SitesBLAST
Comparing GFF3768 FitnessBrowser__psRCH2:GFF3768 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
5oasA Crystal structure of malate synthase g from pseudomonas aeruginosa in apo form. (see paper)
68% identity, 100% coverage: 1:723/726 of query aligns to 4:724/728 of 5oasA
5vfbA 1.36 angstrom resolution crystal structure of malate synthase g from pseudomonas aeruginosa in complex with glycolic acid.
68% identity, 99% coverage: 2:723/726 of query aligns to 1:720/724 of 5vfbA
P37330 Malate synthase G; MSG; EC 2.3.3.9 from Escherichia coli (strain K12) (see 2 papers)
66% identity, 100% coverage: 1:725/726 of query aligns to 1:723/723 of P37330
- M1 (= M1) modified: Initiator methionine, Removed
- R338 (= R340) mutation to K: Has a specific activity which is only 6.6% of the wild-type activity.
- C617 (= C619) modified: Cysteine sulfenic acid (-SOH); mutation to S: Affinity binding for acetyl-CoA is more than five times greater than that of wild-type, although its specific activity is comparable.
- D631 (= D633) mutation to N: Absence of malate synthase activity.
- C688 (≠ I690) modified: Cysteine sulfenic acid (-SOH)
1d8cA Malate synthase g complexed with magnesium and glyoxylate (see paper)
66% identity, 99% coverage: 3:724/726 of query aligns to 1:709/709 of 1d8cA
- active site: D268 (= D272), E270 (= E274), R325 (= R340), E414 (= E429), D442 (= D457), D618 (= D633)
- binding glyoxylic acid: M412 (= M427), E414 (= E429), G439 (= G454), L441 (= L456), D442 (= D457)
- binding magnesium ion: E414 (= E429), D442 (= D457)
- binding sorbitol: Q59 (= Q61), H449 (= H464), V688 (≠ R703), K689 (≠ E704), Q690 (= Q705), P691 (= P706)
1p7tA Structure of escherichia coli malate synthase g:pyruvate:acetyl- coenzyme a abortive ternary complex at 1.95 angstrom resolution (see paper)
66% identity, 99% coverage: 5:724/726 of query aligns to 2:706/706 of 1p7tA
- active site: D259 (= D272), E261 (= E274), R322 (= R340), E411 (= E429), D439 (= D457), D615 (= D633)
- binding acetyl coenzyme *a: V115 (= V118), V116 (= V119), P117 (= P120), R122 (= R125), Y123 (= Y126), N126 (= N129), A127 (= A130), S263 (= S276), R295 (= R313), R322 (= R340), M492 (= M510), P520 (= P538), P522 (= P540), C601 (= C619), M613 (= M631), D615 (= D633)
- binding magnesium ion: E411 (= E429), D439 (= D457)
- binding pyruvic acid: E411 (= E429), G436 (= G454), L438 (= L456), D439 (= D457), W518 (= W536)
6axeA Crystal structure of a malate synthase g from mycobacterium marinum bound to acetyl coa
62% identity, 100% coverage: 1:723/726 of query aligns to 2:726/729 of 6axeA
- active site: D272 (= D272), E274 (= E274), R338 (= R340), E437 (= E429), D465 (= D457), D636 (= D633)
- binding acetyl coenzyme *a: V119 (= V118), V120 (= V119), R126 (= R125), F127 (≠ Y126), N130 (= N129), A131 (= A130), R311 (= R313), R338 (= R340), M518 (= M510), W544 (= W536), P546 (= P538), P548 (= P540), C622 (= C619), K624 (= K621), M634 (= M631), D636 (= D633)
- binding magnesium ion: E437 (= E429), D465 (= D457)
P9WK16 Malate synthase G; EC 2.3.3.9 from Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) (see paper)
61% identity, 100% coverage: 1:726/726 of query aligns to 1:726/741 of P9WK16
- R339 (= R340) active site, Proton acceptor
- D633 (= D633) active site, Proton donor
2gq3A Mycobacterium tuberculosis malate synthase in complex with magnesium, malate, and coenzyme a (see paper)
61% identity, 100% coverage: 2:726/726 of query aligns to 1:719/720 of 2gq3A
- active site: D267 (= D272), E269 (= E274), R335 (= R340), E430 (= E429), D458 (= D457), D626 (= D633)
- binding coenzyme a: V114 (= V118), V115 (= V119), R121 (= R125), F122 (≠ Y126), N125 (= N129), A126 (= A130), K301 (= K306), R308 (= R313), P539 (= P538), P541 (= P540), C612 (= C619), M624 (= M631), D626 (= D633)
- binding magnesium ion: H370 (= H375), K373 (≠ Q378), N378 (vs. gap), G379 (vs. gap), L381 (vs. gap), E430 (= E429), D458 (= D457)
6dkoA Crystal structure of mycobacterium tuberculosis malate synthase in complex with 2,6-f-phenyldiketoacid (see paper)
61% identity, 100% coverage: 2:726/726 of query aligns to 1:715/716 of 6dkoA
- active site: D270 (= D272), E272 (= E274), R332 (= R340), E424 (= E429), D452 (= D457), D622 (= D633)
- binding 4-(2,6-difluorophenyl)-2,4-dioxobutanoic acid: R332 (= R340), G449 (= G454), L451 (= L456), D452 (= D457), M505 (= M510), W531 (= W536), M620 (= M631), D622 (= D633)
- binding magnesium ion: E424 (= E429), D452 (= D457)
6c2xA Crystal structure of mycobacterium tuberculosis malate synthase in complex with 2-br-6-me-phenyldiketoacid (see paper)
61% identity, 100% coverage: 2:726/726 of query aligns to 1:710/711 of 6c2xA
- active site: D268 (= D272), E270 (= E274), R328 (= R340), E418 (= E429), D446 (= D457), D617 (= D633)
- binding (2S)-4-(2-bromo-6-methylphenyl)-2-hydroxy-4-oxobutanoic acid: V115 (= V118), R328 (= R340), E418 (= E429), G443 (= G454), L445 (= L456), D446 (= D457), M499 (= M510), W525 (= W536), M615 (= M631), D617 (= D633)
- binding magnesium ion: E418 (= E429), D446 (= D457)
6dnpA Crystal structure of mycobacterium tuberculosis malate synthase in complex with 2-f-3-methyl-6-f-phenyldiketoacid (see paper)
61% identity, 100% coverage: 2:726/726 of query aligns to 1:711/712 of 6dnpA
- active site: D270 (= D272), E272 (= E274), R328 (= R340), E419 (= E429), D447 (= D457), D618 (= D633)
- binding (2Z)-4-(2,6-difluoro-3-methylphenyl)-2-hydroxy-4-oxobut-2-enoic acid: R328 (= R340), E419 (= E429), G444 (= G454), L446 (= L456), D447 (= D457), M500 (= M510), W526 (= W536), M616 (= M631), D618 (= D633)
- binding magnesium ion: E419 (= E429), D447 (= D457)
5e9xA Crystal structure of mycobacterium tuberculosis malate synthase in complex with 2-chloro-6h-thieno[2,3-b]pyrrole-5-carboxylic acid (see paper)
61% identity, 100% coverage: 2:726/726 of query aligns to 1:714/715 of 5e9xA
- active site: D267 (= D272), E269 (= E274), R327 (= R340), E422 (= E429), D450 (= D457), D621 (= D633)
- binding 2-chloranyl-6~{H}-thieno[2,3-b]pyrrole-5-carboxylic acid: V114 (= V118), S271 (= S276), M503 (= M510), M619 (= M631), E620 (= E632), D621 (= D633)
- binding magnesium ion: E422 (= E429), D450 (= D457)
5cahA Crystal structure of mycobacterium tuberculosis malate synthase in complex with 6h-thieno[2,3-b]pyrrole-5-carboxylic acid (see paper)
61% identity, 100% coverage: 2:726/726 of query aligns to 1:705/706 of 5cahA
- active site: D266 (= D272), E268 (= E274), R324 (= R340), E413 (= E429), D441 (= D457), D612 (= D633)
- binding 6H-thieno[2,3-b]pyrrole-5-carboxylic acid: S270 (= S276), M494 (= M510), M610 (= M631), E611 (= E632), D612 (= D633)
- binding magnesium ion: E413 (= E429), D441 (= D457)
5c9wA Crystal structure of mycobacterium tuberculosis malate synthase in complex with (z)-n-(2-bromophenyl)-2-(hydroxyimino)acetamide (see paper)
61% identity, 100% coverage: 2:726/726 of query aligns to 1:712/713 of 5c9wA
- active site: D269 (= D272), E271 (= E274), R327 (= R340), E420 (= E429), D448 (= D457), D619 (= D633)
- binding (2E)-N-(2-bromophenyl)-2-(hydroxyimino)acetamide: V116 (= V118), R327 (= R340), M501 (= M510), W527 (= W536), M617 (= M631), D619 (= D633)
- binding magnesium ion: E420 (= E429), D448 (= D457), V464 (≠ L473), R465 (= R474), T468 (≠ D477)
5c9rA Crystal structure of mycobacterium tuberculosis malate synthase in complex with 3-((4-chlorophenyl)thio)propanoic acid (see paper)
61% identity, 100% coverage: 2:726/726 of query aligns to 1:716/717 of 5c9rA
- active site: D270 (= D272), E272 (= E274), R331 (= R340), E424 (= E429), D452 (= D457), D623 (= D633)
- binding 3-[(4-chlorophenyl)sulfanyl]propanoic acid: V117 (= V118), E424 (= E429), D452 (= D457), M505 (= M510), W531 (= W536), M621 (= M631), D623 (= D633)
- binding magnesium ion: E424 (= E429), D452 (= D457)
5drcA Crystal structure of mycobacterium tuberculosis malate synthase in complex with 2-hydroxy-4-(1h-indol-3-yl)-4-oxobut-2-enoic acid (see paper)
61% identity, 100% coverage: 2:726/726 of query aligns to 1:715/716 of 5drcA
- active site: D270 (= D272), E272 (= E274), R330 (= R340), E423 (= E429), D451 (= D457), D622 (= D633)
- binding (2Z)-2-hydroxy-4-(1H-indol-3-yl)-4-oxobut-2-enoic acid: R330 (= R340), E423 (= E429), G448 (= G454), L450 (= L456), D451 (= D457), M504 (= M510), W530 (= W536), M620 (= M631), D622 (= D633)
- binding magnesium ion: E423 (= E429), D451 (= D457), R468 (= R474), T471 (≠ D477)
5cjmA Crystal structure of mycobacterium tuberculosis malate synthase in complex with 4h-thieno[3,2-b]pyrrole-5-carboxylic acid (see paper)
61% identity, 100% coverage: 2:726/726 of query aligns to 1:710/711 of 5cjmA
- active site: D270 (= D272), E272 (= E274), R328 (= R340), E418 (= E429), D446 (= D457), D617 (= D633)
- binding 4h-thieno[3,2-b]pyrole-5-carboxylic acid: V117 (= V118), S274 (= S276), M499 (= M510), E616 (= E632), D617 (= D633)
- binding magnesium ion: E418 (= E429), D446 (= D457)
6bu1A Crystal structure of mycobacterium tuberculosis malate synthase in complex with 2-br-3-oh-phenyldiketoacid (see paper)
61% identity, 100% coverage: 3:726/726 of query aligns to 1:711/712 of 6bu1A
- active site: D269 (= D272), E271 (= E274), R327 (= R340), E419 (= E429), D447 (= D457), D618 (= D633)
- binding (2Z)-4-(2-bromo-3-hydroxyphenyl)-2-hydroxy-4-oxobut-2-enoic acid: V116 (= V118), R327 (= R340), E419 (= E429), G444 (= G454), L446 (= L456), D447 (= D457), M500 (= M510), W526 (= W536), M616 (= M631), D618 (= D633)
- binding magnesium ion: E419 (= E429), D447 (= D457), V463 (≠ L473), R464 (= R474)
5cczA Crystal structure of mycobacterium tuberculosis malate synthase in complex with 3-(4-fluorophenyl)-4-methyl-1h-pyrazol-5-amine (see paper)
60% identity, 100% coverage: 2:726/726 of query aligns to 1:712/713 of 5cczA
- active site: D267 (= D272), E269 (= E274), R325 (= R340), E420 (= E429), D448 (= D457), D619 (= D633)
- binding 3-(4-fluorophenyl)-4-methyl-1H-pyrazol-5-amine: V114 (= V118), S271 (= S276), M501 (= M510), W527 (= W536), P529 (= P538), M617 (= M631), E618 (= E632), D619 (= D633)
- binding magnesium ion: E420 (= E429), D448 (= D457)
5cbjA Crystal structure of mycobacterium tuberculosis malate synthase in complex with 3-(phenylthio)acrylic acid (see paper)
60% identity, 100% coverage: 2:726/726 of query aligns to 1:708/709 of 5cbjA
- active site: D265 (= D272), E267 (= E274), R323 (= R340), E416 (= E429), D444 (= D457), D615 (= D633)
- binding (2E)-3-(phenylsulfanyl)prop-2-enoic acid: V112 (= V118), M497 (= M510), W523 (= W536), P525 (= P538), M613 (= M631), E614 (= E632), D615 (= D633)
- binding magnesium ion: E416 (= E429), D444 (= D457)
Query Sequence
>GFF3768 FitnessBrowser__psRCH2:GFF3768
MTERVTLGRLQVAANLQRFIEDEVLPGSGIEAAAFWQGLDALVHDLAPKNRELLAERDRM
QVELDTWHKANPGPIKDMAAYRAFLESIGYLLPVPGEVKIETANVDSEIATQAGPQLVVP
IMNARYALNAANARWGSLYDALYGTDAISEEGGAQKGPGYNEVRGAKVIAYARNFLDQAA
PLAEGSHADATAYRVQDGQLKVTLENGSVTGLAQPEKFVGFQGEAVEPKAVLLKNNGLHV
EIQIDPNSPIGQTDAAGVKDLLVESAVSTILDCEDSVAAVDADDKVLAYRNWLGIVQGTL
SEEVAKGSSSFVRRLNPDREYSAPNGGELKLHGRSLLFIRNVGHLMTNPAILLEDGREIP
EGIMDGVFTSLIAKHDLQRKGNSRTGSVYIVKPKMHGPKEVAFADELFGRVEELIGVPRY
TLKMGIMDEERRTSANLKACIAAAKHRVAFINTGFLDRTGDEMHTCMEAGPVLRKGDMKS
TPWIQSYERNNVLVGLACGLRGKAQIGKGMWAMPDLMAAMLEQKIGHPKSGANTAWVPSP
TGATLHALHYHQVNVQAVQSELEKIDLAAERDQLLNDLLSIPVVAEDKWSAEEKQQELDN
NCQGILGYVVRWVEQGVGCSKVPDIHNVGLMEDRATLRISSQHIANWLHHGVVSREQVQE
TLQRMAKVVDQQNAGDPLYRAMSSDYTQSIAFQAASDLVFKGREQPAGYTEPLLHAWRQR
YKASQA
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory