

D

E

G

G

H

H

-

N

-

V

-

H

-

A

A

E

Y

Y

T

A

S

S

L

L

S

R

A

S

R

R

Q

-

L

-

A

-

R

-

Q

-

I

I

A

G

F

M

L

V

P

P

Q

Q

V

D

L

D

P

V

V

V

P

H

E

G

G

Q

V

L

S

T

V

V

R

K

Q

H

L

A

V

L

A

M

Y

Y

G

A

-

A

-

E

-

L

R

R

S

L

P

P

H

P

N

D

S

T

L

-

W

-

G

-

R

-

L

-

S

T

G

K

N

D

D

D

Q

R

A

T

H

Q

V

V

T

V

-

A

Q

R

A

V

M

L

Q

E

R

E

L

L

E

E

L

M

D

S

T

K

L

H

A

I

D

D

R

T

A

R

L

V

A

D

D

K

L

L

S

S

G

G

Q

Q

R

R

Q

K

R

R

A

A

W

S

L

V

A

A

M

L

V

E

L

L

A

L

Q

T

N

G

A

P

P

S

V

L

L

I

L

L

D

D

E
|
E

P

P

T

T

T

S

Y

G

L

L

D

D

I

P

S

A

H

L

Q

D

V

R

E

Q

L

V

L

M

D

T

L

M

M

L

G

R

E

Q

L

L

A

A

G

D

E

A

G

G

K

R

T

V

V

V

I

L

T

V

V

V

L

T

H

H

D

S

I

L

N

T

Q

Y

A

L

C

D

R

V

Y

C

A

D

H

Q

H

V

L

L

A

L

V

L

M

A

H

P

G

G

K

K

L

T

V

A

A

F

D

C

G

G

A

P

P

P

G

T

Q

Q

V

I

E479 (= E165) mutation to Q: Loss of ATPase activity.

Sites not aligning to the query:

33 R→A: Strong decrease in phosphorylation.
47 S→A: Strong decrease in phosphorylation. Lack of interaction with PknF. Attenuates growth in macrophages.
69 N→A: Strong decrease in phosphorylation.
152 modified: Phosphothreonine; T→A: Lack of phosphorylation. Attenuates growth in macrophages and in mice; when associated with A-210.
210 modified: Phosphothreonine; T→A: Lack of phosphorylation. Attenuates growth in macrophages and in mice; when associated with A-152.
234 R→A: Strong decrease in phosphorylation.
248 S→A: Strong decrease in phosphorylation. Decreases interaction with PknF.
270 N→A: Strong decrease in phosphorylation.

5xu1B Structure of a non-canonical abc transporter from streptococcus pneumoniae r6 (see paper)
30% identity, 78% coverage: 17:222/263 of query aligns to 21:225/226 of 5xu1B

query
sites
5xu1B

R

Q

I

V

V

L

Q

K

G

N

L

I

S

N

F

L

V

E

P

V

P

N

T

E

G

G

K

E

V

F

T

V

A

A

L

I

I

M

G

G

P

P

N

S

G

G

C

S

G
|
G

K
|
K

S
|
S

T

T

L

L

L

M

K

N

A

T

F

I

A

G

R

M

I

L

L

D

K

T

P

P

T

T

Q

S

G

G

E

E

L

Y

T

Y

L

L

D

E

G

G

H

Q

A

E

Y

V

T

A

S

G

L

L

S

G

A

E

R

K

Q

Q

L

L

A

A

R

K

-

V

-

R

-

N

-

Q

Q

Q

I

I

A

G

F

F

L

V

P

F

Q

Q

V

Q

L

F

P

F

V

L

P

L

E

S

G

K

V

L

S

N

V

A

R

L

Q

Q

L

N

V

V

A

E

Y

L

G

-

R

-

S

-

P

P

H

L

N

-

S

-

L

I

W

Y

G

A

R

G

L

V

S

S

G

S

N

S

D

K

Q

R

A

R

H

K

V

L

T

A

Q

E

A

E

-

Y

M

L

Q

D

R

K

L

V

E

E

L

L

D

T

T

E

L

R

A

S

D

H

R

H

A

L

L

P

A

S

D

E

L

L

S

S

G

G

Q

Q

R

K

Q

Q

R

R

A

V

W

A

L

I

A

A

M

R

V

A

L

L

A

V

Q

N

N

N

A

P

P

S

V

I

L

L

L

A

D

D

E

E

P

P

T

T

T

G

Y

A

L

L

D

D

I

T

S

K

H

T

Q

G

V

N

E

Q

L

I

L

M

D

Q

L

L

M

L

G

V

E

D

L

L

A

N

G

K

E

E

G

G

K

K

T

T

V

I

I

I

T

M

V

V

L

T

H

H

D

E

I

P

N

E

Q

I

A

A

C

A

R

-

Y

Y

A

A

H

K

H

R

L

Q

A

I

V

V

M

I

H

R

G

D

G

G

K

V

L

I

V

S

A

S

D

D

G

S

A

A

binding magnesium ion: G45 (= G41), K46 (= K42), S47 (= S43)

Q9NRK6 ATP-binding cassette sub-family B member 10, mitochondrial; ABC-mitochondrial erythroid protein; ABC-me protein; ATP-binding cassette transporter 10; ABC transporter 10 protein; Mitochondrial ATP-binding cassette 2; M-ABC2 from Homo sapiens (Human) (see 5 papers)
32% identity, 78% coverage: 18:221/263 of query aligns to 509:713/738 of Q9NRK6

query
sites
Q9NRK6

I

I

V

F

Q

Q

G

D

L

F

S

S

F

L

V

S

P

I

P

P

T

S

G

G

K

S

V

V

T

T

A

A

L

L

I

V

G

G

P

P

N

S

G

G

C

S

G

G

K
|
K

S

S

T

T

L

V

L

L

K

S

A

L

F

L

A

L

R

R

I

L

L

Y

K
x
D

P

P

T

A

Q

S

G

G

E

T

L

I

T

S

L

L

D

D

G

G

H

H

A

D

Y

I

T

R

S

Q

L

L

S

N

A

P

R

V

Q

W

L

L

A

R

R

S

Q

K

I

I

A

G

F

T

L

V

P

S

Q

Q

V

E

L

-

P

P

V

I

P

L

E

F

G

S

V
x
C

S

S

V

I

R

A

Q

E

L

N

V

I

A

A

Y

Y

G

G

R

A

S

D

P

D

H

P

N

S

S

S

L

V

W

T

G

A

R

E

L

-

S

E

G

I

N

Q

D

R

Q

V

A

A

H

E

V

V

T

A

Q

N

A

A

M

V

Q

A

R

F

L

I

E

R

-

N

-

F

-

P

-

Q

-

G

L

F

D

N

T

T

L

V

A

V

D

G

R

E

A

K

L

G

A

V

D

L

L

L

S
|
S

G

G

Q
|
Q

R

K

Q

Q

R

R

A

I

W

A

L

I

A

A

M

R

V

A

L

L

A

L

Q

K

N

N

A

P

P

K

V

I

V

L

L

L

D
|
D

E
|
E

P

A

T

T

T

S

Y

A

L

L

D

D

I

A

S

E

H

N

Q

E

V

Y

E

L

L

V

L

Q

D

E

L

A

M

L

G

D

E

R

L

L

A

M

G

-

E

D

G

G

K

R

T

T

V

V

I

L

T

V

V

I

L

A

H

H

D

R

I

L

N

S

Q

-

A

T

C

I

R

K

Y

N

A

A

H

N

H

M

L

V

A

A

V

V

M

L

H

D

G

Q

G

G

K

K

L

I

V

T

A

E

D

Y

G

G

K533 (= K42) mutation to E: Increases hemoglobin biosynthetic process.
D545 (≠ K54) to N: in dbSNP:rs35698797
C582 (≠ V92) mutation to G: Does not affect ATPase activity; when associated with S-215 and L-224. Activated by Zn (II) mesoporphyrin; when associated with S-215 and L-224.
S635 (= S141) mutation to R: Does not rescue hemoglobin and heme biosynthetic process.
Q638 (= Q144) mutation to H: Does not rescue hemoglobin and heme biosynthetic process.
D658 (= D164) mutation to A: Does not rescue hemoglobin and heme biosynthetic process.
E659 (= E165) mutation to A: Does not rescue hemoglobin and heme biosynthetic process.

Sites not aligning to the query:

150 A → S: in dbSNP:rs4148756
215 C→S: Does not affect ATPase activity; when associated with L-224 and G-582. Activated by Zn (II) mesoporphyrin; when associated with L-224 and G-582.
224 C→L: Does not affect ATPase activity; when associated withS-215 and G-582. Activated by Zn (II) mesoporphyrin; when associated with S-215 and G-582.
471 R → T: in a breast cancer sample; somatic mutation

8hf4A Cryo-em structure of nucleotide-bound coma at outward-facing state with ec gate closed conformation (see paper)
31% identity, 80% coverage: 12:221/263 of query aligns to 340:545/563 of 8hf4A

query
sites
8hf4A

G

G

Y

Y

G

G

T

R

T

D

R

-

I

V

V

L

Q

S

G

D

L

I

S

N

F

L

V

T

P

V

P

P

T

Q

G

G

K

S

V

K

T

V

A

A

L

F

I

V

G

G

P

I

N
x
S

G
|
G

C
x
S

G
|
G

K
|
K

S
x
T

T
|
T

L

L

L

A

K

K

A

M

F

M

A

V

R

N

I

F

L

Y

K

D

P

P

T

S

Q

Q

G

G

E

E

L

I

T

S

L

L

D

G

G

G

H

V

A

N

Y

L

T

N

S

Q

L

I

S

D

A

K

R

K

Q

A

L

L

A

R

R

Q

Q

Y

I

I

A

N

F

Y

L

L

P

P

Q
|
Q

V

Q

L

P

P

Y

V

V

P

F

E

N

G

G

V

T

S

I

V

L

R

E

Q

N

L

L

V

L

A

L

Y

-

G

G

R

A

S

K

P

E

H

G

N

T

S

T

L

Q

W

E

G

D

R

I

L

L

S

R

G

A

N

V

D

E

Q

L

A

A

H

E

V

I

T

R

Q

E

A

D

M

I

Q

E

R

R

L

M

E

P

L

L

D

N

T

Y

L

Q

A

T

D

E

R

L

A

T

L

S

-

D

-

G

A

A

D

G

L

I

S

S

G

G

Q

Q

R

R

Q

Q

R

R

A

I

W

A

L

L

A

A

M

R

V

A

L

L

A

L

Q

T

N

D

A

A

P

P

V

V

V

I

L

I

L

L

D

D

E

E

P

A

T

T

T

S

Y

S

L

L

D

D

I

I

S

L

H

T

Q

E

V

K

E

R

L

I

L

V

D

D

L

-

M

-

G

-

E

N

L

L

A

I

G

A

E

L

G

D

K

K

T

T

V

L

I

I

T

F

V

I

L

A

H

H

D

R

I

L

N

T

Q

I

A

A

C

E

R

R

Y

-

A

T

H

E

H

K

L

V

A

V

V

V

M

L

H

D

G

Q

G

G

K

K

L

I

V

V

A

E

D

E

G

G

binding phosphothiophosphoric acid-adenylate ester: S365 (≠ N38), G366 (= G39), S367 (≠ C40), G368 (= G41), K369 (= K42), T370 (≠ S43), T371 (= T44), Q411 (= Q84)

Sites not aligning to the query:

binding phosphothiophosphoric acid-adenylate ester: 339

2ixfA Crystal structure of the atpase domain of tap1 with atp (d645q, q678h mutant) (see paper)
33% identity, 79% coverage: 15:221/263 of query aligns to 30:237/255 of 2ixfA

query
sites
2ixfA

T

N

T

V

R

Q

I
x
V

V

L

Q

Q

G

G

L

L

S

T

F

F

V

T

P

L

P

Y

T

P

G

G

K

K

V

V

T

T

A

A

L

L

I

V

G

G

P

P

N
|
N

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

V

L

A

K

A

A

L

F

L

A

Q

R

N

I

L

L

Y

K

Q

P

P

T

T

Q

G

G

G

E

K

L

V

T

L

L

L

D

D

G

G

H

E

A

P

Y

L

T

V

S

Q

L

Y

S

D

A

H

R

H

Q

Y

L

L

A

H

R

T

Q

Q

I

V

A

A

F

A

L

V

P

G

Q

Q

V

E

L

-

P

P

V

L

P

L

E

F

G

G

V

R

S

S

V

F

R

R

Q

E

L

N

V

I

A

A

Y

Y

G

G

-

L

-

T

R

R

S

T

P

P

H

T

N

M

S

E

L

E

W

I

G

T

R

A

L

V

S

A

G

M

N

E

D

S

Q

G

A

A

H

H

V

D

T

F

Q

I

A

S

M

G

Q

F

R

P

L

Q

E

G

L

Y

D

D

T

T

L

E

A

V

D

G

R

E

A

T

L

G

A
x
N

D

Q

L

L

S
|
S

G

G

G
|
G

Q
|
Q

R

R

Q

Q

R

A

A

V

W

A

L

L

A

A

M

R

V

A

L

L

A

I

Q

R

N

K

A

P

P

R

V

L

L

I

L

L

D

D

E

Q

P

A

T

T

T

S

Y

A

L

L

D

D

I

A

S

G

H

N

Q

Q

V

L

E

R

L

V

L

Q

D

R

L

L

M

L

G

Y

E

E

L

S

-

P

A

E

G

W

E

A

G

S

K

R

T

T

V

V

I

L

T

L

V

I

L

T

H
|
H

D

Q

I

L

N

S

Q

L

A

A

C

E

R

R

Y

-

A

A

H

H

L

I

A

L

V

F

M

L

H

K

G

E

G

G

K

S

L

V

V

C

A

E

D

Q

G

G

binding adenosine-5'-triphosphate: V33 (≠ I18), N53 (= N38), G54 (= G39), S55 (≠ C40), G56 (= G41), K57 (= K42), S58 (= S43), T59 (= T44), N154 (≠ A138), S157 (= S141), G159 (= G143), Q160 (= Q144), H214 (= H197)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 25

6q81A Structure of p-glycoprotein(abcb1) in the post-hydrolytic state (see paper)
30% identity, 83% coverage: 17:234/263 of query aligns to 372:582/1182 of 6q81A

query
sites
6q81A

R

Q

I

I

V

L

Q

K

G

G

L

L

S

N

F

L

V

K

P

V

P

K

T

S

G

G

K

Q

V

T

T

V

A

A

L

L

I

V

G

G

P

N

N

S

G
|
G

C

C

G
|
G

K
|
K

S
|
S

T

T

L

T

L

V

K

Q

A

L

F

M

A

Q

R

R

I

L

L

Y

K

D

P

P

T

L

Q

D

G

G

E

M

L

V

T

S

L

I

D

D

G

G

H

Q

A

D

Y

I

T

R

S

T

L

I

S

N

A

V

R

R

Q

Y

L

L

A

R

R

E

Q

I

I

I

A

G

F

V

L

V

P

S

Q

Q

V

E

L

-

P

P

V

V

P

L

E

F

G

A

V

T

S

T

V

I

R

A

Q

E

L

N

V

I

A

R

Y

Y

G

G

R

R

S

E

P

D

H

V

N

-

S

T

L

M

W

D

G

E

R

I

L

E

S

K

G

A

N

V

D

K

Q

E

A

A

H

N

V

A

T

Y

Q

D

A

F

M

I

Q

M

R

K

L

L

-

P

-

H

E

Q

L

F

D

D

T

T

L

L

A

V

D

G

R

E

A

R

L

G

A

A

D

Q

L

L

S

S

G

G

Q

Q

R

K

Q

Q

R

R

A

I

W

A

L

I

A

A

M

R

V

A

L

L

A

V

Q

R

N

N

A

P

P

K

V

I

V

L

L

L

D

D

E

E

P

A

T

T

T

S

Y

A

L

L

D

D

I

T

S

E

H

S

Q

E

V

A

E

V

L

V

L

Q

D

A

L

A

M

L

G

D

E

K

L

-

A

A

G

R

E

E

G

G

K

R

T

T

V

T

I

I

T

V

V

I

L

A

H

H

D

R

I

L

N

S

Q

-

A

T

C

V

R

R

Y

N

A

A

H

D

H

V

L

I

A

A

V

G

M

F

H

D

G

G

K

V

L

I

V

V

A

E

D

Q

G

G

A

N

P

H

G

D

Q

-

V

-

I

-

T

-

A

-

E

E

L

L

M

M

R

R

Q

E

binding adenosine-5'-diphosphate: G394 (= G39), G396 (= G41), K397 (= K42), S398 (= S43)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 365, 370, 712, 951, 979, 980, 982, 983, 984

7otgA Structure of abcb1/p-glycoprotein in the presence of the cftr potentiator ivacaftor (see paper)
30% identity, 83% coverage: 17:234/263 of query aligns to 372:582/1179 of 7otgA

query
sites
7otgA

R

Q

I

I

V

L

Q

K

G

G

L

L

S

N

F

L

V

K

P

V

P

K

T

S

G

G

K

Q

V

T

T

V

A

A

L

L

I

V

G

G

P

N

N

S

G

G

C

C

G

G

K

K

S

S

T

T

L

T

L

V

K

Q

A

L

F

M

A

Q

R

R

I

L

L

Y

K

D

P

P

T

L

Q

D

G

G

E

M

L

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T

S

L

I

D

D

G

G

H

Q

A

D

Y

I

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R

S

T

L

I

S

N

A

V

R

R

Q

Y

L

L

A

R

R

E

Q

I

I

I

A

G

F

V

L

V

P

S

Q

Q

V

E

L

-

P

P

V

V

P

L

E

F

G

A

V

T

S

T

V

I

R

A

Q

E

L

N

V

I

A

R

Y

Y

G

G

R

R

S

E

P

D

H

V

N

-

S

T

L

M

W

D

G

E

R

I

L

E

S

K

G

A

N

V

D

K

Q

E

A

A

H

N

V

A

T

Y

Q

D

A

F

M

I

Q

M

R

K

L

L

-

P

-

H

E

Q

L

F

D

D

T

T

L

L

A

V

D

G

R

E

A

R

L

G

A

A

D

Q

L

L

S

S

G

G

Q

Q

R

K

Q

Q

R

R

A

I

W

A

L

I

A

A

M

R

V

A

L

L

A

V

Q

R

N

N

A

P

P

K

V

I

V

L

L

L

D

D

E

E

P

A

T

T

T

S

Y

A

L

L

D

D

I

T

S

E

H

S

Q

E

V

A

E

V

L

V

L

Q

D

A

L

A

M

L

G

D

E

K

L

-

A

A

G

R

E

E

G

G

K

R

T

T

V

T

I

I

T

V

V

I

L

A

H

H

D

R

I

L

N

S

Q

-

A

T

C

V

R

R

Y

N

A

A

H

D

H

V

L

I

A

A

V

G

M

F

H

D

G

G

K

V

L

I

V

V

A

E

D

Q

G

G

A

N

P

H

G

D

Q

-

V

-

I

-

T

-

A

-

E

E

L

L

M

M

R

R

Q

E

Sites not aligning to the query:

binding N-(2,4-di-tert-butyl-5-hydroxyphenyl)-4-oxo-1,4-dihydroquinoline-3-carboxamide: 307, 311, 779, 780

1jj7A Crystal structure of thE C-terminal atpase domain of human tap1 (see paper)
32% identity, 80% coverage: 18:227/263 of query aligns to 29:239/251 of 1jj7A

query
sites
1jj7A

I
x
V

V

L

Q

Q

G

G

L

L

S

T

F

F

V

T

P

L

P

R

T

P

G

G

K

E

V

V

T

T

A

A

L

L

I

V

G

G

P

P

N
|
N

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

V

L

A

K

A

A

L

F

L

A

Q

R

N

I

L

L

Y

K

Q

P

P

T

T

Q

G

G

G

E

Q

L

L

T

L

L

L

D

D

G

G

H

K

A

P

Y

L

T

P

S

Q

L

Y

S

E

A

H

R

R

Q

Y

L

L

A

H

R

R

Q

Q

I

V

A

A

F

A

L

V

-

G

-

Q

-

E

P

P

Q

Q

V

V

L

F

P

-

V

-

P

-

E

-

G

G

V

R

S

S

V

L

R

Q

Q

E

L

N

V

I

A

A

Y

Y

G

G

-

L

-

T

R

Q

S

K

P

P

H

T

N

M

S

E

L

E

W

I

G

T

R

A

L

A

S

A

G

V

N

K

D

S

Q

G

A

A

H

H

V

S

T

F

Q

I

A

S

M

G

Q

L

R

P

L

Q

E

G

L

Y

D

D

T

T

L

E

A

V

D

D

R

E

A

A

L

G

A

S

D

Q

L

L

S

S

G

G

Q

Q

R

R

Q

Q

R

A

A

V

W

A

L

L

A

A

M

R

V

A

L

L

A

I

Q

R

N

K

A

P

P

C

V

V

V

L

L

I

L

L

D

D

E

D

P

A

T

T

T

S

Y

A

L

L

D

D

I

A

S

N

H

S

Q

Q

V

L

E

Q

L

V

L

E

D

Q

L

L

M

L

G

Y

E

E

L

S

A

P

G

E

E

R

G

Y

K

S

T

R

V

S

I

V

T

L

V

L

L

I

H

T

D

Q

I

H

N

L

Q

S

A

L

C

V

R

E

Y

Q

A

A

H

D

H

H

L

I

A

L

V

F

M

L

H

E

G

G

K

A

L

I

V

R

A

E

D

G

G

G

A

T

P

H

G

Q

Q

Q

V

L

I

M

binding adenosine-5'-diphosphate: V29 (≠ I18), N49 (= N38), G50 (= G39), S51 (≠ C40), G52 (= G41), K53 (= K42), S54 (= S43), T55 (= T44)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 21

4xwkA P-glycoprotein co-crystallized with bde-100 (see paper)
30% identity, 83% coverage: 17:234/263 of query aligns to 375:585/1182 of 4xwkA

query
sites
4xwkA

R

Q

I

I

V

L

Q

K

G

G

L

L

S

N

F

L

V

K

P

V

P

K

T

S

G

G

K

Q

V

T

T

V

A

A

L

L

I

V

G

G

P

N

N

S

G

G

C

C

G

G

K

K

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S

T

T

L

T

L

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A

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R

I

L

L

Y

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D

P

P

T

L

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D

G

G

E

M

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L

I

D

D

G

G

H

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A

D

Y

I

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S

T

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I

S

N

A

V

R

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Y

L

L

A

R

R

E

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I

I

I

A

G

F

V

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S

Q

Q

V

E

L

-

P

P

V

V

P

L

E

F

G

A

V

T

S

T

V

I

R

A

Q

E

L

N

V

I

A

R

Y

Y

G

G

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R

S

E

P

D

H

V

N

-

S

T

L

M

W

D

G

E

R

I

L

E

S

K

G

A

N

V

D

K

Q

E

A

A

H

N

V

A

T

Y

Q

D

A

F

M

I

Q

M

R

K

L

L

-

P

-

H

E

Q

L

F

D

D

T

T

L

L

A

V

D

G

R

E

A

R

L

G

A

A

D

Q

L

L

S

S

G

G

Q

Q

R

K

Q

Q

R

R

A

I

W

A

L

I

A

A

M

R

V

A

L

L

A

V

Q

R

N

N

A

P

P

K

V

I

V

L

L

L

D

D

E

E

P

A

T

T

T

S

Y

A

L

L

D

D

I

T

S

E

H

S

Q

E

V

A

E

V

L

V

L

Q

D

A

L

A

M

L

G

D

E

K

L

-

A

A

G

R

E

E

G

G

K

R

T

T

V

T

I

I

T

V

V

I

L

A

H

H

D

R

I

L

N

S

Q

-

A

T

C

V

R

R

Y

N

A

A

H

D

H

V

L

I

A

A

V

G

M

F

H

D

G

G

K

V

L

I

V

V

A

E

D

Q

G

G

A

N

P

H

G

D

Q

-

V

-

I

-

T

-

A

-

E

E

L

L

M

M

R

R

Q

E

Sites not aligning to the query:

binding 2,4-dibromophenyl 2,4,6-tribromophenyl ether: 274, 281, 302, 633, 637, 664

7zdaC If(apo/asym) conformation of cyddc in adp+pi(cydc)/atp(cydd) bound state (dataset-2) (see paper)
29% identity, 79% coverage: 19:227/263 of query aligns to 356:560/572 of 7zdaC

query
sites
7zdaC

V

L

Q

K

G

G

L

I

S

S

F

L

V

Q

P

V

P

N

T

A

G

G

K

E

V

H

T

I

A

A

L

I

I

L

G

G

P

R

N

T

G
|
G

C

C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

K

Q

A

Q

F

L

A

T

R

R

I

A

L

W

K

D

P

P

T

Q

Q

Q

G

G

E

E

L

I

T

L

L

L

D

N

G

D

H

S

A

P

Y

I

T

A

S

S

L

L

S

N

A

E

R

A

Q

A

L

L

A

R

R

Q

Q

T

I

I

A

S

F

V

L

V

P

P

Q
|
Q

V

R

L

V

P

H

V

L

P

-

E

-

G

-

V

-

S

-

V

-

R

-

Q

-

L

-

V

-

A

-

Y

F

G

S

R

A

S

T

P

L

H

R

N

D

S

N

L

L

W

L

G

L

R

A

L

S

S

P

G

G

N

S

D

S

Q

D

A

E

H

A

V

L

T

S

Q

E

A

I

M

L

Q

R

R

R

L

V

E

G

L

L

D

E

T

K

L

L

A

L

D

E

R

D

A

A

L

G

A

L

D

N

-

S

-

W

-

L

-

G

-

E

-

G

-

R

-

Q

L

L

S

S

G

G

Q

E

R

L

Q

R

R

R

A

L

W

A

L

I

A

A

M

R

V

A

L

L

A

L

Q

H

N

D

A

A

P

P

V

L

V

V

L

L

D

D

E

E

P

P

T

T

T

E

Y

G

L

L

D

D

I

A

S

T

H

T

Q

E

V

S

E

Q

L

I

L

L

D

E

L

L

M

L

G

A

E

E

L

M

A

M

G

R

E

E

G

-

K

K

T

T

V

V

I

L

T

M

V

V

L

T

H

H

D

R

I

L

N

R

Q

G

A

L

C

S

R

R

Y

F

A

-

H

Q

L

I

A

I

V

V

M

M

H

D

G

N

G

G

K

Q

L

I

V

I

A

E

D

Q

G

G

A

T

P

H

G

A

Q

E

V

L

I

L

binding adenosine-5'-diphosphate: G376 (= G39), G378 (= G41), K379 (= K42), S380 (= S43), T381 (= T44)
binding magnesium ion: S380 (= S43), Q421 (= Q84)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 112, 348

Query Sequence

>GFF3532 FitnessBrowser__WCS417:GFF3532
MSILQARELDIGYGTTRIVQGLSFVPPTGKVTALIGPNGCGKSTLLKAFARILKPTQGEL
TLDGHAYTSLSARQLARQIAFLPQVLPVPEGVSVRQLVAYGRSPHNSLWGRLSGNDQAHV
TQAMQRLELDTLADRALADLSGGQRQRAWLAMVLAQNAPVVLLDEPTTYLDISHQVELLD
LMGELAGEGKTVITVLHDINQACRYAHHLAVMHGGKLVADGAPGQVITAELMRQVFEVQV
QVMSEPVAGTPMCLIKKSTRPHT

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory