Comparing GFF4493 FitnessBrowser__WCS417:GFF4493 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P23533 Phosphoenolpyruvate-protein phosphotransferase; Phosphotransferase system, enzyme I; EC 2.7.3.9 from Staphylococcus carnosus (strain TM300) (see paper)
35% identity, 67% coverage: 277:832/836 of query aligns to 5:569/573 of P23533
2wqdA Crystal structure of enzyme i of the phosphoenolpyruvate:sugar phosphotransferase system in the dephosphorylated state (see paper)
34% identity, 67% coverage: 276:832/836 of query aligns to 3:569/570 of 2wqdA
P08839 Phosphoenolpyruvate-protein phosphotransferase; Phosphotransferase system, enzyme I; EC 2.7.3.9 from Escherichia coli (strain K12) (see 2 papers)
36% identity, 64% coverage: 276:814/836 of query aligns to 1:550/575 of P08839
2hwgA Structure of phosphorylated enzyme i of the phosphoenolpyruvate:sugar phosphotransferase system (see paper)
36% identity, 64% coverage: 277:814/836 of query aligns to 1:549/572 of 2hwgA
2xz9A Crystal structure from the phosphoenolpyruvate-binding domain of enzyme i in complex with pyruvate from the thermoanaerobacter tengcongensis pep-sugar phosphotransferase system (pts) (see paper)
42% identity, 36% coverage: 529:830/836 of query aligns to 5:311/317 of 2xz9A
2xz7A Crystal structure of the phosphoenolpyruvate-binding domain of enzyme i in complex with phosphoenolpyruvate from the thermoanaerobacter tengcongensis pep-sugar phosphotransferase system (pts) (see paper)
42% identity, 36% coverage: 529:830/836 of query aligns to 12:318/324 of 2xz7A
5lu4A C4-type pyruvate phosphate dikinase: conformational intermediate of central domain in the swiveling mechanism (see paper)
29% identity, 45% coverage: 415:794/836 of query aligns to 416:840/850 of 5lu4A
Sites not aligning to the query:
5jvjB C4-type pyruvate phosphate dikinase: different conformational states of the nucleotide binding domain in the dimer (see paper)
28% identity, 45% coverage: 415:794/836 of query aligns to 343:785/797 of 5jvjB
Sites not aligning to the query:
5jvlA C4-type pyruvate phospate dikinase: nucleotide binding domain with bound atp analogue (see paper)
27% identity, 45% coverage: 415:794/836 of query aligns to 416:862/874 of 5jvlA
Sites not aligning to the query:
Q39735 Pyruvate, phosphate dikinase, chloroplastic; Cold-sensitive pyruvate, orthophosphate dikinase; Pyruvate, orthophosphate dikinase; EC 2.7.9.1 from Flaveria bidentis (Coastal plain yellowtops) (Ethulia bidentis) (see 2 papers)
27% identity, 45% coverage: 415:794/836 of query aligns to 495:941/953 of Q39735
Sites not aligning to the query:
O23404 Pyruvate, phosphate dikinase 1, chloroplastic; Pyruvate, orthophosphate dikinase 1; EC 2.7.9.1 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
29% identity, 47% coverage: 406:794/836 of query aligns to 496:950/963 of O23404
1vbgA Pyruvate phosphate dikinase from maize (see paper)
29% identity, 47% coverage: 406:794/836 of query aligns to 407:862/874 of 1vbgA
Sites not aligning to the query:
P11155 Pyruvate, phosphate dikinase 1, chloroplastic; Pyruvate, orthophosphate dikinase 1; EC 2.7.9.1 from Zea mays (Maize) (see 7 papers)
28% identity, 47% coverage: 406:794/836 of query aligns to 480:935/947 of P11155
Sites not aligning to the query:
5jvlB C4-type pyruvate phospate dikinase: nucleotide binding domain with bound atp analogue (see paper)
27% identity, 41% coverage: 456:794/836 of query aligns to 123:508/520 of 5jvlB
Sites not aligning to the query:
P69783 PTS system glucose-specific EIIA component; EIIA-Glc; EIII-Glc; Glucose-specific phosphotransferase enzyme IIA component from Escherichia coli (strain K12) (see 6 papers)
42% identity, 17% coverage: 12:153/836 of query aligns to 25:167/169 of P69783
Sites not aligning to the query:
1glcF Cation promoted association (cpa) of a regulatory and target protein is controlled by phosphorylation (see paper)
42% identity, 17% coverage: 12:153/836 of query aligns to 17:159/161 of 1glcF
1vbhA Pyruvate phosphate dikinase with bound mg-pep from maize (see paper)
28% identity, 47% coverage: 406:794/836 of query aligns to 398:850/862 of 1vbhA
Sites not aligning to the query:
1o2fA Complex of enzyme iiaglc and iibglc phosphocarrier protein hpr from escherichia coli nmr, restrained regularized mean structure (see paper)
42% identity, 17% coverage: 12:153/836 of query aligns to 6:148/150 of 1o2fA
P09323 PTS system N-acetylglucosamine-specific EIICBA component; EIICBA-Nag; EII-Nag; EC 2.7.1.193 from Escherichia coli (strain K12) (see paper)
42% identity, 15% coverage: 10:135/836 of query aligns to 501:626/648 of P09323
Sites not aligning to the query:
1kc7A Pyruvate phosphate dikinase with bound mg-phosphonopyruvate (see paper)
26% identity, 44% coverage: 431:794/836 of query aligns to 429:858/872 of 1kc7A
Sites not aligning to the query:
>GFF4493 FitnessBrowser__WCS417:GFF4493
MSYNNNELTLSAPLSGPVLTLGNVPDEVFASGAMGDGIAIDPLNDCLYAPCDGVIIHVAR
TGHALTIRAENGAEVLMHVGIDTVELNGEGFALLVKDGARVSKGQALVQFDLDRIARQCK
SLVSLIILTNGERFELRAVAGQKVKVGEPLLQIVARSAAAVQTAVDNSEADVSASVRITH
RGGLHARPAALIRKTAQDFSSQAQLHFGDKSASCDSLIGLMGLGIGEGDEVRVTCRGKDA
EAALQALVAALSAVIKEEHHVPVVAPPRRAHTEAGVLQGVCAAPGLVCGPLFRLTGIELP
ADVGNHSADEQLQHLDTALEQVRSEIRSTLAHARQRKNVEEEDIFAAHLALLEDPNLLEA
ATRSIEQGSAATHAWRDAIQAQCAVLLALGKPLFAERANDLRDLQQRVLRALLGEAWHFE
LPAGSIVSAHELTPSDLLQLSAQHAVGICMAEGGATSHVAILARGKGLPCVVALGAEVLD
VPQGQRVVLDAVNGRLELEPTEARHAEVHQIRDAQKLRRQQQQAQAQEPARTTDGVSIEV
AANVASSAEAQVAFENGADGVGLLRTEFLFVDRRTAPDEQEQRHAYQAVLDAMGDKSVII
RTIDVGGDKQLDYLPLPVEANPVLGLRGIRMAQVRPELLDQQLRALLQVSPLERCRILLP
MVSEVDELLQIRQRLDELCVELELTQRPELGVMIEVPAAALMAEHLAKHADFLSIGTNDL
SQYTLAMDRDHAGLAARVDALHPALLRLIAQTCAGATKHGRWVGVCGALASDPLATPVLV
GLGVSELSVSPPQIAEIKDRVRHLDAAQCRQLSQGLLDLSSAKAVRQACQHHWPLS
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SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.
Lawrence Berkeley National Laboratory