SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF4157 FitnessBrowser__WCS417:GFF4157 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 11 hits to proteins with known functional sites (download)

P13804 Electron transfer flavoprotein subunit alpha, mitochondrial; Alpha-ETF from Homo sapiens (Human) (see 6 papers)
62% identity, 100% coverage: 2:309/309 of query aligns to 21:330/333 of P13804

query
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P13804

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G116 (= G95) to R: in GA2A; impaired protein stability and loss of electron transfer activity; dbSNP:rs119458971
T171 (= T150) to I: decreased protein stability; dbSNP:rs1801591
R223 (= R202) binding
S248 (= S227) binding
R249 (= R228) mutation to A: Loss of electron transfer activity.
VGQT 263:266 (= VGQT 242:245) binding
T266 (= T245) to M: in GA2A; decreased electron transfer activity; dbSNP:rs119458970
SGAIQH 281:286 (= SGAIQH 260:265) binding
N300 (= N279) binding
DL 318:319 (= DL 297:298) binding

Sites not aligning to the query:

20:204 Domain I
205:333 Domain II

2a1uA Crystal structure of the human etf e165betaa mutant (see paper)
62% identity, 100% coverage: 2:309/309 of query aligns to 3:312/315 of 2a1uA

query
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2a1uA

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binding flavin-adenine dinucleotide: G204 (= G201), R205 (= R202), G206 (= G203), S230 (= S227), R231 (= R228), A232 (= A229), Q244 (= Q241), V245 (= V242), G246 (= G243), Q247 (= Q244), T248 (= T245), G261 (= G258), I262 (= I259), S263 (= S260), A265 (= A262), Q267 (= Q264), H268 (= H265), N282 (= N279), K283 (= K280), D284 (= D281), A299 (= A296), D300 (= D297), L301 (= L298), F302 (= F299)

1efpA Electron transfer flavoprotein (etf) from paracoccus denitrificans (see paper)
65% identity, 89% coverage: 30:305/309 of query aligns to 29:303/307 of 1efpA

query
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1efpA

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binding flavin-adenine dinucleotide: G199 (= G201), R200 (= R202), G201 (= G203), S225 (= S227), R226 (= R228), A227 (= A229), Q239 (= Q241), V240 (= V242), G241 (= G243), Q242 (= Q244), T243 (= T245), G256 (= G258), I257 (= I259), S258 (= S260), A260 (= A262), Q262 (= Q264), H263 (= H265), N277 (= N279), K278 (= K280), D279 (= D281), G294 (≠ A296), D295 (= D297), L296 (= L298), F297 (= F299)

6fahE Molecular basis of the flavin-based electron-bifurcating caffeyl-coa reductase reaction (see paper)
34% identity, 99% coverage: 3:308/309 of query aligns to 68:391/393 of 6fahE

query
sites
6fahE

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A

Q

E

K

G

G

G

E

S

V

A

I

T

K

V

L

E

D

A

A

V

T

A

F

A

N

A

E

H

G

D

D

A

I

G

R

T

T

S

K

S

V

F

L

V

-

G

-

E

-

E

E

L

I

A

V

K

K

S

T

-

T

D

D

R

N

P

I

E

S

L

I

T

S

A

D

A

A

K

D

I

F

V

I

V

V

S

S

G

G

G
|
G

R
x
M

G
|
G

M

L

Q

G

N

K

G

P

D

E

N

G

F

F

K

E

H

L

L

L

Y

K

A

Q

L

L

A

A

D

D

K

K

L

L

G

G

A

G

A

T

V

V

G

A

A

T

S
|
S

R
|
R

A
|
A

A

C

V

V

D

D

A

A

G

G

F

W

V

A

P

D

N

H

D

A

M

Q

Q
|
Q

V
|
V

G
|
G

Q
|
Q

T
|
T

G
|
G

K

T

I

T

V

V

A

K

P

P

Q

Q

L

I

Y

Y

I

F

A

A

V

C

G
|
G

I
|
I

S
|
S

G

G

A
|
A

I

I

Q
|
Q

H
|
H

L

I

A

A

G

G

M

M

K

Q

D

D

S

S

K

D

V

I

I

I

V

I

A

A

I

I

N
|
N

K
|
K

D
x
N

E

E

E

N

A

A

P

P

I

I

F

F

Q

E

V

V

A

A

D

D

Y

Y

G

G

L

I

V

V

A
x
G

D
|
D

L
|
L

F

Y

E

K

A

V

V

I

P

P

-

A

-

I

-

E

E

E

L

L

E

D

K

K

L

I

binding flavin-adenine dinucleotide: L180 (≠ Q110), R200 (= R125), F203 (≠ Y128), I211 (≠ V136), G280 (= G201), M281 (≠ R202), G282 (= G203), S306 (= S227), R307 (= R228), A308 (= A229), Q320 (= Q241), V321 (= V242), G322 (= G243), Q323 (= Q244), T324 (= T245), G325 (= G246), G337 (= G258), I338 (= I259), S339 (= S260), A341 (= A262), Q343 (= Q264), H344 (= H265), N358 (= N279), K359 (= K280), N360 (≠ D281), G375 (≠ A296), D376 (= D297), L377 (= L298)

Sites not aligning to the query:

binding iron/sulfur cluster: 7, 8, 10, 13, 17, 18, 35, 36, 37, 38, 41, 45, 49

5ow0A Crystal structure of an electron transfer flavoprotein from geobacter metallireducens (see paper)
36% identity, 99% coverage: 1:305/309 of query aligns to 1:287/292 of 5ow0A

query
sites
5ow0A

M

M

T

K

I

T

L

L

V

I

I

I

A

E

E

T

H

A

D

N

N

A

K

K

V

I

L

L

A

G

P

-

A

-

T

E

L

L

N

V

T

T

V

V

A

S

A

R

A

L

A

F

K

G

I

Q

G

A

G

P

D

D

I

V

H

V

V

V

L

L

V

G

A

S

G

G

Q

E

G

L

A

Q

G

G

A

S

V

Y

A

G

E

K

A

A

A

Y

A

R

K

-

I

-

A

-

G

-

V

L

S

S

K

D

V

T

L

L

N

G

A

A

D

N

N

-

A

-

Y

-

A

-

H

-

Q

-

L

L

P

G

E

S

N

S

V

L

A

S

P

D

L

L

V

I

A

K

E

R

L

-

G

-

K

E

S

R

Y

Y

S

E

H

L

I

I

L

L

A

L

A

S

A

T

T

T

S

A

N

I

G

G

K

S

N

G

I

L

L

A

P

G

R

P

V

L

A

A

A

V

Q

S

L

L

D

G

V

A

D

P

Q

I

I

L

S

S

E

E

I

V

I

T

S

A

V

I

E

S

S

P

A

D

D

L

T

T

F

I

K

E

R

R

P

S

I

L

Y

Y

A

G

G

S

N

K

A

A

I

V

A

A

T

R

V

Y

Q

K

S

L

N

E

A

S

S

G

V

P

K

L

V

V

I

L

T

T

V

I

R

K

A

R

T

K

G

Y

F

F

D

E

P

A

V

A

A

T

A

L

E

E

G

G

G

T

S

T

A

A

T

T

V

E

E

E

A

L

V

P

A

V

A

G

A

P

H

Q

D

K

A

-

G

-

T

I

S

T

S

L

F

L

V

E

G

E

E

I

E

E

L

E

A

E

K

R

S

T

D

G

R

I

P

P

E

-

L

L

T

E

A

D

A

A

K

E

I

V

V

V

S

T

G

G

G
|
G

R
|
R

G
|
G

M

I

Q

G

N

S

G

G

D

D

N

N

F

F

K

S

H

I

L

L

Y

K

A

E

L

I

A

A

D

G

K

M

L

L

G

N

A

G

A

A

V

V

G

G

A

A

S
|
S

R
|
R

A
x
G

A

A

V

V

D

D

A

E

G

G

F

W

V

M

P

P

N

P

D

G

M

A

Q
|
Q

V
x
I

G
|
G

Q
|
Q

T
|
T

G

G

K

K

I

I

V

V

A

A

P

P

Q

T

L

V

Y

Y

I

F

A

A

V

V

G
|
G

I
x
V

S
|
S

G

G

A
|
A

I

S

Q
|
Q

H
|
H

L

L

A

A

G

G

M

I

K

S

D

N

S

A

K

K

V

C

I

V

V

I

A

A

I
|
I

N
|
N

K
|
K

D
|
D

E

N

E

E

A

A

P

N

I

I

F

F

Q

K

V

R

A

A

D

R

Y

F

G

G

L

I

V

V

A
x
G

D
|
D

L
x
Y

F

K

E

K

A

A

V

V

P

P

E

A

L

L

binding flavin-adenine dinucleotide: G183 (= G201), R184 (= R202), G185 (= G203), S209 (= S227), R210 (= R228), G211 (≠ A229), Q223 (= Q241), I224 (≠ V242), G225 (= G243), Q226 (= Q244), T227 (= T245), G240 (= G258), V241 (≠ I259), S242 (= S260), A244 (= A262), Q246 (= Q264), H247 (= H265), I260 (= I278), N261 (= N279), K262 (= K280), D263 (= D281), G278 (≠ A296), D279 (= D297), Y280 (≠ L298)

