SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF4143 PS417_21220 acetyl-CoA acetyltransferase to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4b3iD Crystal structure of mycobacterium tuberculosis fatty acid beta-oxidation complex with coenzymea bound at the hydratase active sites (see paper)
65% identity, 100% coverage: 3:401/401 of query aligns to 4:403/403 of 4b3iD

query
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4b3iD

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active site: C92 (= C91), H359 (= H357), C389 (= C387), G391 (= G389)
binding adenosine-5'-diphosphate: H243 (≠ S241), W244 (≠ H242)

O53871 Putative acyltransferase Rv0859; EC 2.3.1.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
65% identity, 100% coverage: 3:401/401 of query aligns to 4:403/403 of O53871

query
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O53871

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K189 (= K188) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

7o4tC Structure of mycobacterium tuberculosis beta-oxidation trifunctional enzyme with coenzyme a bound at the hydratase, thiolase active sites and possible additional binding site (coa(ech/had)) (see paper)
65% identity, 100% coverage: 3:401/401 of query aligns to 3:402/402 of 7o4tC

query
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7o4tC

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binding coenzyme a: C91 (= C91), M126 (= M126), Q148 (= Q148), R209 (= R209), T212 (≠ S212), L217 (= L217), L220 (= L220), A223 (≠ S223), F224 (= F224), T252 (= T251), G253 (≠ P252), G254 (= G253), S256 (= S255), S257 (= S256), I259 (= I258), F329 (= F328), H358 (= H357)
binding 3'-phosphate-adenosine-5'-diphosphate: H242 (≠ S241), W243 (≠ H242)

4b3jC Crystal structure of mycobacterium tuberculosis fatty acid beta-oxidation complex with coenzymea bound at the hydratase and thiolase active sites (see paper)
65% identity, 100% coverage: 3:401/401 of query aligns to 3:402/402 of 4b3jC

query
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4b3jC

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K

R

T

A

A

A

A

V

L

L

V

A

A

S

D

G

W

M

D

P

I

V

S

T

V

S

A

G

G

G

V

V

Q

Q

I

L

N

N

R

R

F

F

C
|
C

A

A

S

S

G

G

L

L

E

E

A

A

V

V

N

N

V

T

G

A

A

A

M

Q

K

K

V

V

R

R

S

S

G

G

F

W

E

D

D

D

L

L

V

V

V

L

V

A

G

G

V

V

E

E

S

S

M

M

S

S

R

R

V

V

P

P

M
|
M

G

G

S

S

D

D

G

G

A

A

W

M

V

G

L

L

D

D

P

P

Q

A

T

T

N

N

M

Y

H

D

S

V

H

M

F

F

T

V

P

P

Q
|
Q

G

S

I

I

G

G

A

A

D

D

L

L

I

I

A

A

T

T

L

I

E

E

G

G

F

F

T

S

R

R

D

E

D

D

V

V

D

D

A

A

F

Y

A

A

L

L

H

R

S

S

Q

Q

K

K

A

A

R

E

A

A

R

W

A

S

D

G

G

G

S

Y

F

F

N

A

K

K

S

S

L

V

I

V

A

P

V

V

Q

R

D

D

Q

Q

N

N

G

G

I

L

V

L

L

I

L

L

D

D

H

H

D

D

E

E

F

H

I

M

R
|
R

G

P

D

D

S
x
T

T

T

L

K

E

E

G

G

L
|
L

G

A

K

K

L
|
L

K

K

P

P

S

A

F
|
F

E

E

M

G

M

L

G

A

Q

A

M

L

-

G

G

G

F

F

D

D

A

D

T

V

A

A

L

L

R

Q

V

K

Y

Y

S
x
H

H
x
W

V

V

E

E

R

K

I

I

H

N

H

H

V

V

H

H

T
|
T

P
x
G

G
|
G

N

N

S
|
S

G

G

I
|
I

V

V

D

D

G

G

A

A

L

L

M

V

L

M

I

I

G

G

S

S

E

A

A

A

K

A

G

G

R

K

E

L

L

Q

G

G

L

L

Q

T

P

P

R

R

A

A

R

R

I

I

V

V

A

A

T

T

A

A

V

T

T

S

S

G

T

A

D

D

P

P

T

V

I

I

M

M

L

L

T

T

G

G

P

P

A

T

P

P

A

A

T

T

R

R

K

K

A

V

L

L

A

D

K

R

A

A

G

G

L

L

R

T

I

V

E

D

D

D

I

I

D

D

L

L

F

F

E

E

V

L

N

N

E

E

A

A

F
|
F

A

A

S

S

V

V

L

L

K

K

F

F

I

Q

K

K

D

D

M

L

G

N

I

I

D

P

A

D

A

E

R

K

V

L

N

N

V

V

N

N

G

G

S

A

I

I

A

A

M

M

G

G

H
|
H

P

P

L
|
L

G

G

A

A

T

T

G

G

C

A

A

M

I

I

L

L

G

G

T

T

L

M

L

V

D

D

E

E

L

L

E

E

V

R

R

R

Q

N

Q

A

R

R

Y

R

G

A

L

L

A

I

T

T

L

L

C
|
C

V

I

G
|
G

G

G

M

M

G

G

I

V

A

A

T

T

I

I

E

E

R

R

L

V

active site: C91 (= C91), H358 (= H357), C388 (= C387), G390 (= G389)
binding adenosine-5'-diphosphate: H242 (≠ S241), W243 (≠ H242)
binding coenzyme a: C91 (= C91), M126 (= M126), Q148 (= Q148), R209 (= R209), T212 (≠ S212), L217 (= L217), L220 (= L220), F224 (= F224), T252 (= T251), G253 (≠ P252), G254 (= G253), S256 (= S255), S257 (= S256), I259 (= I258), F329 (= F328), H358 (= H357), L360 (= L359)

2d3tC Fatty acid beta-oxidation multienzyme complex from pseudomonas fragi, form v (see paper)
37% identity, 99% coverage: 5:401/401 of query aligns to 8:390/390 of 2d3tC

query
sites
2d3tC

L

V

I

I

F

V

D

D

A

F

I

G

R

R

T

T

P

P

R

M

G

G

K

R

G

S

K

K

A

-

D

-

G

G

A

G

L

M

H

H

-

R

S

N

V

T

K

R

P

A

V

E

N

D

L

M

V

S

A

A

G

H

L

L

L

I

T

S

-

K

A

V

L

L

A

E

R

R

R

N

S

S

D

K

L

V

D

D

T

P

H

G

Q

E

V

V

D

E

D

D

I

V

V

I

L

W

G

G

C

C

V

V

T

N

P

Q

I

T

G

L

D

E

Q

Q

G

G

A

W

D

N

I

I

A

A

K

R

T

M

A

A

A

S

L

L

V

M

A

T

D

Q

W

I

D

P

I

H

S

T

V

S

A

A

G

A

V

Q

Q

T

I

V

N

S

R

R

F

L

C
|
C

A

G

S

S

G

S

L

M

E

S

A

A

V

L

N

H

V

T

G

A

A

A

M

Q

K

A

V

I

R

M

S

T

G

G

F

N

E

G

D

D

L

V

V

F

V

V

G

G

V

V

E

E

S

H

M

M

S

G

R

H

V

V

P

S

M
|
M

G

M

S

H

D

G

G

-

A

-

W

-

V

-

L

V

D

D

P

P

Q

N

T

P

N

H

M

M

H

S

S

L

H

Y

F

A

T

A

P

K

-

A

-

S

-

G

-

M

Q
x
M

G

G

I

L

G

T

A

A

D

E

L

M

I

L

A

G

T

K

L

M

E

H

G

G

F

I

T

S

R

R

D

E

D

Q

V

Q

D

D

A

A

F

F

A

A

L

V

H

R

S

S

Q
x
H

Q

Q

K

L

A

A

A

H

R

K

A

A

R

T

A

V

D

E

G

G

S

K

F

F

N

K

K

D

S

E

L

I

I

I

A

P

V

M

Q

Q

-

G

-

Y

D

D

Q

E

N

N

G

G

I

F

V

L

-

K

L

I

L

F

D

D

H

Y

D

D

E

E

F

T

I

I

R
|
R

G

P

D

D

S

T

T

T

L

L

E

E

G

S

L
|
L

G

A

K

A

L
|
L

K

K

P

P

S

A

F

F

E

N

M

P

M

K

G

G

Q

G

M

-

G

-

F

-

D

-

A

-

T

-

A

-

L

-

R

-

V

-

Y

-

S

-

H

-

V

-

E

-

R

-

I

-

H

-

V

T

H

V

T

T

P
x
A

G
|
G

N

T

S

S

S
|
S

G

Q

I
|
I

V

T

D

D

G

G

A

A

A

S

L

C

M

M

L

I

I

V

G

M

S

S

E

A

A

Q

K

R

G

A

R

K

E

D

L

L

G

G

L

L

Q

E

P

P

R

L

A

A

R

V

I

I

V

R

A

S

T

M

A

A

V

V

T

A

S

G

T

V

D

D

P

P

T

A

I

I

M
|
M

L

G

T

Y

G

G

P

P

A

V

P

P

A

A

T

T

R

Q

K

K

A

A

L

L

A

K

K

R

A

A

G

G

L

L

R

N

I

M

E

A

D

D

I

I

D

D

L

F

F

I

E

E

V

L

N

N

E

E

A
|
A

F
|
F

A

A

S

A

V

Q

V

A

L

L

K

P

F

V

I

L

K

K

D

D

M

L

G

K

I

V

-

L

-

D

-

K

D

M

A

N

A

E

R

K

V

V

N

N

V

L

N

H

G

G

S

A

I

I

A

A

M

L

G

G

H
|
H

P

P

L

F

G

G

A

C

T

S

G

G

C

A

A

R

I

I

L

S

G

G

T

T

L

L

D

N

E

V

L

M

E

K

V

Q

R

N

Q

G

R

T

Y

F

G

G

L

L

A

S

T

T

L

M

C
|
C

V

I

G
|
G

G

L

G

G

M

Q

G

G

I

I

A

A

T

T

I

V

I

F

E

E

R

R

L

V

active site: C94 (= C91), H346 (= H357), C376 (= C387), G378 (= G389)
binding acetyl coenzyme *a: C94 (= C91), M129 (= M126), M150 (≠ Q148), H176 (≠ Q174), R214 (= R209), L222 (= L217), L225 (= L220), A238 (≠ P252), G239 (= G253), S242 (= S256), I244 (= I258), M283 (= M297), A313 (= A327), F314 (= F328), H346 (= H357), C376 (= C387)

P07097 Acetyl-CoA acetyltransferase; Acetoacetyl-CoA thiolase; Beta-ketothiolase; EC 2.3.1.9 from Shinella zoogloeoides (Crabtreella saccharophila) (see 2 papers)
38% identity, 100% coverage: 2:401/401 of query aligns to 3:392/392 of P07097

query
sites
P07097

T

T

Q

P

A

S

L

I

I

V

F

I

D

A

A

S

I

A

R

R

T

T

P

-

R

-

G

A

K

V

G

G

K

S

A

F

D

N

G

G

A

A

L

F

H

A

S

N

V

T

K

P

P

A

V

H

N

E

L

L

V

G

A

A

G

T

L

V

L

I

T

S

A

A

L

V

A

L

R

E

R

R

S

A

D

G

L

V

D

A

T

A

H

G

Q

E

V

V

D

N

D

E

I

V

V

I

L

L

G

G

C

Q

V

V

T

L

P

P

I

A

G

G

D

-

Q

E

G

G

A
x
Q

D

N

I

P

A

A

K

R

T

Q

A

A

L

M

V

K

A

A

D

G

W

V

D

P

I

Q

S

E

V

A

A

T

G

A

V

W

Q

G

I

M

N

N

R

Q

F

L

C
|
C

A

G

S

S

G

G

L

L

E

R

A

A

V

V

N

A

V

L

G

G

A

M

M

Q

K

Q

V

I

R

A

S

T

G

G

F

D

E

A

D

S

L

I

V

I

V

V

V

A

G

G

V

M

E

E

S

S

M

M

S

S

R

M

V

A

P

P

M

H

G

C

S

A

D

H

-

L

-

A

G

G

A

V

W

K

V

M

L

G

D

D

P

F

Q

K

-

M

-

I

-

D

T

T

N

M

M

I

H

K

S

D

H

G

F

L

T

T

P

D

-

A

-

F

-

Y

-

G

-

Y

-

H

Q

M

G

G

I

T

G

T

A

A

D

E

L

N

I

V

A

A

T

K

L

Q

E

W

G

Q

F

L

T

S

R

R

D

D

D

E

V

Q

D

D

A

A

F

F

A

A

L

V

H

A

S

S

Q

Q

Q

N

K

K

A

A

A

E

R

A

A

A

R

Q

A

K

D

D

G

G

S

R

F

F

N

K

K

D

S

E

L

I

I

V

A

P

-

F

-

I

V

V

Q

K

D

G

Q

R

N

K

G

G

I

D

V

I

L

T

L

V

D

D

H

A

D

D

E

E

F

Y

I

I

R

R

G

H

D

G

S

A

T

T

L

L

E

D

G

S

L

M

G

A

K

K

L

L

K

R

P

P

S

A

F

F

E

D

M

K

M

E

G

G

Q

T

M

V

G

-

F

-

D

-

A

-

T

-

A

-

L

-

R

-

V

-

Y

-

S

-

H

-

V

-

E

-

R

-

I

-

H

-

V

-

H

-

T

T

P

A

G

G

N

N

S

A

S

S

G

G

I

L

V

N

D

D

G

G

A

A

L

A

M

A

L

L

I

L

G

M

S

S

E

E

A

A

K

E

G

A

R

S

E

R

L

R

G

G

L

I

Q

Q

P

P

R

L

A

G

R

R

I

I

V

V

A

S

T

W

A

A

V

T

T

V

S

G

T

V

D

D

P

P

T

K

I

V

M

M

L

G

T

T

G

G

P

P

A

I

P

P

A

A

T

S

R

R

K

K

A

A

L

L

A

E

K

R

A

A

G

G

L

W

R

K

I

I

E

G

D

D

I

L

D

D

L

L

F

V

E

E

V

A

N

N

E

E

A

A

F

F

A

A

S

A

V

Q

V

A

L

C

K

A

F

V

I

N

K

K

D

D

M

L

G

G

I

W

D

D

A

P

A

S

R

I

V

V

N

N

V

V

N

N

G

G

S

A

I

I

A

A

M

I

G

G

H

H

P

P

L

I

G

G

A

A

T

S

G

G

C

A

A

R

I

I

L

L

G

N

T

T

L

L

D

F

E

E

L

M

E

K

V

R

R

R

Q

G

Q

A

R

R

Y

K

G

G

L

L

A

A

T

T

L

L

C
|
C

V

I

G

G

M

M

G

G

I

V

A

A

T

M

I

C

I

I

E

E

R

S

L

L

Q64 (≠ A66) mutation to A: Slightly lower activity.
C89 (= C91) mutation to A: Loss of activity.
C378 (= C387) mutation to G: Loss of activity.

1ou6A Biosynthetic thiolase from zoogloea ramigera in complex with acetyl-o- pantetheine-11-pivalate
39% identity, 99% coverage: 5:401/401 of query aligns to 6:392/392 of 1ou6A

query
sites
1ou6A

L

V

I

I

F

A

D

S

A

A

I

A

R

R

T

T

P

-

R

-

G

A

K

V

G

G

K

S

A

F

D

N

G

G

A

A

L

F

H

A

S

N

V

T

K

P

P

A

V

H

N

E

L

L

V

G

A

A

G

T

L

V

L

I

T

S

A

A

L

V

A

L

R

E

R

R

S

A

D

G

L

V

D

A

T

A

H

G

Q

E

V

V

D

N

D

E

I

V

V

I

L

L

G

G

C

Q

V

V

T

L

P

P

I

A

G

G

D

-

Q

E

G

G

A

Q

D

N

I

P

A

A

K

R

T

Q

A

A

L

M

V

K

A

A

D

G

W

V

D

P

I

Q

S

E

V

A

A

T

G

A

V

W

Q

G

I

M

N

N

R

Q

F

L

C
|
C

A

G

S

S

G

G

L

L

E

R

A

A

V

V

N

A

V

L

G

G

A

M

M

Q

K

Q

V

I

R

A

S

T

G

G

F

D

E

A

D

S

L

I

V

I

V

V

V

A

G

G

V

M

E

E

S

S

M

M

S

S

R

M

V

A

P

P

M

H

G

C

S

A

D

H

-

L

-

R

G

G

A

V

W

K

V

M

L

G

D

D

P

F

Q

K

-

M

-

I

-

D

T

T

N

M

M
x
I

H

K

S

D

H

G

F
x
L

T

T

P

D

-

A

-

F

-

Y

-

G

-

Y

-
x
H

Q
x
M

G

G

I

T

G

T

A

A

D

E

L

N

I

V

A

A

T

K

L

Q

E

W

G

Q

F

L

T

S

R

R

D

D

D

E

V

Q

D

D

A

A

F

F

A

A

L

V

H

A

S

S

Q

Q

Q

N

K

K

A

A

A

E

R

A

A

A

R

Q

A

K

D

D

G

G

S

R

F

F

N

K

K

D

S

E

L

I

I

V

A

P

-

F

-

I

V

V

Q

K

D

G

Q

R

N

K

G

G

I

D

V

I

L

T

L

V

D

D

H

A

D

D

E

E

F

Y

I

I

R

R

G

H

D

G

S

A

T

T

L

L

E

D

G

S

L

M

G

A

K

K

L

L

K

R

P

P

S
x
A

F
|
F

E

D

M

K

M

E

G

G

Q

T

M

V

G

-

F

-

D

-

A

-

T

-

A

-

L

-

R

-

V

-

Y

-

S

-

H

-

V

-

E

-

R

-

I

-

H

-

V

-

H

-

T

T

P
x
A

G

G

N

N

S

A

S
|
S

G
|
G

I
x
L

V

N

D

D

G

G

A

A

L

A

M

A

L

L

I

L

G

M

S

S

E

E

A

A

K

E

G

A

R

S

E

R

L

R

G

G

L

I

Q

Q

P

P

R

L

A

G

R

R

I

I

V

V

A

S

T

W

A

A

V

T

T

V

S

G

T

V

D

D

P

P

T

K

I

V

M

M

L

G

T

T

G

G

P

P

A

I

P

P

A

A

T

S

R

R

K

K

A

A

L

L

A

E

K

R

A

A

G

G

L

W

R

K

I

I

E

G

D

D

I

L

D

D

L

L

F

V

E

E

V

A

N

N

E

E

A
|
A

F
|
F

A

A

S

A

V

Q

V

A

L

C

K

A

F

V

I

N

K

K

D

D

M

L

G

G

I

W

D

D

A

P

A

S

R

I

V

V

N

N

V

V

N

N

G

G

S

A

I

I

A

A

M

I

G

G

H
|
H

P

P

L

I

G

G

A

A

T

S

G

G

C

A

A

R

I

I

L

L

G

N

T

T

L

L

D

F

E

E

L

M

E

K

V

R

R

R

Q

G

Q

A

R

R

Y

K

G

G

L

L

A

A

T

T

L

L

C
|
C

V

I

G
|
G

G

G

M

M

G

G

I

V

A

A

T

M

I

C

I

I

E

E

R

S

L

L

active site: C89 (= C91), H348 (= H357), C378 (= C387), G380 (= G389)
binding pantothenyl-aminoethanol-acetate pivalic acid: I144 (≠ M141), L148 (≠ F145), H156 (vs. gap), M157 (≠ Q148), A234 (≠ S223), F235 (= F224), A243 (≠ P252), S247 (= S256), G248 (= G257), L249 (≠ I258), A318 (= A327), F319 (= F328), H348 (= H357)

2vu2A Biosynthetic thiolase from z. Ramigera. Complex with s-pantetheine-11- pivalate. (see paper)
39% identity, 99% coverage: 5:401/401 of query aligns to 3:389/389 of 2vu2A

query
sites
2vu2A

L

V

I

I

F

A

D

S

A

A

I

A

R

R

T

T

P

-

R

-

G

A

K

V

G

G

K

S

A

F

D

N

G

G

A

A

L

F

H

A

S

N

V

T

K

P

P

A

V

H

N

E

L

L

V

G

A

A

G

T

L

V

L

I

T

S

A

A

L

V

A

L

R

E

R

R

S

A

D

G

L

V

D

A

T

A

H

G

Q

E

V

V

D

N

D

E

I

V

V

I

L

L

G

G

C

Q

V

V

T

L

P

P

I

A

G

G

D

-

Q

E

G

G

A

Q

D

N

I

P

A

A

K

R

T

Q

A

A

L

M

V

K

A

A

D

G

W

V

D

P

I

Q

S

E

V

A

A

T

G

A

V

W

Q

G

I

M

N

N

R

Q

F

L

C
|
C

A

G

S

S

G

G

L

L

E

R

A

A

V

V

N

A

V

L

G

G

A

M

M

Q

K

Q

V

I

R

A

S

T

G

G

F

D

E

A

D

S

L

I

V

I

V

V

V

A

G

G

V

M

E

E

S

S

M

M

S

S

R

M

V

A

P

P

M

H

G

C

S

A

D

H

-

L

-

R

G

G

A

V

W

K

V

M

L

G

D

D

P

F

Q

K

-

M

-

I

-

D

T

T

N

M

M

I

H

K

S

D

H

G

F
x
L

T

T

P

D

-

A

-

F

-

Y

-

G

-

Y

-
x
H

Q
x
M

G

G

I

T

G

T

A

A

D

E

L

N

I

V

A

A

T

K

L

Q

E

W

G

Q

F

L

T

S

R

R

D

D

D

E

V

Q

D

D

A

A

F

F

A

A

L

V

H

A

S

S

Q

Q

Q

N

K

K

A

A

A

E

R

A

A

A

R

Q

A

K

D

D

G

G

S

R

F

F

N

K

K

D

S

E

L

I

I

V

A

P

-

F

-

I

V

V

Q

K

D

G

Q

R

N

K

G

G

I

D

V

I

L

T

L

V

D

D

H

A

D

D

E

E

F

Y

I

I

R

R

G

H

D

G

S

A

T

T

L

L

E

D

G

S

L

M

G

A

K

K

L

L

K

R

P

P

S

A

F
|
F

E

D

M

K

M

E

G

G

Q

T

M

V

G

-

F

-

D

-

A

-

T

-

A

-

L

-

R

-

V

-

Y

-

S

-

H

-

V

-

E

-

R

-

I

-

H

-

V

-

H

-

T

T

P
x
A

G

G

N

N

S

A

S
|
S

G
|
G

I
x
L

V

N

D

D

G

G

A

A

L

A

M

A

L

L

I

L

G

M

S

S

E

E

A

A

K

E

G

A

R

S

E

R

L

R

G

G

L

I

Q

Q

P

P

R

L

A

G

R

R

I

I

V

V

A

S

T

W

A

A

V

T

T

V

S

G

T

V

D

D

P

P

T

K

I

V

M

M

L

G

T

T

G

G

P

P

A

I

P

P

A

A

T

S

R

R

K

K

A

A

L

L

A

E

K

R

A

A

G

G

L

W

R

K

I

I

E

G

D

D

I

L

D

D

L

L

F

V

E

E

V

A

N

N

E

E

A
|
A

F
|
F

A

A

S

A

V

Q

V

A

L

C

K

A

F

V

I

N

K

K

D

D

M

L

G

G

I

W

D

D

A

P

A

S

R

I

V

V

N

N

V

V

N

N

G

G

S

A

I

I

A

A

M

I

G

G

H
|
H

P

P

L

I

G

G

A

A

T

S

G

G

C

A

A

R

I

I

L

L

G

N

T

T

L

L

D

F

E

E

L

M

E

K

V

R

R

R

Q

G

Q

A

R

R

Y

K

G

G

L

L

A

A

T

T

L

L

C
|
C

V

I

G
|
G

G

G

M

M

G

G

I

V

A

A

T

M

I

C

I

I

E

E

R

S

L

L

active site: C86 (= C91), H345 (= H357), C375 (= C387), G377 (= G389)
binding (3R)-3-hydroxy-2,2-dimethyl-4-oxo-4-({3-oxo-3-[(2-sulfanylethyl)amino]propyl}amino)butyl 2,2-dimethylpropanoate: C86 (= C91), L145 (≠ F145), H153 (vs. gap), M154 (≠ Q148), F232 (= F224), A240 (≠ P252), S244 (= S256), G245 (= G257), L246 (≠ I258), A315 (= A327), F316 (= F328), H345 (= H357)

1dm3A Acetylated biosynthetic thiolase from zoogloea ramigera in complex with acetyl-coa (see paper)
39% identity, 99% coverage: 5:401/401 of query aligns to 3:389/389 of 1dm3A

query
sites
1dm3A

L

V

I

I

F

A

D

S

A

A

I

A

R

R

T

T

P

-

R

-

G

A

K

V

G

G

K

S

A

F

D

N

G

G

A

A

L

F

H

A

S

N

V

T

K

P

P

A

V

H

N

E

L

L

V

G

A

A

G

T

L

V

L

I

T

S

A

A

L

V

A

L

R

E

R

R

S

A

D

G

L

V

D

A

T

A

H

G

Q

E

V

V

D

N

D

E

I

V

V

I

L

L

G

G

C

Q

V

V

T

L

P

P

I

A

G

G

D

-

Q

E

G

G

A

Q

D

N

I

P

A

A

K

R

T

Q

A

A

L

M

V

K

A

A

D

G

W

V

D

P

I

Q

S

E

V

A

A

T

G

A

V

W

Q

G

I

M

N

N

R

Q

F

L

C
|
C

A

G

S

S

G

G

L

L

E

R

A

A

V

V

N

A

V

L

G

G

A

M

M

Q

K

Q

V

I

R

A

S

T

G

G

F

D

E

A

D

S

L

I

V

I

V

V

V

A

G

G

V

M

E

E

S

S

M

M

S

S

R

M

V

A

P

P

M

H

G

C

S

A

D

H

-

L

-

R

G

G

A

V

W

K

V

M

L

G

D

D

P

F

Q

K

-

M

-

I

-

D

T

T

N

M

M

I

H

K

S

D

H

G

F
x
L

T

T

P

D

-

A

-

F

-

Y

-

G

-

Y

-
x
H

Q
x
M

G

G

I

T

G

T

A

A

D

E

L

N

I

V

A

A

T

K

L

Q

E

W

G

Q

F

L

T

S

R

R

D

D

D

E

V

Q

D

D

A

A

F

F

A

A

L

V

H

A

S

S

Q

Q

Q

N

K

K

A

A

A

E

R

A

A

A

R

Q

A

K

D

D

G

G

S

R

F

F

N

K

K

D

S

E

L

I

I

V

A

P

-

F

-

I

V

V

Q

K

D

G

Q

R

N

K

G

G

I

D

V

I

L

T

L

V

D

D

H

A

D

D

E

E

F

Y

I

I

R
|
R

G

H

D

G

S

A

T

T

L

L

E

D

G
x
S

L
x
M

G

A

K

K

L
|
L

K

R

P

P

S

A

F
|
F

E

D

M

K

M

E

G

G

Q

T

M

V

G

-

F

-

D

-

A

-

T

-

A

-

L

-

R

-

V

-

Y

-

S

-

H

-

V

-

E

-

R

-

I

-

H

-

V

-

H

-

T

T

P
x
A

G
|
G

N

N

S
x
A

S
|
S

G
|
G

I
x
L

V

N

D

D

G

G

A

A

L

A

M

A

L

L

I

L

G

M

S

S

E

E

A

A

K

E

G

A

R

S

E

R

L

R

G

G

L

I

Q

Q

P

P

R

L

A

G

R

R

I

I

V

V

A

S

T

W

A

A

V

T

T

V

S

G

T

V

D

D

P

P

T

K

I

V

M
|
M

L

G

T

T

G

G

P

P

A

I

P

P

A

A

T

S

R

R

K

K

A

A

L

L

A

E

K

R

A

A

G

G

L

W

R

K

I

I

E

G

D

D

I

L

D

D

L

L

F

V

E

E

V

A

N

N

E

E

A
|
A

F
|
F

A

A

S

A

V

Q

V

A

L

C

K

A

F

V

I

N

K

K

D

D

M

L

G

G

I

W

D

D

A

P

A

S

R

I

V

V

N

N

V

V

N

N

G

G

S

A

I

I

A

A

M

I

G

G

H
|
H

P

P

L

I

G

G

A

A

T

S

G

G

C

A

A

R

I

I

L

L

G

N

T

T

L

L

D

F

E

E

L

M

E

K

V

R

R

R

Q

G

Q

A

R

R

Y

K

G

G

L

L

A

A

T

T

L

L

C
|
C

V
x
I

G
|
G

G

G

M

M

G

G

I

V

A

A

T

M

I

C

I

I

E

E

R

S

L

L

active site: C86 (= C91), H345 (= H357), C375 (= C387), G377 (= G389)
binding acetyl coenzyme *a: C86 (= C91), L145 (≠ F145), H153 (vs. gap), M154 (≠ Q148), R217 (= R209), S224 (≠ G216), M225 (≠ L217), L228 (= L220), F232 (= F224), A240 (≠ P252), G241 (= G253), A243 (≠ S255), S244 (= S256), G245 (= G257), L246 (≠ I258), M285 (= M297), A315 (= A327), F316 (= F328), H345 (= H357), C375 (= C387), I376 (≠ V388)

1dlvA Biosynthetic thiolase from zoogloea ramigera in complex with coa (see paper)
39% identity, 99% coverage: 5:401/401 of query aligns to 3:389/389 of 1dlvA

query
sites
1dlvA

L

V

I

I

F

A

D

S

A

A

I

A

R

R

T

T

P

-

R

-

G

A

K

V

G

G

K

S

A

F

D

N

G

G

A

A

L

F

H

A

S

N

V

T

K

P

P

A

V

H

N

E

L

L

V

G

A

A

G

T

L

V

L

I

T

S

A

A

L

V

A

L

R

E

R

R

S

A

D

G

L

V

D

A

T

A

H

G

Q

E

V

V

D

N

D

E

I

V

V

I

L

L

G

G

C

Q

V

V

T

L

P

P

I

A

G

G

D

-

Q

E

G

G

A

Q

D

N

I

P

A

A

K

R

T

Q

A

A

L

M

V

K

A

A

D

G

W

V

D

P

I

Q

S

E

V

A

A

T

G

A

V

W

Q

G

I

M

N

N

R

Q

F

L

C
|
C

A

G

S

S

G

G

L

L

E

R

A

A

V

V

N

A

V

L

G

G

A

M

M

Q

K

Q

V

I

R

A

S

T

G

G

F

D

E

A

D

S

L

I

V

I

V

V

V

A

G

G

V

M

E

E

S

S

M

M

S

S

R

M

V

A

P

P

M

H

G

C

S

A

D

H

-

L

-

R

G

G

A

V

W

K

V

M

L

G

D

D

P

F

Q

K

-

M

-

I

-

D

T

T

N

M

M

I

H

K

S

D

H

G

F
x
L

T

T

P

D

-

A

-

F

-

Y

-

G

-

Y

-
x
H

Q
x
M

G

G

I

T

G

T

A

A

D

E

L

N

I

V

A

A

T

K

L

Q

E

W

G

Q

F

L

T

S

R

R

D

D

D

E

V

Q

D

D

A

A

F

F

A

A

L

V

H

A

S

S

Q

Q

Q

N

K

K

A

A

A

E

R

A

A

A

R

Q

A

K

D

D

G

G

S

R

F

F

N

K

K

D

S

E

L

I

I

V

A

P

-

F

-

I

V

V

Q

K

D

G

Q

R

N

K

G

G

I

D

V

I

L

T

L

V

D

D

H

A

D

D

E

E

F

Y

I

I

R
|
R

G

H

D

G

S

A

T

T

L

L

E

D

G
x
S

L
x
M

G

A

K

K

L
|
L

K

R

P

P

S

A

F
|
F

E

D

M

K

M

E

G

G

Q

T

M

V

G

-

F

-

D

-

A

-

T

-

A

-

L

-

R

-

V

-

Y

-

S

-

H

-

V

-

E

-

R

-

I

-

H

-

V

-

H

-

T

T

P
x
A

G
|
G

N

N

S

A

S
|
S

G
|
G

I
x
L

V

N

D

D

G

G

A

A

L

A

M

A

L

L

I

L

G

M

S

S

E

E

A

A

K

E

G

A

R

S

E

R

L

R

G

G

L

I

Q

Q

P

P

R

L

A

G

R

R

I

I

V

V

A

S

T

W

A

A

V

T

T

V

S

G

T

V

D

D

P

P

T

K

I

V

M

M

L

G

T

T

G

G

P

P

A

I

P

P

A

A

T

S

R

R

K

K

A

A

L

L

A

E

K

R

A

A

G

G

L

W

R

K

I

I

E

G

D

D

I

L

D

D

L

L

F

V

E

E

V

A

N

N

E

E

A

A

F
|
F

A

A

S

A

V

Q

V

A

L

C

K

A

F

V

I

N

K

K

D

D

M

L

G

G

I

W

D

D

A

P

A

S

R

I

V

V

N

N

V

V

N

N

G

G

S

A

I

I

A

A

M

I

G

G

H
|
H

P

P

L

I

G

G

A

A

T

S

G

G

C

A

A

R

I

I

L

L

G

N

T

T

L

L

D

F

E

E

L

M

E

K

V

R

R

R

Q

G

Q

A

R

R

Y

K

G

G

L

L

A

A

T

T

L

L

C
|
C

V

I

G
|
G

G

G

M

M

G

G

I

V

A

A

T

M

I

C

I

I

E

E

R

S

L

L

active site: C86 (= C91), H345 (= H357), C375 (= C387), G377 (= G389)
binding coenzyme a: C86 (= C91), L145 (≠ F145), H153 (vs. gap), M154 (≠ Q148), R217 (= R209), S224 (≠ G216), M225 (≠ L217), L228 (= L220), F232 (= F224), A240 (≠ P252), G241 (= G253), S244 (= S256), G245 (= G257), L246 (≠ I258), F316 (= F328), H345 (= H357)

2vu1A Biosynthetic thiolase from z. Ramigera. Complex of with o-pantheteine- 11-pivalate. (see paper)
39% identity, 99% coverage: 5:401/401 of query aligns to 5:391/391 of 2vu1A

query
sites
2vu1A

L

V

I

I

F

A

D

S

A

A

I

A

R

R

T

T

P

-

R

-

G

A

K

V

G

G

K

S

A

F

D

N

G

G

A

A

L

F

H

A

S

N

V

T

K

P

P

A

V

H

N

E

L

L

V

G

A

A

G

T

L

V

L

I

T

S

A

A

L

V

A

L

R

E

R

R

S

A

D

G

L

V

D

A

T

A

H

G

Q

E

V

V

D

N

D

E

I

V

V

I

L

L

G

G

C

Q

V

V

T

L

P

P

I

A

G

G

D

-

Q

E

G

G

A

Q

D

N

I

P

A

A

K

R

T

Q

A

A

L

M

V

K

A

A

D

G

W

V

D

P

I

Q

S

E

V

A

A

T

G

A

V

W

Q

G

I

M

N

N

R

Q

F

L

C
|
C

A

G

S

S

G

G

L

L

E

R

A

A

V

V

N

A

V

L

G

G

A

M

M

Q

K

Q

V

I

R

A

S

T

G

G

F

D

E

A

D

S

L

I

V

I

V

V

V

A

G

G

V

M

E

E

S

S

M

M

S

S

R

M

V

A

P

P

M

H

G

C

S

A

D

H

-

L

-

R

G

G

A

V

W

K

V

M

L

G

D

D

P

F

Q

K

-

M

-

I

-

D

T

T

N

M

M

I

H

K

S

D

H

G

F
x
L

T

T

P

D

-

A

-

F

-

Y

-

G

-

Y

-
x
H

Q

M

G

G

I

T

G

T

A

A

D

E

L

N

I

V

A

A

T

K

L

Q

E

W

G

Q

F

L

T

S

R

R

D

D

D

E

V

Q

D

D

A

A

F

F

A

A

L

V

H

A

S

S

Q

Q

Q

N

K

K

A

A

A

E

R

A

A

A

R

Q

A

K

D

D

G

G

S

R

F

F

N

K

K

D

S

E

L

I

I

V

A

P

-

F

-

I

V

V

Q

K

D

G

Q

R

N

K

G

G

I

D

V

I

L

T

L

V

D

D

H

A

D

D

E

E

F

Y

I

I

R

R

G

H

D

G

S

A

T

T

L

L

E

D

G

S

L

M

G

A

K

K

L

L

K

R

P

P

S

A

F
|
F

E

D

M

K

M

E

G

G

Q

T

M

V

G

-

F

-

D

-

A

-

T

-

A

-

L

-

R

-

V

-

Y

-

S

-

H

-

V

-

E

-

R

-

I

-

H

-

V

-

H

-

T

T

P
x
A

G

G

N

N

S

A

S
|
S

G
|
G

I
x
L

V

N

D

D

G

G

A

A

L

A

M

A

L

L

I

L

G

M

S

S

E

E

A

A

K

E

G

A

R

S

E

R

L

R

G

G

L

I

Q

Q

P

P

R

L

A

G

R

R

I

I

V

V

A

S

T

W

A

A

V

T

T

V

S

G

T

V

D

D

P

P

T

K

I

V

M

M

L

G

T

T

G

G

P

P

A

I

P

P

A

A

T

S

R

R

K

K

A

A

L

L

A

E

K

R

A

A

G

G

L

W

R

K

I

I

E

G

D

D

I

L

D

D

L

L

F

V

E

E

V

A

N

N

E

E

A
|
A

F
|
F

A

A

S

A

V

Q

V

A

L

C

K

A

F

V

I

N

K

K

D

D

M

L

G

G

I

W

D

D

A

P

A

S

R

I

V

V

N

N

V

V

N

N

G

G

S

A

I

I

A

A

M

I

G

G

H
|
H

P

P

L

I

G

G

A

A

T

S

G

G

C

A

A

R

I

I

L

L

G

N

T

T

L

L

D

F

E

E

L

M

E

K

V

R

R

R

Q

G

Q

A

R

R

Y

K

G

G

L

L

A

A

T

T

L

L

C
|
C

V

I

G
|
G

G

G

M

M

G

G

I

V

A

A

T

M

I

C

I

I

E

E

R

S

L

L

active site: C88 (= C91), H347 (= H357), C377 (= C387), G379 (= G389)
binding pantothenyl-aminoethanol-11-pivalic acid: L147 (≠ F145), H155 (vs. gap), F234 (= F224), A242 (≠ P252), S246 (= S256), G247 (= G257), L248 (≠ I258), A317 (= A327), F318 (= F328), H347 (= H357)

2wkuA Biosynthetic thiolase from z. Ramigera. The n316h mutant. (see paper)
39% identity, 99% coverage: 5:401/401 of query aligns to 3:389/389 of 2wkuA

query
sites
2wkuA

L

V

I

I

F
x
A

D
x
S

A
|
A

I

A

R

R

T

T

P

-

R

-

G

A

K

V

G

G

K

S

A

F

D

N

G

G

A

A

L

F

H

A

S

N

V

T

K

P

P

A

V

H

N

E

L

L