SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF3641 PGA1_262p00450 sugar ABC transporter, ATP-binding protein to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P04983 Ribose import ATP-binding protein RbsA; EC 7.5.2.7 from Escherichia coli (strain K12) (see paper)
40% identity, 91% coverage: 15:250/260 of query aligns to 4:238/501 of P04983

query
sites
P04983

L

L

V

L

E

Q

M

L

K

K

D

G

I

I

S

D

I

K

S

A

F

F

G

P

G

G

I

V

K

K

A

A

V

L

D

S

H

G

V

A

S

A

V

L

D

N

L

V

Y

Y

P

P

G

G

E

R

V

V

V

M

G

A

L

L

L

V

G

G

H

E

N

N

G

G

A

A

G

G

K
|
K

S

S

T

T

L

M

I

M

K

K

V

V

L

L

S

T

G

G

A

I

Y

Y

Q

T

M

R

D

D

A

A

G

G

E

T

I

L

R

L

V

W

N

L

G

G

D

K

K

E

V

T

E

T

I

F

T

T

N

G

P

P

R

K

D

S

A

S

R

Q

S

E

H

A

N

G

I

I

E

G

T

I

I

I

Y

H

Q

Q

T

E

L

L

A

N

L

L

A

I

D

P

N

Q

L

L

D

T

A

I

A

A

S

E

N

N

L

I

F

F

L

L

G

G

R

R

E

E

L

F

V

V

T

N

P

R

F

F

G

G

L

K

V

I

D

D

D

W

S

K

A

T

M

M

E

Y

A

A

E

E

C

A

R

D

K

K

I

L

M

L

N

A

R

K

L

L

N

N

P

L

N

R

F

F

Q

-

K

K

F

S

S

D

E

K

P

L

V

V

S

G

A

D

L

L

S

S

G

I

G

G

Q

D

R

Q

Q

Q

S

M

V

V

A

E

I

I

A

A

R

K

A

V

V

L

Y

S

F

F

N

E

A

S

K

K

I

V

L

I

I

I

M

M

D

D

E

E

P

P

T

T

A

D

A

A

L

L

G

T

P

D

H

T

E

E

T

T

Q

E

M

S

V

L

A

F

E

R

L

V

I

I

Q

R

Q

E

L

L

K

K

A

S

Q

Q

G

G

I

R

G

G

I

I

F

V

L

Y

I

I

D

S

H

H

D

R

V

M

N

K

A

E

V

I

M

F

E

E

L

I

C

C

D

D

R

D

A

V

S

T

V

V

M

F

K

R

N

D

G

G

Q

Q

L

F

V

I

G

A

T

E

V

R

D

E

I

V

D

A

D

S

V

L

T

T

D

E

D

D

D

S

L

L

L

I

S

E

M

M

I

M

I

V

K43 (= K54) mutation to R: Loss of transport.

1g6hA Crystal structure of the adp conformation of mj1267, an atp-binding cassette of an abc transporter (see paper)
30% identity, 92% coverage: 15:253/260 of query aligns to 4:254/254 of 1g6hA

query
sites
1g6hA

L

I

V

L

E

R

M

T

K

E

D

N

I

I

S

V

I

K

S

Y

F
|
F

G

G

G

E

I
x
F

K

K

A

A

V

L

D

D

H

G

V

V

S

S

V

I

D

S

L

V

Y

N

P

K

G

G

E

D

V

V

V

T

G

L

L

I

L

I

G

G

H

P

N
|
N

G
|
G

A

S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

I

I

K

N

V

V

L

I

S

T

G

G

A

F

Y

L

Q

K

M

A

D

D

A

E

G

G

E

-

I

-

R

R

V

V

N

Y

G

F

D

E

K

N

V

K

E

D

I

I

T

T

N

N

-

K

-

E

P

P

R

A

D

E

A

L

R

Y

S

H

H

Y

N

G

I

I

E

V

T

R

I

T

Y

F

Q

Q

T

T

L

P

A

Q

L

P

A

L

D

K

N

E

L

M

D

T

A

V

A

L

S

E

N

N

L

L

F

L

L

I

G

G

R

E

-

I

-

C

-

P

-

G

-

E

-

S

-

P

-

L

-

N

E

S

L

L

V

F

T

Y

P

K

F

K

G

W

L

I

V

P

D

K

D

E

S

E

A

E

M

M

E

V

A

E

E

K

C

A

R

F

K

K

I

I

M

L

N

E

R

F

L

L

N

K

P

L

N

S

F

-

Q

H

K

L

F

Y

S

D

E

R

P

K

V

A

S

G

A

E

L

L

S

S

G

G

G

G

Q

Q

R

M

Q

K

S

L

V

V

A

E

I

I

A

G

R

R

A

A

V

L

Y

M

F

T

N

N

A

P

K

K

I

M

L

I

I

V

M

M

D

D

E

E

P

P

T

I

A

A

A

G

L

V

G

A

P

P

H

G

E

L

T

A

Q

H

M

D

V

I

A

F

E

N

L

H

I

V

Q

L

Q

E

L

L

K

K

A

A

Q

K

G

G

I

I

G

T

I

F

F

L

L

I

I

I

D

E

H

H

D

R

V

L

N

D

A

I

V

V

M

L

E

N

L

Y

C

I

D

D

R

H

A

L

S

Y

V

V

M

M

K

F

N

N

G

G

Q

Q

L

I

V

I

-

A

-

E

-

G

-

R

G

G

T

E

V

E

D

E

I

I

D

K

D

N

V

V

T

L

D

S

D

D

D

P

L

K

L

V

S

V

M

E

I

I

I

Y

L

I

G

G

K

E

binding adenosine-5'-diphosphate: F14 (= F25), F17 (≠ I28), N39 (= N50), G40 (= G51), G42 (= G53), K43 (= K54), S44 (= S55), T45 (= T56)

1g9xB Characterization of the twinning structure of mj1267, an atp-binding cassette of an abc transporter (see paper)
30% identity, 92% coverage: 15:252/260 of query aligns to 4:253/253 of 1g9xB

