SitesBLAST
Comparing GFF42 FitnessBrowser__Marino:GFF42 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
5jy1A Crystal structure of putative short-chain dehydrogenase/reductase from burkholderia xenovorans lb400 bound to NAD
72% identity, 100% coverage: 1:266/266 of query aligns to 1:266/266 of 5jy1A
- active site: G17 (= G17), S143 (= S143), H153 (= H153), Y156 (= Y156), K160 (= K160), P201 (= P201)
- binding nicotinamide-adenine-dinucleotide: G13 (= G13), G17 (= G17), I18 (= I18), E37 (= E37), I38 (≠ L38), T62 (= T62), D63 (= D63), V64 (= V64), N90 (= N90), A91 (= A91), F92 (= F92), V93 (≠ A93), S143 (= S143), Y156 (= Y156), K160 (= K160), P186 (= P186), T187 (= T187), A188 (= A188), A191 (= A191)
4nbuB Crystal structure of fabg from bacillus sp (see paper)
38% identity, 94% coverage: 1:251/266 of query aligns to 2:244/244 of 4nbuB
- active site: G18 (= G17), N111 (≠ T115), S139 (= S143), Q149 (≠ H153), Y152 (= Y156), K156 (= K160)
- binding acetoacetyl-coenzyme a: D93 (≠ N97), K98 (≠ E102), S139 (= S143), N146 (≠ A150), V147 (≠ W151), Q149 (≠ H153), Y152 (= Y156), F184 (≠ A191), M189 (≠ L196), K200 (≠ R206)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G13), N17 (≠ G16), G18 (= G17), I19 (= I18), D38 (≠ E37), F39 (≠ L38), V59 (≠ T62), D60 (= D63), V61 (= V64), N87 (= N90), A88 (= A91), G89 (≠ A93), I90 (≠ P94), T137 (≠ F141), S139 (= S143), Y152 (= Y156), K156 (= K160), P182 (= P186), F184 (≠ A191), T185 (= T192), T187 (≠ H194), M189 (≠ L196)
6ixmC Crystal structure of the ketone reductase chkred20 from the genome of chryseobacterium sp. Ca49 complexed with NAD (see paper)
36% identity, 92% coverage: 3:248/266 of query aligns to 2:244/248 of 6ixmC
- active site: G16 (= G17), S142 (= S143), Y155 (= Y156), K159 (= K160)
- binding nicotinamide-adenine-dinucleotide: G12 (= G13), S15 (≠ G16), G16 (= G17), I17 (= I18), D36 (≠ E37), I37 (≠ L38), A61 (≠ T62), D62 (= D63), T63 (≠ V64), N89 (= N90), A90 (= A91), M140 (≠ F141), S142 (= S143), Y155 (= Y156), K159 (= K160), P185 (= P186), A186 (≠ T187), Y187 (≠ A188), I188 (≠ M189), L192 (≠ G202)
7wbcA Hydroxysteroid dehydrogenase wild-type complexed with NAD+ and (4s)-2- 2-methyl-2,4-pentanediol
38% identity, 92% coverage: 4:248/266 of query aligns to 3:245/250 of 7wbcA
- binding calcium ion: Y115 (≠ W117), P116 (≠ A118), H119 (≠ W121)
- binding nicotinamide-adenine-dinucleotide: G12 (= G13), G16 (= G17), I17 (= I18), D36 (≠ E37), V37 (≠ L38), A61 (≠ T62), D62 (= D63), I63 (≠ V64), N89 (= N90), F138 (= F141), S140 (= S143), Y153 (= Y156), K157 (= K160), P183 (= P186), F184 (≠ T187), A185 (= A188), T187 (≠ G190), G189 (≠ T192), V190 (≠ F193)
7do7A Crystal structure of azotobacter vinelandii l-rhamnose 1- dehydrogenase(NAD and l-rhamnose bound-form) (see paper)
38% identity, 94% coverage: 2:251/266 of query aligns to 1:253/256 of 7do7A
- active site: G16 (= G17), S146 (= S143), Y159 (= Y156)
- binding nicotinamide-adenine-dinucleotide: G12 (= G13), R15 (≠ G16), G16 (= G17), I17 (= I18), S37 (≠ L38), D66 (= D63), A67 (≠ V64), N93 (= N90), A94 (= A91), G95 (≠ A93), I96 (≠ P94), V144 (≠ F141), S145 (≠ Y142), S146 (= S143), Y159 (= Y156), K163 (= K160), P189 (= P186), G190 (≠ T187), I192 (≠ M189), T194 (≠ A191), I196 (≠ F193)
- binding beta-L-rhamnopyranose: F99 (≠ N97), S146 (= S143), S148 (≠ D145), Q156 (≠ H153), Y159 (= Y156), N197 (≠ H194), D235 (≠ E233), M236 (= M234), R238 (= R236)
7b81A Crystal structure of azotobacter vinelandii l-rhamnose 1-dehydrogenase (NAD bound-form) (see paper)
38% identity, 94% coverage: 2:251/266 of query aligns to 1:253/256 of 7b81A
- active site: G16 (= G17), S146 (= S143), Y159 (= Y156)
- binding nicotinamide-adenine-dinucleotide: G12 (= G13), S14 (≠ G15), R15 (≠ G16), I17 (= I18), D66 (= D63), A67 (≠ V64), N93 (= N90), A94 (= A91), G95 (≠ A93), I96 (≠ P94), T116 (= T114), V144 (≠ F141), S146 (= S143), Y159 (= Y156), K163 (= K160), P189 (= P186), G190 (≠ T187), I192 (≠ M189), T194 (≠ A191), I196 (≠ F193)
C1DMX5 L-rhamnose 1-dehydrogenase (NAD(P)(+)); RhaDH; AvLRA1; EC 1.1.1.378 from Azotobacter vinelandii (strain DJ / ATCC BAA-1303) (see paper)
38% identity, 94% coverage: 3:251/266 of query aligns to 2:253/256 of C1DMX5
- G12 (= G13) binding NADP(+)
- S14 (≠ G15) binding NADP(+)
- R15 (≠ G16) binding NADP(+); mutation to T: Increases specificity toward NAD(+). Shows a strong decrease in catalytic efficiency with NADP(+).
- I17 (= I18) binding NADP(+)
- S37 (≠ L38) binding NADP(+); mutation to H: Increases specificity toward NAD(+). Shows a strong decrease in catalytic efficiency with NADP(+) and an increase in catalytic efficiency with NAD(+).
- D66 (= D63) binding NADP(+)
- A67 (≠ V64) binding NADP(+)
- N93 (= N90) binding NADP(+)
- F99 (≠ N97) mutation F->A,Y: Shows a strong decrease in catalytic efficiency with L-rhamnose, L-lyxose and L-mannose.
- S146 (= S143) binding beta-L-rhamnose
- S148 (≠ D145) binding beta-L-rhamnose
- Q156 (≠ H153) binding beta-L-rhamnose; mutation to A: Almost loss of activity with L-rhamnose as substrate.
- Y159 (= Y156) binding beta-L-rhamnose; binding NADP(+)
- K163 (= K160) binding NADP(+)
- T191 (≠ A188) binding beta-L-rhamnose; mutation to F: Retains 4% of wild-type activity with L-rhamnose as substrate.
- I192 (≠ M189) binding NADP(+)
- I196 (≠ F193) mutation to A: Shows a strong decrease in catalytic efficiency with L-rhamnose as substrate, but does not affect catalytic efficiency with L-lyxose and L-mannose.
- N197 (≠ H194) binding beta-L-rhamnose
- D200 (≠ A197) mutation to A: Retains 16% of wild-type activity with L-rhamnose as substrate.; mutation to H: Retains 22% of wild-type activity with L-rhamnose as substrate.
7do6A Crystal structure of azotobacter vinelandii l-rhamnose 1- dehydrogenase(NADP bound-form) (see paper)
38% identity, 94% coverage: 2:251/266 of query aligns to 1:244/247 of 7do6A
- active site: G16 (= G17), S146 (= S143), Y159 (= Y156)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G13), S14 (≠ G15), R15 (≠ G16), G16 (= G17), I17 (= I18), H36 (≠ E37), S37 (≠ L38), G42 (= G43), D66 (= D63), A67 (≠ V64), N93 (= N90), A94 (= A91), G95 (≠ A93), I96 (≠ P94), T116 (= T114), S146 (= S143), Y159 (= Y156), K163 (= K160), I192 (≠ M189)
4fn4A Short-chain NAD(h)-dependent dehydrogenase/reductase from sulfolobus acidocaldarius (see paper)
36% identity, 92% coverage: 4:249/266 of query aligns to 5:251/254 of 4fn4A
- active site: G18 (= G17), S144 (= S143), Y157 (= Y156), K161 (= K160), S202 (vs. gap)
- binding nicotinamide-adenine-dinucleotide: G14 (= G13), S17 (≠ G16), G18 (= G17), I19 (= I18), E38 (= E37), L39 (= L38), R43 (≠ L42), A63 (≠ T62), D64 (= D63), V65 (= V64), N91 (= N90), G93 (≠ F92), I94 (≠ A93), T142 (≠ F141), S144 (= S143), Y157 (= Y156), K161 (= K160), P187 (= P186), V190 (≠ M189), T192 (= T192), N193 (≠ F193), I194 (≠ H194)
4urfB Molecular genetic and crystal structural analysis of 1-(4- hydroxyphenyl)-ethanol dehydrogenase from aromatoleum aromaticum ebn1 (see paper)
35% identity, 92% coverage: 3:248/266 of query aligns to 2:244/248 of 4urfB
- active site: G16 (= G17), S142 (= S143), I152 (≠ H153), Y155 (= Y156), K159 (= K160)
- binding 3-pyridinium-1-ylpropane-1-sulfonate: L210 (= L213), R211 (≠ G214), R212 (= R215)
- binding bicarbonate ion: I92 (vs. gap), G94 (vs. gap), R109 (≠ Q110), R179 (= R180), S228 (= S232)
- binding nicotinamide-adenine-dinucleotide: G12 (= G13), G14 (= G15), N15 (≠ G16), G16 (= G17), I17 (= I18), D36 (≠ E37), I37 (≠ L38), D62 (= D63), T63 (≠ V64), N89 (= N90), A90 (= A91), G91 (vs. gap), I140 (≠ F141), Y155 (= Y156), K159 (= K160), P185 (= P186), A186 (≠ T187), I188 (≠ M189), T190 (≠ A191)
4urfA Molecular genetic and crystal structural analysis of 1-(4- hydroxyphenyl)-ethanol dehydrogenase from aromatoleum aromaticum ebn1 (see paper)
35% identity, 92% coverage: 3:248/266 of query aligns to 2:244/248 of 4urfA
- active site: G16 (= G17), S142 (= S143), I152 (≠ H153), Y155 (= Y156), K159 (= K160)
- binding 3-pyridinium-1-ylpropane-1-sulfonate: I92 (vs. gap), S93 (vs. gap), G94 (vs. gap), E95 (vs. gap), T97 (≠ A93), E101 (= E102), T103 (= T104), Q106 (≠ M107), R109 (≠ Q110), S175 (≠ R176), G177 (≠ N178)
- binding magnesium ion: S237 (≠ E241), Y238 (≠ S242)
- binding nicotinamide-adenine-dinucleotide: G12 (= G13), G14 (= G15), N15 (≠ G16), G16 (= G17), I17 (= I18), D36 (≠ E37), I37 (≠ L38), W41 (≠ L42), D62 (= D63), T63 (≠ V64), N89 (= N90), A90 (= A91), G91 (vs. gap), I140 (≠ F141), Y155 (= Y156), K159 (= K160), P185 (= P186), I188 (≠ M189), T190 (≠ A191)
4ureB Molecular genetic and crystal structural analysis of 1-(4- hydroxyphenyl)-ethanol dehydrogenase from aromatoleum aromaticum ebn1 (see paper)
35% identity, 92% coverage: 3:248/266 of query aligns to 2:244/248 of 4ureB
- active site: G16 (= G17), S142 (= S143), I152 (≠ H153), Y155 (= Y156), K159 (= K160)
- binding 3-pyridinium-1-ylpropane-1-sulfonate: N15 (≠ G16), G16 (= G17), I17 (= I18), N89 (= N90), G91 (vs. gap), Y155 (= Y156), P185 (= P186), A186 (≠ T187)
G9FRD7 7alpha-hydroxysteroid dehydrogenase; 7alpha-HSDH; NADP-dependent 7alpha-hydroxysteroid dehydrogenase; EC 1.1.1.- from Clostridium sardiniense (Clostridium absonum) (see 2 papers)
38% identity, 96% coverage: 1:255/266 of query aligns to 1:257/262 of G9FRD7
- SSTRGI 13:18 (≠ GAGGGI 13:18) binding NADP(+)
- R38 (≠ L38) binding NADP(+); mutation to D: Loss of catalytic activity.
- NA 63:64 (≠ DV 63:64) binding NADP(+)
- N90 (= N90) binding NADP(+)
- T145 (≠ S143) binding taurochenodeoxycholate
- Y158 (= Y156) binding NADP(+); binding taurochenodeoxycholate
- K162 (= K160) binding NADP(+)
- IGT---RA 191:195 (≠ MGATFHKL 189:196) binding NADP(+)
Sites not aligning to the query:
- 261:262 mutation Missing: 5-fold reduction in catalytic efficiency.
Q9C826 Xanthoxin dehydrogenase; Protein ABSCISIC ACID DEFICIENT 2; Protein GLUCOSE INSENSITIVE 1; Protein IMPAIRED SUCROSE INDUCTION 4; Protein SALOBRENO 3; Protein SALT RESISTANT 1; Protein SUGAR INSENSITIVE 4; Short-chain alcohol dehydrogenase ABA2; Short-chain dehydrogenase reductase 1; AtSDR1; Xanthoxin oxidase; EC 1.1.1.288 from Arabidopsis thaliana (Mouse-ear cress) (see 3 papers)
38% identity, 92% coverage: 4:249/266 of query aligns to 18:274/285 of Q9C826
- G28 (≠ A14) mutation to R: In aba2-12/sre1-2; reduced absicic acid synthesis.
- A45 (= A31) mutation to V: In aba2-4/sis4-2; reduced absicic acid synthesis.
- R145 (≠ P128) mutation to C: In gin1-2; reduced absicic acid synthesis.
- G162 (≠ D145) mutation to R: In aba2-3/sis4-1; reduced absicic acid synthesis.
- S176 (≠ A159) mutation to F: In aba2-13/san3-1; reduced absicic acid synthesis.
- A236 (≠ R211) mutation to V: In isi4; reduced absicic acid synthesis.
- S264 (≠ T239) mutation to N: In aba2-1; reduced absicic acid synthesis.
5epoA The three-dimensional structure of clostridium absonum 7alpha- hydroxysteroid dehydrogenase (see paper)
38% identity, 95% coverage: 4:255/266 of query aligns to 3:256/261 of 5epoA
- active site: G16 (= G17), T144 (≠ S143), I152 (≠ W151), Y157 (= Y156), K161 (= K160), R193 (≠ K195)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: S12 (≠ G13), T14 (≠ G15), R15 (≠ G16), G16 (= G17), I17 (= I18), R37 (≠ L38), F61 (≠ T62), N62 (≠ D63), N89 (= N90), Y90 (≠ F92), G91 (≠ A93), Y157 (= Y156), K161 (= K160), P187 (= P186), G188 (≠ T187), I190 (≠ M189), T192 (≠ A191), R193 (≠ K195), A194 (≠ L196), A195 (= A197)
- binding taurochenodeoxycholic acid: T93 (= T95), T144 (≠ S143), G146 (≠ D145), R154 (≠ H153), Y157 (= Y156), G188 (≠ T187), N198 (= N200), M199 (≠ P201), F203 (≠ E205)
8y83A Crystal structure of a ketoreductase from sphingobacterium siyangense sy1 with co-enzyme
34% identity, 93% coverage: 1:248/266 of query aligns to 1:245/249 of 8y83A