SitesBLAST
Comparing GFF364 FitnessBrowser__psRCH2:GFF364 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3rfvA Crystal structure of uronate dehydrogenase from agrobacterium tumefaciens complexed with nadh and product (see paper)
52% identity, 94% coverage: 11:265/271 of query aligns to 4:258/265 of 3rfvA
- binding D-galactaro-1,5-lactone: S74 (= S82), S110 (= S118), N111 (= N119), H112 (= H120), Y135 (= Y143), S164 (= S172), R173 (= R181), F257 (= F264)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G9 (= G16), G12 (= G19), Q13 (≠ G20), L14 (= L21), D33 (= D40), L34 (≠ I41), D50 (= D58), L51 (= L59), L70 (≠ F78), G71 (= G79), G72 (= G80), S74 (= S82), A108 (= A116), S110 (= S118), Y135 (= Y143), K139 (= K147), I162 (= I170), S164 (= S172), C165 (≠ S173)
Q7CRQ0 Uronate dehydrogenase; D-galacturonate dehydrogenase; D-glucuronate dehydrogenase; Hexuronate dehydrogenase; EC 1.1.1.203 from Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens (strain C58)) (see paper)
52% identity, 94% coverage: 11:265/271 of query aligns to 3:257/265 of Q7CRQ0
- Y134 (= Y143) mutation to A: 0.1% of wild-type activity.
3rfxA Crystal structure of uronate dehydrogenase from agrobacterium tumefaciens, y136a mutant complexed with NAD (see paper)
52% identity, 94% coverage: 11:265/271 of query aligns to 4:258/265 of 3rfxA
4id9A Crystal structure of a short-chain dehydrogenase/reductase superfamily protein from agrobacterium tumefaciens (target efi-506441) with bound NAD, monoclinic form 1
31% identity, 58% coverage: 12:169/271 of query aligns to 2:158/321 of 4id9A
- active site: S106 (= S118), Y132 (= Y143), K136 (= K147)
- binding nicotinamide-adenine-dinucleotide: G9 (= G19), R10 (≠ G20), V11 (≠ L21), D30 (= D40), L31 (≠ I41), R32 (≠ A42), S44 (≠ D58), L45 (= L59), L64 (≠ F78), A66 (≠ G80), V81 (≠ A93), S106 (= S118), Y132 (= Y143), K136 (= K147)
Sites not aligning to the query:
4id9B Crystal structure of a short-chain dehydrogenase/reductase superfamily protein from agrobacterium tumefaciens (target efi-506441) with bound NAD, monoclinic form 1
31% identity, 58% coverage: 12:169/271 of query aligns to 3:159/328 of 4id9B
- active site: S107 (= S118), Y133 (= Y143), K137 (= K147)
- binding nicotinamide-adenine-dinucleotide: G10 (= G19), R11 (≠ G20), V12 (≠ L21), D31 (= D40), L32 (≠ I41), S45 (≠ D58), L46 (= L59), L65 (≠ F78), A67 (≠ G80), V82 (≠ A93), Y133 (= Y143), K137 (= K147)
Sites not aligning to the query:
3aw9A Structure of udp-galactose 4-epimerase mutant
30% identity, 59% coverage: 11:169/271 of query aligns to 2:157/304 of 3aw9A
- active site: A105 (= A116), S107 (= S118), S108 (≠ N119), T109 (≠ H120), Y131 (= Y143), K135 (= K147)
- binding galactose-uridine-5'-diphosphate: P69 (≠ S82), V71 (≠ E84), S107 (= S118), Y131 (= Y143)
- binding nicotinamide-adenine-dinucleotide: G7 (= G16), G10 (= G19), F11 (≠ G20), I12 (≠ L21), D31 (= D40), I32 (= I41), R46 (≠ C57), D47 (= D58), L48 (= L59), F65 (= F78), A66 (≠ G79), A67 (≠ G80), E82 (≠ A93), A105 (= A116), S106 (= S117), Y131 (= Y143), K135 (= K147)
Sites not aligning to the query:
- active site: 166, 169
- binding galactose-uridine-5'-diphosphate: 160, 168, 170, 173, 188, 193, 195, 197, 234, 263, 266
- binding nicotinamide-adenine-dinucleotide: 158, 160, 161, 168
6jygD Crystal structure of l-threonine dehydrogenase from phytophthora infestans (see paper)
31% identity, 59% coverage: 11:169/271 of query aligns to 2:162/307 of 6jygD
- binding citrate anion: Q11 (≠ G20), L73 (≠ S82), S74 (≠ V83), Y136 (= Y143)
- binding nicotinamide-adenine-dinucleotide: G7 (= G16), T9 (≠ A18), G10 (= G19), Q11 (≠ G20), I12 (≠ L21), D33 (≠ M44), I34 (≠ A45), K35 (≠ P46), D47 (= D58), V48 (≠ L59), M69 (≠ F78), A70 (≠ G79), S71 (≠ G80), L73 (≠ S82), V87 (≠ A93), P109 (≠ A116), S110 (= S117), T111 (≠ S118), Y136 (= Y143), K140 (= K147)
Sites not aligning to the query:
3a4vA Crystal structure of pyruvate bound l-threonine dehydrogenase from hyperthermophilic archaeon thermoplasma volcanium (see paper)
25% identity, 58% coverage: 12:169/271 of query aligns to 2:163/311 of 3a4vA
- active site: T112 (≠ S118), Y137 (= Y143), K141 (= K147)
- binding nicotinamide-adenine-dinucleotide: S8 (≠ A18), G9 (= G19), Q10 (≠ G20), I11 (≠ L21), D32 (≠ I41), I33 (≠ A42), L46 (≠ C57), D47 (= D58), V48 (≠ L59), L69 (≠ F78), A70 (≠ G79), G71 (= G80), L73 (≠ S82), P110 (≠ A116), S111 (= S117), T112 (≠ S118), Y137 (= Y143), K141 (= K147)
- binding pyruvic acid: S74 (≠ V83), Y137 (= Y143)
Sites not aligning to the query:
3a1nA Crystal structure of l-threonine dehydrogenase from hyperthermophilic archaeon thermoplasma volcanium (see paper)
25% identity, 58% coverage: 12:169/271 of query aligns to 2:163/315 of 3a1nA
- active site: T112 (≠ S118), Y137 (= Y143), K141 (= K147)
- binding nicotinamide-adenine-dinucleotide: S8 (≠ A18), G9 (= G19), Q10 (≠ G20), I11 (≠ L21), D32 (≠ I41), I33 (≠ A42), L46 (≠ C57), D47 (= D58), L69 (≠ F78), A70 (≠ G79), G71 (= G80), L73 (≠ S82), S111 (= S117), Y137 (= Y143), K141 (= K147)
Sites not aligning to the query:
7cgvA Full consensus l-threonine 3-dehydrogenase, fctdh-iiym (NAD+ bound form) (see paper)
29% identity, 59% coverage: 11:169/271 of query aligns to 2:165/310 of 7cgvA
- binding nicotinamide-adenine-dinucleotide: G7 (= G16), G10 (= G19), Q11 (≠ G20), I12 (≠ L21), D33 (= D40), I34 (= I41), R35 (vs. gap), L48 (≠ V54), D49 (= D58), V50 (≠ L59), A72 (≠ G79), A73 (≠ G80), L75 (vs. gap), P112 (≠ A116), S114 (= S118), Y139 (= Y143), K143 (= K147)
Sites not aligning to the query:
3a9wA Crystal structure of l-threonine bound l-threonine dehydrogenase (y137f) from hyperthermophilic archaeon thermoplasma volcanium (see paper)
24% identity, 58% coverage: 12:169/271 of query aligns to 2:163/315 of 3a9wA
- binding nicotinamide-adenine-dinucleotide: S8 (≠ A18), G9 (= G19), Q10 (≠ G20), I11 (≠ L21), D32 (≠ I41), I33 (≠ A42), L46 (≠ C57), D47 (= D58), V48 (≠ L59), L69 (≠ F78), A70 (≠ G79), L73 (≠ S82), V87 (≠ A93), P110 (≠ A116), S111 (= S117), K141 (= K147)
- binding threonine: S74 (≠ V83), T112 (≠ S118), F137 (≠ Y143)
Sites not aligning to the query:
3ajrA Crystal structure of l-3-hydroxynorvaline bound l-threonine dehydrogenase (y137f) from hyperthermophilic archaeon thermoplasma volcanium (see paper)
24% identity, 58% coverage: 12:169/271 of query aligns to 2:163/314 of 3ajrA
- binding nicotinamide-adenine-dinucleotide: S8 (≠ A18), G9 (= G19), Q10 (≠ G20), I11 (≠ L21), D32 (≠ I41), I33 (≠ A42), L46 (≠ C57), D47 (= D58), V48 (≠ L59), L69 (≠ F78), A70 (≠ G79), G71 (= G80), L73 (≠ S82), P110 (≠ A116), S111 (= S117), T112 (≠ S118), F137 (≠ Y143), K141 (= K147)
- binding (3R)-3-hydroxy-L-norvaline: S74 (≠ V83), T112 (≠ S118), I113 (≠ N119), F137 (≠ Y143)
Sites not aligning to the query:
5z75A Artificial l-threonine 3-dehydrogenase designed by ancestral sequence reconstruction. (see paper)
27% identity, 59% coverage: 11:169/271 of query aligns to 4:166/306 of 5z75A
- binding nicotinamide-adenine-dinucleotide: G9 (= G16), C11 (≠ A18), G12 (= G19), Q13 (≠ G20), I14 (≠ L21), D35 (= D40), I36 (= I41), L49 (≠ V54), V51 (≠ L59), L72 (≠ F78), A73 (≠ G79), L76 (vs. gap), P113 (≠ A116), S115 (= S118), Y140 (= Y143), K144 (= K147)
Sites not aligning to the query:
6wj9B Udp-glcnac c4-epimerase mutant s121a/y146f from pseudomonas protegens in complex with udp-glcnac (see paper)
30% identity, 61% coverage: 11:174/271 of query aligns to 3:175/308 of 6wj9B
- active site: A119 (≠ S118), A120 (≠ N119), A121 (≠ H120), F144 (≠ Y143), K148 (= K147)
- binding nicotinamide-adenine-dinucleotide: G8 (= G16), G11 (= G19), F12 (≠ G20), I13 (≠ L21), D32 (= D40), D33 (≠ I41), S35 (≠ P43), T36 (≠ M44), G37 (≠ A45), D55 (= D58), A56 (≠ L59), L75 (≠ F78), A76 (≠ G79), A77 (≠ G80), S94 (≠ A93), A117 (= A116), A119 (≠ S118), F144 (≠ Y143), K148 (= K147), F171 (≠ I170), F172 (≠ G171), I174 (≠ S173)
- binding uridine-diphosphate-n-acetylglucosamine: V81 (= V83), N173 (≠ S172)
Sites not aligning to the query:
6wjaA Udp-glcnac c4-epimerase mutant s121a/y146f from pseudomonas protegens in complex with udp-galnac (see paper)
30% identity, 61% coverage: 11:174/271 of query aligns to 2:174/307 of 6wjaA
- active site: A118 (≠ S118), A119 (≠ N119), A120 (≠ H120), F143 (≠ Y143), K147 (= K147)
- binding nicotinamide-adenine-dinucleotide: G7 (= G16), G10 (= G19), F11 (≠ G20), I12 (≠ L21), D31 (= D40), D32 (≠ I41), S34 (≠ P43), T35 (≠ M44), G36 (≠ A45), A55 (≠ L59), L74 (≠ F78), A75 (≠ G79), A76 (≠ G80), S93 (≠ A93), F143 (≠ Y143), K147 (= K147), F170 (≠ I170), F171 (≠ G171), I173 (≠ S173)
- binding uridine-diphosphate-n-acetylgalactosamine: V80 (= V83), A120 (≠ H120), N172 (≠ S172)
Sites not aligning to the query:
5u4qB 1.5 angstrom resolution crystal structure of NAD-dependent epimerase from klebsiella pneumoniae in complex with NAD.
27% identity, 68% coverage: 11:193/271 of query aligns to 3:205/304 of 5u4qB
- binding nicotinamide-adenine-dinucleotide: G8 (= G16), G11 (= G19), F12 (≠ G20), I13 (≠ L21), D32 (≠ I36), N33 (≠ R37), N35 (≠ S39), Y38 (≠ A42), K43 (≠ A47), D61 (= D58), L62 (= L59), L83 (≠ F78), A84 (≠ G79), A85 (vs. gap), A123 (= A116), Y150 (= Y143), K154 (= K147), F177 (≠ I170), V180 (≠ S173), R186 (vs. gap), M189 (≠ P177)
2c59A Gdp-mannose-3', 5' -epimerase (arabidopsis thaliana), with gdp-alpha- d-mannose and gdp-beta-l-galactose bound in the active site. (see paper)
29% identity, 59% coverage: 11:171/271 of query aligns to 18:188/364 of 2c59A
- active site: S132 (= S118), A133 (≠ N119), C134 (≠ H120), Y163 (= Y143), K167 (= K147)
- binding guanosine-5'-diphosphate-beta-l-galactose: M91 (≠ F78), G92 (= G79), G93 (= G80), M94 (≠ V81), S132 (= S118), C134 (≠ H120)
- binding guanosine-5'-diphosphate-alpha-d-mannose: G92 (= G79), M94 (≠ V81), I97 (≠ V83), S132 (= S118), C134 (≠ H120), Y163 (= Y143)
- binding nicotinamide-adenine-dinucleotide: G23 (= G16), G26 (= G19), F27 (≠ G20), I28 (≠ L21), D47 (= D40), W48 (≠ I41), K49 (≠ A42), D67 (= D61), L68 (≠ K62), L87 (vs. gap), A89 (= A76), M91 (≠ F78), I111 (≠ G97), A130 (= A116), S132 (= S118), Y163 (= Y143), K167 (= K147)
Sites not aligning to the query:
- active site: 206
- binding guanosine-5'-diphosphate-beta-l-galactose: 191, 192, 206, 207, 210, 211, 214, 225, 230, 232, 266, 289, 290, 345
- binding guanosine-5'-diphosphate-alpha-d-mannose: 192, 206, 207, 210, 211, 214, 225, 230, 232, 266, 289, 290, 295, 345
- binding nicotinamide-adenine-dinucleotide: 190, 192, 193, 206
Q93VR3 GDP-mannose 3,5-epimerase; GDP-Man 3,5-epimerase; EC 5.1.3.18 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
29% identity, 59% coverage: 11:171/271 of query aligns to 29:199/377 of Q93VR3
- 34:60 (vs. 16:42, 26% identical) binding
- D58 (= D40) binding
- D78 (= D61) binding
- C145 (≠ H120) mutation to A: Loss of activity.; mutation to S: Strong reduction of activity.
- Y174 (= Y143) binding ; mutation to F: Loss of activity.
- K178 (= K147) binding ; mutation to R: Strong reduction of activity.
Sites not aligning to the query:
- 217 K→A: Loss of activity.
- 306 R→A: Strong reduction of activity.
7eprB Partial consensus l-threonine 3-dehydrogenase (c-change) (see paper)
28% identity, 59% coverage: 11:169/271 of query aligns to 3:166/310 of 7eprB
- binding nicotinamide-adenine-dinucleotide: G8 (= G16), G11 (= G19), Q12 (≠ G20), I13 (≠ L21), D34 (= D40), I35 (= I41), R36 (≠ A42), D50 (≠ M55), A51 (≠ P56), L72 (≠ I75), A73 (= A76), A74 (≠ H77), L90 (≠ A93), P113 (≠ A116), Y140 (= Y143), K144 (= K147)
Sites not aligning to the query:
2c54A Gdp-mannose-3', 5' -epimerase (arabidopsis thaliana),k178r, with gdp- beta-l-gulose and gdp-4-keto-beta-l-gulose bound in active site. (see paper)
29% identity, 59% coverage: 11:171/271 of query aligns to 17:187/362 of 2c54A
- active site: S131 (= S118), A132 (≠ N119), C133 (≠ H120), Y162 (= Y143), R166 (≠ K147)
- binding guanosine 5'-diphosphate-4-keto-beta-l-gulose: G91 (= G79), G92 (= G80), M93 (≠ V81), S131 (= S118)
- binding guanosine 5'-diphosphate-beta-l-gulose: G91 (= G79), G92 (= G80), M93 (≠ V81), S131 (= S118), C133 (≠ H120), Y162 (= Y143)
- binding nicotinamide-adenine-dinucleotide: G22 (= G16), G25 (= G19), F26 (≠ G20), I27 (≠ L21), D46 (= D40), W47 (≠ I41), K48 (≠ A42), V65 (≠ A60), D66 (= D61), L67 (≠ K62), L86 (vs. gap), A88 (= A76), M90 (≠ F78), I110 (≠ G97), A129 (= A116), S131 (= S118), Y162 (= Y143), R166 (≠ K147)
Sites not aligning to the query:
- active site: 205
- binding guanosine 5'-diphosphate-4-keto-beta-l-gulose: 190, 191, 205, 206, 209, 210, 213, 224, 229, 231, 265, 288, 289, 344
- binding guanosine 5'-diphosphate-beta-l-gulose: 190, 191, 205, 206, 209, 210, 213, 224, 229, 231, 265, 288, 289, 344
- binding nicotinamide-adenine-dinucleotide: 189, 191, 192, 205
Query Sequence
>GFF364 FitnessBrowser__psRCH2:GFF364
MTTTPTPQFGRLLLTGAAGGLGKELRERLQPYARIIRLSDIAPMAPAAGAHEEVMPCDLA
DKAAVHALCEGVDAIAHFGGVSVERSFEEILDANIRGTFHIYEAARLHGIKRVVFASSNH
VIGFYPQTETLDAHSPRRPDGYYGLSKSYGEDMANFYYDRYGIETVSIRIGSSFPEPANR
RMMSTWLSFRDLTELMRCALFTPAVGHTVVYGMSANRDVWWDNHLAAHLGFSAQDSSEVF
RDKVEQQPPYSADDPAGIYQGGAFVAAGPFD
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory