SitesBLAST
Comparing GFF3809 FitnessBrowser__WCS417:GFF3809 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
6rl5G The first crystal structure of the daba aminotransferase ectb in the ectoine biosynthesis pathway of the model organism chromohalobacter salexigens dsm 3034 (see paper)
37% identity, 94% coverage: 22:456/464 of query aligns to 1:422/422 of 6rl5G
- active site: S16 (≠ P40), F137 (≠ Y161), D237 (= D270), K266 (= K299)
- binding pyridoxal-5'-phosphate: T109 (= T133), G110 (= G134), T111 (= T135), F137 (≠ Y161), H138 (= H162), G139 (= G163), E204 (= E237), D237 (= D270), I239 (= I272), Q240 (= Q273), K266 (= K299), G294 (= G326), T295 (= T327), F296 (= F328)
1wkhA Acetylornithine aminotransferase from thermus thermophilus hb8
38% identity, 89% coverage: 45:458/464 of query aligns to 21:387/387 of 1wkhA
- active site: F132 (≠ Y161), E184 (= E237), D217 (= D270), Q220 (= Q273), K246 (= K299), T275 (= T327), R363 (= R434)
- binding 4-[(1,3-dicarboxy-propylamino)-methyl]-3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridinium: Y46 (≠ A70), S104 (≠ T133), G105 (= G134), T106 (= T135), N109 (≠ V138), F132 (≠ Y161), S133 (≠ H162), G134 (= G163), E184 (= E237), E189 (= E242), D217 (= D270), I219 (= I272), Q220 (= Q273), K246 (= K299), T274 (≠ G326), T275 (= T327), R363 (= R434)
Sites not aligning to the query:
1wkgA Acetylornithine aminotransferase from thermus thermophilus hb8
38% identity, 89% coverage: 45:458/464 of query aligns to 21:387/387 of 1wkgA
- active site: F132 (≠ Y161), E184 (= E237), D217 (= D270), Q220 (= Q273), K246 (= K299), T275 (= T327), R363 (= R434)
- binding n~2~-acetyl-n~5~-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-l-ornithine: Y46 (≠ A70), S104 (≠ T133), G105 (= G134), T106 (= T135), N109 (≠ V138), F132 (≠ Y161), S133 (≠ H162), G134 (= G163), R135 (≠ M164), E184 (= E237), E189 (= E242), D217 (= D270), I219 (= I272), Q220 (= Q273), K246 (= K299), G273 (≠ A325), T274 (≠ G326), T275 (= T327)
Sites not aligning to the query:
1vefA Acetylornithine aminotransferase from thermus thermophilus hb8
38% identity, 89% coverage: 45:458/464 of query aligns to 21:387/387 of 1vefA
- active site: F132 (≠ Y161), D217 (= D270), K246 (= K299), T275 (= T327), R363 (= R434)
- binding pyridoxal-5'-phosphate: S104 (≠ T133), G105 (= G134), T106 (= T135), N109 (≠ V138), F132 (≠ Y161), S133 (≠ H162), G134 (= G163), E184 (= E237), D217 (= D270), I219 (= I272), Q220 (= Q273), K246 (= K299), T274 (≠ G326), T275 (= T327)
Sites not aligning to the query:
Q5SHH5 [LysW]-aminoadipate semialdehyde transaminase; EC 2.6.1.118 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)
38% identity, 89% coverage: 45:458/464 of query aligns to 29:395/395 of Q5SHH5
- GT 113:114 (= GT 134:135) binding
- K254 (= K299) modified: N6-(pyridoxal phosphate)lysine
- T283 (= T327) binding
7vo1A Structure of aminotransferase-substrate complex (see paper)
33% identity, 90% coverage: 40:457/464 of query aligns to 31:440/452 of 7vo1A
- binding N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-L-glutamic acid: I61 (≠ A70), S121 (≠ T133), G122 (= G134), T123 (= T135), F149 (≠ Y161), H150 (= H162), G151 (= G163), R152 (≠ M164), E229 (= E237), E234 (= E242), D262 (= D270), V264 (≠ I272), Q265 (= Q273), K291 (= K299), S317 (≠ A325), N318 (≠ G326), T319 (= T327), F320 (= F328), R417 (= R434)
7vntA Structure of aminotransferase-substrate complex (see paper)
33% identity, 90% coverage: 40:457/464 of query aligns to 31:440/452 of 7vntA
- binding L-ornithine: I61 (≠ A70), F149 (≠ Y161), R152 (≠ M164), E234 (= E242), K291 (= K299)
- binding pyridoxal-5'-phosphate: S121 (≠ T133), G122 (= G134), T123 (= T135), N126 (≠ V138), F149 (≠ Y161), H150 (= H162), G151 (= G163), E229 (= E237), D262 (= D270), V264 (≠ I272), Q265 (= Q273), K291 (= K299), N318 (≠ G326), T319 (= T327)
Sites not aligning to the query:
7vnoA Structure of aminotransferase (see paper)
33% identity, 90% coverage: 40:457/464 of query aligns to 31:440/452 of 7vnoA
- binding pyridoxal-5'-phosphate: S121 (≠ T133), G122 (= G134), T123 (= T135), N126 (≠ V138), F149 (≠ Y161), H150 (= H162), G151 (= G163), E229 (= E237), D262 (= D270), V264 (≠ I272), Q265 (= Q273), K291 (= K299), N318 (≠ G326), T319 (= T327), F320 (= F328)
P42588 Putrescine aminotransferase; PAT; PATase; Cadaverine transaminase; Diamine transaminase; Putrescine transaminase; Putrescine--2-oxoglutaric acid transaminase; Putrescine:2-OG aminotransferase; EC 2.6.1.82; EC 2.6.1.29 from Escherichia coli (strain K12) (see paper)
32% identity, 88% coverage: 57:464/464 of query aligns to 78:456/459 of P42588
- GT 150:151 (= GT 134:135) binding in other chain
- Q274 (= Q273) binding in other chain
- K300 (= K299) modified: N6-(pyridoxal phosphate)lysine
- T332 (= T327) binding
4uoxC Crystal structure of ygjg in complex with pyridoxal-5'-phosphate and putrescine (see paper)
32% identity, 88% coverage: 57:464/464 of query aligns to 76:454/456 of 4uoxC
- active site: F178 (≠ Y161), E236 (= E237), D269 (= D270), Q272 (= Q273), K298 (= K299), T330 (= T327), R424 (= R434)
- binding pyridoxal-5'-phosphate: S147 (≠ T133), G148 (= G134), T149 (= T135), F178 (≠ Y161), H179 (= H162), G180 (= G163), E236 (= E237), E241 (= E242), D269 (= D270), V271 (≠ I272), Q272 (= Q273), K298 (= K299), T329 (≠ G326), T330 (= T327)
- binding 1,4-diaminobutane: F89 (≠ A70), K298 (= K299)
Sites not aligning to the query:
P50457 4-aminobutyrate aminotransferase PuuE; GABA aminotransferase; GABA-AT; Gamma-amino-N-butyrate transaminase; GABA transaminase; Glutamate:succinic semialdehyde transaminase; EC 2.6.1.19 from Escherichia coli (strain K12) (see paper)
34% identity, 93% coverage: 29:458/464 of query aligns to 8:420/421 of P50457