SitesBLAST

Q9BTZ2 Dehydrogenase/reductase SDR family member 4; NADPH-dependent carbonyl reductase; CR; NADPH-dependent retinol dehydrogenase/reductase; NRDR; humNRDR; Peroxisomal short-chain alcohol dehydrogenase; PSCD; SCAD-SRL; Short chain dehydrogenase/reductase family 25C member 2; Protein SDR25C2; Short-chain dehydrogenase/reductase family member 4; EC 1.1.1.184 from Homo sapiens (Human) (see 2 papers)
43% identity, 96% coverage: 8:251/255 of query aligns to 30:273/278 of Q9BTZ2

query
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Q9BTZ2

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S176 (≠ N154) Responsible for the stereoselective reduction of 3-ketosteroids into 3beta-hydroxysteroids and benzil into R-benzoin; mutation to F: Decreased reduction activity for benzil, isatin and retinal and increased activity for 5beta-Pregnane-3,20-dione and 5beta-Dihydrotestosterone. No change of stereoselectivity in 3-ketosteroids reduction and no change in 3beta-hydroxysteroid oxidation. Decreased reduction activity for isatin and increased activity for 5beta-Pregnane-3,20-dione, 5beta-Dihydrotestosterone, benzil and retinal; when associated with L-179. Change in stereoselective activity by the reduction of 5beta-Pregnane-3,20-dione predominantly to the 3alpha-hydroxysteroid; when associated with L-179. Switch from 3beta-hydroxysteroid to 3alpha-hydroxysteroid oxidation; when associated with L-179. Loss of cold catalytic inactivation; when associated with L-179 and N-195. Increased reduction activity for renital and oxidation activity for retinol; when associated with L-179 and N-195.
F179 (≠ I157) Responsible for the stereoselective reduction of 3-ketosteroids into 3beta-hydroxysteroids and benzil into R-benzoin; mutation to L: Decreased reduction activity for isatin and increased activity for 5beta-Pregnane-3,20-dione, 5beta-Dihydrotestosterone, benzil and retinal; when associated with F-176. Change in stereoselective activity by the reduction of 5beta-Pregnane-3,20-dione predominantly to the 3alpha-hydroxysteroid; when associated with F-176. Switch from 3beta-hydroxysteroid to 3alpha-hydroxysteroid oxidation; when associated with F-176. Loss of cold catalytic inactivation; when associated with F-176 and N-195. Increased reduction activity for renital and oxidation activity for retinol; when associated with F-176 and N-195.
T195 (≠ N173) mutation to N: Loss of cold catalytic inactivation. Loss of cold catalytic inactivation; when associated with F-176 and L-179. Switch in stereoselective activity from 3beta-hydroxysteroid to 3alpha-hydroxysteroid oxidation; when associated with F-176 and L-179. Increased reduction activity for renital and oxidation activity for retinol; when associated with F-176 and L-179.

Q8WNV7 Dehydrogenase/reductase SDR family member 4; NADPH-dependent carbonyl reductase; CR; PHCR; NADPH-dependent retinol dehydrogenase/reductase; NDRD; Peroxisomal carbonyl reductase; PerCR; Peroxisomal short-chain alcohol dehydrogenase; PSCD; Short chain dehydrogenase/reductase family 25C member 2; Protein SDR25C2; EC 1.1.1.184; EC 1.1.1.300 from Sus scrofa (Pig) (see 2 papers)
43% identity, 96% coverage: 8:251/255 of query aligns to 31:274/279 of Q8WNV7

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Q8WNV7

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37:61 (vs. 14:38, 56% identical) binding NADP(+)
F177 (≠ N154) Responsible for the stereoselective reduction of 3-ketosteroids into 3alpha-hydroxysteroids and benzil into S-benzoin; mutation to S: Change in stereoselective activity by the reduction of 3-ketosteroids and benzil into 3beta-hydroxysteroid and R-benzoin, respectively; when associated with F-180.
L180 (≠ I157) Responsible for the stereoselective reduction of 3-ketosteroids into 3alpha-hydroxysteroids and benzil into S-benzoin; mutation to F: Change in stereoselective activity by the reduction of 3-ketosteroids and benzil into 3beta-hydroxysteroid and R-benzoin, respectively; when associated with S-177.
Y183 (= Y160) active site, Proton acceptor
K187 (= K164) binding NADP(+)
N196 (= N173) Important for the maintenance of the quaternary structure, the catalytic activity and cold stability

Sites not aligning to the query:

277:279 Peroxisomal targeting signal

5ojiA Crystal structure of the dehydrogenase/reductase sdr family member 4 (dhrs4) from caenorhabditis elegans (see paper)
39% identity, 95% coverage: 10:251/255 of query aligns to 10:255/260 of 5ojiA

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active site: G21 (= G21), S148 (= S147), Y161 (= Y160), K165 (= K164)
binding isatin: S148 (= S147), S150 (≠ A149), Y161 (= Y160), V193 (≠ L192), S199 (≠ A198), L202 (= L201)
binding nadp nicotinamide-adenine-dinucleotide phosphate: A17 (≠ G17), T19 (= T19), I22 (= I22), S41 (= S41), R42 (= R42), N43 (≠ K43), N46 (≠ A46), I69 (≠ V68), N95 (= N94), H96 (≠ A95), G97 (≠ A96), N146 (≠ L145), S148 (= S147), Y161 (= Y160), K165 (= K164), G192 (= G191), I194 (= I193), T196 (= T195), M198 (≠ F197)

5ojgA Crystal structure of the dehydrogenase/reductase sdr family member 4 (dhrs4) from caenorhabditis elegans (see paper)
39% identity, 95% coverage: 10:251/255 of query aligns to 10:255/260 of 5ojgA

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active site: G21 (= G21), S148 (= S147), Y161 (= Y160), K165 (= K164)
binding butane-2,3-dione: S148 (= S147), Y161 (= Y160)
binding nadp nicotinamide-adenine-dinucleotide phosphate: A17 (≠ G17), T19 (= T19), G21 (= G21), I22 (= I22), S41 (= S41), R42 (= R42), N43 (≠ K43), N46 (≠ A46), I69 (≠ V68), N95 (= N94), H96 (≠ A95), G97 (≠ A96), N146 (≠ L145), S148 (= S147), Y161 (= Y160), K165 (= K164), P191 (= P190), I194 (= I193), T196 (= T195), M198 (≠ F197)

Q9S9W2 Short-chain dehydrogenase/reductase SDRA; Protein INDOLE-3-BUTYRIC ACID RESPONSE 1; Short-chain dehydrogenase/reductase A; EC 1.1.-.- from Arabidopsis thaliana (Mouse-ear cress) (see paper)
39% identity, 96% coverage: 8:251/255 of query aligns to 9:249/254 of Q9S9W2

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Q9S9W2

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K

A

E

Q

D

H

L

R

Q

R

N

N

L

L

V

V

K

E

K

K

T

T

N

V

E

Q

A

K

W

Y

G

G

S

K

I

I

D

D

V

I

L

V

V

V

C

C

N

N

A

A

T

A

N

N

P

P

V

S

Y

T

G

D

P

P

T

I

A

L

E

S

M

S

T

K

D

E

D

A

A

V

W

L

D

D

K

K

I

L

M

W

D

E

T

I

N

N

V

V

K

K

S

S

T

S

F

I

W

L

L

L

T

L

N

Q

M

D

V

M

L

A

P

P

Q

H

M

L

A

-

E

E

K

K

G

G

E

S

G

-

A
x
S

V

V

V

I

L

F

L

I

S

T

S

S

I

I

A

A

G

G

L

F

R

S

G

P

N

Q

T

G

V

A

I

M

G

A

T

M

Y

Y

G

G

V

V

S

T

K

K

A

T

A

A

E

L

A

L

A

G

L

L

A

T

R

K

N

A

L

L

A

A

V

A

E

E

W

M

G

A

P

P

K

D

G

-

I

T

R

R

I

V

N

N

S

A

I

V

A

A

P

P

G

G

L

F

I

V

K

P

T

T

D

H

F

F

A

A

R

S

A

F

L

I

W

T

E

G

D

S

P

S

E

E

R

V

A

R

K

E

Q

G

A

I

E

E

D

E

R

K

T

T

P

L

L

L

R

N

R

R

I

L

G

G

D

T

P

T

V

G

D

D

I

M

A

A

G

A

L

A

A

A

V

A

F

F

L

L

S

A

T

S

K

D

A

D

S

S

A

S

Y

Y

I

I

T

T

G

G

Q

E

V

T

I

L

V

V

A

V

D

A

G

G

R43 (= R42) mutation to C: In ibr1-1; resistance to the inhibitory effect of intermediate levels of indole-3-butyric acid (IBA) on root elongation.
S140 (≠ A141) mutation to F: In ibr1-8; resistance to the inhibitory effect of intermediate levels of indole-3-butyric acid (IBA) on root elongation.

A7DY56 Tropinone reductase; EC 1.1.1.206; EC 1.1.1.236 from Cochlearia officinalis (Common scurvygrass) (see paper)
33% identity, 97% coverage: 7:254/255 of query aligns to 15:262/273 of A7DY56

query
sites
A7DY56

D

S

M

L

T

E

G

G

K

M

V

T

A

A

V

L

I

V

T

T

G

G

S

G

T

S

K

K

G

G

I

I

G

G

R

E

A

A

I

V

A

V

G

E

E

E

M

L

A

A

V

M

C

L

G

G

A

A

K

R

V

V

V

H

I

T

S

C

S

A

R

R

K

D

A

E

E

T

A

Q

C

L

E

Q

Q

E

M

S

A

L

E

R

E

E

L

W

K

Q

A

A

Q

K

G

G

F

F

E

Q

A

V

M

T

A

T

I

S

P

V

C

C

H

D

V

V

G

S

R

S

K

R

E

D

D

Q

L

R

Q

E

N

K

L

L

V

M

K

E

K

T

T

V

N

S

E

S

A

L

W

F

-

Q

G

G

S

K

I

L

D

N

V

I

L

L

V

V

C

N

N

N

A

A

A

G

T

T

N

-

P

C

V

I

Y

T

G

K

P

P

T

T

A

I

E

D

M

Y

T

T

D

S

D

E

A

D

W

F

D

S

K

F

I

L

M

M

D

S

T

T

N

N

V

L

K

E

S

S

T

S

F

F

W

H

L

L

T

S

N

Q

M

L

V

A

L

H

P

P

Q

L

M

L

A

K

E

S

K

S

G

G

E

L

G

G

A

S

V

I

V

V

L

L

L

I

S

S

I

V

A

A

G

S

L

V

R

V

G

H

N

V

T

N

V

V

I

G

G

S

T

I

Y

Y

G

G

V

A

S

T

K

K

A

G

A

A

E

M

A

N

A

Q

L

L

A

A

R

R

N

N

L

L

A

A

V

C

E

E

W

W

G

A

P

S

K

D

G

S

I

I

R

K

I

V

N

N

S

S

I

V

A

C

P

P

G

G

L

F

I

I

K

S

T

T

D

P

F

L

A

A

R

S

A

N

L
x
Y

W

F

E

R

D

N

P

E

E

E

R

F

A

K

K

K

Q

E

A

V

E

E

D

N

R

I

T

I

P

P

L

T

R

G

R

R

I

V

G

G

D

E

P

A

V

N

D

E

I

V

A

S

G

S

L

L

A

V

V

A

F

Y

L

L

S

C

T

L

K

P

A

A

S

A

A

S

Y

Y

I

V

T

T

G

G

Q

Q

V

T

I

I

V

C

A

V

D

D

G

G

E

F

T

S

I

V

Y209 (≠ L201) mutation to S: Loss of tropinone or nortropinone reduction, but faster reduction of cyclohexanones.

1vl8B Crystal structure of gluconate 5-dehydrogenase (tm0441) from thermotoga maritima at 2.07 a resolution
35% identity, 98% coverage: 5:253/255 of query aligns to 1:250/252 of 1vl8B

query
sites
1vl8B

L

V

F

F

D

D

M

L

T

R

G

G

K

R

V

V

A

A

V

L

I

V

T

T

G
|
G

S

G

T

S

K
x
R

G
|
G

I
x
L

G

G

R

F

A

G

I

I

A

A

G

Q

E

G

M

L

A

A

V

E

C

A

G

G

A

C

K

S

V

V

I

V

S

A

S
|
S

R
|
R

K

N

A

L

E

E

A

E

C

A

E

S

Q

E

M

A

A

A

E

Q

E

K

L

L

-

T

K

E

A

K

Q

Y

G

G

F

V

E

E

A

T

M

M

A

A

I

F

P

R

C
|
C

H
x
D

V
|
V

G

S

R

N

K

Y

E

E

D

E

L

V

Q

K

N

K

L

L

V

L

K

E

K

A

T

V

N

K

E

E

A

K

W

F

G

G

S

K

I

L

D

D

V

T

L

V

V

V

C

N

N
x
A

A
|
A

A
x
G

T
x
I

N

N

P

R

V

R

Y

H

G

-

P

P

T

A

A

E

E

E

M

F

T

P

D

L

D

D

A

E

W

F

D

R

K

Q

I

V

M

I

D

E

T
x
V

N

N

V

L

K

F

S

G

T

T

F

Y

W

Y

L

V

T

C

N

R

M

E

V

A

L

F

P

S

Q

L

M

L

A

R

E

E

K

S

G

D

E

N

G

P

A

S

V

I

L

N

L
x
I

S

G

S
|
S

I

L

A

T

G

-

L

V

R

E

G

E

N

V

T

T

V

M

-

P

-

N

I
|
I

G

S

T

A

Y
|
Y

G

A

V

A

S

S

K
|
K

A

G

E

V

A

A

A

S

L

L

A

T

R

K

N

A

L

L

A

A

V

K

E

E

W

W

G

G

P

R

K

Y

G

G

I

I

R

R

I

V

N

N

S

V

I

I

A

A

P
|
P

G
|
G

L

W

I
x
Y

K

R

T
|
T

D

K

F
x
M

A
x
T

R

E

A

A

L

V

W

F

E

S

D

D

P

P

E

E

R

K

A

L

K

D

Q

Y

A

M

E

L

D

K

R

R

T

I

P

P

L

L

R

G

R

R

I

T

G

G

D

V

P

P

V

E

D

D

I

L

A

K

G

G

L

V

A

A

V

V

F

F

L

L

S

A

T

S

K

E

A

E

S

A

A

K

Y

Y

I

V

T

T

G

G

Q

Q

V

I

I

I

V

F

A

V

D

D

G

G

E

W

T

T

active site: G17 (= G21), S143 (= S147), I154 (= I157), Y157 (= Y160), K161 (= K164)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G13 (= G17), R16 (≠ K20), G17 (= G21), L18 (≠ I22), S37 (= S41), R38 (= R42), C63 (= C66), D64 (≠ H67), V65 (= V68), A91 (≠ N94), A92 (= A95), G93 (≠ A96), I94 (≠ T97), V114 (≠ T118), I141 (≠ L145), S143 (= S147), Y157 (= Y160), K161 (= K164), P187 (= P190), G188 (= G191), Y190 (≠ I193), T192 (= T195), M194 (≠ F197), T195 (≠ A198)

Q9ZW19 Tropinone reductase homolog At2g29360; EC 1.1.1.- from Arabidopsis thaliana (Mouse-ear cress) (see paper)
33% identity, 99% coverage: 2:254/255 of query aligns to 10:262/271 of Q9ZW19

query
sites
Q9ZW19

N

D

N

K

P

P

L

R

F

W

D

S

M

L

T

V

G

G

K

M

V

T

A

A

V

L

I

V

T

T

G

G

S

G

T

S

K

K

G

G

I

I

G

G

R

E

A

A

I

V

A

V

G

E

E

E

M

L

A

A

V

T

C

L

G

G

A

A

K

R

V

I

V

H

I

T

S

C

S

A

R

R

K

D

A

E

E

T

A

Q

C

L

E

Q

Q

E

M

S

A

L

E

R

E

K

L

W

K

Q

A

A

Q

K

G

G

F

F

E

Q

A

V

M

T

A

T

I

S

P

V

C

C

H

D

V

V

G

S

R

S

K

R

E

D

D

K

L

R

Q

E

N

K

L

L

V

M

K

E

K

T

T

V

N

S

E

T

A

I

W

F

-

E

G

G

S

K

I

L

D

N

V

I

L

L

V

V

C

N

N

N

A

V

A

G

T

T

N

C

P

-

V

I

Y

V

G

K

P

P

T

T

A

L

E

Q

M

H

T

T

D

A

D

E

A

D

W

F

D

S

K

F

I

T

M

M

D

A

T

T

N

N

V

L

K

E

S

S

T

A

F

F

W

H

L

L

T

S

N

Q

M

L

V

A

L

H

P

P

Q

L

M

L

A

K

E

A

K

S

G

G

E

S

G

G

A

S

V

I

V

V

L

L

L

I

S

S

I

V

A

S

G

G

L

V

R

V

G

H

N

V

T

N

V

G

I

A

G

S

T

I

Y

Y

G

G

V

V

S

S

K

K

A

G

A

A

E

M

A

N

A

Q

L

L

A

G

R

R

N

N

L

L

A

A

V

C

E

E

W

W

G

A

P

S

K

D

G

N

I

I

R

R

I

T

N

N

S

S

I

V

A

C

P

P

G

W

L

F

I

I

K

E

T

T

D

P

F

L

A

V

R

T

A

E

L
x
S

W

L

E

S

D

N

P

E

E

E

R

F

A

R

K

K

Q

E

A

V

E

E

D

S

R

R

T

P

P

P

L

M

R

G

R

R

I

V

G

G

D

E

P

V

V

N

D

E

I

V

A

S

G

S

L

L

A

V

V

A

F

F

L

L

S

C

T

L

K

P

A

A

S

A

A

S

Y

Y

I

I

T

T

G

G

Q

Q

V

T

I

I

V

C

A

V

D

D

G

G

E

F

T

T

I

V

S209 (≠ L201) mutation to Y: Loss of activity with quinuclidinone and decreased activity with cyclohexanones.

4fn4A Short-chain NAD(h)-dependent dehydrogenase/reductase from sulfolobus acidocaldarius (see paper)
32% identity, 97% coverage: 8:255/255 of query aligns to 5:254/254 of 4fn4A

query
sites
4fn4A

M

L

T

K

G

N

K

K

V

V

A

V

V

I

I

V

T

T

G
|
G

S

A

T

G

K
x
S

G
|
G

I
|
I

G

G

R

R

A

A

I

I

A

A

G

K

E

K

M

F

A

A

V

L

C

N

G

D

A

S

K

I

V

V

I

A

S

V

S
x
E

R
x
L

K

L

A

E

E

D

A
x
R

C

L

E

N

Q

Q

M

I

A

V

E

Q

E

E

L

L

K

R

A

G

Q

M

G

G

F

K

E

E

A

V

M

L

A

G

I

V

P

K

C
x
A

H
x
D

V
|
V

G

S

R

K

K

K

E

K

D

D

L

V

Q

E

N

E

L

F

V

V

K

R

T

T

N

F

E

E

A

T

W

Y

G

S

S

R

I

I

D

D

V

V

L

L

V

C

C

N

N
|
N

A

A

A
x
G

T
x
I

N

M

P

D

V

G

Y

V

G

T

P

P

T

V

A

A

E

E

M

V

T

S

D

D

D

E

A

L

W

W

D

E

K

R

I

V

M

L

D

A

T

V

N

N

V

L

K

Y

S

S

T

A

F

F

W

Y

L

S

T

S

N

R

M

A

V

V

L

I

P

P

Q

I

M

M

A

L

E

K

K

Q

G

G

E

K

G

G

A

V

V

I

V

V

L

N

L
x
T

S

A

S
|
S

I

I

A

A

G

G

L

I

R

R

G

G

N

G

T

F

V

A

I

G

G

A

T

P

Y
|
Y

G

T

V

V

S

A

K
|
K

A

H

A

G

E

L

A

I

A

G

L

L

A

T

R

R

N

S

L

I

A

A

V

A

E

H

W

Y

G

G

P

D

K

Q

G

G

I

I

R

R

I

A

N

V

S

A

I

V

A

L

P
|
P

G

G

L

T

I
x
V

K

K

T
|
T

D
x
N

F
x
I

A

G

R

L

A

G

L

S

W

S

E

K

D

P

P
x
S

E

E

R

L

A

G

K

M

Q

R

A

T

E

L

D

T

R

K

-

L

-

M

T

S

P

L

L

S

R

S

R

R

I

L

G

A

D

E

P

P

V

E

D

D

I

I

A

A

G

N

L

V

A

I

V

V

F

F

L

L

S

A

T

S

K

D

A

E

S

A

A

S

Y

F

I

V

T

N

G

G

Q

D

V

A

I

V

V

V

A

V

D

D

G

G

E

L

T

T

I

V

C

L

active site: G18 (= G21), S144 (= S147), Y157 (= Y160), K161 (= K164), S202 (≠ P205)
binding nicotinamide-adenine-dinucleotide: G14 (= G17), S17 (≠ K20), G18 (= G21), I19 (= I22), E38 (≠ S41), L39 (≠ R42), R43 (≠ A46), A63 (≠ C66), D64 (≠ H67), V65 (= V68), N91 (= N94), G93 (≠ A96), I94 (≠ T97), T142 (≠ L145), S144 (= S147), Y157 (= Y160), K161 (= K164), P187 (= P190), V190 (≠ I193), T192 (= T195), N193 (≠ D196), I194 (≠ F197)

1g6kA Crystal structure of glucose dehydrogenase mutant e96a complexed with NAD+
36% identity, 97% coverage: 7:253/255 of query aligns to 4:251/261 of 1g6kA

query
sites
1g6kA

D

D

M

L

T

E

G

G

K

K

V

V

A

V

V

V

I

I

T

T

G

G

S

S

T

S

K
x
T

G
|
G

I
x
L

G

G

R

K

A

S

I

M

A

A

G

I

E

R

M

F

A

A

V

T

C

E

G

K

A

A

K

K

V

V

I

V

S

N

S

Y

R
|
R

-

S

K

K

A

E

E

D

A

E

C

A

E

N

Q

S

M

V

A

L

E

E

L

I

K

K

A

K

Q

V

G

G

F

G

E

E

A

A

M

I

A

A

I

V

P

K

C

G

H
x
D

V
|
V

G

T

R

V

K

E

E

S

D

D

L

V

Q

I

N

N

L

L

V

V

K

Q

K

S

T

A

N

I

E

K

A

E

W

F

G

G

S

K

I

L

D

D

V

V

L

M

V

I

C

N

N
|
N

A
|
A

A
x
G

-

L

T

A

N

N

P

P

V

V

Y

-

G

-

P

S

T

S

A

H

E

E

M

M

T

S

D

L

D

S

A

D

W

W

D

N

K

K

I

V

M

I

D

D

T

T

N

N

V

L

K

T

S

G

T

A

F

F

W

L

L

G

T

S

N

R

M

E

V

A

L

I

P

K

Q

Y

M

F

A

V

E

E

K

N

G

D

-

I

E

K

G

G

A

T

V

V

V

I

L

N

L
x
M

S

S

S
|
S

I

V

A

H

G

E

L

K

R

I

G

P

N

W

T

P

V

L

I

F

G

V

T

H

Y
|
Y

G

A

V

A

S

S

K
|
K

A

G

E

M

A

K

A

L

L

M

A

T

R

E

N

T

L

L

A

A

V

L

E

E

W

Y

G

A

P

P

K

K

G

G

I

I

R

R

I

V

N

N

S

N

I

I

A

G

P
|
P

G
|
G

L

A

I
|
I

K

N

T
|
T

D

P

F

I

A

N

R

A

A

E

L

K

W

F

E

A

D

D

P

P

E

E

R

Q

A

R

K

A

Q

D

A

V

E

E

D

S

R

M

T

I

P

P

L

M

R

G

R

Y

I

I

G

G

D

E

P

P

V

E

D

E

I

I

A

A

G

A

L

V

A

A

V

A

F

W

L

L

S

A

T

S

K

S

A

E

S

A

A

S

Y

Y

I

V

T

T

G

G

Q

I

V

T

I

L

V

F

A

A

D

D

G

G

E

M

T

T

active site: G18 (= G21), S145 (= S147), Y158 (= Y160), K162 (= K164)
binding nicotinamide-adenine-dinucleotide: T17 (≠ K20), G18 (= G21), L19 (≠ I22), R39 (= R42), D65 (≠ H67), V66 (= V68), N92 (= N94), A93 (= A95), G94 (≠ A96), M143 (≠ L145), S145 (= S147), Y158 (= Y160), P188 (= P190), G189 (= G191), I191 (= I193), T193 (= T195)

P40288 Glucose 1-dehydrogenase; EC 1.1.1.47 from Priestia megaterium (Bacillus megaterium) (see 2 papers)
36% identity, 97% coverage: 7:253/255 of query aligns to 4:251/261 of P40288

query
sites
P40288

D

D

M

L

T

E

G

G

K

K

V

V

A

V

V
|
V

I
|
I

T
|
T

G
|
G

S
|
S

T
x
S

K
x
T

G
|
G

I
x
L

G
|
G

R
x
K

A
x
S

I
x
M

A
|
A

G
x
I

E
x
R

M
x
F

A
|
A

V
x
T

C
x
E

G
x
K

A
|
A

K
|
K

V
|
V

I

V

S

N

S

Y

R

R

-

S

K

K

A

E

E

D

A

E

C

A

E

N

Q

S

M

V

A

L

E

E

L

I

K

K

A

K

Q

V

G

G

F

G

E

E

A

A

M

I

A

A

I

V

P

K

C

G

H

D

V

V

G

T

R

V

K

E

E

S

D

D

L

V

Q

I

N

N

L

L

V

V

K

Q

K

S

T

A

N

I

E

K

A

E

W

F

G

G

S

K

I

L

D

D

V

V

L

M

V

I

C

N

N

N

A

A

A

G

-

L

T
x
E

N

N

P

P

V

V

Y

-

G

-

P

S

T

S

A

H

E

E

M

M

T

S

D

L

D

S

A
x
D

W

W

D

N

K

K

I
x
V

M

I

D

D

T

T

N

N

V

L

K

T

S

G

T

A

F

F

W

L

L

G

T

S

N

R

M

E

V

A

L

I

P

K

Q

Y

M

F

A

V

E
|
E

K

N

G

D

-

I

E

K

G

G

A

T

V

V

V

I

L

N

L

M

S

S

I

V

A

H

G

E

L

K

R

I

G

P

N

W

T

P

V

L

I

F

G

V

T

H

Y

Y

G

A

V

A

S

S

K

K

A

G

E

M

A

K

A

L

L

M

A

T

R

E

N

T

L

L

A

A

V

L

E

E

W

Y

G

A

P

P

K

K

G

G

I

I

R

R

I
x
V

N

N

S

N

I

I

A

G

P

P

G

G

L

A

I

I

K

N

T

T

D
x
P

F

I

A

N

R

A

A

E

L

K

W

F

E

A

D

D

P

P

E

E

R

Q

A

R

K

A

Q

D

A

V

E
|
E

D

S

R

M

T

I

P

P

L

M

R

G

R
x
Y

I

I

G

G

D

E

P

P

V

E

D

E

I

I

A

A

G

A

L

V

A

A

V

A

F

W

L

L

S

A

T

S

K

S

A

E

S

A

A

S

Y

Y

I

V

T

T

G

G

Q

I

V

T

I

L

V

F

A

A

D

D

G

G

E

M

T

T

11:35 (vs. 14:38, 52% identical) binding NADP(+)
E96 (≠ T97) mutation E->A,G,K: Heat stable.
D108 (≠ A111) mutation to N: Heat stable.
V112 (≠ I115) mutation to A: Heat stable.
E133 (= E136) mutation to K: Heat stable.
V183 (≠ I185) mutation to I: Heat stable.
P194 (≠ D196) mutation to Q: Heat stable.
E210 (= E212) mutation to K: Heat stable.
Y217 (≠ R219) mutation to H: Heat stable.

Sites not aligning to the query:

252 Q→L: Heat stable.
253 Y→C: Heat stable.
258 A→G: Heat stable.

4jroC Crystal structure of 3-oxoacyl-[acyl-carrier protein]reductase (fabg) from listeria monocytogenes in complex with NADP+
37% identity, 96% coverage: 8:251/255 of query aligns to 3:244/247 of 4jroC

query
sites
4jroC

M

L

T

Q

G

G

K

K

V

V

A

A

V

V

I

V

T

T

G
|
G

S

G

T
x
S

K
x
R

G
|
G

I
|
I

G

G

R

R

A

D

I

I

A

A

G

I

E

N

M

L

A

A

V

K

C

E

G

G

A

A

K

N

V

I

V

F

I

F

S
x
N

S
x
Y

R
x
N

K
x
G

A
x
S

-

P

E

E

A

A

C

A

E

E

Q

E

M

T

A

A

E

K

E

L

L

V

K

A

A

E

Q

H

G

G

F

V

E

E

A

V

M

E

A

A

I

M

P

K

C

A

H
x
N

V
|
V

G

A

R

I

K

A

E

E

D

D

L

V

Q

D

N

A

L

F

V

F

K

K

K

Q

T

A

N

I

E

E

A

R

W

F

G

G

S

R

I

V

D

D

V

I

L

L

V

V

C

N

N
|
N

A
|
A

A

G

T
x
I

N

T

P

R

V

-

Y

D

G

N

P

L

T

L

A

M

E

R

M

M

T

K

D

E

D

D

A

E

W

W

D

D

K

D

I

V

M

I

D

N

T
x
I

N

N

V

L

K

K

S

G

T

T

F

F

W

L

L

C

T

T

N

K

M

A

V

V

L

S

P

R

Q

T

M

M

A

M

E

K

K

Q

G

R

E

A

G

G

A

K

V

I

L

N

L

M

S

A

S
|
S

I

V

A

V

G

G

L

L

R

I

G

G

N

N

T

A

V

G

I
x
Q

G

A

T

N

Y
|
Y

G

V

V

A

S

S

K
|
K

A

A

A

G

E

V

A

I

A

G

L

L

A

T

R

K

N

T

L

T

A

A

V

R

E

E

W

L

G

A

P

P

K

R

G

G

I

I

R

N

I

V

N

N

S

A

I

V

A

A

P
|
P

G

G

L

F

I
|
I

K

T

T
|
T

D

D

F

M

A

T

R

D

A

K

L

L

W

D

E

E

D

K

P

T

E

K

R

E

A

A

K

M

Q

L

A

A

E

Q

D

-

R

-

T

I

P

P

L

L

R

G

R

A

I

Y

G

G

D

T

P

T

V

E

D

D

I

I

A

A

G

N

L

A

A

V

V

L

F

F

L

L

S

A

T

S

K

D

A

A

S

S

A

K

Y

Y

I

I

T

T

G

G

Q

Q

V

T

I

L

V

S

A

V

D

D

G

G

active site: G16 (= G21), S142 (= S147), Q152 (≠ I157), Y155 (= Y160), K159 (= K164)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G17), S14 (≠ T19), R15 (≠ K20), G16 (= G21), I17 (= I22), N35 (≠ S40), Y36 (≠ S41), N37 (≠ R42), G38 (≠ K43), S39 (≠ A44), N63 (≠ H67), V64 (= V68), N90 (= N94), A91 (= A95), I93 (≠ T97), I113 (≠ T118), S142 (= S147), Y155 (= Y160), K159 (= K164), P185 (= P190), I188 (= I193), T190 (= T195)

3osuA Crystal structure of the 3-oxoacyl-acyl carrier protein reductase, fabg, from staphylococcus aureus
36% identity, 95% coverage: 11:251/255 of query aligns to 5:243/246 of 3osuA

query
sites
3osuA

K

K

V

S

A

A

V

L

I

V

T

T

G

G

S

A

T

S

K

R

G
|
G

I

I

G

G

R

R

A

S

I

I

A

A

G

L

E

Q

M

L

A

A

V

E

C

E

G

G

A

Y

K

N

V

V

V

A

I

V

S

N

-

Y

S

A

R

G

K

S

A

K

E

E

A

K

C

A

E

E

Q

A

M

V

A

V

E

E

L

I

K

K

A

A

Q

K

G

G

F

V

E

D

A

S

M

F

A

A

I

I

P

Q

C

A

H

N

V

V

G

A

R

D

K

A

E

D

D

E

L

V

Q

K

N

A

L

M

V

I

K

K

K

E

T

V

N

V

E

S

A

Q

W

F

G

G

S

S

I

L

D

D

V

V

L

L

V

V

C

N

N
|
N

A

A

A

G

T

I

N

T

P

R

V

-

Y

D

G

N

P

L

T

L

A

M

E

R

M

M

T

K

D

E

D

Q

A

E

W

W

D

D

K

D

I

V

M

I

D

D

T

T

N

N

V

L

K

K

S

G

T

V

F

F

W

N

L

C

T

I

N

Q

M

K

V

A

L

T

P

P

Q

Q

M

M

A

L

E

R

K

Q

G

R

E

S

G

G

A

A

V

I

L

N

L

L

S

S

S
|
S

I

V

A

V

G

G

L

A

R

V

G

G

N

N

T

P

V

G

I
x
Q

G

A

T

N

Y
|
Y

G

V

V

A

S

T

K
|
K

A

A

A

G

E

V

A

I

A

G

L

L

A

T

R

K

N

S

L

A

A

A

V

R

E

E

W

L

G

A

P

S

K

R

G

G

I

I

R

T

I

V

N

N

S

A

I

V

A

A

P

P

G

G

L

F

I

I

K

V

T

S

D

D

F

M

A

T

R

D

A

A

L

L

W

S

E

D

D

-

P

-

E

E

R

L

A

K

K

E

Q

Q

A

M

E

L

D

T

R

Q

T

I

P

P

L

L

R

A

R

R

I

F

G

G

D

Q

P

D

V

T

D

D

I

I

A

A

G

N

L

T

A

V

V

A

F

F

L

L

S

A

T

S

K

D

A

K

S

A

A

K

Y

Y

I

I

T

T

G

G

Q

Q

V

T

I

I

V

H

A

V

D

N

G

G

active site: G15 (= G21), S141 (= S147), Q151 (≠ I157), Y154 (= Y160), K158 (= K164)
binding magnesium ion: N89 (= N94), K158 (= K164)

8w0oA Gdh-105 crystal structure
35% identity, 98% coverage: 7:255/255 of query aligns to 4:253/259 of 8w0oA

query
sites
8w0oA

D

D

M

L

T

K

G

G

K

K

V

V

A

V

V

A

I

I

T

T

G
|
G

S

A

T

A

K
x
S

G
|
G

I
x
L

G

G

R

K

A

A

I

M

A

A

G

I

E

R

M

F

A

G

V

K

C

E

G

Q

A

A

K

K

V

V

I

I

S

N

-

Y

-
x
Y

S

S

R

N

K

K

A
x
Q

E

D

A

P

C

N

E

E

Q

V

M

K

A

E

E

E

E

V

L

I

K

K

A

A

Q

G

G

G

F

-

E

E

A

A

M

V

A

V

I

V

P

Q

C

G

H
x
D

V
|
V

G

T

R

K

K

E

E

E

D

D

L

V

Q

K

N

N

L

I

V

V

K

Q

K

T

T

A

N

I

E

K

A

E

W

F

G

G

S

T

I

L

D

D

V

I

L

M

V

I

C

N

N
|
N

A
|
A

A
x
G

-

L

T

E

N

N

P

P

V

V

Y

-

G

-

P

P

T

S

A

H

E

E

M

M

T

P

D

L

D

K

A

D

W

W

D

D

K

K

I

V

M

I

D

G

T

T

N

N

V

L

K

T

S

G

T

A

F

F

W

L

L

G

T

S

N

R

M

E

V

A

L

I

P

K

Q

Y

M

F

A

V

E

E

K

N

G

D

-

I

E

K

G

G

A

N

V

V

V

I

L

N

L
x
M

S

S

I

V

A

H

G

E

L

V

R

I

G

P

N

W

T

P

V

L

I

F

G

V

T

H

Y
|
Y

G

A

V

A

S

S

K
|
K

A

G

E

M

A

K

A

L

L

M

A

T

R

K

N

T

L

L

A

A

V

L

E

E

W

Y

G

A

P

P

K

K

G

G

I

I

R

R

I

V

N

N

S

N

I

I

A

G

P
|
P

G
|
G

L

A

I
|
I

K

N

T
|
T

D

T

F

I

A

N

R

A

A

E

L

K

W

F

E

A

D

D

P

P

E

K

R

Q

A

K

K

A

Q

D

A

V

E

E

D

S

R

M

T

I

P

P

L

M

R

G

R

Y

I

I

G

G

D

E

P

P

V

E

D

E

I

I

A

A

G

A

L

V

A

A

V

A

F

W

L

L

S

A

T

S

K

K

A

E

S

A

A

S

Y

Y

I

V

T

T

G

G

Q

I

V

T

I

L

V

F

A

A

D

D

G

G

E

M

T

T

I

L

C

Y

binding nicotinamide-adenine-dinucleotide: G14 (= G17), S17 (≠ K20), G18 (= G21), L19 (≠ I22), Y39 (vs. gap), Q43 (≠ A44), D65 (≠ H67), V66 (= V68), N92 (= N94), A93 (= A95), G94 (≠ A96), M143 (≠ L145), Y158 (= Y160), K162 (= K164), P188 (= P190), G189 (= G191), I191 (= I193), T193 (= T195)

8hsaA Brucella melitensis 7-alpha-hydroxysteroid dehydrogenase mutant: 1-53 truncation/m196i/i258m/k262t-NAD+
40% identity, 96% coverage: 6:251/255 of query aligns to 3:245/248 of 8hsaA

query
sites
8hsaA

F

F

D

H

M

L

T

N

G

D

K

A

V

V

A

A

V

I

I

V

T

T

G
|
G

S

A

T

A

K

A

G

G

I
|
I

G

G

R

R

A

A

I

I

A

A

G

G

E

T

M

F

A

A

V

K

C

A

G

G

A

A

K

S

V

V

I

V

S

T

S
x
D

R
x
L

K

K

A

S

E

E

A

G

C

A

E

E

Q

A

M

V

A

A

E

A

L

I

K

R

A

Q

Q

A

G

G

F

G

E

K

A

A

M

I

A

G

I

L

P

E

C
|
C

H
x
N

V
|
V

G

T

R

D

K

E

E

Q

D

H

L

R

Q

E

N

A

L

V

V

I

K

K

K

A

T

A

N

L

E

D

A

Q

W

F

G

G

S

K

I

I

D

T

V

V

L

L

V

V

C

N

N
|
N

A
|
A

A
x
G

T

G

N

G

P

G

V

P

Y

K

G

P

P

F

T

D

A

M

E

P

M

M

T

S

D

D

D

F

A

E

W

W

D

-

K

-

I

A

M

F

D

K

T

L

N

N

V

L

K

F

S

S

T

L

F

F

W

R

L

L

T

S

N

Q

M

L

V

A

L

A

P

P

Q

H

M

M

A

Q

E

K

K

A

G

G

E

G

G

G

A

A

V

I

V

L

L

N

L
x
I

S
|
S

S

S

I

I

A

A

G

G

L

E

R

N

G

T

N

N

T

V

V

R

I

M

G

A

T

S

Y
|
Y

G

G

V

S

S

S

K
|
K

A

A

E

V

A

N

A

H

L

L

A

T

R

R

N

N

L

I

A

A

V

F

E

D

W

V

G

G

P

P

K

M

G

G

I

I

R

R

I

V

N

N

S

A

I

I

A

A

P
|
P

G
|
G

L

A

I

M

K

K

T

T

D

D

F

-

A

A

R

L

A

A

L

T

W

V

E

L

D

T

P

P

E

E

R

I

A

E

K

R

Q

A

A

M

E

L

D

K

R

H

T

T

P

P

L

L

R

G

R

R

I

L

G

G

D

E

P

A

V

Q

D

D

I

I

A

A

G

N

L

A

A

A

V

L

F

F

L

L

S

C

T

S

K

P

A

A

S

A

A

A

Y

W

I

I

T

S

G

G

Q

Q

V

V

I

L

V

T

A

V

D

S

G

G

binding nicotinamide-adenine-dinucleotide: G14 (= G17), I19 (= I22), D38 (≠ S41), L39 (≠ R42), C63 (= C66), N64 (≠ H67), V65 (= V68), N91 (= N94), A92 (= A95), G93 (≠ A96), I140 (≠ L145), S141 (= S146), Y155 (= Y160), K159 (= K164), P185 (= P190), G186 (= G191)

2ae2A Tropinone reductase-ii complexed with NADP+ and pseudotropine (see paper)
32% identity, 96% coverage: 6:251/255 of query aligns to 4:252/259 of 2ae2A

query
sites
2ae2A

F

W

D

N

M

L

T

E

G

G

K

C

V

T

A

A

V

L

I

V

T

T

G
|
G

S

G

T
x
S

K
x
R

G
|
G

I
|
I

G

G

R

Y

A

G

I

I

A

V

G

E

E

E

M

L

A

A

V

S

C

L

G

G

A

A

K

S

V

V

V

Y

I

T

S

C

S
|
S

R
|
R

K

N

A

Q

E

K

A

E

C

L

E

N

Q

D

M

C

A

L

E

T

E

Q

L

W

K

R

A

S

Q

K

G

G

F

F

E

K

A

V

M

E

A

A

I

S

P

V

C
|
C

H

D

V
x
L

G

S

R

S

K

R

E

S

D

E

L

R

Q

Q

N

E

L

L

V

M

K

N

K

T

T

V

-

A

N

N

E

H

A

F

W

H

G

G

S

K

I

L

D

N

V

I

L

L

V

V

C

N

N
|
N

A

A

A
x
G

T

I

N

-

P

V

V

I

Y

Y

G

K

P

E

T

A

A

K

E

D

M

Y

T

T

D

V

D

E

A

D

W

Y

D

S

K

L

I

I

M

M

D

S

T
x
I

N

N

V

F

K

E

S

A

T

A

F

Y

W

H

L

L

T

S

N

V

M

L

V

A

L

H

P

P

Q

F

M

L

A

K

E

A

K

S

G

E

E

R

G

G

A

N

V

V

L

F

L
x
I

S

S

S
|
S

I

V

A

S

G

G

L

A

R

L

G

A

N

V

T

P

V

Y

I
x
E

G

A

T

V

Y
|
Y

G

G

V

A

S

T

K
|
K

A

G

A

A

E

M

A

D

A

Q

L

L

A

T

R

R

N

C

L

L

A

A

V

F

E

E

W

W

G

A

P

K

K

D

G

N

I

I

R

R

I

V

N

N

S

G

I

V

A

G

P
|
P

G
|
G

L
x
V

I
|
I

K

A

T
|
T

D
x
S

F
x
L

A
x
V

R

E

A

M

L

T

W

I

E

Q

D

D

P

P

E

E

R

Q

A

K

K

E

Q

N

A

L

E

N

-

K

-

L

-

I

D

D

R

R

T

C

P

A

L

L

R

R

I

M

G

G

D

E

P

P

V

K

D

E

I

L

A

A

G

A

L

M

A

V

V

A

F

F

L

L

S

C

T

F

K

P

A

A

S

A

A

S

Y

Y

I

V

T

T

G

G

Q

Q

V

I

I

I

V

Y

A

V

D

D

G

G

active site: G19 (= G21), S145 (= S147), Y158 (= Y160), K162 (= K164)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G15 (= G17), S17 (≠ T19), R18 (≠ K20), G19 (= G21), I20 (= I22), S39 (= S41), R40 (= R42), C64 (= C66), L66 (≠ V68), N93 (= N94), G95 (≠ A96), I116 (≠ T118), I143 (≠ L145), S145 (= S147), Y158 (= Y160), K162 (= K164), P188 (= P190), G189 (= G191), V190 (≠ L192), I191 (= I193), T193 (= T195), S194 (≠ D196), L195 (≠ F197), V196 (≠ A198)
binding pseudotropine: S145 (= S147), E155 (≠ I157), Y158 (= Y160), L195 (≠ F197)

1ipfA Tropinone reductase-ii complexed with NADPH and tropinone (see paper)
32% identity, 96% coverage: 6:251/255 of query aligns to 4:252/259 of 1ipfA

query
sites
1ipfA

F

W

D

N

M

L

T

E

G

G

K

C

V

T

A

A

V

L

I

V

T

T

G
|
G

S

G

T

S

K
x
R

G
|
G

I
|
I

G

G

R

Y

A

G

I

I

A

V

G

E

E

E

M

L

A

A

V

S

C

L

G

G

A

A

K

S

V

V

V

Y

I

T

S

C

S
|
S

R
|
R

K

N

A

Q

E

K

A

E

C

L

E

N

Q

D

M

C

A

L

E

T

E

Q

L

W

K

R

A

S

Q

K

G

G

F

F

E

K

A

V

M

E

A

A

I

S

P

V

C
|
C

H
x
D

V
x
L

G

S

R

S

K

R

E

S

D

E

L

R

Q

Q

N

E

L

L

V

M

K

N

K

T

T

V

-

A

N

N

E

H

A

F

W

H

G

G

S

K

I

L

D

N

V

I

L

L

V

V

C

N

N
|
N

A

A

A

G

T

I

N

-

P

V

V

I

Y

Y

G

K

P

E

T

A

A

K

E

D

M

Y

T

T

D

V

D

E

A

D

W

Y

D

S

K

L

I

I

M

M

D

S

T

I

N

N

V

F

K

E

S

A

T

A

F

Y

W

H

L

L

T

S

N

V

M

L

V

A

L

H

P

P

Q

F

M

L

A

K

E

A

K

S

G

E

E

R

G

G

A

N

V

V

L

F

L

I

S

S

S
|
S

I

V

A
x
S

G

G

L

A

R

L

G

A

N

V

T

P

V

Y

I
x
E

G

A

T

V

Y
|
Y

G

G

V

A

S

T

K
|
K

A

G

A

A

E

M

A

D

A

Q

L

L

A

T

R

R

N

C

L

L

A

A

V

F

E

E

W

W

G

A

P

K

K

D

G

N

I

I

R

R

I

V

N

N

S

G

I

V

A

G

P
|
P

G

G

L
x
V

I
|
I

K

A

T
|
T

D
x
S

F
x
L

A
x
V

R

E

A

M

L

T

W

I

E

Q

D

D

P

P

E

E

R

Q

A

K

K

E

Q

N

A

L

E

N

-

K

-

L

-

I

D

D

R

R

T

C

P

A

L

L

R

R

I

M

G

G

D

E

P

P

V

K

D

E

I

L

A

A

G

A

L

M

A

V

V

A

F

F

L

L

S

C

T

F

K

P

A

A

S

A

A

S

Y

Y

I

V

T

T

G

G

Q

Q

V

I

I

I

V

Y

A

V

D

D

G

G

active site: G19 (= G21), S145 (= S147), Y158 (= Y160), K162 (= K164)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G15 (= G17), R18 (≠ K20), G19 (= G21), I20 (= I22), S39 (= S41), R40 (= R42), C64 (= C66), D65 (≠ H67), L66 (≠ V68), N93 (= N94), S145 (= S147), Y158 (= Y160), K162 (= K164), P188 (= P190), V190 (≠ L192), I191 (= I193), T193 (= T195), S194 (≠ D196), L195 (≠ F197), V196 (≠ A198)
binding 8-methyl-8-azabicyclo[3,2,1]octan-3-one: S147 (≠ A149), E155 (≠ I157), Y158 (= Y160)

1ipeA Tropinone reductase-ii complexed with NADPH (see paper)
32% identity, 96% coverage: 6:251/255 of query aligns to 4:252/259 of 1ipeA

query
sites
1ipeA

F

W

D

N

M

L

T

E

G

G

K

C

V

T

A

A

V

L

I

V

T

T

G
|
G

S

G

T
x
S

K
x
R

G
|
G

I
|
I

G

G

R

Y

A

G

I

I

A

V

G

E

E

E

M

L

A

A

V

S

C

L

G

G

A

A

K

S

V

V

V

Y

I

T

S

C

S
|
S

R
|
R

K

N

A

Q

E

K

A

E

C

L

E

N

Q

D

M

C

A

L

E

T

E

Q

L

W

K

R

A

S

Q

K

G

G

F

F

E

K

A

V

M

E

A

A

I

S

P

V

C
|
C

H
x
D

V
x
L

G

S

R

S

K

R

E

S

D

E

L

R

Q

Q

N

E

L

L

V

M

K

N

K

T

T

V

-

A

N

N

E

H

A

F

W

H

G

G

S

K

I

L

D

N

V

I

L

L

V

V

C

N

N
|
N

A

A

A

G

T

I

N

-

P

V

V

I

Y

Y

G

K

P

E

T

A

A

K

E

D

M

Y

T

T

D

V

D

E

A

D

W

Y

D

S

K

L

I

I

M

M

D

S

T
x
I

N

N

V

F

K

E

S

A

T

A

F

Y

W

H

L

L

T

S

N

V

M

L

V

A

L

H

P

P

Q

F

M

L

A

K

E

A

K

S

G

E

E

R

G

G

A

N

V

V

L

F

L

I

S

S

S
|
S

I

V

A

S

G

G

L

A

R

L

G

A

N

V

T

P

V

Y

I

E

G

A

T

V

Y
|
Y

G

G

V

A

S

T

K
|
K

A

G

A

A

E

M

A

D

A

Q

L

L

A

T

R

R

N

C

L

L

A

A

V

F

E

E

W

W

G

A

P

K

K

D

G

N

I

I

R

R

I

V

N

N

S

G

I

V

A

G

P
|
P

G

G

L

V

I
|
I

K

A

T
|
T

D
x
S

F
x
L

A
x
V

R

E

A

M

L

T

W

I

E

Q

D

D

P

P

E

E

R

Q

A

K

K

E

Q

N

A

L

E

N

-

K

-

L

-

I

D

D

R

R

T

C

P

A

L

L

R

R

I

M

G

G

D

E

P

P

V

K

D

E

I

L

A

A

G

A

L

M

A

V

V

A

F

F

L

L

S

C

T

F

K

P

A

A

S

A

A

S

Y

Y

I

V

T

T

G

G

Q

Q

V

I

I

I

V

Y

A

V

D

D

G

G

active site: G19 (= G21), S145 (= S147), Y158 (= Y160), K162 (= K164)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G15 (= G17), S17 (≠ T19), R18 (≠ K20), G19 (= G21), I20 (= I22), S39 (= S41), R40 (= R42), C64 (= C66), D65 (≠ H67), L66 (≠ V68), N93 (= N94), I116 (≠ T118), S145 (= S147), Y158 (= Y160), K162 (= K164), P188 (= P190), I191 (= I193), T193 (= T195), S194 (≠ D196), L195 (≠ F197), V196 (≠ A198)

Query Sequence

>GFF3984 FitnessBrowser__Marino:GFF3984
MNNPLFDMTGKVAVITGSTKGIGRAIAGEMAVCGAKVVISSRKAEACEQMAEELKAQGFE
AMAIPCHVGRKEDLQNLVKKTNEAWGSIDVLVCNAATNPVYGPTAEMTDDAWDKIMDTNV
KSTFWLTNMVLPQMAEKGEGAVVLLSSIAGLRGNTVIGTYGVSKAAEAALARNLAVEWGP
KGIRINSIAPGLIKTDFARALWEDPERAKQAEDRTPLRRIGDPVDIAGLAVFLSTKASAY
ITGQVIVADGGETIC

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory