SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF3770 FitnessBrowser__psRCH2:GFF3770 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

5odqB Heterodisulfide reductase / [nife]-hydrogenase complex from methanothermococcus thermolithotrophicus soaked with bromoethanesulfonate. (see paper)
24% identity, 52% coverage: 175:386/405 of query aligns to 8:239/291 of 5odqB

query
sites
5odqB

G
|
G

C
|
C

V

I

Q

M

P

P

S

N

L

R

S

Y

P

A

S

G

T

V

N

E

A

A

A

T

A

R

R

T

V

V

L

M

D

E

R

K

L

L

G

G

I

V

S

E

V

L

S

V

P

D

A

M

R

T

E

G

A

A

G

S

C
|
C

-

P

-

A

-

P

-
x
G

-

V

-

F

G

G

A

S

V

F

D

D

Y

-

H

-

L

-

N

-

A

-

Q

Q

D

K

A

T

G

W

L

L

D

T

R

L

A

A

R

A

R

R

N

N

I

L

D

-

A

-

W

-

W

C

P

I

A

A

I

E

E

E

A

M

G

G

A

V

E

D

A

-

I

I

V

V

Q

T

T

V

A
x
C

S

N

G
|
G

C
|
C

G

Y

A

G

F

S

V

L

K

F

E

E

Y

A

G

A

H

H

L

L

K

H

D

D

D

N

P

K

A

E

-

A

-

L

-

N

-

F

Y

V

A

N

A

E

K

K

A

L

A

D

R

K

V

V

S

G

E

K

L

E

A

Y

K

K

D

G

L

N

V

V

E

K

V

V

L

R

R

H

S

F

A

A

E

E

L

L

E

I

K

Y

L

N

N

D

V

I

R

G

A

V

D

D

K

K

-

I

-

A

-

E

-

K

-

V

-

E

-

R

-

P

-

L

-

N

-

I

R

N

M

V

A

G

F

V

H

H

C

Y

P
x
G

C
|
C

T
x
H

L

F

-

L

-

K

-

P

Q

T

H

D

A

V

Q

K

K

H

L

L

G

G

G

S

A

A

-

E

-

R

-

P

-

V

-

M

V

L

E

D

D

E

V

I

L

V

T

E

R

A

L

T

G

G

Y

A

Q

K

L

S

T

V

A

P

V

Y

P

A

D

D

A

K

H

M

L

M

C
|
C

G

G

S

A

A
x
G

G

G

S

G

Y

V

S
x
R

I

A

T

R

Q

E

P

L

E

E

I

L

S

S

H

L

Q

D

L

M

R

T

D

N

N

E

K

K

L

I

N

E

A

N

L

M

E

I

S

K

G

A

K

G

P

A

E

D

V

C

I

T

V

V

T

N

A

V

N
x
C

I

P

G
x
F

C
|
C

Q

H

T
x
L

H

Q

L

F

D

D

binding Non-cubane [4Fe-4S]-cluster: G8 (= G175), C9 (= C176), C41 (= C208), C42 (= C209), C78 (≠ A248), G80 (= G250), C81 (= C251), G152 (≠ P307), C153 (= C308), H154 (≠ T309), C193 (= C340), C194 (= C341), C231 (≠ N378), F233 (≠ G380), C234 (= C381)
binding 2-bromanylethanesulfonic acid: G46 (vs. gap), G80 (= G250), H154 (≠ T309), G197 (≠ A344), R201 (≠ S348), F233 (≠ G380), L236 (≠ T383)

5odhH Heterodisulfide reductase / [nife]-hydrogenase complex from methanothermococcus thermolithotrophicus soaked with heterodisulfide for 3.5 minutes (see paper)
24% identity, 52% coverage: 175:386/405 of query aligns to 8:239/291 of 5odhH

query
sites
5odhH

G
|
G

C
|
C

V

I

Q

M

P

P

S

N

L

R

S

Y

P

A

S

G

T

V

N

E

A

A

A

T

A

R

R

T

V

V

L

M

D

E

R

K

L

L

G

G

I

V

S

E

V

L

S

V

P

D

A

M

R

T

E

G

A

A

G

S

C
|
C

-

P

-
x
A

-
x
P

-
x
G

-

V

-

F

G

G

A

S

V

F

D

D

Y

-

H

-

L

-

N

-

A

-

Q

Q

D

K

A

T

G

W

L

L

D

T

R

L

A

A

R

A

R

R

N

N

I

L

D

-

A

-

W

-

W

C

P

I

A

A

I

E

E

E

A

M

G

G

A

V

E

D

A

-

I

I

V

V

Q

T

T

V

A
x
C

S

N

G
|
G

C
|
C

G

Y

A

G

F

S

V

L

K

F

E

E

Y

A

G

A

H

H

L

L

K

H

D

D

D

N

P

K

A

E

-

A

-

L

-

N

-

F

Y

V

A

N

A

E

K

K

A

L

A

D

R

K

V

V

S

G

E

K

L

E

A

Y

K

K

D

G

L

N

V

V

E

K

V

V

L

R

R

H

S

F

A

A

E

E

L

L

E

I

K

Y

L

N

N

D

V

I

R

G

A

V

D

D

K

K

-

I

-

A

-

E

-

K

-

V

-

E

-

R

-

P

-

L

-

N

-

I

R

N

M

V

A

G

F

V

H

H

C

Y

P

G

C
|
C

T
x
H

L

F

-

L

-

K

-

P

Q

T

H

D

A

V

Q

K

K

H

L

L

G

G

G

S

A

A

-

E

-

R

-

P

-

V

-

M

V

L

E

D

D

E

V

I

L

V

T

E

R

A

L

T

G

G

Y

A

Q

K

L

S

T

V

A

P

V

Y

P

A

D

D

A

K

H

M

L

M

C
|
C

G

G

S

A

A
x
G

G
|
G

S

G

Y

V

S
x
R

I

A

T

R

Q

E

P

L

E

E

I

L

S

S

H

L

Q

D

L

M

R

T

D

N

N

E

K

K

L

I

N

E

A

N

L

M

E

I

S

K

G

A

K

G

P

A

E

D

V

C

I

T

V

V

T

N

A

V

N
x
C

I

P

G
x
F

C
|
C

Q

H

T

L

H

Q

L

F

D

D

binding Non-cubane [4Fe-4S]-cluster: G8 (= G175), C9 (= C176), C41 (= C208), C42 (= C209), C78 (≠ A248), G80 (= G250), C81 (= C251), C153 (= C308), H154 (≠ T309), C193 (= C340), C194 (= C341), C231 (≠ N378), F233 (≠ G380), C234 (= C381)
binding 1-thioethanesulfonic acid: A44 (vs. gap), P45 (vs. gap), G46 (vs. gap), G80 (= G250), H154 (≠ T309), G197 (≠ A344), G198 (= G345)
binding Coenzyme B: R201 (≠ S348), F233 (≠ G380)

Sites not aligning to the query:

binding Coenzyme B: 240

5odhB Heterodisulfide reductase / [nife]-hydrogenase complex from methanothermococcus thermolithotrophicus soaked with heterodisulfide for 3.5 minutes (see paper)
24% identity, 52% coverage: 175:386/405 of query aligns to 8:239/291 of 5odhB

query
sites
5odhB

G
|
G

C
|
C

V
x
I

Q

M

P

P

S

N

L

R

S

Y

P

A

S

G

T

V

N

E

A

A

A

T

A

R

R

T

V

V

L

M

D

E

R

K

L

L

G

G

I

V

S

E

V

L

S

V

P

D

A

M

R

T

E

G

A

A

G

S

C
|
C

-

P

-

A

-
x
P

-
x
G

-

V

-

F

G

G

A

S

V

F

D

D

Y

-

H

-

L

-

N

-

A

-

Q

Q

D

K

A

T

G

W

L

L

D

T

R

L

A

A

R

A

R

R

N

N

I

L

D

-

A

-

W

-

W

C

P

I

A

A

I

E

E

E

A

M

G

G

A

V

E

D

A

-

I

I

V

V

Q

T

T

V

A
x
C

S

N

G
|
G

C
|
C

G

Y

A

G

F

S

V

L

K

F

E

E

Y

A

G

A

H

H

L

L

K

H

D

D

D

N

P

K

A

E

-

A

-

L

-

N

-

F

Y

V

A

N

A

E

K

K

A

L

A

D

R

K

V

V

S

G

E

K

L

E

A

Y

K

K

D

G

L

N

V

V

E

K

V

V

L

R

R

H

S

F

A

A

E

E

L

L

E

I

K

Y

L

N

N

D

V

I

R

G

A

V

D

D

K

K

-

I

-

A

-

E

-

K

-

V

-

E

-

R

-

P

-

L

-

N

-

I

R

N

M

V

A

G

F

V

H

H

C

Y

P
x
G

C
|
C

T
x
H

L

F

-

L

-

K

-

P

Q

T

H

D

A

V

Q

K

K

H

L

L

G

G

G

S

A

A

-

E

-

R

-

P

-

V

-

M

V

L

E

D

D

E

V

I

L

V

T

E

R

A

L

T

G

G

Y

A

Q

K

L

S

T

V

A

P

V

Y

P

A

D

D

A

K

H

M

L

M

C
|
C

G

G

S

A

A

G

G

G

S

G

Y

V

S

R

I

A

T

R

Q

E

P

L

E

E

I

L

S

S

H

L

Q

D

L

M

R

T

D

N

N

E

K

K

L

I

N

E

A

N

L

M

E

I

S

K

G

A

K

G

P

A

E

D

V

C

I

T

V

V

T

N

A

V

N
x
C

I

P

G
x
F

C
|
C

Q

H

T

L

H

Q

L

F

D

D

binding Non-cubane [4Fe-4S]-cluster: G8 (= G175), C9 (= C176), I10 (≠ V177), C41 (= C208), C42 (= C209), C78 (≠ A248), G80 (= G250), C81 (= C251), G152 (≠ P307), C153 (= C308), H154 (≠ T309), C193 (= C340), C194 (= C341), C231 (≠ N378), F233 (≠ G380), C234 (= C381)
binding 1-thioethanesulfonic acid: P45 (vs. gap), G46 (vs. gap), G80 (= G250), F233 (≠ G380)

5odcB Heterodisulfide reductase / [nife]-hydrogenase complex from methanothermococcus thermolithotrophicus at 2.3 a resolution (see paper)
24% identity, 52% coverage: 175:386/405 of query aligns to 8:239/291 of 5odcB

query
sites
5odcB

G
|
G

C
|
C

V
x
I

Q

M

P

P

S

N

L

R

S

Y

P

A

S

G

T

V

N

E

A

A

A

T

A

R

R

T

V

V

L

M

D

E

R

K

L

L

G

G

I

V

S

E

V

L

S

V

P

D

A

M

R

T

E

G

A

A

G

S

C
|
C

-

P

-

A

-

P

-

G

-

V

-

F

G

G

A

S

V

F

D

D

Y

-

H

-

L

-

N

-

A

-

Q

Q

D

K

A

T

G

W

L

L

D

T

R

L

A

A

R

A

R

R

N

N

I

L

D

-

A

-

W

-

W

C

P

I

A

A

I

E

E

E

A

M

G

G

A

V

E

D

A

-

I

I

V

V

Q

T

T

V

A
x
C

S
x
N

G
|
G

C
|
C

G

Y

A

G

F

S

V

L

K

F

E

E

Y

A

G

A

H

H

L

L

K

H

D

D

D

N

P

K

A

E

-

A

-

L

-

N

-

F

Y

V

A

N

A

E

K

K

A

L

A

D

R

K

V

V

S

G

E

K

L

E

A

Y

K

K

D

G

L

N

V

V

E

K

V

V

L

R

R

H

S

F

A

A

E

E

L

L

E

I

K

Y

L

N

N

D

V

I

R

G

A

V

D

D

K

K

-

I

-

A

-

E

-

K

-

V

-

E

-

R

-

P

-

L

-

N

-

I

R

N

M

V

A

G

F

V

H

H

C

Y

P
x
G

C
|
C

T

H

L

F

-

L

-

K

-

P

Q

T

H

D

A

V

Q

K

K

H

L

L

G

G

G

S

A

A

-

E

-

R

-

P

-

V

-

M

V

L

E

D

D

E

V

I

L

V

T

E

R

A

L

T

G

G

Y

A

Q

K

L

S

T

V

A

P

V

Y

P

A

D

D

A

K

H

M

L

M

C
|
C

G

G

S

A

A
x
G

G

G

S

G

Y

V

S

R

I

A

T

R

Q

E

P

L

E

E

I

L

S

S

H

L

Q

D

L

M

R

T

D

N

N

E

K

K

L

I

N

E

A

N

L

M

E

I

S

K

G

A

K

G

P

A

E

D

V

C

I

T

V

V

T

N

A

V

N
x
C

I

P

G
x
F

C
|
C

Q

H

T

L

H

Q

L

F

D

D

binding Non-cubane [4Fe-4S]-cluster: G8 (= G175), C9 (= C176), I10 (≠ V177), C41 (= C208), C42 (= C209), C78 (≠ A248), N79 (≠ S249), G80 (= G250), C81 (= C251), G152 (≠ P307), C153 (= C308), C193 (= C340), C194 (= C341), G197 (≠ A344), C231 (≠ N378), F233 (≠ G380), C234 (= C381)

8b6gCB Succinate dehydrogenase (quinone) (see paper)
26% identity, 26% coverage: 2:108/405 of query aligns to 163:283/285 of 8b6gCB

query
sites
8b6gCB

Q

K

T

T

N

P

L

K

S

P

E

D

A

A

A

N

K

K

K

E

L

Y

P

P

R

Q

A

S

E

P

E

E

A

Q

E

R

S

A

I

L

L

L

-

D

-

G

-

L

R

Y

S

E

C
|
C

V

V

H
x
L

C
|
C

G

A

F

A

C
|
C

N

S

A

T

T

S

C
|
C

P
|
P

T
x
S

Y

Y

Q

W

L
x
W

L

H

G

P

D

D

E

R

L

Y

D

L

G

G

P

P

R

A

G

-

R

-

I

-

Y

-

L

I

M

L

K

Q

Q

Q

M

A

F

Y

E

R

G

W

-
x
I

-

V

-
x
D

-

S

-

R

G
x
D

E

E

V

Y

T
|
T

E

Q

-

E

-

R

-

I

-

E

-

R

-

I

S

A

T

E

Q

D

L

V

H

R

L

L

D

D

R

D

C
|
C

L
x
Q

T
x
Q

C
x
I

R
x
G

N
x
M

C
|
C

E

S

T

F

T

T

C
|
C

P

P

S
x
K

G

G

V

L

K

N

-

P

-

Q

-

L

-

S

Y

L

H

K

N

N

L

L

L

M

D

D

I

M

G

V

R

K

D

D

F

F

I

R

E

Q

Q

K

Q

R

V

I

Q

E

R

Q

binding calcium ion: I222 (vs. gap), D224 (vs. gap), D227 (≠ G62), T230 (= T65)
binding fe3-s4 cluster: C199 (= C35), S201 (≠ T37), C246 (= C75), Q247 (≠ L76), Q248 (≠ T77), I249 (≠ C78), G250 (≠ R79), M251 (≠ N80), C252 (= C81)
binding iron/sulfur cluster: C189 (= C25), L191 (≠ H27), C192 (= C28), C195 (= C31), C256 (= C85), K258 (≠ S87)
binding Ubiquinone-8: P200 (= P36), W204 (≠ L40)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 96, 97, 99, 101, 102, 104, 116

8gymsb NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial (see paper)
26% identity, 26% coverage: 2:108/405 of query aligns to 156:276/279 of 8gymsb

query
sites
8gymsb

Q

K

T

T

N

P

L

K

S

P

E

D

A

A

A

N

K

K

K

E

L

Y

P

P

R

Q

A

S

E

P

E

E

A

Q

E

R

S

A

I

L

L

L

-

D

-

G

-

L

R

Y

S

E

C
|
C

V
|
V

H

L

C
|
C

G
x
A

F

A

C
|
C

N

S

A

T

T

S

C
|
C

P
|
P

T
x
S

Y

Y

Q

W

L
x
W

L

H

G

P

D

D

E

R

L

Y

D

L

G

G

P

P

R
x
A

G

-

R

-

I

-

Y

-

L

I

M

L

K

Q

Q

Q

M

A

F

Y

E

R

G

W

-

I

-

V

-

D

-

S

-

R

G

D

E

E

V

Y

T

T

E

Q

-

E

-

R

-

I

-

E

-

R

-

I

S

A

T

E

Q

D

L

V

H

R

L

L

D

D

R

D

C
|
C

L
x
Q

T
x
Q

C
x
I

R
x
G

N
x
M

C
|
C

E

S

T

F

T

T

C
|
C

P

P

S

K

G

G

V

L

K

N

-

P

-
x
Q

-

L

-

S

Y

L

H

K

N

N

L

L

L

M

D

D

I

M

G

V

R

K

D

D

F

F

I

R

E

Q

Q

K

Q

R

V

I

Q

E

R

Q

binding fe3-s4 cluster: C192 (= C35), S194 (≠ T37), C239 (= C75), Q240 (≠ L76), Q241 (≠ T77), I242 (≠ C78), G243 (≠ R79), M244 (≠ N80), C245 (= C81), Q256 (vs. gap)
binding iron/sulfur cluster: C182 (= C25), V183 (= V26), C185 (= C28), A186 (≠ G29), C188 (= C31), A206 (≠ R49), C249 (= C85)
binding ubiquinone-10: P193 (= P36), W197 (≠ L40), Q240 (≠ L76), I242 (≠ C78)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 89, 90, 94, 95, 97, 107, 109

Q007T0 Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial; Iron-sulfur subunit of complex II; Ip; EC 1.3.5.1 from Sus scrofa (Pig) (see paper)
32% identity, 19% coverage: 12:89/405 of query aligns to 171:257/280 of Q007T0

query
sites
Q007T0

L

L

P

Q

R

S

A

I

E

E

A

R

E

E

S

K

I

L

-

D

-

G

L

L

R

Y

S

E

C
|
C

V

I

H

L

C
|
C

G

A

F

C

C
|
C

N

S

A

T

T

S

C
|
C

P

P

T

S

Y

Y

Q

W

L
x
W

L

N

G

G

D

D

E

K

L

Y

D

L

G

G

P

P

R

A

G

V

R

L

I

M

Y

Q

L

A

M

Y

K

R

Q

W

M

M

F

I

E

D

G

S

-

R

G

D

E

D

V

F

T

T

E

E

-

E

-

R

-

L

-

A

-

K

-

L

S

Q

T

D

Q

P

L

F

H

S

L

L

D

Y

R

R

C
|
C

L

H

T

T

C

I

R

M

N

N

C
|
C

E

T

T

G

T

T

C
|
C

P

P

S

K

G

G

V

L

C186 (= C25) binding
C189 (= C28) binding
C192 (= C31) binding
C196 (= C35) binding
W201 (≠ L40) binding
C243 (= C75) binding
C249 (= C81) binding
C253 (= C85) binding

Sites not aligning to the query:

93 binding
98 binding
101 binding
113 binding

8gs8B Cryo-em structure of the human respiratory complex ii (see paper)
32% identity, 19% coverage: 12:89/405 of query aligns to 137:223/239 of 8gs8B

query
sites
8gs8B

L

L

P

Q

R

S

A

I

E

E

A

R

E

E

S

K

I

L

-

D

-

G

L

L

R

Y

S

E

C
|
C

V

I

H

L

C
|
C

G

A

F

C

C
|
C

N

S

A

T

T

S

C
|
C

P
|
P

T

S

Y

Y

Q

W

L
x
W

L

N

G

G

D

D

E

K

L
x
Y

D

L

G

G

P
|
P

R
x
A

G

V

R

L

I

M

Y

Q

L

A

M

Y

K

R

Q

W

M

M

F

I

E

D

G

S

-

R

G

D

E

D

V

F

T

T

E

E

-

E

-

R

-

L

-

A

-

K

-

L

S

Q

T

D

Q

P

L

F

H

S

L

L

D

Y

R

R

C
|
C

L
x
H

T
|
T

C
x
I

R
x
M

N
|
N

C
|
C

E

T

T

R

T

T

C
|
C

P

P

S

K

G

G

V

L

binding fe3-s4 cluster: C162 (= C35), Y172 (≠ L45), P175 (= P48), C209 (= C75), H210 (≠ L76), T211 (= T77), I212 (≠ C78), M213 (≠ R79), N214 (= N80), C215 (= C81)
binding iron/sulfur cluster: C152 (= C25), C155 (= C28), C158 (= C31), A176 (≠ R49), C219 (= C85)
binding ubiquinone-1: P163 (= P36), W167 (≠ L40), I212 (≠ C78)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 59, 60, 64, 65, 66, 67, 79

5t61L Tungsten formylmethanofuran dehydrogenase subunit fwdF (see paper)
30% identity, 20% coverage: 25:105/405 of query aligns to 70:139/348 of 5t61L

query
sites
5t61L

C
|
C

V
|
V

H
x
L

C
|
C

G
|
G

F

M

C
|
C

N

S

A

S

T

I

C
|
C

P

P

T

-

Y

F

Q

Q

L

A

L
|
L

G

D

D

L

E

Q

L

I

D

D

G

G

P

T

R

S

G

-

R

-

I

-

Y

-

L

-

M

I

K

K

Q

E

M

L

F

A

E

E

G

Y

G

P

E

K

V

I

T

I

E

K

S

S

T

A

Q

E

L

I

H

D

L

D

D

E

R

T

C
|
C

L

I

T

Q

C
|
C

R
x
K

N

A

C
|
C

E

E

T

T

T

A

C
|
C

P

P

S

-

G

-

V

-

K

-

Y

-

H

Q

N

D

L

A

L
x
I

D

T

I

I

G

T

R

R

D

E

F

L

I

P

E

E

Q

R

Q

K

binding iron/sulfur cluster: C70 (= C25), V71 (= V26), L72 (≠ H27), C73 (= C28), G74 (= G29), C76 (= C31), C80 (= C35), L85 (= L41), C114 (= C75), C117 (= C78), K118 (≠ R79), C120 (= C81), C124 (= C85), I129 (≠ L95)

Sites not aligning to the query:

binding iron/sulfur cluster: 30, 31, 32, 33, 34, 36, 40, 41, 146, 153, 156, 159, 163, 164, 168, 186, 193, 195, 196, 197, 199, 203, 204, 208, 212, 215, 238, 239, 240, 241, 242, 244, 248, 249, 270, 272, 273, 274, 276, 280, 281, 284, 307, 308, 309, 310, 311, 313, 317

3sfeB Crystal structure of porcine mitochondrial respiratory complex ii bound with oxaloacetate and thiabendazole (see paper)
32% identity, 19% coverage: 12:89/405 of query aligns to 136:222/240 of 3sfeB

query
sites
3sfeB

L

L

P

Q

R

S

A

I

E

E

A

R

E

E

S

K

I

L

-

D

-

G

L

L

R

Y

S

E

C
|
C

V
x
I

H
x
L

C
|
C

G
x
A

F

C

C
|
C

N

S

A

T

T

S

C
|
C

P

P

T

S

Y

Y

Q

W

L

W

L

N

G

G

D

D

E

K

L
x
Y

D

L

G

G

P
|
P

R
x
A

G

V

R

L

I

M

Y

Q

L

A

M

Y

K

R

Q

W

M

M

F

I

E

D

G

S

-

R

G

D

E

D

V

F

T

T

E

E

-

E

-

R

-

L

-

A

-

K

-

L

S

Q

T

D

Q

P

L

F

H

S

L

L

D

Y

R

R

C
|
C

L
x
H

T
|
T

C
x
I

R
x
M

N
|
N

C
|
C

E

T

T

G

T

T

C
|
C

P
|
P

S
x
K

G

G

V
x
L

binding fe3-s4 cluster: C161 (= C35), Y171 (≠ L45), P174 (= P48), C208 (= C75), T210 (= T77), I211 (≠ C78), M212 (≠ R79), N213 (= N80), C214 (= C81)
binding iron/sulfur cluster: C151 (= C25), I152 (≠ V26), L153 (≠ H27), C154 (= C28), A155 (≠ G29), C157 (= C31), A175 (≠ R49), C218 (= C85), P219 (= P86), K220 (≠ S87), L222 (≠ V89)
binding 2-(1,3-thiazol-4-yl)-1h-benzimidazole: H209 (≠ L76), I211 (≠ C78)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 58, 59, 61, 63, 64, 65, 66, 78

Q9YHT2 Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial; Iron-sulfur subunit of complex II; Ip; EC 1.3.5.1 from Gallus gallus (Chicken) (see 2 papers)
35% identity, 17% coverage: 22:89/405 of query aligns to 193:267/290 of Q9YHT2

query
sites
Q9YHT2

L

L

R

Y

S

E

C
|
C

V

I

H

L

C
|
C

G

A

F

C

C
|
C

N

S

A

T

T

S

C
|
C

P

P

T

S

Y

Y

Q

W

L
x
W

L

N

G

G

D

D

E

K

L

Y

D

L

G

G

P

P

R

A

G

V

R

L

I

M

Y

Q

L

A

M

Y

K

R

Q

W

M

M

F

I

E

D

G

S

-

R

G

D

E

D

V

Y

T

T

E

E

S

E

-

R

-

L

T

A

Q

Q

L

L

H

Q

-

D

-

P

-

F

-

S

L

L

D

Y

R

R

C
|
C

L

H

T

T

C

I

R

M

N

N

C
|
C

E

T

T

R

T

T

C
|
C

P

P

S

K

G

G

V

L

C196 (= C25) binding
C199 (= C28) binding
C202 (= C31) binding
C206 (= C35) binding
W211 (≠ L40) binding
C253 (= C75) binding
C259 (= C81) binding
C263 (= C85) binding

Sites not aligning to the query:

103 binding
108 binding
111 binding
123 binding

3sfdB Crystal structure of porcine mitochondrial respiratory complex ii bound with oxaloacetate and pentachlorophenol (see paper)
32% identity, 19% coverage: 12:89/405 of query aligns to 135:221/239 of 3sfdB

query
sites
3sfdB

L

L

P

Q

R

S

A

I

E

E

A

R

E

E

S

K

I

L

-

D

-

G

L

L

R

Y

S

E

C
|
C

V
x
I

H
x
L

C
|
C

G
x
A

F

C

C
|
C

N

S

A

T

T

S

C
|
C

P
|
P

T

S

Y

Y

Q

W

L
x
W

L

N

G

G

D

D

E

K

L
x
Y

D

L

G

G

P
|
P

R
x
A

G

V

R

L

I

M

Y

Q

L

A

M

Y

K

R

Q

W

M

M

F

I

E

D

G

S

-

R

G

D

E

D

V

F

T

T

E

E

-

E

-

R

-

L

-

A

-

K

-

L

S

Q

T

D

Q

P

L

F

H

S

L

L

D

Y

R

R

C
|
C

L

H

T
|
T

C
x
I

R
x
M

N
|
N

C
|
C

E

T

T

G

T

T

C
|
C

P
|
P

S
x
K

G

G

V

L

binding fe3-s4 cluster: C160 (= C35), Y170 (≠ L45), P173 (= P48), C207 (= C75), T209 (= T77), I210 (≠ C78), M211 (≠ R79), N212 (= N80), C213 (= C81)
binding pentachlorophenol: P161 (= P36), W165 (≠ L40), I210 (≠ C78)
binding iron/sulfur cluster: C150 (= C25), I151 (≠ V26), L152 (≠ H27), C153 (= C28), A154 (≠ G29), C156 (= C31), A174 (≠ R49), C217 (= C85), P218 (= P86), K219 (≠ S87)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 57, 58, 60, 62, 63, 64, 65, 77

3aegB Crystal structure of porcine heart mitochondrial complex ii bound with n-biphenyl-3-yl-2-iodo-benzamide
32% identity, 19% coverage: 12:89/405 of query aligns to 135:221/239 of 3aegB

query
sites
3aegB

L

L

P

Q

R

S

A

I

E

E

A

R

E

E

S

K

I

L

-

D

-

G

L

L

R

Y

S

E

C
|
C

V
x
I

H
x
L

C
|
C

G
x
A

F

C

C
|
C

N

S

A

T

T

S

C
|
C

P

P

T
x
S

Y

Y

Q
x
W

L
x
W

L

N

G

G

D

D

E

K

L
x
Y

D

L

G

G

P
|
P

R
x
A

G

V

R

L

I

M

Y

Q

L

A

M

Y

K

R

Q

W

M

M

F

I

E

D

G

S

-

R

G

D

E

D

V

F

T

T

E

E

-

E

-

R

-

L

-

A

-

K

-

L

S

Q

T

D

Q

P

L

F

H

S

L

L

D

Y

R

R

C
|
C

L
x
H

T

T

C
x
I

R
x
M

N

N

C
|
C

E

T

T

G

T

T

C
|
C

P

P

S

K

G

G

V

L

binding N-biphenyl-3-yl-2-iodobenzamide: S162 (≠ T37), W164 (≠ Q39), W165 (≠ L40), H208 (≠ L76)
binding fe3-s4 cluster: C160 (= C35), Y170 (≠ L45), P173 (= P48), C207 (= C75), I210 (≠ C78), M211 (≠ R79), C213 (= C81)
binding iron/sulfur cluster: C150 (= C25), I151 (≠ V26), L152 (≠ H27), C153 (= C28), A154 (≠ G29), C156 (= C31), A174 (≠ R49), C217 (= C85)

Sites not aligning to the query:

binding fe3-s4 cluster: 227
binding fe2/s2 (inorganic) cluster: 57, 58, 60, 62, 63, 65, 75, 77

3aeeB Crystal structure of porcine heart mitochondrial complex ii bound with atpenin a5
32% identity, 19% coverage: 12:89/405 of query aligns to 135:221/239 of 3aeeB

query
sites
3aeeB

L

L

P

Q

R

S

A

I

E

E

A

R

E

E

S

K

I

L

-

D

-

G

L

L

R

Y

S

E

C
|
C

V

I

H
x
L

C
|
C

G

A

F

C

C
|
C

N

S

A

T

T

S

C
|
C

P

P

T

S

Y

Y

Q

W

L
x
W

L

N

G

G

D

D

E

K

L
x
Y

D

L

G

G

P
|
P

R
x
A

G

V

R

L

I

M

Y

Q

L

A

M

Y

K

R

Q

W

M

M

F

I

E

D

G

S

-

R

G

D

E

D

V

F

T

T

E

E

-

E

-

R

-

L

-

A

-

K

-

L

S

Q

T

D

Q

P

L

F

H

S

L

L

D

Y

R

R

C
|
C

L
x
H

T
|
T

C
x
I

R
x
M

N
|
N

C
|
C

E

T

T

G

T

T

C
|
C

P
|
P

S

K

G

G

V
x
L

binding 3-[(2s,4s,5r)-5,6-dichloro-2,4-dimethyl-1-oxohexyl]-4-hydroxy-5,6-dimethoxy-2(1h)-pyridinone: W165 (≠ L40), H208 (≠ L76), I210 (≠ C78)
binding fe3-s4 cluster: C160 (= C35), Y170 (≠ L45), P173 (= P48), C207 (= C75), T209 (= T77), I210 (≠ C78), M211 (≠ R79), N212 (= N80), C213 (= C81)
binding iron/sulfur cluster: C150 (= C25), L152 (≠ H27), C153 (= C28), C156 (= C31), A174 (≠ R49), C217 (= C85), P218 (= P86), L221 (≠ V89)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 57, 58, 60, 62, 63, 65, 77

3aedB Crystal structure of porcine heart mitochondrial complex ii bound with 2-iodo-n-phenyl-benzamide
32% identity, 19% coverage: 12:89/405 of query aligns to 135:221/239 of 3aedB

query
sites
3aedB

L

L

P

Q

R

S

A

I

E

E

A

R

E

E

S

K

I

L

-

D

-

G

L

L

R

Y

S

E

C
|
C

V
x
I

H
x
L

C
|
C

G
x
A

F

C

C
|
C

N

S

A

T

T

S

C
|
C

P
|
P

T

S

Y

Y

Q

W

L
x
W

L

N

G

G

D

D

E

K

L
x
Y

D

L

G

G

P
|
P

R

A

G

V

R

L

I

M

Y

Q

L

A

M

Y

K

R

Q

W

M

M

F

I

E

D

G

S

-

R

G

D

E

D

V

F

T

T

E

E

-

E

-

R

-

L

-

A

-

K

-

L

S

Q

T

D

Q

P

L

F

H

S

L

L

D

Y

R

R

C
|
C

L
x
H

T

T

C
x
I

R
x
M

N
|
N

C
|
C

E

T

T

G

T

T

C
|
C

P

P

S

K

G

G

V
x
L

binding 2-iodo-N-phenylbenzamide: P161 (= P36), W165 (≠ L40), H208 (≠ L76), I210 (≠ C78)
binding fe3-s4 cluster: C160 (= C35), Y170 (≠ L45), P173 (= P48), C207 (= C75), I210 (≠ C78), M211 (≠ R79), N212 (= N80), C213 (= C81)
binding iron/sulfur cluster: C150 (= C25), I151 (≠ V26), L152 (≠ H27), C153 (= C28), A154 (≠ G29), C156 (= C31), C217 (= C85), L221 (≠ V89)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 57, 58, 60, 62, 63, 65, 77

3aecB Crystal structure of porcine heart mitochondrial complex ii bound with 2-iodo-n-(1-methylethyl)-benzamid
32% identity, 19% coverage: 12:89/405 of query aligns to 135:221/239 of 3aecB

query
sites
3aecB

L

L

P

Q

R

S

A

I

E

E

A

R

E

E

S

K

I

L

-

D

-

G

L

L

R

Y

S

E

C
|
C

V
x
I

H
x
L

C
|
C

G

A

F

C

C
|
C

N

S

A

T

T

S

C
|
C

P
|
P

T

S

Y

Y

Q

W

L
x
W

L

N

G

G

D

D

E

K

L
x
Y

D

L

G

G

P
|
P

R
x
A

G

V

R

L

I

M

Y

Q

L

A

M

Y

K

R

Q

W

M

M

F

I

E

D

G

S

-

R

G

D

E

D

V

F

T

T

E

E

-

E

-

R

-

L

-

A

-

K

-

L

S

Q

T

D

Q

P

L

F

H

S

L

L

D

Y

R

R

C
|
C

L
x
H

T

T

C
x
I

R
x
M

N
|
N

C
|
C

E

T

T

G

T

T

C
|
C

P
|
P

S

K

G

G

V
x
L

binding 2-iodo-N-(1-methylethyl)benzamide: P161 (= P36), W165 (≠ L40), H208 (≠ L76)
binding fe3-s4 cluster: C160 (= C35), Y170 (≠ L45), P173 (= P48), C207 (= C75), I210 (≠ C78), M211 (≠ R79), N212 (= N80), C213 (= C81)
binding iron/sulfur cluster: C150 (= C25), I151 (≠ V26), L152 (≠ H27), C153 (= C28), C156 (= C31), A174 (≠ R49), C217 (= C85), P218 (= P86), L221 (≠ V89)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 57, 58, 62, 63, 65, 75, 77

3aebB Crystal structure of porcine heart mitochondrial complex ii bound with n-(3-phenoxy-phenyl)-2-trifluoromethyl-benzamide
32% identity, 19% coverage: 12:89/405 of query aligns to 135:221/239 of 3aebB

query
sites
3aebB

L

L

P

Q

R

S

A

I

E

E

A

R

E

E

S

K

I

L

-

D

-

G

L

L

R

Y

S

E

C
|
C

V
x
I

H
x
L

C
|
C

G
x
A

F

C

C
|
C

N

S

A

T

T

S

C
|
C

P

P

T

S

Y

Y

Q

W

L
x
W

L

N

G

G

D

D

E

K

L
x
Y

D

L

G

G

P
|
P

R
x
A

G

V

R

L

I

M

Y

Q

L

A

M

Y

K

R

Q

W

M

M

F

I

E

D

G

S

-

R

G

D

E

D

V

F

T

T

E

E

-

E

-

R

-

L

-

A

-

K

-

L

S

Q

T

D

Q

P

L

F

H

S

L

L

D

Y

R

R

C
|
C

L
x
H

T
|
T

C
x
I

R
x
M

N
|
N

C
|
C

E

T

T

G

T

T

C
|
C

P

P

S

K

G

G

V
x
L

binding fe3-s4 cluster: C160 (= C35), Y170 (≠ L45), P173 (= P48), C207 (= C75), T209 (= T77), I210 (≠ C78), M211 (≠ R79), N212 (= N80), C213 (= C81)
binding N-(3-phenoxyphenyl)-2-(trifluoromethyl)benzamide: W165 (≠ L40), H208 (≠ L76)
binding iron/sulfur cluster: C150 (= C25), I151 (≠ V26), L152 (≠ H27), C153 (= C28), A154 (≠ G29), C156 (= C31), A174 (≠ R49), C217 (= C85), L221 (≠ V89)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 57, 58, 62, 63, 65, 77

3aeaB Crystal structure of porcine heart mitochondrial complex ii bound with n-(3-dimethylaminomethyl-phenyl)-2-trifluoromethyl-benzamide (see paper)
32% identity, 19% coverage: 12:89/405 of query aligns to 135:221/239 of 3aeaB

query
sites
3aeaB

L

L

P

Q

R

S

A

I

E

E

A

R

E

E

S

K

I

L

-

D

-

G

L

L

R

Y

S

E

C
|
C

V
x
I

H
x
L

C
|
C

G

A

F

C

C
|
C

N

S

A

T

T

S

C
|
C

P
|
P

T

S

Y

Y

Q

W

L
x
W

L

N

G

G

D

D

E

K

L
x
Y

D

L

G

G

P
|
P

R
x
A

G

V

R

L

I

M

Y

Q

L

A

M

Y

K

R

Q

W

M

M

F

I

E

D

G

S

-

R

G

D

E

D

V

F

T

T

E

E

-

E

-

R

-

L

-

A

-

K

-

L

S

Q

T

D

Q

P

L

F

H

S

L

L

D

Y

R

R

C
|
C

L
x
H

T
|
T

C
x
I

R
x
M

N
|
N

C
|
C

E

T

T

G

T

T

C
|
C

P
|
P

S

K

G

G

V
x
L

binding fe3-s4 cluster: C160 (= C35), Y170 (≠ L45), P173 (= P48), C207 (= C75), T209 (= T77), I210 (≠ C78), M211 (≠ R79), N212 (= N80), C213 (= C81)
binding N-{3-[(dimethylamino)methyl]phenyl}-2-(trifluoromethyl)benzamide: P161 (= P36), W165 (≠ L40), H208 (≠ L76), I210 (≠ C78)
binding iron/sulfur cluster: C150 (= C25), I151 (≠ V26), L152 (≠ H27), C153 (= C28), C156 (= C31), A174 (≠ R49), C217 (= C85), P218 (= P86), L221 (≠ V89)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 57, 58, 60, 62, 63, 65, 77

3ae9B Crystal structure of porcine heart mitochondrial complex ii bound with n-(3-pentafluorophenyloxy-phenyl)-2-trifluoromethyl-benzamide (see paper)
32% identity, 19% coverage: 12:89/405 of query aligns to 135:221/239 of 3ae9B

query
sites
3ae9B

L

L

P

Q

R

S

A

I

E

E

A

R

E

E

S

K

I

L

-

D

-

G

L

L

R

Y

S

E

C
|
C

V
x
I

H
x
L

C
|
C

G
x
A

F

C

C
|
C

N

S

A

T

T

S

C
|
C

P
|
P

T

S

Y

Y

Q

W

L
x
W

L

N

G

G

D

D

E

K

L
x
Y

D

L

G

G

P
|
P

R
x
A

G

V

R

L

I

M

Y

Q

L

A

M

Y

K

R

Q

W

M

M

F

I

E

D

G

S

-

R

G

D

E

D

V

F

T

T

E

E

-

E

-

R

-

L

-

A

-

K

-

L

S

Q

T

D

Q

P

L

F

H

S

L

L

D

Y

R

R

C
|
C

L
x
H

T
|
T

C
x
I

R
x
M

N
|
N

C
|
C

E

T

T

G

T

T

C
|
C

P

P

S

K

G

G

V
x
L

binding fe3-s4 cluster: C160 (= C35), Y170 (≠ L45), P173 (= P48), C207 (= C75), H208 (≠ L76), T209 (= T77), I210 (≠ C78), M211 (≠ R79), N212 (= N80), C213 (= C81)
binding N-[3-(pentafluorophenoxy)phenyl]-2-(trifluoromethyl)benzamide: P161 (= P36), W165 (≠ L40), H208 (≠ L76)
binding iron/sulfur cluster: C150 (= C25), I151 (≠ V26), L152 (≠ H27), C153 (= C28), A154 (≠ G29), C156 (= C31), A174 (≠ R49), C217 (= C85), L221 (≠ V89)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 57, 58, 60, 62, 63, 65, 77

3ae8B Crystal structure of porcine heart mitochondrial complex ii bound with n-(3-isopropoxy-phenyl)-2-trifluoromethylbenzamide
32% identity, 19% coverage: 12:89/405 of query aligns to 135:221/239 of 3ae8B

query
sites
3ae8B

L

L

P

Q

R

S

A

I

E

E

A

R

E

E

S

K

I

L

-

D

-

G

L

L

R

Y

S

E

C
|
C

V
x
I

H
x
L

C
|
C

G

A

F

C

C
|
C

N

S

A

T

T

S

C
|
C

P

P

T
x
S

Y

Y

Q

W

L
x
W

L

N

G

G

D

D

E

K

L

Y

D

L

G

G

P
|
P

R
x
A

G

V

R

L

I

M

Y

Q

L

A

M

Y

K

R

Q

W

M

M

F

I

E

D

G

S

-

R

G

D

E

D

V

F

T

T

E

E

-

E

-

R

-

L

-

A

-

K

-

L

S

Q

T

D

Q

P

L

F

H

S

L

L

D

Y

R

R

C
|
C

L
x
H

T
|
T

C
x
I

R
x
M

N

N

C
|
C

E

T

T

G

T

T

C
|
C

P
|
P

S

K

G

G

V
x
L

binding fe3-s4 cluster: C160 (= C35), P173 (= P48), C207 (= C75), T209 (= T77), I210 (≠ C78), M211 (≠ R79), C213 (= C81)
binding N-[3-(1-methylethoxy)phenyl]-2-(trifluoromethyl)benzamide: S162 (≠ T37), W165 (≠ L40), H208 (≠ L76)
binding iron/sulfur cluster: C150 (= C25), I151 (≠ V26), L152 (≠ H27), C153 (= C28), C156 (= C31), A174 (≠ R49), C217 (= C85), P218 (= P86), L221 (≠ V89)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 57, 58, 62, 63, 65, 75, 77

Query Sequence

>GFF3770 FitnessBrowser__psRCH2:GFF3770
MQTNLSEAAKKLPRAEEAESILRSCVHCGFCNATCPTYQLLGDELDGPRGRIYLMKQMFE
GGEVTESTQLHLDRCLTCRNCETTCPSGVKYHNLLDIGRDFIEQQVQRPLGERVVRGGLR
TVIPRPGLFKALLGAGNALKPLMPASLKDHLPREIRPAKPRPQVMHSRRVLILEGCVQPS
LSPSTNAAAARVLDRLGISVSPAREAGCCGAVDYHLNAQDAGLDRARRNIDAWWPAIEAG
AEAIVQTASGCGAFVKEYGHLLKDDPAYAAKAARVSELAKDLVEVLRSAELEKLNVRADK
RMAFHCPCTLQHAQKLGGAVEDVLTRLGYQLTAVPDAHLCCGSAGSYSITQPEISHQLRD
NKLNALESGKPEVIVTANIGCQTHLDGAGRTPVKHWIEVVEESMQ

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory