SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF4001 FitnessBrowser__Marino:GFF4001 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

6iunB Crystal structure of enoyl-coa hydratase (ech) from ralstonia eutropha h16 in complex with NAD
52% identity, 98% coverage: 12:699/702 of query aligns to 4:690/692 of 6iunB

query
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6iunB

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active site: A60 (= A68), F65 (= F73), E73 (= E79), H77 (≠ P83), G101 (= G107), E104 (= E110), E124 (= E130), G132 (= G138), K248 (= K258), S407 (= S417), H428 (= H438), E440 (= E450), N478 (= N488)
binding nicotinamide-adenine-dinucleotide: G300 (= G310), T301 (= T311), M302 (= M312), E321 (= E331), T322 (≠ V332), Y365 (= Y375), A377 (= A387), V378 (= V388), E380 (= E390), V384 (≠ I394), V388 (≠ I398), N405 (= N415), S407 (= S417)

Q08426 Peroxisomal bifunctional enzyme; PBE; PBFE; L-bifunctional protein; LBP; Multifunctional enzyme 1; MFE1; EC 4.2.1.17; EC 5.3.3.8; EC 1.1.1.35 from Homo sapiens (Human) (see 5 papers)
43% identity, 98% coverage: 12:696/702 of query aligns to 4:705/723 of Q08426

query
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Q08426

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T

T

Q

G

A

K

G

G

V

W

Y

Y

K

Q

Y

Y

E

D

E
x
K

G

P

-

L

S

G

R

R

K

I

P

H

I

K

P

P

D

D

P

P

E

W

V

L

E

S

Q
x
K

L

F

I

L

E

S

Q

R

F

Y

R

R

K

K

E
x
T

Q

H

G

H

I

I

T

E

P

P

R

R

E

T

I

I

T

S

N

Q

Q

D

E

E

I

I

L

L

E

E

R

R

C

C

V

L

Y

Y

V

S

M

L

I

I

N

N

E

E

G

A

A

F

K

R

I

I

L

L

E

G

E

E

G

G

I

I

A

A

D

A

R

S

P

P

L

E

D

H

I

I

D

D

I

V

V

V

W

Y

I

L

Y

H

G

G

Y

Y

G

G

F

W

P

P

A

R

Y

H

R

K

G

G

G

G

P

P

M

M

F

F

W

Y

A

A

D

S

Q

T

E

V

G

G

L

L

D

P

T

T

I

V

L

L

S

E

A

K

V

L

K

Q

K

K

-

Y

Y

Y

Q

R

D

Q

T

N

V

P

G

D

G

I

E

P

Q

Q

W

L

E

E

P

P

A

S

A

D

L

Y

L

L

E

K

K

K

L

L

V

A

A

S

E

Q

G

G

V40 (≠ Q47) to G: in dbSNP:rs1062551
I41 (≠ K48) to R: in dbSNP:rs1062552
T75 (= T81) to I: in dbSNP:rs1062553
K165 (≠ A171) modified: N6-acetyllysine; alternate; mutation to Q: Greatly reduced acetylation and insensitive to treatment with TSA and NAM; when associated with Q-171; Q-346 and Q-584.
K171 (≠ A177) modified: N6-acetyllysine; mutation to Q: Greatly reduced acetylation and insensitive to treatment with TSA and NAM; when associated with Q-165; Q-346 and Q-584.
A274 (≠ G282) to T: in dbSNP:rs2302819
A325 (≠ I329) to G: in dbSNP:rs1062555
K346 (≠ N350) modified: N6-acetyllysine; mutation to Q: Greatly reduced acetylation and insensitive to treatment with TSA and NAM; when associated with Q-165; Q-171 and Q-584.
K584 (≠ E577) modified: N6-acetyllysine; alternate; mutation to Q: Greatly reduced acetylation and insensitive to treatment with TSA and NAM; when associated with Q-165; Q-171 and Q-346.
K598 (≠ Q590) to T: in dbSNP:rs1042437
T606 (≠ E598) to P: in dbSNP:rs1042438

Sites not aligning to the query:

3 E → K: in FRTS3; the mutant is mistargeted to mitochondria; results in impaired mitochondrial oxidative phosphorylation and defects in the transport of fluids across the epithelium of renal proximal tubular cells; dbSNP:rs398124646

6zibAAA structure of rat peroxisomal multifunctional enzyme type-1 (rpmfe1) complexed with acetoacetyl-coa and nadh' (see paper)
42% identity, 99% coverage: 2:696/702 of query aligns to 2:707/723 of 6zibAAA

query
sites
6zibAAA

R

R

G

G

E

S

I

H

M

M

S

A

E

E

V

Y

V

L

S

R

Y

L

N

P

R

H

E

-

G

-

N

S

I

L

G

A

V

M

I

I

T

R

V

L

N

C

Y

N

P

P

P
|
P

V
|
V

N

N

A

A

L

V

G

S

Q

P

A

T

V

V

R

I

S

R

G

E

L

V

L

R

A

N

A

G

L

L

E

Q

Q

K

G

A

Q

G

K

S

D

D

T

H

E

T

A

V

R

K

A

A

L

I

L

V

L

I

V

C

C

G

E

A

G

N

R

G

T

N

F

F

I

C

A
|
A

G
|
G

A
|
A

D
|
D

I
|
I

R

H

E

G

F
|
F

G

S

K

A

P

F

M

T

Q

P

E

G

P

L

T

A

L

L

P
x
G

T

S

L

L

V

V

N

D

T

E

F

I

E

Q

N

R

S

Y

D

Q

K

K

P

P

L

V

V

L

A

A

A

A

I

I

H

Q

G

G

T

V

A

A

L

L

G
|
G

G
|
G

G

G

L

L

E
|
E

T

L

A

A

L

L

S

G

C

C

H

H

Y

Y

R

R

V

I

A

A

I

N

S

A

S

K

A

A

K

R

V

V

G

G

L

L

P
|
P

E
|
E

V

V

K

T

L

L

G

G

L

I

L

L

P

P

G
|
G

A

A

G

R

G

G

T

T

Q

Q

R

L

L

L

P

P

R

R

L

V

T

V

G

G

A

V

R

P

K

V

A

A

L

L

E

D

M

L

I

I

T

T

T

S

G

G

E

K

F
x
Y

V

L

G

S

A

A

K

D

D

E

A

A

L

L

A

R

L

L

G

G

I

I

L

L

D

D

A

A

V

V

E

V

E

K

G

S

D

D

D

P

I

V

R

E

A

E

V

-

G

A

M

I

A

K

Y

F

A

A

Q

Q

K

K

V

I

V

I

D

D

E

-

G

-

K

K

P

P

V

I

R

E

R

P

V

R

R

R

D

I

I

F

T

N

D

K

K

P

I

V

E

P

A

S

D

L

K

P

G

N

S

M

D

D

-

S

V

V

F

F

D

A

Q

E

F

A

R

I

D

A

E

K

L

V

K

R

K

K

R

Q

A

Y

R

P

G

G

L

V

F

L

S

A

P

P

F

E

K

T

C

C

V

V

D

R

A

S

V

I

E

Q

A

A

A

S

F

V

N

K

L

H

P

P

F

Y

D

E

E

V

G

G

M

I

K
|
K

R

E

E

E

R

E

E

K

L

L

F

F

M

M

E

Y

C

L

M

R

E

A

S

S

P

G

Q

Q

R

A

A

K

G

A

L

L

I

Q

H

Y

S

A

F

F

F

F

G

A

E

E

R

K

E

S

V

A

S

N

K

K

V

W

K

S

G

T

L

P

S

S

-

G

-

A

-

S

-

W

K

K

D

T

T

A

P

S

V

A

R

Q

D

P

V

V

K

S

S

S

V

V

G

G

I

V

I

L

G

G

A

L

G
|
G

T
|
T

M
|
M

G

G

G

R

G

G

I

I

A

A

M

I

N

S

F

F

V

A

N

R

V

V

G

G

I

I

P

S

V

V

T

V

I

A

V

V

E
|
E

V
x
S

K

D

Q

P

E

K

A
x
Q

L

L

D

D

K

A

G

A

L

K

A

K

I

I

I

I

R

T

R

F

N

T

Y

L

E

E

N

K

S

E

A

A

K

S

K

R

G

A

K

-

L

-

T

-

Q

-

E

H

Q

Q

V

N

E

G

Q

Q

R

A

M

K

A

P

L

K

I

L

T

R

P

F

S

S

L

S

T

S

Y

T

D

K

D

E

F

L

R

S

D

T

V

V

D

D

L

L

V

V

I

V

E

E

A
|
A

V
|
V

F
|
F

E
|
E

N

D

M

M

A

N

I

L

K

K

K

K

E

K

I

V

F

F

A

A

K

E

L

L

D

S

E

A

V

L

C

C

K

K

P

P

G

G

A

A

I

F

L

L

A

C

S

T

N
|
N

T

T

S
|
S

T

A

L

L

D

N

I

V

D

D

E

D

I

I

A

A

S

S

A

S

T

T

K

D

R

R

P

P

E

Q

D

L

V

V

V

I

G

G

M

T

H
|
H

F

F

F

F

S

S

P

P

A

A

N

H

V

V

M

M

K

R

L

L

L

L

E
|
E

N

V

V

I

R

P

G

S

S

R

K

Y

T

S

S

S

D

P

E

T

V

T

K

I

A

A

T

T

V

V

M

M

A

S

V

L

A

S

K

K

K

K

I

I

K

G

K

K

V

I

G

G

V

V

M

V

V

V

G

G

N

N

C

C

Y

Y

G

G

F

F

V

V

G

G

N
|
N

R

R

M

M

L

L

H

A

K

P

R

Y

G

Y

T

N

E

Q

A

G

M

F

S

F

L

L

V

L

D

E

E

E

G

G

A

S

T

K

P

P

Q

E

Q

D

V

V

D

D

K

G

V

V

L

L

T

E

D

E

L

F

G

G

F

F

P

K

M

M

G

G

Q

P

F

F

A

R

M

V

S

S

D

D

L

L

A

A

G

G

I

L

D

D

V

V

G

G

Y

W

R

K

I

I

R

R

E

K

E

G

R

Q

R

G

K

L

A

T

G

G

E

P

D

S

I

L

P

P

-

P

-

G

-

T

-

P

-

V

-

R

-

K

-

R

-

G

-

N

-

S

-

R

-

Y

A

S

S

P

W

L

M

G

D

D

K

M

L

L

A

C

E

E

Q

A

G

G

R

R

L

F

G

G

Q

Q

K

K

T

T

Q

G

A

K

G

G

V

W

Y

Y

K

Q

Y

Y

E

D

E

K

G

P

-

L

S

G

R

R

K

I

P

H

I

K

P

P

D

D

P

P

E

W

V

L

E

S

Q

T

L

F

I

L

E

S

Q

Q

F

Y

R

R

K

E

E

V

Q

H

G

H

I

I

T

E

P

Q

R

R

E

T

I

I

T

S

N

K

Q

E

E

E

I

I

L

L

E

E

R

R

C

C

V

L

Y

Y

V

S

M

L

I

I

N

N

E

E

G

A

A

F

K

R

I

I

L

L

E

E

E

E

G

G

I

M

A

A

D

A

R

R

P

P

L

E

D

H

I

I

D

D

I

V

V

I

W

Y

I

L

Y

H

G

G

Y

Y

G

G

F

W

P

P

A

R

Y

H

R

K

G

G

G

G

P

P

M

M

F

F

W

Y

A

A

D

A

Q

S

E

V

G

G

L

L

D

P

T

T

I

V

L

L

S

E

A

K

V

L

K

Q

K

K

-

Y

Y

Y

Q

R

D

Q

T

N

V

P

G

D

G

I

E

P

Q

Q

W

L

E

E

P

P

A

S

A

D

L

Y

L

L

E

R

K

R

L

L

V

V

A

A

E

Q

G

G

active site: A66 (= A68), F71 (= F73), G81 (≠ P83), G105 (= G107), E108 (= E110), P127 (= P129), E128 (= E130), G136 (= G138), K254 (= K258), S413 (= S417), H434 (= H438), E446 (= E450), N484 (= N488)
binding acetoacetyl-coenzyme a: P25 (= P27), V26 (= V28), A64 (= A66), G65 (= G67), A66 (= A68), D67 (= D69), I68 (= I70), G104 (= G106), G105 (= G107), E128 (= E130), Y161 (≠ F163)
binding 1,4-dihydronicotinamide adenine dinucleotide: G310 (= G310), T311 (= T311), M312 (= M312), E331 (= E331), S332 (≠ V332), Q336 (≠ A336), A383 (= A387), V384 (= V388), F385 (= F389), E386 (= E390), N411 (= N415), H434 (= H438)

6z5oAAA Peroxisomal bifunctional enzyme (see paper)
42% identity, 99% coverage: 2:696/702 of query aligns to 3:703/716 of 6z5oAAA

query
sites
6z5oAAA

R

R

G

G

E

S

I

H

M

M

S

A

E

E

V

Y

V

L

S

R

Y

L

N

P

R

H

E

-

G

-

N

S

I

L

G

A

V

M

I

I

T

R

V

L

N

C

Y

N

P

P

P
|
P

V
|
V

N

N

A

A

L

V

G

S

Q

P

A

T

V

V

R

I

S

R

G

E

L

V

L

R

A

N

A

G

L

L

E

Q

Q

K

G

A

Q

G

K

S

D

D

T

H

E

T

A

V

R

K

A

A

L

I

L

V

L

I

V

C

C

G

E

A

G

N

R

G

T

N

F

F

I

C

A
|
A

G

G

A
|
A

D
|
D

I
|
I

R

H

E

G

F
|
F

G

S

K

A

P

F

M

T

Q

P

E

G

P

L

T

A

L

L

P
x
G

T

S

L

L

V

V

N

D

T

E

F

I

E

Q

N

R

S

Y

D

Q

K

K

P

P

L

V

V

L

A

A

A

A

I

I

H

Q

G

G

T

V

A

A

L

L

G

G

G
|
G

G

G

L

L

E
|
E

T

L

A

A

L

L

S

G

C

C

H

H

Y

Y

R

R

V

I

A

A

I

N

S

A

S

K

A

A

K

R

V

V

G

G

L

L

P
|
P

E
|
E

V

V

K

T

L

L

G

G

L

I

L

L

P
|
P

G
|
G

A

A

G

R

G

G

T

T

Q

Q

R

L

L

L

P

P

R

R

L

V

T

V

G

G

A

V

R

P

K

V

A

A

L

L

E

D

M

L

I

I

T

T

T

S

G

G

E

K

F
x
Y

V

L

G

S

A

A

K

D

D

E

A

A

L

L

A

R

L

L

G

G

I

I

L

L

D

D

A

A

V

V

E

V

E

K

G

S

D

D

D

P

I

V

R

E

A

E

V

-

G

A

M

I

A

K

Y

F

A

A

Q

Q

K

K

V

I

V

I

D

D

E

-

G

-

K

K

P

P

V

I

R

E

R

P

V

R

R

R

D

I

I

F

T

N

D

K

K

P

I

V

E

P

A

S

D

L

K

P

G

N

S

M

D

D

-

S

V

V

F

F

D

A

Q

E

F

A

R

I

D

A

E

K

L

V

K

R

K

K

R

Q

A

Y

R

P

G

G

L

V

F

L

S

A

P

P

F

E

K

T

C

C

V

V

D

R

A

S

V

I

E

Q

A

A

A

S

F

V

N

K

L

H

P

P

F

Y

D

E

E

V

G

G

M

I

K
|
K

R

E

E

E

R

E

E

K

L

L

F
|
F

M

M

E

Y

C

L

M

R

E

A

S

S

P

G

Q

Q

R

A

A

K

G

A

L

L

I

Q

H

Y

S

A

F
|
F

F

F

G

A

E

E

R
x
K

E

S

V

A

S

N

K

K

V

W

K

S

G

T

L

P

S

S

-

G

-

A

-

S

-

W

K

K

D

T

T

A

P

S

V

A

R

Q

D

P

V

V

K

S

S

S

V

V

G

G

I

V

I

L

G
|
G

A

L

G
|
G

T
|
T

M
|
M

G

G

G

R

G

G

I

I

A

A

M

I

N

S

F

F

V

A

N

R

V

V

G

G

I

I

P

S

V

V

T

V

I

A

V

V

E
|
E

V
x
S

K

D

Q

P

E

K

A
x
Q

L

L

D

D

K

A

G

A

L

K

A

K

I

I

I

I

R

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D

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I

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A

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E
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E

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K

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K

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F

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C

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K

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P

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G

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A

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active site: A67 (= A68), F72 (= F73), G82 (≠ P83), G106 (= G107), E109 (= E110), P128 (= P129), E129 (= E130), G137 (= G138), K255 (= K258), S409 (= S417), H430 (= H438), E442 (= E450), N480 (= N488)
binding coenzyme a: P26 (= P27), V27 (= V28), A65 (= A66), D68 (= D69), I69 (= I70), P128 (= P129), Y162 (≠ F163), F277 (= F280), K281 (≠ R284)
binding nicotinamide-adenine-dinucleotide: G309 (= G308), G311 (= G310), T312 (= T311), M313 (= M312), E332 (= E331), S333 (≠ V332), Q337 (≠ A336), A379 (= A387), V380 (= V388), F381 (= F389), E382 (= E390), K387 (= K395), N407 (= N415), S409 (= S417), H430 (= H438)
binding nicotinamide: A67 (= A68), E109 (= E110), E129 (= E130), P136 (= P137), F261 (= F264)

5omoA Crystal structure of rat peroxisomal multifunctional enzyme type-1 (rpmfe1) complexed with with 3s-hydroxy-decanoyl-coa and 3-keto- decanoyl-coa
42% identity, 99% coverage: 2:696/702 of query aligns to 2:709/725 of 5omoA

query
sites
5omoA

R

R

G

G

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N

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Y
|
Y

G

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F

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A

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G

G

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P

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M

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D

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active site: A66 (= A68), F71 (= F73), G81 (≠ P83), G105 (= G107), E108 (= E110), P127 (= P129), E128 (= E130), P135 (= P137), G136 (= G138), K254 (= K258), S415 (= S417), H436 (= H438), E448 (= E450), N486 (= N488)
binding (s)-3-hydroxydecanoyl-coa: P25 (= P27), V26 (= V28), A28 (= A30), P31 (≠ Q33), A64 (= A66), A66 (= A68), D67 (= D69), I68 (= I70), L103 (= L105), G105 (= G107), E108 (= E110), P127 (= P129), E128 (= E130), Y161 (≠ F163), F260 (= F264), K280 (≠ R284)
binding 3-keto-decanoyl-coa: S415 (= S417), N486 (= N488), K519 (≠ P521), M520 (= M522), V525 (≠ M527), Y658 (= Y646)

5mgbA Crystal structure of rat peroxisomal multifunctional enzyme type-1 (rpmfe1) complexed with acetoacetyl-coa and NAD (see paper)
42% identity, 99% coverage: 2:696/702 of query aligns to 2:709/725 of 5mgbA

query
sites
5mgbA

R

R

G

G

E

S

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A

E

E

V

Y

V

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active site: A66 (= A68), F71 (= F73), G81 (≠ P83), G105 (= G107), E108 (= E110), P127 (= P129), E128 (= E130), P135 (= P137), G136 (= G138), K254 (= K258), S415 (= S417), H436 (= H438), E448 (= E450), N486 (= N488)
binding acetoacetyl-coenzyme a: P25 (= P27), V26 (= V28), A64 (= A66), G65 (= G67), A66 (= A68), D67 (= D69), I68 (= I70), G105 (= G107), E128 (= E130), Y161 (≠ F163)
binding nicotinamide-adenine-dinucleotide: L307 (≠ I307), G308 (= G308), G310 (= G310), T311 (= T311), M312 (= M312), E331 (= E331), S332 (≠ V332), Q336 (≠ A336), V386 (= V388), F387 (= F389), E388 (= E390), N413 (= N415), S415 (= S417), H436 (= H438)

3zwcA Crystal structure of rat peroxisomal multifunctional enzyme type 1 (rpmfe1) complexed with 3s-hydroxy-decanoyl-coa (see paper)
42% identity, 99% coverage: 2:696/702 of query aligns to 2:709/725 of 3zwcA

query
sites
3zwcA

R

R

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D

Q

T

N

V

P

G

D

G

I

E

P

Q

Q

W

L

E

E

P

P

A

S

A

D

L

Y

L

L

E

R

K

R

L

L

V

V

A

A

E

Q

G

G

active site: A66 (= A68), F71 (= F73), G81 (≠ P83), G105 (= G107), E108 (= E110), P127 (= P129), E128 (= E130), P135 (= P137), G136 (= G138), K254 (= K258), S415 (= S417), H436 (= H438), E448 (= E450), N486 (= N488)
binding (s)-3-hydroxydecanoyl-coa: V26 (= V28), A64 (= A66), G65 (= G67), A66 (= A68), D67 (= D69), I68 (= I70), G77 (≠ E79), L78 (≠ P80), L80 (= L82), V101 (≠ T103), G104 (= G106), G105 (= G107), E108 (= E110), E128 (= E130), F260 (= F264)
binding nicotinamide-adenine-dinucleotide: G308 (= G308), G310 (= G310), T311 (= T311), M312 (= M312), E331 (= E331), Q336 (≠ A336), A385 (= A387), V386 (= V388), F387 (= F389), E388 (= E390), K393 (= K395), N413 (= N415), S415 (= S417), H436 (= H438)

2x58A The crystal structure of mfe1 liganded with coa (see paper)
42% identity, 99% coverage: 2:696/702 of query aligns to 2:709/725 of 2x58A

query
sites
2x58A

R

R

G

G

E

S

I

H

M

M

S

A

E

E

V

Y

V

L

S

R

Y

L

N

P

R

H

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-

G

-

N

S

I

L

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I

I

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C

Y

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P

P

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|
V

N

N

A
|
A

L

V

G

S

Q

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A

T

V

V

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I

S

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L

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L

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A

A

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L

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C

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N

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F

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A
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A

G

G

A
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A

D
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D

I
|
I

R

H

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F
|
F

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G

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|
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|
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|
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|
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|
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F

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|
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|
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|
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|
N

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L

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L

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I

I

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I

L

L

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C

C

V

L

Y

Y

V

S

M

L

I

I

N

N

E

E

G

A

A

F

K

R

I

I

L

L

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E

E

E

G

G

I

M

A

A

D

A

R

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P

P

L

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D

H

I

I

D

D

I

V

V

I

W

Y

I

L

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G

G

Y

Y

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G

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W

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A

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Y

H

R

K

G

G

G

G

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P

M

M

F

F

W

Y

A

A

D

A

Q

S

E

V

G

G

L

L

D

P

T

T

I

V

L

L

S

E

A

K

V

L

K

Q

K

K

-

Y

Y

Y

Q

R

D

Q

T

N

V

P

G

D

G

I

E

P

Q

Q

W

L

E

E

P

P

A

S

A

D

L

Y

L

L

E

R

K

R

L

L

V

V

A

A

E

Q

G

G

active site: A66 (= A68), F71 (= F73), G81 (≠ P83), G105 (= G107), E108 (= E110), P127 (= P129), E128 (= E130), P135 (= P137), G136 (= G138), K254 (= K258), S415 (= S417), H436 (= H438), E448 (= E450), N486 (= N488)
binding adenosine-5'-diphosphate: G310 (= G310), T311 (= T311), M312 (= M312), E331 (= E331), S332 (≠ V332), Q336 (≠ A336), V386 (= V388), L392 (≠ I394)
binding coenzyme a: V26 (= V28), A28 (= A30), A64 (= A66), A66 (= A68), D67 (= D69), I68 (= I70), E128 (= E130)

3zwaA Crystal structure of rat peroxisomal multifunctional enzyme type 1 (rpmfe1) complexed with 3s-hydroxy-hexanoyl-coa (see paper)
42% identity, 99% coverage: 2:696/702 of query aligns to 3:710/727 of 3zwaA

query
sites
3zwaA

R

R

G

G

E

S

I

H

M

M

S

A

E

E

V

Y

V

L

S

R

Y

L

N

P

R

H

E

-

G

-

N

S

I

L

G

A

V

M

I

I

T

R

V

L

N

C

Y

N

P

P

P

P

V
|
V

N

N

A

A

L

V

G

S

Q

P

A

T

V

V

R

I

S

R

G

E

L

V

L

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A

N

A

G

L

L

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Q

Q

K

G

A

Q

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D

D

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H

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V

R

K

A

A

L

I

L

V

L

I

V

C

C

G

E

A

G

N

R

G

T

N

F

F

I

C

A
|
A

G
|
G

A
|
A

D
|
D

I
|
I

R

H

E

G

F
|
F

G

S

K

A

P

F

M

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L

T

A

L

L

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x
G

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L

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D

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V

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A

A

A

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I

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Q

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G

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A

A

L
|
L

G

G

G
|
G

G

G

L

L

E
|
E

T

L

A

A

L

L

S

G

C

C

H

H

Y

Y

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R

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A

A

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A

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R

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G

L

L

P
|
P

E
|
E

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V

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|
L

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G

L

I

L

L

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P

G
|
G

A

A

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L

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K

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L

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N

N

T

T

S
|
S

T

A

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L

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D

D

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D

I

I

A

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|
H

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A

A

N

H

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M

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|
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I

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K

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M

V

V

V

G

G

N

N

C

C

Y

Y

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G

F

F

V

V

G

G

N
|
N

R

R

M

M

L

L

H

A

K

P

R

Y

G

Y

T

N

E

Q

A

G

M

F

S

F

L

L

V

L

D

E

E

E

G

G

A

S

T

K

P

P

Q

E

Q

D

V

V

D

D

K

G

V

V

L

L

T

E

D

E

L

F

G

G

F

F

P

K

M

M

G

G

Q

P

F

F

A

R

M

V

S

S

D

D

L

L

A

A

G

G

I

L

D

D

V

V

G

G

Y

W

R

K

I

I

R

R

E

K

E

G

R

Q

R

G

K

L

A

T

G

G

E

P

D

S

I

L

P

P

-

P

-

G

-

T

-

P

-

V

-

R

-

K

-

R

-

G

-

N

-

S

-

R

-

Y

A

S

S

P

W

L

M

G

D

D

K

M

L

L

A

C

E

E

Q

A

G

G

R

R

L

F

G

G

Q

Q

K

K

T

T

Q

G

A

K

G

G

V

W

Y

Y

K

Q

Y

Y

E

D

E

K

G

P

-

L

S

G

R

R

K

I

P

H

I

K

P

P

D

D

P

P

E

W

V

L

E

S

Q

T

L

F

I

L

E

S

Q

Q

F

Y

R

R

K

E

E

V

Q

H

G

H

I

I

T

E

P

Q

R

R

E

T

I

I

T

S

N

K

Q

E

E

E

I

I

L

L

E

E

R

R

C

C

V

L

Y

Y

V

S

M

L

I

I

N

N

E

E

G

A

A

F

K

R

I

I

L

L

E

E

E

E

G

G

I

M

A

A

D

A

R

R

P

P

L

E

D

H

I

I

D

D

I

V

V

I

W

Y

I

L

Y

H

G

G

Y

Y

G

G

F

W

P

P

A

R

Y

H

R

K

G

G

G

G

P

P

M

M

F

F

W

Y

A

A

D

A

Q

S

E

V

G

G

L

L

D

P

T

T

I

V

L

L

S

E

A

K

V

L

K

Q

K

K

-

Y

Y

Y

Q

R

D

Q

T

N

V

P

G

D

G

I

E

P

Q

Q

W

L

E

E

P

P

A

S

A

D

L

Y

L

L

E

R

K

R

L

L

V

V

A

A

E

Q

G

G

active site: A67 (= A68), F72 (= F73), G82 (≠ P83), G106 (= G107), E109 (= E110), P128 (= P129), E129 (= E130), P136 (= P137), G137 (= G138), K255 (= K258), S416 (= S417), H437 (= H438), E449 (= E450), N487 (= N488)
binding (S)-3-Hydroxyhexanoyl-CoA: V27 (= V28), A65 (= A66), G66 (= G67), A67 (= A68), D68 (= D69), I69 (= I70), L104 (= L105), E109 (= E110), R124 (≠ K125), E129 (= E130), L132 (= L133), G137 (= G138), Y162 (≠ F163)

6zicAAA structure of rat peroxisomal multifunctional enzyme type-1 (rpmfe1) complexed with 3s-hydroxybutanoyl-coa and nadh' (see paper)
42% identity, 99% coverage: 2:696/702 of query aligns to 2:707/723 of 6zicAAA

query
sites
6zicAAA

R

R

G

G

E

S

I

H

M

M

S

A

E

E

V

Y

V

L

S

R

Y

L

N

P

R

H

E

-

G

-

N

S

I

L

G

A

V

M

I

I

T

R

V

L

N

C

Y

N

P

P

P
|
P

V
|
V

N

N

A
|
A

L

V

G

S

Q

P

A

T

V

V

R

I

S

R

G

E

L

V

L

R

A

N

A

G

L

L

E

Q

Q

K

G

A

Q

G

K

S

D

D

T

H

E

T

A

V

R

K

A

A

L

I

L

V

L

I

V

C

C

G

E

A

G

N

R

G

T

N

F

F

I

C

A

A

G

G

A
|
A

D
|
D

I
|
I

R

H

E

G

F
|
F

G

S

K

A

P

F

M

T

Q

P

E

G

P

L

T

A

L

L

P
x
G

T

S

L

L

V

V

N

D

T

E

F

I

E

Q

N

R

S

Y

D

Q

K

K

P

P

L

V

V

L

A

A

A

A

I

I

H

Q

G

G

T

V

A

A

L

L

G
|
G

G
|
G

G

G

L

L

E
|
E

T

L

A

A

L

L

S

G

C

C

H

H

Y

Y

R

R

V

I

A

A

I

N

S

A

S

K

A

A

K

R

V

V

G

G

L

L

P
|
P

E
|
E

V

V

K

T

L

L

G

G

L

I

L

L

P

P

G
|
G

A

A

G

R

G

G

T

T

Q

Q

R

L

L

L

P

P

R

R

L

V

T

V

G

G

A

V

R

P

K

V

A

A

L

L

E

D

M

L

I

I

T

T

T

S

G

G

E

K

F
x
Y

V

L

G

S

A

A

K

D

D

E

A

A

L

L

A

R

L

L

G

G

I

I

L

L

D

D

A

A

V

V

E

V

E

K

G

S

D

D

D

P

I

V

R

E

A

E

V

-

G

A

M

I

A

K

Y

F

A

A

Q

Q

K

K

V

I

V

I

D

D

E

-

G

-

K

K

P

P

V

I

R

E

R

P

V

R

R

R

D

I

I

F

T

N

D

K

K

P

I

V

E

P

A

S

D

L

K

P

G

N

S

M

D

D

-

S

V

V

F

F

D

A

Q

E

F

A

R

I

D

A

E

K

L

V

K

R

K

K

R

Q

A

Y

R

P

G

G

L

V

F

L

S

A

P

P

F

E

K

T

C

C

V

V

D

R

A

S

V

I

E

Q

A

A

A

S

F

V

N

K

L

H

P

P

F

Y

D

E

E

V

G

G

M

I

K
|
K

R

E

E

E

R

E

E

K

L

L

F

F

M

M

E

Y

C

L

M

R

E

A

S

S

P

G

Q

Q

R

A

A

K

G

A

L

L

I

Q

H

Y

S

A

F

F

F

F

G

A

E

E

R

K

E

S

V

A

S

N

K

K

V

W

K

S

G

T

L

P

S

S

-

G

-

A

-

S

-

W

K

K

D

T

T

A

P

S

V

A

R

Q

D

P

V

V

K

S

S

S

V

V

G

G

I

V

I

L

G
|
G

A

L

G
|
G

T
|
T

M
|
M

G

G

G

R

G

G

I

I

A

A

M

I

N

S

F

F

V

A

N

R

V

V

G

G

I

I

P

S

V

V

T

V

I

A

V

V

E
|
E

V
x
S

K

D

Q

P

E

K

A
x
Q

L

L

D

D

K

A

G

A

L

K

A

K

I

I

I

I

R

T

R

F

N

T

Y

L

E

E

N

K

S

E

A

A

K

S

K

R

G

A

K

-

L

-

T

-

Q

-

E

H

Q

Q

V

N

E

G

Q

S

R

A

M

K

A

P

L

K

I

L

T

R

P

F

S

S

L

S

T

S

Y

T

D

K

D

E

F

L

R

S

D

T

V

V

D

D

L

L

V

V

I

V

E

E

A
|
A

V
|
V

F
|
F

E
|
E

N

D

M

M

A

N

I
x
L

K
|
K

K

K

E

K

I

V

F

F

A

A

K

E

L

L

D

S

E

A

V

L

C

C

K

K

P

P

G

G

A

A

I

F

L

L

A

C

S

T

N
|
N

T

T

S
|
S

T

A

L

L

D

N

I

V

D

D

E

D

I

I

A

A

S

S

A

S

T

T

K

D

R

R

P

P

E

Q

D

L

V

V

V

I

G

G

M

T

H
|
H

F

F

F

F

S

S

P

P

A

A

N

H

V

V

M

M

K

R

L

L

L

L

E
|
E

N

V

V

I

R

P

G

S

S

R

K

Y

T

S

S

S

D

P

E

T

V

T

K

I

A

A

T

T

V

V

M

M

A

S

V

L

A

S

K

K

K

K

I

I

K

G

K

K

V

I

G

G

V

V

M

V

V

V

G

G

N

N

C

C

Y

Y

G

G

F

F

V

V

G

G

N
|
N

R

R

M

M

L

L

H

A

K

P

R

Y

G

Y

T

N

E

Q

A

G

M

F

S

F

L

L

V

L

D

E

E

E

G

G

A

S

T

K

P

P

Q

E

Q

D

V

V

D

D

K

G

V

V

L

L

T

E

D

E

L

F

G

G

F

F

P

K

M

M

G

G

Q

P

F

F

A

R

M

V

S

S

D

D

L

L

A

A

G

G

I

L

D

D

V

V

G

G

Y

W

R

K

I

I

R

R

E

K

E

G

R

Q

R

G

K

L

A

T

G

G

E

P

D

S

I

L

P

P

-

P

-

G

-

T

-

P

-

V

-

R

-

K

-

R

-

G

-

N

-

S

-

R

-

Y

A

S

S

P

W

L

M

G

D

D

K

M

L

L

A

C

E

E

Q

A

G

G

R

R

L

F

G

G

Q

Q

K

K

T

T

Q

G

A

K

G

G

V

W

Y

Y

K

Q

Y

Y

E

D

E

K

G

P

-

L

S

G

R

R

K

I

P

H

I

K

P

P

D

D

P

P

E

W

V

L

E

S

Q

T

L

F

I

L

E

S

Q

Q

F

Y

R

R

K

E

E

V

Q

H

G

H

I

I

T

E

P

Q

R

R

E

T

I

I

T

S

N

K

Q

E

E

E

I

I

L

L

E

E

R

R

C

C

V

L

Y

Y

V

S

M

L

I

I

N

N

E

E

G

A

A

F

K

R

I

I

L

L

E

E

E

E

G

G

I

M

A

A

D

A

R

R

P

P

L

E

D

H

I

I

D

D

I

V

V

I

W

Y

I

L

Y

H

G

G

Y

Y

G

G

F

W

P

P

A

R

Y

H

R

K

G

G

G

G

P

P

M

M

F

F

W

Y

A

A

D

A

Q

S

E

V

G

G

L

L

D

P

T

T

I

V

L

L

S

E

A

K

V

L

K

Q

K

K

-

Y

Y

Y

Q

R

D

Q

T

N

V

P

G

D

G

I

E

P

Q

Q

W

L

E

E

P

P

A

S

A

D

L

Y

L

L

E

R

K

R

L

L

V

V

A

A

E

Q

G

G

active site: A66 (= A68), F71 (= F73), G81 (≠ P83), G105 (= G107), E108 (= E110), P127 (= P129), E128 (= E130), G136 (= G138), K254 (= K258), S413 (= S417), H434 (= H438), E446 (= E450), N484 (= N488)
binding 3-hydroxybutanoyl-coenzyme a: P25 (= P27), V26 (= V28), A28 (= A30), A66 (= A68), D67 (= D69), I68 (= I70), G104 (= G106), G105 (= G107), E108 (= E110), E128 (= E130), Y161 (≠ F163)
binding 1,4-dihydronicotinamide adenine dinucleotide: G308 (= G308), G310 (= G310), T311 (= T311), M312 (= M312), E331 (= E331), S332 (≠ V332), Q336 (≠ A336), A383 (= A387), V384 (= V388), F385 (= F389), E386 (= E390), L390 (≠ I394), K391 (= K395), N411 (= N415), S413 (= S417), H434 (= H438)

3zw9A Crystal structure of rat peroxisomal multifunctional enzyme type 1 (rpmfe1) complexed with (2s,3s)-3-hydroxy-2-methylbutanoyl-coa (see paper)
42% identity, 98% coverage: 7:696/702 of query aligns to 2:707/723 of 3zw9A

query
sites
3zw9A

S

S

E

H

V

M

V

A

S

E

Y

Y

N

L

R

R

-

L

E

P

G

H

N

S

I

L

G

A

V

M

I

I

T

R

V

L

N

C

Y

N

P

P

P

P

V
|
V

N

N

A

A

L

V

G

S

Q

P

A

T

V

V

R

I

S

R

G

E

L

V

L

R

A

N

A

G

L

L

E

Q

Q

K

G

A

Q

G

K

S

D

D

T

H

E

T

A

V

R

K

A

A

L

I

L

V

L

I

V

C

C

G

E

A

G

N

R

G

T

N

F

F

I

C

A
|
A

G
|
G

A
|
A

D

D

I
|
I

R

H

E

G

F
|
F

G

S

K

A

P

F

M

T

Q

P

E

G

P

L

T

A

L

L

P
x
G

T

S

L

L

V

V

N

D

T

E

F

I

E

Q

N

R

S

Y

D

Q

K

K

P

P

L

V

V

L

A

A

A

A

I

I

H

Q

G

G

T

V

A

A

L

L

G
|
G

G
|
G

G

G

L

L

E
|
E

T

L

A

A

L

L

S

G

C

C

H

H

Y

Y

R

R

V

I

A

A

I

N

S

A

S

K

A

A

K

R

V

V

G

G

L

L

P
|
P

E
|
E

V

V

K

T

L

L

G

G

L

I

L

L

P
|
P

G
|
G

A

A

G

R

G

G

T

T

Q

Q

R

L

L

L

P

P

R

R

L

V

T

V

G

G

A

V

R

P

K

V

A

A

L

L

E

D

M

L

I

I

T

T

T

S

G

G

E

K

F
x
Y

V

L

G

S

A

A

K

D

D

E

A

A

L

L

A

R

L

L

G

G

I

I

L

L

D

D

A

A

V

V

E

V

E

K

G

S

D

D

D

P

I

V

R

E

A

E

V

-

G

A

M

I

A

K

Y

F

A

A

Q

Q

K

K

V

I

V

I

D

D

E

-

G

-

K

K

P

P

V

I

R

E

R

P

V

R

R

R

D

I

I

F

T

N

D

K

K

P

I

V

E

P

A

S

D

L

K

P

G

N

S

M

D

D

-

S

V

V

F

F

D

A

Q

E

F

A

R

I

D

A

E

K

L

V

K

R

K

K

R

Q

A

Y

R

P

G

G

L

V

F

L

S

A

P

P

F

E

K

T

C

C

V

V

D

R

A

S

V

I

E

Q

A

A

A

S

F

V

N

K

L

H

P

P

F

Y

D

E

E

V

G

G

M

I

K
|
K

R

E

E

E

R

E

E

K

L

L

F

F

M

M

E

Y

C

L

M

R

E

A

S

S

P

G

Q

Q

R

A

A

K

G

A

L

L

I

Q

H

Y

S

A

F

F

F

F

G

A

E

E

R

K

E

S

V

A

S

N

K

K

V

W

K

S

G

T

L

P

S

S

-

G

-

A

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-

W

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K

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S

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A

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V

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G

I

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x
L

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G

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G

T
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T

M
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M

G

G

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G

G

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I

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C

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P

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L

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T

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Y

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N

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A

A

F

K

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I

L

L

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G

I

M

A

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D

A

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P

L

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D

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I

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D

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V

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Y

I

L

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Y

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A

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H

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K

G

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P

M

M

F

F

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Y

A

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G

L

L

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T

T

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V

L

L

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L

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K

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Y

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V

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L

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K

R

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L

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V

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active site: A64 (= A68), F69 (= F73), G79 (≠ P83), G103 (= G107), E106 (= E110), P125 (= P129), E126 (= E130), P133 (= P137), G134 (= G138), K252 (= K258), S413 (= S417), H434 (= H438), E446 (= E450), N484 (= N488)
binding nicotinamide-adenine-dinucleotide: L305 (≠ I307), G306 (= G308), G308 (= G310), T309 (= T311), M310 (= M312), E329 (= E331), Q334 (≠ A336), A383 (= A387), V384 (= V388), F385 (= F389), E386 (= E390), N411 (= N415), S413 (= S417), H434 (= H438)
binding (2s,3s)-3-hydroxy-2-methylbutanoyl-coa: V24 (= V28), A62 (= A66), G63 (= G67), A64 (= A68), I66 (= I70), G102 (= G106), G103 (= G107), E106 (= E110), E126 (= E130), P133 (= P137), Y159 (≠ F163)

3zwbA Crystal structure of rat peroxisomal multifunctional enzyme type 1 (rpmfe1) complexed with 2trans-hexenoyl-coa (see paper)
41% identity, 99% coverage: 2:696/702 of query aligns to 2:709/725 of 3zwbA

query
sites
3zwbA

R

R

G

G

E

S

I

H

M

M

S

A

E

E

V

Y

V

L

S

R

Y

L

N

P

R

H

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-

G

-

N

S

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L

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A

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Y

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|
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A
|
A

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A
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I
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I

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F

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A

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x
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K

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P

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A

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C

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K

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I

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H

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|
N

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Q

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Y

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G

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P

H

I

K

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P

D

D

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P

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L

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T

L

F

I

L

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S

Q

Q

F

Y

R

R

K

E

E

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Q

H

G

H

I

I

T

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P

Q

R

R

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I

I

T

S

N

K

Q

E

E

E

I

I

L

L

E

E

R

R

C

C

V

L

Y

Y

V

S

M

L

I

I

N

N

E

E

G

A

A

F

K

R

I

I

L

L

E

E

E

E

G

G

I

M

A

A

D

A

R

R

P

P

L

E

D

H

I

I

D

D

I

V

V

I

W

Y

I

L

Y

H

G

G

Y

Y

G

G

F

W

P

P

A

R

Y

H

R

K

G

G

G

G

P

P

M

M

F

F

W

Y

A

A

D

A

Q

S

E

V

G

G

L

L

D

P

T

T

I

V

L

L

S

E

A

K

V

L

K

Q

K

K

-

Y

Y

Y

Q

R

D

Q

T

N

V

P

G

D

G

I

E

P

Q

Q

W

L

E

E

P

P

A

S

A

D

L

Y

L

L

E

R

K

R

L

L

V

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A

A

E

Q

G

G

active site: A66 (= A68), G81 (≠ P83), G105 (= G107), E108 (= E110), P127 (= P129), A128 (≠ E130), P135 (= P137), G136 (= G138), S415 (= S417), H436 (= H438), E448 (= E450), N486 (= N488)
binding (2E)-Hexenoyl-CoA: P25 (= P27), V26 (= V28), A28 (= A30), A64 (= A66), G65 (= G67), A66 (= A68), D67 (= D69), I68 (= I70), V101 (≠ T103), L103 (= L105), G105 (= G107), E108 (= E110), G136 (= G138), Y161 (≠ F163), K280 (≠ R284)

P40939 Trifunctional enzyme subunit alpha, mitochondrial; 78 kDa gastrin-binding protein; Monolysocardiolipin acyltransferase; TP-alpha; EC 2.3.1.-; EC 4.2.1.17; EC 1.1.1.211 from Homo sapiens (Human) (see 5 papers)
32% identity, 97% coverage: 10:689/702 of query aligns to 41:750/763 of P40939

query
sites
P40939

V

I

S

N

Y

Y

N

G

R

V

E

K

G

G

N

D

I

V

G

A

V

V

I

V

T

R

V

I

N

N

Y

S

P

P

-

N

-

S

P

K

V

V

N

N

A

T

L

L

G

S

Q

K

A

E

V

L

R

H

S

S

G

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L

F

L

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A

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A

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L

M

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N

Q

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G

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Q

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K

A

D

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D

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A

A

L

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L

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L

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F

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A

A

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G

A

A

D

D

I

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N

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M

F

L

G

A

-

A

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C

K

K

P

T

M

L

Q

Q

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E

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L

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-

Q

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E

-

A

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L

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D

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V282 (= V220) to D: in MTPD1; mild phenotype with slowly progressive myopathy and sensorimotor polyneuropathy; dbSNP:rs137852773
I305 (≠ V242) to N: in MTPD1; mild phenotype with slowly progressive myopathy and sensorimotor polyneuropathy; dbSNP:rs137852774
L342 (≠ S279) to P: in LCHAD deficiency; dbSNP:rs137852772
E510 (= E450) active site, For hydroxyacyl-coenzyme A dehydrogenase activity; to Q: in AFLP and LCHAD deficiency; loss of long-chain-3-hydroxyacyl-CoA dehydrogenase activity; dbSNP:rs137852769

1wdlA Fatty acid beta-oxidation multienzyme complex from pseudomonas fragi, form ii (native4) (see paper)
33% identity, 96% coverage: 28:699/702 of query aligns to 29:713/715 of 1wdlA

query
sites
1wdlA

V

V

N

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A

A

L

K

L

L

E

R

K

E

L

M

V

A

A

K

E

N

G

G

R

Q

K

S

F

F

active site: A69 (= A68), N89 (≠ T88), N93 (vs. gap), G117 (= G107), E120 (= E110), P139 (= P129), E140 (= E130), P147 (= P137), G148 (= G138), S430 (= S417), H451 (= H438), E463 (= E450), N501 (= N488)
binding nicotinamide-adenine-dinucleotide: A322 (= A309), I324 (≠ T311), M325 (= M312), D344 (≠ E331), I345 (≠ V332), A400 (= A387), V401 (= V388), E403 (= E390), N428 (= N415), T429 (= T416), S430 (= S417)

P28793 Fatty acid oxidation complex subunit alpha; EC 4.2.1.17; EC 5.1.2.3; EC 5.3.3.8; EC 1.1.1.35 from Pseudomonas fragi (see paper)
33% identity, 96% coverage: 28:699/702 of query aligns to 29:713/715 of P28793

query
sites
P28793

V

V

N

N

A

K

L

F

G

N

Q

R

A

L

V

T

R

L

S

N

G

E

L

L

L

R

A

Q

A

A

L

V

E

D

Q

A

G

I

Q

K

K

A

D

D

T

A

E

S

A

V

R

K

A

G

L

V

L

I

L

V

V

S

C

S

E

G

G

K

R

D

T

V

F

F

I

I

A

V

G

G

A

A

D

D

I

I

R

T

E

E

F

F

G

V

K

E

P

N

M

F

Q

K

E

L

P

P

T

D

L

A

P

E

T

L

L

I

V

A

N

G

T

N

-

L

-

E

-

A

-

N

-

K

-

I

-

F

-

S

-

D

F

F

E

E

N

D

S

L

D

N

K

V

P

P

L

T

V

V

A

A

A

A

I

I

H

N

G

G

T

I

A

A

L

L

G

G

G

G

G

G

L

L

E

E

T

M

A

C

L

L

S

A

C

A

H

D

Y

F

R

R

V

V

A

M

I

A

S

D

S

S

A

A

K

K

V

I

G

G

L

L

P

P

E

E

V

V

K

K

L

L

G

G

L

I

L

Y

P

P

G

G

A

F

G

G

G

G

T

T

Q

V

R

R

L

L

P

P

R

R

L

L

T

I

G

G

A

V

R

D

K

N

A

A

L

V

E

E

M

W

I

I

T

A

T

S

G

G

E

K

F

E

V

N

G

R

A

A

K

E

D

D

A

A

L

L

A

K

L

V

G

S

I

A

L

V

D

D

A

A

V

V

E

V

E

T

G

A

D

D

D

K

I

L

R

G

A

A

V

A

G

A

M

L

A

D

Y

L

A

I

Q

K

K

R

V

A

V

I

D

S

-

G

-

E

-

L

E

D

G

Y

K

K

P

A

V

K

R

R

R

Q

V

P

R

K

D

L

I

E

T

K

D

L

K

K

I

L

E

N

A

A

D

I

K

E

G

Q

S

M

D

M

V

A

F

F

D

E

Q

T

F

A

R

K

D

G

E

F

L

V

K

A

K

G

R

Q

A

A

R

G

G

P

L

N

F

Y

-

P

S

A

P

P

F

V

K

E

C

A

V

I

D

K

A

T

V

I

E

Q

A

K

A

A

F

A

N

N

L

F

P

G

F

R

D

D

E

K

G

A

M

L

K

E

R

V

E

E

R

A

E

A

L

G

F

F

M

A

E

K

C

L

M

A

E

K

S

T

P

S

Q

A

R

S

A

N

G

C

L

L

I

I

H

G

S

L

F

F

F

L

G

N

E
x
D

R

Q

E

E

V

L

S

K

K

K

V

-

K

K

G

A

L

K

S

V

K

Y

D

D

T

K

P

I

V

A

R

K

D

D

V

V

K

K

S

Q

V

A

G

A

I

V

I

L

G

G

A

A

G

G

T

I

M
|
M

G

G

G

G

G

G

I

I

A

A

M

Y

N

Q

F

S

V

A

N

S

V

K

G

G

I

T

P

P

V

I

T

L

I

M

V

K

E
x
D

V

I

K

N

Q

E

E

H

A

G

L

I

D

E

K

Q

G

G

L

L

A

A

I

E

I

A

R

A

R

K

N

L

Y

L

E

V

N

G

S

R

A

V

K

D

K

K

G

G

K

R

L

M

T

T

Q

P

E

A

Q

K

V

M

E

A

Q

E

R

V

M

L

A

N

L

G

I

I

T

R

P

P

S

T

L

L

T

S

Y

Y

D

G

D

D

F

F

R

G

D

N

V

V

D

D

L

L

V

V

I

V

E

E

A

A

V
|
V

F
x
V

E
|
E

N

N

M

P

A

K

I

V

K
|
K

K

Q

E

A

I

V

F

L

A

A

K

E

L

V

D

E

E

N

V

H

C

V

K

R

P

E

G

D

A

A

I

I

L

L

A

A

S

S

N

N

T

T

S
|
S

T

T

L

I

D

S

I

I

D

S

E

L

I

L

A

A

S

K

A

A

T

L

K

K

R

R

P

P

E

E

D

N

V

F

V

V

G

G

M

M

H

H

F

F

F

F

S
x
N

P

P

A

V

N

H

V

M

M

M

K

P

L

L

L

V

E

E

N

V

V

I

R

R

G

G

S

E

K

K

T

S

S

S

D

D

E

L

V

A

K

V

A

A

T

T

V

T

M

V

A

A

V

Y

A

A

K

K

K

K

I

M

K

G

K

K

V

N

G

P

V

I

M

V

V

V

G

N

N

D

C

C

Y

P

G

G

F

F

V

L

G

V

N
|
N

R

R

M

V

L

L

H

F

K

P

R

Y

G

F

T

G

E

G

A

F

M

A

S

K

L

L

V

V

D

S

E

A

G

G

A

V

T

D

P

F

Q

V

Q

R

V

I

D

D

K

K

V

V

L

M

T

E

D

K

L

F

G

G

F

W

P

P

M

M

G

G

Q

P

F

A

A

Y

M

L

S

M

D

D

L

V

A

V

G

G

I

I

D

D

V

T

G

G

Y

H

-

H

-

G

-

R

-

D

-

V

-

M

-

A

-

E

-

G

-

F

-

P

-

D

R

R

I

M

R

K

E

D

E

D

R

R

R

R

K

S

A

A

G

-

E

-

D

-

I

-

P

-

A

-

S

-

W

-

M

I

D

D

K

A

L

L

A

Y

E

E

Q

A

G

K

R

R

L

L

G

G

Q

Q

K

K

T

N

Q

G

A

K

G

G

V

F

Y

Y

K

A

Y

Y

E

E

-

A

-

D

-

K

E

K

G

G

S

K

R

Q

K

K

P

K

I

L

P

V

D

D

P

S

E

S

V

V

E

L

Q

E

L

V

I

L

E

K

Q

P

F

I

R

V

K

Y

E

E

Q

Q

G

-

I

-

T

-

P

-

R

R

E

D

I

V

T

T

N

D

Q

E

E

D

I

I

L

I

E

N

R

W

C

M

V

M

Y

I

V

P

M

L

I

C

N

L

E

E

G

T

A

V

K

R

I

C

L

L

E

E

E

D

G

G

I

I

A

V

D

E

R

T

P

A

L

A

D

E

I

A

D

D

I

M

V

G

W

L

I

V

Y
|
Y

G

G

Y

I

G

G

F

F

P

P

A

L

Y

F

R

R

G

G

G

G

P

A

M

L

F

R

W

Y

A

I

D

D

Q

S

E

I

G

G

L

V

D

A

T

E

I

F

L

V

S

A

A

L

V

A

K

D

K

Q

Y

Y

Q

A

D

E

T

L

V

-

G

-

G

G

E

A

Q

L

W

Y

E

H

P

P

A

T

A

A

L

K

L

L

E

R

K

E

L

M

V

A

A

K

E

N

G

G

R

Q

K

S

F

F

D297 (≠ E283) binding substrate
M325 (= M312) binding NAD(+)
D344 (≠ E331) binding NAD(+)
VVE 401:403 (≠ VFE 388:390) binding NAD(+)
K408 (= K395) binding NAD(+)
S430 (= S417) binding NAD(+)
N454 (≠ S441) binding NAD(+)
N501 (= N488) binding substrate
Y660 (= Y644) binding substrate

1wdmA Fatty acid beta-oxidation multienzyme complex from pseudomonas fragi, form i (native3) (see paper)
33% identity, 96% coverage: 28:699/702 of query aligns to 29:705/707 of 1wdmA

query
sites
1wdmA

V

V

N

N

A

K

L

F

G

N

Q

R

A

L

V

T

R

L

S

N

G

E

L

L

L

R

A

Q

A

A

L

V

E

D

Q

A

G

I

Q

K

K

A

D

D

T

A

E

S

A

V

R

K

A

G

L

V

L

I

L

V

V

S

C

S

E

G

G

K

R

D

T

V

F

F

I

I

A

V

G

G

A
|
A

D

D

I

I

R

T

E

E

F

F

G

V

K

E

P

N

M

F

Q

K

E

L

P

P

T

D

L

A

P

E

T

L

L

I

V

A

-

G

-
x
N

-

L

-

E

-

A

-
x
N

-

K

-

I

-

F

N

S

T

D

F

F

E

E

N

D

S

L

D

N

K

V

P

P

L

T

V

V

A

A

A

A

I

I

H

N

G

G

T

I

A

A

L

L

G

G

G
|
G

G

G

L

L

E
|
E

T

M

A

C

L

L

S

A

C

A

H

D

Y

F

R

R

V

V

A

M

I

A

S

D

S

S

A

A

K

K

V

I

G

G

L

L

P
|
P

E
|
E

V

V

K
|
K

L

L

G

G

L

I

L

Y

P
|
P

G
|
G

A

F

G

G

G

G

T

T

Q

V

R

R

L

L

P

P

R

R

L

L

T

I

G

G

A

V

R

D

K

N

A

A

L

V

E

E

M

W

I

I

T

A

T

S

G

G

E

K

F

E

V

N

G

R

A

A

K

E

D

D

A

A

L

L

A

K

L

V

G

S

I

A

L

V

D

D

A

A

V

V

E

V

E

T

G

A

D

D

D

K

I

L

R

G

A

A

V

A

G

A

M

L

A

D

Y

L

A

I

Q

K

K

R

V

A

V

I

D

S

-

G

-

E

-

L

E

D

G

Y

K

K

P

A

V

K

R

R

R

Q

V

P

R

K

D

L

I

E

T

K

D

L

K

K

I

L

E

N

A

A

D

I

K

E

G

Q

S

M

D

M

V

A

F

F

D

E

Q

T

F

A

R

K

D

G

E

F

L

V

K

A

K

G

R

Q

A

A

R

G

G

P

L

N

F

Y

-

P

S

A

P

P

F

V

K

E

C

A

V

I

D

K

A

T

V

I

E

Q

A

K

A

A

F

A

N

N

L

F

P

G

F

R

D

D

E

K

G

A

M

L

K

E

R

V

E

E

R

A

E

A

L

G

F

F

M

A

E

K

C

L

M

A

E

K

S

T

P

S

Q

A

R

S

A

N

G

C

L

L

I

I

H

G

S

L

F

F

F

L

G

N

E
x
D

R

Q

E

E

V

L

S

K

K

K

V

-

K

K

G

A

L

K

S

V

K

Y

D

D

T

K

P

I

V

A

R

K

D

D

V

V

K

K

S

Q

V

A

G

A

I

V

I

L

G
|
G

A
|
A

G

G

T
x
I

M
|
M

G

G

G

G

G

G

I

I

A

A

M

Y

N

Q

F

S

V

A

N

S

V

K

G

G

I

T

P

P

V

I

T

L

I

M

V

K

E
x
D

V

I

K

N

Q

E

E

H

A

G

L

I

D

E

K

Q

G

G

L

L

A

A

I

E

I

A

R

A

R

K

N

L

Y

L

E

V

N

G

S

R

A

V

K

D

K

K

G

G

K

R

L

M

T

T

Q

P

E

A

Q

K

V

M

E

A

Q

E

R

V

M

L

A

N

L

G

I

I

T

R

P

P

S

T

L

L

T

S

Y

Y

D

G

D

D

F

F

R

G

D

N

V

V

D

D

L

L

V

V

I

V

E

E

A

A

V
|
V

F

V

E
|
E

N

N

M

P

A

K

I

V

K

K

K

Q

E

A

I

V

F

L

A

A

K

E

L

V

D

E

E

N

V

H

C

V

K

R

P

E

G

D

A

A

I

I

L

L

A

A

S

S

N
|
N

T

T

S
|
S

T

T

L

I

D

S

I

I

D

S

E

L

I

L

A

A

S

K

A

A

T

L

K

K

R

R

P

P

E

E

D

N

V

F

V

V

G

G

M

M

H
|
H

F

F

F

F

S
x
N

P

P

A

V

N

H

V

M

M
|
M

K

P

L

L

L

V

E
|
E

N

V

V

I

R

R

G

G

S

E

K

K

T

S

S

S

D

D

E

L

V

A

K

V

A

A

T

T

V

T

M

V

A

A

V

Y

A

A

K

K

K

K

I

M

K

G

K

K

V

N

G

P

V

I

M

V

V

V

G

N

N

D

C

C

Y

P

G

G

F

F

V

L

G

V

N
|
N

R

R

M

V

L

L

H

F

K

P

R

Y

G

F

T

G

E

G

A

F

M

A

S

K

L

L

V

V

D

S

E

A

G

G

A

V

T

D

P

F

Q

V

Q

R

V

I

D

D

K

K

V

V

L

M

T

E

D

K

L

F

G

G

F

W

P
|
P

M

M

G

G

Q

P

F

A

A

Y

M

L

S

M

D

D

L

V

A

V

G

G

I

I

D

D

V

T

G

G

Y

H

-

H

-

G

-

R

-

D

-

V

-

M

-

A

-

E

-

G

-

F

-

P

-

D

R

R

I

M

R

K

E

D

E

D

R

R

R

R

K

S

A

A

G

-

E

-

D

-

I

-

P

-

A

-

S

-

W

-

M

I

D

D

K

A

L

L

A

Y

E

E

Q

A

G

K

R

R

L

L

G

G

Q

Q

K

K

T

N

Q

G

A

K

G

G

V

F

Y

Y

K

A

Y

Y

E

-

E

-

G

-

S

-

R

-

K

K

P

K

I

L

P

V

D

D

P

S

E

S

V

V

E

L

Q

E

L

V

I

L

E

K

Q

P

F

I

R

V

K

Y

E

E

Q

Q

G

-

I

-

T

-

P

-

R

R

E

D

I

V

T

T

N

D

Q

E

E

D

I

I

L

I

E

N

R

W

C

M

V

M

Y

I

V

P

M

L

I

C

N

L

E

E

G

T

A

V

K

R

I

C

L

L

E

E

E

D

G

G

I

I

A

V

D

E

R

T

P

A

L

A

D

E

I

A

D

D

I

M

V

G

W

L

I

V

Y
|
Y

G

G

Y

I

G

G

F

F

P

P

A
x
L

Y

F

R

R

G

G

G

G

P

A

M

L

F

R

W

Y

A

I

D

D

Q

S

E

I

G

G

L

V

D

A

T

E

I

F

L

V

S

A

A

L

V

A

K

D

K

Q

Y

Y

Q

A

D

E

T

L

V

-

G

-

G

G

E

A

Q

L

W

Y

E

H

P

P

A

T

A

A

L

K

L

L

E

R

K

E

L

M

V

A

A

K

E

N

G

G

R

Q

K

S

F

F

active site: A69 (= A68), N89 (vs. gap), N93 (vs. gap), G117 (= G107), E120 (= E110), P139 (= P129), E140 (= E130), P147 (= P137), G148 (= G138), S430 (= S417), H451 (= H438), E463 (= E450), N501 (= N488)
binding acetyl coenzyme *a: K142 (= K132), D297 (≠ E283), M459 (= M446), N501 (= N488), P534 (= P521), Y652 (= Y644), L658 (≠ A650)
binding nicotinamide-adenine-dinucleotide: G321 (= G308), A322 (= A309), I324 (≠ T311), M325 (= M312), D344 (≠ E331), V401 (= V388), E403 (= E390), N428 (= N415), S430 (= S417), N454 (≠ S441)

P21177 Fatty acid oxidation complex subunit alpha; EC 4.2.1.17; EC 5.1.2.3; EC 5.3.3.8; EC 1.1.1.35 from Escherichia coli (strain K12) (see 2 papers)
33% identity, 92% coverage: 28:675/702 of query aligns to 28:691/729 of P21177

query
sites
P21177

V

V

N

N

A

K

L

L

G

D

Q

T

A

A

V

T

R

V

S

A

G

S

L

L

L

G

A

E

A

A

L

I

E

G

Q

V

G

L

Q

E

K

Q

D

Q

T

S

E

D

A

L

R

K

A

G

L

L

L

L

L

L

V

R

C

S

E

N

G

K

R

A

T

A

F

F

I

I

A

V

G

G

A

A

D

D

I

I

R

T

E

E

F

F

G

L

K

S

P

L

M

F

Q

L

E

V

P

P

T

E

-

E

-

Q

-

L

-

S

-

Q

-

W

-

L

-

H

-

F

L

A

P

N

T

S

L

V

V

F

N

N

T

R

F

L

E

E

N

D

S

L

D

P

K

V

P

P

L

T

V

I

A

A

A

A

I

V

H

N

G

G

T

Y

A

A

L

L

G

G

G
|
G

G

G

L

C

E

E

T

C

A

V

L

L

S

A

C

T

H

D

Y

Y

R

R

V

L

A

A

I

T

S

P

S

D

A

L

K

R

V

I

G

G

L

L

P

P

E

E

V

T

K

K

L

L

G

G

L

I

L

M

P

P

G

G

A

F

G

G

G

G

T

S

Q

V

R

R

L

M

P

P

R

R

L

M

T

L

G

G

A

A

R

D

K

S

A

A

L

L

E

E

M

I

I

I

T

A

T

A

G

G

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K

F

D

V

V

G

G

A

A

K

D

D

Q

A

A

L

L

A

K

L

I

G

G

I

L

L

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D

D

A

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V

V

E

V

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K

G

A

D

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D

K

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V

A

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G

A

M

K

A

A

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A

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Q

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Q

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A

V

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D

N

-

G

-

D

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L

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W

K

K

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R

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K

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A

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K

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G

A

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D

M

V

S

F

F

D

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Q

I

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A

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K

D

G

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M

L

V

K

A

K

Q

R

T

A

A

R

G

G

K

L

H

F

Y

-

P

S

A

P

P

F

I

K

T

C

A

V

V

D

K

A

T

V

I

E

E

A

A

A

A

F

A

N

R

L

F

P

G

F

R

D

E

E

E

G

A

M

L

K

N

R

L

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F

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A

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A

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L

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F

F

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N

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R

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V

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K

-

G

K

K

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A

K

K

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K

L

L

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K

K

D

D

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-

P

-

V

V

R

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D

T

V

P

K

K

S

Q

V

A

G

A

I

V

I

L

G

G

A

A

G
|
G

T

I

M

M

G

G

G

G

G

G

I

I

A

A

M

Y

N

Q

F

S

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A

N

W

V

K

G

G

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P

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V

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V

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V

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K

N

Q

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L

L

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K

L

G

G

L

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A

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A

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N

L

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L

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N

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A

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K

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G

K

K

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P

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L

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Y

Y

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A

D

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F

F

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D

D

R

V

V

D

D

L

I

V

V

I

V

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A

A

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V

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V

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N

N

M

P

A

K

I

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K

K

K

K

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A

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F

L

A

A

K

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L

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D

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E

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V

K

C

V

K

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P

Q

G

D

A

T

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V

L

L

A

A

S

S

N

N

T

T

S

S

T

T

L

I

D

P

I

I

D

S

E

E

I

L

A

A

S

N

A

A

T

L

K

E

R

R

P

P

E

E

D

N

V

F

V

C

G

G

M

M

H
|
H

F

F

F

F

S

N

P

P

A

V

N

H

V

R

M

M

K

P

L

L

L

V

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N

I

V

I

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R

G

G

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K

K

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S

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D

D

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T

K

I

A

A

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K

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V

M

V

A

A

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W

A

A

K

S

K

K

I

M

K

G

K

K

V

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G

P

V

I

M

V

V

V

G

N

N

D

C

C

Y

P

G

G

F

F

V

F

G

V

N

N

R

R

M

V

L

L

H

F

K

P

R

Y

G

F

T

A

E

G

A

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M

S

S

Q

L

L

V

L

D

R

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D

G

G

A

A

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D

P

F

Q

R

Q

K

V

I

D

D

K

K

V

V

L

M

-

E

T

K

D

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L

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G

G

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P

P

M

M

G

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A

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M

L

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L

D

D

L

V

A

V

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G

I

I

D

D

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T

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A

Y

H

-

H

-

A

-

Q

-

A

-

V

-

M

-

A

-

A

-

G

-

F

-

P

-

Q

R

R

I

M

R

Q

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K

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D

R

Y

R

R

K

D

A

A

G

-

E

-

D

-

I

-

P

-

A

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S

-

W

-

M

I

D

D

K

A

L

L

A

F

E

D

Q

A

G

N

R

R

L

F

G

G

Q

Q

K

K

T

N

Q

G

A

L

G

G

V

F

Y

W

K

R

Y

Y

E

K

E

E

G

D

S

S

R

-

K

K

P

G

I

K

P

P

D

K

P

K

E

E

V

E

E

D

Q

A

L

A

I

V

E

E

Q

D

F

L

R

L

K

A

E

E

Q

V

G

S

I

Q

T

P

P

K

R

R

E

D

I

F

T

S

N

E

Q

E

E

E

I

I

L

I

E

A

R

R

C

M

V

M

Y

I

V

P

M

M

I

V

N

N

E

E

G

V

A

V

K

R

I

C

L

L

E

E

E

E

G

G

I

I

A

I

D

A

R

T

P

P

L

A

D

E

I

A

D

D

I

M

V

A

W

L

I

V

Y

Y

G

G

Y

L

G

G

F

F

P

P

A

P

Y

F

R

H

G

G

G

G

P

A

M

F

F

R

W

W

A

L

D

D

Q

T

E

L

G

G

L

S

D

A

T

K

I

Y

L

L

S

D

A

M

V

A

K

Q

K

Q

Y

Y

Q

Q

G116 (= G107) mutation to F: Absence of both enoyl-CoA hydratase and 3-hydroxyacyl-CoA epimerase activities. Delta(3)-cis-Delta(2)-trans-enoyl-CoA isomerase is only slightly affected.
G322 (= G310) mutation to A: 10-fold increase in KM for NADH.
H450 (= H438) active site, For 3-hydroxyacyl-CoA dehydrogenase activity; mutation H->A,Q: Almost complete loss of 3-hydroxyacyl-CoA dehydrogenase activity.

6tnmA E. Coli aerobic trifunctional enzyme subunit-alpha (see paper)
33% identity, 92% coverage: 28:675/702 of query aligns to 28:691/719 of 6tnmA

query
sites
6tnmA

V

V

N

N

A

K

L

L

G

D

Q

T

A

A

V

T

R

V

S

A

G

S

L

L

L

G

A

E

A

A

L

I

E

G

Q

V

G

L

Q

E

K

Q

D

Q

T

S

E

D

A

L

R

K

A

G

L

L

L

L

L

L

V

R

C

S

E

N

G

K

R

A

T

A

F

F

I

I

A

V

G

G

A
|
A

D

D

I

I

R

T

E

E

F
|
F

G

L

K

S

P

L

M

F

Q

L

E

V

P

P

T

E

-

E

-

Q

-

L

-

S

-

Q

-

W

-

L

-

H

-

F

L

A

P

N

T

S

L

V

V

F

N

N

T

R

F

L

E

E

N

D

S

L

D

P

K

V

P

P

L

T

V

I

A

A

A

A

I

V

H

N

G

G

T

Y

A

A

L

L

G

G

G
|
G

G

G

L

C

E
|
E

T

C

A

V

L

L

S

A

C

T

H

D

Y

Y

R

R

V

L

A

A

I

T

S

P

S

D

A

L

K

R

V

I

G

G

L

L

P
|
P

E
|
E

V

T

K

K

L

L

G

G

L

I

L

M

P

P

G
|
G

A

F

G

G

G

G

T

S

Q

V

R

R

L

M

P

P

R

R

L

M

T

L

G

G

A

A

R

D

K

S

A

A

L

L

E

E

M

I

I

I

T

A

T

A

G

G

E

K

F

D

V

V

G

G

A

A

K

D

D

Q

A

A

L

L

A

K

L

I

G

G

I

L

L

V

D

D

A

G

V

V

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V

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K

G

A

D

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D

K

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L

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A

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G

A

M

K

A

A

Y

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A

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Q

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K

Q

V

A

V

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D

N

-

G

-

D

-

L

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D

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W

K

K

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A

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K

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R

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D

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K

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A

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K

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A

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M

V

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F

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K

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L

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K

A

K

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A

A

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G

K

L

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F

Y

-

P

S

A

P

P

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A

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V

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K

A

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A

A

A

A

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A

N

R

L

F

P

G

F

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D

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E

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G

A

M

L

K
x
N

R

L

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F

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A

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-

G

K

K

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A

K

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K

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L

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K

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D

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P

-

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V

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K

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M

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G

G

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P

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x
D

V
x
I

K

N

Q

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K

A

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L

L

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K

L

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G

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A

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A

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N

L

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L

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N

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A

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G

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L

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V

I

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A

V
|
V

F
x
V

E

E

N

N

M

P

A

K

I
x
V

K

K

K

K

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A

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A

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L

L

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A

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S

N

N

T

T

S
|
S

T

T

L

I

D

P

I

I

D

S

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E

I

L

A

A

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N

A

A

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L

K

E

R

R

P

P

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D

N

V

F

V

C

G

G

M

M

H
|
H

F

F

F

F

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N

P

P

A

V

N

H

V

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M

M

K

P

L

L

L

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|
E

N

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G

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K

K

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S

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D

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V

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A

A

V

W

A

A

K

S

K

K

I

M

K

G

K

K

V

T

G

P

V

I

M

V

V

V

G

N

N

D

C

C

Y

P

G

G

F

F

V

F

G

V

N
|
N

R

R

M

V

L

L

H

F

K

P

R

Y

G

F

T

A

E

G

A

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L

L

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L

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A

V

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G

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I

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D

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H

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-

A

-

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A

-

A

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M

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A

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L

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R

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|
K

T

N

Q

G

A

L

G

G

V

F

Y

W

K

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Y

Y

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K

E

E

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K

K

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L

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I

L

I

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A

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M

Y

I

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P

M

M

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N

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E

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V

A

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L

L

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I

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P

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A

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A

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L

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Y

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G

Y

L

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G

G

G

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A

M

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F

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W

W

A

L

D

D

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G

G

L

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D

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L

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Y

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active site: A68 (= A68), F73 (= F73), G116 (= G107), E119 (= E110), P138 (= P129), E139 (= E130), G147 (= G138), N271 (≠ K258), S429 (= S417), H450 (= H438), E462 (= E450), N500 (= N488)
binding adenosine-5'-triphosphate: D343 (≠ E331), I344 (≠ V332), V400 (= V388), V401 (≠ F389), V406 (≠ I394), K584 (= K567)

6yswA E. Coli anaerobic trifunctional enzyme subunit-alpha in complex with coenzyme a
34% identity, 98% coverage: 14:699/702 of query aligns to 9:702/707 of 6yswA

query
sites
6yswA

R

R

E

L

G

D

N

N

I

I

G

A

V

V

I

I

T

T

V

I

N

D

Y

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P

P

-

G

-
x
E

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V
x
M

N

N

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L

L

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A

A

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A

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L

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F

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A
|
A

D
|
D

I
|
I

R

N

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M

F
x
I

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G

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N

-

C

K

K

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A

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Q

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E

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A

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L

A

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L

-
x
A

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L

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G

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|
G

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|
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|
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|
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K

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N

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|
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|
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|
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|
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I

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D

D

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L

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I

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T

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N

A

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P

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Q

I

V

L

A

E

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R

R

C

C

V

V

Y

M

V

L

M

M

I

L

N

N

E

E

G

A

A

V

K

R

I

C

L

V

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D

E

E

G

Q

I

V

A

I

D

R

R

S

P

V

L

R

D

D

I

G

D

D

I

I

V

G

W

A

I

V

Y

F

G

G

Y

I

G

G

F

F

P

P

A

P

Y

F

R

L

G

G

G

G

P

P

M

F

F

R

W

Y

A

I

D

D

Q

S

E

L

G

G

L

A

D

G

T

E

I

V

L

V

S

A

A

I

V

M

K

Q

K

R

Y

L

Q

A

D

-

T

T

V

Q

G

Y

G

G

E

S

Q

R

W

F

E

T

P

P

A

C

A

E

L

R

L

L

E

V

K

E

L

M

V

G

A

A

E

R

G

G

R

E

K

S

F

F

active site: A66 (= A68), I71 (≠ F73), A84 (vs. gap), Q88 (≠ P83), G112 (= G107), E115 (= E110), P136 (= P129), E137 (= E130), G145 (= G138), D264 (≠ K258), S422 (= S417), H443 (= H438), E455 (= E450), N493 (= N488)
binding coenzyme a: E23 (vs. gap), M25 (≠ V28), A66 (= A68), D67 (= D69), I68 (= I70), P136 (= P129), E137 (= E130), L140 (= L133), T290 (≠ R284), K293 (≠ S287)

7o4uA Structure of the alpha subunit of mycobacterium tuberculosis beta- oxidation trifunctional enzyme in complex with oxidized nicotinamide adenine dinucleotide (see paper)
31% identity, 85% coverage: 55:654/702 of query aligns to 53:669/711 of 7o4uA

query
sites
7o4uA

L

V

L

V

L

V

V

A

C

S

E

A

G

K

R

K

T

T

F

F

I

F

A

A

G

G

A

G

D

D

I

V

R

K

E

T

F

M

G

I

K

Q

P

A

M

R

Q

P

E

E

-

D

-

A

-

G

-

D

-

V

-

F

-

N

-

T

-

V

P

E

T

T

L

I

P

K

T

R

L

Q

V

L

N

R

T

T

F

L

E

E

N

T

S

L

D

G

K

K

P

P

L

V

V

V

A

A

A

A

I

I

H

N

G

G

T

A

A

A

L

L

G

G

G

G

G

G

L

L

E

E

T

I

A

A

L

L

S

A

C

C

H

H

Y

H

R

R

V

I

A

A

-

A

-

D

I

V

S

K

S

G

A

S

K

Q

V

L

G

G

L

L

P

P

E

E

V

V

K

T

L

L

G

G

L

L

L

L

P

P

G

G

A

G

G

G

G

G

T

V

Q

T

R

R

L

T

P

V

R

R

L

M

T

F

G

G

A

I

R

Q

K

N

A

A

-

F

L

V

E

S

M

V

I

L

T

A

T

Q

G

G

E

T

F

R

V

F

G

K

A

P

K

A

D

K

A

A

L

K

A

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L

I

G

G

I

L

L

V

D

D

A

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-

L

V

V

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A

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D

E

D

E

I

L

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V

A

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V

A

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A

M

K

A

A

Y

W

A

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Q

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K

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L

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K

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G

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L

G

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A
|
A

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G

T
x
M

M
|
M

G

G

G

A

G

G

I

I

A

A

M

Y

N

V

F

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V

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G

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D

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|
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K

S

Q

L

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A

A

L

A

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A

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K

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G

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G

V

V

D

D

L

F

V

V

I

I

E

E

A

A

V
|
V

F
|
F

E
|
E

N

N

M

Q

A

E

I

L

K

K

K

H

E

K

I

V

F

F

A

G

K

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L

I

D

E

E

D

V

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C

V

K

E

P

P

G

N

A

A

I

I

L

L

A

G

S

S

N
|
N

T

T

S

S

T

T

L

L

D

P

I

I

D

T

E

G

I

L

A

A

S

T

A

G

T

V

K

K

R

R

P

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D

D

V

F

V

I

G

G

M

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H
|
H

F

F

F

F

S

S

P

P

A

V

N

D

V

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M

M

K

P

L

L

L

V

E

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N

I

V

I

R

K

G

G

S

E

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K

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T

S

S

D

D

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A

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L

A

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V

V

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A

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V

Y

A

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L

K

A

I

I

K

G

K

K

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P

V

I

M

V

V

V

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N

N

D

C

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G

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N

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N

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S

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M

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D

G

E

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G

A

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A

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D

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V

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G

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A

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P

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P

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L

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M

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S

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D

D

L

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A

L

G

N

I

L

D

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V

L

G

M

Y

H

R

K

I

I

R

A

E

V

E

A

R

T

R

R

K

K

A

G

G

V

E

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D

D

I

A

P

G

A

G

S

T

W

Y

-

Q

-

P

-

H

-

P

-

A

-

E

-

A

-

V

M

V

D

E

K

K

L

M

A

I

E

E

Q

L

G

G

R

R

L

S

G

G

Q

R

K

L

T

K

Q

G

A

A

G

G

V

F

Y

Y

K

E

Y

Y

E

A

E

D

G

G

S

K

R

R

K

S

P

G

I

L

P

-

D

-

P

-

E

-

V

W

E

P

Q

G

L

L

I

R

E

E

Q

T

F

F

R

K

K

S

E

G

Q

S

G

S

I

Q

T

P

P

P

R

L

E

-

I

-

T

-

N

-

Q

Q

E

D

I

M

L

I

E

D

R

R

C

M

V

L

Y

F

V

A

M

E

I

A

N

L

E

E

G

T

A

Q

K

K

I

C

L

L

E

D

E

E

G

G

I

V

A

L

D

T

R

S

P

T

L

A

D

D

I

A

D

N

I

I

V

G

W

S

I

I

Y

M

G

G

Y

I

G

G

F

F

P

P

A

P

Y

W

R

T

G

G

G

G

binding nicotinamide-adenine-dinucleotide: A324 (= A309), M326 (≠ T311), M327 (= M312), D346 (≠ E331), V347 (= V332), V403 (= V388), F404 (= F389), E405 (= E390), N430 (= N415), H453 (= H438)

Query Sequence

>GFF4001 FitnessBrowser__Marino:GFF4001
MRGEIMSEVVSYNREGNIGVITVNYPPVNALGQAVRSGLLAALEQGQKDTEARALLLVCE
GRTFIAGADIREFGKPMQEPTLPTLVNTFENSDKPLVAAIHGTALGGGLETALSCHYRVA
ISSAKVGLPEVKLGLLPGAGGTQRLPRLTGARKALEMITTGEFVGAKDALALGILDAVEE
GDDIRAVGMAYAQKVVDEGKPVRRVRDITDKIEADKGSDVFDQFRDELKKRARGLFSPFK
CVDAVEAAFNLPFDEGMKRERELFMECMESPQRAGLIHSFFGEREVSKVKGLSKDTPVRD
VKSVGIIGAGTMGGGIAMNFVNVGIPVTIVEVKQEALDKGLAIIRRNYENSAKKGKLTQE
QVEQRMALITPSLTYDDFRDVDLVIEAVFENMAIKKEIFAKLDEVCKPGAILASNTSTLD
IDEIASATKRPEDVVGMHFFSPANVMKLLENVRGSKTSDEVKATVMAVAKKIKKVGVMVG
NCYGFVGNRMLHKRGTEAMSLVDEGATPQQVDKVLTDLGFPMGQFAMSDLAGIDVGYRIR
EERRKAGEDIPASWMDKLAEQGRLGQKTQAGVYKYEEGSRKPIPDPEVEQLIEQFRKEQG
ITPREITNQEILERCVYVMINEGAKILEEGIADRPLDIDIVWIYGYGFPAYRGGPMFWAD
QEGLDTILSAVKKYQDTVGGEQWEPAALLEKLVAEGRKFADL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory