SitesBLAST
Comparing GFF402 FitnessBrowser__Phaeo:GFF402 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
I6YCA3 Acyl-CoA dehydrogenase FadE26; ACAD; 3-oxocholest-4-en-26-oyl-CoA dehydrogenase alpha subunit; EC 1.3.99.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
35% identity, 99% coverage: 1:396/399 of query aligns to 1:394/400 of I6YCA3
- IGYS 127:130 (≠ QGYS 126:129) binding
- T136 (≠ S135) binding
- S162 (≠ T161) binding
- E247 (= E244) mutation to A: Loss of dehydrogenase activity.
- TNE 380:382 (≠ SNE 382:384) binding
4x28A Crystal structure of the chse4-chse5 complex from mycobacterium tuberculosis (see paper)
35% identity, 99% coverage: 1:396/399 of query aligns to 1:381/386 of 4x28A
- active site: Y122 (= Y128), S123 (= S129), E240 (= E244), G365 (= G380), M377 (≠ K392)
- binding dihydroflavine-adenine dinucleotide: I120 (≠ Q126), Y122 (= Y128), S123 (= S129), G128 (= G134), T129 (≠ S135), W153 (= W159), S155 (≠ T161), F363 (≠ I378), T367 (≠ S382), E369 (= E384), V370 (≠ I385)
6wy8B Tcur3481-tcur3483 steroid acad (see paper)
34% identity, 99% coverage: 1:396/399 of query aligns to 1:380/384 of 6wy8B
- active site: Y126 (= Y128), T127 (≠ S129), E241 (= E244), T376 (≠ K392)
- binding flavin-adenine dinucleotide: I124 (≠ Q126), Y126 (= Y128), T127 (≠ S129), G132 (= G134), T133 (≠ S135), F157 (≠ W159), S159 (≠ T161), V359 (≠ F379), F362 (vs. gap), G363 (vs. gap), V366 (≠ S382), E368 (= E384)
P71858 Acyl-CoA dehydrogenase FadE29; ACAD; 3-oxo-23,24-bisnorchol-4-en-22-oyl-CoA dehydrogenase beta subunit; 3-oxo-4-pregnene-20-carboxyl-CoA dehydrogenase beta subunit; EC 1.3.99.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
37% identity, 87% coverage: 50:395/399 of query aligns to 48:380/387 of P71858
- E241 (= E244) mutation to Q: Unable to dehydrogenate pregnene-carboxyl-CoA ester.
6wy9A Tcur3481-tcur3483 steroid acad g363a variant (see paper)
34% identity, 96% coverage: 12:396/399 of query aligns to 8:376/380 of 6wy9A
- active site: Y122 (= Y128), T123 (≠ S129), E237 (= E244), T372 (≠ K392)
- binding dihydroflavine-adenine dinucleotide: I120 (≠ Q126), Y122 (= Y128), T123 (≠ S129), G128 (= G134), T129 (≠ S135), F153 (≠ W159), S155 (≠ T161), F358 (vs. gap), V362 (≠ S382), E364 (= E384)
P96855 Acyl-CoA dehydrogenase FadE34; ACAD; 3-oxochol-4-en-24-oyl-CoA dehydrogenase; EC 1.3.99.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
35% identity, 87% coverage: 50:396/399 of query aligns to 388:708/711 of P96855
- E581 (= E244) mutation to Q: Displays less than 1% activity with cholyl-CoA as substrate. Still binds FAD.
Sites not aligning to the query:
- 236 R→A: Displays less than 2% activity with cholyl-CoA as substrate. Cannot bind FAD.
2pg0A Crystal structure of acyl-coa dehydrogenase from geobacillus kaustophilus
31% identity, 95% coverage: 12:392/399 of query aligns to 12:376/380 of 2pg0A
- active site: M124 (≠ Y128), T125 (≠ S129), E243 (= E244), A364 (≠ G380), R376 (≠ K392)
- binding flavin-adenine dinucleotide: I122 (≠ Q126), M124 (≠ Y128), T125 (≠ S129), G130 (= G134), S131 (= S135), F155 (≠ W159), I156 (≠ T160), T157 (= T161), R269 (≠ Q268), F272 (≠ R271), F279 (≠ N278), Q337 (≠ R336), L338 (≠ A337), G340 (= G339), G341 (≠ V340), V359 (≠ K375), I362 (= I378), Y363 (≠ F379), T366 (≠ S382), E368 (= E384), M369 (≠ I385)
4iv6B X-ray crystal structure of an isovaleryl-coa dehydrogenase from mycobacterium smegmatis (see paper)
32% identity, 75% coverage: 93:392/399 of query aligns to 86:373/383 of 4iv6B
- active site: L121 (≠ Y128), T122 (≠ S129), G240 (≠ E244), E361 (vs. gap), K373 (= K392)
- binding dihydroflavine-adenine dinucleotide: L121 (≠ Y128), T122 (≠ S129), G126 (≠ A133), G127 (= G134), S128 (= S135), W152 (= W159), I153 (≠ T160), S154 (≠ T161), R266 (≠ Q268), S268 (≠ H270), F269 (≠ R271), I273 (≠ L275), H276 (≠ N278), V279 (≠ F281), R334 (= R336), V335 (≠ A337), G338 (≠ V340), L356 (= L376), G360 (= G380), T363 (≠ S382), E365 (= E384), I366 (= I385)
3r7kA Crystal structure of a probable acyl coa dehydrogenase from mycobacterium abscessus atcc 19977 / dsm 44196 (see paper)
33% identity, 85% coverage: 52:392/399 of query aligns to 47:375/378 of 3r7kA
- active site: V126 (≠ Y128), T127 (≠ S129), E242 (= E244), G363 (= G380), K375 (= K392)
- binding dihydroflavine-adenine dinucleotide: V126 (≠ Y128), T127 (≠ S129), G132 (= G134), S133 (= S135), F157 (≠ W159), I158 (≠ T160), T159 (= T161), R268 (≠ Q268), T270 (≠ H270), F271 (≠ R271), L275 (= L275), R278 (≠ N278), I281 (≠ F281), Q336 (≠ G352), I337 (≠ D353), G340 (≠ A356), I358 (≠ K375), T365 (≠ S382), E367 (= E384)
3pfdC Crystal structure of an acyl-coa dehydrogenase from mycobacterium thermoresistibile bound to reduced flavin adenine dinucleotide solved by combined iodide ion sad mr (see paper)
30% identity, 87% coverage: 50:395/399 of query aligns to 37:368/369 of 3pfdC
- active site: L116 (≠ Y128), S117 (= S129), T233 (≠ E244), E353 (≠ G380), R365 (≠ K392)
- binding dihydroflavine-adenine dinucleotide: Y114 (≠ Q126), L116 (≠ Y128), S117 (= S129), G122 (= G134), S123 (= S135), W147 (= W159), I148 (≠ T160), T149 (= T161), R259 (≠ Q268), F262 (≠ R271), V266 (≠ L275), N269 (= N278), Q326 (≠ R336), L327 (≠ A337), G330 (≠ V340), I348 (≠ L376), Y352 (≠ F379), T355 (≠ S382), Q357 (≠ E384)
1ukwB Crystal structure of medium-chain acyl-coa dehydrogenase from thermus thermophilus hb8
31% identity, 86% coverage: 50:392/399 of query aligns to 45:374/379 of 1ukwB
- active site: L124 (≠ Y128), S125 (= S129), T241 (≠ E244), E362 (≠ G380), R374 (≠ K392)
- binding cobalt (ii) ion: D145 (= D149), H146 (= H150)
- binding flavin-adenine dinucleotide: F122 (≠ Q126), L124 (≠ Y128), S125 (= S129), G130 (= G134), S131 (= S135), W155 (= W159), S157 (≠ T161), K200 (vs. gap), L357 (= L376), Y361 (≠ F379), E362 (≠ G380), T364 (≠ S382), E366 (= E384), L370 (≠ N388)
1ukwA Crystal structure of medium-chain acyl-coa dehydrogenase from thermus thermophilus hb8
31% identity, 86% coverage: 50:392/399 of query aligns to 45:374/379 of 1ukwA
- active site: L124 (≠ Y128), S125 (= S129), T241 (≠ E244), E362 (≠ G380), R374 (≠ K392)
- binding flavin-adenine dinucleotide: F122 (≠ Q126), L124 (≠ Y128), S125 (= S129), G130 (= G134), S131 (= S135), W155 (= W159), S157 (≠ T161), L357 (= L376), Y361 (≠ F379), E362 (≠ G380), T364 (≠ S382), E366 (= E384), L370 (≠ N388)
8i4rA Crystal structure of acyl-coa dehydrogenase complexed with acetyl-coa from thermobifida fusca
28% identity, 98% coverage: 1:392/399 of query aligns to 1:377/381 of 8i4rA
- binding acetyl coenzyme *a: S132 (= S135), T134 (≠ L137), R180 (≠ E183), R234 (≠ T235), L237 (≠ K238), R238 (≠ Y239), L240 (= L241), D241 (≠ T242), R244 (= R245), E365 (≠ G380), G366 (= G381), R377 (≠ K392)
- binding flavin-adenine dinucleotide: Y123 (≠ Q126), L125 (≠ Y128), S126 (= S129), G131 (= G134), S132 (= S135), W156 (= W159), I157 (≠ T160), T158 (= T161), I360 (≠ L376), T367 (≠ S382), Q369 (≠ E384)
8i4pA Crystal structure of acyl-coa dehydrogenase from thermobifida fusca
28% identity, 98% coverage: 1:392/399 of query aligns to 1:377/381 of 8i4pA
- binding flavin-adenine dinucleotide: Y123 (≠ Q126), L125 (≠ Y128), S126 (= S129), G131 (= G134), S132 (= S135), W156 (= W159), I157 (≠ T160), T158 (= T161), I360 (≠ L376), Y364 (≠ F379), T367 (≠ S382), Q369 (≠ E384)
7w0jE Acyl-coa dehydrogenase, tfu_1647
28% identity, 98% coverage: 1:392/399 of query aligns to 2:378/382 of 7w0jE
- binding [[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2R,3S,4S)-5-azanyl-2,3,4-tris(oxidanyl)pentyl] hydrogen phosphate: S127 (= S129), W157 (= W159), R270 (≠ Q268), Q272 (≠ H270), F273 (≠ R271), I277 (≠ L275), F280 (≠ N278), I283 (≠ F281), Q339 (≠ R336), L340 (≠ A337), G343 (≠ V340), Y365 (≠ F379), E366 (≠ G380), T368 (≠ S382), Q370 (≠ E384), I371 (= I385)
6es9A Methylsuccinyl-coa dehydrogenase of paracoccus denitrificans with bound flavin adenine dinucleotide (see paper)
31% identity, 86% coverage: 50:392/399 of query aligns to 202:541/545 of 6es9A
- active site: F281 (≠ Y128), T282 (≠ S129), A408 (≠ T246), R541 (≠ K392)
- binding coenzyme a: F467 (≠ I303), W470 (vs. gap)
- binding flavin-adenine dinucleotide: A279 (≠ Q126), F281 (≠ Y128), T282 (≠ S129), G287 (= G134), S288 (= S135), W312 (= W159), I313 (≠ T160), T314 (= T161), E374 (= E210), R434 (≠ Q268), Q436 (≠ H270), F437 (≠ R271), L441 (= L275), F444 (≠ N278), Q502 (≠ R336), I503 (≠ A337), G505 (= G339), G506 (≠ V340), F528 (= F379), A531 (≠ S382), E533 (= E384), I534 (= I385)
Sites not aligning to the query:
5ol2F The electron transferring flavoprotein/butyryl-coa dehydrogenase complex from clostridium difficile (see paper)
27% identity, 86% coverage: 50:391/399 of query aligns to 44:373/378 of 5ol2F
- active site: L124 (≠ Y128), T125 (≠ S129), G241 (≠ E244)
- binding coenzyme a persulfide: L238 (= L241), R242 (= R245), E362 (≠ G380), G363 (= G381)
- binding flavin-adenine dinucleotide: F122 (≠ Q126), L124 (≠ Y128), T125 (≠ S129), P127 (= P131), T131 (≠ S135), F155 (≠ W159), I156 (≠ T160), T157 (= T161), E198 (≠ P201), R267 (≠ Q268), F270 (≠ R271), L274 (= L275), F277 (≠ N278), Q335 (≠ R336), L336 (≠ A337), G338 (= G339), G339 (≠ V340), Y361 (≠ F379), T364 (≠ S382), E366 (= E384)
Sites not aligning to the query:
5lnxD Crystal structure of mmgc, an acyl-coa dehydrogenase from bacillus subtilis.
29% identity, 87% coverage: 50:396/399 of query aligns to 42:374/374 of 5lnxD
- active site: L122 (≠ Y128), T123 (≠ S129), G239 (≠ E244), E358 (≠ G380), K370 (= K392)
- binding flavin-adenine dinucleotide: L122 (≠ Y128), T123 (≠ S129), G128 (= G134), S129 (= S135), F153 (≠ W159), T155 (= T161), R265 (≠ Q268), Q267 (≠ H270), F268 (≠ R271), I272 (≠ L275), N275 (= N278), I278 (≠ F281), Q331 (≠ R335), I332 (≠ R336), G335 (= G339), Y357 (≠ F379), T360 (≠ S382), E362 (= E384)
1jqiA Crystal structure of rat short chain acyl-coa dehydrogenase complexed with acetoacetyl-coa (see paper)
30% identity, 86% coverage: 50:391/399 of query aligns to 47:376/384 of 1jqiA
- binding acetoacetyl-coenzyme a: L95 (vs. gap), F125 (≠ Q126), S134 (= S135), F234 (≠ W234), M238 (≠ K238), Q239 (≠ Y239), L241 (= L241), D242 (≠ T242), R245 (= R245), Y364 (≠ F379), E365 (≠ G380), G366 (= G381)
- binding flavin-adenine dinucleotide: F125 (≠ Q126), L127 (≠ Y128), S128 (= S129), G133 (= G134), S134 (= S135), W158 (= W159), T160 (= T161), R270 (≠ Q268), F273 (≠ R271), L280 (≠ N278), Q338 (vs. gap), I339 (vs. gap), G342 (= G352), I360 (≠ L376), T367 (≠ S382), E369 (= E384), I370 (= I385)
Sites not aligning to the query:
8hk0B Crystal structure of fic32-33 complex from streptomyces ficellus nrrl 8067 (see paper)
30% identity, 85% coverage: 1:340/399 of query aligns to 1:329/379 of 8hk0B
Sites not aligning to the query:
Query Sequence
>GFF402 FitnessBrowser__Phaeo:GFF402
MDLNYSTEDVAFRAEVRAFLDAKLPQDLSDAVRLGRGLGKEGHDRWHSILNEQGWLAPTW
PEEYGGCGWNAVQRHIFEEECCRAHAPRIVPFGLTMLAPVLQKFGSDEQRAYFLPKILSG
EHWWCQGYSEPGAGSDLASLRTRAVRDGDHYVVNGQKTWTTLGQYANWIFCLVRTDPDAK
QQEGISFLLIDMDTPGIEVRPIILLDGTHEVNEVWFTDVRVPVENLVGEENKGWTYAKYL
LTHERTNIAGVGFSQAGLEAVKRLARQQIHRGRPLIENPHFAARLARAEIDLMAMATTNL
RIISAAAQGQAPGVESSMLKVKGTEIRQEINDLARRAAGVYALPFASEALAGDNSALAPP
EGSDAAAAQYFNNRKLSIFGGSNEIQKNIIAKISLGGRT
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory