SitesBLAST
Comparing GFF3794 FitnessBrowser__Phaeo:GFF3794 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
5zaiC Crystal structure of 3-hydroxypropionyl-coa dehydratase from metallosphaera sedula (see paper)
41% identity, 99% coverage: 3:262/263 of query aligns to 4:258/259 of 5zaiC
- active site: A65 (≠ G65), F70 (= F70), S82 (≠ C86), R86 (≠ Q90), G110 (= G114), E113 (= E117), P132 (= P136), E133 (= E137), I138 (= I142), P140 (= P144), G141 (≠ T145), A226 (≠ L230), F236 (≠ A240)
- binding coenzyme a: K24 (= K23), L25 (= L24), A63 (= A63), G64 (= G64), A65 (≠ G65), D66 (= D66), I67 (= I67), P132 (= P136), R166 (≠ T170), F248 (≠ W252), K251 (≠ R255)
3q0jC Crystal structure of the mycobacterium tuberculosis crotonase in complex with the inhibitor acetoacetylcoa
41% identity, 98% coverage: 3:260/263 of query aligns to 4:254/255 of 3q0jC
- active site: A65 (≠ G65), M70 (≠ F70), T80 (≠ V82), F84 (≠ C86), G108 (= G114), E111 (= E117), P130 (= P136), E131 (= E137), V136 (≠ I142), P138 (= P144), G139 (≠ T145), L224 (= L230), F234 (≠ A240)
- binding acetoacetyl-coenzyme a: Q23 (≠ E22), A24 (≠ K23), L25 (= L24), A27 (= A26), A63 (= A63), G64 (= G64), A65 (≠ G65), D66 (= D66), I67 (= I67), K68 (≠ H68), M70 (≠ F70), F84 (≠ C86), G107 (= G113), G108 (= G114), E111 (= E117), P130 (= P136), E131 (= E137), P138 (= P144), G139 (≠ T145), M140 (≠ F146)
3q0gC Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
41% identity, 98% coverage: 3:260/263 of query aligns to 4:254/255 of 3q0gC
- active site: A65 (≠ G65), M70 (≠ F70), T80 (≠ V82), F84 (≠ C86), G108 (= G114), E111 (= E117), P130 (= P136), E131 (= E137), V136 (≠ I142), P138 (= P144), G139 (≠ T145), L224 (= L230), F234 (≠ A240)
- binding coenzyme a: L25 (= L24), A63 (= A63), I67 (= I67), K68 (≠ H68), Y104 (≠ I110), P130 (= P136), E131 (= E137), L134 (≠ I140)
3h81A Crystal structure of enoyl-coa hydratase from mycobacterium tuberculosis (see paper)
41% identity, 98% coverage: 3:261/263 of query aligns to 3:254/256 of 3h81A
- active site: A64 (≠ G65), M69 (≠ F70), T79 (≠ V82), F83 (≠ C86), G107 (= G114), E110 (= E117), P129 (= P136), E130 (= E137), V135 (≠ I142), P137 (= P144), G138 (≠ T145), L223 (= L230), F233 (≠ A240)
- binding calcium ion: F233 (≠ A240), Q238 (≠ V245)
3q0gD Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
41% identity, 98% coverage: 3:260/263 of query aligns to 3:249/250 of 3q0gD
- active site: A64 (≠ G65), M69 (vs. gap), T75 (≠ K75), F79 (= F85), G103 (= G114), E106 (= E117), P125 (= P136), E126 (= E137), V131 (≠ I142), P133 (= P144), G134 (≠ T145), L219 (= L230), F229 (≠ A240)
- binding Butyryl Coenzyme A: F225 (= F236), F241 (≠ W252)
2hw5C The crystal structure of human enoyl-coenzyme a (coa) hydratase short chain 1, echs1
40% identity, 96% coverage: 9:260/263 of query aligns to 13:257/260 of 2hw5C
- active site: A68 (≠ G65), M73 (≠ F70), S83 (≠ V82), L87 (≠ C86), G111 (= G114), E114 (= E117), P133 (= P136), E134 (= E137), T139 (≠ I142), P141 (= P144), G142 (≠ T145), K227 (≠ L230), F237 (≠ A240)
- binding crotonyl coenzyme a: K26 (≠ E22), A27 (≠ K23), L28 (= L24), A30 (= A26), K62 (≠ R59), I70 (= I67), F109 (= F112)
1mj3A Crystal structure analysis of rat enoyl-coa hydratase in complex with hexadienoyl-coa (see paper)
38% identity, 96% coverage: 9:260/263 of query aligns to 13:255/258 of 1mj3A
- active site: A68 (≠ G65), M73 (≠ F70), S83 (≠ R88), L85 (≠ Q90), G109 (= G114), E112 (= E117), P131 (= P136), E132 (= E137), T137 (≠ I142), P139 (= P144), G140 (≠ T145), K225 (≠ L230), F235 (≠ A240)
- binding hexanoyl-coenzyme a: K26 (≠ E22), A27 (≠ K23), L28 (= L24), A30 (= A26), A66 (= A63), G67 (= G64), A68 (≠ G65), D69 (= D66), I70 (= I67), G109 (= G114), P131 (= P136), E132 (= E137), L135 (≠ I140), G140 (≠ T145)
1ey3A Structure of enoyl-coa hydratase complexed with the substrate dac-coa (see paper)
38% identity, 96% coverage: 9:260/263 of query aligns to 11:255/258 of 1ey3A
- active site: A66 (≠ G65), M71 (≠ F70), S81 (≠ R88), L85 (≠ M92), G109 (= G114), E112 (= E117), P131 (= P136), E132 (= E137), T137 (≠ I142), P139 (= P144), G140 (≠ T145), K225 (≠ L230), F235 (≠ A240)
- binding 4-(n,n-dimethylamino)cinnamoyl-coa: K24 (≠ E22), L26 (= L24), A28 (= A26), A64 (= A63), G65 (= G64), A66 (≠ G65), D67 (= D66), I68 (= I67), L85 (≠ M92), W88 (vs. gap), G109 (= G114), P131 (= P136), L135 (≠ I140), G140 (≠ T145)
1dubA 2-enoyl-coa hydratase, data collected at 100 k, ph 6.5 (see paper)
38% identity, 96% coverage: 9:260/263 of query aligns to 13:257/260 of 1dubA
- active site: A68 (≠ G65), M73 (≠ F70), S83 (≠ R88), L87 (≠ M92), G111 (= G114), E114 (= E117), P133 (= P136), E134 (= E137), T139 (≠ I142), P141 (= P144), G142 (≠ T145), K227 (≠ L230), F237 (≠ A240)
- binding acetoacetyl-coenzyme a: K26 (≠ E22), A27 (≠ K23), L28 (= L24), A30 (= A26), A66 (= A63), A68 (≠ G65), D69 (= D66), I70 (= I67), Y107 (≠ I110), G110 (= G113), G111 (= G114), E114 (= E117), P133 (= P136), E134 (= E137), L137 (≠ I140), G142 (≠ T145), F233 (= F236), F249 (≠ W252)
P14604 Enoyl-CoA hydratase, mitochondrial; mECH; mECH1; Enoyl-CoA hydratase 1; ECHS1; Short-chain enoyl-CoA hydratase; SCEH; EC 4.2.1.17; EC 5.3.3.8 from Rattus norvegicus (Rat) (see 3 papers)
38% identity, 96% coverage: 9:260/263 of query aligns to 43:287/290 of P14604
- E144 (= E117) mutation to D: Reduces activity 50-fold.; mutation to Q: Reduces activity 3300-fold.
- E164 (= E137) mutation to D: Reduces activity 1250-fold.; mutation to Q: Reduces activity 330000-fold.
Sites not aligning to the query:
- 1:29 modified: transit peptide, Mitochondrion
2dubA Enoyl-coa hydratase complexed with octanoyl-coa (see paper)
38% identity, 96% coverage: 9:260/263 of query aligns to 12:251/254 of 2dubA
- active site: A67 (≠ G65), M72 (≠ F70), S82 (≠ R88), G105 (= G114), E108 (= E117), P127 (= P136), E128 (= E137), T133 (≠ I142), P135 (= P144), G136 (≠ T145), K221 (≠ L230), F231 (≠ A240)
- binding octanoyl-coenzyme a: K25 (≠ E22), A26 (≠ K23), L27 (= L24), A29 (= A26), A65 (= A63), A67 (≠ G65), D68 (= D66), I69 (= I67), K70 (≠ H68), G105 (= G114), E108 (= E117), P127 (= P136), E128 (= E137), G136 (≠ T145), A137 (≠ F146)
Q4WF54 Mevalonyl-coenzyme A hydratase sidH; Siderophore biosynthesis protein H; EC 4.2.1.- from Aspergillus fumigatus (strain ATCC MYA-4609 / CBS 101355 / FGSC A1100 / Af293) (Neosartorya fumigata) (see paper)
35% identity, 94% coverage: 13:260/263 of query aligns to 21:265/270 of Q4WF54
Sites not aligning to the query:
- 268:270 PTS1-type peroxisomal targeting signal
5jbxB Crystal structure of liuc in complex with coenzyme a and malonic acid (see paper)
33% identity, 96% coverage: 11:262/263 of query aligns to 13:260/261 of 5jbxB
- active site: A67 (≠ G65), R72 (≠ F70), L84 (≠ C86), R88 (≠ Q90), G112 (= G114), E115 (= E117), T134 (≠ P136), E135 (= E137), I140 (= I142), P142 (= P144), G143 (≠ T145), A228 (≠ L230), L238 (≠ A240)
- binding coenzyme a: S24 (≠ E22), R25 (≠ K23), R26 (≠ L24), A28 (= A26), A65 (= A63), D68 (= D66), L69 (≠ I67), K70 (≠ H68), L110 (≠ F112), G111 (= G113), T134 (≠ P136), E135 (= E137), L138 (≠ I140), R168 (≠ T170)
6slbAAA Enoyl-CoA hydratase/carnithine racemase (see paper)
36% identity, 98% coverage: 4:262/263 of query aligns to 4:256/257 of 6slbAAA
- active site: Q64 (≠ G65), F69 (= F70), L80 (≠ V82), N84 (≠ C86), A108 (≠ G114), S111 (≠ E117), A130 (≠ P136), F131 (≠ E137), L136 (≠ I142), P138 (= P144), D139 (≠ T145), A224 (≠ L230), G234 (≠ A240)
- binding (~{E})-6-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethylsulfanyl]-6-oxidanylidene-hex-3-enoic acid: R58 (= R59), A62 (= A63), Q64 (≠ G65), D65 (= D66), L66 (≠ I67), Y76 (≠ V78), A108 (≠ G114), F131 (≠ E137), D139 (≠ T145)
6slaAAA Enoyl-CoA hydratase/carnithine racemase (see paper)
35% identity, 98% coverage: 4:262/263 of query aligns to 1:244/245 of 6slaAAA
- active site: Q61 (≠ G65), L68 (≠ C86), N72 (≠ Q90), A96 (≠ G114), S99 (≠ E117), A118 (≠ P136), F119 (≠ E137), L124 (≠ I142), P126 (= P144), N127 (≠ T145), A212 (≠ L230), G222 (≠ A240)
- binding ~{S}-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethyl] 2-(2,5-dihydrooxepin-7-yl)ethanethioate: L21 (= L24), A59 (= A63), Q61 (≠ G65), D62 (= D66), L63 (≠ I67), L68 (≠ C86), Y71 (≠ G89), A94 (≠ F112), G95 (= G113), A96 (≠ G114), F119 (≠ E137), I122 (= I140), L124 (≠ I142), N127 (≠ T145), F234 (≠ W252), K237 (≠ R255)
2vssD Wild-type hydroxycinnamoyl-coa hydratase lyase in complex with acetyl- coa and vanillin (see paper)
37% identity, 78% coverage: 5:208/263 of query aligns to 9:212/246 of 2vssD