query
sites
1g9xB

L

I

V

L

E

R

M

T

K

E

D

N

I

I

S

V

I

K

S

Y

F
|
F

G

G

G

E

I
x
F

K

K

A

A

V

L

D

D

H

G

V

V

S

S

V

I

D

S

L

V

Y

C

P

K

G

G

E

D

V

V

V

T

G

L

L

I

L

I

G

G

H

P

N

N

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

I

I

K

N

V

V

L

I

S

T

G

G

A

F

Y

L

Q

K

M

A

D

D

A

E

G

G

E

-

I

-

R

R

V

V

N

Y

G

F

D

E

K

N

V

K

E

D

I

I

T

T

N

N

-

K

-

E

P

P

R

A

D

E

A

L

R

Y

S

H

H

Y

N

G

I

I

E

V

T

R

I

T

Y

F

Q

Q

T

T

L

P

A

Q

L

P

A

L

D

K

N

E

L

M

D

T

A

V

A

L

S

E

N

N

L

L

F

L

L

I

G

G

R

E

-

I

-

N

-

P

-

G

-

E

-

S

-

P

-

L

-

N

E

S

L

L

V

F

T

Y

P

K

F

K

G

W

L

I

V

P

D

K

D

E

S

E

A

E

M

M

E

V

A

E

E

K

C

A

R

F

K

K

I

I

M

L

N

E

R

F

L

L

N

K

P

L

N

S

F

-

Q

H

K

L

F

Y

S

D

E

R

P

K

V

A

S

G

A

E

L

L

S

S

G

G

G

G

Q

Q

R

M

Q

K

S

L

V

V

A

E

I

I

A

G

R

R

A

A

V

L

Y

M

F

T

N

N

A

P

K

K

I

M

L

I

I

V

M

M

D

D

E
|
E

P

P

T

I

A

A

A

G

L

V

G

A

P

P

H

G

E

L

T

A

Q

H

M

D

V

I

A

F

E

N

L

H

I

V

Q

L

Q

E

L

L

K

K

A

A

Q

K

G

G

I

I

G

T

I

F

F

L

L

I

I

I

D

E

H

H

D

R

V

L

N

D

A

I

V

V

M

L

E

N

L

Y

C

I

D

D

R

H

A

L

S

Y

V

V

M

M

K

F

N

N

G

G

Q

Q

L

I

V

I

-

A

-

E

-

G

-

R

G

G

T

E

V

E

D

E

I

I

D

K

D

N

V

V

T

L

D

S

D

D

D

P

L

K

L

V

S

V

M

E

I

I

I

Y

L

I

G

G

binding adenosine-5'-diphosphate: F14 (= F25), F17 (≠ I28), G40 (= G51), S41 (≠ A52), G42 (= G53), K43 (= K54), S44 (= S55), T45 (= T56)
binding magnesium ion: S44 (= S55), E176 (= E179)

6s8nB Cryo-em structure of lptb2fgc in complex with lipopolysaccharide (see paper)
30% identity, 90% coverage: 19:253/260 of query aligns to 6:236/238 of 6s8nB

query
sites
6s8nB

K

K

D

N

I

L

S

A

I

K

S

A

F

Y

G

K

G

G

I

R

K

R

A

V

V

V

D

E

H

D

V

V

S

S

V

L

D

T

L

V

Y

N

P

S

G

G

E

E

V

I

V

V

G

G

L

L

L

L

G

G

H

P

N

N

G

G

A

A

G

G

K

K

S

T

T

T

L

T

I

F

K

Y

V

M

L

V

S

V

G

G

A

I

Y

V

Q

P

M

R

D

D

A

A

G

G

E

N

I

I

R

I

V

I

N

D

G

D

D

D

K

D

V

I

E

S

I

L

T

L

N

-

P

P

R

L

D

H

A

A

R

R

S

A

H

R

-

R

-

G

-

I

-

G

-

Y

-

L

-

P

N

Q

I

E

E

A

T

S

I

I

Y

F

Q
x
R

T

R

L

L

A

S

L

V

A

Y

D

D

N

N

L

L

D

M

A

A

A

V

S

-

N

-

L

-

F

-

L

-

G

-

R

-

E

-

L

-

V

-

T

-

P

-

F

L

G

Q

L

I

V

R

D

D

D

D

S

-

A

-

M

L

E

S

A

A

E

E

C

Q

R

R

K

E

I

-

M

-

N

D

R

R

L

A

N

N

P

E

N

L

F

M

Q

E

K

E

F

F

S

H

-

I

E

E

P

H

V

L

-

R

-

D

-

S

-
x
M

-
x
G

S
x
Q

A

S

L

L

S

S

G

G

G

G

Q

E

R

R

Q

R

S

R

V

V

A

E

I

I

A

A

R

R

A

A

V

L

Y

A

F

A

N

N

A

P

K

K

I

F

L

I

I

L

M

L

D

D

E

E

P

P

T

F

A

A

A

G

L

V

G

D

P

P

H

I

E

S

T

V

Q

I

M

D

V

I

A

K

E

R

L

I

I

I

Q

E

Q

H

L

L

K

R

A

D

Q

S

G

G

I

L

G

G

I

V

F

L

L

I

I

T

D

D

H

H

D

N

V

V

N

R

A

E

V

T

M

L

E

A

L

V

C

C

D

E

R

R

A

A

S

Y

V

I

M

V

K

S

N

Q

G

G

Q

H

L

L

V

I

G

A

T

H

V

G

D

T

I

P

D

T

D

E

V

I

T

L

D

Q

D

D

D

E

L

H

L

V

S

K

M

R

I

V

I

Y

L

L

G

G

K

E

binding 2-decyl-5,6-dimethoxy-3-methylcyclohexa-2,5-diene-1,4-dione: R90 (≠ Q97), M133 (vs. gap), G134 (vs. gap), Q135 (≠ S152)

6s8gA Cryo-em structure of lptb2fgc in complex with amp-pnp (see paper)
30% identity, 90% coverage: 19:253/260 of query aligns to 6:236/238 of 6s8gA

query
sites
6s8gA

K

K

D

N

I

L

S

A

I

K

S

A

F
x
Y

G

K

G

G

I
x
R

K

R

A

V

V

V

D

E

H

D

V

V

S

S

V

L

D

T

L

V

Y

N

P

S

G

G

E

E

V

I

V

V

G

G

L

L

L

L

G

G

H

P

N
|
N

G

G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

L

T

I

F

K

Y

V

M

L

V

S

V

G

G

A

I

Y

V

Q

P

M

R

D

D

A

A

G

G

E

N

I

I

R

I

V

I

N

D

G

D

D

D

K

D

V

I

E

S

I

L

T

L

N

-

P

P

R

L

D

H

A

A

R

R

S

A

H

R

-

R

-

G

-

I

-

G

-

Y

-

L

-

P

N
x
Q

I

E

E

A

T

S

I

I

Y

F

Q

R

T

R

L

L

A

S

L

V

A

Y

D

D

N

N

L

L

D

M

A

A

A

V

S

-

N

-

L

-

F

-

L

-

G

-

R

-

E

-

L

-

V

-

T

-

P

-

F

L

G

Q

L

I

V

R

D

D

D

D

S

-

A

-

M

L

E

S

A

A

E

E

C

Q

R

R

K

E

I

-

M

-

N

D

R

R

L

A

N

N

P

E

N

L

F

M

Q

E

K

E

F

F

S

H

-

I

E

E

P

H

V

L

-

R

-

D

-

S

-

M

-

G

S

Q

A
x
S

L

L

S
|
S

G

G

G

G

Q
x
E

R

R

Q

R

S

R

V

V

A

E

I

I

A

A

R

R

A

A

V

L

Y

A

F

A

N

N

A

P

K

K

I

F

L

I

I

L

M

L

D

D

E

E

P

P

T

F

A

A

A

G

L

V

G

D

P

P

H

I

E

S

T

V

Q

I

M

D

V

I

A

K

E

R

L

I

I

I

Q

E

Q

H

L

L

K

R

A

D

Q

S

G

G

I

L

G

G

I

V

F

L

L

I

I

T

D

D

H

H

D

N

V

V

N

R

A

E

V

T

M

L

E

A

L

V

C

C

D

E

R

R

A

A

S

Y

V

I

M

V

K

S

N

Q

G

G

Q

H

L

L

V

I

G

A

T

H

V

G

D

T

I

P

D

T

D

E

V

I

T

L

D

Q

D

D

D

E

L

H

L

V

S

K

M

R

I

V

I

Y

L

L

G

G

K

E

binding phosphoaminophosphonic acid-adenylate ester: Y12 (≠ F25), R15 (≠ I28), N37 (= N50), G40 (= G53), K41 (= K54), T42 (≠ S55), T43 (= T56), Q84 (≠ N91), S136 (≠ A153), S138 (= S155), E141 (≠ Q158)

6mhzA Vanadate trapped cryo-em structure of e.Coli lptb2fg transporter (see paper)
30% identity, 90% coverage: 19:252/260 of query aligns to 6:235/235 of 6mhzA

query
sites
6mhzA

K

K

D

N

I

L

S

A

I

K

S

A

F
x
Y

G

K

G

G

I

R

K

R

A

V

V

V

D

E

H

D

V

V

S

S

V

L

D

T

L

V

Y

N

P

S

G

G

E

E

V

I

V

V

G

G

L

L

L

L

G

G

H

P

N
|
N

G
|
G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

L

T

I

F

K

Y

V

M

L

V

S

V

G

G

A

I

Y

V

Q

P

M

R

D

D

A

A

G

G

E

N

I

I

R

I

V

I

N

D

G

D

D

D

K

D

V

I

E

S

I

L

T

L

N

-

P

P

R

L

D

H

A

A

R

R

S

A

H

R

-

R

-

G

-

I

-

G

-

Y

-

L

-

P

N
x
Q

I

E

E

A

T

S

I

I

Y

F

Q

R

T

R

L

L

A

S

L

V

A

Y

D

D

N

N

L

L

D

M

A

A

A

V

S

-

N

-

L

-

F

-

L

-

G

-

R

-

E

-

L

-

V

-

T

-

P

-

F

L

G

Q

L

I

V

R

D

D

D

D

S

-

A

-

M

L

E

S

A

A

E

E

C

Q

R

R

K

E

I

-

M

-

N

D

R

R

L

A

N

N

P

E

N

L

F

M

Q

E

K

E

F

F

S

H

-

I

E

E

P

H

V

L

-

R

-

D

-

S

-

M

-

G

S

Q

A
x
S

L

L

S
|
S

G
|
G

G
|
G

Q

E

R

R

Q

R

S

R

V

V

A

E

I

I

A

A

R

R

A

A

V

L

Y

A

F

A

N

N

A

P

K

K

I

F

L

I

I

L

M

L

D

D

E
|
E

P

P

T

F

A

A

A
x
G

L

V

G

D

P

P

H

I

E

S

T

V

Q

I

M

D

V

I

A

K

E

R

L

I

I

I

Q

E

Q

H

L

L

K

R

A

D

Q

S

G

G

I

L

G

G

I

V

F

L

L

I

I

T

D

D

H
|
H

D

N

V

V

N

R

A

E

V

T

M

L

E

A

L

V

C

C

D

E

R

R

A

A

S

Y

V

I

M

V

K

S

N

Q

G

G

Q

H

L

L

V

I

G

A

T

H

V

G

D

T

I

P

D

T

D

E

V

I

T

L

D

Q

D

D

D

E

L

H

L

V

S

K

M

R

I

V

I

Y

L

L

G

G

binding adp orthovanadate: Y12 (≠ F25), N37 (= N50), G38 (= G51), G40 (= G53), K41 (= K54), T42 (≠ S55), T43 (= T56), Q84 (≠ N91), S136 (≠ A153), S138 (= S155), G139 (= G156), G140 (= G157), E162 (= E179), G166 (≠ A183), H194 (= H211)

6mjpA Lptb(e163q)fgc from vibrio cholerae (see paper)
26% identity, 92% coverage: 15:254/260 of query aligns to 2:237/240 of 6mjpA

query
sites
6mjpA

L

I

V

L

E

K

M

A

K

Q

D

H

I

L

S

A

I

K

S

S

F

Y

G

K

G

K

I

R

K

K

A

V

V

V

D

S

H

D

V

V

S

S

V

L

D

Q

L

V

Y

E

P

S

G

G

E

Q

V

I

V

V

G

G

L

L

L

L

G

G

H

P

N

N

G

G

A

A

G

G

K

K

S

T

T

T

L

S

I

F

K

Y

V

M

L

I

S

V

G

G

A

L

Y

V

Q

A

M

R

D

D

A

E

G

G

E

T

I

I

R

T

V

I

N

D

G

D

D

N

K

D

V

I

E

S

I

I

T

L

N

-

P

P

R

M

D

H

A

S

R

R

S

S

H

R

-

M

-

G

-

I

-

G

-

Y

-

L

-

P

N

Q

I

E

E

A

T

S

I

I

Y

F

Q

R

T

K

L

L

A

S

L

V

A

E

D

D

N

N

L

I

D

M

A

A

A

-

S

-

N

-

L

V

F

L

L

Q

G

T

R

R

E

E

L

E

V

L

T

T

P

-

F

-

G

-

L

-

V

-

D

-

D

H

S
x
E

A

E

M

R

E

Q

A
x
D

E

K

C

L

R

E

K

D

I

L

M

L

N

E

R

E

L

F

N

H

P

I

N

Q

F

H

Q

I

K

R

F

K

S

S

E

A

P

G

V

M

S

-

A

A

L

L

S

S

G

G

G

G

Q

E

R

R

Q

R

S

R

V

V

A

E

I

I

A

A

R

R

A

A

V

L

Y

A

F

A

N

N

A

P

K

Q

I

F

L

I

I

L

M

L

D

D

E

Q

P

P

T

F

A

A

A

G

L

V

G

D

P

P

H

I

E

S

T

V

Q

I

M

D

V

I

A

K

E

K

L

I

I

I

Q

E

Q

H

L

L

K

R

A

D

Q

R

G

G

I

L

G

G

I

V

F

L

L

I

I

T

D

D

H

H

D

N

V

V

N

R

A

E

V

T

M

L

E

D

L

V

C

C

D

E

R

K

A

A

S

Y

V

I

M

V

K

S

N

Q

G

G

Q

R

L

L

V

I

G

A

T

E

V

G

D

T

I

P

D

Q

D

D

V

V

T

L

D

N

D

N

D

E

L

Q

L

V

S

K

M

Q

I

V

I

Y

L

L

G

G

K

E

R

Q

binding calcium ion: E111 (≠ S127), D115 (≠ A131)

6mbnA Lptb e163q in complex with atp (see paper)
29% identity, 90% coverage: 19:253/260 of query aligns to 7:237/241 of 6mbnA

query
sites
6mbnA

K

K

D

N

I

L

S

A

I

K

S

A

F
x
Y

G

K

G

G

I
x
R

K

R

A
x
V

V

V

D

E

H

D

V

V

S

S

V

L

D

T

L

V

Y

N

P

S

G

G

E

E

V

I

V

V

G

G

L

L

L

L

G

G

H

P

N
|
N

G
|
G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

L

T

I

F

K

Y

V

M

L

V

S

V

G

G

A

I

Y

V

Q

P

M

R

D

D

A

A

G

G

E

N

I

I

R

I

V

I

N

D

G

D

D

D

K

D

V

I

E

S

I

L

T

L

N

-

P

P

R

L

D

H

A

A

R

R

S

A

H

R

-

R

-

G

-

I

-

G

-

Y

-

L

-

P

N

Q

I

E

E

A

T

S

I

I

Y

F

Q

R

T

R

L

L

A

S

L

V

A

Y

D

D

N

N

L

L

D

M

A

A

A

V

S

-

N

-

L

-

F

-

L

-

G

-

R

-

E

-

L

-

V

-

T

-

P

-

F

L

G

Q

L

I

V

R

D

D

D

D

S

-

A

-

M

L

E

S

A

A

E

E

C

Q

R

R

K

E

I

-

M

-

N

D

R

R

L

A

N

N

P

E

N

L

F

M

Q

E

K

E

F

F

S

H

-

I

E

E

P

H

V

L

-

R

-

D

-

S

-

M

-

G

S

Q

A

S

L

L

S

S

G

G

G

G

Q

E

R

R

Q

R

S

R

V

V

A

E

I

I

A

A

R

R

A

A

V

L

Y

A

F

A

N

N

A

P

K

K

I

F

L

I

I

L

M

L

D

D

E

Q

P

P

T

F

A

A

A

G

L

V

G

D

P

P

H

I

E

S

T

V

Q

I

M

D

V

I

A

K

E

R

L

I

I

I

Q

E

Q

H

L

L

K

R

A

D

Q

S

G

G

I

L

G

G

I

V

F

L

L

I

I

T

D

D

H
|
H

D

N

V

V

N

R

A

E

V

T

M

L

E

A

L

V

C

C

D

E

R

R

A

A

S

Y

V

I

M

V

K

S

N

Q

G

G

Q

H

L

L

V

I

G

A

T

H

V

G

D

T

I

P

D

T

D

E

V

I

T

L

D

Q

D

D

D

E

L

H

L

V

S

K

M

R

I

V

I

Y

L

L

G

G

K

E

binding adenosine-5'-triphosphate: Y13 (≠ F25), R16 (≠ I28), V18 (≠ A30), N38 (= N50), G39 (= G51), G41 (= G53), K42 (= K54), T43 (≠ S55), T44 (= T56), H195 (= H211)

6b89A E. Coli lptb in complex with adp and novobiocin (see paper)
30% identity, 90% coverage: 19:251/260 of query aligns to 6:234/234 of 6b89A

query
sites
6b89A

K

K

D

N

I

L

S

A

I

K

S

A

F
x
Y

G

K

G

G

I
x
R

K

R

A
x
V

V

V

D

E

H

D

V

V

S

S

V

L

D

T

L

V

Y

N

P

S

G

G

E

E

V

I

V

V

G

G

L

L

L

L

G

G

H

P

N
|
N

G
|
G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

L

T

I

F

K

Y

V

M

L

V

S

V

G

G

A

I

Y

V

Q

P

M

R

D

D

A

A

G

G

E

N

I

I

R

I

V

I

N

D

G

D

D

D

K

D

V

I

E

S

I

L

T

L

N

-

P

P

R
x
L

D
x
H

A

A

R

R

S

A

H

R

-

R

-

G

-

I

-

G

-

Y

-

L

-
x
P

N
x
Q

I

E

E
x
A

T
x
S

I

I

Y
x
F

Q
x
R

T
x
R

L
|
L

A

S

L

V

A

Y

D

D

N

N

L

L

D

M

A

A

A
x
V

S

-

N

-

L

-

F

-

L

-

G

-

R

-

E

-

L

-

V

-

T

-

P

-

F

L

G

Q

L

I

V

R

D

D

D

D

S

-

A

-

M

L

E

S

A

A

E

E

C

Q

R

R

K

E

I

-

M

-

N

D

R

R

L

A

N

N

P

E

N

L

F

M

Q

E

K

E

F

F

S

H

-

I

E

E

P

H

V

L

-

R

-

D

-

S

-

M

-

G

S
x
Q

A

S

L

L

S

S

G

G

G

G

Q

E

R

R

Q

R

S

R

V

V

A

E

I

I

A

A

R
|
R

A

A

V

L

Y

A

F

A

N

N

A

P

K

K

I

F

L

I

I

L

M

L

D

D

E

E

P

P

T

F

A

A

A

G

L

V

G

D

P

P

H

I

E

S

T

V

Q

I

M

D

V

I

A

K

E

R

L

I

I

I

Q

E

Q

H

L

L

K

R

A

D

Q

S

G

G

I

L

G

G

I

V

F

L

L

I

I

T

D

D

H

H

D

N

V

V

N

R

A

E

V

T

M

L

E

A

L

V

C

C

D

E

R

R

A

A

S

Y

V

I

M

V

K

S

N

Q

G

G

Q

H

L

L

V

I

G

A

T

H

V

G

D

T

I

P

D

T

D

E

V

I

T

L

D

Q

D

D

D

E

L

H

L

V

S

K

M

R

I

V

I

Y

L

L

binding adenosine-5'-diphosphate: Y12 (≠ F25), R15 (≠ I28), V17 (≠ A30), N37 (= N50), G38 (= G51), G40 (= G53), K41 (= K54), T42 (≠ S55), T43 (= T56)
binding magnesium ion: T42 (≠ S55), Q84 (≠ N91)
binding novobiocin: L71 (≠ R85), H72 (≠ D86), P83 (vs. gap), A86 (≠ E93), S87 (≠ T94), F89 (≠ Y96), R90 (≠ Q97), R91 (≠ T98), L92 (= L99), V101 (≠ A108), Q135 (≠ S152), R149 (= R166)

4p31A Crystal structure of a selenomethionine derivative of e. Coli lptb in complex with adp-magensium (see paper)
30% identity, 90% coverage: 19:251/260 of query aligns to 6:234/234 of 4p31A

query
sites
4p31A

K

K

D

N

I

L

S

A

I

K

S

A

F
x
Y

G

K

G

G

I
x
R

K

R

A
x
V

V

V

D

E

H

D

V

V

S

S

V

L

D

T

L

V

Y

N

P

S

G

G

E

E

V

I

V

V

G

G

L

L

L

L

G

G

H

P

N

N

G
|
G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

L

T

I

F

K

Y

V

M

L

V

S

V

G

G

A

I

Y

V

Q

P

M

R

D

D

A

A

G

G

E

N

I

I

R

I

V

I

N

D

G

D

D

D

K

D

V

I

E

S

I

L

T

L

N

-

P

P

R

L

D

H

A

A

R

R

S

A

H

R

-

R

-

G

-

I

-

G

-

Y

-

L

-

P

N
x
Q

I

E

E

A

T

S

I

I

Y

F

Q

R

T

R

L

L

A

S

L

V

A

Y

D

D

N

N

L

L

D

M

A

A

A

V

S

-

N

-

L

-

F

-

L

-

G

-

R

-

E

-

L

-

V

-

T

-

P

-

F

L

G

Q

L

I

V

R

D

D

D

D

S

-

A

-

M

L

E

S

A

A

E

E

C

Q

R

R

K

E

I

-

M

-

N

D

R

R

L

A

N

N

P

E

N

L

F

M

Q

E

K

E

F

F

S

H

-

I

E

E

P

H

V

L

-

R

-

D

-

S

-

M

-

G

S

Q

A

S

L

L

S

S

G

G

G

G

Q

E

R

R

Q

R

S

R

V

V

A

E

I

I

A

A

R

R

A

A

V

L

Y

A

F

A

N

N

A

P

K

K

I

F

L

I

I

L

M

L

D

D

E

E

P

P

T

F

A

A

A

G

L

V

G

D

P

P

H

I

E

S

T

V

Q

I

M

D

V

I

A

K

E

R

L

I

I

I

Q

E

Q

H

L

L

K

R

A

D

Q

S

G

G

I

L

G

G

I

V

F

L

L

I

I

T

D

D

H

H

D

N

V

V

N

R

A

E

V

T

M

L

E

A

L

V

C

C

D

E

R

R

A

A

S

Y

V

I

M

V

K

S

N

Q

G

G

Q

H

L

L

V

I

G

A

T

H

V

G

D

T

I

P

D

T

D

E

V

I

T

L

D

Q

D

D

D

E

L

H

L

V

S

K

M

R

I

V

I

Y

L

L

binding adenosine-5'-diphosphate: Y12 (≠ F25), R15 (≠ I28), V17 (≠ A30), G38 (= G51), G40 (= G53), K41 (= K54), T42 (≠ S55), T43 (= T56)
binding magnesium ion: T42 (≠ S55), Q84 (≠ N91)

P30750 Methionine import ATP-binding protein MetN; EC 7.4.2.11 from Escherichia coli (strain K12) (see 3 papers)
32% identity, 82% coverage: 28:240/260 of query aligns to 18:228/343 of P30750

query
sites
P30750

I

I

K

Q

A

A

V

L

D

N

H

N

V

V

S

S

V

L

D

H

L

V

Y

P

P

A

G

G

E

Q

V

I

V

Y

G

G

L

V

L

I

G

G

H

A

N
x
S

G
|
G

A
|
A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

I

I

K

R

V

C

L

V

S

N

G

L

A

L

Y

E

Q

R

M

P

D

T

A

E

G

G

E

S

I

V

R

L

V

V

N

D

G

G

D

Q

K

E

V

L

-

T

-

T

-

L

-

S

-

E

-

S

E

E

I

L

T

T

N

K

P

A

R

R

D

-

A

-

R

-

S

-

H

R

N

Q

I

I

E

G

T

M

I

I

Y

F

Q

Q

T

H

L

F

A

N

L

L

A

L

D

S

N

S

L

R

D

T

A

V

A

F

S

G

N

N

L

V

F

A

L

L

G

P

R

L

E

E

L

L

V

-

T

-

P

-

F

-

G

-

L

-

V

-

D

D

D

N

S

T

A

P

M

K

E

D

A

E

E

V

C

K

R

R

K

R

I

V

M

T

N

E

R

L

L

L

N

S

P

L

N

V

F

G

-

L

-

G

Q

D

K

K

F

H

S

D

E

S

P

Y

V

P

S

S

A

N

L

L

S

S

G

G

G

G

Q

Q

R

K

Q

Q

S

R

V

V

A

A

I

I

A

A

R

R

A

A

V

L

Y

A

F

S

N

N

A

P

K

K

I

V

L

L

I

L

M

C

D

D

E
|
E

P

A

T

T

A

S

A

A

L

L

G

D

P

P

H

A

E

T

T

T

Q

R

M

S

V

I

A

L

E

E

L

L

I

L

Q

K

Q

D

L

I

K

N

A

R

Q

R

-

L

G

G

I

L

G

T

I

I

F

L

L

L

I

I

D

T

H

H

D

E

V

M

N

D

A

V

V

V

M

K

E

R

L

I

C

C

D

D

R

C

A

V

S

A

V

V

M

I

K

S

N

N

G

G

Q

E

L

L

V

I

G

E

T

Q

V

D

D

T

I

V

D

S

D

E

V

V

40:46 (vs. 50:56, 86% identical) binding
E166 (= E179) mutation to Q: Exhibits little ATPase activity.

Sites not aligning to the query:

278:283 binding
295 N→A: Reduces the binding of L-methionine to undetectable levels.
295:296 binding

3c4jA Abc protein artp in complex with atp-gamma-s
28% identity, 88% coverage: 15:242/260 of query aligns to 3:227/242 of 3c4jA

query
sites
3c4jA

L

M

V

I

E

D

M

V

K

H

D

Q

I

L

S

K

I

K

S

S

F
|
F

G

G

G

S

I

L

K

E

A
x
V

V

L

D

K

H

G

V

I

S

N

V

V

D

H

L

I

Y

R

P

E

G

G

E

E

V

V

V

V

G

V

L

V

L

I

G

G

H

P

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

I

L

K

R

V

C

L

L

S

N

G

L

A

L

Y

E

Q

D

M

F

D

D

A

E

G

G

E

E

I

I

R

I

V

I

N

D

G

G

D

I

-

N

-

L

K

K

V

A

E

K

I

D

T

T

N

N

P

L

R

N

D

K

A

V

R

R

S

E

H

-

N

E

I

V

E

G

T

M

I

V

Y

F

Q

Q

T

R

L

F

A

N

L

L

A

F

D

P

N

H

L

M

D

T

A

V

A

L

S

N

N

N

L

I

F

T

L

L

G

A

R

P

E

M

L

K

V

V

T

R

P

K

F

W

G

-

L

-

V

-

D

P

D

R

S

E

A

K

M

A

E

E

A

A

E

K

C

A

R

M

K

E

I

L

M

L

N

D

R

K

L

V

N

G

P

L

N

K

F

D

Q

K

K

A

F

H

S

A

E

Y

P

P

V

-

S

D

A

S

L

L

S

S

G

G

G

G

Q

Q

R

A

Q

Q

S

R

V

V

A

A

I

I

A

A

R

R

A

A

V

L

Y

A

F

M

N

E

A

P

K

K

I

I

L

M

I

L

M

F

D

D

E

E

P

P

T

T

A

S

A

A

L

L

G

D

P

P

H

E

E

M

T

V

Q

G

M

E

V

V

A

L

E

S

L

V

I

M

Q

K

Q

Q

L

L

K

A

A

N

Q

E

G

G

I

M

G

T

I

M

F

V

L

V

I

V

D

T

H

H

D

E

V

M

N

G

A

F

V

A

M

R

E

E

L

V

C

G

D

D

R

R

A

V

S

L

V

F

M

M

K

D

N

G

G

G

Q

Y

L

I

V

I

G

E

T

E

V

G

D

K

I

P

D

E

D

D

V

L

T

F

D

D

binding phosphothiophosphoric acid-adenylate ester: F13 (= F25), V18 (≠ A30), S38 (≠ N50), G39 (= G51), S40 (≠ A52), G41 (= G53), K42 (= K54), S43 (= S55), T44 (= T56)

3c41J Abc protein artp in complex with amp-pnp/mg2+
28% identity, 88% coverage: 15:242/260 of query aligns to 3:227/242 of 3c41J

query
sites
3c41J

L

M

V

I

E

D

M

V

K

H

D

Q

I

L

S

K

I

K

S

S

F
|
F

G

G

G

S

I

L

K

E

A
x
V

V

L

D

K

H

G

V

I

S

N

V

V

D

H

L

I

Y

R

P

E

G

G

E

E

V

V

V

V

G

V

L

V

L

I

G

G

H

P

N
x
S

G
|
G

A

S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

I

L

K

R

V

C

L

L

S

N

G

L

A

L

Y

E

Q

D

M

F

D

D

A

E

G

G

E

E

I

I

R

I

V

I

N

D

G

G

D

I

-

N

-

L

K

K

V

A

E

K

I

D

T

T

N

N

P

L

R

N

D

K

A

V

R

R

S

E

H

-

N

E

I

V

E

G

T

M

I

V

Y

F

Q

Q

T

R

L

F

A

N

L

L

A

F

D

P

N

H

L

M

D

T

A

V

A

L

S

N

N

N

L

I

F

T

L

L

G

A

R

P

E

M

L

K

V

V

T

R

P

K

F

W

G

-

L

-

V

-

D

P

D

R

S

E

A

K

M

A

E

E

A

A

E

K

C

A

R

M

K

E

I

L

M

L

N

D

R

K

L

V

N

G

P

L

N

K

F

D

Q

K

K

A

F

H

S

A

E

Y

P

P

V

-

S

D

A

S

L

L

S

S

G

G

G

G

Q

Q

R

A

Q

Q

S

R

V

V

A

A

I

I

A

A

R

R

A

A

V

L

Y

A

F

M

N

E

A

P

K

K

I

I

L

M

I

L

M

F

D
|
D

E

E

P

P

T

T

A

S

A

A

L

L

G

D

P

P

H

E

E

M

T

V

Q

G

M

E

V

V

A

L

E

S

L

V

I

M

Q

K

Q

Q

L

L

K

A

A

N

Q

E

G

G

I

M

G

T

I

M

F

V

L

V

I

V

D

T

H

H

D

E

V

M

N

G

A

F

V

A

M

R

E

E

L

V

C

G

D

D

R

R

A

V

S

L

V

F

M

M

K

D

N

G

G

G

Q

Y

L

I

V

I

G

E

T

E

V

G

D

K

I

P

D

E

D

D

V

L

T

F

D

D

binding phosphoaminophosphonic acid-adenylate ester: F13 (= F25), V18 (≠ A30), S38 (≠ N50), G39 (= G51), G41 (= G53), K42 (= K54), S43 (= S55), T44 (= T56)
binding magnesium ion: S43 (= S55), D163 (= D178)

2olkA Abc protein artp in complex with adp-beta-s
28% identity, 88% coverage: 15:242/260 of query aligns to 3:227/242 of 2olkA

query
sites
2olkA

L

M

V

I

E

D

M

V

K

H

D

Q

I

L

S

K

I

K

S

S

F
|
F

G

G

G

S

I

L

K

E

A
x
V

V

L

D

K

H

G

V

I

S

N

V

V

D

H

L

I

Y

R

P

E

G

G

E

E

V

V

V

V

G

V

L

V

L

I

G

G

H

P

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

I

L

K

R

V

C

L

L

S

N

G

L

A

L

Y

E

Q

D

M

F

D

D

A

E

G

G

E

E

I

I

R

I

V

I

N

D

G

G

D

I

-

N

-

L

K

K

V

A

E

K

I

D

T

T

N

N

P

L

R

N

D

K

A

V

R

R

S

E

H

-

N

E

I

V

E

G

T

M

I

V

Y

F

Q

Q

T

R

L

F

A

N

L

L

A

F

D

P

N

H

L

M

D

T

A

V

A

L

S

N

N

N

L

I

F

T

L

L

G

A

R

P

E

M

L

K

V

V

T

R

P

K

F

W

G

-

L

-

V

-

D

P

D

R

S

E

A

K

M

A

E

E

A

A

E

K

C

A

R

M

K

E

I

L

M

L

N

D

R

K

L

V

N

G

P

L

N

K

F

D

Q

K

K

A

F

H

S

A

E

Y

P

P

V

-

S

D

A

S

L

L

S

S

G

G

G

G

Q

Q

R

A

Q

Q

S

R

V

V

A

A

I

I

A

A

R

R

A

A

V

L

Y

A

F

M

N

E

A

P

K

K

I

I

L

M

I

L

M

F

D

D

E

E

P

P

T

T

A

S

A

A

L

L

G

D

P

P

H

E

E

M

T

V

Q

G

M

E

V

V

A

L

E

S

L

V

I

M

Q

K

Q

Q

L

L

K

A

A

N

Q

E

G

G

I

M

G

T

I

M

F

V

L

V

I

V

D

T

H

H

D

E

V

M

N

G

A

F

V

A

M

R

E

E

L

V

C

G

D

D

R

R

A

V

S

L

V

F

M

M

K

D

N

G

G

G

Q

Y

L

I

V

I

G

E

T

E

V

G

D

K

I

P

D

E

D

D

V

L

T

F

D

D

binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: F13 (= F25), V18 (≠ A30), S38 (≠ N50), G39 (= G51), S40 (≠ A52), G41 (= G53), K42 (= K54), S43 (= S55), T44 (= T56)

2oljA Abc protein artp in complex with adp/mg2+
28% identity, 88% coverage: 15:242/260 of query aligns to 3:227/242 of 2oljA

query
sites
2oljA

L

M

V

I

E

D

M

V

K

H

D

Q

I

L

S

K

I

K

S

S

F
|
F

G

G

G

S

I

L

K

E

A
x
V

V

L

D

K

H

G

V

I

S

N

V

V

D

H

L

I

Y

R

P

E

G

G

E

E

V

V

V

V

G

V

L

V

L

I

G

G

H

P

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

I

L

K

R

V

C

L

L

S

N

G

L

A

L

Y

E

Q

D

M

F

D

D

A

E

G

G

E

E

I

I

R

I

V

I

N

D

G

G

D

I

-

N

-

L

K

K

V

A

E

K

I

D

T

T

N

N

P

L

R

N

D

K

A

V

R

R

S

E

H

-

N

E

I

V

E

G

T

M

I

V

Y

F

Q

Q

T

R

L

F

A

N

L

L

A

F

D

P

N

H

L

M

D

T

A

V

A

L

S

N

N

N

L

I

F

T

L

L

G

A

R

P

E

M

L

K

V

V

T

R

P

K

F

W

G

-

L

-

V

-

D

P

D

R

S

E

A

K

M

A

E

E

A

A

E

K

C

A

R

M

K

E

I

L

M

L

N

D

R

K

L

V

N

G

P

L

N

K

F

D

Q

K

K

A

F

H

S

A

E

Y

P

P

V

-

S

D

A

S

L

L

S

S

G

G

G

G

Q

Q

R

A

Q

Q

S

R

V

V

A

A

I

I

A

A

R

R

A

A

V

L

Y

A

F

M

N

E

A

P

K

K

I

I

L

M

I

L

M

F

D

D

E

E

P

P

T

T

A

S

A

A

L

L

G

D

P

P

H

E

E

M

T

V

Q

G

M

E

V

V

A

L

E

S

L

V

I

M

Q

K

Q

Q

L

L

K

A

A

N

Q

E

G

G

I

M

G

T

I

M

F

V

L

V

I

V

D

T

H

H

D

E

V

M

N

G

A

F

V

A

M

R

E

E

L

V

C

G

D

D

R

R

A

V

S

L

V

F

M

M

K

D

N

G

G

G

Q

Y

L

I

V

I

G

E

T

E

V

G

D

K

I

P

D

E

D

D

V

L

T

F

D

D

binding adenosine-5'-diphosphate: F13 (= F25), V18 (≠ A30), S38 (≠ N50), G39 (= G51), S40 (≠ A52), G41 (= G53), K42 (= K54), S43 (= S55), T44 (= T56)

6b8bA E. Coli lptb in complex with adp and a novobiocin derivative (see paper)
31% identity, 82% coverage: 19:232/260 of query aligns to 6:215/233 of 6b8bA

query
sites
6b8bA

K

K

D

N

I

L

S

A

I

K

S

A

F
x
Y

G

K

G

G

I
x
R

K

R

A
x
V

V

V

D

E

H

D

V

V

S

S

V

L

D

T

L

V

Y

N

P

S

G

G

E

E

V

I

V

V

G

G

L

L

L

L

G

G

H

P

N

N

G
|
G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

L

T

I

F

K

Y

V

M

L

V

S

V

G

G

A

I

Y

V

Q

P

M

R

D

D

A

A

G

G

E

N

I

I

R

I

V

I

N

D

G

D

D

D

K

D

V

I

E

S

I

L

T

L

N

-

P

P

R

L

D

H

A

A

R

R

S

A

H

R

-

R

-

G

-

I

-

G

-

Y

-

L

-

P

N

Q

I

E

E

A

T

S

I

I

Y
x
F

Q
x
R

T
x
R

L

L

A

S

L

V

A

Y

D

D

N

N

L

L

D

M

A

A

A

V

S

-

N

-

L

-

F

-

L

-

G

-

R

-

E

-

L

-

V

-

T

-

P

-

F

L

G

Q

L

I

V

R

D

D

D

D

S

-

A

-

M

L

E

S

A

A

E

E

C

Q

R

R

K

E

I

-

M

-

N

D

R

R

L

A

N

N

P

E

N

L

F

M

Q

E

K

E

F

F

S

H

-

I

E

E

P

H

V

L

-

R

-

D

-

S

-

M

-

G

S

Q

A

S

L

L

S

S

G

G

G

G

Q

E

R

R

Q

R

S

R

V

V

A

E

I

I

A

A

R

R

A

A

V

L

Y

A

F

A

N

N

A

P

K

K

I

F

L

I

I

L

M

L

D

D

E

E

P

P

T

F

A

A

A

G

L

V

G

D

P

P

H

I

E

S

T

V

Q

I

M

D

V

I

A

K

E

R

L

I

I

I

Q

E

Q

H

L

L

K

R

A

D

Q

S

G

G

I

L

G

G

I

V

F

L

L

I

I

T

D

D

H

H

D

N

V

V

N

R

A

E

V

T

M

L

E

A

L

V

C

C

D

E

R

R

A

A

S

Y

V

I

M

V

K

S

N

Q

G

G

Q

H

L

L

V

I

binding adenosine-5'-diphosphate: Y12 (≠ F25), R15 (≠ I28), V17 (≠ A30), G38 (= G51), G40 (= G53), K41 (= K54), T42 (≠ S55), T43 (= T56)
binding (3s,5s,7s)-N-{7-[(3-O-carbamoyl-6-deoxy-5-methyl-4-O-methyl-beta-D-gulopyranosyl)oxy]-4-hydroxy-8-methyl-2-oxo-2H-1-benzopyran-3-yl}tricyclo[3.3.1.1~3,7~]decane-1-carboxamide: F89 (≠ Y96), R90 (≠ Q97), R91 (≠ T98)

P75831 Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- from Escherichia coli (strain K12) (see paper)
29% identity, 85% coverage: 13:232/260 of query aligns to 2:222/648 of P75831

query
sites
P75831

T

T

P

P

L

L

V

L

E

E

M

L

K

K

D

D

I

I

S

R

I

R

S

S

F

Y

G

P

G

A

-

G

-

D

-

E

-

Q

I

V

K

E

A

V

V

L

D

K

H

G

V

I

S

S

V

L

D

D

L

I

Y

Y

P

A

G

G

E

E

V

M

V

V

G

A

L

I

L

V

G

G

H

A

N

S

G

G

A

S

G

G

K
|
K

S

S

T

T

L

L

I

M

K

N

V

I

L

L

S

G

G

C

A

L

Y

D

Q

K

M

A

D

T

A

S

G

G

E

T

I

Y

R

R

V

V

N

A

G

G

D

Q

K

D

V

V

E

A

I

T

T

L

N

D

P

A

R

-

D

D

A

A

-

L

-

A

-

Q

-

L

R

R

S

R

H

E

N

H

I

F

E

G

T

F

I

I

Y

F

Q

Q

T

R

L

Y

A

H

L

L

A

L

D

S

N

H

L

L

D

T

A

A

A

E

S

Q

N

N

L

V

F

-

L

-

G

-

R

-

E

E

L

V

V

P

T

A

P

V

F

Y

G

A

L

G

V

L

D

E

D

R

S

K

A

Q

M

R

E

L

A

L

E

R

C

A

R

Q

K

E

I

L

M

L

N

Q

R

R

L

L

N

G

P

L

N

E

F

D

Q

R

K

T

F

E

S

Y

E

Y

P

P

V

-

S

A

A

Q

L

L

S

S

G

G

G

G

Q

Q

R

Q

Q

Q

S

R

V

V

A

S

I

I

A

A

R

R

A

A

V

L

Y

M

F

N

N

G

A

G

K

Q

I

V

L

I

I

L

M

A

D
|
D

E

E

P

P

T

T

A

G

A

A

L

L

G

D

P

S

H

H

E

S

T

G

Q

E

M

E

V

V

A

M

E

A

L

I

I

L

Q

H

Q

Q

L

L

K

R

A

D

Q

R

G

G

I

H

G

T

I

V

F

I

L

I

I

V

D

T

H

H

D

D

V

P

N

Q

A

V

V

A

M

A

E

Q

L

-

C

A

D

E

R

R

A

V

S

I

V

E

M

I

K

R

N

D

G

G

Q

E

L

I

V

V

K47 (= K54) mutation to L: Lack of activity.
D169 (= D178) mutation to N: Lack of activity.

6cvlD Crystal structure of the escherichia coli atpgs-bound metni methionine abc transporter in complex with its metq binding protein (see paper)
32% identity, 82% coverage: 28:240/260 of query aligns to 19:229/344 of 6cvlD

query
sites
6cvlD

I
|
I

K

Q

A

A

V

L

D

N

H

N

V

V

S

S

V

L

D

H

L

V

Y

P

P

A

G

G

E

Q

V

I

V

Y

G

G

L

V

L

I

G

G

H

A

N
x
S

G
|
G

A
|
A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

I

I

K

R

V

C

L

V

S

N

G

L

A

L

Y

E

Q

R

M

P

D

T

A

E

G

G

E

S

I

V

R

L

V

V

N

D

G

G

D

Q

K

E

V

L

-

T

-

T

-

L

-

S

-

E

-

S

E

E

I

L

T

T

N

K

P

A

R

R

D

-

A

-

R

-

S

-

H

R

N

Q

I

I

E

G

T

M

I

I

Y

F

Q

Q

T

H

L

F

A

N

L

L

A

L

D

S

N

S

L

R

D

T

A

V

A

F

S

G

N

N

L

V

F

A

L

L

G

P

R

L

E

E

L

L

V

-

T

-

P

-

F

-

G

-

L

-

V

-

D

D

D

N

S

T

A

P

M

K

E

D

A

E

E

V

C

K

R

R

K

R

I

V

M

T

N

E

R

L

L

L

N

S

P

L

N

V

F

G

-

L

-

G

Q

D

K

K

F

H

S

D

E

S

P

Y

V

P

S

S

A
x
N

L

L

S
|
S

G

G

G

G

Q
|
Q

R

K

Q

Q

S

R

V

V

A

A

I

I

A

A

R

R

A

A

V

L

Y

A

F

S

N

N

A

P

K

K

I

V

L

L

I

L

M

C

D

D

E

Q

P

A

T

T

A

S

A

A

L

L

G

D

P

P

H

A

E

T

T

T

Q

R

M

S

V

I

A

L

E

E

L

L

I

L

Q

K

Q

D

L

I

K

N

A

R

Q

R

-

L

G

G

I

L

G

T

I

I

F

L

L

L

I

I

D

T

H
|
H

D

E

V

M

N

D

A

V

V

V

M

K

E

R

L

I

C

C

D

D

R

C

A

V

S

A

V

V

M

I

K

S

N

N

G

G

Q

E

L

L

V

I

G

E

T

Q

V

D

D

T

I

V

D

S

D

E

V

V

binding phosphothiophosphoric acid-adenylate ester: I19 (= I28), S41 (≠ N50), G42 (= G51), A43 (= A52), G44 (= G53), K45 (= K54), S46 (= S55), T47 (= T56), N141 (≠ A153), S143 (= S155), Q146 (= Q158), H200 (= H211)

Sites not aligning to the query:

binding phosphothiophosphoric acid-adenylate ester: 12, 14

3tuzC Inward facing conformations of the metni methionine abc transporter: cy5 semet soak crystal form (see paper)
32% identity, 82% coverage: 28:240/260 of query aligns to 19:229/344 of 3tuzC

query
sites
3tuzC

I

I

K

Q

A

A

V

L

D

N

H

N

V

V

S

S

V

L

D

H

L

V

Y

P

P

A

G

G

E

Q

V

I

V

Y

G

G

L

V

L

I

G

G

H

A

N

S

G
|
G

A

A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

I

I

K

R

V

C

L

V

S

N

G

L

A

L

Y

E

Q

R

M

P

D

T

A

E

G

G

E

S

I

V

R

L

V

V

N

D

G

G

D

Q

K

E

V

L

-

T

-

T

-

L

-

S

-

E

-

S

E

E

I

L

T

T

N

K

P

A

R

R

D

-

A

-

R

-

S

-

H

R

N

Q

I

I

E

G

T

M

I

I

Y

F

Q

Q

T

H

L

F

A

N

L

L

A

L

D

S

N

S

L

R

D

T

A

V

A

F

S

G

N

N

L

V

F

A

L

L

G

P

R

L

E

E

L

L

V

-

T

-

P

-

F

-

G

-

L

-

V

-

D

D

D

N

S

T

A

P

M

K

E

D

A

E

E

V

C

K

R

R

K

R

I

V

M

T

N

E

R

L

L

L

N

S

P

L

N

V

F

G

-

L

-

G

Q

D

K

K

F

H

S

D

E

S

P

Y

V

P

S

S

A

N

L

L

S

S

G

G

G

G

Q

Q

R

K

Q

Q

S

R

V

V

A

A

I

I

A

A

R

R

A

A

V

L

Y

A

F

S

N

N

A

P

K

K

I

V

L

L

I

L

M

C

D

D

E

Q

P

A

T

T

A

S

A

A

L

L

G

D

P

P

H

A

E

T

T

T

Q

R

M

S

V

I

A

L

E

E

L

L

I

L

Q

K

Q

D

L

I

K

N

A

R

Q

R

-

L

G

G

I

L

G

T

I

I

F

L

L

L

I

I

D

T

H

H

D

E

V

M

N

D

A

V

V

V

M

K

E

R

L

I

C

C

D

D

R

C

A

V

S

A

V

V

M

I

K

S

N

N

G

G

Q

E

L

L

V

I

G

E

T

Q

V

D

D

T

I

V

D

S

D

E

V

V

binding adenosine-5'-diphosphate: G42 (= G51), G44 (= G53), K45 (= K54), S46 (= S55), T47 (= T56)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12, 14
binding selenomethionine: 274, 279, 281, 282, 283, 284, 311, 313

3tuiC Inward facing conformations of the metni methionine abc transporter: cy5 native crystal form (see paper)
32% identity, 82% coverage: 28:240/260 of query aligns to 19:229/344 of 3tuiC

query
sites
3tuiC

I

I

K

Q

A

A

V

L

D

N

H

N

V

V

S

S

V

L

D

H

L

V

Y

P

P

A

G

G

E

Q

V

I

V

Y

G

G

L

V

L

I

G

G

H

A

N

S

G
|
G

A
|
A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

I

I

K

R

V

C

L

V

S

N

G

L

A

L

Y

E

Q

R

M

P

D

T

A

E

G

G

E

S

I

V

R

L

V

V

N

D

G

G

D

Q

K

E

V

L

-

T

-

T

-

L

-

S

-

E

-

S

E

E

I

L

T

T

N

K

P

A

R

R

D

-

A

-

R

-

S

-

H

R

N

Q

I

I

E

G

T

M

I

I

Y

F

Q

Q

T

H

L

F

A

N

L

L

A

L

D

S

N

S

L

R

D

T

A

V

A

F

S

G

N

N

L

V

F

A

L

L

G

P

R

L

E

E

L

L

V

-

T

-

P

-

F

-

G

-

L

-

V

-

D

D

D

N

S

T

A

P

M

K

E

D

A

E

E

V

C

K

R

R

K

R

I

V

M

T

N

E

R

L

L

L

N

S

P

L

N

V

F

G

-

L

-

G

Q

D

K

K

F

H

S

D

E

S

P

Y

V

P

S

S

A

N

L

L

S

S

G

G

G

G

Q

Q

R

K

Q

Q

S

R

V

V

A

A

I

I

A

A

R

R

A

A

V

L

Y

A

F

S

N

N

A

P

K

K

I

V

L

L

I

L

M

C

D

D

E

Q

P

A

T

T

A

S

A

A

L

L

G

D

P

P

H

A

E

T

T

T

Q

R

M

S

V

I

A

L

E

E

L

L

I

L

Q

K

Q

D

L

I

K

N

A

R

Q

R

-

L

G

G

I

L

G

T

I

I

F

L

L

L

I

I

D

T

H

H

D

E

V

M

N

D

A

V

V

V

M

K

E

R

L

I

C

C

D

D

R

C

A

V

S

A

V

V

M

I

K

S

N

N

G

G

Q

E

L

L

V

I

G

E

T

Q

V

D

D

T

I

V

D

S

D

E

V

V

binding adenosine-5'-diphosphate: G42 (= G51), A43 (= A52), G44 (= G53), K45 (= K54), S46 (= S55), T47 (= T56)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12

Query Sequence

>GFF3641 PGA1_262p00450 sugar ABC transporter, ATP-binding protein
MSTAKELRAAGATPLVEMKDISISFGGIKAVDHVSVDLYPGEVVGLLGHNGAGKSTLIKV
LSGAYQMDAGEIRVNGDKVEITNPRDARSHNIETIYQTLALADNLDAASNLFLGRELVTP
FGLVDDSAMEAECRKIMNRLNPNFQKFSEPVSALSGGQRQSVAIARAVYFNAKILIMDEP
TAALGPHETQMVAELIQQLKAQGIGIFLIDHDVNAVMELCDRASVMKNGQLVGTVDIDDV
TDDDLLSMIILGKRPGEAAA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory