SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF4002 Psest_4075 Na+/H+-dicarboxylate symporters to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

O59010 Glutamate transporter homolog; Glt(Ph); Sodium-aspartate symporter Glt(Ph); Sodium-dependent aspartate transporter from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see 3 papers)
47% identity, 96% coverage: 12:412/419 of query aligns to 5:423/425 of O59010

query
sites
O59010

R

R

L

K

L

Y

V

I

R

E

L

Y

P

P

L

V

W

L

Q

Q

Q

K

I

I

L

L

I

I

G

G

L

L

A

I

L

L

G

G

V

A

A

I

A

V

G

G

M

L

A

I

F

L

G

G

-

H

-

Y

-

G

-

Y

A

A

D

D

A

A

-

V

-

K

Q

T

L

Y

L

V

A

K

P

P

I

F

G

G

T

D

L

L

F

F

L

V

N

R

A

L

I

L

K

K

M

M

L

L

I

V

V

M

P

P

L

I

V

V

F

F

V

A

S
|
S

L

L

V

V

A

V

G

G

I

A

T

A

S

S

M

I

Q

-

D

S

S

P

A

A

K

R

L

L

G

G

R

R

I

V

S

G

L

V

K

K

T

I

I

V

A

V

I

Y

Y

Y

L

L

V

L

T

T

T

S

A

A

F

F

A

A

V

V

S

T

I

L

G

G

L

I

F

M

G

A

A

R

L

L

F

F

S

N

P

P

G

G

E

A

G

G

M

I

N

H

M

L

V

A

A

V

S

G

G

G

N

Q

E

Q

-

F

Q

Q

A

P

K

K

Q

Q

A

A

P

P

S

P

L

L

V

V

S

K

I

I

L

L

V

L

G

D

L

I

V

V

P

P

A

T

N

N

P

P

V

F

T

G

A

A

F

L

A

A

E

N

G

G

N

Q

I

V

L

L

Q

P

I

T

I

I

V

F

F

F

A

A

I

I

A

I

L

L

G

G

V

I

S

A

I

I

N

T

L

Y

I

L

G

M

E

N

R

S

G

E

A

N

P

E

A

K

V

V

R

R

-

K

-

S

-

A

-

E

-

T

L

L

F

L

D

D

A

A

-

I

-

N

-

G

L

L

A

A

E

E

T

A

F

M

Y

Y

K

K

L

I

T

V

D

N

L

G

V

V

M

M

R

Q

V

Y

A

A

P

P

I

I

G

G

V

V

F

F

A

A

L

L

T

I

A

A

G

Y

V

V

V

M

G

A

S

E

H

Q

G

G

A

V

E

K

V

V

L

V

L

G

P

E

L

L

A

A

G

K

V

V

I

T

G

A

V

A

I

V

Y

Y

L

V

A

G

S

L

I

T

A

L

H

Q

V

I

L

L

V

V

Y

Y

G

F

G

V

L

L

G

K

L

I

L

Y

A

G

R

-

L

I

N

D

P

P

L

I

R

S

F

F

F

I

Q

K

G

K

I

A

A

K

P

D

A

A

L

M

A

L

V

T

A

A

F

F

S

V

T

T

S
x
R

S
|
S

S

S

G

G

T

T

L

L

P

P

V

V

S

T

I

M

E

R

C

V

A

A

R

-

K

K

N

E

L

M

G

G

V

I

S

S

E

E

G

G

V

I

A

Y

G

S

F

F

V

T

L

L

P

P

V

L

G

G

A

A

T

T

I

I

N

N

M
|
M

D

D

G

G

T
|
T

A

A

I

L

Y

Y

Q

Q

G

G

V

V

L

C

A

T

L

F

F

F

I

I

A

A

Q

N

A

A

F

L

G

G

I

S

D

H

L

L

S

T

A

V

G

G

Q

Q

Y

Q

A

L

M

T

I

I

I

V

L

L

T

T

A

A

T

V

L

L

A

A

S

S

I

I

G

G

T

T

A

A

G

G

I
x
V

P

P

G

G

A

A

G

G

L

A

I

I

M

M

L

L

G

A

L

M

V

V

L

L

T

E

A

S

A

V

G

G

L

L

P

P

L

L

E

T

-

D

-

P

-

N

-

V

-

A

G

A

V

Y

A

A

L

M

I

I

A

L

G

G

I

I

D

D

R

A

I

I

L

L

D
|
D

M
|
M

A

G

R
|
R

T

T

T

M

V

V

N
|
N

V

V

A

T

G

G

D
|
D

L

L

M

T

T

G

T

T

T

A

L

I

V

V

G

A

R

K

S

T

E

E

Q

G

E

E

L

L

D

E

R

K

A

G

I

V

S65 (= S66) mutation to V: Strongly decreased chloride conductance.
R276 (≠ S270) mutation to S: Increased rate of aspartate transport; when associated with R-395.
RSS 276:278 (≠ SSS 270:272) binding
M311 (= M306) mutation to A: Decreased dependence of aspartate binding on Na(+) concentration.
T314 (= T309) binding
V355 (≠ I350) binding
D394 (= D383) binding
M395 (= M384) mutation to R: Increased rate of aspartate transport; when associated with S-276.
R397 (= R386) mutation to A: Strongly decreased affinity for aspartate.
N401 (= N390) binding
D405 (= D394) mutation to N: Strongly decreased affinity for aspartate.

6bauA Crystal structure of gltph r397c in complex with l-cysteine (see paper)
47% identity, 93% coverage: 16:405/419 of query aligns to 1:408/408 of 6bauA

query
sites
6bauA

R

E

L

Y

P

P

L

V

W

L

Q

Q

Q

K

I

I

L

L

I

I

G

G

L

L

A

I

L

L

G

G

V

A

A

I

A

V

G

G

M

L

A

I

F

L

G

G

-

H

-

Y

-

G

-

Y

A

A

D

D

A

A

-

V

-

K

Q

T

L

Y

L

V

A

K

P

P

I

F

G

G

T

D

L

L

F

F

L

V

N

R

A

L

I

L

K

K

M

M

L

L

I

V

V

M

P

P

L

I

V

V

F

F

V

A

S

S

L

L

V

V

A

V

G

G

I

A

T

A

S

S

M

I

Q

-

D

S

S

P

A

A

K

R

L

L

G

G

R

R

I

V

S

G

L

V

K

K

T

I

I

V

A

V

I

Y

Y

Y

L

L

V

L

T

T

T

S

A

A

F

F

A

A

V

V

S

T

I

L

G

G

L

I

F

M

G

A

A

R

L

L

F

F

S

N

P

P

G

G

E

A

G

G

M

I

N

H

M

L

V

A

A

V

S

G

G

G

N

Q

E

Q

-

F

Q

Q

A

P

K

K

Q

Q

A

A

P

P

S

P

L

L

V

V

S

K

I

I

L

L

V

L

G

D

L

I

V

V

P

P

A

T

N

N

P

P

V

F

T

G

A

A

F

L

A

A

E

N

G

G

N

Q

I

V

L

L

Q

P

I

T

I

I

V

F

F

F

A

A

I

I

A

I

L

L

G

G

V

I

S

A

I

I

N

T

L

Y

I

L

G

M

E

N

R

S

G

E

A

N

P

E

A

K

V

V

R

R

-

K

-

S

-

A

-

E

-

T

L

L

F

L

D

D

A

A

-

I

-

N

-

G

L

L

A

A

E

E

T

A

F

M

Y

Y

K

K

L

I

T

V

D

N

L

G

V

V

M

M

R

Q

V

Y

A

A

P

P

I

I

G

G

V

V

F

F

A

A

L

L

T

I

A

A

G

Y

V

V

V

M

G

A

S

E

H

Q

G

G

A

V

E

K

V

V

L

V

L

G

P

E

L

L

A

A

G

K

V

V

I

T

G

A

V

A

I

V

Y

Y

L

V

A

G

S

L

I

T

A

L

H

Q

V

I

L

L

V

V

Y

Y

G

F

G

V

L

L

G

K

L

I

L

Y

A

G

R

-

L

I

N

D

P

P

L

I

R

S

F

F

F

I

Q

K

G

K

I

A

A

K

P

D

A

A

L

M

A

L

V

T

A

A

F

F

S

V

T

T

S
x
R

S

S

S
|
S

S

S

G

G

T

T

L

L

P

P

V

V

S

T

I

M

E

R

C

V

A

A

R

-

K

K

N

E

L

M

G

G

V

I

S

S

E

E

G

G

V

I

A

Y

G

S

F

F

V

T

L

L

P

P

V

L

G

G

A

A

T

T

I

I

N

N

M
|
M

D

D

G

G

T
|
T

A

A

I

L

Y

Y

Q

Q

G

G

V

V

L

C

A

T

L

F

F

F

I

I

A

A

Q

N

A

A

F

L

G

G

I

S

D

H

L

L

S

T

A

V

G

G

Q

Q

Y

Q

A

L

M

T

I

I

I

V

L

L

T

T

A

A

T

V

L

L

A

A

S

S

I

I

G

G

T

T

A
|
A

G
|
G

I
x
V

P
|
P

G

G

A

A

G
|
G

L

A

I

I

M

M

L

L

G

A

L

M

V

V

L

L

T

E

A

S

A

V

G

G

L

L

P

P

L

L

E

T

-

D

-

P

-

N

-

V

-

A

G

A

V

Y

A

A

L

M

I

I

A

L

G

G

I

I

D

D

R

A

I

I

L

L

D
|
D

M

M

A

G

R
x
C

T
|
T

T

M

V

V

N
|
N

V

V

A

T

G

G

D

D

L

L

M

T

T

G

T

T

T

A

L

I

V

V

G

A

R

K

S

T

E

E

binding cysteine: R268 (≠ S270), S270 (= S272), M303 (= M306), T306 (= T309), A345 (= A348), G346 (= G349), V347 (≠ I350), P348 (= P351), G351 (= G354), D386 (= D383), C389 (≠ R386), T390 (= T387), N393 (= N390)

6bavA Crystal structure of gltph r397c in complex with s-benzyl-l-cysteine (see paper)
47% identity, 93% coverage: 16:405/419 of query aligns to 1:408/409 of 6bavA

query
sites
6bavA

R

E

L

Y

P

P

L

V

W

L

Q

Q

Q

K

I

I

L

L

I

I

G

G

L

L

A

I

L

L

G

G

V

A

A

I

A

V

G

G

M

L

A

I

F

L

G

G

-

H

-

Y

-

G

-

Y

A

A

D

D

A

A

-

V

-

K

Q

T

L

Y

L

V

A

K

P

P

I

F

G

G

T

D

L

L

F

F

L

V

N

R

A

L

I

L

K

K

M

M

L

L

I

V

V

M

P

P

L

I

V

V

F

F

V

A

S

S

L

L

V

V

A

V

G

G

I

A

T

A

S

S

M

I

Q

-

D

S

S

P

A

A

K

R

L

L

G

G

R

R

I

V

S

G

L

V

K

K

T

I

I

V

A

V

I

Y

Y

Y

L

L

V

L

T

T

T

S

A

A

F

F

A

A

V

V

S

T

I

L

G

G

L

I

F

M

G

A

A

R

L

L

F

F

S

N

P

P

G

G

E

A

G

G

M

I

N

H

M

L

V

A

A

V

S

G

G

G

N

Q

E

Q

-

F

Q

Q

A

P

K

K

Q

Q

A

A

P

P

S

P

L

L

V

V

S

K

I

I

L

L

V

L

G

D

L

I

V

V

P

P

A

T

N

N

P

P

V

F

T

G

A

A

F

L

A

A

E

N

G

G

N

Q

I

V

L

L

Q

P

I

T

I

I

V

F

F

F

A

A

I

I

A

I

L

L

G

G

V

I

S

A

I

I

N

T

L

Y

I

L

G

M

E

N

R

S

G

E

A

N

P

E

A

K

V

V

R

R

-

K

-

S

-

A

-

E

-

T

L

L

F

L

D

D

A

A

-

I

-

N

-

G

L

L

A

A

E

E

T

A

F

M

Y

Y

K

K

L

I

T

V

D

N

L

G

V

V

M

M

R

Q

V

Y

A

A

P

P

I

I

G

G

V

V

F

F

A

A

L

L

T

I

A

A

G

Y

V

V

V

M

G

A

S

E

H

Q

G

G

A

V

E

K

V

V

L

V

L

G

P

E

L

L

A

A

G

K

V

V

I

T

G

A

V

A

I

V

Y

Y

L

V

A

G

S

L

I

T

A

L

H

Q

V

I

L

L

V

V

Y

Y

G

F

G

V

L

L

G

K

L

I

L

Y

A

G

R

-

L

I

N

D

P

P

L

I

R

S

F

F

F

I

Q

K

G

K

I

A

A

K

P

D

A

A

L

M

A

L

V

T

A

A

F

F

S

V

T

T

S
x
R

S
|
S

S

S

G

G

T

T

L

L

P

P

V

V

S

T

I

M

E

R

C

V

A

A

R

-

K

K

N

E

L

M

G

G

V

I

S

S

E

E

G

G

V

I

A

Y

G

S

F

F

V

T

L

L

P

P

V

L

G

G

A

A

T

T

I

I

N

N

M
|
M

D

D

G

G

T
|
T

A

A

I

L

Y
|
Y

Q

Q

G

G

V

V

L

C

A

T

L

F

F

F

I

I

A

A

Q

N

A

A

F

L

G

G

I

S

D

H

L

L

S

T

A

V

G

G

Q

Q

Y

Q

A

L

M

T

I

I

I

V

L

L

T

T

A

A

T

V

L

L

A

A

S

S

I

I

G

G

T

T

A

A

G

G

I

V

P

P

G

G

A

A

G

G

L

A

I

I

M

M

L

L

G

A

L

M

V

V

L

L

T

E

A

S

A

V

G

G

L

L

P

P

L

L

E

T

-

D

-

P

-

N

-

V

-

A

G

A

V

Y

A

A

L

M

I

I

A

L

G

G

I

I

D
|
D

R

A

I

I

L

L

D
|
D

M

M

A

G

R
x
C

T
|
T

T

M

V

V

N
|
N

V

V

A

T

G

G

D

D

L

L

M

T

T

G

T

T

T

A

L

I

V

V

G

A

R

K

S

T

E

E

binding benzylcysteine: R268 (≠ S270), S269 (= S271), S270 (= S272), M303 (= M306), T306 (= T309), Y309 (= Y312), D382 (= D379), D386 (= D383), C389 (≠ R386), T390 (= T387), N393 (= N390)

2nwwA Crystal structure of gltph in complex with tboa (see paper)
47% identity, 93% coverage: 18:405/419 of query aligns to 2:407/407 of 2nwwA

query
sites
2nwwA

P

P

L

V

W

L

Q

Q

Q

K

I

I

L

L

I

I

G

G

L

L

A

I

L

L

G

G

V

A

A

I

A

V

G

G

M

L

A

I

F

L

G

G

-

H

-

Y

-

G

-

Y

-

A

-

H

A

A

D

V

A

H

Q

T

L

Y

L

V

A

K

P

P

I

F

G

G

T

D

L

L

F

F

L

V

N

R

A

L

I

L

K

K

M

M

L

L

I

V

V

M

P

P

L

I

V

V

F

F

V

A

S

S

L

L

V

V

A

V

G

G

I

A

T

A

S

S

M

I

Q

-

D

S

S

P

A

A

K

R

L

L

G

G

R

R

I

V

S

G

L

V

K

K

T

I

I

V

A

V

I

Y

Y

Y

L

L

V

L

T

T

T

S

A

A

F

F

A

A

V

V

S

T

I

L

G

G

L

I

F

M

G

A

A

R

L

L

F

F

S

N

P

P

G

G

E

A

G

G

M

I

N

H

M

L

V

A

A

V

S

G

G

G

N

Q

E

Q

-

F

Q

Q

A

P

K

H

Q

Q

A

A

P

P

S

P

L

L

V

V

S

H

I

I

L

L

V

L

G

D

L

I

V

V

P

P

A

T

N

N

P

P

V

F

T

G

A

A

F

L

A

A

E

N

G

G

N

Q

I

V

L

L

Q

P

I

T

I

I

V

F

F

F

A

A

I

I

A

I

L

L

G

G

V

I

S

A

I

I

N

T

L

Y

I

L

G

M

E

N

R

S

G

E

A

N

P

E

A

K

V

V

R

R

-

K

-

S

-

A

-

E

-

T

L

L

F

L

D

D

A

A

-

I

-

N

-

G

L

L

A

A

E

E

T

A

F

M

Y

Y

K

K

L

I

T

V

D

N

L

G

V

V

M

M

R

Q

V

Y

A

A

P

P

I

I

G

G

V

V

F

F

A

A

L

L

T

I

A

A

G

Y

V

V

V

M

G

A

S

E

H

Q

G

G

A

V

E

H

V

V

L

V

L

G

P

E

L

L

A

A

G

K

V

V

I

T

G

A

V

A

I

V

Y

Y

L

V

A

G

S

L

I

T

A

L

H

Q

V

I

L

L

V

V

Y

Y

G

F

G

V

L

L

G

K

L

I

L

Y

A

G

R

-

L

I

N

D

P

P

L

I

R

S

F

F

F

I

Q

K

G

H

I

A

A

K

P

D

A

A

L

M

A

L

V

T

A

A

F

F

S

V

T

T

S
x
R

S
|
S

S

S

G

G

T

T

L

L

P

P

V

V

S

T

I

M

E

R

C

V

A

A

R

-

K

K

N

E

L

M

G

G

V

I

S

S

E

E

G

G

V

I

A

Y

G

S

F

F

V

T

L

L

P

P

V

L

G

G

A

A

T

T

I

I

N

N

M

M

D

D

G

G

T
|
T

A

A

I

L

Y

Y

Q

Q

G

G

V

V

L

C

A

T

L

F

F

F

I

I

A

A

Q

N

A

A

F

L

G

G

I

S

D

H

L

L

S

T

A

V

G

G

Q

Q

Y

Q

A

L

M

T

I

I

I

V

L

L

T

T

A

A

T

V

L

L

A

A

S

S

I

I

G

G

T

T

A

A

G

G

I

V

P

P

G
|
G

A
|
A

G
|
G

L

A

I

I

M

M

L

L

G

A

L

M

V

V

L

L

T

H

A

S

A

V

G

G

L

L

P

P

L

L

E

T

-

D

-

P

-

N

-

V

-

A

G

A

V

Y

A

A

L

M

I

I

A

L

G

G

I

I

D

D

R

A

I

I

L

L

D
|
D

M

M

A

G

R
|
R

T
|
T

T

M

V

V

N
|
N

V

V

A

T

G

G

D

D

L

L

M

T

T

G

T

T

T

A

L

I

V

V

G

A

R

K

S

T

E

E

binding (3s)-3-(benzyloxy)-l-aspartic acid: R267 (≠ S270), S268 (= S271), S269 (= S272), T305 (= T309), G348 (= G352), A349 (= A353), G350 (= G354), D385 (= D383), R388 (= R386), T389 (= T387), N392 (= N390)

6x15A Inward-facing state of the glutamate transporter homologue gltph in complex with l-aspartate and sodium ions (see paper)
46% identity, 95% coverage: 12:408/419 of query aligns to 5:419/419 of 6x15A

query
sites
6x15A

R

R

L
x
K

L
x
Y

V
x
I

R

E

L

Y

P

P

L

V

W

L

Q

Q

Q
x
K

I

I

L

L

I
|
I

G

G

L

L

A

I

L

L

G

G

V

A

A

I

A

V

G

G

M

L

A

I

F

L

G

G

-

H

-

Y

-

G

-

Y

-

A

-

H

A

A

D

V

A

H

Q

T

L
x
Y

L

V

A

K

P
|
P

I
x
F

G

G

T

D

L
|
L

F

F

L

V

N

R

A
x
L

I

L

K

C

M

M

L

L

I

V

V

M

P

P

L

I

V

V

F

F

V

A

S

S

L

L

V

V

A

V

G

G

I

A

T

A

S

S

M
x
I

Q

-

D

S

S
x
P

A

A

K

R

L

L

G

G

R

R

I

V

S

G

L

V

K
|
K

T

I

I

V

A

V

I

Y

Y
|
Y

L
|
L

V
x
L

T
|
T

T
x
S

A

A

F
|
F

A

A

V

V

S

T

I

L

G

G

L

I

F

M

G

A

A

R

L

L

F

F

S

N

P

P

G

G

E

A

G

G

M

I

N

H

M

L

V

A

A

V

S

G

G

G

N

Q

E

Q

-

F

Q

Q

A

P

K

H

Q

Q

A

A

P

P

S

P

L
|
L

V
|
V

S

H

I
|
I

L
|
L

V

L

G

D

L
x
I

V

V

P

P

A

T

N

N

P

P

V

F

T

G

A

A

F

L

A

A

E

N

G

G

N

Q

I

V

L
|
L

Q

P

I

T

I

I

V
x
F

F

F

A

A

I
|
I

A

I

L

L

G

G

V
x
I

S
x
A

I

I

N

T

L
x
Y

I

L

G

M

E

N

R

S

G

E

A

N

P

E

A

K

V

V

R

R

-

K

-

S

-

A

-

E

-

T

L

L

F

L

D

D

A

A

-

I

-

N

-

G

L

L

A

A

E

E

T

A

F

M

Y

Y

K
|
K

L
x
I

T

V

D
x
N

L
x
G

V

V

M

M

R

Q

V
x
Y

A

A

P

P

I
|
I

G

G

V

V

F
|
F

A

A

L

L

T

I

A

A

G

Y

V

V

V

M

G

A

S

E

H

Q

G

G

A

V

E

H

V

V

L

V

L

G

P

E

L

L

A

A

G

K

V

V

I

T

G

A

V

A

I

V

Y

Y

L

V

A

G

S

L

I

T

A

L

H

Q

V

I

L

L

V

V

Y

Y

G

F

G

V

L
|
L

L

L

G

K

L
x
I

L
x
Y

A

G

R

-

L

I

N

D

P

P

L

I

R

S

F

F

F
x
I

Q

K

G

H

I

A

A
x
K

P

D

A

A

L
x
M

A

L

V

T

A

A

F

F

S

V

T

T

S
x
R

S
|
S

S

S

G

G

T

T

L

L

P

P

V

V

S

T

I

M

E

R

C

V

A

A

R

-

K

K

N

E

L

M

G

G

V

I

S

S

E

E

G

G

V

I

A

Y

G

S

F

F

V

T

L

L

P

P

V

L

G
|
G

A
|
A

T
|
T

I

I

N
|
N

M
|
M

D
|
D

G

G

T
|
T

A

A

I

L

Y

Y

Q

Q

G

G

V

V

L

A

A

T

L

F

F

F

I

I

A

A

Q

N

A

A

F

L

G

G

I

S

D

H

L

L

S
x
T

A
x
V

G
|
G

Q
|
Q

Y

Q

A
x
L

M
x
T

I

I

I

V

L
|
L

T

T

A

A

T

V

L

L

A

A

S
|
S

I
|
I

G
|
G

T
|
T

A

A

G
|
G

I
x
V

P

P

G
|
G

A
|
A

G
|
G

L

A

I

I

M

M

L

L

G

C

L

M

V

V

L

L

T

H

A
x
S

A

V

G

G

L

L

P

P

L

L

E

T

-

D

-

P

-

N

-

V

-

A

G

A

V

Y

A

A

L

M

I

I

A

L

G

G

I

I

D

D

R

A

I

I

L

L

D
|
D

M

M

A

G

R
|
R

T
|
T

T
x
M

V

V

N
|
N

V
|
V

A

T

G

G

D
|
D

L

L

M

T

T

G

T

T

T

A

L

I

V

V

G

A

R

K

S

T

E

E

Q

G

E

T

L

L

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: K6 (≠ L13), Y7 (≠ L14), I8 (≠ V15), K15 (≠ Q22), I18 (= I25), Y42 (≠ L43), P45 (= P46), F46 (≠ I47), F46 (≠ I47), F46 (≠ I47), L49 (= L50), L49 (= L50), L53 (≠ A54), I73 (≠ M74), P75 (≠ S77), K84 (= K86), L90 (= L92), L91 (≠ V93), L91 (≠ V93), F95 (= F97), L130 (= L131), V131 (= V132), I133 (= I134), L134 (= L135), I137 (≠ L138), L152 (= L153), F156 (≠ V157), I159 (= I160), I163 (≠ V164), I163 (≠ V164), I163 (≠ V164), A164 (≠ S165), Y167 (≠ L168), Y167 (≠ L168), K196 (= K189), I197 (≠ L190), N199 (≠ D192), G200 (≠ L193), Y204 (≠ V197), I207 (= I200), F210 (= F203), L250 (= L243), I253 (≠ L246), Y254 (≠ L247), Y254 (≠ L247), I262 (≠ F256), K266 (≠ A260), M269 (≠ L263), T334 (≠ S329), V335 (≠ A330), G336 (= G331), Q337 (= Q332), L339 (≠ A334), T340 (≠ M335), L343 (= L338), S369 (≠ A364), M399 (≠ T388)
binding aspartic acid: R276 (≠ S270), S277 (= S271), S278 (= S272), T314 (= T309), T352 (= T347), G354 (= G349), V355 (≠ I350), G357 (= G352), A358 (= A353), G359 (= G354), D394 (= D383), R397 (= R386), T398 (= T387), N401 (= N390)
binding sodium ion: Y89 (= Y91), T92 (= T94), S93 (≠ T95), G306 (= G301), A307 (= A302), T308 (= T303), N310 (= N305), N310 (= N305), M311 (= M306), D312 (= D307), S349 (= S344), I350 (= I345), G351 (= G346), T352 (= T347), N401 (= N390), V402 (= V391), D405 (= D394)

Sites not aligning to the query:

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: 1, 3, 4, 4

6x14A Inward-facing state of the glutamate transporter homologue gltph in complex with tfb-tboa (see paper)
47% identity, 94% coverage: 12:405/419 of query aligns to 2:413/413 of 6x14A

query
sites
6x14A

R

R

L

K

L
x
Y

V

I

R

E

L

Y

P

P

L

V

W

L

Q

Q

Q

K

I
|
I

L

L

I

I

G

G

L

L

A

I

L

L

G

G

V

A

A

I

A

V

G

G

M

L

A

I

F

L

G

G

-

H

-

Y

-

G

-

Y

-

A

-

H

A

A

D

V

A

H

Q

T

L

Y

L

V

A

K

P

P

I

F

G

G

T

D

L

L

F
|
F

L

V

N

R

A

L

I

L

K

C

M

M

L

L

I

V

V

M

P

P

L

I

V

V

F

F

V

A

S

S

L

L

V

V

A

V

G
|
G

I
x
A

T

A

S

S

M
x
I

Q

-

D

S

S

P

A

A

K

R

L

L

G

G

R

R

I

V

S

G

L

V

K

K

T

I

I
x
V

A

V

I

Y

Y

Y

L

L

V

L

T

T

T

S

A

A

F

F

A

A

V

V

S

T

I

L

G

G

L

I

F

M

G

A

A

R

L

L

F

F

S

N

P

P

G

G

E

A

G

G

M

I

N

H

M

L

V

A

A

V

S

G

G

G

N

Q

E

Q

-

F

Q

Q

A

P

K

H

Q

Q

A

A

P

P

S

P

L

L

V

V

S

H

I

I

L

L

V

L

G

D

L

I

V

V

P

P

A

T

N

N

P

P

V

F

T

G

A

A

F

L

A

A

E

N

G

G

N

Q

I

V

L

L

Q

P

I

T

I

I

V

F

F

F

A

A

I

I

A
x
I

L

L

G

G

V

I

S

A

I

I

N

T

L
x
Y

I

L

G

M

E

N

R

S

G

E

A

N

P

E

A

K

V

V

R

R

-

K

-

S

-

A

-

E

-

T

L

L

F

L

D

D

A

A

-

I

-

N

-

G

L

L

A

A

E

E

T

A

F

M

Y

Y

K
|
K

L
x
I

T

V

D
x
N

L
x
G

V

V

M
|
M

R
x
Q

V
x
Y

A

A

P

P

I

I

G

G

V

V

F

F

A

A

L

L

T

I

A

A

G

Y

V

V

V

M

G

A

S

E

H

Q

G

G

A

V

E

H

V

V

L

V

L

G

P

E

L

L

A

A

G

K

V

V

I

T

G

A

V

A

I

V

Y

Y

L

V

A

G

S

L

I

T

A

L

H

Q

V

I

L

L

V

V

Y

Y

G

F

G

V

L

L

G

K

L

I

L

Y

A

G

R

-

L

I

N

D

P

P

L

I

R

S

F

F

F

I

Q

K

G

H

I

A

A

K

P

D

A

A

L

M

A

L

V

T

A

A

F

F

S

V

T

T

S
x
R

S
|
S

S

S

G

G

T

T

L

L

P

P

V

V

S

T

I

M

E

R

C

V

A

A

R

-

K

K

N

E

L

M

G

G

V

I

S

S

E

E

G

G

V

I

A

Y

G

S

F

F

V

T

L

L

P

P

V
x
L

G

G

A

A

T
|
T

I
|
I

N

N

M
|
M

D

D

G

G

T
|
T

A

A

I

L

Y

Y

Q

Q

G

G

V

V

L

A

A

T

L

F

F

F

I

I

A

A

Q

N

A

A

F

L

G

G

I

S

D

H

L

L

S

T

A

V

G

G

Q

Q

Y

Q

A

L

M

T

I

I

I

V

L

L

T

T

A

A

T

V

L

L

A

A

S

S

I
|
I

G

G

T

T

A
|
A

G

G

I

V

P
|
P

G

G

A

A

G
|
G

L
x
A

I

I

M

M

L

L

G

C

L

M

V

V

L

L

T

H

A

S

A

V

G

G

L

L

P

P

L

L

E

T

-

D

-

P

-

N

-

V

-

A

G

A

V

Y

A

A

L

M

I

I

A
x
L

G

G

I

I

D

D

R

A

I

I

L

L

D
|
D

M

M

A

G

R
|
R

T
|
T

T

M

V

V

N

N

V

V

A

T

G

G

D

D

L

L

M

T

T

G

T

T

T

A

L

I

V

V

G

A

R

K

S

T

E

E

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: Y4 (≠ L14), G66 (= G70), A67 (≠ I71), I70 (≠ M74), V83 (≠ I88), I157 (≠ A161), Y164 (≠ L168), K193 (= K189), I194 (≠ L190), N196 (≠ D192), G197 (≠ L193), Q200 (≠ R196), Y201 (≠ V197), L302 (≠ V300), T305 (= T303), I306 (= I304), I347 (= I345), A350 (= A348)
binding (2~{S},3~{S})-2-azanyl-3-[[3-[[4-(trifluoromethyl)phenyl]carbonylamino]phenyl]methoxy]butanedioic acid: I13 (= I23), F47 (= F51), M199 (= M195), R273 (≠ S270), S274 (= S271), S275 (= S272), M308 (= M306), T311 (= T309), P353 (= P351), G356 (= G354), A357 (≠ L355), L384 (≠ A376), D391 (= D383), R394 (= R386), T395 (= T387)

Sites not aligning to the query:

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: 1

5e9sA Crystal structure of substrate-bound glutamate transporter homologue glttk (see paper)
44% identity, 97% coverage: 11:416/419 of query aligns to 2:427/427 of 5e9sA

query
sites
5e9sA

L

L

R

R

L

R

L

Y

V

L

R

D

L

Y

P

P

L

V

W

L

Q

W

Q

K

I

I

L

L

I

W

G

G

L

L

A

V

L

L

G

G

V

A

A

V

A

F

G

G

M

L

-

I

-

A

-

G

-

H

-

F

-

G

A

Y

F

A

G

G

A

A

D

V

A

K

Q

T

L

Y

L

I

A

K

P

P

I

F

G

G

T

D

L

L

F

F

L

V

N

R

A

L

I

L

K

K

M

M

L

L

I

V

V

M

P

P

L

I

V

V

F

L

V

A

S

S

L

L

V

V

A

V

G

G

I

A

T

A

S

S

M

I

Q

-

D

S

S

P

A

A

K

R

L

L

G

G

R

R

I

V

S

G

L

V

K

K

T

I

I

V

A

V

I

Y

Y
|
Y

L

L

V

A

T
|
T

T
x
S

A

A

F

M

A

A

V

V

S

F

I

F

G

G

L

L

L

I

F

V

G

G

A

R

L

L

F

F

S

N

P

V

G

G

E

A

G

N

M

V

N

N

M

L

-

G

V

S

A

G

S

T

G

G

N

K

E

A

Q

I

A

E

K

A

Q

Q

A

P

P

P

S

S

L

L

V

V

S

Q

I

T

L

L

V

L

G

N

L

I

V

V

P

P

A

T

N

N

P

P

V

F

T

A

A

S

F

L

A

A

E

K

G

G

N

E

I

V

L

L

Q

P

I

V

I

I

V

F

F

F

A

A

I

I

A

I

L

L

G

G

V

I

S

A

I

I

N

T

L

Y

I

L

G

M

E

N

R

R

G

N

A

E

P

E

A

R

V

V

R

R

-

K

-

S

-

A

-

E

-

T

-

L

-

R

L

V

F

F

D

D

A

G

L

L

A

A

E

E

T

A

F

M

Y

Y

K
x
L

L

I

T

V

D
x
G

L
x
G

V

V

M

M

R

Q

V
x
Y

A

A

P

P

I

I

G

G

V

V

F

F

A

A

L

L

T

I

A

A

G

Y

V

V

V

M

G

A

S

E

H

Q

G

G

A

V

E

R

V

V

L

V

L

G

P

P

L

L

A

A

G

K

V

V

I

V

G

G

V

A

I

V

Y

Y

L

T

A

G

S

L

I

F

A

L

H

Q

V

I

L

V

L

I

V

T

Y

Y

G

F

G

I

L

L

G

K

L

V

L

F

A

G

R

-

L

I

N

D

P

P

L

I

R

K

F

F

F

I

Q

R

G

K

I

A

A

K

P

D

A

A

L

M

A

I

V

T

A

A

F

F

S

V

T

T

S
x
R

S
|
S

S

S

G

G

T

T

L

L

P

P

V

V

S

T

I

M

E
x
R

C

V

A

A

R

E

K

E

N

E

L

M

G

G

V

V

S

D

E

K

G

G

V

I

A

F

G

S

F

F

V

T

L

L

P

P

V

L

G
|
G

A
|
A

T
|
T

I
|
I

N
|
N

M
|
M

D
|
D

G

G

T
|
T

A

A

I

L

Y

Y

Q

Q

G

G

V

V

L

T

A

V

L

L

F

F

I

V

A

A

Q

N

A

A

F

I

G

G

I

H

D

P

L

L

S

T

A

L

G

G

Q

Q

Y

Q

A

L

M

V

I

V

L

L

T

T

A

A

T

V

L

L

A

A

S
|
S

I
|
I

G
|
G

T
|
T

A
|
A

G
|
G

I
x
V

P
|
P

G
|
G

A
|
A

G
|
G

L

A

I

I

M

M

L

L

G

A

L

M

V

V

L

L

T

Q

A

S

A

V

G

G

L

L

P

D

L

L

E

T

-

P

-

G

-

S

-

P

-

V

-

A

-

L

G

A

V

Y

A

A

L

M

I

I

A

L

G

G

I

I

D

D

R

A

I

I

L

L

D
|
D

M

M

A

G

R
|
R

T
|
T

T

M

V

V

N
|
N

V
|
V

A

T

G

G

D
|
D

L

L

M

A

T

G

T

T

T

V

L

I

V

V

G

A

R

K

S

T

E

E

Q

K

E

E

L

L

D

D

R

E

A

S

I

K

Y

W

D

I

S

S

S

H

binding aspartic acid: R274 (≠ S270), S275 (= S271), S276 (= S272), T313 (= T309), T351 (= T347), A352 (= A348), G353 (= G349), V354 (≠ I350), P355 (= P351), G356 (= G352), A357 (= A353), G358 (= G354), D394 (= D383), R397 (= R386), T398 (= T387), N401 (= N390)
binding decyl-beta-d-maltopyranoside: L194 (≠ K189), G197 (≠ D192), G198 (≠ L193), Y202 (≠ V197), R285 (≠ E281)
binding sodium ion: Y87 (= Y91), T90 (= T94), S91 (≠ T95), S276 (= S272), G305 (= G301), A306 (= A302), T307 (= T303), I308 (= I304), N309 (= N305), N309 (= N305), M310 (= M306), M310 (= M306), D311 (= D307), S348 (= S344), I349 (= I345), G350 (= G346), T351 (= T347), N401 (= N390), V402 (= V391), D405 (= D394)

6xwnB Structure of glutamate transporter homologue glttk in the presence of tboa inhibitor (see paper)
44% identity, 97% coverage: 11:415/419 of query aligns to 2:426/426 of 6xwnB

query
sites
6xwnB

L

L

R

R

L

R

L

Y

V

L

R

D

L

Y

P

P

L

V

W

L

Q

W

Q

K

I

I

L

L

I

W

G

G

L

L

A

V

L

L

G

G

V

A

A

V

A

F

G

G

M

L

-

I

-

A

-

G

-

H

-

F

-

G

A

Y

F

A

G

G

A

A

D

V

A

K

Q

T

L

Y

L

I

A

K

P

P

I

F

G

G

T

D

L

L

F

F

L

V

N

R

A

L

I

L

K

K

M

M

L

L

I

V

V

M

P

P

L

I

V

V

F

L

V

A

S

S

L

L

V

V

A

V

G

G

I

A

T

A

S

S

M

I

Q

-

D

S

S

P

A

A

K

R

L

L

G

G

R

R

I

V

S

G

L

V

K

K

T

I

I

V

A

V

I

Y

Y

Y

L

L

V

A

T

T

T

S

A

A

F

M

A

A

V

V

S

F

I

F

G

G

L

L

L

I

F

V

G

G

A

R

L

L

F

F

S

N

P

V

G

G

E

A

G

N

M

V

N

N

M

L

-

G

V

S

A

G

S

T

G

G

N

K

E

A

Q

I

A

E

K

A

Q

Q

A

P

P

P

S

S

L

L

V

V

S

Q

I

T

L

L

V

L

G

N

L

I

V

V

P

P

A

T

N

N

P

P

V

F

T

A

A

S

F

L

A

A

E

K

G

G

N

E

I

V

L

L

Q

P

I

V

I

I

V

F

F

F

A

A

I

I

A

I

L

L

G

G

V

I

S

A

I

I

N

T

L

Y

I

L

G

M

E

N

R

R

G

N

A

E

P

E

A

R

V

V

R

R

-

K

-

S

-

A

-

E

-

T

-

L

-

R

L

V

F

F

D

D

A

G

L

L

A

A

E

E

T

A

F

M

Y

Y

K

L

L

I

T

V

D

G

L

G

V

V

M

M

R

Q

V

Y

A

A

P

P

I

I

G

G

V

V

F

F

A

A

L

L

T

I

A

A

G

Y

V

V

V

M

G

A

S

E

H

Q

G

G

A

V

E

R

V

V

L

V

L

G

P

P

L

L

A

A

G

K

V

V

I

V

G

G

V

A

I

V

Y

Y

L

T

A

G

S

L

I

F

A

L

H

Q

V

I

L

V

L

I

V

T

Y

Y

G

F

G

I

L

L

G

K

L

V

L

F

A

G

R

-

L

I

N

D

P

P

L

I

R

K

F

F

F

I

Q

R

G

K

I

A

A

K

P

D

A

A

L

M

A

I

V

T

A

A

F

F

S

V

T

T

S
x
R

S
|
S

S

S

G

G

T

T

L

L

P

P

V

V

S

T

I

M

E

R

C

V

A

A

R

E

K

E

N

E

L

M

G

G

V

V

S

D

E

K

G

G

V

I

A

F

G

S

F

F

V

T

L

L

P

P

V

L

G

G

A

A

T
|
T

I

I

N

N

M
|
M

D

D

G

G

T
|
T

A

A

I

L

Y

Y

Q

Q

G

G

V

V

L

T

A

V

L

L

F

F

I

V

A

A

Q

N

A

A

F

I

G

G

I

H

D

P

L

L

S

T

A

L

G

G

Q

Q

Y

Q

A

L

M

V

I

V

L

L

T

T

A

A

T

V

L

L

A

A

S

S

I

I

G

G

T

T

A

A

G

G

I

V

P

P

G

G

A

A

G
|
G

L

A

I

I

M

M

L

L

G

A

L

M

V

V

L

L

T

Q

A

S

A

V

G

G

L

L

P

D

L

L

E

T

-

P

-

G

-

S

-

P

-

V

-

A

-

L

G

A

V

Y

A

A

L

M

I

I

A

L

G

G

I

I

D

D

R

A

I

I

L

L

D
|
D

M

M

A

G

R
|
R

T
|
T

T

M

V

V

N

N

V

V

A

T

G

G

D

D

L

L

M

A

T

G

T

T

T

V

L

I

V

V

G

A

R

K

S

T

E

E

Q

K

E

E

L

L

D

D

R

E

A

S

I

K

Y

W

D

I

S

S

binding (3s)-3-(benzyloxy)-l-aspartic acid: R274 (≠ S270), S275 (= S271), S276 (= S272), T307 (= T303), M310 (= M306), T313 (= T309), G358 (= G354), D394 (= D383), R397 (= R386), T398 (= T387)

6zgbA Glutamate transporter homologue glttk in complex with a photo cage compound (see paper)
44% identity, 97% coverage: 12:416/419 of query aligns to 1:425/425 of 6zgbA

query
sites
6zgbA

R

R

L

R

L

Y

V

L

R

D

L

Y

P

P

L

V

W

L

Q

W

Q

K

I

I

L

L

I

W

G

G

L

L

A

V

L

L

G

G

V

A

A

V

A

F

G

G

M

L

-

I

-

A

-

G

-

H

-

F

-

G

A

Y

F

A

G

G

A

A

D

V

A

K

Q

T

L

Y

L

I

A

K

P

P

I

F

G

G

T

D

L

L

F

F

L

V

N

R

A

L

I

L

K

K

M

M

L

L

I

V

V

M

P

P

L

I

V

V

F

L

V

A

S

S

L

L

V

V

A

V

G

G

I

A

T

A

S

S

M

I

Q

-

D

S

S

P

A

A

K

R

L

L

G

G

R

R

I

V

S

G

L

V

K

K

T

I

I

V

A

V

I

Y

Y

Y

L

L

V

A

T

T

T

S

A

A

F

M

A

A

V

V

S

F

I

F

G

G

L

L

L

I

F

V

G

G

A

R

L

L

F

F

S

N

P

V

G

G

E

A

G

N

M

V

N

N

M

L

-

G

V

S

A

G

S

T

G

G

N

K

E

A

Q

I

A

E

K

A

Q

Q

A

P

P

P

S

S

L

L

V

V

S

Q

I

T

L

L

V

L

G

N

L

I

V

V

P

P

A

T

N

N

P

P

V

F

T

A

A

S

F

L

A

A

E

K

G

G

N

E

I

V

L

L

Q

P

I

V

I

I

V

F

F

F

A

A

I

I

A

I

L

L

G

G

V

I

S

A

I

I

N

T

L

Y

I

L

G

M

E

N

R

R

G

N

A

E

P

E

A

R

V

V

R

R

-

K

-

S

-

A

-

E

-

T

-

L

-

R

L

V

F

F

D

D

A

G

L

L

A

A

E

E

T

A

F

M

Y

Y

K

L

L

I

T

V

D

G

L

G

V

V

M

M

R

Q

V

Y

A

A

P

P

I

I

G

G

V

V

F

F

A

A

L

L

T

I

A

A

G

Y

V

V

V

M

G

A

S

E

H

Q

G

G

A

V

E

R

V

V

L

V

L

G

P

P

L

L

A

A

G

K

V

V

I

V

G

G

V

A

I

V

Y

Y

L

T

A

G

S

L

I

F

A

L

H

Q

V

I

L

V

L

I

V

T

Y

Y

G

F

G

I

L

L

G

K

L

V

L

F

A

G

R

-

L

I

N

D

P

P

L

I

R

K

F

F

F

I

Q

R

G

K

I

A

A

K

P

D

A

A

L

M

A

I

V

T

A

A

F

F

S

V

T

T

S
x
R

S
|
S

S

S

G

G

T

T

L

L

P

P

V

V

S

T

I

M

E

R

C

V

A

A

R

E

K

E

N

E

L

M

G

G

V

V

S

D

E

K

G

G

V

I

A

F

G

S

F

F

V

T

L

L

P

P

V

L

G

G

A

A

T

T

I

I

N

N

M
|
M

D

D

G

G

T
|
T

A

A

I

L

Y

Y

Q

Q

G

G

V

V

L

T

A

V

L

L

F

F

I

V

A

A

Q

N

A

A

F

I

G

G

I

H

D

P

L

L

S

T

A

L

G

G

Q

Q

Y

Q

A

L

M

V

I

V

L

L

T

T

A

A

T

V

L

L

A

A

S

S

I

I

G

G

T

T

A

A

G

G

I

V

P

P

G

G

A

A

G

G

L

A

I

I

M

M

L

L

G

A

L

M

V

V

L

L

T

Q

A

S

A

V

G

G

L

L

P

D

L

L

E

T

-

P

-

G

-

S

-

P

-

V

-

A

-

L

G

A

V

Y

A

A

L

M

I

I

A

L

G

G

I

I

D

D

R

A

I

I

L

L

D
|
D

M

M

A

G

R
|
R

T
|
T

T

M

V

V

N

N

V

V

A

T

G

G

D

D

L

L

M

A

T

G

T

T

T

V

L

I

V

V

G

A

R

K

S

T

E

E

Q

K

E

E

L

L

D

D

R

E

A

S

I

K

Y

W

D

I

S

S

S

H

binding (2~{S},3~{S})-2-azanyl-3-[(2-nitrophenyl)methoxy]butanedioic acid: R272 (≠ S270), S273 (= S271), S274 (= S272), M308 (= M306), T311 (= T309), D392 (= D383), R395 (= R386), T396 (= T387)

6zl4A The structure of glutamate transporter homologue glttk in complex with the photo switchable compound (cis) (see paper)
44% identity, 95% coverage: 18:416/419 of query aligns to 6:424/424 of 6zl4A

query
sites
6zl4A

P

P

L

V

W

L

Q

W

Q

K

I

I

L

L

I

W

G

G

L

L

A

V

L

L

G

G

V

A

A

V

A

F

G

G

M

L

-

I

-

A

-

G

-

H

-

F

-

G

A

Y

F

A

G

G

A

A

D

V

A

K

Q

T

L

Y

L

I

A

K

P

P

I

F

G

G

T

D

L

L

F

F

L

V

N

R

A

L

I

L

K

K

M

M

L

L

I

V

V

M

P

P

L

I

V

V

F

L

V

A

S

S

L

L

V

V

A

V

G

G

I

A

T

A

S

S

M

I

Q

S

D

-

S

P

A

A

K

R

L

L

G

G

R

R

I

V

S

G

L

V

K

K

T

I

I

V

A

V

I

Y

Y

Y

L

L

V

A

T

T

T

S

A

A

F

M

A

A

V

V

S

F

I

F

G

G

L

L

L

I

F

V

G

G

A

R

L

L

F

F

S

N

P

V

G

G

E

A

G

N

M

V

N

N

M

L

-

G

V

S

A

G

S

T

G

G

N

K

E

A

Q

I

A

E

K

A

Q

Q

A

P

P

P

S

S

L

L

V

V

S

Q

I

T

L

L

V

L

G

N

L

I

V

V

P

P

A

T

N

N

P

P

V

F

T

A

A

S

F

L

A

A

E

K

G

G

N

E

I

V

L

L

Q

P

I

V

I

I

V

F

F

F

A

A

I

I

A

I

L

L

G

G

V

I

S

A

I

I

N

T

L

Y

I

L

G

M

E

N

R

R

G

N

A

E

P

E

A

R

V

V

R

R

-

K

-

S

-

A

-

E

-

T

-

L

-

R

L

V

F

F

D

D

A

G

L

L

A

A

E

E

T

A

F

M

Y
|
Y

K
x
L

L

I

T

V

D
x
G

L
x
G

V

V

M

M

R
x
Q

V

Y

A

A

P

P

I

I

G

G

V

V

F

F

A

A

L

L

T

I

A

A

G

Y

V

V

V

M

G

A

S

E

H

Q

G

G

A

V

E

R

V

V

L

V

L

G

P

P

L

L

A

A

G

K

V

V

I

V

G

G

V

A

I

V

Y

Y

L

T

A

G

S

L

I

F

A

L

H

Q

V

I

L

V

L

I

V

T

Y

Y

G

F

G

I

L

L

G

K

L

V

L

F

A

G

R

-

L

I

N

D

P

P

L

I

R

K

F

F

F

I

Q

R

G

K

I

A

A

K

P

D

A

A

L

M

A

I

V

T

A

A

F

F

S

V

T

T

S
x
R

S
|
S

S

S

G

G

T

T

L

L

P

P

V

V

S

T

I

M

E
x
R

C

V

A

A

R

E

K
x
E

N

E

L

M

G

G

V

V

S

D

E

K

G

G

V

I

A

F

G

S

F

F

V

T

L

L

P

P

V

L

G

G

A

A

T

T

I

I

N

N

M
|
M

D

D

G

G

T
|
T

A

A

I

L

Y

Y

Q

Q

G

G

V

V

L

T

A

V

L

L

F

F

I

V

A

A

Q

N

A

A

F

I

G

G

I

H

D

P

L

L

S

T

A

L

G

G

Q

Q

Y

Q

A

L

M

V

I

V

L

L

T

T

A

A

T

V

L

L

A

A

S

S

I

I

G

G

T

T

A

A

G

G

I
x
V

P
|
P

G
|
G

A
|
A

G
|
G

L

A

I

I

M

M

L

L

G

A

L

M

V

V

L

L

T

Q

A

S

A

V

G

G

L

L

P

D

L

L

E

T

-

P

-

G

-

S

-

P

-

V

-

A

-

L

G

A

V

Y

A

A

L

M

I

I

A

L

G

G

I

I

D

D

R

A

I

I

L

L

D
|
D

M

M

A

G

R
|
R

T
|
T

T

M

V

V

N
|
N

V

V

A

T

G

G

D

D

L

L

M

A

T

G

T

T

T

V

L

I

V

V

G

A

R

K

S

T

E

E

Q

K

E

E

L

L

D

D

R

E

A

S

I

K

Y

W

D

I

S

S

S

H

binding decyl-beta-d-maltopyranoside: Y190 (= Y188), L191 (≠ K189), G194 (≠ D192), G195 (≠ L193), Q198 (≠ R196), R282 (≠ E281), E286 (≠ K285)
binding (2~{S},3~{S})-2-azanyl-3-[[4-[2-(4-methoxyphenyl)hydrazinyl]phenyl]methoxy]butanedioic acid: R271 (≠ S270), S272 (= S271), S273 (= S272), M307 (= M306), T310 (= T309), V351 (≠ I350), P352 (= P351), G353 (= G352), A354 (= A353), G355 (= G354), D391 (= D383), R394 (= R386), T395 (= T387), N398 (= N390)

Sites not aligning to the query:

binding decyl-beta-d-maltopyranoside: 3

6r7rA Crystal structure of the glutamate transporter homologue glttk in complex with d-aspartate (see paper)
44% identity, 95% coverage: 18:416/419 of query aligns to 2:416/416 of 6r7rA

query
sites
6r7rA

P

P

L

V

W

L

Q

W

Q

K

I

I

L

L

I

W

G

G

L

L

A

V

L

L

G

G

V

A

A

V

A

F

G

G

M

L

-

I

-

A

-

G

-
x
H

-

F

-

G

A

Y

F

A

G

G

A

A

D

V

A

K

Q

T

L

Y

L

I

A

K

P

P

I

F

G

G

T

D

L

L

F

F

L

V

N

R

A

L

I

L

K

K

M

M

L

L

I

V

V

M

P

P

L

I

V

V

F

L

V

A

S

S

L

L

V

V

A

V

G

G

I

A

T

A

S

S

M

I

Q

-

D

S

S

P

A

A

K

R

L

L

G

G

R

R

I

V

S

G

L

V