4kpuA Electron transferring flavoprotein of acidaminococcus fermentans: towards a mechanism of flavin-based electron bifurcation (see paper)
35% identity, 82% coverage: 53:305/309 of query aligns to 66:328/338 of 4kpuA

query
sites
4kpuA

G

G

V

A

S

D

K

L

V

V

L

Y

N

V

A

C

D

Q

N

D

A

P

A

A

Y

F

A

K

H

Y

Q

Y

L

S

P

T

E

D

N

E

V

Y

A

T

P

N

L

A

V

F

A

C

E

E

L

M

G

I

K

D

S

E

Y

Y

-

Q

-

P

S

S

H

S

I

V

L

F

A

I

A

G

A

A

T

T

S

N

N

D

G

G

K

R

N

D

I

L

L

G

P

P

R

R

V

I

A

A

Q

R

L

V

D

N

V

T

D

G

Q
x
L

I
x
C

S

A

E

D

I

C

I

T

S

I

V

L

E

D

S

A

A

E

D

E

-

D

-

G

-

L

-

I

T

E

F

W

K

T

R
|
R

P

P

I
x
A

Y

A

A

G

G

G

N

N

A

I

I

M

A
|
A

T

T

V
x
I

Q

L

S

C

N

K

A

E

-

H

S

R

V

P

K

Q

V

M

I

G

T

T

V

V

R

R

A

P

T

K

G

T

F

F

-

K

-

A

-

M

D

E

P

P

V

D

A

A

A

S

E

R

G

T

G

G

S

E

A

V

T

I

V

N

E

Y

A

T

V

L

A

K

A

N

A

H

H

V

D

D

A

D

G

R

T

V

S

T

S

C

F

I

V

R

G

R

E

E

L

V

A

V

K

S

S

E

D

G

R

E

P

M

E

A

L

I

T

D

A

D

A

A

K

P

I

F

V

V

V

C

S

S

G

G

G
|
G

R
|
R

G
|
G

M

M

Q

K

N

A

G

K

D

E

N

N

F

F

K

S

H

L

L

L

Y

Y

A

D

L

L

A

A

D

H

K

A

L

L

G

G

A

G

A

A

V

V

G

G

A

G

S
|
S

R
|
R

A
|
A

A

A

V

V

D

D

A

E

G

G

F

F

V

I

P

E

N

H

D

P

M

R

Q
|
Q

V
|
V

G
|
G

Q
|
Q

T
x
S

G

G

K

K

I

T

V

V

A

T

P

P

Q

K

L

I

Y

Y

I

F

A

A

V

C

G
|
G

I
|
I

S
|
S

G

G

A
x
S

I

V

Q
|
Q

H
|
H

L

K

A

A

G

G

M

M

K

S

D

K

S

S

K

D

V

T

I

I

V

V

A

C

I
|
I

N
|
N

K
|
K

D
|
D

E

P

E

D

A

A

P

P

I

M

F

F

Q

E

V

I

A

S

D

K

Y

Y

G

G

L

I

V

V

A
x
G

D
|
D

L
x
A

F

L

E

K

A

I

V

L

P

P

E

L

L

L

binding flavin-adenine dinucleotide: L125 (≠ Q110), C126 (≠ I111), R144 (= R125), A146 (≠ I127), A153 (= A134), I155 (≠ V136), G224 (= G201), R225 (= R202), G226 (= G203), S250 (= S227), R251 (= R228), A252 (= A229), Q264 (= Q241), V265 (= V242), G266 (= G243), Q267 (= Q244), S268 (≠ T245), G281 (= G258), I282 (= I259), S283 (= S260), S285 (≠ A262), Q287 (= Q264), H288 (= H265), I301 (= I278), N302 (= N279), K303 (= K280), D304 (= D281), G319 (≠ A296), D320 (= D297), A321 (≠ L298)

5ol2A The electron transferring flavoprotein/butyryl-coa dehydrogenase complex from clostridium difficile (see paper)
36% identity, 98% coverage: 3:305/309 of query aligns to 4:320/331 of 5ol2A

query
sites
5ol2A

I

V

L

L

V

V

I

V

A

I

E

E

H

Q

D

R

N

E

K

N

V

V

L

I

A

Q

P

T

A

V

T

S

L

L

N

E

T

L

V

L

A

G

A

K

A

A

A

T

K

E

I

I

G

A

G

K

D

D

I

Y

H

D

V

T

L

K

V

V

A

S

G

A

Q

L

G

L

A

L

G

G

A

S

V

K

A

V

E

E

A

G

A

L

A

I

K

D

I

T

A

L

-

A

-

H

-

Y

G

G

V

A

S

D

K

E

V

V

L

I

N

V

A

V

D

D

N

D

A

E

A

A

Y

L

A

A

H

V

Q

Y

L

T

P

T

E
|
E

N

P

V

Y

A

T

P

K

L

A

V

A

A

Y

E

E

L

A

G

I

K

K

S

A

Y

A

S

D

H

P

I

I

-

V

L

L

A

F

A

G

A

A

T

T

S

S

N

I

G

G

K

R

N

D

I

L

L

A

P

P

R

R

V

V

A

S

A

A

Q

R

L

I

D

H

V

T

D

G

Q
x
L

I
x
T

S

A

E

D

I

C

I

T

S

G

V

L

E

A

S

V

A

A

D

E

T

D

F

T

K

K

-

L

-

M

-

T

R
|
R

P

P

I

A

Y

F

A

G

G

G

N

N

A

I

I

M

A

A

T

T

-
x
I

V

V

Q

C

S

K

N

D

A

F

S

R

V

P

K

Q

V

M

I

S

T

T

V

V

R

R

A

P

T

-

G

-

F

-

D

G

P

V

V

M

A

K

E

N

G

E

G

P

S

D

A

E

T

T

V

K

E

E

A

A

V

V

-

I

-

N

-

R

-

F

-

K

A

V

A

E

A

F

H

N

D

D

A

A

G
x
D

T

K

S

L

S

V

F

Q

V

V

G

V

E

Q

E

V

L

I

A

K

K

E

S

A

D

K

R

K

P

Q

-

V

E

K

L

I

T

E

A

D

A

A

K

K

I

I

V

L

V

V

S

S

G

A

G
|
G

R
|
R

G
|
G

M

M

Q

G

N

G

G

K

D

E

N

N

F

L

K

D

H

I

L

L

Y

Y

A

E

L

L

A

A

D

E

K

I

L

I

G

G

A

G

A

E

V

V

G

S

A

G

S
|
S

R
|
R

A
|
A

A

T

V

I

D

D

A

A

G

G

F

W

V

L

P

D

N

K

D

A

M

R

Q
|
Q

V
|
V

G
|
G

Q
|
Q

T
|
T

G

G

K

K

I

T

V

V

A

R

P

P

Q

D

L

L

Y

Y

I

I

A

A

V

C

G
|
G

I
|
I

S
|
S

G

G

A
|
A

I

I

Q
|
Q

H
|
H

L

I

A

A

G

G

M

M

K

E

D

D

S

A

K

E

V

F

I

I

V

V

A

A

I

I

N
|
N

K
|
K

D
x
N

E

P

E

E

A

A

P

P

I

I

F

F

Q

K

V

Y

A

A

D

D

Y

V

G

G

L

I

V

V

A
x
G

D
|
D

L
x
V

F
x
H

E

K

A

V

V

L

P

P

E

E

L

L

binding calcium ion: E75 (= E71), D188 (≠ G174)
binding flavin-adenine dinucleotide: L116 (≠ Q110), T117 (≠ I111), R136 (= R125), I147 (vs. gap), G216 (= G201), R217 (= R202), G218 (= G203), S242 (= S227), R243 (= R228), A244 (= A229), Q256 (= Q241), V257 (= V242), G258 (= G243), Q259 (= Q244), T260 (= T245), G273 (= G258), I274 (= I259), S275 (= S260), A277 (= A262), Q279 (= Q264), H280 (= H265), N294 (= N279), K295 (= K280), N296 (≠ D281), G311 (≠ A296), D312 (= D297), V313 (≠ L298), H314 (≠ F299)

7qh2A Cryo-em structure of ldh-etfab complex from acetobacterium woodii (see paper)
32% identity, 99% coverage: 3:309/309 of query aligns to 13:331/337 of 7qh2A

query
sites
7qh2A

I

I

L

T

V

V

I

Y

A

V

E

D

H

H

D

I

N

E

K

G

V

Q

L

I

A

H

P

P

A

V

T

T

L

F

N

E

T

L

V

I

A

G

A

K

A

A

-

R

-

E

-

L

-

A

A

A

K

V

I

I

G

G

G

H

D

P

I

V

H

Y

V

A

L

L

V

L

A

M

G

G

Q

T

G

N

A

I

G

T

A

E

V

K

A

A

E

D

A

E

A

L

K

K

I

-

A

Y

G

G

V

V

S

D

K

K

V

V

L

F

N

V

A

Y

D

D

N

K

A

P

A

E

Y

L

A

K

H

H

Q

F

L

V

P

I

E

E

N

P

V

Y

A

A

P

N

L

V

V

L

A

E

E

D

L

F

G

I

K

E

S

K

Y

V

-

K

-

P

S

S

H

S

I

I

L

L

A

V

A

G

A

A

T

T

S

N

N

V

G

G

K

R

N

S

I

L

L

A

P

P

R

R

V

V

A

A

Q

R

L

Y

D

R

V

T

D

G

Q
x
L

I
x
T

S

A

E

D

I

C

I

T

S

I

V

L

E

E

S

M

A

K

D

E

T

N

F

T

K

D

-

L

-

V

-

Q

-

I

R
|
R

P

P

I

A

Y

F

A

G

G

G

N

N

A

I

I

M

A
|
A

T

Q

-
x
I

V

V

Q

T

S

E

N

N

A

T

S

R

V

P

K

Q

V

F

I

C

T

T

V

V

R

R

A

Y

T

K

G

V

F

F

D

T

-

A

P

P

V

E

A

R

A

V

E

N

G

E

G

P

S

W

A

G

T

D

V

V

E

E

A

M

V

M

A

D

A

I

A

E

H

K

D

A

A

K

G

L

T

V

S

S

S

A

F

I

-

E

V

V

G

M

E

E

E

V

L

I

A

K

K

K

S

E

D

K

R

G

P

I

E

D

L

L

T

S

A

E

A

A

K

E

I

T

V

I

V

V

S

A

G

V

G
|
G

R
|
R

G
|
G

M

V

Q

K

N

C

G

E

D

K

N

D

F

L

K

D

H

M

L

I

Y

H

A

E

L

F

A

A

D

E

K

K

L

I

G

G

A

A

A

T

V

V

G

A

A

C

S
x
T

R
|
R

A
x
P

A

G

V

I

D

E

A

A

G

G

F

W

V

F

P

D

N

A

D

R

M

L

Q
|
Q

V
x
I

G
|
G

Q
x
L

T
x
S

G

G

K

R

I

T

V

V

A

K

P

P

Q

K

L

L

Y

I

I

I

A

A

V

L

G
|
G

I
|
I

S
|
S

G

G

A
|
A

I

V

Q
|
Q

H
x
F

L

A

A

A

G

G

M

M

K

Q

D

N

S

S

K

E

V

Y

I

I

V

I

A

A

I
|
I

N
|
N

K
x
S

D
|
D

E

P

E

K

A

A

P

P

I

I

F

F

Q

N

V

I

A

A

D

H

Y

C

G

G

L

M

V

V

A
x
G

D
|
D

L
|
L

F
x
Y

E

E

A

I

V

L

P

P

E

E

L

L

E

L

K

T

L

M

V

I

binding flavin-adenine dinucleotide: L125 (≠ Q110), T126 (≠ I111), R144 (= R125), A153 (= A134), I155 (vs. gap), G223 (= G201), R224 (= R202), G225 (= G203), T249 (≠ S227), R250 (= R228), P251 (≠ A229), Q263 (= Q241), I264 (≠ V242), G265 (= G243), L266 (≠ Q244), S267 (≠ T245), G280 (= G258), I281 (= I259), S282 (= S260), A284 (= A262), Q286 (= Q264), F287 (≠ H265), I300 (= I278), N301 (= N279), S302 (≠ K280), D303 (= D281), G318 (≠ A296), D319 (= D297), L320 (= L298), Y321 (≠ F299)

7koeB Electron bifurcating flavoprotein fix/etfabcx (see paper)
34% identity, 98% coverage: 3:305/309 of query aligns to 7:323/336 of 7koeB

query
sites
7koeB

I

I

L

F

V

V

I

L

A

I

E

E

H

H

D

H

N

G

K

G

V

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L

A

A

H

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P

A

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W

N

E

T

L

V

I

A

G

A

K

A

A

A

R

K

D

I

L

-

A

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S

-

K

-

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-

E

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G

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I

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H

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V

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V

L

A

L

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G

Q

E

G

G

A

L

G

E

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A

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K

A

E

A

A

A

I

K

Q

I

-

A

R

G

G

V

A

S

D

K

K

V

V

L

L

N

Y

A

V

D

K

N

N

A

R

A

E

Y

F

A

N

H

T

Q

Y

L

V

P

N

E

Y

N

L

V

Y

A

K

P

K

L

A

V

L

A

V

E

D

L

M

G

V

K

R

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K

Y

Y

S

R

H

P

-

E

-

I

I

F

L

L

A

I

A

G

A

A

T

T

S

L

N

E

G

G

K

R

N

E

I

L

L

A

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M

V

V

A

A

A

T

Q

E

L

L

D

E

V

T

D

G

Q
x
L

I
x
T

S

A

E

D

-

C

-

T

-

G

-

L

-

D

I

I

S

P

V

D

E

K

S

K

A

L

D

L

T

A

F

M

K

T

R
|
R

P

P

I
x
T

Y

F

A

G

G

G

N

N

A

L

I

M

A
|
A

T

T

V
x
I

Q

M

S

C

-

P

N

D

A

H

S

R

V

P

K

Q

V

M

I

A

T

T

V

V

R

R

A

P

T

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G

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F

M

D

K

P

E

V

L

A

P

E

D

G

P

G

-

S

E

A

R

T

T

V

G

E

E

A

I

V

I

A

E

H

Y

D

D

A

L

G

G

T

T

S

F

S

D

F

K

-

L

-

I

-

E

V

I

G

L

E

E

E

T

L

I

A

P

K

L

S

Q

D

T

R

Q

P

V

E

N

L

L

T

E

A

Y

A

A

K

P

I

V

V

V

S

A

G

G

G
|
G

R
x
K

G
|
G

M

V

Q

G

N

G

G

P

D

E

N

G

F

F

K

K

H

K

L

L

Y

K

A

E

L

L

A

A

D

D

K

L

L

L

G

G

A

G

A

E

V

V

G

G

A

A

S
|
S

R
|
R

A
|
A

A

A

V

V

D

K

A

A

G

G

F

W

V

I

P

S

N

P

D

E

M

H

Q
|
Q

V
|
V

G
|
G

Q
|
Q

T
|
T

G
|
G

K

K

I

T

V

V

A

R

P

P

Q

V

L

L

Y

Y

I

F

A

A

V

C

G
|
G

I
|
I

S
|
S

G

G

A
|
A

I

I

Q
|
Q

H
|
H

L

V

A

V

G

G

M

I

K

K

D

E

S

S

K

E

V

I

I

I

V

V

A

A

I
|
I

N
|
N

K
x
I

D
|
D

E

E

E

K

A

A

P

P

I

I

F

F

Q

D

V

I

A

A

D

D

Y

I

G

G

L

I

V

V

A
x
G

D
|
D

L
|
L

F

H

E

K

A

V

V

V

P

P

E

A

L

L

binding flavin-adenine dinucleotide: L120 (≠ Q110), T121 (≠ I111), R140 (= R125), T142 (≠ I127), A149 (= A134), I151 (≠ V136), G219 (= G201), K220 (≠ R202), G221 (= G203), S245 (= S227), R246 (= R228), A247 (= A229), Q259 (= Q241), V260 (= V242), G261 (= G243), Q262 (= Q244), T263 (= T245), G264 (= G246), G276 (= G258), I277 (= I259), S278 (= S260), A280 (= A262), Q282 (= Q264), H283 (= H265), I296 (= I278), N297 (= N279), I298 (≠ K280), D299 (= D281), G314 (≠ A296), D315 (= D297), L316 (= L298)

P53571 Electron transfer flavoprotein subunit alpha; Alpha-ETF; Electron transfer flavoprotein large subunit; ETFLS from Methylophilus methylotrophus (Bacterium W3A1) (see 2 papers)
31% identity, 98% coverage: 3:306/309 of query aligns to 4:316/321 of P53571

query
sites
P53571

I

I

L

L

V

V

I

I

A

A

E

E

H

H

D

R

N

R

K

N

V

D

L

L

A

R

P

P

A

V

T

S

L

L

N

E

T

L

V

I

A

G

A

A

-

N

-

G

A

L

K

K

I

K

G

S

G

G

D

E

I

D

H

K

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V

L

V

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A

G

V

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I

G

G

A

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G

Q

A

A

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D

A

A

E

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A

V

A

P

A

A

-

L

K

S

I

V

A

N

G

G

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D

K

E

-

L

-

V

V

V

L

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A

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N

S

A

I

A

D

Y

F

A

D

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Q

D

L

V

P

F

E

E

-

A

N

S

V

V

A

S

P

A

L

L

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I

A

A

E

-

L

-

G

A

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Y

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S

H

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I

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L

A

L

A

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A

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D

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Y

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A

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D

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I

A

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E

D

I

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Y

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A

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D

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T

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F

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K

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R

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P

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-

Y

Y

A

Q

G

G

N

D

A

E

I

L

A

V

T

A

V

T

Q

R

S

G

N

G

A

Y

S

N

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Q

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K

-

V

-

N

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V

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E

-

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D

-

F

-

P

-

G

-

K

-

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T

-

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T

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I

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A

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G

V

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F

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K

P

P

V

L

A

E

A

G

E

A

G

G

G

S

S

P

-

V

A

S

T

N

V

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E

D

A

A

V

P

A

S

A

V

A

Q

H

S

D

R

A

S

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Q

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N

S

K

S

D

F

Y

V

V

G

-

E

-

E

E

L

V

A

G

K

G

S

G

D

N

R

D

P

I

E

D

L

I

T

T

A

T

A

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K

D

I

F

V

I

V

M

S

S

G

I

G

G

R
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R

G

G

M

I

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G

N

E

G

E

D

T

N

N

F

V

K

E

H

Q

L

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Y

R

A

E

L

L

A

A

D

D

K

E

L

A

G

G

A

A

A

T

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L

G

C

A

C

S
|
S

R
|
R

A

P

A

I

V

A

D

D

A

A

G

G

F

W

V

L

P

P

N

K

D

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M

R

Q
|
Q

V
|
V

G
|
G

Q
|
Q

T
x
S

G

G

K

K

I

V

V

V

-

G

A

S

P

C

Q

K

L

L

Y

Y

I

V

A

A

V

M

G
|
G

I
|
I

S
|
S

G
|
G

A
x
S

I
|
I

Q
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Q

H
|
H

L

M

A

A

G

G

M

M

K

K

D

H

S

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K

P

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T

I

I

V

I

A

A

I

V

N
|
N

K

T

D

D

E

P

E

G

A

A

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S

I

I

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F

Q

T

V

I

A

A

D

K

Y

Y

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G

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A

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D

L
x
I

F

F

E

D

A

I

V

E

P

E

E

E

L

L

E

K

R211 (= R202) binding
SR 236:237 (= SR 227:228) binding
QVGQS 250:254 (≠ QVGQT 241:245) binding
268:275 (vs. 258:265, 88% identical) binding
N289 (= N279) binding
DI 307:308 (≠ DL 297:298) binding

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

3clrD Crystal structure of the r236a etf mutant from m. Methylotrophus (see paper)
30% identity, 98% coverage: 3:306/309 of query aligns to 3:315/319 of 3clrD

query
sites
3clrD

I

I

L

L

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V

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I

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A

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E

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N

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P

A

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L

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N

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T

L

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I

A

G

A

A

-

N

-

G

A

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K

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G

G

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E

I

D

H

K

V

V

L

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A

A

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G

G

A

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G

Q

A

A

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D

A

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A

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A

P

A

A

-

L

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V

A

N

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G

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E

-

L

-

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V

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A

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N

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A

I

A

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Y

F

A

D

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P

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D

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F

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E

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A

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A

L

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V

I

A

A

E

-

L

-

G

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Y

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V

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L

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L

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D

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A

A

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D

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E

S

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A

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-

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-

Y

Y

A

Q

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E

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L

A

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A

V

T

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R

S

G

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G

A

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K

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-

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-

V

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E

-

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A

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V

F

F

D

K

P

P

V

L

A

E

A

G

E

A

G

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G

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P

-

V

A

S

T

N

V

V

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D

A

A

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A

S

A

V

A

Q

H

S

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R

A

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T

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D

F

Y

V

V

G

-

E

-

E

E

L

V

A

G

K

G

S

G

D

N

R

D

P

I

E

D

L

I

T

T

A

T

A

V

K

D

I

F

V

I

V

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S

S

G

I

G
|
G

R
|
R

G
|
G

M

I

Q

G

N

E

G

E

D

T

N

N

F

V

K

E

H

Q

L

F

Y

R

A

E

L

L

A

A

D

D

K

E

L

A

G

G

A

A

A

T

V

L

G

C

A

C

S
|
S

R
x
A

A
x
P

A

I

V

A

D

D

A

A

G

G

F

W

V

L

P

P

N

K

D

S

M

R

Q
|
Q

V
|
V

G
|
G

Q
|
Q

T
x
S

G
|
G

K

K

I

V

V

V

-

G

A

S

P

C

Q

K

L

L

Y

Y

I

V

A

A

V

M

G
|
G

I
|
I

S
|
S

G

G

A
x
S

I

I

Q
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Q

H
|
H

L

M

A

A

G

G

M

M

K

K

D

H

S

V

K

P

V

T

I

I

V

I

A

A

I

V

N
|
N

K
x
T

D
|
D

E

P

E

G

A

A

P

S

I

I

F

F

Q

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V

I

A

A

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Y

Y

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A
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A

D
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D

L
x
I

F
|
F

E

D

A

I

V

E

P

E

E

E

L

L

E

K

binding flavin-adenine dinucleotide: G209 (= G201), R210 (= R202), G211 (= G203), S235 (= S227), A236 (≠ R228), P237 (≠ A229), Q249 (= Q241), V250 (= V242), G251 (= G243), Q252 (= Q244), S253 (≠ T245), G254 (= G246), G267 (= G258), I268 (= I259), S269 (= S260), S271 (≠ A262), Q273 (= Q264), H274 (= H265), N288 (= N279), T289 (≠ K280), D290 (= D281), A305 (= A296), D306 (= D297), I307 (≠ L298), F308 (= F299)

Query Sequence

>GFF4157 FitnessBrowser__WCS417:GFF4157
MTILVIAEHDNKVLAPATLNTVAAAAKIGGDIHVLVAGQGAGAVAEAAAKIAGVSKVLNA
DNAAYAHQLPENVAPLVAELGKSYSHILAAATSNGKNILPRVAAQLDVDQISEIISVESA
DTFKRPIYAGNAIATVQSNASVKVITVRATGFDPVAAEGGSATVEAVAAAHDAGTSSFVG
EELAKSDRPELTAAKIVVSGGRGMQNGDNFKHLYALADKLGAAVGASRAAVDAGFVPNDM
QVGQTGKIVAPQLYIAVGISGAIQHLAGMKDSKVIVAINKDEEAPIFQVADYGLVADLFE
AVPELEKLV

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory