SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF440 FitnessBrowser__Marino:GFF440 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P0A9C5 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I beta; GSI beta; EC 6.3.1.2 from Escherichia coli (strain K12) (see 3 papers)
69% identity, 99% coverage: 4:467/467 of query aligns to 6:469/469 of P0A9C5

query
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P0A9C5

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Y398 (= Y396) modified: O-AMP-tyrosine

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

P0A1P6 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I beta; GSI beta; EC 6.3.1.2 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 4 papers)
68% identity, 99% coverage: 4:467/467 of query aligns to 6:469/469 of P0A1P6

query
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P0A1P6

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E130 (= E128) binding Mn(2+)
E132 (= E130) binding Mn(2+)
E208 (= E206) binding ATP
E213 (= E211) binding Mn(2+)
E221 (= E219) binding Mn(2+)
NG 265:266 (= NG 263:264) binding L-glutamate
H270 (= H268) binding Mn(2+)
HMS 272:274 (= HMS 270:272) binding ATP
R322 (= R320) binding L-glutamate
E358 (= E356) binding Mn(2+)
R360 (= R358) binding L-glutamate

1fpyA Crystal structure of glutamine synthetase from salmonella typhimurium with inhibitor phosphinothricin (see paper)
68% identity, 99% coverage: 4:467/467 of query aligns to 5:468/468 of 1fpyA

query
sites
1fpyA

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P

P

V

V

M

M

L

L

A

A

Y

Y

S

S

A

A

R

R

N

N

R

R

S

S

A

A

S

S

I

I

R

R

I

I

P

P

Y

V

V

V

N

A

S

S

P

P

K

K

A

A

R

R

R
|
R

I

I

E
|
E

V

V

R
|
R

F

F

P

P

D

D

P

P

S

A

A

A

N

N

P

P

Y

Y

L

L

A

C

F

F

A

A

A

A

L

L

M

L

M

M

A

A

G

G

L

L

D

D

G

G

I

I

Q

K

N

N

K

K

I

I

H

H

P

P

G

G

D

E

A

P

M

M

D

D

K

K

D

N

L

L

Y

Y

D

D

L

L

P

P

K

P

E

E

E

E

A

A

L

K

S

E

I

I

P

P

T

Q

V

V

A

A

E

G

T

S

L

L

S

E

E

E

A

A

L

L

D

N

C

A

L

L

E

D

A

L

D

D

H

R

E

E

F

F

L

L

T

K

R

A

G

G

G

G

V

V

F

F

T

T

E

D

D

E

M

A

I

I

A

D

G

A

Y

Y

V

I

G

A

L

L

K

R

R

R

G

E

E

E

V

D

E

D

K

R

L

V

N

R

M

M

T

T

T

P

H

H

P

P

I

V

E

E

F

F

D

E

L

L

Y

Y

Y

Y

S

S

C

V

binding adenosine-5'-diphosphate: G127 (= G126), E129 (= E128), E207 (= E206), T223 (≠ V222), F225 (≠ A224), H271 (= H270), S273 (= S272), R355 (= R354), E357 (= E356)
binding manganese (ii) ion: E129 (= E128), E131 (= E130), E212 (= E211), E220 (= E219), H269 (= H268), E357 (= E356)
binding phosphinothricin: E131 (= E130), E212 (= E211), G265 (= G264), H269 (= H268), R321 (= R320), E327 (= E326), R359 (= R358)

1f1hA Crystal structure of glutamine synthetase from salmonella typhimurium with thallium ions (see paper)
68% identity, 99% coverage: 4:467/467 of query aligns to 5:468/468 of 1f1hA

query
sites
1f1hA

T

V

V

L

D

T

L

M

I

L

K

N

E

E

H

H

E

E

V

V

K

K

W

F

V

V

D

D

L

L

R

R

F

F

T

T

D

D

S

T

R

K

G

G

K

K

E

E

Q

Q

H

H

V

V

T

T

L

I

P

P

A

A

T

H

E

Q

V

V

D

N

E

A

D

E

F

F

F

F

A

E

D

E

G

G

K

K

M

M

F

F

D

D

G

G

S

S

S

S

I

I

A

G

G

G

W

W

K

K

G

G

I

I

N

N

E

E

S

S

D

D

M

M

I

V

L

L

M

M

P

P

D

D

D

A

E

S

T

T

S

A

V

V

L

I

D

D

P

P

F

F

T

F

E

A

E

D

T

S

T

T

V

L

N

I

I

I

T

R

C

C

N

D

I

I

V

L

E

E

P

P

S

G

T

T

M

L

Q

Q

G

G

Y

Y

E

D

R

R

D

D

P

P

R

R

S

S

V

I

A

A

R

K

R

R

A

A

E

E

E

D

Y

Y

L

L

K

R

S

A

T

T

G

G

I

I

A

A

D

D

G

T

A

V

L

L

F

F

G

G

P

P

E
|
E

P

P

E
|
E

F

F

F

F

V

L

F

F

D

D

S

D

V

I

K

R

W

F

N

G

V

A

D

S

M

I

Q

S

G

G

A

S

M

H

Y

V

H

A

I

I

H

D

S

D

E

I

E

E

A

G

A

A

W

W

V

N

S

S

G

S

E

T

D

K

F

Y

D

E

R

G

N

G

N

N

I

K

G

G

H

H

R

R

P

P

G

G

V

V

K

K

G

G

G

G

Y

Y

F

F

P

P

V

V

P

P

P

P

V

V

D

D

S

S

L

A

H

Q

D

D

L

I

R

R

G

S

A

E

M

M

C

C

A

L

A

V

M

M

E

E

S

Q

M

M

G

G

L

L

D

V

I

V

E
|
E

V

A

H

H

H
|
H

H

H

E
|
E

V

V

G

A

T

T

A

A

G

G

Q

Q

C

N

E
|
E

I

V

G

A

V
x
T

G

R

A
x
F

N

N

T

T

L

M

T

T

K

K

K

K

A

A

D

D

E

E

V

I

Q

Q

I

I

L

Y

K

K

Y

Y

C

V

V

V

H

H

N

N

V

V

A

A

H

H

A

R

Y

F

G

G

K

K

T

T

A

A

T

T

F

F

M

M

P

P

K

K

P

P

V

M

V

F

G

G

D

D

N

N

G

G

S

S

G

G

M

M

H
|
H

V

C

H
|
H

M

M

S
|
S

L

L

S

A

K

K

D

N

G

G

K

T

N

N

L

L

F

F

A

S

G

G

D

D

S

K

Y

Y

A

A

G

G

L

L

S

S

E

E

A

Q

A

A

L

L

Y

Y

Y

Y

I

I

G

G

G

G

V

V

I

I

K

K

H

H

A

A

K

K

A

A

I

I

N

N

A

A

F

L

T

A

N

N

S

P

S

T

T

T

N

N

S

S

Y

Y

K

K

R

R

L

L

V

V

P

P

G

G

F

Y

E

E

A

A

P

P

V

V

M

M

L

L

A

A

Y

Y

S

S

A

A

R

R

N

N

R

R

S

S

A

A

S

S

I

I

R

R

I

I

P

P

Y

V

V

V

N

A

S

S

P

P

K

K

A

A

R

R

R
|
R

I

I

E
|
E

V

V

R

R

F

F

P

P

D

D

P

P

S

A

A

A

N

N

P

P

Y

Y

L

L

A

C

F

F

A

A

A

A

L

L

M

L

M

M

A

A

G

G

L

L

D

D

G

G

I

I

Q

K

N

N

K

K

I

I

H

H

P

P

G

G

D

E

A

P

M

M

D

D

K

K

D

N

L

L

Y

Y

D

D

L

L

P

P

K

P

E

E

E

E

A

A

L

K

S

E

I

I

P

P

T

Q

V

V

A

A

E

G

T

S

L

L

S

E

E

E

A

A

L

L

D

N

C

A

L

L

E

D

A

L

D

D

H

R

E

E

F

F

L

L

T

K

R

A

G

G

G

G

V

V

F

F

T

T

E

D

D

E

M

A

I

I

A

D

G

A

Y

Y

V

I

G

A

L

L

K

R

R

R

G

E

E

E

V

D

E

D

K

R

L

V

N

R

M

M

T

T

T

P

H

H

P

P

I

V

E

E

F

F

D

E

L

L

Y

Y

Y

Y

S

S

C

V

binding adenosine-5'-diphosphate: E129 (= E128), E207 (= E206), H210 (= H209), E220 (= E219), T223 (≠ V222), F225 (≠ A224), H271 (= H270), S273 (= S272), R355 (= R354)
binding manganese (ii) ion: E129 (= E128), E131 (= E130), E212 (= E211), E220 (= E219), H269 (= H268), E357 (= E356)

2lgsA Feedback inhibition of fully unadenylylated glutamine synthetase from salmonella typhimurium by glycine, alanine, and serine (see paper)
64% identity, 99% coverage: 4:467/467 of query aligns to 5:445/445 of 2lgsA

query
sites
2lgsA

T

V

V

L

D

T

L

M

I

L

K

N

E

E

H

H

E

E

V

V

K

K

W

F

V

V

D

D

L

L

R

R

F

F

T

T

D

D

S

T

R

K

G

G

K

K

E

E

Q

Q

H

H

V

V

T

T

L

I

P

P

A

A

T

H

E

Q

V

V

D

N

E

A

D

E

F

F

F

F

A

E

D

E

G

G

K

K

M

M

F

F

D

D

G

G

S

S

S

S

I

I

A

G

G

G

W

W

K

-

G

-

I

-

N

-

E

-

S

-

D

D

M

M

I

V

L

L

M

M

P

P

D

D

D

A

E

S

T

T

S

A

V

V

L

I

D

D

P

P

F

F

T

F

E

A

E

D

T

S

T

T

V

L

N

I

I

I

T

R

C

C

N

D

I

I

V

L

E

E

P

P

S

G

T

T

M

L

Q

Q

G

G

Y

Y

E

D

R

R

D

D

P

P

R

R

S

S

V

I

A

A

R

K

R

R

A

A

E

E

E

D

Y

Y

L

L

K

R

S

A

T

T

G

G

I

I

A

A

D

D

G

T

A

V

L

L

F

F

G

G

P

P

E
|
E

P

P

E
|
E

F

F

F

F

V

L

F

F

D

D

S

D

V

I

K

R

W

F

N

G

V

A

D

S

M

I

Q

S

G

G

A

S

M

H

Y

V

H

A

I

I

H

D

S

D

E

I

E

E

A

G

A

A

W

W

V

N

S

S

G

S

E

T

D

K

F

Y

D

E

R

G

N

G

N

N

I

K

G

G

H

H

R

R

P

P

G

G

V

V

K

K

G

G

G

G

Y

Y

F

F

P

P

V

V

P

P

P

P

V

V

D

D

S

S

L

A

H

Q

D

D

L

I

R

R

G

S

A

E

M

M

C

C

A

L

A

V

M

M

E

E

S

Q

M

M

G

G

L

L

D

V

I

V

E

E

V

A

H

H

H

H

H

H

E
|
E

V

V

G

A

T

T

A

A

G

G

Q

Q

C

N

E
|
E

I

V

G

A

V

T

G

R

A

F

N

N

T

T

L

M

T

T

K

K

K

K

A

A

D

D

E

E

V

I

Q

Q

I

I

L

Y

K

K

Y

Y

C

V

V

V

H

H

N

N

V

V

A

A

H

H

A

R

Y

F

G

G

K

K

T

T

A

A

T

T

F

F

M

M

P

P

K

K

P

P

V

M

V

F

G

G

D

D

N
|
N

G
|
G

S

S

G
|
G

M

M

H
|
H

V

C

H

H

M

M

S

S

L

L

S

A

K

K

D

N

G

G

K

T

N

N

L

L

F

F

A

S

G

G

D

D

S

K

Y

Y

A

A

G

G

L

L

S

S

E

E

A

Q

A

A

L

L

Y

Y

Y

Y

I

I

G

G

G

G

V

V

I

I

K

K

H

H

A

A

K

K

A

A

I

I

N

N

A

A

F

L

T

A

N

N

S

P

S

T

T

T

N

N

S

S

Y

Y

K

K

R
|
R

L

L

V

V

P

P

G

-

F

-

E

-

A

-

P

P

V

V

M

M

L

L

A

A

Y

Y

S

S

A

A

R

R

N

N

R

R

S

S

A

A

S

S

I

I

R

R

I

I

P

P

Y

V

V

V

N

A

S

S

P

P

K

K

A

A

R

R

R

R

I

I

E
|
E

V

V

R
|
R

F

F

P

P

D

D

P

P

S

A

A

A

N

N

P

P

Y

Y

L

L

A

C

F

F

A

A

A

A

L

L

M

L

M

M

A

A

G

G

L

L

D

D

G

G

I

I

Q

K

N

N

K

K

I

I

H

H

P

P

G

G

D

E

A

P

M

M

D

D

K

-

D

-

L

-

Y

-

D

-

L

-

P

-

K

-

E

-

E

-

A

-

L

-

S

-

I

I

P

P

T

Q

V

V

A

A

E

G

T

S

L

L

S

E

E

E

A

A

L

L

D

N

C

A

L

L

E

D

A

L

D

D

H

R

E

E

F

F

L

L

T

K

R

A

G

G

G

G

V

V

F

F

T

T

E

D

D

E

M

A

I

I

A

D

G

A

Y

Y

V

I

G

A

L

L

K

R

R

R

G

E

E

E

V

D

E

D

K

R

L

V

N

R

M

M

T

T

T

P

H

H

P

P

I

V

E

E

F

F

D

E

L

L

Y

Y

Y

Y

S

S

C

V

binding glutamic acid: E125 (= E130), N258 (= N263), G259 (= G264), G261 (= G266), H263 (= H268), R315 (= R320), R349 (= R358)
binding manganese (ii) ion: E123 (= E128), E125 (= E130), E206 (= E211), E214 (= E219), H263 (= H268), E347 (= E356)

1lgrA Interactions of nucleotides with fully unadenylylated glutamine synthetase from salmonella typhimurium (see paper)
64% identity, 99% coverage: 4:467/467 of query aligns to 5:445/445 of 1lgrA

query
sites
1lgrA

T

V

V

L

D

T

L

M

I

L

K

N

E

E

H

H

E

E

V

V

K

K

W

F

V

V

D

D

L

L

R

R

F

F

T

T

D

D

S

T

R

K

G

G

K

K

E

E

Q

Q

H

H

V

V

T

T

L

I

P

P

A

A

T

H

E

Q

V

V

D

N

E

A

D

E

F

F

F

F

A

E

D

E

G

G

K

K

M

M

F

F

D

D

G

G

S

S

S

S

I

I

A

G

G

G

W

W

K

-

G

-

I

-

N

-

E

-

S

-

D

D

M

M

I

V

L

L

M

M

P

P

D

D

D

A

E

S

T

T

S

A

V

V

L

I

D

D

P

P

F

F

T

F

E

A

E

D

T

S

T

T

V

L

N

I

I

I

T

R

C

C

N

D

I

I

V

L

E

E

P

P

S

G

T

T

M

L

Q

Q

G

G

Y

Y

E

D

R

R

D

D

P

P

R

R

S

S

V

I

A

A

R

K

R

R

A

A

E

E

E

D

Y

Y

L

L

K

R

S

A

T

T

G

G

I

I

A

A

D

D

G

T

A

V

L

L

F

F

G

G

P

P

E
|
E

P

P

E
|
E

F

F

F

F

V

L

F

F

D

D

S

D

V

I

K

R

W

F

N

G

V

A

D

S

M

I

Q

S

G

G

A

S

M

H

Y

V

H

A

I

I

H

D

S

D

E

I

E

E

A

G

A

A

W

W

V

N

S

S

G

S

E

T

D

K

F

Y

D

E

R

G

N

G

N

N

I

K

G

G

H

H

R

R

P

P

G

G

V

V

K

K

G

G

G

G

Y

Y

F

F

P

P

V

V

P

P

P

P

V

V

D

D

S

S

L

A

H

Q

D

D

L

I

R

R

G

S

A

E

M

M

C

C

A

L

A

V

M

M

E

E

S

Q

M

M

G

G

L

L

D

V

I

V

E
|
E

V

A

H

H

H

H

H

H

E
|
E

V

V

G

A

T

T

A

A

G

G

Q

Q

C

N

E
|
E

I

V

G

A

V
x
T

G

R

A
x
F

N

N

T

T

L

M

T

T

K

K

K

K

A

A

D

D

E

E

V

I

Q

Q

I

I

L

Y

K

K

Y

Y

C

V

V

V

H

H

N

N

V

V

A

A

H

H

A

R

Y

F

G

G

K

K

T

T

A

A

T

T

F

F

M

M

P

P

K

K

P

P

V

M

V

F

G

G

D

D

N

N

G

G

S

S

G

G

M

M

H
|
H

V

C

H
|
H

M

M

S
|
S

L

L

S

A

K

K

D

N

G

G

K

T

N

N

L

L

F

F

A

S

G

G

D

D

S

K

Y

Y

A

A

G

G

L

L

S

S

E

E

A

Q

A

A

L

L

Y

Y

Y

Y

I

I

G

G

G

G

V

V

I

I

K

K

H

H

A

A

K

K

A

A

I

I

N

N

A

A

F

L

T

A

N

N

S

P

S

T

T

T

N

N

S

S

Y

Y

K

K

R

R

L

L

V

V

P

P

G

-

F

-

E

-

A

-

P

P

V

V

M

M

L

L

A

A

Y

Y

S

S

A

A

R

R

N

N

R

R

S

S

A

A

S

S

I

I

R

R

I

I

P

P

Y

V

V

V

N

A

S

S

P

P

K

K

A

A

R

R

R
|
R

I

I

E
|
E

V

V

R

R

F

F

P

P

D

D

P

P

S

A

A

A

N

N

P

P

Y

Y

L

L

A

C

F

F

A

A

A

A

L

L

M

L

M

M

A

A

G

G

L

L

D

D

G

G

I

I

Q

K

N

N

K

K

I

I

H

H

P

P

G

G

D

E

A

P

M

M

D

D

K

-

D

-

L

-

Y

-

D

-

L

-

P

-

K

-

E

-

E

-

A

-

L

-

S

-

I

I

P

P

T

Q

V

V

A

A

E

G

T

S

L

L

S

E

E

E

A

A

L

L

D

N

C

A

L

L

E

D

A

L

D

D

H

R

E

E

F

F

L

L

T

K

R

A

G

G

G

G

V

V

F

F

T

T

E

D

D

E

M

A

I

I

A

D

G

A

Y

Y

V

I

G

A

L

L

K

R

R

R

G

E

E

E

V

D

E

D

K

R

L

V

N

R

M

M

T

T

T

P

H

H

P

P

I

V

E

E

F

F

D

E

L

L

Y

Y

Y

Y

S

S

C

V

binding adenosine monophosphate: E201 (= E206), T217 (≠ V222), F219 (≠ A224), H265 (= H270), S267 (= S272), R345 (= R354)
binding manganese (ii) ion: E123 (= E128), E125 (= E130), E206 (= E211), E214 (= E219), H263 (= H268), E347 (= E356)

P77961 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I beta; GSI beta; EC 6.3.1.2 from Synechocystis sp. (strain PCC 6803 / Kazusa)
51% identity, 100% coverage: 2:467/467 of query aligns to 6:473/473 of P77961

query
sites
P77961

S

Q

K

E

T

V

V

L

D

K

L

W

I

I

K

Q

E

D

H

E

E

N

V

I

K

K

W

I

V

I

D

D

L

L

R

K

F

F

T

I

D

D

S

T

R

P

G

G

K

I

E

W

Q

Q

H

H

V

C

T

S

L

F

P

Y

A

Y

T

D

E

Q

V

L

D

D

E

E

D

N

F

S

F

F

A

T

D

E

G

G

K

I

M

P

F

F

D

D

G

G

S

S

S

S

I

I

A

R

G

G

W

W

K

K

G

A

I

I

N

N

E

E

S

S

D

D

M

M

I

C

L

M

M

V

P

P

D

D

D

P

E

N

T

T

S

A

V

T

L

I

D

D

P

P

F

F

T

C

E

K

E

E

T

P

T

T

V

L

N

S

I

M

T

I

C

C

N

S

I

I

V

K

E

E

P

P

S

R

T

T

M

G

Q

E

G

W

Y

Y

E

N

R

R

D

D

P

P

R

R

S

T

V

I

A

A

R

A

R

K

A

A

E

V

E

E

Y

Y

L

L

K

R

S

G

T

T

G

G

I

I

A

A

D

D

G

T

A

V

L

Y

F

F

G

G

P

P

E

E

P

A

E
|
E

F

F

F

F

V

L

F

F

D

D

S

D

V

I

K

R

W

F

N

G

V

Q

D

T

M

E

Q

N

G

S

A

S

M

Y

Y

Y

H

F

I

A

H

D

S

S

E

V

E

E

A

G

A

R

W

W

V

N

S

T

G

G

E

R

D

E

F

E

D

E

R

G

N

G

N

N

I

L

G

G

H

Y

R

K

P

P

G

G

V

Y

K

K

G

Q

G

G

Y

Y

F

F

P

P

V

V

P

A

P

P

V

T

D

D

S

T

L

A

H

Q

D

D

L

I

R

R

G

T

A

E

M

M

C

L

A

L

A

T

M

M

E

A

S

G

M

L

G

C

L

V

D

P

I

I

E

E

V

K

H

H

H

H

H

H

E
|
E

V

V

G

A

T

S

A

G

G

G

Q

Q

C

N

E
|
E

I

L

G

G

V

I

G

K

A

F

N

D

T

K

L

L

T

V

K

N

K

S

A

A

D

D

E

N

V

L

Q

M

I

I

L

Y

K

K

Y

Y

C

V

V

I

H

K

N

N

V

V

A

A

H

K

A

K

Y

Y

G

G

K

K

T

T

A

V

T

T

F

F

M

M

P

P

K

K

P

P

V

I

V

F

G

N

D

D

N

N

G

G

S

S

G

G

M

M

H

H

V

V

H

H

M

Q

S

S

L

L

S

W

K

K

D

D

G

G

K

Q

N

P

L

L

F

F

A

A

G

G

D

D

S

K

Y

Y

A

A

G

G

L

F

S

S

E

Q

A

M

A

G

L

L

Y

W

Y

Y

I

I

G

G

G

G

V

I

I

L

K

K

H

H

A

A

K

P

A

A

I

L

N

L

A

A

F

F

T

T

N

N

S

P

S

T

T

T

N

N

S

S

Y

Y

K

K

R

R

L

L

V

V

P

P

G

G

F

F

E

E

A

A

P

P

V

V

M

N

L

L

A

A

Y

Y

S

S

A

Q

R

G

N

N

R

R

S

S

A

A

S

S

I

V

R

R

I

I

P

P

Y

L

V

S

-

G

N

G

S

N

P

P

K

K

A

A

R

K

R

R

I

L

E
|
E

V

F

R

R

F

C

P

P

D

D

P

A

S

T

A

S

N

N

P

P

Y

Y

L

L

A

A

F

F

A

A

A

A

L

M

M

L

M

C

A

A

G

G

L

I

D

D

G

G

I

I

Q

K

N

N

K

Q

I

I

H

D

P

P

G

G

D

E

A

P

M

L

D

D

K

V

D

D

L

I

Y

Y

D

D

L

L

P

S

K

P

E

E

E

E

A

L

L

A

S

K

I

I

P

P

T

S

V

T

A

P

E

G

T

S

L

L

S

E

E

A

A

A

L

L

D

E

C

A

L

L

E

E

A

K

D

D

H

H

E

E

F

F

L

L

T

T

R

G

G

T

G

G

V

V

F

F

T

S

E

P

D

D

M

F

I

V

A

E

G

S

Y

W

V

I

G

E

L

Y

K

K

R

L

-

D

G

N

E

E

V

V

E

N

K

P

L

M

N

R

M

L

T

R

T

P

H

H

P

P

I

Y

E

E

F

F

D

S

L

L

Y

Y

Y

Y

S

D

C

C

E134 (= E130) binding Mg(2+)
E215 (= E211) binding Mg(2+)
E223 (= E219) binding Mg(2+)
E361 (= E356) binding Mn(2+)

A0R079 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I beta; GSI beta; EC 6.3.1.2 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
53% identity, 98% coverage: 7:465/467 of query aligns to 12:476/478 of A0R079

query
sites
A0R079

L

L

I

I

K
|
K

E

D

H

E

E

N

V

V

K

E

W

Y

V

V

D

D

L

I

R

R

F

F

T

C

D

D

S

L

R

P

G

G

K

V

E

V

Q

Q

H

H

V

F

T

S

L

I

P

P

A

A

T

S

E

A

V

F

D

D

E

E

D

S

F

V

F

F

A

E

D

D

G

G

K

L

M

A

F

F

D

D

G

G

S

S

S

S

I

V

A

R

G

G

W

F

K

Q

G

S

I

I

N

H

E

E

S

S

D

D

M

M

I

M

L

L

M

L

P

P

D

D

D

P

E

N

T

T

S

A

V

R

L

I

D

D

P

P

F

F

T

R

E

A

E

A

T

K

T

T

V

L

N

N

I

M

T

N

C

F

N

F

I

V

V

H

E

D

P

P

S

F

T

T

M

R

Q

E

G

A

Y

Y

E

S

R

R

D

D

P

P

R

R

S

N

V

V

A

A

R

R

R

K

A

A

E

E

E

N

Y

Y

L

L

K

A

S

S

T

T

G

G

I

I

A

A

D

D

G

T

A

A

L

F

F

F

G

G

P

A

E

E

P

A

E

E

F

F

F

Y

V

I

F

F

D

D

S

S

V

V

K

S

W

F

N

D

V

S

D

K

M

I

Q

N

G

G

A

T

M

F

Y

Y

H

E

I

V

H

D

S

S

E

E

E

S

A

G

A

W

W

W

V

N

S

T

G

G

E

E

D

P

F

F

D

E

R

S

N

D

-

G

-

S

-

A

N

N

I

R

G

G

H

Y

R

K

P

V

G

R

V

P

K

K

G

G

G

G

Y

Y

F

F

P

P

V

V

P

A

P

P

V

Y

D

D

S

H

L

Y

H

V

D

D

L

L

R

R

G

D

A

Q

M

M

C

A

A

T

A

N

M

L

E

Q

S

N

M

A

G

G

L

F

D

T

I

L

E

E

V

R

H

G

H

H

H

H

E

E

V

V

G

G

T

T

A

A

G

G

Q

Q

C

A

E

E

I

I

G

N

V

Y

G

K

A

F

N

N

T

T

L

L

T

L

K

A

K

A

A

A

D

D

E

D

V

V

Q

L

I

L

L

F

K

K

Y

Y

C

I

V

I

H

K

N

N

V

T

A

A

H

W

A

Q

Y

A

G

G

K

K

T

T

A

V

T

T

F

F

M

M

P

P

K

K

P

P

V

L

V

F

G

G

D

D

N

N

G

G

S

S

G

G

M

M

H

H

V

A

H

H

M

Q

S

S

L

L

S

W

K

K

D

D

G

G

K

Q

N

P

L

L

F

F

A

H

G

D

D

E

S

S

-

G

Y

Y

A

A

G

G

L

L

S

S

E

D

A

I

A

A

L

R

Y

H

Y

Y

I

I

G

G

G

G

V

I

I

L

K

H

H

H

A

A

K

P

A

S

I

L

N

L

A

A

F

F

T

T

N

N

S

P

S

T

T

V

N

N

S

S

Y

Y

K

K

R

R

L

L

V

V

P

P

G

G

F

Y

E

E

A

A

P

P

V

I

M

N

L

L

A

V

Y

Y

S

S

A

Q

R

R

N

N

R

R

S

S

A

A

S

C

I

V

R

R

I

I

P

P

Y

I

V

T

-

G

N

N

S

N

P

P

K

K

A

A

R

K

R

R

I

L

E

E

V

F

R

R

F

C

P

P

D

D

P

S

S

S

A

G

N

N

P

P

Y

Y

L

L

A

A

F

F

A

A

A

A

L

M

M

L

M

M

A

A

G

G

L

I

D

D

G

G

I

I

Q

K

N

K

K

K

I

I

H

E

P

P

G

L

D

Q

A

P

M

V

D

D

K

K

D

D

L

L

Y

Y

D

E

L

L

P

P

K

P

E

D

E

E

A

A

L

A

S

A

I

I

P

P

T

Q

V

A

A

P

E

T

T

S

L

L

S

S

E

A

A

V

L

I

D

D

C

K

L

L

E

E

A

E

D

D

H

H

E

E

F

Y

L

L

T

T

R

E

G

G

G

G

V

V

F

F

T

T

E

E

D

D

M

L

I

I

A

E

G

T

Y

W

V

I

G

S

L

Y

K

K

R

R

-

E

G

N

E

E

V

I

E

M

K

P

L

I

N

Q

M

I

T

R

T

P

H

H

P

P

I

Y

E

E

F

F

D

S

L

L

Y

Y

Y

Y

K14 (= K9) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

3ng0A Crystal structure of glutamine synthetase from synechocystis sp. Pcc 6803
50% identity, 100% coverage: 2:467/467 of query aligns to 4:465/465 of 3ng0A

query
sites
3ng0A

S

Q

K

E

T

V

V

L

D

K

L

W

I

I

K

Q

E

D

H

E

E

N

V

I

K

K

W

I

V

I

D

D

L

L

R

K

F

F

T

I

D

D

S

T

R

P

G

G

K

I

E

W

Q

Q

H

H

V

C

T

S

L

F

P

Y

A

Y

T

D

E

Q

V

L

D

D

E

E

D

N

F

S

F

F

A

T

D

E

G

G

K

I

M

P

F

F

D
|
D

G

G

S

S

S

S

I

I

A

R

G

G

W

W

K

K

G

A

I

I

N

N

E

E

S

S

D

D

M

M

I

C

L

M

M

V

P

P

D

D

D

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E

N

T

T

S

A

V

T

L

I

D

D

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P

F

F

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C

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K

E

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T

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N

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I

M

T

I

C

C

N

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Q

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Y

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A

A

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A

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A

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A

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Y

L

L

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G

T

T

G

G

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A

A

D

D

G

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A

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x
Y

F

F

G

G

P

P

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|
E

P

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|
E

F

F

F

F

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L

F

F

D

D

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D

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V

E

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A

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A

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W

W

V

N

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G

G

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D

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F

E

D

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G

N

G

N

N

I

L

G

G

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Y

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K

P

P

G

G

V

Y

K

K

G

Q

G

G

Y

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V

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A

P

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D

D

S

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L

A

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D

D

L

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R

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A

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M

M

C

L

A

L

A

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M

M

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A

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A

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F

G

G

L

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D

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I

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x
K

H

H

H

H

H

H

E
|
E

V

V

G

A

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S

A

G

G

G

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Q

C

N

E
|
E

I

L

G

G

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x
I

G

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A
x
F

N

D

T

K

L

L

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N

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A

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D

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N

V

L

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Y

Y

C

V

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N

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Y

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G

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K

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T

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F

F

M

M

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K

K

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I

V

F

G

N

D

D

N

N

G

G

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S

G

G

M

M

H
|
H

V

V

H
|
H

M

Q

S
|
S

L

L

S

W

K

K

D

D

G

G

K

Q

N

P

L

L

F

F

A

A

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G

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D

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K

Y

Y

A

A

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G

L

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A

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A

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L

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L

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N

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Y

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K

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L

L

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N

N

R
|
R

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A

A

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|
R

I

I

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P

Y

L

V

S

-

G

N

G

S

N

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P

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K

A

A

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K

R
|
R

I

L

E
|
E

V

F

R
|
R

F

C

P

P

D

D

P

A

S

T

A

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N

N

P

P

Y

Y

L

L

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A

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F

A

A

A

A

L

M

M

L

M

C

A

A

G

G

L

I

D

D

G

G

I

I

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K

N

N

K

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A

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D

K

V

D

D

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L

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A

A

A

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A

L

L

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F

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V

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F

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D

D

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A

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Y

W

V

I

G

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L

Y

K

K

-

L

R

D

G

N

E

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V

V

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N

K

P

L

M

N

R

M

L

T

R

T

P

H

H

P

P

I

Y

E

E

F

F

D

S

L

L

Y

Y

Y

Y

S

D

C

C

active site: D51 (= D49), E130 (= E128), E132 (= E130), E213 (= E211), E221 (= E219), H270 (= H268), R340 (= R338), E359 (= E356), R361 (= R358)
binding phosphoaminophosphonic acid-adenylate ester: Y126 (≠ L124), E130 (= E128), K209 (≠ V207), I224 (≠ V222), F226 (≠ A224), H272 (= H270), S274 (= S272), R340 (= R338), R345 (= R343), R357 (= R354)
binding manganese (ii) ion: E130 (= E128), E132 (= E130), E213 (= E211), E221 (= E219), H270 (= H268), E359 (= E356), R361 (= R358)

2whiA Crystal structure of mycobacterium tuberculosis glutamine synthetase in complex with a purine analogue inhibitor and l-methionine-s- sulfoximine phosphate. (see paper)
52% identity, 98% coverage: 7:465/467 of query aligns to 10:474/476 of 2whiA

query
sites
2whiA

L

L

I

A

K

K

E

D

H

E

E

K

V

V

K

E

W

Y

V

V

D

D

L

V

R

R

F

F

T

C

D

D

S

L

R

P

G

G

K

I

E

M

Q

Q

H

H

V

F

T

T

L

I

P

P

A

A

T

S

E

A

V

F

D

D

E

K

D

S

F

V

F

F

A

D

D

D

G

G

K

L

M

A

F

F

D
|
D

G

G

S

S

S

S

I

I

A

R

G

G

W

F

K

Q

G

S

I

I

N

H

E

E

S

S

D

D

M

M

I

L

L

L

M

L

P

P

D

D

D

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E

T

T

S

A

V

R

L

I

D

D

P

P

F

F

T

R

E

A

E

A

T

K

T

T

V

L

N

N

I

I

T

N

C

F

N

F

I

V

V

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P

S

F

T

T

M

L

Q

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Y

Y

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R

D

D

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P

R

R

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N

V

I

A

A

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R

R

K

A

A

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E

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N

Y

Y

L

L

K

I

S

S

T

T

G

G

I

I

A

A

D

D

G

T

A

A

L
x
Y

F

F

G
|
G

P

A

E
|
E

P

A

E
|
E

F

F

F

Y

V

I

F

F

D

D

S

S

V

V

K

S

W

F

N

D

V

S

D

R

M

A

Q

N

G

G

A

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M

F

Y

Y

H

E

I

V

H

D

S

A

E

I

E

S

A

G

A

W

W

W

V

N

S

T

G

G

E

A

D

A

F

T

D

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A

N

D

-

G

-

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-

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N

N

I

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G

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Y

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K

G

G

G

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Y

Y

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D

D

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M

M

C

L

A

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A

N

M

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N

M

S

G

G

L

F

D

I

I

L

E

E

V

K

H

G

H

H

H

H

E
|
E

V

V

G

G

T

S

A

G

G

G

Q

Q

C

A

E
|
E

I

I

G

N

V

Y

G

Q

A
x
F

N

N

T

S

L

L

T

L

K

H

K

A

A

A

D

D

E

D

V

M

Q

Q

I

L

L

Y

K

K

Y

Y

C

I

V

I

H

K

N

N

V

T

A

A

H

W

A

Q

Y

N

G

G

K

K

T

T

A

V

T

T

F

F

M

M

P

P

K

K

P

P

V

L

V

F

G

G

D

D

N

N

G
|
G

S

S

G

G

M

M

H
|
H

V

C

H
|
H

M

Q

S
|
S

L

L

S
x
W

K

K

D

D

G

G

K

A

N

P

L

L

F

M

A

Y

G

D

D

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S

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-

G

Y

Y

A

A

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G

L

L

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S

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D

A

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A

A

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Y

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Y

Y

I

I

G

G

G

G

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L

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L

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H

H

H

A

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A

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N

L

A

A

F

F

T

T

N

N

S

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S

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T

V

N

N

S

S

Y

Y

K

K

R
|
R

L

L

V

V

P

P

G

G

F

Y

E
|
E

A

A

P

P

V

I

M

N

L

L

A

V

Y

Y

S

S

A

Q

R

R

N

N

R
|
R

S

S

A

A

S

C

I

V

R
|
R

I

I

P

P

Y

I

V

T

N

G

S

S

-

N

P

P

K
|
K

A

A

R

K

R
|
R

I

L

E
|
E

V

F

R
|
R

F