SitesBLAST
Comparing GFF4161 FitnessBrowser__psRCH2:GFF4161 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
I6YCA3 Acyl-CoA dehydrogenase FadE26; ACAD; 3-oxocholest-4-en-26-oyl-CoA dehydrogenase alpha subunit; EC 1.3.99.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
34% identity, 100% coverage: 1:398/398 of query aligns to 1:395/400 of I6YCA3
- IGYS 127:130 (≠ QGYS 126:129) binding
- T136 (≠ S135) binding
- S162 (≠ T161) binding
- E247 (= E244) mutation to A: Loss of dehydrogenase activity.
- TNE 380:382 (≠ SNE 383:385) binding
4x28A Crystal structure of the chse4-chse5 complex from mycobacterium tuberculosis (see paper)
34% identity, 100% coverage: 1:398/398 of query aligns to 1:382/386 of 4x28A
- active site: Y122 (= Y128), S123 (= S129), E240 (= E244), G365 (= G381), M377 (≠ K393)
- binding dihydroflavine-adenine dinucleotide: I120 (≠ Q126), Y122 (= Y128), S123 (= S129), G128 (= G134), T129 (≠ S135), W153 (= W159), S155 (≠ T161), F363 (≠ I379), T367 (≠ S383), E369 (= E385), V370 (≠ I386)
P71858 Acyl-CoA dehydrogenase FadE29; ACAD; 3-oxo-23,24-bisnorchol-4-en-22-oyl-CoA dehydrogenase beta subunit; 3-oxo-4-pregnene-20-carboxyl-CoA dehydrogenase beta subunit; EC 1.3.99.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
32% identity, 100% coverage: 1:398/398 of query aligns to 1:382/387 of P71858
- E241 (= E244) mutation to Q: Unable to dehydrogenate pregnene-carboxyl-CoA ester.
6wy8B Tcur3481-tcur3483 steroid acad (see paper)
33% identity, 100% coverage: 1:398/398 of query aligns to 1:381/384 of 6wy8B
- active site: Y126 (= Y128), T127 (≠ S129), E241 (= E244), T376 (≠ K393)
- binding flavin-adenine dinucleotide: I124 (≠ Q126), Y126 (= Y128), T127 (≠ S129), G132 (= G134), T133 (≠ S135), F157 (≠ W159), S159 (≠ T161), V359 (≠ F380), F362 (vs. gap), G363 (vs. gap), V366 (≠ S383), E368 (= E385)
P96855 Acyl-CoA dehydrogenase FadE34; ACAD; 3-oxochol-4-en-24-oyl-CoA dehydrogenase; EC 1.3.99.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
35% identity, 89% coverage: 43:398/398 of query aligns to 381:709/711 of P96855
- E581 (= E244) mutation to Q: Displays less than 1% activity with cholyl-CoA as substrate. Still binds FAD.
Sites not aligning to the query:
- 236 R→A: Displays less than 2% activity with cholyl-CoA as substrate. Cannot bind FAD.
6wy9A Tcur3481-tcur3483 steroid acad g363a variant (see paper)
33% identity, 97% coverage: 12:398/398 of query aligns to 8:377/380 of 6wy9A
- active site: Y122 (= Y128), T123 (≠ S129), E237 (= E244), T372 (≠ K393)
- binding dihydroflavine-adenine dinucleotide: I120 (≠ Q126), Y122 (= Y128), T123 (≠ S129), G128 (= G134), T129 (≠ S135), F153 (≠ W159), S155 (≠ T161), F358 (vs. gap), V362 (≠ S383), E364 (= E385)
2pg0A Crystal structure of acyl-coa dehydrogenase from geobacillus kaustophilus
28% identity, 86% coverage: 52:395/398 of query aligns to 47:378/380 of 2pg0A
- active site: M124 (≠ Y128), T125 (≠ S129), E243 (= E244), A364 (≠ G381), R376 (≠ K393)
- binding flavin-adenine dinucleotide: I122 (≠ Q126), M124 (≠ Y128), T125 (≠ S129), G130 (= G134), S131 (= S135), F155 (≠ W159), I156 (≠ T160), T157 (= T161), R269 (≠ E268), F272 (≠ D271), F279 (≠ D278), Q337 (≠ K336), L338 (≠ V337), G340 (= G339), G341 (≠ P340), V359 (≠ K376), I362 (= I379), Y363 (≠ F380), T366 (≠ S383), E368 (= E385), M369 (≠ I386)
8hk0B Crystal structure of fic32-33 complex from streptomyces ficellus nrrl 8067 (see paper)
33% identity, 62% coverage: 1:248/398 of query aligns to 1:247/379 of 8hk0B
Sites not aligning to the query:
3r7kA Crystal structure of a probable acyl coa dehydrogenase from mycobacterium abscessus atcc 19977 / dsm 44196 (see paper)
29% identity, 98% coverage: 6:395/398 of query aligns to 6:377/378 of 3r7kA
- active site: V126 (≠ Y128), T127 (≠ S129), E242 (= E244), G363 (= G381), K375 (= K393)
- binding dihydroflavine-adenine dinucleotide: V126 (≠ Y128), T127 (≠ S129), G132 (= G134), S133 (= S135), F157 (≠ W159), I158 (≠ T160), T159 (= T161), R268 (≠ E268), T270 (≠ C270), F271 (≠ D271), L275 (≠ M275), R278 (≠ D278), I281 (≠ F281), Q336 (≠ L352), I337 (≠ D353), G340 (= G356), I358 (≠ K376), T365 (≠ S383), E367 (= E385)
4iv6B X-ray crystal structure of an isovaleryl-coa dehydrogenase from mycobacterium smegmatis (see paper)
30% identity, 76% coverage: 93:396/398 of query aligns to 86:376/383 of 4iv6B
- active site: L121 (≠ Y128), T122 (≠ S129), G240 (≠ E244), E361 (vs. gap), K373 (= K393)
- binding dihydroflavine-adenine dinucleotide: L121 (≠ Y128), T122 (≠ S129), G126 (≠ A133), G127 (= G134), S128 (= S135), W152 (= W159), I153 (≠ T160), S154 (≠ T161), R266 (≠ E268), S268 (≠ C270), F269 (≠ D271), I273 (≠ M275), H276 (≠ D278), V279 (≠ F281), R334 (≠ L358), V335 (≠ L359), G338 (≠ D362), L356 (= L377), G360 (= G381), T363 (≠ S383), E365 (= E385), I366 (= I386)
8sgrA Isovaleryl-CoA dehydrogenase, mitochondrial (see paper)
29% identity, 76% coverage: 6:309/398 of query aligns to 13:320/393 of 8sgrA
Sites not aligning to the query:
1ivhA Structure of human isovaleryl-coa dehydrogenase at 2.6 angstroms resolution: structural basis for substrate specificity (see paper)
31% identity, 68% coverage: 40:309/398 of query aligns to 41:316/387 of 1ivhA
- active site: M130 (≠ Y128), S131 (= S129), E249 (= E244)
- binding coenzyme a persulfide: S137 (= S135), S185 (≠ Q182), R186 (= R183), V239 (≠ W234), Y240 (≠ T235), M243 (≠ K238), E249 (= E244), R250 (= R245)
- binding flavin-adenine dinucleotide: L128 (≠ Q126), M130 (≠ Y128), S131 (= S129), G136 (= G134), S137 (= S135), W161 (= W159), T163 (= T161), R275 (≠ K267), F278 (≠ C270), F285 (≠ D278), M288 (≠ F281)
Sites not aligning to the query:
P26440 Isovaleryl-CoA dehydrogenase, mitochondrial; IVD; Butyryl-CoA dehydrogenase; EC 1.3.8.4; EC 1.3.8.1 from Homo sapiens (Human) (see 5 papers)
29% identity, 76% coverage: 6:309/398 of query aligns to 46:353/426 of P26440
- 165:174 (vs. 126:135, 60% identical) binding
- S174 (= S135) binding
- WIT 198:200 (≠ WTT 159:161) binding
- SR 222:223 (≠ QR 182:183) binding
- G250 (≠ E210) to A: in IVA; uncertain significance
- Y277 (≠ T235) binding
- DLER 284:287 (≠ THER 242:245) binding
- E286 (= E244) active site, Proton acceptor; mutation to D: Residual isovaleryl-CoA dehydrogenase activity.; mutation to G: Loss of isovaleryl-CoA dehydrogenase activity. Does not affect isovaleryl-CoA dehydrogenase activity; when associated with 407-E.; mutation to Q: Loss of isovaleryl-CoA dehydrogenase activity.
- A291 (= A249) to V: in IVA; uncertain significance; dbSNP:rs886042098
- R312 (≠ E268) binding
- Q323 (≠ P279) binding
Sites not aligning to the query:
- 1:32 modified: transit peptide, Mitochondrion
- 379 I → T: in IVA; uncertain significance
- 380:384 binding
- 398 R → Q: in IVA; uncertain significance; dbSNP:rs1477527791
- 403 Y → N: in IVA; uncertain significance
- 407 A→E: Does not affect isovaleryl-CoA dehydrogenase activity; when associated with 286-D.
- 407:408 binding
- 409:411 binding
2z1qB Crystal structure of acyl coa dehydrogenase
28% identity, 86% coverage: 54:396/398 of query aligns to 69:409/549 of 2z1qB
- active site: L144 (vs. gap), T145 (≠ S129), G259 (≠ E244), E394 (≠ G381), G406 (≠ K393)
- binding flavin-adenine dinucleotide: Y142 (= Y128), L144 (vs. gap), T145 (≠ S129), G150 (= G134), S151 (= S135), W177 (= W159), S179 (≠ T161), R285 (≠ E268), F288 (≠ D271), I292 (≠ M275), F295 (≠ D278), I298 (≠ F281), H369 (= H360), G370 (≠ A361), F393 (= F380), I399 (= I386)
Sites not aligning to the query:
1ukwB Crystal structure of medium-chain acyl-coa dehydrogenase from thermus thermophilus hb8
30% identity, 84% coverage: 61:396/398 of query aligns to 56:377/379 of 1ukwB
- active site: L124 (≠ Y128), S125 (= S129), T241 (≠ E244), E362 (≠ G381), R374 (≠ K393)
- binding cobalt (ii) ion: D145 (= D149), H146 (= H150)
- binding flavin-adenine dinucleotide: F122 (≠ Q126), L124 (≠ Y128), S125 (= S129), G130 (= G134), S131 (= S135), W155 (= W159), S157 (≠ T161), K200 (≠ L205), L357 (≠ A365), Y361 (≠ F380), E362 (≠ G381), T364 (≠ S383), E366 (= E385), L370 (≠ N389)
1ukwA Crystal structure of medium-chain acyl-coa dehydrogenase from thermus thermophilus hb8
30% identity, 84% coverage: 61:396/398 of query aligns to 56:377/379 of 1ukwA
- active site: L124 (≠ Y128), S125 (= S129), T241 (≠ E244), E362 (≠ G381), R374 (≠ K393)
- binding flavin-adenine dinucleotide: F122 (≠ Q126), L124 (≠ Y128), S125 (= S129), G130 (= G134), S131 (= S135), W155 (= W159), S157 (≠ T161), L357 (≠ A365), Y361 (≠ F380), E362 (≠ G381), T364 (≠ S383), E366 (= E385), L370 (≠ N389)
3p4tA Crystal structure of a putative acyl-coa dehydrogenase from mycobacterium smegmatis (see paper)
31% identity, 75% coverage: 6:302/398 of query aligns to 5:296/381 of 3p4tA
- active site: I125 (≠ Y128), T126 (≠ S129), E241 (= E244)
- binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl (2R,3S,4S)-5-[(4aS,10aR)-7,8-dimethyl-2,4-dioxo-1,3,4,4a,5,10a-hexahydrobenzo[g]pteridin-10(2H)-yl]-2,3,4-trihydroxypentyl dihydrogen diphosphate: L123 (≠ Q126), I125 (≠ Y128), T126 (≠ S129), G130 (≠ A133), G131 (= G134), S132 (= S135), Y156 (≠ W159), I157 (≠ T160), T158 (= T161), K200 (≠ L205), R267 (≠ K273), T269 (≠ M275), L274 (= L280), R277 (≠ A283)
Sites not aligning to the query:
- active site: 363, 375
- binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl (2R,3S,4S)-5-[(4aS,10aR)-7,8-dimethyl-2,4-dioxo-1,3,4,4a,5,10a-hexahydrobenzo[g]pteridin-10(2H)-yl]-2,3,4-trihydroxypentyl dihydrogen diphosphate: 336, 337, 340, 358, 365
3oibA Crystal structure of a putative acyl-coa dehydrogenase from mycobacterium smegmatis, iodide soak (see paper)
31% identity, 75% coverage: 6:302/398 of query aligns to 5:296/381 of 3oibA
- active site: I125 (≠ Y128), T126 (≠ S129), E241 (= E244)
- binding dihydroflavine-adenine dinucleotide: L123 (≠ Q126), I125 (≠ Y128), T126 (≠ S129), G131 (= G134), S132 (= S135), Y156 (≠ W159), I157 (≠ T160), T158 (= T161), K200 (≠ L205), R267 (≠ K273), T269 (≠ M275), L274 (= L280), R277 (≠ A283)
Sites not aligning to the query:
8i4rA Crystal structure of acyl-coa dehydrogenase complexed with acetyl-coa from thermobifida fusca
27% identity, 98% coverage: 6:397/398 of query aligns to 7:381/381 of 8i4rA
- binding acetyl coenzyme *a: S132 (= S135), T134 (≠ L137), R180 (= R183), R234 (≠ T235), L237 (≠ K238), R238 (≠ Y239), L240 (= L241), D241 (≠ T242), R244 (= R245), E365 (≠ G381), G366 (= G382), R377 (≠ K393)
- binding flavin-adenine dinucleotide: Y123 (≠ Q126), L125 (≠ Y128), S126 (= S129), G131 (= G134), S132 (= S135), W156 (= W159), I157 (≠ T160), T158 (= T161), I360 (≠ K376), T367 (≠ S383), Q369 (≠ E385)
8i4pA Crystal structure of acyl-coa dehydrogenase from thermobifida fusca
27% identity, 98% coverage: 6:397/398 of query aligns to 7:381/381 of 8i4pA
- binding flavin-adenine dinucleotide: Y123 (≠ Q126), L125 (≠ Y128), S126 (= S129), G131 (= G134), S132 (= S135), W156 (= W159), I157 (≠ T160), T158 (= T161), I360 (≠ K376), Y364 (≠ F380), T367 (≠ S383), Q369 (≠ E385)
Query Sequence
>GFF4161 FitnessBrowser__psRCH2:GFF4161
MNVNYTAEELAFRDEVRAFLKSELPADIAAKVKLGKHMSKQDHERWQQILVKRGWYAPGW
PVELGGTTWGPVEKHIFDEECSAFGAPRTVPFGVNMVAPVIIKFGTQQQIDHYLPRILSG
EDWWCQGYSEPGAGSDLASLKTRAVRDGDHYVVNGQKTWTTLGQHANMIFCLVRTDPEAQ
QQRGISFLLIDMKSPGISVRPIITLDGDHEVNEVFFDNVRVPVENLVGEENQGWTCAKYL
LTHERTGLAGIGSSKAVLAHLKRIAMKEVCDGKPMLEDPLFRAQVAEVEMQLMAIEMSTL
RILAAAKEGGVPGAESSILKVKGTEIRQAITHLLRKVIGPYALPFLEEEMQLDYDGELLH
ADYSASLAGDYFNMRKLSIFGGSNEIQKNIVSKMILEL
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory