SitesBLAST

C26 (≠ Q14) mutation to A: Lower ATPase activity and transport efficiency.
F27 (= F15) mutation to L: Lower ATPase activity and transport efficiency.
F45 (= F33) mutation to L: Lower ATPase activity and transport efficiency.
C54 (= C42) mutation to T: Loss of ATPase activity and transport.
L60 (= L48) mutation to F: Lower ATPase activity and transport efficiency.
L76 (= L64) mutation to P: Lower ATPase activity and transport efficiency.
V135 (= V123) mutation to M: Loss of ATPase activity and transport.
D172 (= D160) mutation to N: Loss of ATPase activity and transport.
C276 (≠ R263) mutation to A: Lower ATPase activity and transport efficiency.
E297 (= E285) mutation E->K,D: Lower ATPase activity and transport efficiency.; mutation to Q: Loss of ATPase activity and transport.

2d62A Crystal structure of multiple sugar binding transport atp- binding protein
44% identity, 87% coverage: 6:329/373 of query aligns to 7:342/375 of 2d62A

query
sites
2d62A

L

V

S

K

L

L

S

I

N

N

L

I

S

W

K

K

Q

R

F

F

G

G

G

D

K

V

T

T

V

A

L

V

D

K

G

D

L

L

D

S

L

L

E

E

I

I

Y

K

D

D

G

G

E

E

F

F

I

L

T

V

L

L

G

G

P

P

S

S

G
|
G

C
|
C

G
|
G

K
|
K

T
|
T

L

T

L

L

R

R

L

M

M

I

A

A

G

G

F

L

E

E

H

E

P

P

D

T

E

R

G

G

T

Q

I

I

T

Y

L

I

A

E

G

D

-

N

-

L

-

V

-

A

-

D

-

P

E

E

N

K

L

G

T

V

H

F

T

V

A

P

P

P

E

K

N

E

R

R

P

D

L

V

N

A

T

M

V

V

F

F

Q

Q

H

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

M

S

T

V

V

F

Y

D

D

N

N

V

I

A

A

Y

F

G

P

L

L

K

K

M

L

E

R

K

K

R

V

P

P

K

K

D

Q

E

E

I

I

R

D

Q

K

R

R

V

V

D

R

E

E

A

V

L

A

A

E

M

M

V

L

Q

G

L

L

Q

T

D

E

F

L

A

L

R

N

R

R

K

K

P

P

H

R

Q

E

L

L

S

S

G

G

Q

Q

Q

R

Q

Q

R

R

V

V

A

A

I

L

A

G

R

R

A

A

V

I

K

R

R

R

P

P

R

K

L

V

L

F

L

L

L

M

D

D

E

E

P

P

L

L

S

S

A

N

L

L

D

D

Y

A

K

K

L

L

R

R

R

V

T

K

M

M

Q

R

V

A

E

E

L

L

K

K

R

K

L

L

Q

Q

R

R

E

Q

L

L

G

G

I

V

T

T

F

T

V

I

F

Y

V

V

T

T

H

H

D

D

Q

Q

E

V

E

E

A

A

L

M

S

T

M

M

S

G

D

D

R

R

V

I

V

A

V

V

L

M

K

N

D

K

G

G

L

E

V

L

Q

Q

L

V

G

G

T

T

P

P

R

D

E

E

V

V

Y

Y

E

Y

R

K

P

P

A

V

N

N

L

T

F

F

T

V

A

A

R

G

F

F

V

I

G

G

E

S

-

P

T

M

N

N

F

F

F

L

P

D

G

A

T

T

V

I

E

-

S

-

V

T

Q

D

D

D

G

G

S

F

I

L

K

D

V

F

D

G

V

E

F

F

G

K

L

L

K

K

R

-

T

-

L

L

R

L

R

Q

P

D

D

Q

F

F

P

E

V

V

Q

L

A

E

E

E

Q

E

S

N

L

M

-

V

-

G

-

K

H

E

V

V

L

I

-

F

-

G

L

I

R

R

P

P

E

E

D

D

I

V

R

H

-

D

-

A

-

S

-

F

-

T

V

H

L

I

E

D

P

V

D

P

D

E

E

E

N

N

G

T

V

V

A

K

G

A

K

T

I

V

-

D

V

I

E

I

R

E

N

N

Y

L

K

G

G

G

S

-

T

-

L

-

D

E

S

K

V

I

I

V

H

H

L

L

A

R

D

R

G

G

T

N

E

I

V

S

L

F

A

T

S

A

E

K

F

F

binding pyrophosphate 2-: G42 (= G41), C43 (= C42), G44 (= G43), K45 (= K44), T46 (= T45), T47 (= T46)

1g291 Malk (see paper)
46% identity, 76% coverage: 6:287/373 of query aligns to 4:294/372 of 1g291

query
sites
1g291

L

V

S

R

L

L

S

V

N

D

L

V

S

W

K

K

Q

V

F

F

G

G

G

E

K

V

T

T

V

A

L

V

D

R

G

E

L

M

D

S

L

L

E

E

I

V

Y

K

D

D

G

G

E

E

F

F

I

M

T

I

L

L

G

G

P

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
|
T

L

T

L

L

R

R

L

M

M

I

A

A

G

G

F

L

E

E

H

E

P

P

D

S

E

R

G

G

T

Q

I

I

T

Y

L

I

A

G

G

D

-

K

-

L

-

V

-

A

-
x
D

-

P

E
|
E

N
x
K

L

G

T

I

H

F

T

V

A

P

P

P

E
x
K

N
x
D

R

R

P

D

L

I

N

A

T

M

V

V

F

F

Q

Q

H

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

M

S

T

V

V

F

Y

D

D

N

N

V

I

A

A

Y

F

G

P

L

L

K

K

M

L

E

R

K

K

R

V

P

P

K

R

D

Q

E

E

I

I

R

D

Q

Q

R

R

V

V

D

R

E

E

A

V

L

A

A

E

M

L

V

L

Q

G

L

L

Q

T

D

E

F

L

A

L

R

N

R

R

K

K

P

P

H

R

Q

E

L

L

S

S

G

G

Q

Q

Q

R

Q

Q

R

R

V

V

A

A

I

L

A

G

R

R

A

A

V

I

V

V

K

R

R

K

P

P

R

Q

L

V

L

F

L

L

L

M

D

D

E

E

P

P

L

L

S

S

A

N

L

L

D

D

Y

A

K

K

L

L

R

R

R

V

T

R

M

M

Q

R

V

A

E

E

L

L

K

K

R

K

L

L

Q

Q

R

R

E

Q

L

L

G

G

I

V

T

T

F

T

V

I

F

Y

V

V

T

T

H

H

D

D

Q

Q

E

V

E

E

A

A

L

M

S

T

M

M

S

G

D

D

R

R

V

I

V

A

V

V

L

M

K

N

D

R

G

G

L

V

V

L

Q

Q

L

V

G

G

T

S

P

P

R

D

E

E

V

V

Y

Y

E

D

R

K

P

P

A

A

N

N

L

T

F

F

T

V

A

A

R

G

F

F

V

I

G

G

E

S

-

P

T

M

N

N

F

F

F

L

P

D

G

A

T

I

V

V

E

-

S

-

V

T

Q

E

D

D

G

G

S

F

I

V

K

D

V

F

D

G

V

E

F

F

G

R

L

L

K

K

R

L

T

L

L

-

R

-

P

P

D

D

-

Q

F

F

P

E

V

V

Q

L

A

G

E

E

-

L

-

G

-

Y

-

V

-

G

Q

R

S

E

L

V

H

I

V

F

L

G

L

I

R

R

P

P

E
|
E

D
|
D

I

L

binding magnesium ion: D69 (vs. gap), E71 (= E67), K72 (≠ N68), K79 (≠ E75), D80 (≠ N76), E292 (= E285), D293 (= D286)
binding pyrophosphate 2-: S38 (= S40), G39 (= G41), C40 (= C42), G41 (= G43), K42 (= K44), T43 (= T45), T44 (= T46)

Sites not aligning to the query:

binding magnesium ion: 359

P9WQI3 Trehalose import ATP-binding protein SugC; MtbSugC; Nucleotide-binding domain of SugABC transporter; NBD of SugABC transporter; SugABC transporter ATPase SugC; EC 7.5.2.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
50% identity, 65% coverage: 6:249/373 of query aligns to 4:250/393 of P9WQI3

query
sites
P9WQI3

L

I

S

V

L

L

S

D

N

H

L

V

S

N

K

K

Q

S

F

Y

-

P

G

D

G

G

K

H

T

T

V

A

L

V

D

R

G

D

L

L

D

N

L

L

E

T

I

I

Y

A

D

D

G

G

E

E

F

F

I

L

T

I

L

L

L

V

G

G

P

P

S

S

G

G

C

C

G

G

K

K

T

T

L

T

L

L

R

N

L

M

M

I

A

A

G

G

F

L

E

E

H

D

P

I

D

S

E

S

G

G

T

E

I

L

T

R

L

I

A

A

G

G

E

E

N

R

L

V

T

N

H

E

T

K

A

A

P

P

E

K

N

D

R

R

P

D

L

I

N

A

T

M

V

V

F

F

Q

Q

H

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

M

S

T

V

V

F

R

D

Q

N

N

V

I

A

A

Y

F

G

P

L

L

K

T

M

L

E

A

K

K

R

M

P

R

K

K

D

A

E

D

I

I

R

A

Q

Q

R

K

V

V

D

S

E

E

A

T

L

A

A

K

M

I

V

L

Q

D

L

L

Q

T

D

N

F

L

A

L

R

D

R

R

K

K

P

P

H

S

Q

Q

L

L

S

S

G

G

Q

Q

Q

R

Q

Q

R

R

V

V

A

A

I

M

A

G

R

R

A

A

V

I

V

V

K

R

R

H

P

P

R

K

L

A

L

F

L

L

L

M

D

D

E

E

P

P

L

L

S

S

A

N

L

L

D

D

Y

A

K

K

L

L

R

R

R

V

T

Q

M

M

Q

R

V

G

E

E

L

I

K

A

R

Q

L

L

Q

Q

R

R

E

R

L

L

G

G

I

T

T

T

F

T

V

V

F

Y

V

V

T

T

H
|
H

D

D

Q

Q

E

T

E

E

A

A

L

M

S

T

M

L

S

G

D

D

R

R

V

V

L

M

K

Y

D

G

G

G

L

I

V

A

Q

Q

L

I

G

G

T

T

P

P

R

E

E

E

V

L

Y

Y

E

E

R

R

P

P

A

A

N

N

L

L

F

F

T

V

A

A

R

G

F

F

V

I

G

G

E

S

-

P

-

A

T

M

N

N

F

F

P

P

G

A

T

R

V

L

E

T

S

A

V

I

H193 (= H194) mutation to A: Decreased hydrolysis of ATP. No change in KM, but 2-fold reduction in Vmax compared to wild-type.

8hplC Lpqy-sugabc in state 1 (see paper)
52% identity, 60% coverage: 24:245/373 of query aligns to 20:243/384 of 8hplC

query
sites
8hplC

L

F

D

S

L

M

E

T

I

I

Y

A

D

D

G

G

E

E

F

F

I

I

T

I

L

L

L

V

G

G

P

P

S

S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

L

T

L

L

R

N

L

M

M

I

A

A

G

G

F

L

E

E

H

E

P

I

D

T

E

S

G

G

T

E

I

L

T

R

L

I

A

G

G

G

E

E

N

R

L

V

T

N

H

E

T

K

A

A

P

P

E

K

N

D

R

R

P

D

L

I

N

A

T

M

V

V

F

F

Q

Q

H

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

M

S

T

V

V

F

R

D

Q

N

N

V

I

A

A

Y

F

G

P

L

L

K

T

M

L

E

A

K

K

R

V

P

P

K

K

D

A

E

E

I

I

R

A

Q

A

R

K

V

V

D

E

E

E

A

T

L

A

A

K

M

I

V

L

Q

D

L

L

Q

S

D

E

F

L

A

L

R

D

R

R

K

K

P

P

H

G

Q

Q

L

L

S

S

G

G

Q

Q

Q

R

Q

Q

R

R

V

V

A

A

I

M

A

G

R

R

A

A

V

I

V

V

K

R

R

S

P

P

R

K

L

A

L

F

L

L

L

M

D

D

E

Q

P

P

L

L

S

S

A

N

L

L

D

D

Y

A

K

K

L

L

R

R

R

V

T

Q

M

M

Q

R

V

A

E

E

L

I

K

S

R

R

L

L

Q

Q

R

D

E

R

L

L

G

G

I

T

T

T

F

T

V

V

F

Y

V

V

T

T

H

H

D

D

Q

Q

E

T

E

E

A

A

L

M

S

T

M

L

S

G

D

D

R

R

V

V

L

M

K

L

D

A

G

G

L

E

V

V

Q

Q

L

I

G

G

T

T

P

P

R

D

E

E

V

L

Y

Y

E

S

R

S

P

P

A

A

N

N

L

L

F

F

T

V

A

A

R

G

F

F

V

I

G

G

E

S

-

P

-

A

T

M

N

N

F

F

P

P

G

A

T

T

binding adenosine-5'-triphosphate: G37 (= G41), C38 (= C42), G39 (= G43), K40 (= K44), S41 (≠ T45), T42 (= T46)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 12

8hprC Lpqy-sugabc in state 4 (see paper)
52% identity, 60% coverage: 24:245/373 of query aligns to 22:245/363 of 8hprC

query
sites
8hprC

L

F

D

S

L

M

E

T

I

I

Y

A

D

D

G

G

E

E

F

F

I

I

T

I

L

L

L

V

G

G

P

P

S
|
S

G
|
G

C

C

G
|
G

K
|
K

T
x
S

T

T

L

T

L

L

R

N

L

M

M

I

A

A

G

G

F

L

E

E

H

E

P

I

D

T

E

S

G

G

T

E

I

L

T

R

L

I

A

G

G

G

E

E

N

R

L

V

T

N

H

E

T

K

A

A

P

P

E

K

N

D

R

R

P

D

L

I

N

A

T

M

V

V

F

F

Q
|
Q

H

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

M

S

T

V

V

F

R

D

Q

N

N

V

I

A

A

Y

F

G

P

L

L

K

T

M

L

E

A

K

K

R

V

P

P

K

K

D

A

E

E

I

I

R

A

Q

A

R

K

V

V

D

E

E

E

A

T

L

A

A

K

M

I

V

L

Q

D

L

L

Q

S

D

E

F

L

A

L

R

D

R

R

K

K

P

P

H

G

Q
|
Q

L

L

S

S

G
|
G

Q
|
Q

Q

R

Q

Q

R

R

V

V

A

A

I

M

A

G

R

R

A

A

V

I

V

V

K

R

R

S

P

P

R

K

L

A

L

F

L

L

L

M

D

D

E

Q

P

P

L

L

S

S

A

N

L

L

D

D

Y

A

K

K

L

L

R

R

R

V

T

Q

M

M

Q

R

V

A

E

E

L

I

K

S

R

R

L

L

Q

Q

R

D

E

R

L

L

G

G

I

T

T

T

F

T

V

V

F

Y

V

V

T

T

H
|
H

D

D

Q

Q

E

T

E

E

A

A

L

M

S

T

M

L

S

G

D

D

R

R

V

V

L

M

K

L

D

A

G

G

L

E

V

V

Q

Q

L

I

G

G

T

T

P

P

R

D

E

E

V

L

Y

Y

E

S

R

S

P

P

A

A

N

N

L

L

F

F

T

V

A

A

R

G

F

F

V

I

G

G

E

S

-

P

-

A

T

M

N

N

F

F

P

P

G

A

T

T

binding adenosine-5'-triphosphate: S38 (= S40), G39 (= G41), G41 (= G43), K42 (= K44), S43 (≠ T45), Q82 (= Q84), Q133 (= Q135), G136 (= G138), G137 (= G139), Q138 (= Q140), H192 (= H194)
binding magnesium ion: S43 (≠ T45), Q82 (= Q84)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 12

8hprD Lpqy-sugabc in state 4 (see paper)
52% identity, 60% coverage: 24:245/373 of query aligns to 22:245/362 of 8hprD

query
sites
8hprD

L

F

D

S

L

M

E

T

I

I

Y

A

D

D

G

G

E

E

F

F

I

I

T

I

L

L

L

V

G

G

P

P

S
|
S

G

G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

L

T

L

L

R

N

L

M

M

I

A

A

G

G

F

L

E

E

H

E

P

I

D

T

E

S

G

G

T

E

I

L

T

R

L

I

A

G

G

G

E

E

N

R

L

V

T

N

H

E

T

K

A

A

P

P

E

K

N

D

R

R

P

D

L

I

N

A

T

M

V

V

F

F

Q
|
Q

H

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

M

S

T

V

V

F

R

D

Q

N

N

V

I

A

A

Y

F

G

P

L

L

K

T

M

L

E

A

K

K

R

V

P

P

K

K

D

A

E

E

I

I

R

A

Q

A

R

K

V

V

D

E

E

E

A

T

L

A

A

K

M

I

V

L

Q

D

L

L

Q

S

D

E

F

L

A

L

R

D

R
|
R

K

K

P

P

H

G

Q
|
Q

L

L

S
|
S

G
|
G

Q

Q

Q

R

Q

Q

R

R

V

V

A

A

I

M

A

G

R

R

A

A

V

I

V

V

K

R

R

S

P

P

R

K

L

A

L

F

L

L

L

M

D

D

E
x
Q

P

P

L

L

S

S

A

N

L

L

D

D

Y

A

K

K

L

L

R

R

R

V

T

Q

M

M

Q

R

V

A

E

E

L

I

K

S

R

R

L

L

Q

Q

R

D

E

R

L

L

G

G

I

T

T

T

F

T

V

V

F

Y

V

V

T

T

H
|
H

D

D

Q

Q

E

T

E

E

A

A

L

M

S

T

M

L

S

G

D

D

R

R

V

V

L

M

K

L

D

A

G

G

L

E

V

V

Q

Q

L

I

G

G

T

T

P

P

R

D

E

E

V

L

Y

Y

E

S

R

S

P

P

A

A

N

N

L

L

F

F

T

V

A

A

R

G

F

F

V

I

G

G

E

S

-

P

-

A

T

M

N

N

F

F

P

P

G

A

T

T

binding adenosine-5'-triphosphate: S38 (= S40), C40 (= C42), G41 (= G43), K42 (= K44), S43 (≠ T45), T44 (= T46), Q82 (= Q84), R129 (= R131), Q133 (= Q135), S135 (= S137), G136 (= G138), G137 (= G139), Q159 (≠ E161), H192 (= H194)
binding magnesium ion: S43 (≠ T45), Q82 (= Q84)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 12

1vciA Crystal structure of the atp-binding cassette of multisugar transporter from pyrococcus horikoshii ot3 complexed with atp (see paper)
50% identity, 64% coverage: 1:237/373 of query aligns to 2:230/353 of 1vciA

query
sites
1vciA

M

I

K

K

Q

M

T

V

L

E

L

V

S

K

L

L

S

E

N

N

L

L

S

T

K

K

Q

R

F
|
F

G

G

G

N

K
x
F

T

T

V

A

L

V

D

N

G

K

L

L

D

N

L

L

E

T

I

I

Y

K

D

D

G

G

E

E

F

F

I

L

T

V

L

L

G

G

P

P

S
|
S

G
|
G

C

C

G
|
G

K
|
K

T
|
T

L

T

L

L

R

R

L

M

M

I

A

A

G

G

F

L

E

E

H

E

P

P

D

T

E

E

G

G

T

R

I

I

T

Y

L

F

A

G

G

D

E

R

N

D

L

V

T

T

H

Y

T

L

A

P

P

P

E

K

N

D

R

R

P

N

L

I

N

S

T

M

V

V

F

F

Q

Q

H

H

Y

-

A

-

L

-

F

-

P

-

H

-

M

M

S

T

V

V

F

Y

D

E

N

N

V

I

A

A

Y

F

G

P

L

L

K

K

M

-

E

-

K

K

R

F

P

P

K

K

D

D

E

E

I

I

R

D

Q

K

R

R

V

V

D

R

E

W

A

A

L

A

A

E

M

L

V

L

Q

Q

L

I

Q

E

D

E

F

L

A

L

R

N

R

R

K

Y

P

P

H

A

Q

Q

L

L

S

S

G

G

Q

Q

Q

R

Q

Q

R

R

V

V

A

A

I

V

A

A

R

R

A

A

V

I

V

V

K

V

R

E

P

P

R

D

L

V

L

L

L

M

D

D

E

E

P

P

L

L

S

S

A

N

L

L

D

D

Y

A

K

K

L

L

R

R

R

V

T

A

M

M

Q

R

V

A

E

E

L

I

K

K

R

K

L

L

Q

Q

R

Q

E

K

L

L

G

K

I

V

T

T

F

T

V

I

F

Y

V

V

T

T

H

H

D

D

Q

Q

E

V

E

E

A

A

L

M

S

T

M

M

S

G

D

D

R

R

V

I

V

A

V

V

L

M

K

N

D

R

G

G

L

Q

V

L

Q

L

Q

Q

L

I

G

G

T

S

P

P

R

T

E

E

V

V

Y

Y

E

L

R

R

P

P

A

N

N

S

L

V

F

F

T

V

A

A

R

T

F

F

V

I

G

G

binding adenosine-5'-triphosphate: F16 (= F15), F19 (≠ K18), S41 (= S40), G42 (= G41), G44 (= G43), K45 (= K44), T46 (= T45), T47 (= T46)

P19566 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
44% identity, 68% coverage: 6:258/373 of query aligns to 4:258/369 of P19566

query
sites
P19566

L

V

S

Q

L

L

S

R

N

N

L

V

S

T

K

K

Q

A

F

W

G

G

G

D

K

V

T

V

V

V

L

S

D

K

G

D

L

I

D

N

L

L

E

D

I

I

Y

H

D

D

G

G

E

E

F

F

I

V

T

V

L

F

L

V

G

G

P

P

S

S

G

G

C

C

G

G

K

K

T

S

T

T

L

L

R

R

L

M

M

I

A

A

G

G

F

L

E

E

H

T

P

I

D

T

E

S

G

G

T

D

I

L

T

F

L

I

A

G

G

E

E

T

N

R

L

M

T

N

H

D

T

I

A

P

P

P

E

A

N

E

R

R

P

G

L

V

N

G

T

M

V

V

F

F

Q

Q

H

S

Y

Y

A

A

L
|
L

F

Y

P

P

H

H

M

L

S

S

V

V

F

A

D

E

N

N

V

M

A

S

Y

F

G

G

L

L

K

K

M

L

E

A

K

G

R

A

P

K

K

K

D

E

E

V

I

M

R

N

Q

Q

R

R

V

V

D

N

E

Q

A

V

L

A

A

E

M

V

V

L

Q

Q

L

L

Q

A

D

H

F

L

A

L

R

E

R

R

K

K

P

P

H

K

Q

A

L

L

S

S

G

G

Q

Q

Q

R

Q

Q

R

R

V

V

A

A

I

I

A

G

R

R

A

T

V

L

V

V

K

A

R

E

P

P

R

R

L

V

L

F

L

L

D

D

E

E

P
|
P

L

L

S

S

A

N

L

L

D
|
D

Y

A

K

A

L

L

R

R

R

V

T

Q

M

M

Q

R

V

I

E

E

L

I

K

S

R

R

L

L

Q

H

R

K

E

R

L

L

G

G

I

R

T

T

F

M

V

I

F

Y

V

V

T

T

H

H

D

D

Q

Q

E

V

E

E

A

A

L

M

S

T

M

L

S

A

D

D

R

K

V

I

V

V

L

L

K

D

D

A

G

G

L

R

V

V

Q

A

Q

Q

L

V

G

G

T

K

P

P

R

L

E

E

V

L

Y

Y

E

H

R

Y

P

P

A

A

N

D

L

R

F

F

T

V

A

A

R

G

F

F

V

I

G

G

-

S

-

P

E

K

T

M

N

N

F

F

F

L

P

P

G

V

T

K

V

V

E

T

S

A

V

T

Q

A

D

I

G

E

S

Q

I

V

K

Q

V

V

D

E

V

L

L86 (= L88) mutation to F: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
P160 (= P162) mutation to L: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
D165 (= D167) mutation to N: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.

Sites not aligning to the query:

306 E→K: Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.

2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
44% identity, 68% coverage: 6:258/373 of query aligns to 3:257/374 of 2awnB

query
sites
2awnB

L

V

S

Q

L

L

S

Q

N

N

L

V

S

T

K

K

Q

A

F
x
W

G

G

G

E

K

V

T

V

V
|
V

L

S

D

K

G

D

L

I

D

N

L

L

E

D

I

I

Y

H

D

E

G

G

E

E

F

F

I

V

T

V

L

F

L

V

G

G

P

P

S
|
S

G
|
G

C

C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

R

R

L

M

M

I

A

A

G

G

F

L

E

E

H

T

P

I

D

T

E

S

G

G

T

D

I

L

T

F

L

I

A

G

G

E

E

K

N

R

L

M

T

N

H

D

T

T

A

P

P

P

E

A

N

E

R

R

P

G

L

V

N

G

T

M

V

V

F

F

Q

Q

H

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

L

S

S

V

V

F

A

D

E

N

N

V

M

A

S

Y

F

G

G

L

L

K

K

M

L

E

A

K

G

R

A

P

K

K

K

D

E

E

V

I

I

R

N

Q

Q

R

R

V

V

D

N

E

Q

A

V

L

A

A

E

M

V

V

L

Q

Q

L

L

Q

A

D

H

F

L

A

L

R

D

R

R

K

K

P

P

H

K

Q

A

L

L

S

S

G

G

Q

Q

Q

R

Q

Q

R

R

V

V

A

A

I

I

A

G

R

R

A

T

V

L

V

V

K

A

R

E

P

P

R

S

L

V

L

F

L

L

D

D

E

E

P

P

L

L

S

S

A

N

L

L

D

D

Y

A

K

A

L

L

R

R

R

V

T

Q

M

M

Q

R

V

I

E

E

L

I

K

S

R

R

L

L

Q

H

R

K

E

R

L

L

G

G

I

R

T

T

F

M

V

I

F

Y

V

V

T

T

H

H

D

D

Q

Q

E

V

E

E

A

A

L

M

S

T

M

L

S

A

D

D

R

K

V

I

V

V

L

L

K

D

D

A

G

G

L

R

V

V

Q

A

Q

Q

L

V

G

G

T

K

P

P

R

L

E

E

V

L

Y

Y

E

H

R

Y

P

P

A

A

N

D

L

R

F

F

T

V

A

A

R

G

F

F

V

I

G

G

-

S

-

P

E

K

T

M

N

N

F

F

F

L

P

P

G

V

T

K

V

V

E

T

S

A

V

T

Q

A

D

I

G

D

S

Q

I

V

K

Q

V

V

D

E

V

L

binding adenosine-5'-diphosphate: W12 (≠ F15), V17 (= V20), S37 (= S40), G38 (= G41), G40 (= G43), K41 (= K44), S42 (≠ T45), T43 (= T46)

1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
44% identity, 68% coverage: 6:258/373 of query aligns to 1:255/367 of 1q12A

query
sites
1q12A

L

V

S

Q

L

L

S

Q

N

N

L

V

S

T

K

K

Q

A

F
x
W

G

G

G

E

K

V

T

V

V

V

L

S

D

K

G

D

L

I

D

N

L

L

E

D

I

I

Y

H

D

E

G

G

E

E

F

F

I

V

T

V

L

F

L

V

G

G

P

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

R

R

L

M

M

I

A

A

G

G

F

L

E

E

H

T

P

I

D

T

E

S

G

G

T

D

I

L

T

F

L

I

A

G

G

E

E

K

N

R

L

M

T

N

H

D

T

T

A

P

P

P

E

A

N

E

R

R

P

G

L

V

N

G

T

M

V

V

F

F

Q

Q

H

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

L

S

S

V

V

F

A

D

E

N

N

V

M

A

S

Y

F

G

G

L

L

K

K

M

L

E

A

K

G

R

A

P

K

K

K

D

E

E

V

I

I

R

N

Q

Q

R

R

V

V

D

N

E

Q

A

V

L

A

A

E

M

V

V

L

Q

Q

L

L

Q

A

D

H

F

L

A

L

R

D

R
|
R

K

K

P

P

H

K

Q
x
A

L

L

S
|
S

G

G

G
|
G

Q
|
Q

Q

R

Q

Q

R

R

V

V

A

A

I

I

A

G

R

R

A

T

V

L

V

V

K

A

R

E

P

P

R

S

L

V

L

F

L

L

D

D

E

E

P

P

L

L

S

S

A

N

L

L

D

D

Y

A

K

A

L

L

R

R

R

V

T

Q

M

M

Q

R

V

I

E

E

L

I

K

S

R

R

L

L

Q

H

R

K

E

R

L

L

G

G

I

R

T

T

F

M

V

I

F

Y

V

V

T

T

H

H

D

D

Q

Q

E

V

E

E

A

A

L

M

S

T

M

L

S

A

D

D

R

K

V

I

V

V

L

L

K

D

D

A

G

G

L

R

V

V

Q

A

Q

Q

L

V

G

G

T

K

P

P

R

L

E

E

V

L

Y

Y

E

H

R

Y

P

P

A

A

N

D

L

R

F

F

T

V

A

A

R

G

F

F

V

I

G

G

-

S

-

P

E

K

T

M

N

N

F

F

F

L

P

P

G

V

T

K

V

V

E

T

S

A

V

T

Q

A

D

I

G

D

S

Q

I

V

K

Q

V

V

D

E

V

L

binding adenosine-5'-triphosphate: W10 (≠ F15), S35 (= S40), G36 (= G41), C37 (= C42), G38 (= G43), K39 (= K44), S40 (≠ T45), T41 (= T46), R126 (= R131), A130 (≠ Q135), S132 (= S137), G134 (= G139), Q135 (= Q140)

3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
44% identity, 68% coverage: 6:258/373 of query aligns to 3:257/371 of 3puyA

query
sites
3puyA

L

V

S

Q

L

L

S

Q

N

N

L

V

S

T

K

K

Q

A

F
x
W

G

G

G

E

K

V

T

V

V

V

L

S

D

K

G

D

L

I

D

N

L

L

E

D

I

I

Y

H

D

E

G

G

E

E

F

F

I

V

T

V

L

F

L

V

G

G

P

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

R

R

L

M

M

I

A

A

G

G

F

L

E

E

H

T

P

I

D

T

E

S

G

G

T

D

I

L

T

F

L

I

A

G

G

E

E

K

N

R

L

M

T

N

H

D

T

T

A

P

P

P

E

A

N

E

R

R

P

G

L

V

N

G

T

M

V

V

F

F

Q
|
Q

H

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

L

S

S

V

V

F

A

D

E

N

N

V

M

A

S

Y

F

G

G

L

L

K

K

M

L

E

A

K

G

R

A

P

K

K

K

D

E

E

V

I

I

R

N

Q

Q

R

R

V

V

D

N

E

Q

A

V

L

A

A

E

M

V

V

L

Q

Q

L

L

Q

A

D

H

F

L

A

L

R

D

R
|
R

K

K

P

P

H

K

Q
x
A

L

L

S
|
S

G

G

G
|
G

Q
|
Q

Q

R

Q

Q

R

R

V

V

A

A

I

I

A

G

R

R

A

T

V

L

V

V

K

A

R

E

P

P

R

S

L

V

L

F

L

L

D

D

E
|
E

P

P

L

L

S

S

A

N

L

L

D

D

Y

A

K

A

L

L

R

R

R

V

T

Q

M

M

Q

R

V

I

E

E

L

I

K

S

R

R

L

L

Q

H

R

K

E

R

L

L

G

G

I

R

T

T

F

M

V

I

F

Y

V

V

T

T

H
|
H

D

D

Q

Q

E

V

E

E

A

A

L

M

S

T

M

L

S

A

D

D

R

K

V

I

V

V

L

L

K

D

D

A

G

G

L

R

V

V

Q

A

Q

Q

L

V

G

G

T

K

P

P

R

L

E

E

V

L

Y

Y

E

H

R

Y

P

P

A

A

N

D

L

R

F

F

T

V

A

A

R

G

F

F

V

I

G

G

-

S

-

P

E

K

T

M

N

N

F

F

F

L

P

P

G

V

T

K

V

V

E

T

S

A

V

T

Q

A

D

I

G

D

S

Q

I

V

K

Q

V

V

D

E

V

L

binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ F15), S37 (= S40), G38 (= G41), C39 (= C42), G40 (= G43), K41 (= K44), S42 (≠ T45), T43 (= T46), Q81 (= Q84), R128 (= R131), A132 (≠ Q135), S134 (= S137), G136 (= G139), Q137 (= Q140), E158 (= E161), H191 (= H194)
binding magnesium ion: S42 (≠ T45), Q81 (= Q84)

3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
44% identity, 68% coverage: 6:258/373 of query aligns to 3:257/371 of 3puxA

query
sites
3puxA

L

V

S

Q

L

L

S

Q

N

N

L

V

S

T

K

K

Q

A

F
x
W

G

G

G

E

K

V

T

V

V

V

L

S

D

K

G

D

L

I

D

N

L

L

E

D

I

I

Y

H

D

E

G

G

E

E

F

F

I

V

T

V

L

F

L

V

G

G

P

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

R

R

L

M

M

I

A

A

G

G

F

L

E

E

H

T

P

I

D

T

E

S

G

G

T

D

I

L

T

F

L

I

A

G

G

E

E

K

N

R

L

M

T

N

H

D

T

T

A

P

P

P

E

A

N

E

R

R

P

G

L

V

N

G

T

M

V

V

F

F

Q
|
Q

H

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

L

S

S

V

V

F

A

D

E

N

N

V

M

A

S

Y

F

G

G

L

L

K

K

M

L

E

A

K

G

R

A

P

K

K

K

D

E

E

V

I

I

R

N

Q

Q

R

R

V

V

D

N

E

Q

A

V

L

A

A

E

M

V

V

L

Q

Q

L

L

Q

A

D

H

F

L

A

L

R

D

R
|
R

K

K

P

P

H

K

Q

A

L

L

S
|
S

G

G

G
|
G

Q
|
Q

Q

R

Q

Q

R

R

V

V

A

A

I

I

A

G

R

R

A

T

V

L

V

V

K

A

R

E

P

P

R

S

L

V

L

F

L

L

D

D

E

E

P

P

L

L

S

S

A

N

L

L

D

D

Y

A

K

A

L

L

R

R

R

V

T

Q

M

M

Q

R

V

I

E

E

L

I

K

S

R

R

L

L

Q

H

R

K

E

R

L

L

G

G

I

R

T

T

F

M

V

I

F

Y

V

V

T

T

H
|
H

D

D

Q

Q

E

V

E

E

A

A

L

M

S

T

M

L

S

A

D

D

R

K

V

I

V

V

L

L

K

D

D

A

G

G

L

R

V

V

Q

A

Q

Q

L

V

G

G

T

K

P

P

R

L

E

E

V

L

Y

Y

E

H

R

Y

P

P

A

A

N

D

L

R

F

F

T

V

A

A

R

G

F

F

V

I

G

G

-

S

-

P

E

K

T

M

N

N

F

F

F

L

P

P

G

V

T

K

V

V

E

T

S

A

V

T

Q

A

D

I

G

D

S

Q

I

V

K

Q

V

V

D

E

V

L

binding adenosine-5'-diphosphate: W12 (≠ F15), G38 (= G41), C39 (= C42), G40 (= G43), K41 (= K44), S42 (≠ T45), T43 (= T46), R128 (= R131), S134 (= S137), Q137 (= Q140)
binding beryllium trifluoride ion: S37 (= S40), G38 (= G41), K41 (= K44), Q81 (= Q84), S134 (= S137), G136 (= G139), H191 (= H194)
binding magnesium ion: S42 (≠ T45), Q81 (= Q84)

3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
44% identity, 68% coverage: 6:258/373 of query aligns to 3:257/371 of 3puwA

query
sites
3puwA

L

V

S

Q

L

L

S

Q

N

N

L

V

S

T

K

K

Q

A

F
x
W

G

G

G

E

K

V

T

V

V
|
V

L

S

D

K

G

D

L

I

D

N

L

L

E

D

I

I

Y

H

D

E

G

G

E

E

F

F

I

V

T

V

L

F

L

V

G

G

P

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

R

R

L

M

M

I

A

A

G

G

F

L

E

E

H

T

P

I

D

T

E

S

G

G

T

D

I

L

T

F

L

I

A

G

G

E

E

K

N

R

L

M

T

N

H

D

T

T

A

P

P

P

E

A

N

E

R

R

P

G

L

V

N

G

T

M

V

V

F

F

Q
|
Q

H

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

L

S

S

V

V

F

A

D

E

N

N

V

M

A

S

Y

F

G

G

L

L

K

K

M

L

E

A

K

G

R

A

P

K

K

K

D

E

E

V

I

I

R

N

Q

Q

R

R

V

V

D

N

E

Q

A

V

L

A

A

E

M

V

V

L

Q

Q

L

L

Q

A

D

H

F

L

A

L

R

D

R
|
R

K

K

P

P

H

K

Q
x
A

L

L

S
|
S

G
|
G

Q
|
Q

Q

R

Q

Q

R

R

V

V

A

A

I

I

A

G

R

R

A

T

V

L

V

V

K

A

R

E

P

P

R

S

L

V

L

F

L

L

D

D

E
|
E

P

P

L

L

S

S

A

N

L

L

D

D

Y

A

K

A

L

L

R

R

R

V

T

Q

M

M

Q

R

V

I

E

E

L

I

K

S

R

R

L

L

Q

H

R

K

E

R

L

L

G

G

I

R

T

T

F

M

V

I

F

Y

V

V

T

T

H
|
H

D

D

Q

Q

E

V

E

E

A

A

L

M

S

T

M

L

S

A

D

D

R

K

V

I

V

V

L

L

K

D

D

A

G

G

L

R

V

V

Q

A

Q

Q

L

V

G

G

T

K

P

P

R

L

E

E

V

L

Y

Y

E

H

R

Y

P

P

A

A

N

D

L

R

F

F

T

V

A

A

R

G

F

F

V

I

G

G

-

S

-

P

E

K

T

M

N

N

F

F

F

L

P

P

G

V

T

K

V

V

E

T

S

A

V

T

Q

A

D

I

G

D

S

Q

I

V

K

Q

V

V

D

E

V

L

binding adenosine-5'-diphosphate: W12 (≠ F15), V17 (= V20), G38 (= G41), C39 (= C42), G40 (= G43), K41 (= K44), S42 (≠ T45), T43 (= T46), R128 (= R131), A132 (≠ Q135), S134 (= S137), Q137 (= Q140)
binding tetrafluoroaluminate ion: S37 (= S40), G38 (= G41), K41 (= K44), Q81 (= Q84), S134 (= S137), G135 (= G138), G136 (= G139), E158 (= E161), H191 (= H194)
binding magnesium ion: S42 (≠ T45), Q81 (= Q84)

3puvA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-vo4 (see paper)
44% identity, 68% coverage: 6:258/373 of query aligns to 3:257/371 of 3puvA

query
sites
3puvA

L

V

S

Q

L

L

S

Q

N

N

L

V

S

T

K

K

Q

A

F
x
W

G

G

G

E

K

V

T

V

V
|
V

L

S

D

K

G

D

L

I

D

N

L

L

E

D

I

I

Y

H

D

E

G

G

E

E

F

F

I

V

T

V

L

F

L

V

G

G

P

P

S

S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

R

R

L

M

M

I

A

A

G

G

F

L

E

E

H

T

P

I

D

T

E

S

G

G

T

D

I

L

T

F

L

I

A

G

G

E

E

K

N

R

L

M

T

N

H

D

T

T

A

P

P

P

E

A

N

E

R

R

P

G

L

V

N

G

T

M

V

V

F

F

Q
|
Q

H

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

L

S

S

V

V

F

A

D

E

N

N

V

M

A

S

Y

F

G

G

L

L

K

K

M

L

E

A

K

G

R

A

P

K

K

K

D

E

E

V

I

I

R

N

Q

Q

R

R

V

V

D

N

E

Q

A

V

L

A

A

E

M

V

V

L

Q

Q

L

L

Q

A

D

H

F

L

A

L

R

D

R
|
R

K

K

P

P

H

K

Q
x
A

L

L

S
|
S

G

G

Q
|
Q

Q

R

Q

Q

R

R

V

V

A

A

I

I

A

G

R

R

A

T

V

L

V

V

K

A

R

E

P

P

R

S

L

V

L

F

L

L

D

D

E

E

P

P

L

L

S

S

A

N

L

L

D

D

Y

A

K

A

L

L

R

R

R

V

T

Q

M

M

Q

R

V

I

E

E

L

I

K

S

R

R

L

L

Q

H

R

K

E

R

L

L

G

G

I

R

T

T

F

M

V

I

F

Y

V

V

T

T

H

H

D

D

Q

Q

E

V

E

E

A

A

L

M

S

T

M

L

S

A

D

D

R

K

V

I

V

V

L

L

K

D

D

A

G

G

L

R

V

V

Q

A

Q

Q

L

V

G

G

T

K

P

P

R

L

E

E

V

L

Y

Y

E

H

R

Y

P

P

A

A

N

D

L

R

F

F

T

V

A

A

R

G

F

F

V

I

G

G

-

S

-

P

E

K

T

M

N

N

F

F

F

L

P

P

G

V

T

K

V

V

E

T

S

A

V

T

Q

A

D

I

G

D

S

Q

I

V

K

Q

V

V

D

E

V

L

binding adenosine-5'-diphosphate: W12 (≠ F15), V17 (= V20), G38 (= G41), C39 (= C42), G40 (= G43), K41 (= K44), S42 (≠ T45), T43 (= T46), R128 (= R131), A132 (≠ Q135), S134 (= S137), Q137 (= Q140)
binding magnesium ion: S42 (≠ T45), Q81 (= Q84)

P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
44% identity, 68% coverage: 6:258/373 of query aligns to 4:258/371 of P68187

query
sites
P68187

L

V

S

Q

L

L

S

Q

N

N

L

V

S

T

K

K

Q

A

F

W

G

G

G

E

K

V

T

V

V

V

L

S

D

K

G

D

L

I

D

N

L

L

E

D

I

I

Y

H

D

E

G

G

E

E

F

F

I

V

T

V

L

F

L

V

G

G

P

P

S

S

G

G

C

C

G

G

K

K

T

S

T

T

L

L

R

R

L

M

M

I

A

A

G

G

F

L

E

E

H

T

P

I

D

T

E

S

G

G

T

D

I

L

T

F

L

I

A

G

G

E

E

K

N

R

L

M

T

N

H

D

T

T

A

P

P

P

E

A

N

E

R

R

P

G

L

V

N

G

T

M

V

V

F

F

Q

Q

H

S

Y

Y

A
|
A

L

L

F

Y

P

P

H

H

M

L

S

S

V

V

F

A

D

E

N

N

V

M

A

S

Y

F

G

G

L

L

K

K

M

L

E

A

K

G

R

A

P
x
K

K

K

D

E

E

V

I

I

R

N

Q

Q

R

R

V
|
V

D

N

E

Q

A
x
V

L

A

A
x
E

M

V

V

L

Q

Q

L

L

Q
x
A

D

H

F

L

A

L

R

D

R

R

K

K

P

P

H

K

Q

A

L

L

S

S

G

G

G
|
G

Q

Q

Q

R

Q

Q

R

R

V

V

A

A

I

I

A

G

R

R

A

T

V

L

V

V

K

A

R

E

P

P

R

S

L

V

L

F

L

L

D
|
D

E

E

P

P

L

L

S

S

A

N

L

L

D

D

Y

A

K

A

L

L

R

R

R

V

T

Q

M

M

Q

R

V

I

E

E

L

I

K

S

R

R

L

L

Q

H

R

K

E

R

L

L

G

G

I

R

T

T

F

M

V

I

F

Y

V

V

T

T

H

H

D

D

Q

Q

E

V

E

E

A

A

L

M

S

T

M

L

S

A

D

D

R

K

V

I

V

V

L

L

K

D

D

A

G

G

L

R

V

V

Q

A

Q

Q

L

V

G

G

T

K

P

P

R

L

E

E

V

L

Y

Y

E

H

R

Y

P

P

A

A

N

D

L
x
R

F

F

T

V

A

A

R

G

F

F

V

I

G

G

-

S

-

P

E

K

T

M

N

N

F
|
F

F

L

P

P

G

V

T

K

V

V

E

T

S

A

V

T

Q

A

D

I

G

D

S

Q

I

V

K

Q

V

V

D

E

V

L

A85 (= A87) mutation to M: Suppressor of EAA loop mutations in MalFG.
K106 (≠ P108) mutation to C: Suppressor of EAA loop mutations in MalFG.
V114 (= V116) mutation to C: Suppressor of EAA loop mutations in MalFG.
V117 (≠ A119) mutation to M: Suppressor of EAA loop mutations in MalFG.
E119 (≠ A121) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
A124 (≠ Q126) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
G137 (= G139) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
D158 (= D160) mutation to N: Loss of maltose transport but retains ability to repress mal genes.
R228 (≠ L230) mutation to C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
F241 (= F241) mutation to I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.

Sites not aligning to the query:

267 W→G: Normal maltose transport but constitutive mal gene expression.
278 G→P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
282 S→L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
284 G→S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
302 G→D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
308 E→Q: Maltose transport is affected but retains ability to interact with MalT.
322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
340 G→A: Maltose transport is affected but retains ability to interact with MalT.
346 G→S: Normal maltose transport but constitutive mal gene expression.
355 F→Y: Maltose transport is affected but retains ability to interact with MalT.

2awnC Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
43% identity, 66% coverage: 12:258/373 of query aligns to 2:227/344 of 2awnC

query
sites
2awnC

S

S

K

V

Q

Q

F

L

G

Q

G

N

K

V

T

T

V
|
V

L

S

D

K

G

D

L

I

D

N

L

L

E

D

I

I

Y

H

D

E

G

G

E

E

F

F

I

V

T

V

L

F

L

V

G

G

P

P

S

S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

R

R

L

M

M

I

A

A

G

G

F

L

E

G

H

-

P

-

D

-

E

-

G

-

T

-

I

-

T

-

L

-

A

-

G

-

E

-

N

-

L

-

T

-

H

-

T

-

A

-

P

-

E

-

N

-

R

-

P

-

L

V

N

G

T

M

V

V

F

F

Q

Q

H

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

L

S

S

V

V

F

A

D

E

N

N

V

M

A

S

Y

F

G

G

L

L

K

K

M

L

E

A

K

G

R

A

P

K

K

K

D

E

E

V

I

I

R

N

Q

Q

R

R

V

V

D

N

E

Q

A

V

L

A

A

E

M

V

V

L

Q

Q

L

L

Q

A

D

H

F

L

A

L

R

D

R

R

K

K

P

P

H

K

Q

A

L

L

S

S

G

G

Q

Q

Q

R

Q

Q

R

R

V

V

A

A

I

I

A

G

R

R

A

T

V

L

V

V

K

A

R

E

P

P

R

S

L

V

L

F

L

L

D

D

E

E

P

P

L

L

S

S

A

N

L

L

D

D

Y

A

K

A

L

L

R

R

R

V

T

Q

M

M

Q

R

V

I

E

E

L

I

K

S

R

R

L

L

Q

H

R

K

E

R

L

L

G

G

I

R

T

T

F

M

V

I

F

Y

V

V

T

T

H

H

D

D

Q

Q

E
&