SitesBLAST
Comparing GFF3909 FitnessBrowser__Marino:GFF3909 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
5x1yB Structure of mercuric reductase from lysinibacillus sphaericus (see paper)
35% identity, 62% coverage: 237:691/729 of query aligns to 3:450/454 of 5x1yB
- active site: A13 (≠ S247), V37 (≠ M271), C41 (= C275), C46 (= C280), S49 (= S283), A74 (≠ P308), G75 (≠ V309), Y178 (≠ P417), E182 (= E421), A318 (= A557), A437 (≠ H678), Y439 (= Y680), E444 (= E685)
- binding flavin-adenine dinucleotide: I9 (= I243), G12 (= G246), I32 (= I266), E33 (= E267), R34 (≠ K268), G39 (= G273), T40 (≠ D274), C41 (= C275), G45 (= G279), C46 (= C280), K50 (= K284), A114 (= A348), T138 (= T377), G139 (= G378), Y178 (≠ P417), R266 (= R505), G305 (= G544), D306 (= D545), F313 (= F552), V314 (≠ T553), A317 (= A556)
D9J041 Mercuric reductase; Hg(II) reductase; EC 1.16.1.1 from Lysinibacillus sphaericus (Bacillus sphaericus) (see paper)
35% identity, 62% coverage: 237:691/729 of query aligns to 84:531/546 of D9J041
- C122 (= C275) modified: Disulfide link with 127, Redox-active
- C127 (= C280) modified: Disulfide link with 122, Redox-active
P16171 Mercuric reductase; Hg(II) reductase; EC 1.16.1.1 from Bacillus cereus (see paper)
34% identity, 63% coverage: 235:690/729 of query aligns to 167:615/631 of P16171
- Y264 (≠ S332) mutation to F: 30-fold decrease in activity. 300-fold decrease in activity; when associated with F-605.
- Y605 (= Y680) mutation to F: 10-fold decrease in activity. 300-fold decrease in activity; when associated with F-264.; mutation to H: 2-fold decrease in activity.
2yquB Crystal structures and evolutionary relationship of two different lipoamide dehydrogenase(e3s) from thermus thermophilus
35% identity, 63% coverage: 238:696/729 of query aligns to 2:451/455 of 2yquB
- active site: P11 (≠ S247), L36 (≠ M271), C40 (= C275), C45 (= C280), S48 (= S283), G72 (= G311), V73 (≠ S312), V177 (≠ P417), E181 (= E421), S314 (≠ A557), H432 (= H678), H434 (≠ Y680), E439 (= E685)
- binding carbonate ion: A310 (≠ T553), S314 (≠ A557), S423 (≠ G669), D426 (≠ K672)
- binding flavin-adenine dinucleotide: G8 (= G244), G10 (= G246), P11 (≠ S247), G12 (≠ A248), E31 (= E267), K32 (= K268), G38 (= G273), T39 (≠ D274), C40 (= C275), R42 (≠ N277), G44 (= G279), C45 (= C280), K49 (= K284), T110 (≠ E347), A111 (= A348), T137 (= T377), G138 (= G378), I178 (= I418), Y265 (≠ N508), G301 (= G544), D302 (= D545), M308 (≠ Q551), L309 (≠ F552), A310 (≠ T553), H311 (= H554)
2yquA Crystal structures and evolutionary relationship of two different lipoamide dehydrogenase(e3s) from thermus thermophilus
35% identity, 63% coverage: 238:696/729 of query aligns to 2:451/455 of 2yquA
- active site: P11 (≠ S247), L36 (≠ M271), C40 (= C275), C45 (= C280), S48 (= S283), G72 (= G311), V73 (≠ S312), V177 (≠ P417), E181 (= E421), S314 (≠ A557), H432 (= H678), H434 (≠ Y680), E439 (= E685)
- binding flavin-adenine dinucleotide: G8 (= G244), G10 (= G246), P11 (≠ S247), G12 (≠ A248), E31 (= E267), K32 (= K268), G38 (= G273), T39 (≠ D274), C40 (= C275), R42 (≠ N277), G44 (= G279), C45 (= C280), K49 (= K284), T110 (≠ E347), A111 (= A348), T137 (= T377), G138 (= G378), S157 (= S397), I178 (= I418), Y265 (≠ N508), G301 (= G544), D302 (= D545), M308 (≠ Q551), L309 (≠ F552), A310 (≠ T553)
2eq7A Crystal structure of lipoamide dehydrogenase from thermus thermophilus hb8 with psbdo
35% identity, 63% coverage: 238:696/729 of query aligns to 2:451/452 of 2eq7A
- active site: P11 (≠ S247), L36 (≠ M271), C40 (= C275), C45 (= C280), S48 (= S283), G72 (= G311), V73 (≠ S312), V177 (≠ P417), E181 (= E421), S314 (≠ A557), H432 (= H678), H434 (≠ Y680), E439 (= E685)
- binding flavin-adenine dinucleotide: G10 (= G246), P11 (≠ S247), G12 (≠ A248), E31 (= E267), K32 (= K268), G38 (= G273), T39 (≠ D274), C40 (= C275), R42 (≠ N277), G44 (= G279), C45 (= C280), K49 (= K284), T110 (≠ E347), A111 (= A348), T137 (= T377), G138 (= G378), S157 (= S397), I178 (= I418), R262 (= R505), Y265 (≠ N508), D302 (= D545), M308 (≠ Q551), L309 (≠ F552), A310 (≠ T553), H311 (= H554), Y341 (≠ F587)
- binding nicotinamide-adenine-dinucleotide: W146 (≠ I386), G174 (= G414), G176 (= G416), V177 (≠ P417), I178 (= I418), E197 (= E437), Y198 (≠ M438), V231 (≠ A471), V260 (= V503), G261 (= G504), R262 (= R505), M308 (≠ Q551), L309 (≠ F552), V339 (= V585)
4k8dA Crystal structure of the c558(464)a/c559(465)a double mutant of tn501 mera in complex with NADPH and hg2+
36% identity, 62% coverage: 242:696/729 of query aligns to 8:456/466 of 4k8dA
- active site: G13 (≠ S247), I37 (≠ M271), C41 (= C275), C46 (= C280), S49 (= S283), V75 (= V307), P76 (= P308), V185 (≠ P417), E189 (= E421), A320 (= A557), F438 (≠ H678), Y440 (= Y680), E445 (= E685)
- binding flavin-adenine dinucleotide: I9 (= I243), G10 (= G244), G12 (= G246), A14 (= A248), E33 (= E267), R34 (≠ K268), G39 (= G273), T40 (≠ D274), C41 (= C275), G45 (= G279), C46 (= C280), K50 (= K284), E115 (= E347), A116 (= A348), T145 (= T377), G146 (= G378), R268 (= R505), G307 (= G544), D308 (= D545), F315 (= F552), V316 (≠ T553), Y317 (≠ H554)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: S183 (≠ G415), S184 (≠ G416), V185 (≠ P417), V186 (≠ I418), E189 (= E421), R206 (≠ G439), N207 (≠ E440), R212 (≠ K445), T266 (≠ V503), G267 (= G504), Q314 (= Q551), F315 (= F552), V345 (= V585)
Sites not aligning to the query:
P00392 Mercuric reductase; Hg(II) reductase; EC 1.16.1.1 from Pseudomonas aeruginosa (see 2 papers)
35% identity, 66% coverage: 217:696/729 of query aligns to 82:551/561 of P00392
- A110 (≠ G249) binding
- G130 (≠ H269) binding
- T135 (≠ D274) binding
- C136 (= C275) modified: Disulfide link with 141, Redox-active
- C141 (= C280) modified: Disulfide link with 136, Redox-active
- K145 (= K284) binding
- A211 (= A348) binding
- D403 (= D545) binding
- V411 (≠ T553) binding
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 558 binding
- 559 binding
6uziC Crystal structure of dihydrolipoyl dehydrogenase from elizabethkingia anophelis nuhp1
32% identity, 62% coverage: 238:691/729 of query aligns to 7:459/470 of 6uziC
- active site: C45 (= C275), C50 (= C280), S53 (= S283), V187 (≠ P417), E191 (= E421), H448 (≠ Y680), E453 (= E685)
- binding flavin-adenine dinucleotide: I12 (= I243), G13 (= G244), G15 (= G246), P16 (≠ S247), G17 (≠ A248), E36 (= E267), K37 (= K268), G43 (= G273), T44 (≠ D274), C45 (= C275), G49 (= G279), C50 (= C280), S53 (= S283), K54 (= K284), V117 (≠ E347), G118 (≠ A348), T147 (= T377), G148 (= G378), I188 (= I418), R276 (= R505), D316 (= D545), M322 (≠ Q551), L323 (≠ F552), A324 (≠ T553)
- binding zinc ion: H448 (≠ Y680), E453 (= E685)
P11959 Dihydrolipoyl dehydrogenase; Dihydrolipoamide dehydrogenase; E3 component of pyruvate complex; EC 1.8.1.4 from Geobacillus stearothermophilus (Bacillus stearothermophilus) (see paper)
33% identity, 63% coverage: 236:691/729 of query aligns to 8:457/470 of P11959
- 39:47 (vs. 267:275, 56% identical) binding
- K56 (= K284) binding
- D314 (= D545) binding
- A322 (≠ T553) binding
1ebdA Dihydrolipoamide dehydrogenase complexed with the binding domain of the dihydrolipoamide acetylase (see paper)
33% identity, 63% coverage: 236:691/729 of query aligns to 2:451/455 of 1ebdA
- active site: P13 (≠ S247), L37 (≠ M271), C41 (= C275), C46 (= C280), S49 (= S283), N74 (≠ P308), V75 (= V309), Y180 (≠ P417), E184 (= E421), S320 (≠ A557), H438 (= H678), H440 (≠ Y680), E445 (= E685)
- binding flavin-adenine dinucleotide: G10 (= G244), G12 (= G246), P13 (≠ S247), V32 (≠ I266), E33 (= E267), K34 (= K268), G39 (= G273), V40 (≠ D274), C41 (= C275), G45 (= G279), C46 (= C280), K50 (= K284), E112 (= E347), A113 (= A348), T141 (= T377), G142 (= G378), Y180 (≠ P417), I181 (= I418), R268 (= R505), D308 (= D545), A314 (≠ Q551), L315 (≠ F552), A316 (≠ T553)
2r9zB Glutathione amide reductase from chromatium gracile (see paper)
34% identity, 62% coverage: 234:685/729 of query aligns to 2:440/453 of 2r9zB
- active site: S13 (= S247), L37 (≠ M271), C41 (= C275), C46 (= C280), K49 (≠ S283), G74 (≠ P308), Y174 (≠ P417), E178 (= E421), I312 (≠ A557), A433 (≠ H678), H435 (≠ Y680), E440 (= E685)
- binding flavin-adenine dinucleotide: G12 (= G246), S13 (= S247), G14 (≠ A248), I32 (= I266), E33 (= E267), S34 (≠ K268), G39 (= G273), T40 (≠ D274), C41 (= C275), G45 (= G279), C46 (= C280), K49 (≠ S283), H111 (≠ E347), A112 (= A348), T136 (= T377), G137 (= G378), I175 (= I418), R260 (= R505), G299 (= G544), D300 (= D545), Q306 (= Q551), L307 (≠ F552), T308 (= T553)
4k7zA Crystal structure of the c136(42)a/c141(47)a double mutant of tn501 mera in complex with NADP and hg2+
35% identity, 62% coverage: 242:696/729 of query aligns to 9:457/467 of 4k7zA
- active site: G14 (≠ S247), I38 (≠ M271), A42 (≠ C275), A47 (≠ C280), S50 (= S283), V76 (= V307), P77 (= P308), V186 (≠ P417), E190 (= E421), A321 (= A557), F439 (≠ H678), Y441 (= Y680), E446 (= E685)
- binding flavin-adenine dinucleotide: I10 (= I243), G11 (= G244), G13 (= G246), A15 (= A248), E34 (= E267), R35 (≠ K268), G40 (= G273), T41 (≠ D274), A42 (≠ C275), G46 (= G279), A47 (≠ C280), K51 (= K284), E116 (= E347), A117 (= A348), T146 (= T377), G147 (= G378), R269 (= R505), G308 (= G544), D309 (= D545), Q315 (= Q551), F316 (= F552), V317 (≠ T553), Y318 (≠ H554)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: S184 (≠ G415), S185 (≠ G416), V186 (≠ P417), V187 (≠ I418), E190 (= E421), R207 (≠ G439), N208 (≠ E440), R213 (≠ K445), T267 (≠ V503), G268 (= G504), R269 (= R505), Q315 (= Q551), F316 (= F552), V346 (= V585)
Sites not aligning to the query:
2rabA Structure of glutathione amide reductase from chromatium gracile in complex with NAD (see paper)
34% identity, 62% coverage: 234:685/729 of query aligns to 2:438/451 of 2rabA
- active site: S13 (= S247), L37 (≠ M271), C41 (= C275), C46 (= C280), K49 (≠ S283), Y173 (≠ P417), E177 (= E421), I310 (≠ A557), A431 (≠ H678), H433 (≠ Y680), E438 (= E685)
- binding flavin-adenine dinucleotide: G10 (= G244), G12 (= G246), S13 (= S247), G14 (≠ A248), I32 (= I266), E33 (= E267), S34 (≠ K268), T40 (≠ D274), G45 (= G279), C46 (= C280), K49 (≠ S283), H110 (≠ E347), A111 (= A348), T135 (= T377), G136 (= G378), R258 (= R505), G297 (= G544), D298 (= D545), Q304 (= Q551), L305 (≠ F552), T306 (= T553)
- binding nicotinamide-adenine-dinucleotide: K49 (≠ S283), I169 (≠ L413), G172 (= G416), Y173 (≠ P417), I174 (= I418), E177 (= E421), A193 (≠ E437), L194 (≠ M438), E195 (≠ G439), V227 (≠ A471), V256 (= V503), G257 (= G504), Q304 (= Q551), V337 (= V585)
D0VWY5 Glutathione amide reductase; GAR; EC 1.8.1.16 from Marichromatium gracile (Chromatium gracile) (see 2 papers)
33% identity, 62% coverage: 234:685/729 of query aligns to 3:442/463 of D0VWY5
- Q3 (≠ E234) binding
- H4 (= H235) binding
- SG 14:15 (≠ SA 247:248) binding
- E34 (= E267) binding
- T41 (≠ D274) binding
- C42 (= C275) modified: Disulfide link with 47, Redox-active
- C47 (= C280) modified: Disulfide link with 42, Redox-active
- K50 (≠ S283) binding ; binding
- HA 113:114 (≠ EA 347:348) binding
- 174:180 (vs. 415:421, 57% identical) binding
- LE 197:198 (≠ MG 438:439) binding
- V230 (≠ A471) binding
- G261 (= G504) binding
- D302 (= D545) binding
- Q308 (= Q551) binding
- QLT 308:310 (≠ QFT 551:553) binding
- V341 (= V585) binding
- H437 (≠ Y680) active site, Proton acceptor; binding
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 binding
- 2:463 modified: mature protein, Glutathione amide reductase
3urhB Crystal structure of a dihydrolipoamide dehydrogenase from sinorhizobium meliloti 1021
33% identity, 62% coverage: 238:691/729 of query aligns to 1:456/465 of 3urhB
- active site: Y35 (vs. gap), C39 (= C275), C44 (= C280), S47 (= S283), V183 (≠ P417), E187 (= E421), H443 (= H678), H445 (≠ Y680), E450 (= E685)
- binding flavin-adenine dinucleotide: I6 (= I243), G7 (= G244), G9 (= G246), P10 (≠ S247), G11 (≠ A248), E30 (= E267), K31 (= K268), G37 (= G273), T38 (≠ D274), C39 (= C275), G43 (= G279), C44 (= C280), K48 (= K284), T111 (≠ E347), G112 (≠ A348), A140 (= A376), T141 (= T377), G142 (= G378), I184 (= I418), R273 (= R505), G312 (= G544), D313 (= D545), M319 (≠ Q551), L320 (≠ F552), A321 (≠ T553), H322 (= H554)
6awaA 1.83 angstrom resolution crystal structure of dihydrolipoyl dehydrogenase from pseudomonas putida in complex with fad and adenosine-5'-monophosphate.
32% identity, 62% coverage: 238:691/729 of query aligns to 5:462/475 of 6awaA
- active site: L45 (≠ M271), C49 (= C275), C54 (= C280), S57 (= S283), V191 (≠ P417), E195 (= E421), F449 (≠ H678), H451 (≠ Y680), E456 (= E685)
- binding adenosine monophosphate: I187 (≠ L413), E211 (= E437), A212 (≠ M438), L213 (≠ G439), V245 (≠ A471), V277 (= V503)
- binding flavin-adenine dinucleotide: I10 (= I243), G13 (= G246), P14 (≠ S247), G15 (≠ A248), E34 (= E267), K35 (= K268), T48 (≠ D274), C49 (= C275), G53 (= G279), C54 (= C280), K58 (= K284), H121 (≠ E347), G122 (≠ A348), S151 (≠ T377), G152 (= G378), I192 (= I418), R279 (= R505), G318 (= G544), D319 (= D545), M325 (≠ Q551), L326 (≠ F552), A327 (≠ T553), Y358 (≠ F587)
Sites not aligning to the query:
2qaeA Crystal structure analysis of trypanosoma cruzi lipoamide dehydrogenase
34% identity, 60% coverage: 256:691/729 of query aligns to 21:455/465 of 2qaeA
- active site: L37 (≠ M271), C41 (= C275), C46 (= C280), S49 (= S283), V184 (≠ P417), E188 (= E421), H442 (= H678), H444 (≠ Y680), E449 (= E685)
- binding flavin-adenine dinucleotide: E32 (= E267), K33 (= K268), R34 (≠ H269), G39 (= G273), T40 (≠ D274), C41 (= C275), G45 (= G279), C46 (= C280), K50 (= K284), E114 (= E347), G115 (≠ A348), T144 (= T377), G145 (= G378), S164 (= S397), I185 (= I418), F274 (≠ N508), G310 (= G544), D311 (= D545), M318 (≠ F552), L319 (≠ T553), A320 (≠ H554), H321 (≠ A555)
Sites not aligning to the query:
4jdrA Dihydrolipoamide dehydrogenase of pyruvate dehydrogenase from escherichia coli (see paper)
31% identity, 62% coverage: 239:691/729 of query aligns to 7:455/471 of 4jdrA
- active site: P15 (≠ S247), L40 (≠ M271), C44 (= C275), C49 (= C280), S52 (= S283), E77 (≠ S312), P78 (≠ F313), I184 (≠ P417), E188 (= E421), V324 (≠ A557), H442 (= H678), H444 (≠ Y680), E449 (= E685)
- binding flavin-adenine dinucleotide: G12 (= G244), G14 (= G246), P15 (≠ S247), A16 (= A248), E35 (= E267), R36 (≠ K268), Y37 (≠ H269), V43 (≠ D274), C44 (= C275), G48 (= G279), C49 (= C280), K53 (= K284), L115 (≠ E347), G116 (≠ A348), A144 (≠ T377), G145 (= G378), I185 (= I418), G311 (= G544), D312 (= D545), M318 (≠ Q551), L319 (≠ F552), A320 (≠ T553), H321 (= H554)
Sites not aligning to the query:
P0A9P0 Dihydrolipoyl dehydrogenase; Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes; Glycine cleavage system L protein; EC 1.8.1.4 from Escherichia coli (strain K12) (see 2 papers)
31% identity, 62% coverage: 239:691/729 of query aligns to 8:456/474 of P0A9P0
- K220 (≠ E452) modified: N6-acetyllysine
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
Query Sequence
>GFF3909 FitnessBrowser__Marino:GFF3909
MTLKKWILVALIAAVVVGFIASGGSELLTLENLKENQQSLGNWIDQNLLVAVLGFVVVYV
VVTALSLPGATIMTLAGGAFFGNLYGLAAVSVASTIGASLAFLVARFLMRDTLRKRYGET
VAKMDRGIEKDGAFYLATLRLVPVFPFFLINLAMGLTAMKLRTYALVSWIAMLPGTFVYV
NAGTQLGQIQSTGDIVSADLLLSFALLGLFPLIAKFVVGFIRRRKVYAGWQKPEHFDYNL
LVIGGGSAGLVSAYIAAAVKAKVALIEKHKMGGDCLNTGCVPSKALIRSAKAADTLRHAN
RYGLESVPVKGSFKNIMNRVKNVIAKVEPHDSPERYRKLGVDCIAGEASFVSPWELEVRH
NDGRTERLTARSIVVATGGKPAVPPIPGLKDMEPLTSDNLWELQEQPERLLVLGGGPIGS
ELAHAFARLGSKVTQVEMGERLLAKEDEDVSELVLKQFQADGVDVRLKHAAAEFRMEEGE
KVAYCEHEGERVRIPFDQVLVAVGRAANTAGLNLERIGVDTLPNGTVPVEEDMSLRYPNV
FACGDVAGPYQFTHAAAHQAWYAAVNGLFGQFKRFKVDYRVMPWVTFTSPEVARVGLSEA
EATAQGVAYEVTRYGLDDLDRAIAESEDHGFIKVLTPPGKDKILGAVVVGSHAGEILAEF
TLAMKHGLGLNKILGTIHPYPTWNESAKYAAGEWKRAHAPEGIMKLLEKLHGWRRGKNTN
TPRSIYAPD
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory