SitesBLAST
Comparing GFF4002 FitnessBrowser__psRCH2:GFF4002 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
O59010 Glutamate transporter homolog; Glt(Ph); Sodium-aspartate symporter Glt(Ph); Sodium-dependent aspartate transporter from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see 3 papers)
47% identity, 96% coverage: 12:412/419 of query aligns to 5:423/425 of O59010
- S65 (= S66) mutation to V: Strongly decreased chloride conductance.
- R276 (≠ S270) mutation to S: Increased rate of aspartate transport; when associated with R-395.
- RSS 276:278 (≠ SSS 270:272) binding L-aspartate
- M311 (= M306) mutation to A: Decreased dependence of aspartate binding on Na(+) concentration.
- T314 (= T309) binding L-aspartate
- V355 (≠ I350) binding L-aspartate
- D394 (= D383) binding L-aspartate
- M395 (= M384) mutation to R: Increased rate of aspartate transport; when associated with S-276.
- R397 (= R386) mutation to A: Strongly decreased affinity for aspartate.
- N401 (= N390) binding L-aspartate
- D405 (= D394) mutation to N: Strongly decreased affinity for aspartate.
6bauA Crystal structure of gltph r397c in complex with l-cysteine (see paper)
47% identity, 93% coverage: 16:405/419 of query aligns to 1:408/408 of 6bauA
- binding cysteine: S270 (= S272), M303 (= M306), T306 (= T309), A345 (= A348), G346 (= G349), V347 (≠ I350), G351 (= G354), D386 (= D383), C389 (≠ R386), T390 (= T387), N393 (= N390)
6bavA Crystal structure of gltph r397c in complex with s-benzyl-l-cysteine (see paper)
47% identity, 93% coverage: 16:405/419 of query aligns to 1:408/409 of 6bavA
2nwwA Crystal structure of gltph in complex with tboa (see paper)
47% identity, 93% coverage: 18:405/419 of query aligns to 2:407/407 of 2nwwA
6x15A Inward-facing state of the glutamate transporter homologue gltph in complex with l-aspartate and sodium ions (see paper)
46% identity, 95% coverage: 12:408/419 of query aligns to 5:419/419 of 6x15A
- binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: Y7 (≠ L14), F46 (≠ I47), F46 (≠ I47), P75 (≠ S77), L91 (≠ V93), F95 (= F97), L130 (= L131), I133 (= I134), I159 (= I160), Y167 (≠ L168), K196 (= K189), G200 (≠ L193), I207 (= I200), F210 (= F203), L250 (= L243), I262 (≠ F256), M269 (≠ L263), T334 (≠ S329), V335 (≠ A330), G336 (= G331), T340 (≠ M335), L343 (= L338), M399 (≠ T388)
- binding aspartic acid: S277 (= S271), S278 (= S272), T314 (= T309), G354 (= G349), A358 (= A353), G359 (= G354), D394 (= D383), R397 (= R386), T398 (= T387)
- binding sodium ion: Y89 (= Y91), T92 (= T94), S93 (≠ T95), G306 (= G301), T308 (= T303), N310 (= N305), N310 (= N305), M311 (= M306), D312 (= D307), S349 (= S344), I350 (= I345), T352 (= T347), N401 (= N390), V402 (= V391), D405 (= D394)
Sites not aligning to the query:
6x14A Inward-facing state of the glutamate transporter homologue gltph in complex with tfb-tboa (see paper)
47% identity, 94% coverage: 12:405/419 of query aligns to 2:413/413 of 6x14A
- binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: Y4 (≠ L14), G66 (= G70), V83 (≠ I88), I157 (≠ A161), Y164 (≠ L168), K193 (= K189), T305 (= T303), I306 (= I304), I347 (= I345)
- binding (2~{S},3~{S})-2-azanyl-3-[[3-[[4-(trifluoromethyl)phenyl]carbonylamino]phenyl]methoxy]butanedioic acid: I13 (= I23), M199 (= M195), S275 (= S272), T311 (= T309), G356 (= G354), L384 (≠ A376), D391 (= D383), R394 (= R386)
5e9sA Crystal structure of substrate-bound glutamate transporter homologue glttk (see paper)
44% identity, 97% coverage: 11:416/419 of query aligns to 2:427/427 of 5e9sA
- binding aspartic acid: R274 (≠ S270), S275 (= S271), S276 (= S272), T313 (= T309), G353 (= G349), V354 (≠ I350), A357 (= A353), G358 (= G354), D394 (= D383), R397 (= R386), T398 (= T387)
- binding decyl-beta-d-maltopyranoside: L194 (≠ K189), G198 (≠ L193), Y202 (≠ V197)
- binding sodium ion: Y87 (= Y91), T90 (= T94), S91 (≠ T95), S276 (= S272), G305 (= G301), A306 (= A302), T307 (= T303), N309 (= N305), N309 (= N305), M310 (= M306), D311 (= D307), S348 (= S344), I349 (= I345), G350 (= G346), T351 (= T347), N401 (= N390), V402 (= V391), D405 (= D394)
6xwnB Structure of glutamate transporter homologue glttk in the presence of tboa inhibitor (see paper)
44% identity, 97% coverage: 11:415/419 of query aligns to 2:426/426 of 6xwnB
6zgbA Glutamate transporter homologue glttk in complex with a photo cage compound (see paper)
44% identity, 97% coverage: 12:416/419 of query aligns to 1:425/425 of 6zgbA
6zl4A The structure of glutamate transporter homologue glttk in complex with the photo switchable compound (cis) (see paper)
44% identity, 95% coverage: 18:416/419 of query aligns to 6:424/424 of 6zl4A
- binding decyl-beta-d-maltopyranoside: L191 (≠ K189), G195 (≠ L193), R282 (≠ E281)
- binding (2~{S},3~{S})-2-azanyl-3-[[4-[2-(4-methoxyphenyl)hydrazinyl]phenyl]methoxy]butanedioic acid: R271 (≠ S270), S272 (= S271), S273 (= S272), M307 (= M306), T310 (= T309), G353 (= G352), A354 (= A353), R394 (= R386), T395 (= T387)
Sites not aligning to the query:
6r7rA Crystal structure of the glutamate transporter homologue glttk in complex with d-aspartate (see paper)
44% identity, 95% coverage: 18:416/419 of query aligns to 2:416/416 of 6r7rA