SitesBLAST

4husA Crystal structure of streptogramin group a antibiotic acetyltransferase vata from staphylococcus aureus in complex with virginiamycin m1 (see paper)
38% identity, 47% coverage: 70:202/284 of query aligns to 63:188/212 of 4husA

query
sites
4husA

G

G

A

R

F

F

C

C

S

S

I

I

A

G

A

P

G

G

L

T

R

T

V

F

L

I

-

M

-

N

G

G

D

A

R

N

H

H

P

R

I

M

R

D

R

G

V

-

S

S

S

T

H

Y

P

P

F

F

S

H

Y

-

G

-

P

-

Y

-

G

-

K

-

L

L

A

F

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R

D

-

L

M

G

G

A

W

E

E

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Y

Y

E

M

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P

H

S

A

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N

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K

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K

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I

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G

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N

D

D

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W

I

I

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G

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V

Q

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G

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T

K

I

I

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D

G

G

A

A

V

I

A

A

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E

A

A

I

V

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T

T

K

K

D

N

V

V

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A

P

P

Y

Y

A

S

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I

V

V

G

G

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P

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A

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F

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K

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Y

K

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F

F

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E

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A

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W

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N

Y

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D

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L

I

Sites not aligning to the query:

binding virginiamycin m1: 17, 36, 38

4hurA Crystal structure of streptogramin group a antibiotic acetyltransferase vata from staphylococcus aureus in complex with acetyl coenzyme a (see paper)
38% identity, 47% coverage: 70:202/284 of query aligns to 63:188/211 of 4hurA

query
sites
4hurA

G

G

A

R

F

F

C

C

S
|
S

I
|
I

A
x
G

A

P

G

G

L

T

R

T

V

F

L

I

-

M

-

N

G

G

D
x
A

R
x
N

H

H

P

R

I

M

R

D

R

G

V

-

S

S

S

T

H

Y

P

P

F

F

S

H

Y

-

G

-

P

-

Y

-

G

-

K

-

L

L

A

F

R

R

D

-

L

M

G

G

A

W

E

E

T

K

Y

Y

E

M

P

P

H

S

A

L

R

K

F

D

N

L

T

P

K

L

T
x
K

P

G

P

D

V

I

R

E

I

I

G

G

N

N

D

D

V

V

W
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W

I
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I

G
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G

R
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R

G

D

V

V

Q

T

M

I

A
x
M

G

P

G

G

I

V

T

K

I

I

G

G

N

D

G

G

A

A

V

I

A
|
A

G

E

A

A

I

V

V

V

T
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T

K

K

D

N

V

V

P

A

P

P

Y

Y

A

S

V

I

V

V

G
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G

G

G

V

N

P
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P

A

L

R

K

V

F

L
x
I

K
x
R

Y

K

R

R

F

F

A

S

A

D

P

G

L

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I

I

A

E

R

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E

L

A

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L

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L

I

binding acetyl coenzyme *a: S67 (= S74), I68 (= I75), G69 (≠ A76), A79 (≠ D84), N80 (≠ R85), K110 (≠ T124), W120 (= W134), I121 (= I135), G122 (= G136), R123 (= R137), M128 (≠ A142), A140 (= A154), A141 (= A155), T146 (= T160), G156 (= G170), P159 (= P173), I163 (≠ L177), R164 (≠ K178)

Sites not aligning to the query:

binding acetyl coenzyme *a: 51

6x3jA Crystal structure of streptogramin a acetyltransferase vata from staphylococcus aureus in complex with streptogramin analog f0224 (46) (see paper)
38% identity, 47% coverage: 70:202/284 of query aligns to 63:188/206 of 6x3jA

query
sites
6x3jA

G

G

A

R

F

F

C

C

S

S

I

I

A
x
G

A

P

G

G

L

T

R

T

V

F

L

I

-

M

-

N

G

G

D
x
A

R

N

H
|
H

P

R

I

M

R

D

R

G

V

-

S

S

S

T

H

Y

P

P

F

F

S
x
H

Y

-

G

-

P

-

Y

-

G

-

K

-

L
|
L

A

F

R

R

D

-

L

M

G

G

A

W

E

E

T

K

Y

Y

E
x
M

P
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P

H

S

A

L

R

K

F

D

N
x
L

T

P

K

L

T

K

P

G

P

D

V

I

R

E

I

I

G

G

N

N

D

D

V

V

W
|
W

I

I

G
|
G

R

R

G

D

V

V

Q

T

M

I

A

M

G

P

G

G

I

V

T

K

I

I

G

G

N

D

G

G

A

A

V

I

A

A

G

E

A

A

I

V

V

V

T

T

K

K

D

N

V

V

P

A

P

P

Y

Y

A

S

V

I

V

V

G

G

V

N

P

P

A

L

R

K

V

F

L

I

K

R

Y

K

R

R

F

F

A

S

A

D

P

G

L

V

I

I

A

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E

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A

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A

Q

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W

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I

binding (2R)-2-[(3S,4R,5E,10E,12E,14S,16R,26aR)-16-fluoro-14-hydroxy-4,12-dimethyl-1,7,22-trioxo-4,7,8,9,14,15,16,17,24,25,26,26a-dodecahydro-1H,3H,22H-21,18-(azeno)pyrrolo[2,1-c][1,8,4,19]dioxadiazacyclotetracosin-3-yl]propyl isoquinolin-3-ylcarbamate: H91 (≠ S97), L92 (= L105), M101 (≠ E115), P102 (= P116), L107 (≠ N121)
binding thioacetic acid s-{2-[3-(2-hydroxy-3,3-dimethyl-4-phosphonooxy-butyrylamino)-propionylamino]-ethyl} ester: G69 (≠ A76), A79 (≠ D84), H81 (= H86), W120 (= W134), G122 (= G136)

Sites not aligning to the query:

binding (2R)-2-[(3S,4R,5E,10E,12E,14S,16R,26aR)-16-fluoro-14-hydroxy-4,12-dimethyl-1,7,22-trioxo-4,7,8,9,14,15,16,17,24,25,26,26a-dodecahydro-1H,3H,22H-21,18-(azeno)pyrrolo[2,1-c][1,8,4,19]dioxadiazacyclotetracosin-3-yl]propyl isoquinolin-3-ylcarbamate: 51, 53, 55

6x3cA Crystal structure of streptogramin a acetyltransferase vata from staphylococcus aureus in complex with streptogramin analog f1037 (47) (see paper)
38% identity, 47% coverage: 70:202/284 of query aligns to 63:188/207 of 6x3cA

query
sites
6x3cA

G

G

A

R

F

F

C

C

S

S

I

I

A
x
G

A

P

G

G

L

T

R

T

V

F

L

I

-

M

-

N

G
|
G

D

A

R
x
N

H
|
H

P

R

I

M

R

D

R

G

V

-

S

S

S

T

H

Y

P

P

F

F

S
x
H

Y

-

G

-

P

-

Y

-

G

-

K

-

L
|
L

A

F

R

R

D

-

L

M

G

G

A

W

E

E

T

K

Y

Y

E
x
M

P
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P

H

S

A
x
L

R

K

F

D

N

L

T

P

K

L

T

K

P

G

P

D

V

I

R

E

I

I

G

G

N

N

D

D

V

V

W
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W

I

I

G
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G

R
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R

G

D

V

V

Q

T

M

I

A
x
M

G

P

G

G

I

V

T

K

I

I

G

G

N

D

G

G

A

A

V

I

A
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A

G

E

A

A

I

V

V

V

T

T

K

K

D

N

V

V

P

A

P

P

Y

Y

A

S

V

I

V

V

G

G

V

N

P

P

A

L

R

K

V

F

L

I

K

R

Y

K

R

R

F

F

A

S

A

D

P

G

L

V

I

I

A

E

R

E

L

W

E

L

A

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I

binding (3R,4R,5E,10E,12E,14S,16R,26aR)-16-fluoro-14-hydroxy-12-methyl-3-(propan-2-yl)-4-(prop-2-en-1-yl)-3,4,8,9,14,15,16,17,24,25,26,26a-dodecahydro-1H,7H,22H-21,18-(azeno)pyrrolo[2,1-c][1,8,4,19]dioxadiazacyclotetracosine-1,7,22-trione: G78 (= G83), N80 (≠ R85), H81 (= H86), H91 (≠ S97), L92 (= L105), M101 (≠ E115), P102 (= P116), L104 (≠ A118)
binding thioacetic acid s-{2-[3-(2-hydroxy-3,3-dimethyl-4-phosphonooxy-butyrylamino)-propionylamino]-ethyl} ester: G69 (≠ A76), N80 (≠ R85), H81 (= H86), W120 (= W134), G122 (= G136), R123 (= R137), M128 (≠ A142), A140 (= A154), A141 (= A155)

Sites not aligning to the query:

6x3cE Crystal structure of streptogramin a acetyltransferase vata from staphylococcus aureus in complex with streptogramin analog f1037 (47) (see paper)
38% identity, 47% coverage: 70:202/284 of query aligns to 63:188/203 of 6x3cE

query
sites
6x3cE

G

G

A

R

F

F

C

C

S

S

I

I

A
x
G

A

P

G

G

L

T

R

T

V

F

L

I

-

M

-

N

G
|
G

D

A

R
x
N

H
|
H

P

R

I
x
M

R

D

R

G

V

-

S

S

S

T

H

Y

P

P

F

F

S

-

Y

-

G

-

P

-

Y

-

G

-

K
x
H

L
|
L

A

F

R

R

D

-

L

M

G

G

A

W

E

E

T

K

Y

Y

E
x
M

P
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P

H

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A

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F

D

N

L

T

P

K

L

T

K

P

G

P

D

V

I

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E

I

I

G

G

N

N

D

D

V

V

W
|
W

I

I

G
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G

R

R

G

D

V

V

Q

T

M

I

A

M

G

P

G

G

I

V

T

K

I

I

G

G

N

D

G

G

A

A

V

I

A
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A

G

E

A

A

I

V

V

V

T

T

K

K

D

N

V

V

P

A

P

P

Y

Y

A

S

V

I

V

V

G

G

V
x
N

P
|
P

A

L

R

K

V

F

L

I

K

R

Y

K

R

R

F

F

A

S

A

D

P

G

L

V

I

I

A

E

R

E

L

W

E

L

A

A

T

L

A

Q

W

W

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N

Y

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L

I

binding magnesium ion: N158 (≠ V172), P159 (= P173)
binding (3R,4R,5E,10E,12E,14S,16R,26aR)-16-fluoro-14-hydroxy-12-methyl-3-(propan-2-yl)-4-(prop-2-en-1-yl)-3,4,8,9,14,15,16,17,24,25,26,26a-dodecahydro-1H,7H,22H-21,18-(azeno)pyrrolo[2,1-c][1,8,4,19]dioxadiazacyclotetracosine-1,7,22-trione: G78 (= G83), N80 (≠ R85), H81 (= H86), H91 (≠ K104), L92 (= L105), M101 (≠ E115), P102 (= P116)
binding thioacetic acid s-{2-[3-(2-hydroxy-3,3-dimethyl-4-phosphonooxy-butyrylamino)-propionylamino]-ethyl} ester: G69 (≠ A76), H81 (= H86), M83 (≠ I88), W120 (= W134), G122 (= G136), A140 (= A154), A141 (= A155)

Sites not aligning to the query:

1mrlA Crystal structure of streptogramin a acetyltransferase with dalfopristin (see paper)
36% identity, 47% coverage: 70:202/284 of query aligns to 64:189/204 of 1mrlA

query
sites
1mrlA

G

G

A

K

F

F

C

C

S

S

I

I

A

G

A

P

G

G

L

V

R

T

V

I

L

I

-

M

-

N

G

G

D

A

R
x
N

H
|
H

P

R

I

M

R

D

R

G

V

-

S

S

S

T

H

Y

P

P

F

F

S

N

Y
x
L

G

-

P

-

Y

-

Y

F

G

G

K

N

L

-

A

-

R

-

D

-

L

-

G

G

A

W

E

E

T

K

Y

H

E
x
M

P

P

H

K

A

L

R

D

F

Q

N
x
L

T

P

K

I

T

K

P

G

P

D

V

T

R

I

I

I

G

G

N

N

D

D

V

V

W

W

I

I

G

G

R

K

G

D

V

V

Q

V

M

I

A

M

G

P

G

G

I

V

T

K

I

I

G

G

N

D

G

G

A

A

V

I

V

V

A

A

G

N

A

S

I

V

V

V

T

V

K

K

D

D

V

I

P

A

P

P

Y

Y

A

M

V

L

V

A

G

G

V

N

P

P

A

A

R

N

V

E

L

I

K

K

Y

Q

R

R

F

F

A

D

A

Q

P

D

L

T

I

I

A

N

R

Q

L

L

E

L

A

D

T

I

A

K

W

W

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N

Y

W

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P

L

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E

D

T

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L

I

binding 5-(2-diethylamino-ethanesulfonyl)-21-hydroxy-10-isopropyl-11,19-dimethyl-9,26-dioxa-3,15,28-triaza-tricyclo[23.2.1.00,255]octacosa-1(27),12,17,19,25(28)-pentaene-2,8,14,23-tetraone: N81 (≠ R85), H82 (= H86), L93 (≠ Y98), M102 (≠ E115), L108 (≠ N121)

Sites not aligning to the query:

binding 5-(2-diethylamino-ethanesulfonyl)-21-hydroxy-10-isopropyl-11,19-dimethyl-9,26-dioxa-3,15,28-triaza-tricyclo[23.2.1.00,255]octacosa-1(27),12,17,19,25(28)-pentaene-2,8,14,23-tetraone: 37, 39, 54, 56

1kk4A Crystal structure of vat(d) in complex with acetyl-coa (see paper)
36% identity, 47% coverage: 70:202/284 of query aligns to 64:189/205 of 1kk4A

query
sites
1kk4A

G

G

A

K

F

F

C

C

S

S

I
|
I

A
x
G

A

P

G

G

L

V

R

T

V

I

L

I

-

M

-

N

G

G

D

A

R

N

H

H

P

R

I

M

R

D

R

G

V

-

S

S

S

T

H

Y

P

P

F

F

S

N

Y

L

G

-

P

-

Y

-

Y

F

G

G

K

N

L

-

A

-

R

-

D

-

L

-

G

G

A

W

E

E

T

K

Y

H

E

M

P

P

H

K

A

L

R

D

F

Q

N

L

T

P

K

I

T
x
K

P

G

P

D

V

T

R

I

I

I

G

G

N

N

D

D

V

V

W
|
W

I

I

G
|
G

R
x
K

G

D

V

V

Q

V

M

I

A

M

G

P

G

G

I

V

T

K

I

I

G

G

N

D

G

G

A

A

V

I

V

V

A
|
A

G

N

A

S

I

V

V

V

T
x
V

K
|
K

D

D

V

I

P

A

P

P

Y

Y

A

M

V
x
L

V

A

G
|
G

V

N

P
|
P

A

A

R

N

V

E

L
x
I

K

K

Y

Q

R

R

F

F

A

D

A

Q

P

D

L

T

I

I

A

N

R

Q

L

L

E

L

A

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T

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K

W

W

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N

Y

W

P

P

L

I

E

D

T

I

L

I

binding acetyl coenzyme *a: I69 (= I75), G70 (≠ A76), K111 (≠ T124), W121 (= W134), G123 (= G136), K124 (≠ R137), A141 (= A154), A142 (= A155), V147 (≠ T160), K148 (= K161), L155 (≠ V168), G157 (= G170), G158 (= G171), P160 (= P173), I164 (≠ L177)

1khrA Crystal structure of vat(d) in complex with virginiamycin and coenzyme a (see paper)
36% identity, 47% coverage: 70:202/284 of query aligns to 64:189/206 of 1khrA

query
sites
1khrA

G

G

A

K

F

F

C

C

S

S

I

I

A

G

A

P

G

G

L

V

R

T

V

I

L

I

-

M

-

N

G

G

D
x
A

R
x
N

H
|
H

P

R

I
x
M

R

D

R

G

V

-

S

S

S

T

H

Y

P

P

F

F

S

N

Y
x
L

G

-

P

-

Y

-

Y

F

G

G

K

N

L

-

A

-

R

-

D

-

L

-

G

G

A

W

E

E

T

K

Y

H

E

M

P

P

H

K

A

L

R

D

F

Q

N

L

T

P

K

I

T

K

P

G

P

D

V

T

R

I

I

I

G

G

N

N

D

D

V

V

W
|
W

I

I

G

G

R

K

G

D

V

V

Q

V

M

I

A

M

G

P

G

G

I

V

T

K

I

I

G

G

N

D

G

G

A

A

V

I

V

V

A
|
A

G

N

A

S

I

V

V

V

T
x
V

K

K

D

D

V

I

P

A

P

P

Y

Y

A

M

V
x
L

V

A

G
|
G

G

G

V

N

P
|
P

A

A

R

N

V

E

L
x
I

K

K

Y

Q

R

R

F

F

A

D

A

Q

P

D

L

T

I

I

A

N

R

Q

L

L

E

L

A

D

T

I

A

K

W

W

D

N

Y

W

P

P

L

I

E

D

T

I

L

I

binding coenzyme a: H82 (= H86), M84 (≠ I88), W121 (= W134), A141 (= A154), A142 (= A155), V147 (≠ T160), L155 (≠ V168), G157 (= G170), P160 (= P173), I164 (≠ L177)
binding virginiamycin m1: A80 (≠ D84), N81 (≠ R85), H82 (= H86), L93 (≠ Y98)

Sites not aligning to the query:

binding virginiamycin m1: 37, 39, 54

P50870 Streptogramin A acetyltransferase; Virginiamycin acetyltransferase D; Vat(D); EC 2.3.1.- from Enterococcus faecium (Streptococcus faecium) (see paper)
36% identity, 47% coverage: 70:202/284 of query aligns to 64:189/209 of P50870

query
sites
P50870

G

G

A

K

F

F

C

C

S

S

I

I

A

G

A

P

G

G

L

V

R

T

V

I

L

I

-

M

-

N

G

G

D

A

R

N

H
|
H

P

R

I

M

R

D

R

G

V

-

S

S

S

T

H

Y

P

P

F

F

S

N

Y

L

G

-

P

-

Y

-

Y

F

G

G

K

N

L

-

A

-

R

-

D

-

L

-

G

G

A

W

E

E

T

K

Y

H

E

M

P

P

H

K

A

L

R

D

F

Q

N

L

T

P

K

I

T

K

P

G

P

D

V

T

R

I

I

I

G

G

N

N

D

D

V

V

W

W

I

I

G

G

R

K

G

D

V

V

Q

V

M

I

A

M

G

P

G

G

I

V

T

K

I

I

G

G

N

D

G

G

A

A

V

I

V

V

A

A

G

N

A

S

I

V

V

V

T

V

K

K

D

D

V

I

P

A

P

P

Y

Y

A

M

V

L

V

A

G

G

V

N

P

P

A

A

R

N

V

E

L

I

K

K

Y

Q

R

R

F

F

A

D

A

Q

P

D

L

T

I

I

A

N

R

Q

L

L

E

L

A

D

T

I

A

K

W

W

D

N

Y

W

P

P

L

I

E

D

T

I

L

I

H82 (= H86) mutation to A: 105-fold decrease in activity.

3dhoA Structure of streptogramin acetyltransferase in complex with an inhibitor
36% identity, 47% coverage: 70:202/284 of query aligns to 64:189/203 of 3dhoA

query
sites
3dhoA

G

G

A

K

F

F

C

C

S

S

I

I

A

G

A

P

G

G

L

V

R

T

V

I

L

I

-

M

-

N

G

G

D

A

R

N

H

H

P

R

I
x
M

R

D

R

G

V

-

S

S

S

T

H

Y

P

P

F

F

S

N

Y

L

G

-

P

-

Y

-

Y

F

G

G

K

N

L

-

A

-

R

-

D

-

L

-

G

G

A

W

E

E

T

K

Y

H

E

M

P

P

H

K

A

L

R

D

F

Q

N

L

T

P

K

I

T

K

P

G

P

D

V

T

R

I

I

I

G

G

N

N

D

D

V

V

W

W

I

I

G

G

R

K

G

D

V

V

Q

V

M

I

A

M

G

P

G

G

I

V

T

K

I

I

G

G

N

D

G

G

A

A

V
x
I

V

V

A
|
A

A

A

G

N

A

S

I

V

V

V

T
x
V

K

K

D

D

V

I

P

A

P

P

Y

Y

A

M

V
x
L

V

A

G
|
G

G

G

V

N

P
|
P

A

A

R

N

V

E

L
x
I

K

K

Y

Q

R

R

F

F

A

D

A

Q

P

D

L

T

I

I

A

N

R

Q

L

L

E

L

A

D

T

I

A

K

W

W

D

N

Y

W

P

P

L

I

E

D

T

I

L

I

binding 6-bromo-N'-[(1Z)-(3,5-dichloro-2-hydroxyphenyl)methylidene]-2-methylquinoline-4-carbohydrazide: M84 (≠ I88), I139 (≠ V152), A141 (= A154), V147 (≠ T160), L155 (≠ V168), G157 (= G170), P160 (= P173), I164 (≠ L177)

6u9cA The 2.2 a crystal structure of the type b chloramphenicol acetyltransferase from vibrio cholerae in the complex with acetyl coa
38% identity, 45% coverage: 70:198/284 of query aligns to 59:180/206 of 6u9cA

query
sites
6u9cA

G

G

A

S

F

F

C

C

S

S

I

I

A

G

A

S

G

G

-

A

-

V

L

F

R

M

V

M

L

A

G

G

D

N

R

Q

-

G

H

H

P

R

I

S

R

D

R

W

V

I

S

S

S

T

H

F

P

P

F

F

S

F

Y

Y

G

Q

P

D

Y

-

G

-

K

-

L

-

A

-

R

N

D

D

L

N

G

F

A

A

E

D

T

A

Y

R

E

D

P

G

H

F

A

-

R

-

F

-

N

-

T

T

K

R

T

S

P

G

P

D

V

T

R

I

I

I

G

G

N

H

D

D

V

V

W
|
W

I

I

G

G

R

T

G

E

V

A

Q

M

M

I

A

M

G

P

G

G

I

V

T

K

I

I

G

G

N

H

G

G

A

A

V

I

A
|
A

A

S

G

R

A

S

I

V

V

V

T

T

K

K

D

D

V

V

P

A

P

P

Y

Y

A

E

V

V

G

G

G

S

V

N

P

P

A

A

R

K

V

H

L
x
I

K

K

Y

F

R

R

F

F

A

S

A

D

P

V

L

E

I

I

A

A

R

M

L

L

E

L

A

E

T

M

A

A

W

W

D

N

Y

W

P

P

binding acetyl coenzyme *a: W116 (= W134), A136 (= A154), I159 (≠ L177)

6pubA Crystal structure of the type b chloramphenicol acetyltransferase from vibrio cholerae in the complex with crystal violet
38% identity, 45% coverage: 70:198/284 of query aligns to 62:183/210 of 6pubA

query
sites
6pubA

G

G

A

S

F

F

C

C

S

S

I

I

A

G

A

S

G

G

-

A

-

V

L

F

R

M

V

M

L

A

G

G

D

N

R

Q

-

G

H

H

P

R

I

S

R

D

R

W

V

I

S

S

S

T

H

F

P

P

F

F

S

F

Y

Y

G

Q

P

D

Y

-

G

-

K

-

L

-

A

-

R

N

D

D

L

N

G

F

A

A

E

D

T

A

Y

R

E

D

P

G

H

F

A

-

R

-

F

-

N

-

T

T

K

R

T

S

P

G

P

D

V

T

R

I

I

I

G

G

N

H

D

D

V

V

W

W

I

I

G

G

R

T

G

E

V

A

Q

M

M

I

A

M

G

P

G

G

I

V

T

K

I

I

G

G

N

H

G

G

A

A

V

I

A

A

A

S

G

R

A

S

I

V

V

V

T

T

K

K

D

D

V

V

P

A

P

P

Y

Y

A

E

V

V

G

G

G

S

V

N

P

P

A

A

R

K

V

H

L

I

K

K

Y

F

R

R

F

F

A

S

A

D

P

V

L

E

I

I

A

A

R

M

L

L

E

L

A

E

T

M

A

A

W

W

D

N

Y

W

P

P

Sites not aligning to the query:

binding crystal violet: 4, 7, 9, 10, 11, 36, 37

2xatA Complex of the hexapeptide xenobiotic acetyltransferase with chloramphenicol and desulfo-coenzyme a (see paper)
36% identity, 48% coverage: 70:204/284 of query aligns to 58:186/208 of 2xatA

query
sites
2xatA

G

G

A

S

F

F

C

C

S

S

I

I

A

G

A

S

G

G

L

A

R

A

V

F

L

I

-

M

-

A

G

G

D

N

R

Q

-

G

H

H

P

R

I

A

R

E

R

W

V

A

S

S

S

T

H

F

P

P

F

F

S

H

Y

F

G

-

P

-

Y

M

Y

H

G

E

K

E

L

P

A

A

R

F

D

A

L

G

G

A

A

V

E

N

T

G

Y

Y

E

Q

P

P

H

A

A

G

R

D

F

-

N

-

T

-

K

-

T

-

P

-

V

T

R

L

I

I

G

G

N

H

D

E

V

V

W

W

I

I

G
|
G

R
x
T

G

E

V

A

Q

M

M

F

A

M

G

P

G

G

I

V

T

R

I

V

G

G

N

H

G

G

A

A

V

I

A
x
G

A
x
S

G

R

A

A

I

L

V

V

T

T

K

G

D

D

V

V

P

E

P

P

Y

Y

A

A

V

I

V

V

G
|
G

V

N

P

P

A

A

R

R

V

T

L
x
I

K

R

Y

K

R

R

F

F

A

S

A

D

P

G

L

D

I

I

A

Q

R

N

L

L

E

L

A

E

T

M

A

A

W

W

D

D

Y

W

P

P

L

L

E

A

T

D

L

I

A

E

A

A

binding desulfo-coenzyme a: G118 (= G136), T119 (≠ R137), G136 (≠ A154), S137 (≠ A155), G152 (= G170), G153 (= G171), I159 (≠ L177)

Sites not aligning to the query:

binding chloramphenicol: 6, 7, 8, 28

7uujA Crystal structure of aminoglycoside resistance enzyme apma, complex with gentamicin
39% identity, 31% coverage: 115:202/284 of query aligns to 165:254/272 of 7uujA

query
sites
7uujA

E

D

P

P

H

K

A

H

R

P

F

Y

N

A

T

Y

K

S

T

K

P

E

P

P

V

M

R

T

I

I

G

G

N

S

D

D

V

V

W

Y

I

I

G

G

R

A

G

H

-

A

-

F

V

I

Q

N

M

A

A

S

G

T

G

V

I

T

T

S

I

I

G

G

N

D

G

G

A

A

V

I

A

G

A

S

G

G

A

A

I

V

V

V

T

L

K

E

D

N

V

V

P

P

Y

F

A

A

V

V

G

V

G

G

V

V

P

P

A

A

R

R

V

I

L

K

K

R

Y

Y

R

R

F

F

A

S

A

K

P

E

L

M

I

I

A

E

R

T

L

L

E

L

A

R

T

V

A

K

W

W

D

D

Y

W

P

S

L

I

E

E

T

E

L

I

Sites not aligning to the query:

binding (2r,3r,4r,5r)-2-((1s,2s,3r,4s,6r)-4,6-diamino-3-((2r,3r,6s)-3-amino-6-(aminomethyl)-tetrahydro-2h-pyran-2-yloxy)-2-hydroxycyclohexyloxy)-5-methyl-4-(methylamino)-tetrahydro-2h-pyran-3,5-diol: 55, 85, 102, 135, 144, 145

7jm2A Crystal structure of aminoglycoside resistance enzyme apma, complex with apramycin
39% identity, 31% coverage: 115:202/284 of query aligns to 165:254/272 of 7jm2A

query
sites
7jm2A

E

D

P

P

H

K

A

H

R

P

F
x
Y

N

A

T
x
Y

K

S

T

K

P

E

P

P

V

M

R

T

I

I

G

G

N

S

D

D

V

V

W

Y

I

I

G

G

R

A

G

H

-

A

-

F

V

I

Q

N

M

A

A

S

G

T

G

V

I

T

T

S

I

I

G

G

N

D

G

G

A

A

V

I

A

G

A

S

G

G

A

A

I

V

V

V

T

L

K

E

D

N

V

V

P

P

Y

F

A

A

V

V

G

V

G

G

V

V

P

P

A

A

R

R

V

I

L

K

K

R

Y

Y

R

R

F

F

A

S

A

K

P

E

L

M

I

I

A

E

R

T

L

L

E

L

A

R

T

V

A

K

W

W

D

D

Y

W

P

S

L

I

E

E

T

E

L

I

binding apramycin: Y170 (≠ F120), Y172 (≠ T122)

Sites not aligning to the query:

binding apramycin: 85, 102, 135, 144, 145, 147

7jm1A Crystal structure of aminoglycoside resistance enzyme apma, complex with acetyl-coa
39% identity, 31% coverage: 115:202/284 of query aligns to 165:254/272 of 7jm1A

query
sites
7jm1A

E

D

P

P

H

K

A

H

R

P

F

Y

N

A

T

Y

K

S

T

K

P

E

P

P

V

M

R

T

I

I

G

G

N

S

D

D

V

V

W
x
Y

I

I

G
|
G

R

A

G

H

-

A

-
x
F

V

I

Q

N

M

A

A

S

G

T

G

V

I

T

T

S

I

I

G

G

N

D

G

G

A

A

V

I

A
x
G

A
x
S

G

G

A

A

I

V

V

V

T
x
L

K

E

D

N

V

V

P

P

Y

F

A

A

V

V

G
x
V

G

G

V
|
V

P

P

A

A

R

R

V

I

L
x
K

K

R

Y

Y

R

R

F

F

A

S

A

K

P

E

L

M

I

I

A

E

R

T

L

L

E

L

A

R

T

V

A

K

W

W

D

D

Y

W

P

S

L

I

E

E

T

E

L

I

binding acetyl coenzyme *a: Y184 (≠ W134), G186 (= G136), F190 (vs. gap), G206 (≠ A154), S207 (≠ A155), L212 (≠ T160), V222 (≠ G170), V224 (= V172), K229 (≠ L177)

Sites not aligning to the query:

binding acetyl coenzyme *a: 102, 124, 125, 126, 132, 134

7uukC Crystal structure of aminoglycoside resistance enzyme apma, complex with tobramycin (see paper)
39% identity, 31% coverage: 115:202/284 of query aligns to 167:256/276 of 7uukC

query
sites
7uukC

E

D

P

P

H

K

A

H

R

P

F

Y

N

A

T

Y

K

S

T

K

P

E

P

P

V

M

R

T

I

I

G

G

N

S

D

D

V

V

W

Y

I

I

G

G

R

A

G

H

-

A

-

F

V

I

Q

N

M

A

A

S

G

T

G

V

I

T

T

S

I

I

G

G

N

D

G

G

A

A

V

I

A

G

A

S

G

G

A

A

I

V

V

V

T

L

K

E

D

N

V

V

P

P

Y

F

A

A

V

V

G

V

G

G

V

V

P

P

A

A

R

R

V

I

L

K

K

R

Y

Y

R

R

F

F

A

S

A

K

P

E

L

M

I

I

A

E

R

T

L

L

E

L

A

R

T

V

A

K

W

W

D

D

Y

W

P

S

L

I

E

E

T

E

L

I

Sites not aligning to the query:

binding tobramycin: 83, 87, 104, 105, 115, 137, 146, 147

7uulA Crystal structure of aminoglycoside resistance enzyme apma, complex with kanamycin b and coenzyme a (see paper)
39% identity, 31% coverage: 115:202/284 of query aligns to 166:255/274 of 7uulA

query
sites
7uulA

E

D

P

P

H

K

A

H

R

P

F

Y

N

A

T

Y

K

S

T

K

P

E

P

P

V

M

R

T

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I

G

G

N

S

D

D

V

V

W
x
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I

I

G
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G

R
x
A

G

H

-

A

-
x
F

V

I

Q

N

M

A

A

S

G

T

G

V

I

T

T

S

I

I

G

G

N

D

G

G

A

A

V

I

A

G

A
x
S

G

G

A

A

I

V

V

V

T
x
L

K

E

D

N

V

V

P

P

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F

A

A

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V

G
x
V

G

G

V

V

P

P

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A

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R

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L
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K

K
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R

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Y

R

R

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F

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K

P

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M

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I

A

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L

L

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L

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A

K

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W

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E

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E

L

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binding coenzyme a: Y185 (≠ W134), G187 (= G136), A188 (≠ R137), F191 (vs. gap), S208 (≠ A155), L213 (≠ T160), V223 (≠ G170), K230 (≠ L177), R231 (≠ K178)

Sites not aligning to the query:

7uumA Crystal structure of aminoglycoside resistance enzyme apma, complex with paromomycin and coenzyme a (see paper)
39% identity, 31% coverage: 115:202/284 of query aligns to 167:256/274 of 7uumA

query
sites
7uumA

E

D

P

P

H
x
K

A

H

R

P

F

Y

N

A

T

Y

K

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K

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G

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G

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D

G

G

A

A

V

I

A

G

A

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G

G

A

A

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N

V

V

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P

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F

A

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V

G

G

V

V

P
|
P

A

A

R

R

V

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L

K

K

R

Y

Y

R

R

F

F

A

S

A

K

P

E

L

M

I

I

A

E

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L

L

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L

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R

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V

A

K

W

W

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D

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W

P

S

L

I

E

E

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E

L

I

binding coenzyme a: K169 (≠ H117), F192 (vs. gap), P227 (= P173)

Sites not aligning to the query:

binding coenzyme a: 134, 136
binding paromomycin: 114, 115, 137, 146, 147

Query Sequence

>GFF3918 FitnessBrowser__Phaeo:GFF3918
MRRLLADHHVMPKPWRNERDLTALKRISFPLDLSLSVLTRHGLKAGGLLPLSSIGYMSYS
HSPSRNWSAGAFCSIAAGLRVLGDRHPIRRVSSHPFSYGPYYGKLARDLGAETYEPHARF
NTKTPPVRIGNDVWIGRGVQMAGGITIGNGAVVAAGAIVTKDVPPYAVVGGVPARVLKYR
FAAPLIARLEATAWWDYPLETLAAFKMGHPRRFCKAFEPEEANLAKREERWITAADLQAL
AAPVDVARASLSRAITLPEGRTRGPVSKQIRRISDRLTRIVRSA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory