SitesBLAST
Comparing GFF443 PGA1_c04540 putative d-galactonate dehydratase to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3rcyF Crystal structure of mandelate racemase/muconate lactonizing enzyme- like protein from roseovarius sp. Tm1035
76% identity, 96% coverage: 1:395/410 of query aligns to 1:395/397 of 3rcyF
- active site: K165 (= K165), D167 (= D167), R175 (= R175), G208 (= G208), H210 (= H210), E234 (= E234), G259 (= G259), E260 (= E260), Q281 (= Q281), A283 (= A283), H310 (= H310), A313 (= A313), L332 (= L332), E335 (= E335)
- binding magnesium ion: E234 (= E234), E260 (= E260)
- binding alpha-D-ribofuranose: R85 (= R85), P86 (= P86), P239 (= P239), A266 (= A266), E267 (= E267)
4e5tA Crystal structure of a putative mandelate racemase/muconate lactonizing enzyme (target psi-200750) from labrenzia alexandrii dfl- 11
54% identity, 96% coverage: 1:392/410 of query aligns to 1:389/392 of 4e5tA
3sjnA Crystal structure of enolase spea_3858 (target efi-500646) from shewanella pealeana with magnesium bound
30% identity, 91% coverage: 1:373/410 of query aligns to 2:366/373 of 3sjnA
- active site: S46 (≠ A42), L49 (≠ V45), T139 (≠ Y130), K165 (= K165), G167 (≠ D167), M171 (= M179), D198 (≠ G208), A200 (≠ H210), E225 (= E234), I247 (≠ V256), G250 (= G259), E251 (= E260), S252 (≠ R261), Q272 (= Q281), D274 (≠ A283), H301 (= H310), G302 (≠ L311), F303 (≠ Y312), M325 (≠ L332), E326 (≠ M333), Q329 (≠ T336), S331 (≠ E338)
- binding magnesium ion: D198 (≠ G208), E225 (= E234), E251 (= E260)
2o56A Crystal structure of a member of the enolase superfamily from salmonella typhimurium
30% identity, 93% coverage: 1:380/410 of query aligns to 2:389/392 of 2o56A
- active site: K165 (= K165), D167 (= D167), M171 (vs. gap), L186 (≠ A181), E214 (≠ G208), H216 (= H210), E240 (= E234), G265 (= G259), E266 (= E260), Q287 (= Q281), D289 (≠ A283), H316 (= H310), E341 (= E335), A346 (≠ P340)
- binding magnesium ion: E214 (≠ G208), E240 (= E234), E266 (= E260)
4e6mA Crystal structure of putative dehydratase protein from salmonella enterica subsp. Enterica serovar typhimurium (salmonella typhimurium)
31% identity, 86% coverage: 24:375/410 of query aligns to 27:393/401 of 4e6mA
- active site: L45 (≠ Y41), G48 (≠ S44), K169 (= K165), D171 (= D167), I175 (vs. gap), E223 (≠ G208), H225 (= H210), E249 (= E234), G274 (= G259), E275 (= E260), R276 (= R261), Q296 (= Q281), D298 (≠ A283), H325 (= H310), C327 (≠ Y312), E350 (= E335), A355 (≠ L345)
- binding magnesium ion: E223 (≠ G208), E249 (= E234), E275 (= E260)
3rraB Crystal structure of enolase prk14017 (target efi-500653) from ralstonia pickettii 12j with magnesium bound
32% identity, 86% coverage: 23:373/410 of query aligns to 16:351/379 of 3rraB
- active site: I35 (≠ C40), R38 (≠ A43), Y118 (= Y128), K145 (= K165), N147 (≠ D167), E151 (≠ T173), D184 (≠ G208), H186 (= H210), E210 (= E234), G235 (= G259), E236 (= E260), R237 (= R261), Q257 (= Q281), D259 (≠ A283), H286 (= H310), P288 (≠ Y312), E311 (= E335)
- binding magnesium ion: D184 (≠ G208), E210 (= E234), E236 (= E260)
2qq6B Crystal structure of mandelate racemase/muconate lactonizing enzyme- like protein from rubrobacter xylanophilus dsm 9941
30% identity, 84% coverage: 36:381/410 of query aligns to 31:396/396 of 2qq6B
- active site: P37 (≠ A42), G79 (≠ R85), D124 (vs. gap), K166 (= K165), D168 (= D167), D213 (≠ G208), H215 (= H210), E239 (= E234), G264 (= G259), E265 (= E260), M286 (≠ Q281), D288 (≠ A283), H315 (= H310), N316 (≠ L311), E340 (= E335), D345 (≠ E338)
- binding magnesium ion: D213 (≠ G208), E239 (= E234), E265 (= E260), H315 (= H310)
2ox4C Crystal structure of putative dehydratase from zymomonas mobilis zm4
29% identity, 88% coverage: 20:380/410 of query aligns to 19:389/397 of 2ox4C
- active site: K165 (= K165), D167 (= D167), E214 (≠ G208), H216 (= H210), E240 (= E234), G265 (= G259), E266 (= E260), Q287 (= Q281), D289 (≠ A283), H316 (= H310), E341 (= E335)
- binding magnesium ion: E214 (≠ G208), E240 (= E234), E266 (= E260)
4hpnA Crystal structure of a proposed galactarolactone cycloisomerase from agrobacterium tumefaciens, target efi-500704, with bound ca, ordered loops (see paper)
30% identity, 86% coverage: 21:372/410 of query aligns to 29:368/378 of 4hpnA
- active site: G50 (= G46), R53 (≠ A49), T134 (≠ L131), K164 (= K165), K166 (≠ P180), D194 (≠ G208), N196 (≠ H210), E220 (= E234), G245 (= G259), E246 (= E260), T247 (≠ R261), Q267 (= Q281), D269 (≠ A283), H296 (= H310), V297 (≠ L311), W298 (≠ Y312), R320 (≠ M333), E329 (vs. gap), F330 (vs. gap), H334 (vs. gap)
- binding calcium ion: D194 (≠ G208), D209 (≠ Q223), E220 (= E234), G237 (≠ R251), E246 (= E260)
Sites not aligning to the query:
3ozmA Crystal structure of enolase superfamily member from bordetella bronchiseptica complexed with mg, m-xylarate and l-lyxarate
30% identity, 88% coverage: 21:382/410 of query aligns to 32:377/386 of 3ozmA
- active site: G53 (= G46), D56 (vs. gap), S143 (≠ Y130), K170 (= K165), K172 (≠ R175), D200 (≠ G208), N202 (≠ H210), E226 (= E234), G252 (= G259), E253 (= E260), N254 (≠ R261), Q274 (= Q281), D276 (≠ A283), H303 (= H310), T304 (≠ L311), F305 (≠ Y312), E328 (= E335), I331 (≠ T336), H333 (≠ E338)
- binding D-xylaric acid: Y146 (≠ F142), K170 (= K165), K172 (≠ R175), D200 (≠ G208), N202 (≠ H210), E253 (= E260), H303 (= H310), F305 (≠ Y312), E328 (= E335)
- binding magnesium ion: D200 (≠ G208), E226 (= E234), E253 (= E260)
Sites not aligning to the query:
3op2A Crystal structure of putative mandelate racemase from bordetella bronchiseptica rb50 complexed with 2-oxoglutarate/phosphate
29% identity, 88% coverage: 21:382/410 of query aligns to 32:372/375 of 3op2A
- active site: G53 (= G46), D56 (vs. gap), S138 (≠ Y130), K165 (= K165), K167 (≠ R175), D195 (≠ G208), N197 (≠ H210), E221 (= E234), G247 (= G259), E248 (= E260), N249 (≠ R261), Q269 (= Q281), D271 (≠ A283), H298 (= H310), T299 (≠ L311), F300 (≠ Y312), E323 (= E335), I326 (≠ T336), H328 (≠ E338)
- binding 2-oxoglutaric acid: K165 (= K165), K167 (≠ R175), D195 (≠ G208), E248 (= E260), H298 (= H310), E323 (= E335)
- binding magnesium ion: D195 (≠ G208), E221 (= E234), E248 (= E260)
Sites not aligning to the query:
3ozmD Crystal structure of enolase superfamily member from bordetella bronchiseptica complexed with mg, m-xylarate and l-lyxarate
30% identity, 88% coverage: 21:382/410 of query aligns to 32:377/381 of 3ozmD
- active site: G53 (= G46), D56 (vs. gap), S143 (≠ Y130), K170 (= K165), K172 (≠ R175), D200 (≠ G208), N202 (≠ H210), E226 (= E234), G252 (= G259), E253 (= E260), N254 (≠ R261), Q274 (= Q281), D276 (≠ A283), H303 (= H310), T304 (≠ L311), F305 (≠ Y312), E328 (= E335), I331 (≠ T336), H333 (≠ E338)
- binding L-arabinaric acid: K172 (≠ R175), D200 (≠ G208), N202 (≠ H210), E253 (= E260), H303 (= H310), F305 (≠ Y312), E328 (= E335)
- binding magnesium ion: D200 (≠ G208), E226 (= E234), E253 (= E260)
Sites not aligning to the query:
3bjsB Crystal structure of a member of enolase superfamily from polaromonas sp. Js666
32% identity, 88% coverage: 21:380/410 of query aligns to 28:369/376 of 3bjsB
- active site: P49 (≠ A42), S52 (≠ G46), L93 (vs. gap), G136 (≠ H137), K164 (= K165), R166 (= R175), D194 (≠ G208), N196 (≠ H210), E220 (= E234), G246 (= G259), E247 (= E260), N248 (≠ R261), Q268 (= Q281), D270 (≠ A283), H297 (= H310), S298 (≠ L311), S299 (≠ Y312), E322 (= E335), C324 (≠ I337), K327 (≠ P340)
- binding magnesium ion: D194 (≠ G208), E220 (= E234), E247 (= E260)
Sites not aligning to the query:
3gy1B Crystal structure of putative mandelate racemase/muconate lactonizing protein from clostridium beijerinckii ncimb 8052
25% identity, 85% coverage: 24:372/410 of query aligns to 23:358/388 of 3gy1B
- active site: R152 (≠ K165), Q154 (≠ D167), D191 (≠ G208), H193 (= H210), E217 (= E234), G242 (= G259), E243 (= E260), R264 (≠ Q281), H266 (≠ A283), H293 (= H310), E320 (= E335)
- binding magnesium ion: D191 (≠ G208), E217 (= E234), E243 (= E260)
3v3wA Crystal structure of an enolase from the soil bacterium cellvibrio japonicus (target efi-502161) with bound mg and glycerol
25% identity, 92% coverage: 1:379/410 of query aligns to 1:379/397 of 3v3wA
- active site: L36 (≠ A42), R39 (≠ V45), H122 (≠ Y130), K144 (≠ T162), R147 (≠ K165), Q149 (≠ D167), Y159 (≠ M174), E179 (vs. gap), D205 (≠ G208), H207 (= H210), E231 (= E234), G256 (= G259), E257 (= E260), V258 (≠ R261), R278 (≠ Q281), T280 (≠ A283), F306 (≠ P309), H307 (= H310), G308 (vs. gap), A309 (vs. gap), E334 (= E335)
- binding magnesium ion: D205 (≠ G208), E231 (= E234), E257 (= E260)
Sites not aligning to the query:
B3PDB1 D-galactonate dehydratase family member RspA; D-mannonate dehydratase; Starvation sensing protein RspA homolog; EC 4.2.1.-; EC 4.2.1.8 from Cellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subsp. cellulosa) (see paper)
25% identity, 92% coverage: 1:379/410 of query aligns to 1:384/402 of B3PDB1
- D210 (≠ G208) binding
- E236 (= E234) binding
- E262 (= E260) binding
4gmeC Crystal structure of mannonate dehydratase (target efi-502209) from caulobacter crescentus cb15 complexed with magnesium and d-mannonate
29% identity, 92% coverage: 1:379/410 of query aligns to 2:385/403 of 4gmeC
- active site: L37 (≠ S44), R40 (≠ P47), D211 (≠ G208), H213 (= H210), E237 (= E234), G262 (= G259), E263 (= E260), I264 (≠ R261), T286 (≠ A283), H313 (= H310), A315 (vs. gap), E340 (= E335)
- binding d-mannonic acid: N38 (≠ V45), Y160 (= Y172), D211 (≠ G208), H213 (= H210), E237 (= E234), E263 (= E260), H313 (= H310), D317 (≠ Y312), E340 (= E335)
- binding magnesium ion: D211 (≠ G208), E237 (= E234), E263 (= E260)
Sites not aligning to the query:
4gmeA Crystal structure of mannonate dehydratase (target efi-502209) from caulobacter crescentus cb15 complexed with magnesium and d-mannonate
29% identity, 92% coverage: 1:379/410 of query aligns to 2:385/403 of 4gmeA
- active site: L37 (≠ S44), R40 (≠ P47), T119 (≠ R126), R148 (≠ K165), Q150 (≠ D167), Y160 (= Y172), D211 (≠ G208), H213 (= H210), E237 (= E234), G262 (= G259), E263 (= E260), I264 (≠ R261), R284 (≠ Q281), T286 (≠ A283), H313 (= H310), A315 (vs. gap), E340 (= E335)
- binding carbonate ion: R148 (≠ K165), Y160 (= Y172), D211 (≠ G208), E263 (= E260), E340 (= E335)
- binding magnesium ion: D211 (≠ G208), E237 (= E234), E263 (= E260)
Sites not aligning to the query:
Q9AAR4 D-mannonate dehydratase CC0532; ManD; EC 4.2.1.8 from Caulobacter vibrioides (strain ATCC 19089 / CB15) (Caulobacter crescentus) (see paper)
29% identity, 92% coverage: 1:379/410 of query aligns to 2:385/403 of Q9AAR4
- D211 (≠ G208) binding
- E237 (= E234) binding
- E263 (= E260) binding
A6M2W4 D-galactonate dehydratase family member Cbei_4837 from Clostridium beijerinckii (strain ATCC 51743 / NCIMB 8052) (Clostridium acetobutylicum) (see paper)
25% identity, 85% coverage: 24:372/410 of query aligns to 22:372/399 of A6M2W4
- D205 (≠ G208) binding
- E231 (= E234) binding
- E257 (= E260) binding
Query Sequence
>GFF443 PGA1_c04540 putative d-galactonate dehydratase
MKLQDLDIIVTAPPAPGWGGRYWILVKVTTDTGITGWGECYAASVGPDAMTHVIRDVFER
HMQGMNPENIEWMFRRAYSSGFTQRPDLSVMGAFSGLEIACWDILGKDRDRPVHALIGGR
MNDRLRAYTYLYPLPHHDIDAFWNTPELAAESAIAAVEKGYTAVKFDPAGPYTMRGGHMP
AQSDITQSVAFCRAIREAVGDRADLLFGTHGQFTPAGAIRLGQALESYEPLWFEEPTPPD
LVADMARVADRVRIPVATGERLTTKAEFAAILRAGAAEILQPALGRSGGIWETKKIAAIA
EVFGAQMAPHLYAGPVEWAANIQLAASIPNLLMIETIETPFHTALIKQGITVEDGFVIPS
DTPGLGIEVDEDLARANPFIGDDLHLNMQDAPCNYAEGNSFVGGAPPMED
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory