SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF3828 FitnessBrowser__Phaeo:GFF3828 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

6lpiB Crystal structure of ahas holo-enzyme (see paper)
31% identity, 84% coverage: 10:473/551 of query aligns to 7:456/539 of 6lpiB

query
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6lpiB

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active site: I27 (= I30), G29 (= G32), G30 (≠ V33), S31 (≠ H34), I32 (≠ T35), E53 (= E55), C76 (≠ I78), F115 (≠ L119), Q116 (≠ H120), E117 (= E121), K165 (≠ L173), M256 (≠ S264), A283 (= A294), V375 (≠ S391), G401 (= G418), M403 (≠ L420), D428 (= D445), N455 (= N472)
binding flavin-adenine dinucleotide: R155 (= R163), G212 (= G221), G213 (= G222), G214 (= G223), T236 (= T245), L237 (≠ V246), M238 (≠ N247), M253 (≠ A261), L254 (≠ S262), G255 (≠ P263), M256 (≠ S264), H257 (≠ L265), G276 (= G282), A277 (≠ T283), R278 (≠ E284), D280 (= D291), R282 (≠ Y293), A283 (= A294), D300 (= D308), I301 (≠ L309), D302 (≠ C310), E305 (≠ Q313), A318 (≠ G325), D319 (= D326), V320 (= V327), Q379 (≠ I395), M380 (≠ Y396), G398 (≠ A414), G399 (= G416)
binding magnesium ion: D428 (= D445), N455 (= N472)
binding thiamine diphosphate: P28 (= P31), E53 (= E55), C76 (≠ I78), P79 (= P81), G80 (= G82), N83 (= N85), V375 (≠ S391), G376 (≠ A392), Q377 (= Q393), H378 (≠ P394), G401 (= G418), T402 (≠ A419), M403 (≠ L420), G427 (= G444), D428 (= D445), G429 (= G446), S430 (≠ G447), M433 (≠ F450), N455 (= N472)

Sites not aligning to the query:

active site: 457, 458, 460, 461, 464
binding thiamine diphosphate: 457, 458, 459, 460, 461

7tzzA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase p197t mutant in complex with bispyribac-sodium (see paper)
29% identity, 85% coverage: 13:483/551 of query aligns to 16:488/582 of 7tzzA

query
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7tzzA

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binding 2,6-bis[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid: M266 (≠ S264), D291 (≠ M292), R292 (≠ Y293), M485 (≠ R480)
binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: V400 (≠ D390), G401 (≠ S391), Q402 (≠ A392), H403 (≠ Q393), G426 (= G418), A427 (= A419), M428 (≠ L420), G452 (= G444), D453 (= D445), G454 (= G446), S455 (≠ G447), M458 (≠ F450), N480 (= N472), H482 (≠ E477), L483 (≠ I478), G484 (≠ A479), M485 (≠ R480), V486 (≠ S481)
binding flavin-adenine dinucleotide: R161 (= R163), G222 (= G221), G223 (= G222), G224 (= G223), T246 (= T245), L247 (≠ V246), M248 (≠ N247), M263 (≠ A261), L264 (≠ S262), G265 (≠ P263), M266 (≠ S264), H267 (≠ L265), G286 (= G282), V287 (≠ T283), R288 (≠ E284), D290 (= D291), R292 (≠ Y293), V293 (≠ A294), I309 (= I307), D310 (= D308), I311 (≠ L309), D312 (≠ C310), E315 (≠ Q313), G328 (= G325), D329 (= D326), V330 (= V327), Q404 (≠ P394), M405 (≠ I395), G423 (≠ A414), G424 (= G416)
binding magnesium ion: G36 (≠ V33), A37 (≠ H34), T82 (≠ I78), S83 (≠ T79), Q122 (≠ H120), F370 (≠ W361), Y381 (vs. gap), D453 (= D445), M458 (≠ F450), Q461 (≠ P453), N480 (= N472), H482 (≠ E477)

Sites not aligning to the query:

8et4A Crystal structure of wild-type arabidopsis thaliana acetohydroxyacid synthase in complex with amidosulfuron (see paper)
29% identity, 85% coverage: 13:483/551 of query aligns to 16:488/582 of 8et4A

query
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8et4A

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M

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A

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Q

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E

A

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Q

binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: V400 (≠ D390), G401 (≠ S391), Q402 (≠ A392), H403 (≠ Q393), G426 (= G418), A427 (= A419), M428 (≠ L420), G452 (= G444), D453 (= D445), G454 (= G446), S455 (≠ G447), M458 (≠ F450), N480 (= N472), H482 (≠ E477), L483 (≠ I478), G484 (≠ A479), M485 (≠ R480), V486 (≠ S481)
binding flavin-adenine dinucleotide: R161 (= R163), G222 (= G221), G223 (= G222), G224 (= G223), T246 (= T245), L247 (≠ V246), M248 (≠ N247), M263 (≠ A261), L264 (≠ S262), G265 (≠ P263), M266 (≠ S264), H267 (≠ L265), G286 (= G282), V287 (≠ T283), R288 (≠ E284), D290 (= D291), R292 (≠ Y293), V293 (≠ A294), D310 (= D308), I311 (≠ L309), D312 (≠ C310), E315 (≠ Q313), G328 (= G325), D329 (= D326), V330 (= V327), Q404 (≠ P394), M405 (≠ I395), G423 (≠ A414), G424 (= G416)
binding magnesium ion: F370 (≠ W361), Y381 (vs. gap), D453 (= D445), M458 (≠ F450), Q461 (≠ P453), N480 (= N472), H482 (≠ E477)
binding N-{[(4,6-dimethoxypyrimidin-2-yl)carbamoyl]sulfamoyl}-N-methylmethanesulfonamide: M266 (≠ S264), H267 (≠ L265), R292 (≠ Y293), M485 (≠ R480), V486 (≠ S481)

Sites not aligning to the query:

5wj1A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a triazolopyrimidine herbicide, penoxsulam (see paper)
29% identity, 85% coverage: 13:483/551 of query aligns to 16:488/582 of 5wj1A

query
sites
5wj1A

E

D

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E

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E

A

N

L

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N

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active site: Y33 (≠ I30), G35 (= G32), G36 (≠ V33), A37 (≠ H34), S38 (≠ T35), E59 (= E55), T82 (≠ I78), F121 (≠ L119), Q122 (≠ H120), E123 (= E121), K171 (≠ L173), M266 (≠ S264), V293 (≠ A294), V400 (≠ D390), G426 (= G418), M428 (≠ L420), D453 (= D445), N480 (= N472), H482 (≠ E477), L483 (≠ I478), M485 (≠ R480), V486 (≠ S481)
binding flavin-adenine dinucleotide: R161 (= R163), G222 (= G221), G223 (= G222), G224 (= G223), T246 (= T245), L247 (≠ V246), M248 (≠ N247), M263 (≠ A261), L264 (≠ S262), G265 (≠ P263), M266 (≠ S264), H267 (≠ L265), G286 (= G282), V287 (≠ T283), R288 (≠ E284), D290 (= D291), R292 (≠ Y293), V293 (≠ A294), I309 (= I307), D310 (= D308), I311 (≠ L309), D312 (≠ C310), E315 (≠ Q313), G328 (= G325), D329 (= D326), V330 (= V327), Q404 (≠ P394), M405 (≠ I395), G423 (≠ A414), G424 (= G416), M485 (≠ R480)
binding magnesium ion: D453 (= D445), N480 (= N472), H482 (≠ E477)
binding 2-(2,2-difluoroethoxy)-N-(5,8-dimethoxy[1,2,4]triazolo[1,5-c]pyrimidin-2-yl)-6-(trifluoromethyl)benzenesulfonamide: M266 (≠ S264), D291 (≠ M292), R292 (≠ Y293), M485 (≠ R480), V486 (≠ S481)
binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (≠ D390), G401 (≠ S391), Q402 (≠ A392), H403 (≠ Q393), G426 (= G418), A427 (= A419), M428 (≠ L420), G452 (= G444), D453 (= D445), G454 (= G446), S455 (≠ G447), M458 (≠ F450), N480 (= N472), H482 (≠ E477), L483 (≠ I478), G484 (≠ A479), M485 (≠ R480), V486 (≠ S481)

Sites not aligning to the query:

5k6tA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylamino-carbonyl-triazolinone herbicide, propoxycarbazone-sodium (see paper)
29% identity, 85% coverage: 13:483/551 of query aligns to 16:488/582 of 5k6tA

query
sites
5k6tA

E

D

A

I

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L

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V

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E

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A

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A

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A

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A

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A

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G

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G

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G

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D

V
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V

A

K

A

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A

L

L

-

Q

-

G

-

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A

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A

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E

N

W

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A

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-

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-

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x
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x
M

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A

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Q

L

-

Y

F

Y

Y

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R

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x
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|
G

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x
M

G

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G

I

L

P

P

A

A

A

A

I

I

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G

A

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V

A

A

A

N

P

P

E

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A

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V

I

V

C

D

I

I

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G

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D

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G

G
x
S

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x
M

S

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P

Q

E

E

I

L

M

A

T

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A

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E

A

N

L

L

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P

I

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F

V

I

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V

L

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L

N

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|
N

H

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-

Y

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x
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x
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x
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x
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Q

active site: Y33 (≠ I30), G35 (= G32), G36 (≠ V33), A37 (≠ H34), S38 (≠ T35), E59 (= E55), T82 (≠ I78), F121 (≠ L119), Q122 (≠ H120), E123 (= E121), K171 (≠ L173), M266 (≠ S264), V293 (≠ A294), V400 (≠ D390), G426 (= G418), M428 (≠ L420), D453 (= D445), N480 (= N472), H482 (≠ E477), L483 (≠ I478), M485 (≠ R480), V486 (≠ S481)
binding methyl 2-[(4-methyl-5-oxidanylidene-3-propoxy-1,2,4-triazol-1-yl)carbonylsulfamoyl]benzoate: H267 (≠ L265), D291 (≠ M292), R292 (≠ Y293), M485 (≠ R480)
binding flavin-adenine dinucleotide: R161 (= R163), G222 (= G221), G223 (= G222), G224 (= G223), T246 (= T245), L247 (≠ V246), M248 (≠ N247), M263 (≠ A261), L264 (≠ S262), G265 (≠ P263), M266 (≠ S264), H267 (≠ L265), G286 (= G282), V287 (≠ T283), R288 (≠ E284), D290 (= D291), R292 (≠ Y293), V293 (≠ A294), I309 (= I307), D310 (= D308), I311 (≠ L309), D312 (≠ C310), E315 (≠ Q313), G328 (= G325), D329 (= D326), V330 (= V327), Q404 (≠ P394), M405 (≠ I395), G423 (≠ A414), G424 (= G416)
binding magnesium ion: D453 (= D445), N480 (= N472), H482 (≠ E477)
binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: V400 (≠ D390), G401 (≠ S391), Q402 (≠ A392), H403 (≠ Q393), G426 (= G418), M428 (≠ L420), G452 (= G444), D453 (= D445), G454 (= G446), S455 (≠ G447), M458 (≠ F450), N480 (= N472), H482 (≠ E477), L483 (≠ I478), G484 (≠ A479), M485 (≠ R480), V486 (≠ S481)

Sites not aligning to the query:

5k6rA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylamino-carbonyl-triazolinone herbicide, thiencarbazone-methyl (see paper)
29% identity, 85% coverage: 13:483/551 of query aligns to 16:488/582 of 5k6rA

query
sites
5k6rA

E

D

A

I

L

L

V

V

E

E

E

A

L

L

A

E

A

R

R

Q

G

G

V

V

Q

E

H

T

V

V

F

F

G

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Y

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P

G
|
G

V
x
G

H
x
A

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x
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V

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E

E

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Y

H

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A

L

L

G

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R

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S

D

S

-

S

L

I

R

R

H

N

I

V

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L

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P

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R

H

H

E
|
E

Q

Q

G

G

A

G

G

V

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F

M

A

A

A

D

E

G

G

Y

Y

A

A

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S

S

G

G

R

K

P

P

G

G

V

I

A

C

F

I

V

A

I
x
T

T

S

G

G

P

P

G

G

L

A

T

T

N

N

T

L

L

V

T

S

P

G

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A

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D

A

A

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A

L

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D

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S

V

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M

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x
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x
M

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G

G

I

L

P

P

A

A

A

A

I

I

G

G

A

A

A

S

V

V

A

A

A

N

P

P

E

D

T

A

P

I

V

V

I

V

C

D

I

I

T

D

G
|
G

D
|
D

G
|
G

G
x
S

A

F

Q

I

F
x
M

S

N

L

V

P

Q

E

E

I

L

M

A

T

T

A

I

V

R

D

V

E

E

A

N

L

L

S

P

I

V

T

K

F

V

I

L

V

L

W

L

N

N

N
|
N

H

-

G

-

Y

-

Q

Q

E
x
H

I
x
L

A
x
G

R
x
M

S
x
V

M

M

Q

Q

active site: Y33 (≠ I30), G35 (= G32), G36 (≠ V33), A37 (≠ H34), S38 (≠ T35), E59 (= E55), T82 (≠ I78), F121 (≠ L119), Q122 (≠ H120), E123 (= E121), K171 (≠ L173), M266 (≠ S264), V293 (≠ A294), V400 (≠ D390), G426 (= G418), M428 (≠ L420), D453 (= D445), N480 (= N472), H482 (≠ E477), L483 (≠ I478), M485 (≠ R480), V486 (≠ S481)
binding methyl 4-[(3-methoxy-4-methyl-5-oxidanylidene-1,2,4-triazol-1-yl)carbonylsulfamoyl]-5-methyl-thiophene-3-carboxylate: D291 (≠ M292), R292 (≠ Y293)
binding flavin-adenine dinucleotide: R161 (= R163), G222 (= G221), G223 (= G222), G224 (= G223), T246 (= T245), L247 (≠ V246), M248 (≠ N247), M263 (≠ A261), L264 (≠ S262), G265 (≠ P263), M266 (≠ S264), H267 (≠ L265), G286 (= G282), V287 (≠ T283), R288 (≠ E284), D290 (= D291), R292 (≠ Y293), V293 (≠ A294), I309 (= I307), D310 (= D308), I311 (≠ L309), D312 (≠ C310), E315 (≠ Q313), G328 (= G325), D329 (= D326), V330 (= V327), Q404 (≠ P394), M405 (≠ I395), G423 (≠ A414), G424 (= G416)
binding magnesium ion: D453 (= D445), N480 (= N472), H482 (≠ E477)
binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: V400 (≠ D390), G401 (≠ S391), Q402 (≠ A392), H403 (≠ Q393), G426 (= G418), M428 (≠ L420), G452 (= G444), D453 (= D445), G454 (= G446), S455 (≠ G447), M458 (≠ F450), N480 (= N472), H482 (≠ E477), L483 (≠ I478), G484 (≠ A479), M485 (≠ R480), V486 (≠ S481)

Sites not aligning to the query:

1z8nA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with an imidazolinone herbicide, imazaquin (see paper)
29% identity, 85% coverage: 13:483/551 of query aligns to 16:488/582 of 1z8nA

query
sites
1z8nA

E

D

A

I

L

L

V

V

E

E

E

A

L

L

A

E

A

R

R

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G

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V

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E

H

T

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F

G

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P

G
|
G

V
x
G

H
x
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E

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A

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L

G

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S

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R

R

H

N

I

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P

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R

H

H

E
|
E

Q

Q

G

G

A

G

G

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F

F

M

A

A

A

D

E

G

G

Y

Y

A

A

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R

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S

S

G

G

R

K

P

P

G

G

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I

A

C

F

I

V

A

I
x
T

T

S

G

G

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P

G

G

L

A

T

T

N

N

T

L

L

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P

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A

A

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A

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A

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D

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E

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A

-

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A

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K

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A

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A

F

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I

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A

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A

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x
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x
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x
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V

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A

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A

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active site: Y33 (≠ I30), G35 (= G32), G36 (≠ V33), A37 (≠ H34), S38 (≠ T35), E59 (= E55), T82 (≠ I78), F121 (≠ L119), Q122 (≠ H120), E123 (= E121), K171 (≠ L173), M266 (≠ S264), V293 (≠ A294), V400 (≠ D390), G426 (= G418), M428 (≠ L420), D453 (= D445), N480 (= N472), H482 (≠ E477), L483 (≠ I478), M485 (≠ R480), V486 (≠ S481)
binding 2-(4-isopropyl-4-methyl-5-oxo-4,5-dihydro-1h-imidazol-2-yl)quinoline-3-carboxylic acid: K135 (≠ L134), G160 (≠ S162), R161 (= R163), G190 (= G192), Y191 (≠ E193), R194 (= R196), M195 (≠ T197), P196 (≠ V198), D291 (≠ M292), R292 (≠ Y293), I311 (≠ L309), D312 (≠ C310)
binding flavin-adenine dinucleotide: D100 (= D96), R161 (= R163), G222 (= G221), G223 (= G222), G224 (= G223), T246 (= T245), L247 (≠ V246), M248 (≠ N247), M263 (≠ A261), L264 (≠ S262), G265 (≠ P263), M266 (≠ S264), H267 (≠ L265), G286 (= G282), V287 (≠ T283), R288 (≠ E284), D290 (= D291), R292 (≠ Y293), V293 (≠ A294), D310 (= D308), I311 (≠ L309), E315 (≠ Q313), G328 (= G325), D329 (= D326), V330 (= V327), Q404 (≠ P394), M405 (≠ I395), G423 (≠ A414), G424 (= G416)
binding magnesium ion: D453 (= D445), N480 (= N472)
binding thiamine diphosphate: V400 (≠ D390), G401 (≠ S391), Q402 (≠ A392), H403 (≠ Q393), G426 (= G418), M428 (≠ L420), G452 (= G444), D453 (= D445), G454 (= G446), S455 (≠ G447), M458 (≠ F450), N480 (= N472), H482 (≠ E477), L483 (≠ I478), G484 (≠ A479), M485 (≠ R480), V486 (≠ S481)

Sites not aligning to the query:

active site: 489, 558
binding 2-(4-isopropyl-4-methyl-5-oxo-4,5-dihydro-1h-imidazol-2-yl)quinoline-3-carboxylic acid: 489, 568, 569

1yi1A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, tribenuron methyl (see paper)
29% identity, 85% coverage: 13:483/551 of query aligns to 16:488/582 of 1yi1A

query
sites
1yi1A

E

D

A

I

L

L

V

V

E

E

E

A

L

L

A

E

A

R

R

Q

G

G

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E

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G

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x
G

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x
A

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x
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E

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I

Y

H

R

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G

A

L

L

G

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S

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I

R

R

H

N

I

V

T

L

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P

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R

H

H

E
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E

Q

Q

G

G

A

G

G

V

F

F

M

A

A

A

D

E

G

G

Y

Y

A

A

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S

G

G

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K

P

P

G

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I

A

C

F

I

V

A

I
x
T

T

S

G

G

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P

G

G

L

A

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N

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T

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Y