SitesBLAST
Comparing GFF3683 HP15_3625 choline dehydrogenase to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
8b7sA Crystal structure of the chloramphenicol-inactivating oxidoreductase from novosphingobium sp (see paper)
37% identity, 94% coverage: 4:529/561 of query aligns to 3:451/458 of 8b7sA
- binding flavin-adenine dinucleotide: G11 (= G12), G13 (= G14), S14 (= S15), A15 (= A16), E35 (= E36), A36 (≠ T37), W47 (= W63), P65 (= P81), G67 (= G83), V180 (= V221), A214 (= A255), G215 (= G256), A218 (≠ G259), T270 (≠ D332), Y391 (= Y469), A424 (≠ S502), I435 (≠ L513), N436 (= N514)
5nccA Structure of fatty acid photodecarboxylase in complex with fad and palmitic acid (see paper)
35% identity, 95% coverage: 4:537/561 of query aligns to 22:578/578 of 5nccA
- active site: R347 (≠ L321), L420 (≠ I383), I421 (≠ G384), S507 (≠ A468), A509 (≠ H470), G552 (= G511), Q553 (≠ N512)
- binding flavin-adenine dinucleotide: G30 (= G12), G32 (= G14), T33 (≠ S15), A34 (= A16), L53 (= L35), E54 (= E36), A55 (≠ T37), F74 (≠ N57), W80 (= W63), A98 (≠ P81), G100 (= G83), G105 (= G88), S106 (= S89), N110 (= N93), A111 (≠ G94), T112 (≠ M95), L113 (≠ V96), V238 (= V221), A278 (= A255), H282 (≠ G259), L286 (= L263), N508 (≠ Y469), Q553 (≠ N512), T554 (≠ L513), G555 (≠ N514), V558 (≠ T517)
6yrvAAA structure of fap after illumination at 100k (see paper)
35% identity, 96% coverage: 4:539/561 of query aligns to 6:571/573 of 6yrvAAA
- binding carbon dioxide: R375 (≠ H360), N499 (≠ Y469)
- binding flavin-adenine dinucleotide: G14 (= G12), G16 (= G14), T17 (≠ S15), A18 (= A16), L37 (= L35), E38 (= E36), A39 (≠ T37), F58 (≠ N57), W64 (= W63), A82 (≠ P81), G89 (= G88), S90 (= S89), N94 (= N93), A95 (≠ G94), T96 (≠ M95), L97 (≠ V96), M191 (≠ T190), V222 (= V221), C264 (≠ S254), A265 (= A255), G266 (= G256), H269 (≠ G259), N499 (≠ Y469), A534 (≠ S502), Q544 (≠ N512), T545 (≠ L513), G546 (≠ N514)
- binding heptadecane: V377 (≠ L362), G379 (≠ A364), M380 (vs. gap), G386 (= G370), T389 (≠ A373), Y390 (≠ F374), F393 (vs. gap), T408 (≠ Q380), Q410 (≠ H382)
A0A248QE08 Fatty acid photodecarboxylase, chloroplastic; CvFAP; EC 4.1.1.106 from Chlorella variabilis (Green alga) (see paper)
35% identity, 95% coverage: 4:534/561 of query aligns to 82:642/654 of A0A248QE08
- TA 93:94 (≠ SA 15:16) binding
- E114 (= E36) binding
- L162 (≠ V85) binding
- S166 (= S89) binding
- NATL 170:173 (≠ NGMV 93:96) binding
- V298 (= V221) binding
- C432 (vs. gap) binding
- R451 (≠ H360) binding
- Y466 (≠ F374) binding
- Q486 (≠ H382) binding
- G622 (≠ N514) binding
3ljpA Crystal structure of choline oxidase v464a mutant (see paper)
34% identity, 94% coverage: 1:529/561 of query aligns to 9:527/530 of 3ljpA
- active site: I333 (= I323), P377 (≠ I383), N378 (≠ G384), A464 (= A468), H466 (= H470), V509 (≠ G511), N510 (= N512)
- binding dihydroflavine-adenine dinucleotide: G22 (= G14), S23 (= S15), E44 (= E36), A45 (≠ T37), W71 (= W63), R89 (= R82), A90 (≠ G83), G95 (= G88), C96 (≠ S89), H99 (≠ I92), N100 (= N93), S101 (≠ G94), I103 (≠ V96), A232 (≠ V221), T269 (≠ A255), D273 (≠ G259), Y465 (= Y469), H466 (= H470), D499 (= D501), A500 (≠ S502), N510 (= N512), P511 (≠ L513), N512 (= N514), V515 (≠ T517)
2jbvA Crystal structure of choline oxidase reveals insights into the catalytic mechanism (see paper)
33% identity, 94% coverage: 1:529/561 of query aligns to 9:527/527 of 2jbvA
- active site: I333 (= I323), P377 (≠ I383), N378 (≠ G384), V464 (≠ A468), H466 (= H470), V509 (≠ G511), N510 (= N512)
- binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl (2R,3S,4S)-5-[(4aS,10aR)-7,8-dimethyl-2,4-dioxo-1,3,4,4a,5,10a-hexahydrobenzo[g]pteridin-10(2H)-yl]-2,3,4-trihydroxypentyl dihydrogen diphosphate: G22 (= G14), S23 (= S15), E44 (= E36), A45 (≠ T37), W71 (= W63), A90 (≠ G83), G95 (= G88), C96 (≠ S89), H99 (≠ I92), N100 (= N93), S101 (≠ G94), I103 (≠ V96), R231 (≠ L220), A232 (≠ V221), T269 (≠ A255), G270 (= G256), D273 (≠ G259), V464 (≠ A468), Y465 (= Y469), H466 (= H470), D499 (= D501), A500 (≠ S502), N510 (= N512), P511 (≠ L513), N512 (= N514), V515 (≠ T517)
4mjwA Crystal structure of choline oxidase in complex with the reaction product glycine betaine (see paper)
33% identity, 94% coverage: 1:529/561 of query aligns to 9:527/532 of 4mjwA
- active site: I333 (= I323), P377 (≠ I383), N378 (≠ G384), V464 (≠ A468), H466 (= H470), V509 (≠ G511), N510 (= N512)
- binding flavin-adenine dinucleotide: G20 (= G12), G22 (= G14), S23 (= S15), E44 (= E36), A45 (≠ T37), W71 (= W63), R89 (= R82), A90 (≠ G83), G95 (= G88), C96 (≠ S89), H99 (≠ I92), N100 (= N93), S101 (≠ G94), I103 (≠ V96), R231 (≠ L220), A232 (≠ V221), T269 (≠ A255), G270 (= G256), D273 (≠ G259), Y465 (= Y469), H466 (= H470), A500 (≠ S502), N510 (= N512), P511 (≠ L513), N512 (= N514), V515 (≠ T517)
4ha6A Crystal structure of pyridoxine 4-oxidase - pyridoxamine complex (see paper)
33% identity, 92% coverage: 7:522/561 of query aligns to 3:498/508 of 4ha6A
- active site: F360 (≠ I383), G361 (= G384), H444 (≠ A468), H446 (= H470), G487 (= G511), P488 (≠ N512)
- binding flavin-adenine dinucleotide: G8 (= G12), G10 (= G14), S11 (= S15), A12 (= A16), E32 (= E36), A33 (≠ T37), W58 (= W63), R77 (= R82), G78 (= G83), G83 (= G88), S84 (= S89), L87 (≠ I92), H88 (≠ N93), A89 (≠ G94), M90 (= M95), G91 (≠ V96), V218 (= V221), A251 (= A255), G252 (= G256), E255 (≠ G259), H445 (≠ Y469), A478 (≠ S502), P488 (≠ N512), I489 (≠ L513), H490 (≠ N514)
- binding 4-(aminomethyl)-5-(hydroxymethyl)-2-methylpyridin-3-ol: A89 (≠ G94), S314 (≠ W318), H444 (≠ A468), H446 (= H470)
3t37A Crystal structure of pyridoxine 4-oxidase from mesorbium loti
33% identity, 92% coverage: 7:522/561 of query aligns to 3:498/509 of 3t37A
- active site: F360 (≠ I383), G361 (= G384), H444 (≠ A468), H446 (= H470), G487 (= G511), P488 (≠ N512)
- binding flavin-adenine dinucleotide: G8 (= G12), G10 (= G14), S11 (= S15), A12 (= A16), E32 (= E36), A33 (≠ T37), W58 (= W63), R77 (= R82), G78 (= G83), R79 (≠ K84), G83 (= G88), S84 (= S89), H88 (≠ N93), A89 (≠ G94), G91 (≠ V96), R217 (≠ L220), V218 (= V221), A251 (= A255), E255 (≠ G259), H445 (≠ Y469), A478 (≠ S502), P488 (≠ N512), I489 (≠ L513), H490 (≠ N514)
E4QP00 5-(hydroxymethyl)furfural oxidase; 5-hydroxymethylfurfural oxidase; HMFO; Thiol oxidase; EC 1.1.3.47; EC 1.8.3.- from Methylovorus sp. (strain MP688) (see paper)
32% identity, 94% coverage: 1:528/561 of query aligns to 1:527/531 of E4QP00
- V101 (≠ I92) mutation to H: Abolishes activity.
- M103 (≠ G94) mutation to A: 16-fold reduction in catalytic efficiency on vanillyl alcohol.
- V367 (≠ L381) mutation to K: 1.6-fold reduction in catalytic efficiency on vanillyl alcohol. Shows significantly improved activity on the aldehyde 5-formyl-2-furancarboxylate, which results in a better 5-hydroxymethylfurfural to 2,5-furandicarboxylate conversion.; mutation to R: 1.4-fold reduction in catalytic efficiency on vanillyl alcohol. Shows significantly improved activity on the aldehyde 5-formyl-2-furancarboxylate, which results in a better 5-hydroxymethylfurfural to 2,5-furandicarboxylate conversion. Displays a catalytic efficiency toward 5-formyl-2-furancarboxylate that is over 1000-fold higher than that for wild-type; when associated with F-466.
- W369 (≠ G384) mutation to A: 7.5-fold reduction in catalytic efficiency on vanillyl alcohol.
- V465 (≠ A468) mutation to A: 18-fold reduction in catalytic efficiency on vanillyl alcohol.
- W466 (≠ Y469) mutation to A: 39-fold reduction in catalytic efficiency on vanillyl alcohol. In contrast to wild-type, is active on secondary alcohols, such as (S)-1-phenylethanol, and is strictly enantionselective as this mutant has no activity on (R)-1-phenylethanol. Shows increased activity on the aldehyde 5-formyl-2-furancarboxylate.; mutation to F: 3.4-fold reduction in catalytic efficiency on vanillyl alcohol. In contrast to wild-type, is active on secondary alcohols, such as (S)-1-phenylethanol, and is strictly enantionselective as this mutant has no activity on (R)-1-phenylethanol. Shows increased activity on the aldehyde 5-formyl-2-furancarboxylate. Displays a catalytic efficiency toward 5-formyl-2-furancarboxylate that is over 1000-fold higher than that for wild-type; when associated with R-367.
- H467 (= H470) mutation to A: Abolishes activity.
- N511 (= N512) mutation to A: 53-fold reduction in catalytic efficiency on vanillyl alcohol.
4udqA Crystal structure of 5-hydroxymethylfurfural oxidase (hmfo) in the reduced state
32% identity, 93% coverage: 6:528/561 of query aligns to 2:523/525 of 4udqA
- active site: L331 (≠ C343), F364 (≠ I383), W365 (≠ G384), V461 (≠ A468), H463 (= H470), A506 (≠ G511), N507 (= N512)
- binding flavin-adenine dinucleotide: G8 (= G12), G10 (= G14), T11 (≠ S15), A12 (= A16), E32 (= E36), A33 (≠ T37), W64 (= W63), G88 (= G83), G93 (= G88), G94 (≠ S89), N98 (= N93), M99 (≠ G94), V101 (= V96), V229 (= V221), T261 (≠ S254), A262 (= A255), W462 (≠ Y469), H463 (= H470), A497 (≠ S502), N507 (= N512), T508 (≠ L513), N509 (= N514), T512 (= T517)
5oc1A Crystal structure of aryl-alcohol oxidase from pleurotus eryngii in complex with p-anisic acid (see paper)
31% identity, 94% coverage: 6:532/561 of query aligns to 2:565/565 of 5oc1A
- active site: V339 (≠ G313), N413 (≠ I383), A414 (≠ G384), I499 (≠ A468), H501 (= H470), A544 (≠ G511), H545 (≠ N512)
- binding 4-methoxybenzoic acid: Y91 (≠ G94), I356 (≠ R330), I390 (≠ L357), F396 (= F361), T412 (≠ H382), I499 (≠ A468), H501 (= H470), H545 (≠ N512)
- binding flavin-adenine dinucleotide: G8 (= G12), G10 (= G14), N11 (≠ S15), A12 (= A16), E32 (= E36), A33 (≠ T37), W60 (= W63), P78 (= P81), G80 (= G83), G85 (= G88), S86 (= S89), H90 (≠ N93), Y91 (≠ G94), V93 (= V96), V230 (= V221), S270 (= S254), A271 (= A255), G272 (= G256), F500 (≠ Y469), H545 (≠ N512), T546 (≠ L513), Q547 (≠ N514), I550 (≠ T517)
3fimB Crystal structure of aryl-alcohol-oxidase from pleurotus eryingii (see paper)
30% identity, 94% coverage: 6:532/561 of query aligns to 2:565/565 of 3fimB
- active site: V339 (≠ G313), N413 (≠ I383), A414 (≠ G384), I499 (≠ A468), H501 (= H470), A544 (≠ G511), H545 (≠ N512)
- binding flavin-adenine dinucleotide: G8 (= G12), N11 (≠ S15), A12 (= A16), E32 (= E36), A33 (≠ T37), W60 (= W63), P78 (= P81), G80 (= G83), G85 (= G88), S86 (= S89), H90 (≠ N93), Y91 (≠ G94), V93 (= V96), V230 (= V221), S270 (= S254), A271 (= A255), F500 (≠ Y469), H501 (= H470), H545 (≠ N512), T546 (≠ L513), Q547 (≠ N514), I550 (≠ T517)
8bxlB Patulin synthase from penicillium expansum
29% identity, 94% coverage: 6:530/561 of query aligns to 14:588/590 of 8bxlB
- binding flavin-adenine dinucleotide: G20 (= G12), G22 (= G14), T23 (≠ S15), A24 (= A16), E44 (= E36), A45 (≠ T37), W80 (vs. gap), G100 (= G83), G105 (= G88), S106 (= S89), R109 (≠ I92), N110 (= N93), Y111 (≠ G94), A113 (≠ V96), L253 (= L220), A254 (≠ V221), A288 (= A255), Q292 (≠ G259), F525 (≠ Y469), D559 (= D501), A560 (≠ S502), H570 (≠ N512), P571 (≠ L513), Q572 (≠ N514), L575 (≠ T517)
7vzsA Fad-dpendent glucose dehydrogenase complexed with an inhibitor at ph7.56
30% identity, 93% coverage: 6:529/561 of query aligns to 4:563/566 of 7vzsA
- binding D-glucal: Y6 (= Y8), L22 (= L24), N25 (≠ D27), Y51 (= Y61), I349 (≠ V325), Q356 (vs. gap), E411 (≠ H382), E444 (= E415), W445 (≠ A416), K448 (≠ E419), R499 (≠ E466), N501 (≠ A468), H546 (≠ N512), K563 (≠ R529)
- binding flavin-adenine dinucleotide: G10 (= G12), G12 (= G14), T13 (≠ S15), S14 (≠ A16), E34 (= E36), A35 (≠ T37), Y51 (= Y61), F55 (vs. gap), W61 (= W63), R79 (≠ P81), G81 (= G83), G86 (= G88), T87 (≠ S89), N91 (= N93), G92 (= G94), M93 (= M95), A94 (≠ V96), T232 (≠ L220), A233 (≠ V221), A273 (= A255), G274 (= G256), R277 (≠ G259), F502 (≠ Y469), A536 (≠ S502), H546 (≠ N512), L547 (= L513), V548 (≠ N514), L551 (≠ T517)
Sites not aligning to the query:
4yntA Crystal structure of aspergillus flavus fad glucose dehydrogenase (see paper)
30% identity, 93% coverage: 6:529/561 of query aligns to 5:564/570 of 4yntA
- active site: V342 (≠ W317), F413 (≠ I383), W414 (≠ G384), N502 (≠ A468), H504 (= H470), G546 (= G511), H547 (≠ N512)
- binding dihydroflavine-adenine dinucleotide: G13 (= G14), T14 (≠ S15), S15 (≠ A16), E35 (= E36), A36 (≠ T37), F56 (vs. gap), W62 (= W63), R80 (≠ P81), G82 (= G83), G87 (= G88), T88 (≠ S89), N92 (= N93), G93 (= G94), M94 (= M95), A95 (≠ V96), A234 (≠ V221), A274 (= A255), R278 (≠ G259), F503 (≠ Y469), A537 (≠ S502), H547 (≠ N512), L548 (= L513), V549 (≠ N514), L552 (≠ T517)
4ynuA Crystal structure of aspergillus flavus fadgdh in complex with d- glucono-1,5-lactone (see paper)
30% identity, 93% coverage: 6:529/561 of query aligns to 4:563/569 of 4ynuA
- active site: V341 (≠ W317), F412 (≠ I383), W413 (≠ G384), N501 (≠ A468), H503 (= H470), G545 (= G511), H546 (≠ N512)
- binding flavin-adenine dinucleotide: G12 (= G14), T13 (≠ S15), S14 (≠ A16), E34 (= E36), A35 (≠ T37), Y51 (= Y61), F55 (vs. gap), W61 (= W63), R79 (≠ P81), G81 (= G83), G86 (= G88), T87 (≠ S89), N91 (= N93), G92 (= G94), T232 (≠ L220), A233 (≠ V221), A273 (= A255), G274 (= G256), R277 (≠ G259), F502 (≠ Y469), A536 (≠ S502), H546 (≠ N512), L547 (= L513), V548 (≠ N514), L551 (≠ T517)
- binding D-glucono-1,5-lactone: Y51 (= Y61), E411 (≠ H382), A496 (≠ Q463), N497 (≠ A464), R499 (≠ E466), R499 (≠ E466), N501 (≠ A468), H503 (= H470), H546 (≠ N512)
4h7uA Crystal structure of pyranose dehydrogenase from agaricus meleagris, wildtype (see paper)
28% identity, 93% coverage: 6:528/561 of query aligns to 16:572/577 of 4h7uA
- active site: A343 (≠ G313), V426 (≠ G384), Y510 (≠ A468), H512 (= H470), A555 (≠ G511), H556 (≠ N512)
- binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl (2R,3S,4S)-2,3,4-trihydroxy-5-[(4aR)-4a-hydroxy-7,8-dimethyl-2,4-dioxo-3,4,4a,5-tetrahydrobenzo[g]pteridin-10(2H)-yl]pentyl dihydrogen diphosphate (non-preferred name): G22 (= G12), G24 (= G14), T25 (≠ S15), A26 (= A16), E46 (= E36), A47 (≠ T37), W74 (= W63), G99 (= G88), C100 (≠ S89), H103 (≠ I92), N104 (= N93), G105 (= G94), V107 (= V96), L242 (= L220), V243 (= V221), G282 (≠ A255), G283 (= G256), A286 (≠ G259), H512 (= H470), A546 (≠ S502), H556 (≠ N512), T557 (≠ L513), Q558 (≠ N514), V561 (≠ T517)
Q3L245 Pyranose dehydrogenase 1; PDH 1; Pyranose:quinone oxidoreductase 1; EC 1.1.99.29 from Leucoagaricus meleagris (Western flat-topped agaric) (Agaricus meleagris) (see 2 papers)
28% identity, 93% coverage: 6:528/561 of query aligns to 41:597/602 of Q3L245
- N100 (≠ Q64) modified: carbohydrate, N-linked (GlcNAc...) asparagine
- H128 (≠ I92) modified: Tele-8alpha-FAD histidine
- N344 (= N292) modified: carbohydrate, N-linked (GlcNAc...) asparagine
- H537 (= H470) active site, Proton acceptor
- H581 (≠ N512) active site
Sites not aligning to the query:
6h3oF Alcohol oxidase from phanerochaete chrysosporium mutant f101s (see paper)
28% identity, 93% coverage: 7:528/561 of query aligns to 7:619/650 of 6h3oF
- binding flavin-adenine dinucleotide: P15 (≠ S15), E37 (= E36), G38 (≠ T37), N90 (≠ K84), I91 (≠ V85), G94 (= G88), G95 (≠ S89), N99 (= N93), S100 (≠ G94), Q101 (≠ M95), M102 (≠ V96), R227 (≠ L220), V228 (= V221), S271 (≠ A255), I279 (≠ L263), W559 (≠ Y469), L593 (≠ S502), N603 (= N512), T604 (≠ L513), Y605 (≠ N514), A608 (≠ T517)
Query Sequence
>GFF3683 HP15_3625 choline dehydrogenase
MTENRYDYIIVGAGSAGCVLANRLTEDARHRVLLLETGGSDKSIFIQMPTALSIPMNTKK
YAWQFETEPEPFLDNRRMHCPRGKVLGGSSSINGMVYVRGHARDFDEWDSEGATGWHYRN
VLPYFRKAETWAFGGNDYRGDKGPLGVNNGNNMQNPLYKAFIRAGSDAGYFETDDYNGTQ
QEGFGAMHMTVKNGRRWSTANAYLRPAMDRDNLTVVTHALVHKVLLDGKTATGVRYEQGG
KVHEAKAAEEVILSAGSIGSPHLLQLSGIGKREVLEKAGIEVKHELPGVGENLQDHLEFY
FQYRCKQPVSLNGKLDWWNKLKIGVRWILRKDGLGATNHFESCGFIRSKAGVEWPDLQYH
FLPAAMRYDGKEAFNGDGFQLHIGHNKPKSRGFVHVQSADPKQAPTIRFNYLQHEADREG
FRDCVRLTREIINQPAMDEYRGAEIQPGAEVQTDEEIDAFVRQAVESAYHPSCSCKMGTD
ELAVVDPETRVHGIRNLRVVDSSIFPTIPNGNLNSPTIMVAERAADLIRGREPLQPSDAP
VVMDDQWQTRQRPGQAKRALA
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory