SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF393 FitnessBrowser__Phaeo:GFF393 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

7fixR1 Photosystem I reaction center subunit PsaK (see paper)
49% identity, 21% coverage: 275:349/357 of query aligns to 11:85/97 of 7fixR1

query
sites
7fixR1

D

D

G

G

A

S

T

E

Q

T

T

T

I

I

E

D

I

V

G

P

K

E

D

D

M

E

T

Y

L

I

L

L

D

D

A

V

A

A

L

E

E

E

N

Q

A

G

M

L

D

D

A

L

P

P

Y
x
F

A
x
S

C
|
C

K
x
R

A

A

G
|
G

V

A

C
|
C

S

S

T

T

C
|
C

R

A

C

G

K

K

V

L

L

L

E

E

G

G

E

E

V

V

E

D

M

Q

I

S

A

D

N

Q

H

S

A

F

L

L

E

D

D

D

Y

D

E

Q

V

I

E

E

K

K

G

G

Y

F

V

V

L
|
L

S

T

C
|
C

Q

V

A

A

Y

Y

P

P

L

R

S

S

D

D

binding [2Ga-2S] cluster: F38 (≠ Y302), S39 (≠ A303), C40 (= C304), R41 (≠ K305), G43 (= G307), C45 (= C309), C48 (= C312), L76 (= L340), C78 (= C342)

6khi1 Supercomplex for cylic electron transport in cyanobacteria (see paper)
49% identity, 21% coverage: 275:349/357 of query aligns to 12:86/98 of 6khi1

query
sites
6khi1

D

D

G

G

A

S

T

E

Q

T

T

T

I

I

E

D

I

V

G

P

K

E

D

D

M

E

T

Y

L

I

L

L

D

D

A

V

A

A

L

E

E

E

N

Q

A

G

M

L

D

D

A

L

P

P

Y

F

A

S

C
|
C

K
x
R

A

A

G
|
G

V

A

C
|
C

S

S

T

T

C
|
C

R

A

C

G

K

K

V

L

L

L

E

E

G

G

E

E

V

V

E

D

M

Q

I

S

A

D

N

Q

H

S

A

F

L

L

E

D

D

D

Y

D

E

Q

V

I

E

E

K

K

G

G

Y

F

V

V

L
|
L

S

T

C
|
C

Q

V

A

A

Y

Y

P

P

L

R

S

S

D

D

binding fe2/s2 (inorganic) cluster: C41 (= C304), R42 (≠ K305), G44 (= G307), C46 (= C309), C49 (= C312), L77 (= L340), C79 (= C342)

1rfkB Crystal structure of 2fe2s ferredoxin from thermophilic cyanobacterium mastigocladus laminosus (see paper)
47% identity, 22% coverage: 275:352/357 of query aligns to 12:89/97 of 1rfkB

query
sites
1rfkB

D

E

G

G

A

L

T

N

Q

K

T

T

I

I

E

E

I

V

G

P

K

D

D

D

M

Q

T

Y

L

I

L

L

D

D

A

A

A

A

L

E

E

E

N

A

A

G

M

I

D

D

A

L

P

P

Y
|
Y

A

S

C
|
C

K
x
R

A

A

G
|
G

V

A

C
|
C

S

S

T

T

C
|
C

R

A

C

G

K

K

V

L

L

I

E

S

G

G

E

T

V

V

E

D

M

Q

I

S

A

D

N

Q

H

S

A

F

L

L

E

D

D

D

Y

D

E

Q

V

I

E

E

K

A

G

G

Y

Y

V

V

L

L

S

T

C
|
C

Q

V

A

A

Y

Y

P

P

L

T

S

S

D

D

K

C

V

V

V

I

binding fe2/s2 (inorganic) cluster: Y39 (= Y302), C41 (= C304), R42 (≠ K305), G44 (= G307), C46 (= C309), C49 (= C312), C79 (= C342)

7s3dX Structure of photosystem i with bound ferredoxin from synechococcus sp. Pcc 7335 acclimated to far-red light (see paper)
47% identity, 22% coverage: 280:357/357 of query aligns to 16:93/97 of 7s3dX

query
sites
7s3dX

T

T

I

I

E

E

I

V

G

A

K

D

D

D

M

E

T

Y

L

I

L

L

D

D

A

A

A

A

L

E

E

E

N

Q

A

G

M

I

D

D

A

L

P

P

Y
|
Y

A
x
S

C
|
C

K
x
R

A

A

G
|
G

V

A

C
|
C

S

S

T

T

C
|
C

R

A

C

G

K

K

V

L

L

T

E

E

G

G

E

T

V

V

E

D

M

Q

I

S

A

D

N

Q

H

S

A

F

L

L

E

D

D

D

Y

D

E

Q

V

I

E

E

K

A

G

G

Y

Y

V

V

L

L

S

T

C
|
C

Q

V

A

A

Y

Y

P

P

L

T

S

S

D

D

K

C

V

T

V

V

V

M

D

T

Y

H

D

Q

Q

E

binding fe2/s2 (inorganic) cluster: Y38 (= Y302), S39 (≠ A303), C40 (= C304), R41 (≠ K305), G43 (= G307), C45 (= C309), C48 (= C312), C78 (= C342)

6kbhA Crystal structure of an intact type iv self-sufficient cytochrome p450 monooxygenase
25% identity, 89% coverage: 7:322/357 of query aligns to 456:732/765 of 6kbhA

query
sites
6kbhA

L

M

V

A

V

V

T

S

D

G

V

L

R

E

K

S

T

I

I

A

R

D

D

D

A

I

V

L

V

L

V

I

T

T

L

L

K

R

P

D

V

T

G

S

G

G

A

R

A

P

E

L

E

P

F

-

D

K

F

W

T

S

Q

A

G

G

Q

S

Y

H

L

I

T

-

F

-

R

-

R

-

D

D

F

V

D

D

G

C

E

G

E

A

L

V

R

S

R
|
R

S
x
Q

Y
|
Y

S
|
S

I

L

C

C

A

G

G

D

-

P

K

H

D

D

E

R

G

T

I

T

L

F

Q

Q

V

V

G
x
A

I

V

-
x
L

-

H

-

D

K

R

R

E

V
x
S

D
x
R

G

G

G
|
G

A

-

F

-

S
|
S

T

R

W

W

A

I

N

H

T

T

E

E

L

L

K

A

A

V

G

G

D

A

T

T

L

L

Q

R

A

V

M

R

A

G

P

P

M

R

G

N

S

H

F

F

F

-

T

-

P

K

L

L

N

D

E

P

A

D

A

A

E

-

K

K

H

R

Y

Y

L

V

G

F

F

V

A

A

G

G

G

G

S
x
I

G

G

I

I

T
|
T

P

P

V

V

L

I

S

A

I

M

L

A

K

D

T

Q

T

V

L

K

A

A

A

A

E

-

P

-

N

G

A

G

S

D

F

Y

T

E

L

I

V

H

Y

Y

A

A

N

G

K

R

G

S

V

R

N

T

T

S

I

M

M

A

F

F

R

-

E

-

E

-

L

-

E

-

D

-

L

L

K

D

N

R

L

L

Y

A

M

R

G

D

R

H

F

G

N

E

L

S

I

V

H

R

V

V

L

Y

E

P

S

G

D

D

-

E

A

G

Q

V

E

R

I

M

D

D

L

L

F

P

T

S

G

L

L

F

V

A

T

D

E

P

E

E

K

D

C

G

A

T

Q

Q

L

V

F

Y

E

S

R

-

W

-

I

-

D

-

I

-

K

-

S

-

V

-

D

-

T

-

A

-

F

-

I

-

C

C

G

G

P

P

E

E

P

R

M

L

M

L

L

S

G

A

I

L

A

S

S

E

A

A

L

-

R

-

T

T

A

A

G

H

L

W

N

P

D

D

S

D

Q

T

I

L

K

H

F

V

E
|
E

L

H

F
|
F

A

S

S

S

A

T

Q

L

P

E

G

E

R

L

A

D

K

P

R

S

K

K

V

-

T

-

A

-

G

-

G

-

T

-

G

-

T

-

G

-

A

-

N

-

Q

E

T

H

K

G

A

F

A

D

I

V

T

V

L

L

D

K

G

D

A

S

T

G

Q

I

T

T

I

V

E

P

I

V

G

A

K

A

D

D

M

Q

T

T

L

V

L

L

D

Q

A

A

A

L

L

R

E

A

N

A

A

N

M

I

D

D

A

A

P

Q

Y

S

A

D

C
|
C

K
x
E

A

E

G
|
G

V
x
I

C
|
C

S
x
G

T

A

C
|
C

R

E

C

V

K

P

V

V

L

L

E

D

G

G

E

E

V

V

E

D

binding fe2/s2 (inorganic) cluster: C714 (= C304), E715 (≠ K305), G717 (= G307), C719 (= C309), G720 (≠ S310), C722 (= C312)
binding flavin mononucleotide: R499 (= R55), Q500 (≠ S56), Y501 (= Y57), S502 (= S58), A516 (≠ G71), L518 (vs. gap), S523 (≠ V75), R524 (≠ D76), G526 (= G78), S527 (= S81), I565 (≠ S122), T568 (= T125), E663 (= E242), F665 (= F244), I718 (≠ V308)

Sites not aligning to the query:

active site: 190, 264, 265, 372, 373, 374
binding fe2/s2 (inorganic) cluster: 752
binding protoporphyrin ix containing fe: 67, 104, 105, 112, 116, 261, 262, 265, 266, 308, 311, 313, 364, 365, 370, 372, 373, 374, 378

5aukA Crystal structure of the ga-substituted ferredoxin (see paper)
45% identity, 25% coverage: 265:352/357 of query aligns to 1:87/96 of 5aukA

query
sites
5aukA

A

A

N

S

Q

Y

T

T

K

V

A

K

A

L

I

I

T

T

L

P

D

D

G

G

A

E

T

S

Q

-

T

S

I

I

E

E

I

C

G

S

K

D

D

D

M

T

T

Y

L

I

L

L

D

D

A

A

A

A

L

E

E

E

N

A

A

G

M

L

D

D

A

L

P

P

Y
|
Y

A
x
S

C
|
C

K
x
R

A

A

G
|
G

V

A

C
|
C

S

S

T

T

C
|
C

R

A

C

G

K

K

V

I

L

T

E

A

G

G

E

S

V

V

E

D

M

Q

I

S

A

D

N

Q

H

S

A

F

L

L

E

D

D

D

Y

D

E

Q

V

I

E

E

K

A

G

G

Y

Y

V

V

L

L

S

T

C
|
C

Q

V

A

A

Y

Y

P

P

L

T

S

S

D

D

K

C

V

T

V

I

binding [2Ga-2S] cluster: Y37 (= Y302), S38 (≠ A303), C39 (= C304), R40 (≠ K305), G42 (= G307), C44 (= C309), C47 (= C312), C77 (= C342)

2kajA Nmr structure of gallium substituted ferredoxin (see paper)
45% identity, 25% coverage: 265:352/357 of query aligns to 1:87/96 of 2kajA

query
sites
2kajA

A

A

N

S

Q

Y

T

T

K

V

A

K

A

L

I

I

T

T

L

P

D

D

G

G

A

E

T

S

Q

-

T

S

I

I

E

E

I

C

G

S

K

D

D

D

M

T

T

Y

L

I

L

L

D

D

A

A

A

A

L

E

E

E

N

A

A

G

M

L

D

D

A

L

P

P

Y

Y

A

S

C
|
C

K

R

A

A

G

G

V

A

C
|
C

S

S

T

T

C
|
C

R

A

C

G

K

K

V

I

L

T

E

A

G

G

E

S

V

V

E

D

M

Q

I

S

A

D

N

Q

H

S

A

F

L

L

E

D

D

D

Y

D

E

Q

V

I

E

E

K

A

G

G

Y

Y

V

V

L

L

S

T

C
|
C

Q

V

A

A

Y

Y

P

P

L

T

S

S

D

D

K

C

V

T

V

I

binding gallium (iii) ion: C39 (= C304), C44 (= C309), C47 (= C312), C77 (= C342)

1doxA 1h and 15n sequential assignment, secondary structure and tertiary fold of [2fe-2s] ferredoxin from synechocystis sp. Pcc 6803 (see paper)
45% identity, 25% coverage: 265:352/357 of query aligns to 1:87/96 of 1doxA

query
sites
1doxA

A

A

N

S

Q

Y

T

T

K

V

A

K

A

L

I

I

T

T

L

P

D

D

G

G

A

E

T

S

Q

-

T

S

I

I

E

E

I

C

G

S

K

D

D

D

M

T

T

Y

L

I

L

L

D

D

A

A

A

A

L

E

E

E

N

A

A

G

M

L

D

D

A

L

P

P

Y

Y

A

S

C
|
C

K

R

A

A

G

G

V

A

C
|
C

S

S

T

T

C
|
C

R

A

C

G

K

K

V

I

L

T

E

A

G

G

E

S

V

V

E

D

M

Q

I

S

A

D

N

Q

H

S

A

F

L

L

E

D

D

D

Y

D

E

Q

V

I

E

E

K

A

G

G

Y

Y

V

V

L

L

S

T

C
|
C

Q

V

A

A

Y

Y

P

P

L

T

S

S

D

D

K

C

V

T

V

I

binding fe2/s2 (inorganic) cluster: C39 (= C304), C44 (= C309), C47 (= C312), C77 (= C342)

P27320 Ferredoxin-1; Ferredoxin I from Synechocystis sp. (strain PCC 6803 / Kazusa) (see 3 papers)
45% identity, 25% coverage: 265:352/357 of query aligns to 2:88/97 of P27320

query
sites
P27320

A

A

N

S

Q

Y

T

T

K

V

A

K

A

L

I

I

T

T

L

P

D

D

G

G

A

E

T

S

Q

-

T

S

I

I

E

E

I

C

G

S

K

D

D

D

M

T

T

Y

L

I

L

L

D

D

A

A

A

A

L

E

E

E

N

A

A

G

M

L

D

D

A

L

P

P

Y

Y

A

S

C
|
C

K

R

A

A

G

G

V

A

C
|
C

S

S

T

T

C
|
C

R

A

C

G

K

K

V

I

L

T

E

A

G

G

E

S

V

V

E

D

M

Q

I

S

A

D

N

Q

H

S

A

F

L

L

E

D

D

D

Y

D

E

Q

V

I

E

E

K

A

G

G

Y

Y

V

V

L

L

S

T

C
|
C

Q

V

A

A

Y

Y

P

P

L

T

S

S

D

D

K

C

V

T

V

I

C40 (= C304) binding
C45 (= C309) binding
C48 (= C312) binding
C78 (= C342) binding

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

3b2gA Leptolyngbya boryana ferredoxin (see paper)
42% identity, 23% coverage: 275:357/357 of query aligns to 12:94/98 of 3b2gA

query
sites
3b2gA

D

E

G

G

A

L

T

N

Q

T

T

T

I

I

E

E

I

V

G

P

K

D

D

D

M

E

T

Y

L

I

L

L

D

D

A

A

A

A

L

E

E

E

N

Q

A

G

M

I

D

D

A

L

P

P

Y

Y

A
x
S

C
|
C

K
x
R

A

A

G
|
G

V

A

C
|
C

S

S

T

T

C
|
C

R

A

C

G

K

K

V

I

L

T

E

A

G

G

E

T

V

V

E

D

M

Q

I

S

A

D

N

Q

H

S

A

F

L

L

E

D

D

D

Y

D

E

Q

V

I

E

Q

K

A

G

G

Y

Y

V

V

L

L

S

T

C
|
C

Q

V

A

A

Y

Y

P

P

L

T

S

S

D

D

K

C

V

T

V

I

V

L

D

T

Y

H

D

Q

Q

E

binding fe2/s2 (inorganic) cluster: S40 (≠ A303), C41 (= C304), R42 (≠ K305), G44 (= G307), C46 (= C309), C49 (= C312), C79 (= C342)

P0A3C7 Ferredoxin-1; Ferredoxin I from Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) (see paper)
45% identity, 22% coverage: 275:352/357 of query aligns to 13:90/99 of P0A3C7

query
sites
P0A3C7

D

E

G

G

A

T

T

K

Q

H

T

E

I

I

E

E

I

V

G

P

K

D

D

D

M

E

T

Y

L

I

L

L

D

D

A

A

A

A

L

E

E

E

N

Q

A

G

M

Y

D

D

A

L

P

P

Y

F

A

S

C

C

K
x
R

A

A

G

G

V

A

C

C

S

S

T
|
T

C

C

R

A

C

G

K

K

V

L

L

V

E

S

G

G

E

T

V

V

E

D

M

Q

I

S

A

D

N

Q

H

S

A
x
F

L

L

E

D

D
|
D

Y
x
D

E

Q

V

I

E

E

K

A

G

G

Y

Y

V

V

L

L

S

T

C

C

Q

V

A

A

Y

Y

P

P

L

T

S

S

D

D

K

V

V

V

V

I

R43 (≠ K305) mutation R->A,E,H: No effect on electron transfer.
T49 (= T311) mutation to A: No effect on electron transfer.
F66 (≠ A328) mutation F->A,I: 1000-fold decrease in electron transfer.
D69 (= D331) mutation to K: Small effect on electron transfer.
D70 (≠ Y332) mutation to K: Small effect on electron transfer.

Sites not aligning to the query:

95 E→K: 20000-fold decrease in electron transfer.

1ewyC Anabaena pcc7119 ferredoxin:ferredoxin-NADP+-reductase complex (see paper)
45% identity, 22% coverage: 275:352/357 of query aligns to 12:89/98 of 1ewyC

query
sites
1ewyC

D

E

G

G

A

T

T

K

Q

H

T

E

I

I

E

E

I

V

G

P

K

D

D

D

M

E

T

Y

L

I

L

L

D

D

A

A

A

A

L

E

E

E

N

Q

A

G

M

Y

D

D

A

L

P

P

Y
x
F

A
x
S

C
|
C

K
x
R

A

A

G
|
G

V
x
A

C
|
C

S

S

T

T

C
|
C

R

A

C

G

K

K

V

L

L

V

E

S

G

G

E

T

V

V

E

D

M

Q

I

S

A

D

N

Q

H
x
S

A

F

L

L

E

D

D

D

Y

D

E

Q

V

I

E

E

K

A

G

G

Y

Y

V

V

L
|
L

S

T

C
|
C

Q

V

A

A

Y

Y

P

P

L

T

S

S

D

D

K

V

V

V

V

I

binding flavin-adenine dinucleotide: A45 (≠ V308), S64 (≠ H327)
binding fe2/s2 (inorganic) cluster: F39 (≠ Y302), S40 (≠ A303), C41 (= C304), R42 (≠ K305), G44 (= G307), A45 (≠ V308), C46 (= C309), C49 (= C312), L77 (= L340), C79 (= C342)

1czpA Anabaena pcc7119 [2fe-2s] ferredoxin in the reduced and oxixized state at 1.17 a (see paper)
45% identity, 22% coverage: 275:352/357 of query aligns to 12:89/98 of 1czpA

query
sites
1czpA

D

E

G

G

A

T

T

K

Q

H

T

E

I

I

E

E

I

V

G

P

K

D

D

D

M

E

T

Y

L

I

L

L

D

D

A

A

A

A

L

E

E

E

N

Q

A

G

M

Y

D

D

A

L

P

P

Y
x
F

A

S

C
|
C

K
x
R

A

A

G
|
G

V

A

C
|
C

S

S

T

T

C
|
C

R

A

C

G

K

K

V

L

L

V

E

S

G

G

E

T

V

V

E

D

M

Q

I

S

A

D

N

Q

H

S

A

F

L

L

E

D

D

D

Y

D

E

Q

V

I

E

E

K

A

G

G

Y

Y

V

V

L

L

S

T

C
|
C

Q

V

A

A

Y

Y

P

P

L

T

S

S

D

D

K

V

V

V

V

I

binding fe2/s2 (inorganic) cluster: F39 (≠ Y302), C41 (= C304), R42 (≠ K305), G44 (= G307), C46 (= C309), C49 (= C312), C79 (= C342)

6yacN Plant psi-ferredoxin supercomplex (see paper)
47% identity, 23% coverage: 272:352/357 of query aligns to 8:87/97 of 6yacN

query
sites
6yacN

I

V

T

T

L

P

D

D

G

G

A

-

T

T

Q

Q

T

E

I

F

E

E

I

C

G

P

K

S

D

D

M

V

T

Y

L

I

L

L

D

D

A

H

A

A

L

E

E

E

N

V

A

G

M

I

D

D

A

L

P

P

Y
|
Y

A
x
S

C
|
C

K
x
R

A

A

G

G

V

S

C
|
C

S

S

T
x
S

C
|
C

R

A

C

G

K

K

V

V

L

V

E

G

G

G

E

E

V

V

E

D

M

Q

I

S

A

D

N

G

H

S

A

F

L

L

E

D

D

D

Y

E

E

Q

V

I

E

E

K

A

G

G

Y

F

V

V

L

L

S

T

C
|
C

Q

V

A

A

Y

Y

P

P

L

T

S

S

D

D

K

V

V

V

V

I

binding fe2/s2 (inorganic) cluster: Y37 (= Y302), S38 (≠ A303), C39 (= C304), R40 (≠ K305), C44 (= C309), S46 (≠ T311), C47 (= C312), C77 (= C342)

1awdA Ferredoxin [2fe-2s] oxidized form from chlorella fusca (see paper)
40% identity, 25% coverage: 269:357/357 of query aligns to 2:90/94 of 1awdA

query
sites
1awdA

K

K

A

V

A

T

I

L

T

K

L

T

D

P

G

S

A

G

T

E

Q

E

T

T

I

I

E

E

I

C

G

P

K

E

D

D

M

T

T

Y

L

I

L

L

D

D

A

A

A

A

L

E

E

E

N

A

A

G

M

L

D

D

A

L

P

P

Y
|
Y

A

S

C
|
C

K
x
R

A

A

G
|
G

V

A

C
|
C

S

S

T

S

C
|
C

R

A

C

G

K

K

V

V

L

E

E

S

G

G

E

E

V

V

E

D

M

Q

I

S

A

D

N

Q

H

S

A

F

L

L

E

D

D

D

Y

A

E

Q

V

M

E

G

K

K

G

G

Y

F

V

V

L

L

S

T

C
|
C

Q

V

A

A

Y

Y

P

P

L

T

S

S

D

D

K

V

V

T

V

I

V

L

D

T

Y

H

D

Q

Q

E

binding fe2/s2 (inorganic) cluster: Y35 (= Y302), C37 (= C304), R38 (≠ K305), G40 (= G307), C42 (= C309), C45 (= C312), C75 (= C342)

P56408 Ferredoxin from Scenedesmus fuscus (Green alga) (Chlorella fusca) (see paper)
40% identity, 25% coverage: 269:357/357 of query aligns to 2:90/94 of P56408

query
sites
P56408

K

K

A

V

A

T

I

L

T

K

L

T

D

P

G
x
S

A

G

T

E

Q

E

T

T

I

I

E

E

I

C

G

P

K

E

D

D

M

T

T

Y

L

I

L

L

D

D

A

A

A

A

L

E

E

E

N

A

A

G

M

L

D

D

A

L

P

P

Y

Y

A

S

C
|
C

K

R

A

A

G

G

V

A

C
|
C

S

S

T

S

C
|
C

R

A

C

G

K

K

V

V

L

E

E

S

G

G

E

E

V

V

E

D

M

Q

I

S

A

D

N

Q

H

S

A

F

L

L

E

D

D

D

Y

A

E

Q

V

M

E

G

K

K

G

G

Y

F

V

V

L

L

S

T

C
|
C

Q

V

A

A

Y

Y

P

P

L

T

S

S

D

D

K

V

V

T

V

I

V

L

D

T

Y

H

D

Q

Q

E

S9 (≠ G276) modified: Phosphoserine
C37 (= C304) binding
C42 (= C309) binding
C45 (= C312) binding
C75 (= C342) binding

P27787 Ferredoxin-1, chloroplastic; Ferredoxin I; Fd I from Zea mays (Maize) (see 2 papers)
41% identity, 23% coverage: 272:352/357 of query aligns to 60:139/150 of P27787

query
sites
P27787

I

I

T

T

L

P

D

E

G

G

A

E

T

V

Q

E

T

-

I

L

E

Q

I

V

G

P

K

D

D

D

M

V

T

Y

L

I

L

L

D

D

A

Q

A

A

L

E

E

E

N

D

A

G

M

I

D

D

A

L

P

P

Y

Y

A

S

C

C

K

R

A

A

G

G

V

S

C

C

S

S

T

S

C

C

R

A

C

G

K

K

V

V

L

V

E

S

G

G

E

S

V

V

E

D

M

Q

I

S

A

D

N

Q

H

S

A

Y

L

L

E
x
D

D

D

Y

G

E

Q

V

I

E

A

K

D

G

G

Y

W

V

V

L

L

S

T

C

C

Q

H

A

A

Y

Y

P

P

L

T

S

S

D

D

K

V

V

V

V

I

D117 (≠ E330) mutation to N: Decreased affinity for Fd-NADP(+) oxidoreductase and decreased electron-transfer activity.

Sites not aligning to the query:

1:52 modified: transit peptide, Chloroplast

5ogxA Crystal structure of amycolatopsis cytochrome p450 reductase gcob. (see paper)
27% identity, 67% coverage: 9:248/357 of query aligns to 104:333/333 of 5ogxA

query
sites
5ogxA

V

V

T

L

D

E

V

V

R

A

K

D

T

I

I

A

R

R

D

D

A

T

V

R

V

R

V

V

T

L

L

L

K

-

P

-

V

-

G

-

G

G

A

L

A

A

E

E

E

P

F

L

D

A

F

F

T

E

Q

A

G

G

Q

Q

Y
|
Y

L

V

T

E

F

L

R

V

R

-

D

-

F

V

D

P

G

G

E

S

E

G

L

A

R

R

R
|
R

S
x
Q

Y
|
Y

S
|
S

I

L

C

A

A

N

G

T

K

A

D

D

E

E

G

D

-

K

I

V

L

L

Q

E

V

L

G
x
H

I
x
V

K

R

R

R

V
|
V

D

P

G

G

G
|
G

-
x
V

A
|
A

F
x
T

S

D

T

G

W

W

A

L

N

F

T

D

E

G

L

L

K

A

A

A

G

G

D

D

T

R

L

V

Q

E

A

A

M

T

A

G

P

P

M

L

G

G

S

D

F

F

-

H

F

L

T

P

P

P

L

P

N

D

E

E

A

D

A

D

E

G

K

G

H

P

Y

M

L

V

G

L

F

I

A

G

G

G

G

G

S
x
T

G

G

I

L

T

A

P

P

V

L

L

V

S

G

I

I

L

A

K

R

T

T

T

A

L

L

A

A

A

R

E

H

P

P

N

S

A

R

S

E

F

V

T

L

L

L

V

Y

Y

H

A

G

N

V

K

R

G

G

V

A

N

A

T

D

I

L

M

Y

F

D

R

L

E

G

E

R

L

F

E

A

D

E

L

I

K

A

N

E

L

E

Y

H

M

P

G

G

R

-

F

F

N

R

L

F

I

V

H

P

V

V

L

L

E

-

S

S

D

D

A

E

Q

P

E

D

I

P

D

A

L

Y

F

R

T

G

G

G

L

F

V

P

T

T

E

D

E

A

K

-

C

-

A

-

Q

-

L

-

F

F

E

V

R

E

W

D

I

V

D

P

I

S

K

G

S

R

V

G

D

W

T

S

A

G

F

W

I

L

C

C

G

G

P

P

E

P

P

A

M

M

M

V

L

E

G

A

I

G

A

V

S

K

A

A

L

F

R

K

T

R

A

R

G

R

L

M

N

S

D

P

S

R

Q

R

I

I

K

H

F

R

E

E

L

K

F
|
F

A

T

S
x
P

A

A

Q
x
S

binding flavin-adenine dinucleotide: Y132 (= Y41), R144 (= R55), Q145 (≠ S56), Y146 (= Y57), S147 (= S58), H161 (≠ G71), V162 (≠ I72), V165 (= V75), G168 (= G78), V169 (vs. gap), A170 (= A79), T171 (≠ F80), T214 (≠ S122), F329 (= F244), P331 (≠ S246), S333 (≠ Q248)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 34, 35, 37, 39, 40, 42, 74

4fxcA Tertiary structure of [2fe-2s] ferredoxin from spirulina platensis refined at 2.5 angstroms resolution: structural comparisons of plant-type ferredoxins and an electrostatic potential analysis (see paper)
42% identity, 22% coverage: 275:352/357 of query aligns to 12:89/98 of 4fxcA

query
sites
4fxcA

D

E

G

G

A

I

T

N

Q

E

T

T

I

I

E

D

I

C

G

D

K

D

D

D

M

T

T

Y

L

I

L

L

D

D

A

A

A

A

L

E

E

E

N

A

A

G

M

L

D

D

A

L

P

P

Y
|
Y

A
x
S

C
|
C

K
x
R

A

A

G
|
G

V

A

C
|
C

S

S

T

T

C
|
C

R

A

C

G

K

T

V

I

L

T

E

S

G

G

E

T

V

I

E

D

M

Q

I

S

A

D

N

Q

H

S

A

F

L

L

E

D

D

D

Y

D

E

Q

V

I

E

E

K

A

G

G

Y

Y

V

V

L
|
L

S

T

C
|
C

Q

V

A

A

Y

Y

P

P

L

T

S

S

D

D

K

C

V

T

V

I

binding fe2/s2 (inorganic) cluster: Y39 (= Y302), S40 (≠ A303), C41 (= C304), R42 (≠ K305), G44 (= G307), C46 (= C309), C49 (= C312), L77 (= L340), C79 (= C342)

P0DPQ8 Aromatic O-demethylase, reductase subunit; NADH--hemoprotein reductase; EC 1.6.2.- from Amycolatopsis sp. (strain ATCC 39116 / 75iv2) (see paper)
27% identity, 67% coverage: 9:248/357 of query aligns to 105:334/334 of P0DPQ8

query
sites
P0DPQ8

V

V

T

L

D

E

V

V

R

A

K

D

T

I

I

A

R

R

D

D

A

T

V

R

V

R

V

V

T

L

L

L

K

-

P

-

V

-

G

-

G

G

A

L

A

A

E

E

E

P

F

L

D

A

F

F

T

E

Q

A

G

G

Q

Q

Y

Y

L

V

T

E

F

L

R

V

R

-

D

-

F

V

D

P

G

G

E

S

E

G

L

A

R

R

R
|
R

S
x
Q

Y
|
Y

S
|
S

I

L

C

A

A

N

G

T

K

A

D

D

E

E

G

D

-

K

I

V

L

L

Q

E

V

L

G
x
H

I
x
V

K
x
R

R

R

V

V

D

P

G

G

G

G

-
x
V

A
|
A

F
x
T

S

D

T

G

W

W

A

L

N

F

T

D

E

G

L

L

K

A

A

A

G

G

D

D

T

R

L

V

Q

E

A

A

M

T

A

G

P

P

M

L

G

G

S

D

F

F

-

H

F

L

T

P

P

P

L

P

N

D

E

E

A

D

A

D

E

G

K

G

H

P

Y

M

L

V

G

L

F

I

A

G

G

G

G

G

S
x
T

G

G

I

L

T

A

P

P

V

L

L

V

S

G

I

I

L

A

K

R

T

T

T

A

L

L

A

A

A

R

E

H

P

P

N

S

A

R

S

E

F

V

T

L

L

L

V

Y

Y

H

A

G

N

V

K

R

G

G

V

A

N

A

T

D

I

L

M

Y

F

D

R

L

E

G

E

R

L

F

E

A

D

E

L

I

K

A

N

E

L

E

Y

H

M

P

G

G

R

-

F

F

N

R

L

F

I

V

H

P

V

V

L

L

E

-

S

S

D

D

A

E

Q

P

E

D

I

P

D

A

L

Y

F

R

T

G

G

G

L

F

V

P

T

T

E

D

E

A

K

-

C

-

A

-

Q

-

L

-

F

F

E

V

R

E

W

D

I

V

D

P

I

S

K

G

S

R

V

G

D

W

T

S

A

G

F

W

I

L

C

C

G

G

P

P

E

P

P

A

M

M

M

V

L

E

G

A

I

G

A

V

S

K

A

A

L

F

R

K

T

R

A

R

G

R

L

M

N

S

D

P

S

R

Q

R

I

I

K

H

F

R

E

E

L

K

F
|
F

A

T

S

P

A

A

Q
x
S

RQYS 145:148 (≠ RSYS 55:58) binding
HVR 162:164 (≠ GIK 71:73) binding
VAT 170:172 (≠ -AF 79:80) binding
T215 (≠ S122) binding
F330 (= F244) binding
S334 (≠ Q248) binding

Sites not aligning to the query:

35 binding
40 binding
43 binding
75 binding

Query Sequence

>GFF393 FitnessBrowser__Phaeo:GFF393
MARFHDLVVTDVRKTIRDAVVVTLKPVGGAAEEFDFTQGQYLTFRRDFDGEELRRSYSIC
AGKDEGILQVGIKRVDGGAFSTWANTELKAGDTLQAMAPMGSFFTPLNEAAEKHYLGFAG
GSGITPVLSILKTTLAAEPNASFTLVYANKGVNTIMFREELEDLKNLYMGRFNLIHVLES
DAQEIDLFTGLVTEEKCAQLFERWIDIKSVDTAFICGPEPMMLGIASALRTAGLNDSQIK
FELFASAQPGRAKRKVTAGGTGTGANQTKAAITLDGATQTIEIGKDMTLLDAALENAMDA
PYACKAGVCSTCRCKVLEGEVEMIANHALEDYEVEKGYVLSCQAYPLSDKVVVDYDQ

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory