SitesBLAST
Comparing GFF3617 FitnessBrowser__Phaeo:GFF3617 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
8b7sA Crystal structure of the chloramphenicol-inactivating oxidoreductase from novosphingobium sp (see paper)
33% identity, 96% coverage: 20:548/552 of query aligns to 5:450/458 of 8b7sA
- binding flavin-adenine dinucleotide: G11 (= G26), G13 (= G28), S14 (= S29), A15 (= A30), E35 (= E50), A36 (= A51), W47 (= W77), P65 (= P95), G67 (= G97), V180 (= V234), A214 (= A268), G215 (= G269), A218 (= A272), T270 (≠ L350), Y391 (≠ F488), A424 (= A522), I435 (≠ T533), N436 (= N534)
5nccA Structure of fatty acid photodecarboxylase in complex with fad and palmitic acid (see paper)
33% identity, 96% coverage: 20:548/552 of query aligns to 24:569/578 of 5nccA
- active site: R347 (vs. gap), L420 (≠ F402), I421 (≠ Q403), S507 (≠ V487), A509 (≠ H489), G552 (= G531), Q553 (≠ N532)
- binding flavin-adenine dinucleotide: G30 (= G26), G32 (= G28), T33 (≠ S29), A34 (= A30), L53 (= L49), E54 (= E50), A55 (= A51), F74 (= F70), W80 (= W77), A98 (≠ P95), G100 (= G97), G105 (= G102), S106 (= S103), N110 (= N107), A111 (= A108), T112 (≠ L109), L113 (≠ V110), V238 (= V234), A278 (= A268), H282 (≠ A272), L286 (= L276), N508 (≠ F488), Q553 (≠ N532), T554 (= T533), G555 (≠ N534), V558 (≠ T537)
6yrvAAA structure of fap after illumination at 100k (see paper)
33% identity, 96% coverage: 20:548/552 of query aligns to 8:560/573 of 6yrvAAA
- binding carbon dioxide: R375 (≠ Y373), N499 (≠ F488)
- binding flavin-adenine dinucleotide: G14 (= G26), G16 (= G28), T17 (≠ S29), A18 (= A30), L37 (= L49), E38 (= E50), A39 (= A51), F58 (= F70), W64 (= W77), A82 (≠ P95), G89 (= G102), S90 (= S103), N94 (= N107), A95 (= A108), T96 (≠ L109), L97 (≠ V110), M191 (≠ N203), V222 (= V234), C264 (≠ S267), A265 (= A268), G266 (= G269), H269 (≠ A272), N499 (≠ F488), A534 (= A522), Q544 (≠ N532), T545 (= T533), G546 (≠ N534)
- binding heptadecane: V377 (≠ N375), G379 (≠ T378), M380 (≠ Y379), G386 (= G385), T389 (≠ E388), Y390 (≠ V389), F393 (≠ P392), T408 (≠ I399), Q410 (≠ G401)
A0A248QE08 Fatty acid photodecarboxylase, chloroplastic; CvFAP; EC 4.1.1.106 from Chlorella variabilis (Green alga) (see paper)
33% identity, 96% coverage: 20:548/552 of query aligns to 84:636/654 of A0A248QE08
4ha6A Crystal structure of pyridoxine 4-oxidase - pyridoxamine complex (see paper)
34% identity, 94% coverage: 21:541/552 of query aligns to 3:497/508 of 4ha6A
- active site: F360 (= F402), G361 (≠ Q403), H444 (≠ V487), H446 (= H489), G487 (= G531), P488 (≠ N532)
- binding flavin-adenine dinucleotide: G8 (= G26), G10 (= G28), S11 (= S29), A12 (= A30), E32 (= E50), A33 (= A51), W58 (= W77), R77 (= R96), G78 (= G97), G83 (= G102), S84 (= S103), L87 (≠ I106), H88 (≠ N107), A89 (= A108), M90 (≠ L109), G91 (≠ V110), V218 (= V234), A251 (= A268), G252 (= G269), E255 (≠ A272), H445 (≠ F488), A478 (= A522), P488 (≠ N532), I489 (≠ T533), H490 (≠ N534)
- binding 4-(aminomethyl)-5-(hydroxymethyl)-2-methylpyridin-3-ol: A89 (= A108), S314 (≠ N353), H444 (≠ V487), H446 (= H489)
3t37A Crystal structure of pyridoxine 4-oxidase from mesorbium loti
34% identity, 94% coverage: 21:541/552 of query aligns to 3:497/509 of 3t37A
- active site: F360 (= F402), G361 (≠ Q403), H444 (≠ V487), H446 (= H489), G487 (= G531), P488 (≠ N532)
- binding flavin-adenine dinucleotide: G8 (= G26), G10 (= G28), S11 (= S29), A12 (= A30), E32 (= E50), A33 (= A51), W58 (= W77), R77 (= R96), G78 (= G97), R79 (≠ K98), G83 (= G102), S84 (= S103), H88 (≠ N107), A89 (= A108), G91 (≠ V110), R217 (≠ L233), V218 (= V234), A251 (= A268), E255 (≠ A272), H445 (≠ F488), A478 (= A522), P488 (≠ N532), I489 (≠ T533), H490 (≠ N534)
E4QP00 5-(hydroxymethyl)furfural oxidase; 5-hydroxymethylfurfural oxidase; HMFO; Thiol oxidase; EC 1.1.3.47; EC 1.8.3.- from Methylovorus sp. (strain MP688) (see paper)
31% identity, 96% coverage: 18:549/552 of query aligns to 4:528/531 of E4QP00
- V101 (≠ I106) mutation to H: Abolishes activity.
- M103 (≠ A108) mutation to A: 16-fold reduction in catalytic efficiency on vanillyl alcohol.
- V367 (vs. gap) mutation to K: 1.6-fold reduction in catalytic efficiency on vanillyl alcohol. Shows significantly improved activity on the aldehyde 5-formyl-2-furancarboxylate, which results in a better 5-hydroxymethylfurfural to 2,5-furandicarboxylate conversion.; mutation to R: 1.4-fold reduction in catalytic efficiency on vanillyl alcohol. Shows significantly improved activity on the aldehyde 5-formyl-2-furancarboxylate, which results in a better 5-hydroxymethylfurfural to 2,5-furandicarboxylate conversion. Displays a catalytic efficiency toward 5-formyl-2-furancarboxylate that is over 1000-fold higher than that for wild-type; when associated with F-466.
- W369 (≠ Q403) mutation to A: 7.5-fold reduction in catalytic efficiency on vanillyl alcohol.
- V465 (= V487) mutation to A: 18-fold reduction in catalytic efficiency on vanillyl alcohol.
- W466 (≠ F488) mutation to A: 39-fold reduction in catalytic efficiency on vanillyl alcohol. In contrast to wild-type, is active on secondary alcohols, such as (S)-1-phenylethanol, and is strictly enantionselective as this mutant has no activity on (R)-1-phenylethanol. Shows increased activity on the aldehyde 5-formyl-2-furancarboxylate.; mutation to F: 3.4-fold reduction in catalytic efficiency on vanillyl alcohol. In contrast to wild-type, is active on secondary alcohols, such as (S)-1-phenylethanol, and is strictly enantionselective as this mutant has no activity on (R)-1-phenylethanol. Shows increased activity on the aldehyde 5-formyl-2-furancarboxylate. Displays a catalytic efficiency toward 5-formyl-2-furancarboxylate that is over 1000-fold higher than that for wild-type; when associated with R-367.
- H467 (= H489) mutation to A: Abolishes activity.
- N511 (= N532) mutation to A: 53-fold reduction in catalytic efficiency on vanillyl alcohol.
4udqA Crystal structure of 5-hydroxymethylfurfural oxidase (hmfo) in the reduced state
31% identity, 96% coverage: 20:549/552 of query aligns to 2:524/525 of 4udqA
- active site: L331 (≠ C355), F364 (= F402), W365 (≠ Q403), V461 (= V487), H463 (= H489), A506 (≠ G531), N507 (= N532)
- binding flavin-adenine dinucleotide: G8 (= G26), G10 (= G28), T11 (≠ S29), A12 (= A30), E32 (= E50), A33 (= A51), W64 (≠ D72), G88 (= G97), G93 (= G102), G94 (≠ S103), N98 (= N107), M99 (≠ A108), V101 (= V110), V229 (= V234), T261 (≠ S267), A262 (= A268), W462 (≠ F488), H463 (= H489), A497 (= A522), N507 (= N532), T508 (= T533), N509 (= N534), T512 (= T537)
5oc1A Crystal structure of aryl-alcohol oxidase from pleurotus eryngii in complex with p-anisic acid (see paper)
29% identity, 96% coverage: 20:548/552 of query aligns to 2:561/565 of 5oc1A
- active site: V339 (≠ R335), N413 (≠ F402), A414 (≠ Q403), I499 (≠ V487), H501 (= H489), A544 (≠ G531), H545 (≠ N532)
- binding 4-methoxybenzoic acid: Y91 (≠ A108), I356 (≠ V352), I390 (≠ T382), F396 (vs. gap), T412 (≠ G401), I499 (≠ V487), H501 (= H489), H545 (≠ N532)
- binding flavin-adenine dinucleotide: G8 (= G26), G10 (= G28), N11 (≠ S29), A12 (= A30), E32 (= E50), A33 (= A51), W60 (= W77), P78 (= P95), G80 (= G97), G85 (= G102), S86 (= S103), H90 (≠ N107), Y91 (≠ A108), V93 (= V110), V230 (= V234), S270 (= S267), A271 (= A268), G272 (= G269), F500 (= F488), H545 (≠ N532), T546 (= T533), Q547 (≠ N534), I550 (≠ T537)
3fimB Crystal structure of aryl-alcohol-oxidase from pleurotus eryingii (see paper)
29% identity, 96% coverage: 20:548/552 of query aligns to 2:561/565 of 3fimB
- active site: V339 (≠ R335), N413 (≠ F402), A414 (≠ Q403), I499 (≠ V487), H501 (= H489), A544 (≠ G531), H545 (≠ N532)
- binding flavin-adenine dinucleotide: G8 (= G26), N11 (≠ S29), A12 (= A30), E32 (= E50), A33 (= A51), W60 (= W77), P78 (= P95), G80 (= G97), G85 (= G102), S86 (= S103), H90 (≠ N107), Y91 (≠ A108), V93 (= V110), V230 (= V234), S270 (= S267), A271 (= A268), F500 (= F488), H501 (= H489), H545 (≠ N532), T546 (= T533), Q547 (≠ N534), I550 (≠ T537)
8bxlB Patulin synthase from penicillium expansum
30% identity, 95% coverage: 20:545/552 of query aligns to 14:583/590 of 8bxlB
- binding flavin-adenine dinucleotide: G20 (= G26), G22 (= G28), T23 (≠ S29), A24 (= A30), E44 (= E50), A45 (= A51), W80 (= W77), G100 (= G97), G105 (= G102), S106 (= S103), R109 (≠ I106), N110 (= N107), Y111 (≠ A108), A113 (≠ V110), L253 (= L233), A254 (≠ V234), A288 (= A268), Q292 (≠ A272), F525 (= F488), D559 (= D521), A560 (= A522), H570 (≠ N532), P571 (≠ T533), Q572 (≠ N534), L575 (≠ T537)
4yntA Crystal structure of aspergillus flavus fad glucose dehydrogenase (see paper)
29% identity, 97% coverage: 16:550/552 of query aligns to 1:565/570 of 4yntA
- active site: V342 (= V326), F413 (= F402), W414 (≠ Q403), N502 (≠ V487), H504 (= H489), G546 (= G531), H547 (≠ N532)
- binding dihydroflavine-adenine dinucleotide: G13 (= G28), T14 (≠ S29), S15 (≠ A30), E35 (= E50), A36 (= A51), F56 (= F70), W62 (= W77), R80 (≠ P95), G82 (= G97), G87 (= G102), T88 (≠ S103), N92 (= N107), G93 (≠ A108), M94 (≠ L109), A95 (≠ V110), A234 (≠ V234), A274 (= A268), R278 (≠ A272), F503 (= F488), A537 (= A522), H547 (≠ N532), L548 (≠ T533), V549 (≠ N534), L552 (≠ T537)
7vzsA Fad-dpendent glucose dehydrogenase complexed with an inhibitor at ph7.56
29% identity, 97% coverage: 18:550/552 of query aligns to 2:564/566 of 7vzsA
- binding D-glucal: Y6 (= Y22), L22 (= L38), N25 (≠ S41), Y51 (= Y66), I349 (= I334), Q356 (≠ A341), E411 (≠ G401), E444 (= E434), W445 (≠ E435), K448 (≠ S438), R499 (≠ G485), N501 (≠ V487), H546 (≠ N532), K563 (≠ L549)
- binding flavin-adenine dinucleotide: G10 (= G26), G12 (= G28), T13 (≠ S29), S14 (≠ A30), E34 (= E50), A35 (= A51), Y51 (= Y66), F55 (= F70), W61 (= W77), R79 (≠ P95), G81 (= G97), G86 (= G102), T87 (≠ S103), N91 (= N107), G92 (≠ A108), M93 (≠ L109), A94 (≠ V110), T232 (≠ L233), A233 (≠ V234), A273 (= A268), G274 (= G269), R277 (≠ A272), F502 (= F488), A536 (= A522), H546 (≠ N532), L547 (≠ T533), V548 (≠ N534), L551 (≠ T537)
Sites not aligning to the query:
4ynuA Crystal structure of aspergillus flavus fadgdh in complex with d- glucono-1,5-lactone (see paper)
29% identity, 97% coverage: 18:550/552 of query aligns to 2:564/569 of 4ynuA
- active site: V341 (= V326), F412 (= F402), W413 (≠ Q403), N501 (≠ V487), H503 (= H489), G545 (= G531), H546 (≠ N532)
- binding flavin-adenine dinucleotide: G12 (= G28), T13 (≠ S29), S14 (≠ A30), E34 (= E50), A35 (= A51), Y51 (= Y66), F55 (= F70), W61 (= W77), R79 (≠ P95), G81 (= G97), G86 (= G102), T87 (≠ S103), N91 (= N107), G92 (≠ A108), T232 (≠ L233), A233 (≠ V234), A273 (= A268), G274 (= G269), R277 (≠ A272), F502 (= F488), A536 (= A522), H546 (≠ N532), L547 (≠ T533), V548 (≠ N534), L551 (≠ T537)
- binding D-glucono-1,5-lactone: Y51 (= Y66), E411 (≠ G401), A496 (≠ E482), N497 (≠ R483), R499 (≠ G485), R499 (≠ G485), N501 (≠ V487), H503 (= H489), H546 (≠ N532)
2jbvA Crystal structure of choline oxidase reveals insights into the catalytic mechanism (see paper)
30% identity, 96% coverage: 20:548/552 of query aligns to 14:526/527 of 2jbvA
- active site: I333 (≠ C355), P377 (≠ F402), N378 (≠ Q403), V464 (= V487), H466 (= H489), V509 (≠ G531), N510 (= N532)
- binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl (2R,3S,4S)-5-[(4aS,10aR)-7,8-dimethyl-2,4-dioxo-1,3,4,4a,5,10a-hexahydrobenzo[g]pteridin-10(2H)-yl]-2,3,4-trihydroxypentyl dihydrogen diphosphate: G22 (= G28), S23 (= S29), E44 (= E50), A45 (= A51), W71 (= W77), A90 (≠ G97), G95 (= G102), C96 (≠ S103), H99 (≠ I106), N100 (= N107), S101 (≠ A108), I103 (≠ V110), R231 (≠ L233), A232 (≠ V234), T269 (≠ A268), G270 (= G269), D273 (≠ A272), V464 (= V487), Y465 (≠ F488), H466 (= H489), D499 (= D521), A500 (= A522), N510 (= N532), P511 (≠ T533), N512 (= N534), V515 (≠ T537)
4mjwA Crystal structure of choline oxidase in complex with the reaction product glycine betaine (see paper)
30% identity, 96% coverage: 20:551/552 of query aligns to 14:529/532 of 4mjwA
- active site: I333 (≠ C355), P377 (≠ F402), N378 (≠ Q403), V464 (= V487), H466 (= H489), V509 (≠ G531), N510 (= N532)
- binding flavin-adenine dinucleotide: G20 (= G26), G22 (= G28), S23 (= S29), E44 (= E50), A45 (= A51), W71 (= W77), R89 (= R96), A90 (≠ G97), G95 (= G102), C96 (≠ S103), H99 (≠ I106), N100 (= N107), S101 (≠ A108), I103 (≠ V110), R231 (≠ L233), A232 (≠ V234), T269 (≠ A268), G270 (= G269), D273 (≠ A272), Y465 (≠ F488), H466 (= H489), A500 (= A522), N510 (= N532), P511 (≠ T533), N512 (= N534), V515 (≠ T537)
3ljpA Crystal structure of choline oxidase v464a mutant (see paper)
30% identity, 96% coverage: 20:551/552 of query aligns to 14:529/530 of 3ljpA
- active site: I333 (≠ C355), P377 (≠ F402), N378 (≠ Q403), A464 (≠ V487), H466 (= H489), V509 (≠ G531), N510 (= N532)
- binding dihydroflavine-adenine dinucleotide: G22 (= G28), S23 (= S29), E44 (= E50), A45 (= A51), W71 (= W77), R89 (= R96), A90 (≠ G97), G95 (= G102), C96 (≠ S103), H99 (≠ I106), N100 (= N107), S101 (≠ A108), I103 (≠ V110), A232 (≠ V234), T269 (≠ A268), D273 (≠ A272), Y465 (≠ F488), H466 (= H489), D499 (= D521), A500 (= A522), N510 (= N532), P511 (≠ T533), N512 (= N534), V515 (≠ T537)
4h7uA Crystal structure of pyranose dehydrogenase from agaricus meleagris, wildtype (see paper)
28% identity, 96% coverage: 20:551/552 of query aligns to 16:575/577 of 4h7uA
- active site: A343 (= A336), V426 (vs. gap), Y510 (≠ V487), H512 (= H489), A555 (≠ G531), H556 (≠ N532)
- binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl (2R,3S,4S)-2,3,4-trihydroxy-5-[(4aR)-4a-hydroxy-7,8-dimethyl-2,4-dioxo-3,4,4a,5-tetrahydrobenzo[g]pteridin-10(2H)-yl]pentyl dihydrogen diphosphate (non-preferred name): G22 (= G26), G24 (= G28), T25 (≠ S29), A26 (= A30), E46 (= E50), A47 (= A51), W74 (= W77), G99 (= G102), C100 (≠ S103), H103 (≠ I106), N104 (= N107), G105 (≠ A108), V107 (= V110), L242 (= L233), V243 (= V234), G282 (≠ A268), G283 (= G269), A286 (= A272), H512 (= H489), A546 (= A522), H556 (≠ N532), T557 (= T533), Q558 (≠ N534), V561 (≠ T537)
Q3L245 Pyranose dehydrogenase 1; PDH 1; Pyranose:quinone oxidoreductase 1; EC 1.1.99.29 from Leucoagaricus meleagris (Western flat-topped agaric) (Agaricus meleagris) (see 2 papers)
28% identity, 96% coverage: 20:551/552 of query aligns to 41:600/602 of Q3L245
- N100 (≠ K78) modified: carbohydrate, N-linked (GlcNAc...) asparagine
- H128 (≠ I106) modified: Tele-8alpha-FAD histidine
- N344 (= N305) modified: carbohydrate, N-linked (GlcNAc...) asparagine
- H537 (= H489) active site, Proton acceptor
- H581 (≠ N532) active site
Sites not aligning to the query:
3q9tA Crystal structure analysis of formate oxidase (see paper)
27% identity, 96% coverage: 20:548/552 of query aligns to 7:569/577 of 3q9tA
- active site: A335 (= A336), D422 (vs. gap), A508 (≠ V487), H510 (= H489), C552 (≠ G531), R553 (≠ N532)
- binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl(2R,3S,4S)-5-(8-formyl-7-methyl-2,4-dioxo-3,4-dihydrobenzo[g]pteridin-10(2H)-yl)-2,3,4-trihydroxypentyl dihydrogendiphosphate: G15 (= G28), T16 (≠ S29), E37 (= E50), A38 (= A51), W65 (= W77), T85 (≠ P95), R86 (= R96), G87 (= G97), G92 (= G102), S93 (= S103), N97 (= N107), Y98 (≠ A108), F99 (≠ L109), T100 (≠ V110), S229 (≠ V234), S265 (= S267), Q266 (≠ A268), E270 (≠ A272), F509 (= F488), D542 (= D521), A543 (= A522), R553 (≠ N532), I554 (≠ T533), Q555 (≠ N534), V558 (≠ T537)
Query Sequence
>GFF3617 FitnessBrowser__Phaeo:GFF3617
MLCNDLTTGDMRAVRQVTGFDYIIIGAGSAGCVLADRLSRSGRHKVLILEAGGRGRSPWI
ALPLGYGKTFFDPAVNWKYESVREEALAGRAGYWPRGKGVGGSGAINALVYARGLPQDFD
DWEAAGAIGWGWTSVRQGYEAMETQVAPDGQRRGSGPLHVQDVSDQIHPANRHFFAAAAE
LGLPRTDDINDPGGEGATAYRINTSGGRRMHSARAFLTPALKRKNVTLMTDALVERITFE
GRRAVSVQVRRRGKSMSIAAGREIVLSAGAVASPGLLQKSGIGPAELLRRHGIAIVDDQP
QVGGNLQDHLGINYYFRATEPTLNNVLSPLTGKIRAALHYALWRRGPLALSVNQCGGYFR
SAQSLTRPDQQLYFNPVTYTTTPEGTREVIRPDPFAGFILGFQPSRPTSRGRIDICSADV
SAAPLIRPNSLATEEDRSQVIAGGRLCQRLATTPALDGLIEVAMGTDLRQMADADILSDF
RERCGTVFHPVGTCRMGTDASTSVVCPKLKLHGLDGVRVVDASVFPNITSGNTNAPTMML
AHRAADLILEAT
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory