SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF4553 FitnessBrowser__WCS417:GFF4553 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

1co6A Crystal structure of ferrocytochrome c2 from rhodopseudomonas viridis (see paper)
47% identity, 81% coverage: 23:123/125 of query aligns to 2:101/107 of 1co6A

query
sites
1co6A

D

D

A

A

E

A

A

S

G

G

G

E

K

Q

L

V

F

F

T

-

K

K

T

Q

C
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C

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C
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C

H
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H

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binding heme c: C13 (= C35), C16 (= C38), H17 (= H39), P29 (= P51), S39 (≠ A61), Y47 (= Y69), S48 (= S70), N51 (≠ M73), W58 (= W80), F63 (≠ L85), I67 (= I89), T77 (= T99), K78 (≠ R100), M79 (= M101), F81 (= F103)

P00083 Cytochrome c2 from Blastochloris viridis (Rhodopseudomonas viridis) (see 2 papers)
46% identity, 77% coverage: 28:123/125 of query aligns to 26:121/127 of P00083

query
sites
P00083

G
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G

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x
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x
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K

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C33 (= C35) binding covalent
C36 (= C38) binding covalent
H37 (= H39) binding axial binding residue
M99 (= M101) binding axial binding residue

Sites not aligning to the query:

1:20 signal peptide
21 modified: Pyrrolidone carboxylic acid
21:127 modified: mature protein, Cytochrome c2

P00069 Cytochrome c from Guizotia abyssinica (Niger) (Ramtilla)
45% identity, 80% coverage: 21:120/125 of query aligns to 8:107/111 of P00069

query
sites
P00069

A

A

G

G

D

D

A

A

E

K

A

A

G

G

G

E

K

K

L

I

F

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C

C

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C

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G

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B

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K

K80 (= K93) modified: N6,N6,N6-trimethyllysine
K94 (≠ S107) modified: N6,N6,N6-trimethyllysine

Sites not aligning to the query:

1 modified: N-acetylalanine

P00015 Cytochrome c, testis-specific from Mus musculus (Mouse) (see paper)
42% identity, 79% coverage: 22:120/125 of query aligns to 2:100/105 of P00015

query
sites
P00015

G
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G

D

D

A

A

E

E

A

A

G

G

G

K

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I

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K

G2 (= G22) modified: N-acetylglycine
C15 (= C35) binding covalent
C18 (= C38) binding covalent

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

2aiuA Crystal structure of mouse testicular cytochromE C at 1.6 angstrom (see paper)
42% identity, 79% coverage: 22:120/125 of query aligns to 1:99/104 of 2aiuA

query
sites
2aiuA

G

G

D

D

A

A

E

E

A

A

G

G

G

K

K

K

L

I

F

F

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C
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C

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A

G
x
Q

C
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C

H
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H

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E

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G

G

A

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A

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G
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G

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Q

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A

A

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Y

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x
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D

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x
N

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binding protoporphyrin ix containing fe: C14 (= C35), Q16 (≠ G37), C17 (= C38), H18 (= H39), P30 (= P51), L35 (≠ I56), G41 (= G62), Y48 (= Y69), T49 (≠ S70), N52 (≠ M73), W59 (= W80), Y67 (= Y88), L68 (≠ I89), T78 (= T99), K79 (≠ R100), M80 (= M101), F82 (= F103)

P00014 Cytochrome c from Macropus giganteus (Eastern gray kangaroo) (see paper)
43% identity, 79% coverage: 22:120/125 of query aligns to 2:100/105 of P00014

query
sites
P00014

G
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G

D

D

A

V

E

E

A

K

G

G

G

K

K

K

L

I

F

F

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Q

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C
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C

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A

G

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C

H

H

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I

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G

E

E

K

G

G

A

G

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P

Q

N

L

L

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N

G

G

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I

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P

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A

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G

G

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A

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Y

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D

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K

G2 (= G22) modified: N-acetylglycine
C15 (= C35) binding covalent
C18 (= C38) binding covalent

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

P68519 Cytochrome c from Crotalus viridis viridis (Prairie rattlesnake) (see paper)
41% identity, 79% coverage: 22:120/125 of query aligns to 2:100/105 of P68519

query
sites
P68519

G
|
G

D

D

A

V

E

E

A

K

G

G

G

K

K

K

L

I

F

F

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M

T

K

C

C

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G

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C

C

H

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E

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D

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L

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A

A

Y

Y

L

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K

G2 (= G22) modified: N-acetylglycine

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

P68518 Cytochrome c from Crotalus atrox (Western diamondback rattlesnake) (see paper)
41% identity, 79% coverage: 22:120/125 of query aligns to 2:100/105 of P68518

query
sites
P68518

G
|
G

D

D

A

V

E

E

A

K

G

G

G

K

K

K

L

I

F

F

T

S

K

M

T

K

C

C

G

G

G

T

C

C

H

H

S

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I

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G

E

E

E

G

G

A

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G

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N

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N

H

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A

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A

A

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D

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Y

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Y

Y

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D

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A

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M

N

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N

N

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G

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D

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L

I

A

A

Y

Y

L

L

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K

G2 (= G22) modified: N-acetylglycine

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

P68517 Cytochrome c from Crotalus adamanteus (Eastern diamondback rattlesnake) (see paper)
41% identity, 79% coverage: 22:120/125 of query aligns to 2:100/105 of P68517

query
sites
P68517

G
|
G

D

D

A

V

E

E

A

K

G

G

G

K

K

K

L

I

F

F

T

S

K

M

T

K

C

C

G

G

G

T

C

C

H

H

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G

E

E

E

G

G

A

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G

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P

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N

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H

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A

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K

G2 (= G22) modified: N-acetylglycine

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

P00018 Cytochrome c from Dromaius novaehollandiae (Emu) (see paper)
42% identity, 79% coverage: 22:120/125 of query aligns to 2:100/105 of P00018

query
sites
P00018

G
|
G

D

D

A

I

E

E

A

K

G

G

G

K

K

K

L

I

F

F

T

V

K

Q

T

K

C
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C

G

S

G

Q

C
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C

H

H

S

T

I

V

G

E

E

K

G

G

A

G

R

K

G

H

G

K

F

T

G

G

P

P

Q

N

L

L

N

N

G

G

I

L

I

F

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G

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R

P

K

A

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G

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A

A

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D

G

Y

F

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Y

S

T

D

D

A

A

M

N

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K

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N

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P

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Y

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K

G2 (= G22) modified: N-acetylglycine
C15 (= C35) binding covalent
C18 (= C38) binding covalent

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

P00008 Cytochrome c from Oryctolagus cuniculus (Rabbit) (see paper)
41% identity, 79% coverage: 22:120/125 of query aligns to 2:100/105 of P00008

query
sites
P00008

G
|
G

D

D

A

V

E

E

A

K

G

G

G

K

K

K

L

I

F

F

T

V

K

Q

T

K

C
|
C

G

A

G

Q

C
|
C

H

H

S

T

I

V

G

E

E

K

G

G

A

G

R

K

G

H

G

K

F

T

G

G

P

P

Q

N

L

L

N

H

G

G

I

L

I

F

G

G

R

R

P

K

A

T

G

G

T

Q

T

A

A

V

D

G

Y

F

Q

S

Y

Y

S

T

D

D

A

A

M

N

K

K

N

N

S

K

G

G

V

I

V

T

W

W

T

G

R

E

D

D

K

T

L

L

A

M

A

E

Y

Y

I

L

E

E

A

N

P

P

K

K

K

K

V

Y

V

I

S

P

G

G

T

T

R

K

M

M

I

I

F

F

W

A

G

G

I

I

S

K

D

K

Q

K

E

D

K

E

I

R

D

A

N

D

I

L

L

I

A

A

Y

Y

L

L

E

K

G2 (= G22) modified: N-acetylglycine
C15 (= C35) binding covalent
C18 (= C38) binding covalent

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

P00004 Cytochrome c from Equus caballus (Horse) (see 2 papers)
40% identity, 79% coverage: 22:120/125 of query aligns to 2:100/105 of P00004

query
sites
P00004

G
|
G

D

D

A

V

E

E

A

K

G

G

G

K

K

K

L

I

F

F

T

V

K

Q

T

K

C

C

G

A

G

Q

C

C

H
|
H

S

T

I

V

G

E

E

K

G

G

A

G

R

K

G

H

G

K

F

T

G

G

P

P

Q

N

L

L

N

H

G

G

I

L

I

F

G

G

R

R

P

K

A

T

G

G

T

Q

T

A

A

P

D

G

Y

F

Q

T

Y

Y

S

T

D

D

A

A

M

N

K

K

N

N

S

K

G

G

V

I

V

T

W

W

T

K

R

E

D

E

K

T

L

L

A

M

A

E

Y

Y

I

L

E

E

A

N

P

P

K

K

K

K

V

Y

V

I

S

P

G

G

T

T

R

K

M

M

I

I

F

F

W

A

G

G

I

I

S

K

D

K

Q

K

E

T

K

E

I

R

D

E

N

D

I

L

L

I

A

A

Y

Y

L

L

E

K

G2 (= G22) modified: N-acetylglycine
H19 (= H39) binding axial binding residue

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

P62898 Cytochrome c, somatic from Rattus norvegicus (Rat) (see paper)
42% identity, 79% coverage: 22:120/125 of query aligns to 2:100/105 of P62898

query
sites
P62898

G
|
G

D

D

A

V

E

E

A

K

G

G

G

K

K

K

L

I

F

F

T

V

K

Q

T

K

C

C

G

A

G

Q

C

C

H

H

S

T

I

V

G

E

E

K

G

G

A

G

R

K

G

H

G

K

F

T

G

G

P

P

Q

N

L

L

N

H

G

G

I

L

I

F

G

G

R

R

P

K

A

T

G

G

T

Q

T

A

A

A

D

G

Y

F

Q

S

Y

Y

S

T

D

D

A

A

M

N

K

K

N

N

S

K

G

G

V

I

V

T

W

W

T

G

R

E

D

D

K

T

L

L

A

M

A

E

Y

Y

I

L

E

E

A

N

P

P

K

K

K

K

V

Y

V

I

S

P

G

G

T

T

R

K

M

M

I

I

F

F

W

A

G

G

I

I

S

K

D

K

Q

K

E

G

K

E

I

R

D

A

N

D

I

L

L

I

A

A

Y

Y

L

L

E

K

G2 (= G22) modified: N-acetylglycine

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

P62897 Cytochrome c, somatic from Mus musculus (Mouse) (see paper)
42% identity, 79% coverage: 22:120/125 of query aligns to 2:100/105 of P62897

query
sites
P62897

G
|
G

D

D

A

V

E

E

A

K

G

G

G

K

K

K

L

I

F

F

T

V

K

Q

T

K

C

C

G

A

G

Q

C

C

H

H

S

T

I

V

G

E

E

K

G

G

A

G

R

K

G

H

G

K

F

T

G

G

P

P

Q

N

L

L

N

H

G

G

I

L

I

F

G

G

R

R

P

K

A

T

G

G

T

Q

T

A

A

A

D

G

Y

F

Q

S

Y

Y

S

T

D

D

A

A

M

N

K

K

N

N

S

K

G

G

V

I

V

T

W

W

T

G

R

E

D

D

K

T

L

L

A

M

A

E

Y

Y

I

L

E

E

A

N

P

P

K

K

K

K

V

Y

V

I

S

P

G

G

T

T

R

K

M

M

I

I

F

F

W

A

G

G

I

I

S

K

D

K

Q

K

E

G

K

E

I

R

D

A

N

D

I

L

L

I

A

A

Y

Y

L

L

E

K

G2 (= G22) modified: N-acetylglycine

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

6suvAaA Cytochrome c
40% identity, 79% coverage: 22:120/125 of query aligns to 1:99/104 of 6suvAaA

query
sites
6suvAaA

G

G

D

D

A

V

E
|
E

A

K

G

G

G
x
K

K
|
K

L

I

F

F

T
x
V

K

Q

T

K

C
|
C

G

A

G

Q

C
|
C

H
|
H

S

T

I

V

G

E

E

K

G

G

A

G

R

K

G

H

G

K

F
x
T

G
|
G

P
|
P

Q

N

L

L

N

H

G

G

I
x
L

I

F

G

G

R

R

P

K

A
x
T

G
|
G

T

Q

T

A

A

P

D

G

Y

F

Q

T

Y
|
Y

S
x
T

D

D

A

A

M
x
N

K

K

N

N

S

K

G

G

V

I

V

T

W
|
W

T

K

R

E

D

E

K

T

L

L

A

M

A

E

Y
|
Y

I
x
L

E

E

A

N

P

P

K
|
K

K
|
K

V

Y

V

I

S
x
P

G

G

T
|
T

R
x
K

M
|
M

I

I

F
|
F

W

A

G

G

I

I

S
x
K

D
x
K

Q
x
K

E
x
T

K
x
E

I

R

D

E

N

D

I

L

L

I

A

A

Y

Y

L

L

E

K

binding heme c: C14 (= C35), C17 (= C38), H18 (= H39), T28 (≠ F49), G29 (= G50), P30 (= P51), L35 (≠ I56), T40 (≠ A61), G41 (= G62), Y48 (= Y69), T49 (≠ S70), N52 (≠ M73), W59 (= W80), Y67 (= Y88), L68 (≠ I89), T78 (= T99), K79 (≠ R100), M80 (= M101), F82 (= F103)
binding octa-anionic calixarene: E4 (= E25), K7 (≠ G28), K8 (= K29), V11 (≠ T32), K72 (= K93), K73 (= K94), P76 (≠ S97), K86 (≠ S107), K87 (≠ D108), K88 (≠ Q109), T89 (≠ E110), E90 (≠ K111)

4rszA The x-ray structure of the primary adduct formed in the reaction between cisplatin and cytochromE C (see paper)
40% identity, 79% coverage: 22:120/125 of query aligns to 1:99/104 of 4rszA

query
sites
4rszA

G

G

D

D

A

V

E

E

A

K

G

G

G

K

K

K

L

I

F

F

T

V

K

Q

T
x
K

C
|
C

G

A

G

Q

C
|
C

H
|
H

S

T

I

V

G

E

E

K

G

G

A

G

R

K

G

H

G

K

F

T

G
|
G

P
|
P

Q

N

L

L

N

H

G

G

I

L

I

F

G

G

R

R

P

K

A

T

G

G

T

Q

T

A

A

P

D

G

Y

F

Q

T

Y
|
Y

S
x
T

D

D

A

A

M
x
N

K

K

N

N

S

K

G

G

V

I

V

T

W
|
W

T

K

R
x
E

D

E

K

T

L

L

A
x
M

A

E

Y
|
Y

I

L

E

E

A

N

P

P

K

K

K

K

V

Y

V

I

S

P

G

G

T
|
T

R
x
K

M
|
M

I
|
I

F

F

W

A

G

G

I

I

S

K

D

K

Q

K

E

T

K

E

I

R

D
x
E

N

D

I

L

L

I

A

A

Y

Y

L

L

E

K

binding Cisplatin: E61 (≠ R82), M65 (≠ A86), E92 (≠ D113)
binding heme c: K13 (≠ T34), C14 (= C35), C17 (= C38), H18 (= H39), G29 (= G50), P30 (= P51), Y48 (= Y69), T49 (≠ S70), N52 (≠ M73), W59 (= W80), Y67 (= Y88), T78 (= T99), K79 (≠ R100), M80 (= M101), I81 (= I102)

1wejF Igg1 fab fragment (of e8 antibody) complexed with horse cytochromE C at 1.8 a resolution (see paper)
40% identity, 79% coverage: 22:120/125 of query aligns to 1:99/104 of 1wejF

query
sites
1wejF

G

G

D

D

A

V

E

E

A

K

G

G

G

K

K

K

L

I

F

F

T

V

K

Q

T
x
K

C
|
C

G

A

G

Q

C
|
C

H
|
H

S

T

I

V

G

E

E

K

G

G

A

G

R

K

G

H

G

K

F

T

G
|
G

P
|
P

Q

N

L

L

N

H

G

G

I

L

I

F

G

G

R

R

P

K

A

T

G
|
G

T

Q

T

A

A

P

D

G

Y

F

Q

T

Y
|
Y

S
x
T

D

D

A

A

M
x
N

K

K

N

N

S

K

G

G

V

I

V

T

W
|
W

T

K

R

E

D

E

K

T

L

L

A

M

A

E

Y
|
Y

I
x
L

E

E

A

N

P

P

K

K

K

K

V

Y

V

I

S

P

G

G

T
|
T

R
x
K

M
|
M

I

I

F
|
F

W

A

G

G

I

I

S

K

D

K

Q

K

E

T

K

E

I

R

D

E

N

D

I

L

L

I

A

A

Y

Y

L

L

E

K

binding protoporphyrin ix containing fe: K13 (≠ T34), C14 (= C35), C17 (= C38), H18 (= H39), G29 (= G50), P30 (= P51), G41 (= G62), Y48 (= Y69), T49 (≠ S70), N52 (≠ M73), W59 (= W80), Y67 (= Y88), L68 (≠ I89), T78 (= T99), K79 (≠ R100), M80 (= M101), F82 (= F103)

1m60A Solution structure of zinc-substituted cytochromE C (see paper)
40% identity, 79% coverage: 22:120/125 of query aligns to 1:99/104 of 1m60A

query
sites
1m60A

G

G

D

D

A

V

E

E

A

K

G

G

G

K

K

K

L

I

F

F

T

V

K

Q

T
x
K

C
|
C

G

A

G

Q

C
|
C

H
|
H

S

T

I

V

G

E

E

K

G

G

A

G

R

K

G

H

G

K

F
x
T

G

G

P

P

Q

N

L
|
L

N

H

G

G

I

L

I

F

G

G

R

R

P

K

A
x
T

G
|
G

T

Q

T

A

A

P

D

G

Y

F

Q

T

Y
|
Y

S
x
T

D

D

A

A

M
x
N

K

K

N

N

S

K

G

G

V

I

V

T

W
|
W

T

K

R

E

D

E

K

T

L

L

A

M

A

E

Y
|
Y

I
x
L

E

E

A

N

P

P

K

K

K

K

V

Y

V

I

S

P

G

G

T

T

R
x
K

M
|
M

I

I

F
|
F

W

A

G

G

I

I

S

K

D

K

Q

K

E

T

K

E

I

R

D

E

N

D

I
x
L

L

I

A

A

Y

Y

L

L

E

K

binding zinc substituted heme c: K13 (≠ T34), C14 (= C35), C17 (= C38), H18 (= H39), T28 (≠ F49), L32 (= L53), T40 (≠ A61), G41 (= G62), Y48 (= Y69), T49 (≠ S70), N52 (≠ M73), W59 (= W80), Y67 (= Y88), L68 (≠ I89), K79 (≠ R100), M80 (= M101), F82 (= F103), L94 (≠ I115)

1fi7A Solution structure of the imidazole complex of cytochromE C (see paper)
40% identity, 79% coverage: 22:120/125 of query aligns to 1:99/104 of 1fi7A

query
sites
1fi7A

G

G

D

D

A

V

E

E

A

K

G

G

G

K

K

K

L

I

F

F

T

V

K

Q

T

K

C
|
C

G

A

G

Q

C
|
C

H
|
H

S

T

I

V

G

E

E

K

G

G

A

G

R

K

G

H

G

K

F
x
T

G
|
G

P
|
P

Q

N

L
|
L

N

H

G

G

I

L

I

F

G

G

R

R

P

K

A

T

G

G

T

Q

T

A

A

P

D

G

Y

F

Q

T

Y

Y

S

T

D

D

A

A

M
x
N

K

K

N

N

S

K

G

G

V

I

V

T

W
|
W

T

K

R

E

D

E

K

T

L
|
L

A

M

A

E

Y
|
Y

I
x
L

E

E

A

N

P

P

K

K

K

K

V

Y

V

I

S

P

G

G

T

T

R
x
K

M

M

I

I

F

F

W

A

G

G

I

I

S

K

D

K

Q

K

E

T

K

E

I

R

D

E

N

D

I

L

L

I

A

A

Y

Y

L

L

E

K

binding heme c: C14 (= C35), C17 (= C38), H18 (= H39), T28 (≠ F49), G29 (= G50), P30 (= P51), L32 (= L53), N52 (≠ M73), W59 (= W80), L64 (= L85), L68 (≠ I89), K79 (≠ R100)
binding imidazole: Y67 (= Y88), K79 (≠ R100)

1akkA Solution structure of oxidized horse heart cytochromE C, nmr, minimized average structure (see paper)
40% identity, 79% coverage: 22:120/125 of query aligns to 1:99/104 of 1akkA

query
sites
1akkA

G

G

D

D

A

V

E

E

A

K

G

G

G

K

K

K

L

I

F

F

T

V

K

Q

T

K

C
|
C

G

A

G

Q

C
|
C

H
|
H

S

T

I

V

G

E

E

K

G

G

A

G

R

K

G

H

G

K

F
x
T

G

G

P

P

Q

N

L

L

N

H

G

G

I

L

I

F

G

G

R

R

P

K

A
x
T

G

G

T

Q

T

A

A

P

D

G

Y

F

Q

T

Y
|
Y

S
x
T

D

D

A

A

M
x
N

K

K

N

N

S

K

G

G

V

I

V

T

W
|
W

T

K

R

E

D

E

K

T

L

L

A

M

A

E

Y
|
Y

I

L

E

E

A

N

P

P

K

K

K

K

V

Y

V

I

S

P

G

G

T
|
T

R
x
K

M
|
M

I

I

F
|
F

W

A

G

G

I

I

S

K

D

K

Q

K

E

T

K

E

I

R

D

E

N

D

I
x
L

L

I

A

A

Y

Y

L

L

E

K

binding heme c: C14 (= C35), C17 (= C38), H18 (= H39), T28 (≠ F49), T40 (≠ A61), Y48 (= Y69), T49 (≠ S70), N52 (≠ M73), W59 (= W80), Y67 (= Y88), T78 (= T99), K79 (≠ R100), M80 (= M101), F82 (= F103), L94 (≠ I115)

Query Sequence

>GFF4553 FitnessBrowser__WCS417:GFF4553
MKYRTLITLALVFTAGPAFAAGDAEAGGKLFTKTCGGCHSIGEGARGGFGPQLNGIIGRP
AGTTADYQYSDAMKNSGVVWTRDKLAAYIEAPKKVVSGTRMIFWGISDQEKIDNILAYLE
TFQPK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory