SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF3721 FitnessBrowser__WCS417:GFF3721 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

O59010 Glutamate transporter homolog; Glt(Ph); Sodium-aspartate symporter Glt(Ph); Sodium-dependent aspartate transporter from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see 3 papers)
34% identity, 96% coverage: 8:410/420 of query aligns to 14:420/425 of O59010

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O59010

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S65 (≠ T63) mutation to V: Strongly decreased chloride conductance.
R276 (≠ A272) mutation to S: Increased rate of aspartate transport; when associated with R-395.
RSS 276:278 (≠ ASS 272:274) binding
M311 (≠ L307) mutation to A: Decreased dependence of aspartate binding on Na(+) concentration.
T314 (≠ S310) binding
V355 (= V351) binding
D394 (= D384) binding
M395 (= M385) mutation to R: Increased rate of aspartate transport; when associated with S-276.
R397 (= R387) mutation to A: Strongly decreased affinity for aspartate.
N401 (= N391) binding
D405 (≠ N395) mutation to N: Strongly decreased affinity for aspartate.

2nwwA Crystal structure of gltph in complex with tboa (see paper)
34% identity, 95% coverage: 8:406/420 of query aligns to 5:407/407 of 2nwwA

query
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binding (3s)-3-(benzyloxy)-l-aspartic acid: R267 (≠ A272), S268 (= S273), S269 (= S274), T305 (≠ S310), G348 (≠ R353), A349 (= A354), G350 (≠ S355), D385 (= D384), R388 (= R387), T389 (= T388), N392 (= N391)

6bauA Crystal structure of gltph r397c in complex with l-cysteine (see paper)
33% identity, 95% coverage: 8:406/420 of query aligns to 6:408/408 of 6bauA

query
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E

E

binding cysteine: R268 (≠ A272), S270 (= S274), M303 (≠ L307), T306 (≠ S310), A345 (= A349), G346 (= G350), V347 (= V351), P348 (≠ A352), G351 (≠ S355), D386 (= D384), C389 (≠ R387), T390 (= T388), N393 (= N391)

6bavA Crystal structure of gltph r397c in complex with s-benzyl-l-cysteine (see paper)
33% identity, 95% coverage: 8:406/420 of query aligns to 6:408/409 of 6bavA

query
sites
6bavA

R

Q

R

K

I

I

A

L

V

I

G

G

I

L

A

I

L

L

G

G

V

A

L

I

V

V

G

G

W

L

A

I

C

L

H

G

H

H

Y

Y

A

G

G

-

S

-

E

-

Q

-

A

Y

A

A

K

D

A

A

L

V

A

K

G

T

Y

Y

F

V

S

K

M

P

V

F

T

G

D

D

I

L

F

F

L

V

R

R

M

L

I

L

K

K

M

M

I

L

I

V

A

M

P

P

L

I

V

V

F

F

A

A

T

S

L

L

V

V

G

V

G

G

I

A

A

A

S

S

M

I

G

S

N

P

S

A

R

R

S

-

V

L

G

G

R

R

I

V

G

G

M

V

R

K

A

I

M

V

L

V

W

Y

F

Y

V

L

T

L

A

T

S

S

L

A

V

F

S

A

L

V

M

T

L

L

G

G

M

I

A

I

L

M

V

A

N

R

L

L

F

F

Q

N

P

P

G

G

A

A

G

G

L

I

N

H

M

L

Q

A

V

V

-

G

V

Q

Q

Q

H

F

A

Q

T

P

A

K

A

Q

V

A

P

P

V

-

N

-

T

-

G

-

D

-

F

-

S

P

L

L

K

V

T

K

F

I

I

L

S

L

H

D

V

I

F

V

P

P

R

T

S

N

I

P

A

F

E

G

A

A

M

L

A

A

N

N

N

G

E

Q

I

V

L

L

Q

P

I

T

V

I

V

F

F

F

S

A

L

I

F

I

F

L

G

G

F

I

A

A

L

I

A

T

-

Y

-

L

-

M

-

N

-

S

-

E

-

N

-

E

G

K

V

V

K

R

R

K

A

S

G

A

Y

E

T

T

R

L

I

L

T

-

D

D

T

A

V

I

D

N

E

G

L

L

A

A

K

E

V

A

M

M

F

Y

K

K

I

I

T

V

D

N

Y

G

V

V

M

M

A

Q

F

Y

A

A

P

P

I

I

G

G

V

V

F

F

A

A

A

L

I

I

A

A

S

Y

A

V

I

M

T

A

T

E

N

Q

G

G

L

V

G

K

L

V

A

G

D

E

Y

L

A

A

K

K

L

V

I

T

A

A

E

A

F

V

Y

Y

L

V

G

G

I

L

A

T

L

L

L

Q

W

I

V

L

L

L

L

V

F

Y

A

F

A

V

G

L

Y

L

L

K

F

I

L

Y

G

G

R

I

S

D

V

P

F

I

T

S

L

F

G

I

K

K

L

K

I

A

R

K

E

D

P

A

I

M

L

L

L

T

A

A

F

F

S

V

T

T

A
x
R

S
|
S

E

S

S

G

A

T

Y

L

P

P

K

V

T

T

M

M

E

R

A

V

L

A

E

K

K

E

F

M

G

G

A

I

P

S

K

E

R

G

V

I

S

Y

S

S

F

F

V

T

L

L

P

P

L

L

G

G

Y

A

S

T

F

I

N

N

L
x
M

D

D

G

G

S
x
T

M

A

M

L

Y
|
Y

Q

Q

A

G

F

V

A

C

I

T

M

F

F

F

I

I

A

A

Q

N

A

A

Y

L

N

G

I

S

D

H

L

L

S

T

F

V

T

G

Q

Q

L

L

L

T

I

I

L

V

L

L

T

T

L

A

M

V

I

L

T

A

S

S

K

I

G

G

M

T

A

A

G

G

V

V

A

P

R

G

A

A

S

G

V

A

V

I

V

M

V

L

A

A

A

M

T

V

L

L

P

E

M

S

F

V

N

G

L

L

P

P

-

L

-

T

-

D

-

P

-

N

-

V

E

A

A

A

G

A

L

Y

L

A

L

M

I

I

I

L

G

G

I

I

D
|
D

Q

A

F

I

L

L

D
|
D

M

M

A

G

R
x
C

T
|
T

A

M

T

V

N
|
N

V

V

V

T

G

G

N

D

S

L

I

T

A

G

T

T

A

A

V

I

V

V

A

A

K

K

S

T

E

E

binding benzylcysteine: R268 (≠ A272), S269 (= S273), S270 (= S274), M303 (≠ L307), T306 (≠ S310), Y309 (= Y313), D382 (= D380), D386 (= D384), C389 (≠ R387), T390 (= T388), N393 (= N391)

6x14A Inward-facing state of the glutamate transporter homologue gltph in complex with tfb-tboa (see paper)
33% identity, 95% coverage: 8:406/420 of query aligns to 11:413/413 of 6x14A

query
sites
6x14A

R

Q

R

K

I
|
I

A

L

V

I

G

G

I

L

A

I

L

L

G

G

V

A

L

I

V

V

G

G

W

L

A

I

C

L

H

G

H

H

Y

Y

A

G

G

-

S

-

E

-

Q

-

A

Y

A

A

K

H

A

A

L

V

A

H

G

T

Y

Y

F

V

S

K

M

P

V

F

T

G

D

D

I

L

F
|
F

L

V

R

R

M

L

I

L

K

C

M

M

I

L

I

V

A

M

P

P

L

I

V

V

F

F

A

A

T

S

L

L

V

V

G

V

G
|
G

I
x
A

A

A

S

S

M
x
I

G

S

N

P

S

A

R

R

S

-

V

L

G

G

R

R

I

V

G

G

M

V

R

K

A

I

M
x
V

L

V

W

Y

F

Y

V

L

T

L

A

T

S

S

L

A

V

F

S

A

L

V

M

T

L

L

G

G

M

I

A

I

L

M

V

A

N

R

L

L

F

F

Q

N

P

P

G

G

A

A

G

G

L

I

N

H

M

L

Q

A

V

V

-

G

V

Q

Q

Q

H

F

A

Q

T

P

A

H

A

Q

V

A

P

P

V

-

N

-

T

-

G

-

D

-

F

-

S

P

L

L

K

V

T

H

F

I

I

L

S

L

H

D

V

I

F

V

P

P

R

T

S

N

I

P

A

F

E

G

A

A

M

L

A

A

N

N

N

G

E

Q

I

V

L

L

Q

P

I

T

V

I

V

F

F

F

S

A

L

I

F
x
I

F

L

G

G

F

I

A

A

L

I

A

T

-
x
Y

-

L

-

M

-

N

-

S

-

E

-

N

-

E

G

K

V

V

K

R

R

K

A

S

G

A

Y

E

T

T

R

-

I

L

T

L

D

D

T

A

V

I

D

N

E

G

L

L

A

A

K

E

V

A

M

M

F

Y

K
|
K

I
|
I

T

V

D
x
N

Y
x
G

V

V

M
|
M

A
x
Q

F
x
Y

A

A

P

P

I

I

G

G

V

V

F

F

A

A

A

L

I

I

A

A

S

Y

A

V

I

M

T

A

T

E

N

Q

G

G

L

V

G

H

L

V

A

G

D

E

Y

L

A

A

K

K

L

V

I

T

A

A

E

A

F

V

Y

Y

L

V

G

G

I

L

A

T

L

L

L

Q

W

I

V

L

L

L

L

V

F

Y

A

F

A

V

G

L

Y

L

L

K

F

I

L

Y

G

G

R

I

S

D

V

P

F

I

T

S

L

F

G

I

K

K

L

H

I

A

R

K

E

D

P

A

I

M

L

L

L

T

A

A

F

F

S

V

T

T

A
x
R

S
|
S

E

S

S

G

A

T

Y

L

P

P

K

V

T

T

M

M

E

R

A

V

L

A

E

K

K

E

F

M

G

G

A

I

P

S

K

E

R

G

V

I

S

Y

S

S

F

F

V

T

L

L

P

P

L
|
L

G

G

Y

A

S
x
T

F
x
I

N

N

L
x
M

D

D

G

G

S
x
T

M

A

M

L

Y

Y

Q

Q

A

G

F

V

A

A

I

T

M

F

F

F

I

I

A

A

Q

N

A

A

Y

L

N

G

I

S

D

H

L

L

S

T

F

V

T

G

Q

Q

L

L

L

T

I

I

L

V

L

L

T

T

L

A

M

V

I

L

T

A

S

S

K
x
I

G

G

M

T

A
|
A

G

G

V

V

A
x
P

R

G

A

A

S
x
G

V
x
A

V

I

V

M

V

L

A

C

A

M

T

V

L

L

P

H

M

S

F

V

N

G

L

L

P

P

-

L

-

T

-

D

-

P

-

N

-

V

E

A

A

A

G

A

L

Y

L

A

L

M

I

I

I
x
L

G

G

I

I

D

D

Q

A

F

I

L

L

D
|
D

M

M

A

G

R
|
R

T
|
T

A

M

T

V

N

N

V

V

V

T

G

G

N

D

S

L

I

T

A

G

T

T

A

A

V

I

V

V

A

A

K

K

S

T

E

E

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: G66 (= G67), A67 (≠ I68), I70 (≠ M71), V83 (≠ M85), I157 (≠ F163), Y164 (vs. gap), K193 (= K192), I194 (= I193), N196 (≠ D195), G197 (≠ Y196), Q200 (≠ A199), Y201 (≠ F200), L302 (= L301), T305 (≠ S304), I306 (≠ F305), I347 (≠ K346), A350 (= A349)
binding (2~{S},3~{S})-2-azanyl-3-[[3-[[4-(trifluoromethyl)phenyl]carbonylamino]phenyl]methoxy]butanedioic acid: I13 (= I10), F47 (= F48), M199 (= M198), R273 (≠ A272), S274 (= S273), S275 (= S274), M308 (≠ L307), T311 (≠ S310), P353 (≠ A352), G356 (≠ S355), A357 (≠ V356), L384 (≠ I377), D391 (= D384), R394 (= R387), T395 (= T388)

Sites not aligning to the query:

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: 1, 4

6x15A Inward-facing state of the glutamate transporter homologue gltph in complex with l-aspartate and sodium ions (see paper)
33% identity, 95% coverage: 8:406/420 of query aligns to 14:416/419 of 6x15A

query
sites
6x15A

R

Q

R
x
K

I

I

A

L

V
x
I

G

G

I

L

A

I

L

L

G

G

V

A

L

I

V

V

G

G

W

L

A

I

C

L

H

G

H

H

Y

Y

A

G

G

-

S

-

E

-

Q

-

A

Y

A

A

K

H

A

A

L

V

A

H

G

T

Y
|
Y

F

V

S

K

M
x
P

V
x
F

T

G

D

D

I
x
L

F

F

L

V

R

R

M
x
L

I

L

K

C

M

M

I

L

I

V

A

M

P

P

L

I

V

V

F

F

A

A

T

S

L

L

V

V

G

V

G

G

I

A

A

A

S

S

M
x
I

G

S

N
x
P

S

A

R

R

S

-

V

L

G

G

R

R

I

V

G

G

M

V

R
x
K

A

I

M

V

L

V

W

Y

F
x
Y

V
x
L

T
x
L

A
x
T

S
|
S

L

A

V
x
F

S

A

L

V

M

T

L

L

G

G

M

I

A

I

L

M

V

A

N

R

L

L

F

F

Q

N

P

P

G

G

A

A

G

G

L

I

N

H

M

L

Q

A

V

V

-

G

V

Q

Q

Q

H

F

A

Q

T

P

A

H

A

Q

V

A

P

P

V

-

N

-

T

-

G

-

D

-

F

-

S

P

L
|
L

K
x
V

T

H

F
x
I

I
x
L

S

L

H

D

V
x
I

F

V

P

P

R

T

S

N

I

P

A

F

E

G

A

A

M

L

A

A

N

N

N

G

E

Q

I

V

L
|
L

Q

P

I

T

V

I

V
x
F

F

F

S

A

L
x
I

F

I

F

L

G

G

F
x
I

A
|
A

L

I

A

T

-
x
Y

-

L

-

M

-

N

-

S

-

E

-

N

-

E

G

K

V

V

K

R

R

K

A

S

G

A

Y

E

T

T

R

-

I

L

T

L

D

D

T

A

V

I

D

N

E

G

L

L

A

A

K

E

V

A

M

M

F

Y

K
|
K

I
|
I

T

V

D
x
N

Y
x
G

V

V

M

M

A

Q

F
x
Y

A

A

P

P

I
|
I

G

G

V

V

F
|
F

A

A

A

L

I

I

A

A

S

Y

A

V

I

M

T

A

T

E

N

Q

G

G

L

V

G

H

L

V

A

G

D

E

Y

L

A

A

K

K

L

V

I

T

A

A

E

A

F

V

Y

Y

L

V

G

G

I

L

A

T

L

L

L

Q

W

I

V

L

L

L

L

V

F

Y

A

F

A

V

G
x
L

Y

L

L

K

F
x
I

L
x
Y

G

G

R

I

S

D

V

P

F

I

T

S

L

F

G
x
I

K

K

L

H

I

A

R
x
K

E

D

P

A

I
x
M

L

L

L

T

A

A

F

F

S

V

T

T

A
x
R

S
|
S

E

S

S

G

A

T

Y

L

P

P

K

V

T

T

M

M

E

R

A

V

L

A

E

K

K

E

F

M

G

G

A

I

P

S

K

E

R

G

V

I

S

Y

S

S

F

F

V

T

L

L

P

P

L

L

G
|
G

Y
x
A

S
x
T

F

I

N
|
N

L
x
M

D
|
D

G

G

S
x
T

M

A

M

L

Y

Y

Q

Q

A

G

F

V

A

A

I

T

M

F

F

F

I

I

A

A

Q

N

A

A

Y

L

N

G

I

S

D

H

L

L

S
x
T

F
x
V

T
x
G

Q
|
Q

Q

Q

L
|
L

L
x
T

I

I

L

V

L
|
L

T

T

L

A

M

V

I

L

T

A

S
|
S

K
x
I

G
|
G

M
x
T

A

A

G
|
G

V
|
V

A

P

R
x
G

A
|
A

S
x
G

V

A

V

I

V

M

V

L

A

C

A

M

T

V

L

L

P

H

M
x
S

F

V

N

G

L

L

P

P

-

L

-

T

-

D

-

P

-

N

-

V

E

A

A

A

G

A

L

Y

L

A

L

M

I

I

I

L

G

G

I

I

D

D

Q

A

F

I

L

L

D
|
D

M

M

A

G

R
|
R

T
|
T

A
x
M

T

V

N
|
N

V
|
V

V

T

G

G

N
x
D

S

L

I

T

A

G

T

T

A

A

V

I

V

V

A

A

K

K

S

T

E

E

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: K15 (≠ R9), I18 (≠ V12), Y42 (= Y40), P45 (≠ M43), F46 (≠ V44), F46 (≠ V44), F46 (≠ V44), L49 (≠ I47), L49 (≠ I47), L53 (≠ M51), I73 (≠ M71), P75 (≠ N73), K84 (≠ R83), L90 (≠ V89), L91 (≠ T90), L91 (≠ T90), F95 (≠ V94), L130 (= L133), V131 (≠ K134), I133 (≠ F136), L134 (≠ I137), I137 (≠ V140), L152 (= L155), F156 (≠ V159), I159 (≠ L162), I163 (≠ F166), I163 (≠ F166), I163 (≠ F166), A164 (= A167), Y167 (vs. gap), Y167 (vs. gap), K196 (= K192), I197 (= I193), N199 (≠ D195), G200 (≠ Y196), Y204 (≠ F200), I207 (= I203), F210 (= F206), L250 (≠ G246), I253 (≠ F249), Y254 (≠ L250), Y254 (≠ L250), I262 (≠ G258), K266 (≠ R262), M269 (≠ I265), T334 (≠ S330), V335 (≠ F331), G336 (≠ T332), Q337 (= Q333), L339 (= L335), T340 (≠ L336), L343 (= L339), S369 (≠ M365), M399 (≠ A389)
binding aspartic acid: R276 (≠ A272), S277 (= S273), S278 (= S274), T314 (≠ S310), T352 (≠ M348), G354 (= G350), V355 (= V351), G357 (≠ R353), A358 (= A354), G359 (≠ S355), D394 (= D384), R397 (= R387), T398 (= T388), N401 (= N391)
binding sodium ion: Y89 (≠ F88), T92 (≠ A91), S93 (= S92), G306 (= G302), A307 (≠ Y303), T308 (≠ S304), N310 (= N306), N310 (= N306), M311 (≠ L307), D312 (= D308), S349 (= S345), I350 (≠ K346), G351 (= G347), T352 (≠ M348), N401 (= N391), V402 (= V392), D405 (≠ N395)

Sites not aligning to the query:

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: 1, 3, 4, 4, 6, 7, 8

6bmiA Crystal structure of gltph r397c in complex with l-serine (see paper)
32% identity, 95% coverage: 8:406/420 of query aligns to 6:396/396 of 6bmiA

query
sites
6bmiA

R

Q

R

K

I

I

A

L

V

I

G

G

I

L

A

I

L

L

G

G

V

A

L

I

V

V

G

G

W

L

A

I

C

L

H

G

H

H

Y

Y

A

G

G

-

S

-

E

-

Q

-

A

Y

A

A

K

D

A

A

L

V

A

K

G

T

Y

Y

F

V

S

K

M

P

V

F

T

G

D

D

I

L

F

F

L

V

R

R

M

L

I

L

K

K

M

M

I

L

I

V

A

M

P

P

L

I

V

V

F

F

A

A

T

S

L

L

V

V

G

V

G

G

I

A

A

A

S

S

M

I

G

S

N

P

S

A

R

R

S

-

V

L

G

G

R

R

I

V

G

G

M

V

R

K

A

I

M

V

L

V

W

Y

F

Y

V

L

T

L

A

T

S

S

L

A

V

F

S

A

L

V

M

T

L

L

G

G

M

I

A

I

L

M

V

A

N

R

L

L

F

F

Q

N

P

P

G

G

A

A

G

G

L

I

N

H

M

L

Q

A

V

V

-

G

V

Q

Q

Q

H

F

A

Q

T

P

A

K

A

Q

V

A

P

P

V

-

N

-

T

-

G

-

D

-

F

-

S

P

L

L

K

V

T

K

F

I

I

L

S

L

H

D

V

I

F

V

P

P

R

T

S

N

I

P

A

F

E

G

A

A

M

L

A

A

N

N

N

G

E

Q

I

V

L

L

Q

P

I

T

V

I

V

F

F

F

S

A

L

I

F

I

F

L

G

G

F

I

A

A

L

I

A

T

-

Y

-

L

-

M

-

N

-

S

-

E

-

N

-

E

G

K

V

V

K

R

R

K

A

S

G

A

Y

E

T

T

R

-

I

L

T

L

D

D

T

A

V

I

D

N

E

G

L

L

A

A

K

E

V

A

M

M

F

Y

K

K

I

I

T

V

D

N

Y

G

V

V

M

M

A

Q

F

Y

A

A

P

P

I

I

G

G

V

V

F

F

A

A

A

L

I

I

A

A

S

Y

A

V

I

M

T

A

T

E

N

Q

G

G

L

V

G

K

L

V

A

G

D

E

Y

L

A

A

K

K

L

V

I

T

A

A

E

A

F

V

Y

Y

L

V

G

G

I

L

A

T

L

L

L

Q

W

I

V

L

L

L

L

V

F

Y

A

F

A

V

G

L

Y

L

L

K

F

I

L

Y

G

G

R

I

S

D

V

P

F

I

T

S

L

F

G

I

K

K

L

K

I

A

R

K

E

D

P

A

I

M

L

L

L

T

A

A

F

F

S

V

T

T

A
x
R

S
|
S

E

S

S

G

A

T

Y

L

P

P

K

V

T

T

M

M

E

R

A

V

L

A

E

K

K

E

F

M

G

G

A

I

P

S

K

E

R

G

V

I

S

Y

S

S

F

F

V

T

L

L

P

P

L

L

G

G

Y

A

S

T

F

I

N

N

L
x
M

D

D

G

G

S
x
T

M

A

M

L

Y

Y

Q

Q

A

G

F

V

A

C

I

T

M

F

F

F

I

I

A

A

Q

N

A

A

Y

L

N

G

I

S

D

H

L

L

S

T

F

V

T

G

Q

Q

L

L

L

T

I

I

L

V

L

L

T

T

L

A

M

V

I

L

T

A

S

-

K

-

G

-

M

-

A

-

G

-

V

-

A

I

R

M

A

L

S

A

V

M

V

V

V

L

V

E

A

S

A

V

T

G

L

L

P

P

M

L

F

T

N

D

L

-

P

P

E

N

-

V

-

A

A

A

G

A

L

Y

L

A

L

M

I

I

I

L

G

G

I

I

D

D

Q

A

F

I

L

L

D
|
D

M

M

A

G

R

C

T
|
T

A

M

T

V

N
|
N

V

V

V

T

G

G

N

D

S

L

I

T

A

G

T

T

A

A

V

I

V

V

A

A

K

K

S

T

E

E

binding serine: R268 (≠ A272), S269 (= S273), S270 (= S274), M303 (≠ L307), T306 (≠ S310), D374 (= D384), T378 (= T388), N381 (= N391)

6r7rA Crystal structure of the glutamate transporter homologue glttk in complex with d-aspartate (see paper)
32% identity, 96% coverage: 9:413/420 of query aligns to 6:412/416 of 6r7rA

query
sites
6r7rA

R

K

I

I

A

L

V

W

G

G

I

L

A

V

L

L

G

G

V

A

L

V

V

F

G

G

W

L

A

I

C

A

H

G

H
|
H

Y

F

A

G

G

-

S

-

E

-

Q

-

A

Y

A

A

K

G

A

A

L

V

A

K

G

T

Y

Y

F

I

S

K

M

P

V

F

T

G

D

D

I

L

F

F

L

V

R

R

M

L

I

L

K

K

M

M

I

L

I

V

A

M

P

P

L

I

V

V

F

L

A

A

T

S

L

L

V

V

G

V

G

G

I

A

A

A

S

S

M

I

G

S

N

P

S

A

R

R

S

-

V

L

G

G

R

R

I

V

G

G

M

V

R

K

A

I

M

V

L

V

W

Y

F

Y

V

L

T

A

A

T

S

S

L

A

V

M

S

A

L

V

M

F

L

F

G

G

M

L

A

I

L

V

V

G

N

R

L

L

F

F

Q

N

P

V

G

G

A

A

G

N

L

V

N

N

M

L

Q

G

V

-

Q

-

H

S

A

G

T

T

A

G

A

A

V

Q

P

P

V

P

N

-

T

-

G

-

D

-

F

-

S

S

L

L

K

V

T

Q

F

T

I

L

S

L

H

N

V

I

F

V

P

P

R

T

S

N

I

P

A

F

E

A

A

S

M

L

A

A

N

K

N

G

E

E

I

V

L

L

Q

P

I

V

V

I

V

F

F

F

S

A

L

I

F

I

F

L

G

G

F

I

A

A

L

I

A

T

G

Y

V

L

K

M

R

N

A

R

G

N

Y

E

T

E

R

R

I

V

T

R

D

K

T

S

V

A

-

E

-

T

-

L

-

R

-

V

-

F

D

D

E

G

L

L

A

A

K

E

V

A

M

M

F

Y

K

L

I

I

T

V

D

G

Y

G

V

V

M

M

A

Q

F

Y

A

A

P

P

I

I

G

G

V

V

F

F

A

A

A

L

I

I

A

A

S

Y

A

V

I

M

T

A

T

E

N

Q

G

G

L

V

G

R

L
x
V

A

G

D

P

Y

L

A
|
A

K
|
K

L

V

I

V

A
x
G

E

A

F

V

Y

Y

L

T

G

G

I

L

A

F

L

L

L

Q

W

I

V

V

L

I

L

T

F

Y

A

-

G

-

Y

-

L

-

F

F

L

I

G

L

R

L

S

K

V

V

F

F

T

G

L

I

G

D

-

P

-

I

K

K

L

F

I

I

R

R

-

K

-

A

-

K

E

D

P

A

I

M

L

I

L

T

A

A

F

F

S

V

T

T

A
x
R

S
|
S

E

S

S

G

A

T

Y

L

P

P

K

V

T

T

M

M

E

R

-

V

A

A

L

E

E

E

K

E

F

M

G

G

A

V

P

D

K

K

R

G

V

I

S

F

S

S

F

F

V

T

L

L

P

P

L

L

G

G

Y

A

S

T

F

I

N

N

L
x
M

D

D

G

G

S
x
T

M

A

M

L

Y

Y

Q

Q

A

G

F

V

A

T

I

V

M

L

F

F

I

V

A

A

Q

N

A

A

Y

I

N

G

I

H

D

P

L

L

S

T

F

L

T

G

Q

Q

L

L

L

V

I

V

L

V

L

L

T

T

L

A

M

V

I

L

T

A

S

S

K

I

G

G

M
x
T

A
|
A

G
|
G

V
|
V

A

P

R

G

A

A

S
x
G

V

A

V

I

V

M

V

L

A

A

A

M

T

V

L

L

P

Q

M

S

F

V

N

G

L

L

-

D

-

L

-

T

P

P

E

G

A

S

G

P

L

V

L

A

L

L

-

A

-

Y

-

A

-

M

I

I

I

L

G

G

I

I

D

D

Q

A

F

I

L

L

D
|
D

M

M

A

G

R
|
R

T
|
T

A

M

T

V

N
|
N

V

V

V

T

G

G

N

D

S

L

I

A

A

G

T

T

A

V

V

I

V

V

A

A

K

K

S

T

E

E

A

K

E

E

E

L

E

D

P

E

A

S

E

K

binding d-aspartic acid: R263 (≠ A272), S264 (= S273), S265 (= S274), M299 (≠ L307), T302 (≠ S310), T340 (≠ M348), A341 (= A349), G342 (= G350), V343 (= V351), G347 (≠ S355), D383 (= D384), R386 (= R387), T387 (= T388), N390 (= N391)
binding decyl-beta-d-maltopyranoside: H23 (= H26), V211 (≠ L220), V212 (≠ L221), A216 (= A225), K217 (= K226), G220 (≠ A229)

6zl4A The structure of glutamate transporter homologue glttk in complex with the photo switchable compound (cis) (see paper)
32% identity, 96% coverage: 9:413/420 of query aligns to 10:420/424 of 6zl4A

query
sites
6zl4A

R

K

I

I

A

L

V

W

G

G

I

L

A

V

L

L

G

G

V

A

L

V

V

F

G

G

W

L

A

I

C

A

H

G

H

H

Y

F

A

G

G

-

S

-

E

-

Q

-

A

Y

A

A

K

G

A

A

L

V

A

K

G

T

Y

Y

F

I

S

K

M

P

V

F

T

G

D

D

I

L

F

F

L

V

R

R

M

L

I

L

K

K

M

M

I

L

I

V

A

M

P

P

L

I

V

V

F

L

A

A

T

S

L

L

V

V

G

V

G

G

I

A

A

A

S

S

M

I

G

S

N

P

S

A

R

R

S

-

V

L

G

G

R

R

I

V

G

G

M

V

R

K

A

I

M

V

L

V

W

Y

F

Y

V

L

T

A

A

T

S

S

L

A

V

M

S

A

L

V

M

F

L

F

G

G

M

L

A

I

L

V

V

G

N

R

L

L

F

F

Q

N

P

V

G

G

A

A

G

N

L

V

N

N

M

L

-

G

Q

S

V

G

V

T

Q

G

H

K

A

A

T

I

A

E

A

A

V

Q

P

P

V

P

N

-

T

-

G

-

D

-

F

-

S

S

L

L

K

V

T

Q

F

T

I

L

S

L

H

N

V

I

F

V

P

P

R

T

S

N

I

P

A

F

E

A

A

S

M

L

A

A

N

K

N

G

E

E

I

V

L

L

Q

P

I

V

V

I

V

F

F

F

S

A

L

I

F

I

F

L

G

G

F

I

A

A

L

I

A

T

G

Y

V

L

K

M

R

N

A

R

G

N

Y

E

T

E

R

R

I

V

T

R

D

K

T

S

V

A

-

E

-

T

-

L

-

R

-

V

-

F

D

D

E

G

L

L

A

A

K

E

V

A

M

M

F
x
Y

K
x
L

I

I

T

V

D
x
G

Y
x
G

V

V

M

M

A
x
Q

F

Y

A

A

P

P

I

I

G

G

V

V

F

F

A

A

A

L

I

I

A

A

S

Y

A

V

I

M

T

A

T

E

N

Q

G

G

L

V

G

R

L

V

A

G

D

P

Y

L

A

A

K

K

L

V

I

V

A

G

E

A

F

V

Y

Y

L

T

G

G

I

L

A

F

L

L

L

Q

W

I

V

V

L

I

L

T

F

Y

A

-

G

-

Y

-

L

-

F

F

L

I

G

L

R

L

S

K

V

V

F

F

T

G

L

I

G

D

-

P

-

I

K

K

L

F

I

I

R

R

-

K

-

A

-

K

E

D

P

A

I

M

L

I

L

T

A

A

F

F

S

V

T

T

A
x
R

S
|
S

E

S

S

G

A

T

Y

L

P

P

K

V

T

T

M

M

E
x
R

-

V

A

A

L

E

E
|
E

K

E

F

M

G

G

A

V

P

D

K

K

R

G

V

I

S

F

S

S

F

F

V

T

L

L

P

P

L

L

G

G

Y

A

S

T

F

I

N

N

L
x
M

D

D

G

G

S
x
T

M

A

M

L

Y

Y

Q

Q

A

G

F

V

A

T

I

V

M

L

F

F

I

V

A

A

Q

N

A

A

Y

I

N

G

I

H

D

P

L

L

S

T

F

L

T

G

Q

Q

L

L

L

V

I

V

L

V

L

L

T

T

L

A

M

V

I

L

T

A

S

S

K

I

G

G

M

T

A

A

G

G

V
|
V

A
x
P

R
x
G

A
|
A

S
x
G

V

A

V

I

V

M

V

L

A

A

A

M

T

V

L

L

P

Q

M

S

F

V

N

G

L

L

-

D

-

L

-

T

P

P

E

G

A

S

G

P

L

V

L

A

L

L

-

A

-

Y

-

A

-

M

I

I

I

L

G

G

I

I

D

D

Q

A

F

I

L

L

D
|
D

M

M

A

G

R
|
R

T
|
T

A

M

T

V

N
|
N

V

V

V

T

G

G

N

D

S

L

I

A

A

G

T

T

A

V

V

I

V

V

A

A

K

K

S

T

E

E

A

K

E

E

E

L

E

D

P

E

A

S

E

K

binding decyl-beta-d-maltopyranoside: Y190 (≠ F191), L191 (≠ K192), G194 (≠ D195), G195 (≠ Y196), Q198 (≠ A199), R282 (≠ E283), E286 (= E286)
binding (2~{S},3~{S})-2-azanyl-3-[[4-[2-(4-methoxyphenyl)hydrazinyl]phenyl]methoxy]butanedioic acid: R271 (≠ A272), S272 (= S273), S273 (= S274), M307 (≠ L307), T310 (≠ S310), V351 (= V351), P352 (≠ A352), G353 (≠ R353), A354 (= A354), G355 (≠ S355), D391 (= D384), R394 (= R387), T395 (= T388), N398 (= N391)

Sites not aligning to the query:

binding decyl-beta-d-maltopyranoside: 3

6zgbA Glutamate transporter homologue glttk in complex with a photo cage compound (see paper)
32% identity, 96% coverage: 9:413/420 of query aligns to 11:421/425 of 6zgbA

query
sites
6zgbA

R

K

I

I

A

L

V

W

G

G

I

L

A

V

L

L

G

G

V

A

L

V

V

F

G

G

W

L

A

I

C

A

H

G

H

H

Y

F

A

G

G

-

S

-

E

-

Q

-

A

Y

A

A

K

G

A

A

L

V

A

K

G

T

Y

Y

F

I

S

K

M

P

V

F

T

G

D

D

I

L

F

F

L

V

R

R

M

L

I

L

K

K

M

M

I

L

I

V

A

M

P

P

L

I

V

V

F

L

A

A

T

S

L

L

V

V

G

V

G

G

I

A

A

A

S

S

M

I

G

S

N

P

S

A

R

R

S

-

V

L

G

G

R

R

I

V

G

G

M

V

R

K

A

I

M

V

L

V

W

Y

F

Y

V

L

T

A

A

T

S

S

L

A

V

M

S

A

L

V

M

F

L

F

G

G

M

L

A

I

L

V

V

G

N

R

L

L

F

F

Q

N

P

V

G

G

A

A

G

N

L

V

N

N

M

L

-

G

Q

S

V

G

V

T

Q

G

H

K

A

A

T

I

A

E

A

A

V

Q

P

P

V

P

N

-

T

-

G

-

D

-

F

-

S

S

L

L

K

V

T

Q

F

T

I

L

S

L

H

N

V

I

F

V

P

P

R

T

S

N

I

P

A

F

E

A

A

S

M

L

A

A

N

K

N

G

E

E

I

V

L

L

Q

P

I

V

V

I

V

F

F

F

S

A

L

I

F

I

F

L

G

G

F

I

A

A

L

I

A

T

G

Y

V

L

K

M

R

N

A

R

G

N

Y

E

T

E

R

R

I

V

T

R

D

K

T

S

V

A

-

E

-

T

-

L

-

R

-

V

-

F

D

D

E

G

L

L

A

A

K

E

V

A

M

M

F

Y

K

L

I

I

T

V

D

G

Y

G

V

V

M

M

A

Q

F

Y

A

A

P

P

I

I

G

G

V

V

F

F

A

A

A

L

I

I

A

A

S

Y

A

V

I

M

T

A

T

E

N

Q

G

G

L

V

G

R

L

V

A

G

D

P

Y

L

A

A

K

K

L

V

I

V

A

G

E

A

F

V

Y

Y

L

T

G

G

I

L

A

F

L

L

L

Q

W

I

V

V

L

I

L

T

F

Y

A

-

G

-

Y

-

L

-

F

F

L

I

G

L

R

L

S

K

V

V

F

F

T

G

L

I

G

D

-

P

-

I

K

K

L

F

I

I

R

R

-

K

-

A

-

K

E

D

P

A

I

M

L

I

L

T

A

A

F

F

S

V

T

T

A
x
R

S
|
S

E

S

S

G

A

T

Y

L

P

P

K

V

T

T

M

M

E

R

-

V

A

A

L

E

E

E

K

E

F

M

G

G

A

V

P

D

K

K

R

G

V

I

S

F

S

S

F

F

V

T

L

L

P

P

L

L

G

G

Y

A

S

T

F

I

N

N

L
x
M

D

D

G

G

S
x
T

M

A

M

L

Y

Y

Q

Q

A

G

F

V

A

T

I

V

M

L

F

F

I

V

A

A

Q

N

A

A

Y

I

N

G

I

H

D

P

L

L

S

T

F

L

T

G

Q

Q

L

L

L

V

I

V

L

V

L

L

T

T

L

A

M

V

I

L

T

A

S

S

K

I

G

G

M

T

A

A

G

G

V

V

A

P

R

G

A

A

S

G

V

A

V

I

V

M

V

L

A

A

A

M

T

V

L

L

P

Q

M

S

F

V

N

G

L

L

-

D

-

L

-

T

P

P

E

G

A

S

G

P

L

V

L

A

L

L

-

A

-

Y

-

A

-

M

I

I

I

L

G

G

I

I

D

D

Q

A

F

I

L

L

D
|
D

M

M

A

G

R
|
R

T
|
T

A

M

T

V

N

N

V

V

V

T

G

G

N

D

S

L

I

A

A

G

T

T

A

V

V

I

V

V

A

A

K

K

S

T

E

E

A

K

E

E

E

L

E

D

P

E

A

S

E

K

binding (2~{S},3~{S})-2-azanyl-3-[(2-nitrophenyl)methoxy]butanedioic acid: R272 (≠ A272), S273 (= S273), S274 (= S274), M308 (≠ L307), T311 (≠ S310), D392 (= D384), R395 (= R387), T396 (= T388)

5e9sA Crystal structure of substrate-bound glutamate transporter homologue glttk (see paper)
32% identity, 96% coverage: 9:413/420 of query aligns to 13:423/427 of 5e9sA

query
sites
5e9sA

R

K

I

I

A

L

V

W

G

G

I

L

A

V

L

L

G

G

V

A

L

V

V

F

G

G

W

L

A

I

C

A

H

G

H

H

Y

F

A

G

G

-

S

-

E

-

Q

-

A

Y

A

A

K

G

A

A

L

V

A

K

G

T

Y

Y

F

I

S

K

M

P

V

F

T

G

D

D

I

L

F

F

L

V

R

R

M

L

I

L

K

K

M

M

I

L

I

V

A

M

P

P

L

I

V

V

F

L

A

A

T

S

L

L

V

V

G

V

G

G

I

A

A

A

S

S

M

I

G

S

N

P

S

A

R

R

S

-

V

L

G

G

R

R

I

V

G

G

M

V

R

K

A

I

M

V

L

V

W

Y

F
x
Y

V

L

T

A

A
x
T

S
|
S

L

A

V

M

S

A

L

V

M

F

L

F

G

G

M

L

A

I

L

V

V

G

N

R

L

L

F

F

Q

N

P

V

G

G

A

A

G

N

L

V

N

N

M

L

-

G

Q

S

V

G

V

T

Q

G

H

K

A

A

T

I

A

E

A

A

V

Q

P

P

V

P

N

-

T

-

G

-

D

-

F

-

S

S

L

L

K

V

T

Q

F

T

I

L

S

L

H

N

V

I

F

V

P

P

R

T

S

N

I

P

A

F

E

A

A

S

M

L

A

A

N

K

N

G

E

E

I

V

L

L

Q

P

I

V

V

I

V

F

F

F

S

A

L

I