SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF460 FitnessBrowser__psRCH2:GFF460 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4husA Crystal structure of streptogramin group a antibiotic acetyltransferase vata from staphylococcus aureus in complex with virginiamycin m1 (see paper)
36% identity, 68% coverage: 40:198/234 of query aligns to 16:194/212 of 4husA

query
sites
4husA

N

N

V
x
L

R

Q

L

F

G

I

D

K

V

P

A

T

I

I

K

T

A

N

R

E

D

N

L

I

R

L

I

V

G

G

A

E

Y

Y

T

S

Y
|
Y

I

Y

R
x
D

S

S

G

K

L

R

L

G

Q

E

S

S

V

F

S

E

E

D

-

Q

-

V

-

L

-

Y

-

H

-

Y

-

E

-

V

-

I

-

G

-

D

-

K

-

L

-

I

I

I

G

G

R

R

F

F

C

C

S

S

I

I

G

G

Q

P

E

G

V

T

Q

T

I

F

G

I

L

M

S

N

S

G

D

A

A

N

H

H

P

R

L

M

Y

D

W

G

L

-

T

S

T

T

H

Y

P

P

V

F

H

H

D

L

I

F

A

R

A

M

G

G

-

W

-

E

-

K

-

Y

-

M

-

P

-

S

L

L

K

K

Y

D

D

L

T

P

Q

L

K

K

A

G

H

D

A

I

R

E

I

I

G

G

H

N

D

D

V

V

W

W

I

I

G

G

A

R

G

D

V

V

K

T

I

I

L

M

S

P

G

G

V

V

T

K

V

I

G

G

D

D

G

G

A

A

V

I

I

I

A

A

T

A

G

E

A

A

V

V

T

T

R

K

H

N

V

V

A

A

P

P

Y

Y

E

S

I

I

V

V

G

G

N

N

P

P

A

L

K

K

H

F

I

I

K

R

W

K

R

R

F

F

E

S

D

D

S

G

I

V

L

I

R

-

D

E

E

E

I

W

H

L

K

A

S

L

A

Q

W

W

N

N

L

L

Q

D

F

M

E

K

Q

I

L

I

-

N

K

E

A

N

M

L

P

P

F

F

binding virginiamycin m1: L17 (≠ V41), Y36 (= Y60), D38 (≠ R62)

4hurA Crystal structure of streptogramin group a antibiotic acetyltransferase vata from staphylococcus aureus in complex with acetyl coenzyme a (see paper)
36% identity, 68% coverage: 40:198/234 of query aligns to 16:194/211 of 4hurA

query
sites
4hurA

N

N

V

L

R

Q

L

F

G

I

D

K

V

P

A

T

I

I

K

T

A

N

R

E

D

N

L

I

R

L

I

V

G

G

A

E

Y

Y

T

S

Y

Y

I

Y

R

D

S

S

G

K

L

R

L

G

Q

E

S

S

V

F

S

E

E

D

-

Q

-

V

-

L

-
x
Y

-

H

-

Y

-

E

-

V

-

I

-

G

-

D

-

K

-

L

-

I

I

I

G

G

R

R

F

F

C

C

S
|
S

I
|
I

G
|
G

Q

P

E

G

V

T

Q

T

I

F

G

I

L

M

S

N

S

G

D
x
A

A
x
N

H

H

P

R

L

M

Y

D

W

G

L

-

T

S

T

T

H

Y

P

P

V

F

H

H

D

L

I

F

A

R

A

M

G

G

-

W

-

E

-

K

-

Y

-

M

-

P

-

S

L

L

K

K

Y

D

D

L

T

P

Q

L

K
|
K

A

G

H

D

A

I

R

E

I

I

G

G

H

N

D

D

V

V

W
|
W

I
|
I

G
|
G

A
x
R

G

D

V

V

K

T

I

I

L
x
M

S

P

G

G

V

V

T

K

V

I

G

G

D

D

G

G

A

A

V

I

I

I

A
|
A

T
x
A

G

E

A

A

V

V

T
|
T

R

K

H

N

V

V

A

A

P

P

Y

Y

E

S

I

I

V

V

G
|
G

G

G

N

N

P
|
P

A

L

K

K

H

F

I
|
I

K
x
R

W

K

R

R

F

F

E

S

D

D

S

G

I

V

L

I

R

-

D

E

E

E

I

W

H

L

K

A

S

L

A

Q

W

W

N

N

L

L

Q

D

F

M

E

K

Q

I

L

I

-

N

K

E

A

N

M

L

P

P

F

F

binding acetyl coenzyme *a: Y51 (vs. gap), S67 (= S77), I68 (= I78), G69 (= G79), A79 (≠ D89), N80 (≠ A90), K110 (= K114), W120 (= W124), I121 (= I125), G122 (= G126), R123 (≠ A127), M128 (≠ L132), A140 (= A144), A141 (≠ T145), T146 (= T150), G156 (= G160), P159 (= P163), I163 (= I167), R164 (≠ K168)

6x3jA Crystal structure of streptogramin a acetyltransferase vata from staphylococcus aureus in complex with streptogramin analog f0224 (46) (see paper)
36% identity, 68% coverage: 40:198/234 of query aligns to 16:194/206 of 6x3jA

query
sites
6x3jA

N

N

V

L

R

Q

L

F

G

I

D

K

V

P

A

T

I

I

K

T

A

N

R

E

D

N

L

I

R

L

I

V

G

G

A

E

Y

Y

T

S

Y

Y

I

Y

R

D

S

S

G

K

L

R

L

G

Q

E

S

S

V

F

S

E

E

D

-

Q

-

V

-

L

-
x
Y

-

H

-
x
Y

-

E

-
x
V

-

I

-

G

-

D

-

K

-

L

-

I

I

I

G

G

R

R

F

F

C

C

S

S

I

I

G
|
G

Q

P

E

G

V

T

Q

T

I

F

G

I

L

M

S

N

S

G

D
x
A

A

N

H
|
H

P

R

L

M

Y

D

W

G

L

-

T

S

T

T

H

Y

P

P

V

F

H
|
H

D
x
L

I

F

A

R

A

M

G

G

-

W

-

E

-

K

-

Y

-
x
M

-
x
P

-

S

L

L

K

K

Y

D

D
x
L

T

P

Q

L

K

K

A

G

H

D

A

I

R

E

I

I

G

G

H

N

D

D

V

V

W
|
W

I

I

G
|
G

A

R

G

D

V

V

K

T

I

I

L

M

S

P

G

G

V

V

T

K

V

I

G

G

D

D

G

G

A

A

V

I

I

I

A

A

T

A

G

E

A

A

V

V

T

T

R

K

H

N

V

V

A

A

P

P

Y

Y

E

S

I

I

V

V

G

G

N

N

P

P

A

L

K

K

H

F

I

I

K

R

W

K

R

R

F

F

E

S

D

D

S

G

I

V

L

I

R

-

D

E

E

E

I

W

H

L

K

A

S

L

A

Q

W

W

N

N

L

L

Q

D

F

M

E

K

Q

I

L

I

-

N

K

E

A

N

M

L

P

P

F

F

binding (2R)-2-[(3S,4R,5E,10E,12E,14S,16R,26aR)-16-fluoro-14-hydroxy-4,12-dimethyl-1,7,22-trioxo-4,7,8,9,14,15,16,17,24,25,26,26a-dodecahydro-1H,3H,22H-21,18-(azeno)pyrrolo[2,1-c][1,8,4,19]dioxadiazacyclotetracosin-3-yl]propyl isoquinolin-3-ylcarbamate: Y51 (vs. gap), Y53 (vs. gap), V55 (vs. gap), H91 (= H102), L92 (≠ D103), M101 (vs. gap), P102 (vs. gap), L107 (≠ D111)
binding thioacetic acid s-{2-[3-(2-hydroxy-3,3-dimethyl-4-phosphonooxy-butyrylamino)-propionylamino]-ethyl} ester: G69 (= G79), A79 (≠ D89), H81 (= H91), W120 (= W124), G122 (= G126)

6x3cA Crystal structure of streptogramin a acetyltransferase vata from staphylococcus aureus in complex with streptogramin analog f1037 (47) (see paper)
36% identity, 68% coverage: 40:198/234 of query aligns to 16:194/207 of 6x3cA

query
sites
6x3cA

N

N

V
x
L

R

Q

L

F

G

I

D

K

V

P

A

T

I

I

K

T

A

N

R

E

D

N

L

I

R

L

I

V

G

G

A

E

Y

Y

T

S

Y
|
Y

I

Y

R

D

S

S

G

K

L

R

L

G

Q

E

S

S

V

F

S

E

E

D

-

Q

-

V

-

L

-
x
Y

-

H

-
x
Y

-

E

-

V

-

I

-

G

-

D

-

K

-

L

-

I

I

I

G

G

R

R

F

F

C

C

S

S

I

I

G
|
G

Q

P

E

G

V

T

Q

T

I

F

G

I

L

M

S

N

S
x
G

D

A

A
x
N

H
|
H

P

R

L

M

Y

D

W

G

L

-

T

S

T

T

H

Y

P

P

V

F

H
|
H

D
x
L

I

F

A

R

A

M

G

G

-

W

-

E

-

K

-

Y

-
x
M

-
x
P

-

S

L
|
L

K

K

Y

D

D

L

T

P

Q

L

K

K

A

G

H

D

A

I

R

E

I

I

G

G

H

N

D

D

V

V

W
|
W

I

I

G
|
G

A
x
R

G

D

V

V

K

T

I

I

L
x
M

S

P

G

G

V

V

T

K

V

I

G

G

D

D

G

G

A

A

V

I

I

I

A
|
A

T
x
A

G

E

A

A

V

V

T

T

R

K

H

N

V

V

A

A

P

P

Y

Y

E

S

I

I

V

V

G

G

N

N

P

P

A

L

K

K

H

F

I

I

K

R

W

K

R

R

F

F

E

S

D

D

S

G

I

V

L

I

R

-

D

E

E

E

I

W

H

L

K

A

S

L

A

Q

W

W

N

N

L

L

Q

D

F

M

E

K

Q

I

L

I

-

N

K

E

A

N

M

L

P

P

F

F

binding (3R,4R,5E,10E,12E,14S,16R,26aR)-16-fluoro-14-hydroxy-12-methyl-3-(propan-2-yl)-4-(prop-2-en-1-yl)-3,4,8,9,14,15,16,17,24,25,26,26a-dodecahydro-1H,7H,22H-21,18-(azeno)pyrrolo[2,1-c][1,8,4,19]dioxadiazacyclotetracosine-1,7,22-trione: L17 (≠ V41), Y36 (= Y60), Y53 (vs. gap), G78 (≠ S88), N80 (≠ A90), H81 (= H91), H91 (= H102), L92 (≠ D103), M101 (vs. gap), P102 (vs. gap), L104 (= L108)
binding thioacetic acid s-{2-[3-(2-hydroxy-3,3-dimethyl-4-phosphonooxy-butyrylamino)-propionylamino]-ethyl} ester: Y51 (vs. gap), G69 (= G79), N80 (≠ A90), H81 (= H91), W120 (= W124), G122 (= G126), R123 (≠ A127), M128 (≠ L132), A140 (= A144), A141 (≠ T145)

6x3cE Crystal structure of streptogramin a acetyltransferase vata from staphylococcus aureus in complex with streptogramin analog f1037 (47) (see paper)
36% identity, 68% coverage: 40:198/234 of query aligns to 16:194/203 of 6x3cE

query
sites
6x3cE

N

N

V

L

R

Q

L

F

G

I

D

K

V

P

A

T

I

I

K

T

A

N

R

E

D

N

L

I

R

L

I

V

G

G

A

E

Y

Y

T

S

Y

Y

I

Y

R

D

S

S

G

K

L

R

L

G

Q

E

S

S

V

F

S

E

E

D

-

Q

-

V

-

L

-
x
Y

-

H

-
x
Y

-

E

-

V

-

I

-

G

-

D

-

K

-

L

-

I

I

I

G

G

R

R

F

F

C

C

S

S

I

I

G
|
G

Q

P

E

G

V

T

Q

T

I

F

G

I

L

M

S

N

S
x
G

D

A

A
x
N

H
|
H

P

R

L
x
M

Y

D

W

G

L

-

T

S

T

T

H

Y

P

P

V

F

H
|
H

D
x
L

I

F

A

R

A

M

G

G

-

W

-

E

-

K

-

Y

-
x
M

-
x
P

-

S

L

L

K

K

Y

D

D

L

T

P

Q

L

K

K

A

G

H

D

A

I

R

E

I

I

G

G

H

N

D

D

V

V

W
|
W

I

I

G
|
G

A

R

G

D

V

V

K

T

I

I

L

M

S

P

G

G

V

V

T

K

V

I

G

G

D

D

G

G

A

A

V

I

I

I

A
|
A

T
x
A

G

E

A

A

V

V

T

T

R

K

H

N

V

V

A

A

P

P

Y

Y

E

S

I

I

V

V

G

G

N
|
N

P
|
P

A

L

K

K

H

F

I

I

K

R

W

K

R

R

F

F

E

S

D

D

S

G

I

V

L

I

R

-

D

E

E

E

I

W

H

L

K

A

S

L

A

Q

W

W

N

N

L

L

Q

D

F

M

E

K

Q

I

L

I

-

N

K

E

A

N

M

L

P

P

F

F

binding magnesium ion: N158 (= N162), P159 (= P163)
binding (3R,4R,5E,10E,12E,14S,16R,26aR)-16-fluoro-14-hydroxy-12-methyl-3-(propan-2-yl)-4-(prop-2-en-1-yl)-3,4,8,9,14,15,16,17,24,25,26,26a-dodecahydro-1H,7H,22H-21,18-(azeno)pyrrolo[2,1-c][1,8,4,19]dioxadiazacyclotetracosine-1,7,22-trione: Y53 (vs. gap), G78 (≠ S88), N80 (≠ A90), H81 (= H91), H91 (= H102), L92 (≠ D103), M101 (vs. gap), P102 (vs. gap)
binding thioacetic acid s-{2-[3-(2-hydroxy-3,3-dimethyl-4-phosphonooxy-butyrylamino)-propionylamino]-ethyl} ester: Y51 (vs. gap), G69 (= G79), H81 (= H91), M83 (≠ L93), W120 (= W124), G122 (= G126), A140 (= A144), A141 (≠ T145)

6pubA Crystal structure of the type b chloramphenicol acetyltransferase from vibrio cholerae in the complex with crystal violet
43% identity, 53% coverage: 72:194/234 of query aligns to 61:188/210 of 6pubA

query
sites
6pubA

I

I

G

G

R

S

F

F

C

C

S

S

I

I

G

G

Q

S

-

G

E

A

V

V

Q

F

I

M

G

M

L

A

S

G

S

N

D

Q

A

G

H

H

P

R

L

S

Y

D

W

W

L

I

T

S

T

T

H

F

P

P

V

F

H

F

-

Y

-

Q

-

D

-

N

-

D

D

N

I

F

A

A

A

D

G

A

L

R

K

D

Y

G

D

F

T

T

Q

R

K

S

A

G

H

D

A

T

R

I

I

I

G

G

H

H

D

D

V

V

W

W

I

I

G

G

A

T

G

E

V

A

K

M

I

I

L

M

S

P

G

G

V

V

T

K

V

I

G

G

D

H

G

G

A

A

V

I

I

I

A

A

T

S

G

R

A

S

V

V

T

T

R

K

H

D

V

V

A

A

P

P

Y

Y

E

E

I

V

V

V

G

G

G

S

N

N

P

P

A

A

K

K

H

H

I

I

K

K

W

F

R

R

F

F

E

S

D

D

S

V

I

E

L

I

R

A

D

-

E

M

I

L

H

L

K

E

S

M

A

A

W

W

N

N

L

W

Q

P

F

E

E

S

Q

W

L

L

K

K

Sites not aligning to the query:

binding crystal violet: 4, 7, 9, 10, 11, 36, 37

6u9cA The 2.2 a crystal structure of the type b chloramphenicol acetyltransferase from vibrio cholerae in the complex with acetyl coa
43% identity, 53% coverage: 72:194/234 of query aligns to 58:185/206 of 6u9cA

query
sites
6u9cA

I

I

G

G

R

S

F

F

C

C

S

S

I

I

G

G

Q

S

-

G

E

A

V

V

Q

F

I

M

G

M

L

A

S

G

S

N

D

Q

A

G

H

H

P

R

L

S

Y

D

W

W

L

I

T

S

T

T

H

F

P

P

V

F

H

F

-

Y

-

Q

-

D

-

N

-

D

D

N

I

F

A

A

A

D

G

A

L

R

K

D

Y

G

D

F

T

T

Q

R

K

S

A

G

H

D

A

T

R

I

I

I

G

G

H

H

D

D

V

V

W
|
W

I

I

G

G

A

T

G

E

V

A

K

M

I

I

L

M

S

P

G

G

V

V

T

K

V

I

G

G

D

H

G

G

A

A

V

I

I

I

A
|
A

T

S

G

R

A

S

V

V

T

T

R

K

H

D

V

V

A

A

P

P

Y

Y

E

E

I

V

V

V

G

G

G

S

N

N

P

P

A

A

K

K

H

H

I
|
I

K

K

W

F

R

R

F

F

E

S

D

D

S

V

I

E

L

I

R

A

D

-

E

M

I

L

H

L

K

E

S

M

A

A

W

W

N

N

L

W

Q

P

F

E

E

S

Q

W

L

L

K

K

binding acetyl coenzyme *a: W116 (= W124), A136 (= A144), I159 (= I167)

1khrA Crystal structure of vat(d) in complex with virginiamycin and coenzyme a (see paper)
34% identity, 59% coverage: 49:187/234 of query aligns to 26:183/206 of 1khrA

query
sites
1khrA

K

K

A

L

R

E

D

N

L

V

R

E

I

V

G

G

A

E

Y

Y

T

S

Y
|
Y

I

Y

R
x
D

S

S

-

K

-

N

-

G

-

E

-

T

-

F

-

D

-

K

-

Q

-

I

-

L

-

Y

-

H

-
x
Y

G

P

L

I

L

L

Q

N

S

D

V

K

S

L

E

K

I

I

G

G

R

K

F

F

C

C

S

S

I

I

G

G

Q

P

E

G

V

V

Q

T

I

I

G

I

L

M

S

N

S

G

D
x
A

A
x
N

H
|
H

P

R

L
x
M

Y

D

W

G

L

-

T

S

T

T

H

Y

P

P

V

F

H

N

D
x
L

I

F

A

G

A

N

G

G

-

W

-

E

-

K

-

H

-

M

-

P

-

K

L

L

K

D

Y

Q

D

L

T

P

Q

I

K

K

A

G

H

D

A

T

R

I

I

I

G

G

H

N

D

D

V

V

W
|
W

I

I

G

G

A

K

G

D

V

V

K

V

I

I

L

M

S

P

G

G

V

V

T

K

V

I

G

G

D

D

G

G

A

A

V

I

I

V

A
|
A

T
x
A

G

N

A

S

V

V

T
x
V

R

K

H

D

V

I

A

A

P

P

Y

Y

E

M

I
x
L

V

A

G
|
G

G

G

N

N

P
|
P

A

A

K

N

H

E

I
|
I

K

K

W

Q

R

R

F

F

E

D

D

Q

S

D

I

T

L

I

R

-

D

N

E

Q

I

L

H

L

K

D

S

I

A

K

W

W

N

N

binding coenzyme a: H82 (= H91), M84 (≠ L93), W121 (= W124), A141 (= A144), A142 (≠ T145), V147 (≠ T150), L155 (≠ I158), G157 (= G160), P160 (= P163), I164 (= I167)
binding virginiamycin m1: Y37 (= Y60), D39 (≠ R62), Y54 (vs. gap), A80 (≠ D89), N81 (≠ A90), H82 (= H91), L93 (≠ D103)

3dhoA Structure of streptogramin acetyltransferase in complex with an inhibitor
34% identity, 59% coverage: 49:187/234 of query aligns to 26:183/203 of 3dhoA

query
sites
3dhoA

K

K

A

L

R

E

D

N

L

V

R

E

I

V

G

G

A

E

Y

Y

T

S

Y

Y

I

Y

R

D

S

S

-

K

-

N

-

G

-

E

-

T

-

F

-

D

-

K

-

Q

-

I

-

L

-

Y

-

H

-

Y

G

P

L

I

L

L

Q

N

S

D

V

K

S

L

E

K

I

I

G

G

R

K

F

F

C

C

S

S

I

I

G

G

Q

P

E

G

V

V

Q

T

I

I

G

I

L

M

S

N

S

G

D

A

A

N

H

H

P

R

L
x
M

Y

D

W

G

L

-

T

S

T

T

H

Y

P

P

V

F

H

N

D

L

I

F

A

G

A

N

G

G

-

W

-

E

-

K

-

H

-

M

-

P

-

K

L

L

K

D

Y

Q

D

L

T

P

Q

I

K

K

A

G

H

D

A

T

R

I

I

I

G

G

H

N

D

D

V

V

W

W

I

I

G

G

A

K

G

D

V

V

K

V

I

I

L

M

S

P

G

G

V

V

T

K

V

I

G

G

D

D

G

G

A

A

V
x
I

I

V

A
|
A

T

A

G

N

A

S

V

V

T
x
V

R

K

H

D

V

I

A

A

P

P

Y

Y

E

M

I
x
L

V

A

G
|
G

G

G

N

N

P
|
P

A

A

K

N

H

E

I
|
I

K

K

W

Q

R

R

F

F

E

D

D

Q

S

D

I

T

L

I

R

-

D

N

E

Q

I

L

H

L

K

D

S

I

A

K

W

W

N

N

binding 6-bromo-N'-[(1Z)-(3,5-dichloro-2-hydroxyphenyl)methylidene]-2-methylquinoline-4-carbohydrazide: M84 (≠ L93), I139 (≠ V142), A141 (= A144), V147 (≠ T150), L155 (≠ I158), G157 (= G160), P160 (= P163), I164 (= I167)

1mrlA Crystal structure of streptogramin a acetyltransferase with dalfopristin (see paper)
34% identity, 59% coverage: 49:187/234 of query aligns to 26:183/204 of 1mrlA

query
sites
1mrlA

K

K

A

L

R

E

D

N

L

V

R

E

I

V

G

G

A

E

Y

Y

T

S

Y
|
Y

I

Y

R
x
D

S

S

-

K

-

N

-

G

-

E

-

T

-

F

-

D

-

K

-

Q

-

I

-

L

-

Y

-

H

-
x
Y

G

P

L
x
I

L

L

Q

N

S

D

V

K

S

L

E

K

I

I

G

G

R

K

F

F

C

C

S

S

I

I

G

G

Q

P

E

G

V

V

Q

T

I

I

G

I

L

M

S

N

S

G

D

A

A
x
N

H
|
H

P

R

L

M

Y

D

W

G

L

-

T

S

T

T

H

Y

P

P

V

F

H

N

D
x
L

I

F

A

G

A

N

G

G

-

W

-

E

-

K

-

H

-
x
M

-

P

-

K

L

L

K

D

Y

Q

D
x
L

T

P

Q

I

K

K

A

G

H

D

A

T

R

I

I

I

G

G

H

N

D

D

V

V

W

W

I

I

G

G

A

K

G

D

V

V

K

V

I

I

L

M

S

P

G

G

V

V

T

K

V

I

G

G

D

D

G

G

A

A

V

I

I

V

A

A

T

A

G

N

A

S

V

V

T

V

R

K

H

D

V

I

A

A

P

P

Y

Y

E

M

I

L

V

A

G

G

N

N

P

P

A

A

K

N

H

E

I

I

K

K

W

Q

R

R

F

F

E

D

D

Q

S

D

I

T

L

I

R

-

D

N

E

Q

I

L

H

L

K

D

S

I

A

K

W

W

N

N

binding 5-(2-diethylamino-ethanesulfonyl)-21-hydroxy-10-isopropyl-11,19-dimethyl-9,26-dioxa-3,15,28-triaza-tricyclo[23.2.1.00,255]octacosa-1(27),12,17,19,25(28)-pentaene-2,8,14,23-tetraone: Y37 (= Y60), D39 (≠ R62), Y54 (vs. gap), I56 (≠ L65), N81 (≠ A90), H82 (= H91), L93 (≠ D103), M102 (vs. gap), L108 (≠ D111)

1kk4A Crystal structure of vat(d) in complex with acetyl-coa (see paper)
34% identity, 59% coverage: 49:187/234 of query aligns to 26:183/205 of 1kk4A

query
sites
1kk4A

K

K

A

L

R

E

D

N

L

V

R

E

I

V

G

G

A

E

Y

Y

T

S

Y

Y

I

Y

R

D

S

S

-

K

-

N

-

G

-

E

-

T

-

F

-

D

-

K

-

Q

-

I

-

L

-

Y

-

H

-

Y

G

P

L

I

L

L

Q

N

S

D

V

K

S

L

E

K

I

I

G

G

R

K

F

F

C

C

S

S

I
|
I

G
|
G

Q

P

E

G

V

V

Q

T

I

I

G

I

L

M

S

N

S

G

D

A

A

N

H

H

P

R

L

M

Y

D

W

G

L

-

T

S

T

T

H

Y

P

P

V

F

H

N

D

L

I

F

A

G

A

N

G

G

-

W

-

E

-

K

-

H

-

M

-

P

-

K

L

L

K

D

Y

Q

D

L

T

P

Q

I

K
|
K

A

G

H

D

A

T

R

I

I

I

G

G

H

N

D

D

V

V

W
|
W

I

I

G
|
G

A
x
K

G

D

V

V

K

V

I

I

L

M

S

P

G

G

V

V

T

K

V

I

G

G

D

D

G

G

A

A

V

I

I

V

A
|
A

T
x
A

G

N

A

S

V

V

T
x
V

R
x
K

H

D

V

I

A

A

P

P

Y

Y

E

M

I
x
L

V

A

G
|
G

N

N

P
|
P

A

A

K

N

H

E

I
|
I

K

K

W

Q

R

R

F

F

E

D

D

Q

S

D

I

T

L

I

R

-

D

N

E

Q

I

L

H

L

K

D

S

I

A

K

W

W

N

N

binding acetyl coenzyme *a: I69 (= I78), G70 (= G79), K111 (= K114), W121 (= W124), G123 (= G126), K124 (≠ A127), A141 (= A144), A142 (≠ T145), V147 (≠ T150), K148 (≠ R151), L155 (≠ I158), G157 (= G160), G158 (= G161), P160 (= P163), I164 (= I167)

P50870 Streptogramin A acetyltransferase; Virginiamycin acetyltransferase D; Vat(D); EC 2.3.1.- from Enterococcus faecium (Streptococcus faecium) (see paper)
34% identity, 59% coverage: 49:187/234 of query aligns to 26:183/209 of P50870

query
sites
P50870

K

K

A

L

R

E

D

N

L

V

R

E

I

V

G

G

A

E

Y

Y

T

S

Y

Y

I

Y

R

D

S

S

-

K

-

N

-

G

-

E

-

T

-

F

-

D

-

K

-

Q

-

I

-

L

-

Y

-

H

-

Y

G

P

L

I

L

L

Q

N

S

D

V

K

S

L

E

K

I

I

G

G

R

K

F

F

C

C

S

S

I

I

G

G

Q

P

E

G

V

V

Q

T

I

I

G

I

L

M

S

N

S

G

D

A

A

N

H
|
H

P

R

L

M

Y

D

W

G

L

-

T

S

T

T

H

Y

P

P

V

F

H

N

D

L

I

F

A

G

A

N

G

G

-

W

-

E

-

K

-

H

-

M

-

P

-

K

L

L

K

D

Y

Q

D

L

T

P

Q

I

K

K

A

G

H

D

A

T

R

I

I

I

G

G

H

N

D

D

V

V

W

W

I

I

G

G

A

K

G

D

V

V

K

V

I

I

L

M

S

P

G

G

V

V

T

K

V

I

G

G

D

D

G

G

A

A

V

I

I

V

A

A

T

A

G

N

A

S

V

V

T

V

R

K

H

D

V

I

A

A

P

P

Y

Y

E

M

I

L

V

A

G

G

N

N

P

P

A

A

K

N

H

E

I

I

K

K

W

Q

R

R

F

F

E

D

D

Q

S

D

I

T

L

I

R

-

D

N

E

Q

I

L

H

L

K

D

S

I

A

K

W

W

N

N

H82 (= H91) mutation to A: 105-fold decrease in activity.

2xatA Complex of the hexapeptide xenobiotic acetyltransferase with chloramphenicol and desulfo-coenzyme a (see paper)
39% identity, 54% coverage: 72:198/234 of query aligns to 57:190/208 of 2xatA

query
sites
2xatA

I

I

G

G

R

S

F

F

C

C

S

S

I

I

G

G

Q

S

E

G

V

A

Q

A

I

F

G

I

L

M

S

A

S

G

D

N

-

Q

A

G

H

H

P

R

L

A

Y

E

W

W

L

A

T

S

T

T

H

F

P

P

V

F

H

H

D

F

I

M

-

H

-

E

-

P

-

A

A

F

A

A

G

G

L

A

K

V

Y

N

D

G

T

Y

Q

Q

K

P

A

A

-

G

H

D

A

T

R

L

I

I

G

G

H

H

D

E

V

V

W

W

I

I

G
|
G

A
x
T

G

E

V

A

K

M

I

F

L

M

S

P

G

G

V

V

T

R

V

V

G

G

D

H

G

G

A

A

V

I

I

I

A
x
G

T
x
S

G

R

A

A

V

L

V

V

T

T

R

G

H

D

V

V

A

E

P

P

Y

Y

E

A

I

I

V

V

G
|
G

N

N

P

P

A

A

K

R

H

T

I
|
I

K

R

W

K

R

R

F

F

E

S

D

D

S

G

I

D

L

I

R

Q

D

N

E

-

I

L

H

L

K

E

S

M

A

A

W

W

N

D

L

W

Q

P

F

L

E

A

Q

D

L

I

K

E

-

A

A

A

M

M

P

P

F

L

binding desulfo-coenzyme a: G118 (= G126), T119 (≠ A127), G136 (≠ A144), S137 (≠ T145), G152 (= G160), G153 (= G161), I159 (= I167)

Sites not aligning to the query:

binding chloramphenicol: 6, 7, 8, 28

6pxaE The crystal structure of chloramphenicol acetyltransferase-like protein from vibrio fischeri es114 in complex with taurocholic acid
35% identity, 53% coverage: 72:194/234 of query aligns to 73:197/221 of 6pxaE

query
sites
6pxaE

I

I

G

G

R

K

F

F

C

C

S

S

I

I

G

A

Q

S

E

G

V

A

Q

T

I

F

G

M

L

M

S

A

S

G

D

N

-
x
Q

A

G

H

H

P

R

L

A

Y

D

W

W

L

I

T

S

T

T

H
x
F

P
|
P

V
x
F

-
x
S

-

K

H

K

D

E

I
x
F

A

G

A

E

G

G

L

V

K

K

Y

D

D

G

T

F

Q

Q

K

R

A

A

H

G

A

D

R

T

I

I

-

V

G

G

H

N

D

D

V

V

W

W

I

I

G

G

A

S

G

E

V

A

K

M

I

I

L

M

S

P

G

G

V

V

T

H

V

I

G

G

D

D

G

G

A

A

V

I

I

I

A

G

T

A

G

R

A

A

V

V

V

I

T

T

R

K

H

N

V

V

A

A

P

P

Y

Y

E

S

I

V

V

V

G

V

G

G

N

N

P

N

A

V

K

V

H

V

I

K

K

K

W

-

R

R

F

F

E

D

D

E

S

N

I

L

L

I

R

Q

D

T

E

-

I

L

H

L

K

V

S

I

A

K

W

W

N

D

L

W

Q

P

F

L

E

Q

Q

H

L

I

K

K

binding taurocholic acid: Q91 (vs. gap), F101 (≠ H99), P102 (= P100), F103 (≠ V101), S104 (vs. gap), F108 (≠ I104)

Sites not aligning to the query:

binding taurocholic acid: 11, 12, 13, 32, 65

5ux9A The crystal structure of chloramphenicol acetyltransferase from vibrio fischeri es114
35% identity, 53% coverage: 72:194/234 of query aligns to 68:192/215 of 5ux9A

query
sites
5ux9A

I

I

G

G

R

K

F

F

C

C

S

S

I

I

G

A

Q

S

E

G

V

A

Q

T

I

F

G

M

L

M

S

A

S

G

D

N

-

Q

A

G

H

H

P

R

L

A

Y

D

W

W

L

I

T

S

T

T

H

F

P

P

V

F

-

S

-

K

H

K

D

E

I

F

A

G

A

E

G

G

L

V

K

K

Y

D

D

G

T

F

Q

Q

K

R

A

A

H

G

A

D

R

T

I

I

-

V

G

G

H

N

D

D

V

V

W

W

I

I

G

G

A

S

G

E

V

A

K

M

I

I

L

M

S

P

G

G

V

V

T

H

V

I

G

G

D

D

G

G

A

A

V

I

I

I

A

G

T

A

G

R

A

A

V

V

V

I

T

T

R

K

H

N

V

V

A

A

P

P

Y

Y

E

S

I

V

V

V

G

V

G

G

N

N

P

N

A

V

K

V

H

V

I

K

K

K

W

-

R

R

F

F

E

D

D

E

S

N

I

L

L

I

R

Q

D

T

E

-

I

L

H

L

K

V

S

I

A

K

W

W

N

D

L

W

Q

P

F

L

E

Q

Q

H

L

I

K

K

Sites not aligning to the query:

binding magnesium ion: 202, 205

3igjC Crystal structure of maltose o-acetyltransferase complexed with acetyl coenzyme a from bacillus anthracis
47% identity, 32% coverage: 95:168/234 of query aligns to 112:184/188 of 3igjC

query
sites
3igjC

W
x
Y

L

T

T
x
A

T

T

H
|
H

P

P

V

L

H

H

D

P

I

V

A

E

-

R

-

N

A

S

G

G

L

K

K

E

Y

Y

D

G

T

K

Q

P

K

-

A

-

H

-

A

V

R

K

I

I

G

G

H

N

D

N

V

V

W

W

I

V

G
|
G

A

G

G

G

V

A

K

I

I

I

L
x
N

S

P

G

G

V

V

T

S

V

I

G

G

D

D

G

N

A

A

V

V

I

I

A
|
A

T
x
S

G

G

A

A

V

V

T
|
T

R

K

H

D

V

V

A

P

P

N

Y

N

E

V

I

V

V

V

G

G

N
|
N

P

P

A

A

K

K

H

V

I

I

K

K

binding acetyl coenzyme *a: Y112 (≠ W95), A114 (≠ T97), H116 (= H99), G142 (= G126), N148 (≠ L132), A160 (= A144), S161 (≠ T145), T166 (= T150), N178 (= N162)

Sites not aligning to the query:

binding acetyl coenzyme *a: 84, 106

7uulA Crystal structure of aminoglycoside resistance enzyme apma, complex with kanamycin b and coenzyme a (see paper)
28% identity, 61% coverage: 52:194/234 of query aligns to 95:256/274 of 7uulA

query
sites
7uulA

D

N

L

V

R

P

I

V

G

G

A

R

Y

Q

T

T

Y
|
Y

I
x
F

R

G

S

D

G

G

L

V

Q

-

S

G

V

A

S

C

E

E

I
x
N

G

G

R

Y

F

I

C

K

S

S

I

I

G

G

Q

Q

E

F

V

T

Q

S

I

I

G
x
N

L

G

S

T

S

A

D

E

A

I

H

H

P

A

L
x
N

Y
x
H

W

Q

L
|
L

T

N

T

M

H

T

P

F

V

V

H

S

D
|
D

-
x
D

-

I

-

Q

-

N

-

F

-

N

-

E

-

S

-

M

-

A

-

V

I

F

A

Q

A

E

G

K

L

L

K

R

Y

K

D

D

T

P

Q

K

K

H

A

P

H

Y

A

A

-

Y

-

S

-

K

-

E

-

P

-

M

R

T

I

I

G

G

H

S

D

D

V

V

W
x
Y

I

I

G
|
G

A
|
A

G

H

V

A

K
x
F

I

I

L

N

S

A

G

S

V

T

-

V

-

T

T

S

V

I

G

G

D

D

G

G

A

A

V

I

I

I

A

G

T
x
S

G

G

A

A

V

V

T
x
L

R

E

H

N

V

V

A

P

P

P

Y

F

E

A

I

V

V

V

G
x
V

G

G

N

V

P

P

A

A

K

R

H

I

I
x
K

K
x
R

W

Y

R

R

F

F

E

S

D

K

S

E

I

M

L

I

R

-

D

E

E

T

I

L

H

L

K

R

S

V

A

K

W

W

N

D

L

W

Q

S

F

I

E

E

Q

E

L

I

K

N

binding (1r,2s,3s,4r,6s)-4,6-diamino-3-[(3-amino-3-deoxy-alpha-d-glucopyranosyl)oxy]-2-hydroxycyclohexyl 2,6-diamino-2,6-dideoxy-alpha-d-glucopyranoside: Y103 (= Y60), F104 (≠ I61), N114 (≠ I72), H136 (≠ Y94), D145 (= D103), D146 (vs. gap)
binding coenzyme a: N127 (≠ G85), N135 (≠ L93), L138 (= L96), Y185 (≠ W124), G187 (= G126), A188 (= A127), F191 (≠ K130), S208 (≠ T145), L213 (≠ T150), V223 (≠ G160), K230 (≠ I167), R231 (≠ K168)

Sites not aligning to the query:

binding (1r,2s,3s,4r,6s)-4,6-diamino-3-[(3-amino-3-deoxy-alpha-d-glucopyranosyl)oxy]-2-hydroxycyclohexyl 2,6-diamino-2,6-dideoxy-alpha-d-glucopyranoside: 82, 86

7uunA Crystal structure of aminoglycoside resistance enzyme apma, complex with neomycin (see paper)
28% identity, 61% coverage: 52:194/234 of query aligns to 95:256/273 of 7uunA

query
sites
7uunA

D

N

L

V

R

P

I

V

G

G

A

R

Y

Q

T

T

Y

Y

I

F

R

G

S

D

G

G

L

V

Q

-

S

G

V

A

S

C

E
|
E

I

N

G

G

R

Y

F

I

C

K

S

S

I

I

G

G

Q

Q

E

F

V

T

Q

S

I

I

G

N

L

G

S

T

S

A

D

E

A

I

H

H

P

A

L

N

Y
x
H

W

Q

L

L

T

N

T

M

H

T

P

F

V

V

H

S

D
|
D

-
x
D

-

I

-

Q

-

N

-

F

-

N

-

E

-

S

-

M

-

A

-

V

I

F

A

Q

A

E

G

K

L

L

K

R

Y

K

D

D

T

P

Q

K

K

H

A

P

H

Y

A

A

-

Y

-

S

-

K

-

E

-

P

-

M

R

T

I

I

G

G

H

S

D

D

V

V

W

Y

I

I

G

G

A

A

G

H

V

A

K

F

I

I

L

N

S

A

G

S

V

T

-

V

-

T

T

S

V

I

G

G

D

D

G

G

A

A

V

I

I

I

A

G

T

S

G

G

A

A

V

V

T

L

R

E

H

N

V

V

A

P

P

P

Y

F

E

A

I

V

V

V

G

V

G

G

N

V

P

P

A

A

K

R

H

I

I

K

K

R

W

Y

R

R

F

F

E

S

D

K

S

E

I

M

L

I

R

-

D

E

E

T

I

L

H

L

K

R

S

V

A

K

W

W

N

D

L

W

Q

S

F

I

E

E

Q

E

L

I

K

N

binding neomycin: E113 (= E71), H136 (≠ Y94), D145 (= D103), D146 (vs. gap)

7uumA Crystal structure of aminoglycoside resistance enzyme apma, complex with paromomycin and coenzyme a (see paper)
28% identity, 61% coverage: 52:194/234 of query aligns to 96:257/274 of 7uumA

query
sites
7uumA

D

N

L

V

R

P

I

V

G

G

A

R

Y

Q

T

T

Y

Y

I

F

R

G

S

D

G

G

L

V

Q

-

S

G

V

A

S

C

E
|
E

I
x
N

G

G

R

Y

F

I

C

K

S

S

I

I

G

G

Q

Q

E

F

V

T

Q

S

I

I

G

N

L

G

S

T

S

A

D

E

A

I

H
|
H

P

A

L
x
N

Y
x
H

W

Q

L

L

T

N

T

M

H

T

P

F

V

V

H

S

D
|
D

-
x
D

-

I

-

Q

-

N

-

F

-

N

-

E

-

S

-

M

-

A

-

V

I

F

A

Q

A

E

G

K

L

L

K

R

Y

K

D

D

T

P

Q
x
K

K

H

A

P

H

Y

A

A

-

Y

-

S

-

K

-

E

-

P

-

M

R

T

I

I

G

G

H

S

D

D

V

V

W

Y

I

I

G

G

A

A

G

H

V

A

K
x
F

I

I

L

N

S

A

G

S

V

T

-

V

-

T

T

S

V

I

G

G

D

D

G

G

A

A

V

I

I

I

A

G

T

S

G

G

A

A

V

V

T

L

R

E

H

N

V

V

A

P

P

P

Y

F

E

A

I

V

V

V

G

V

G

G

N

V

P
|
P

A

A

K

R

H

I

I

K

K

R

W

Y

R

R

F

F

E

S

D

K

S

E

I

M

L

I

R

-

D

E

E

T

I

L

H

L

K

R

S

V

A

K

W

W

N

D

L

W

Q

S

F

I

E

E

Q

E

L

I

K

N

binding coenzyme a: H134 (= H91), N136 (≠ L93), K169 (≠ Q113), F192 (≠ K130), P227 (= P163)
binding paromomycin: E114 (= E71), N115 (≠ I72), H137 (≠ Y94), D146 (= D103), D147 (vs. gap)

7uukC Crystal structure of aminoglycoside resistance enzyme apma, complex with tobramycin (see paper)
28% identity, 61% coverage: 52:194/234 of query aligns to 96:257/276 of 7uukC

query
sites
7uukC

D

N

L

V

R

P

I

V

G

G

A

R

Y

Q

T

T

Y
|
Y

I
x
F

R

G

S

D

G

G

L

V

Q

-

S

G

V

A

S

C

E

E

I
x
N

G

G

R

Y

F

I

C

K

S

S

I

I

G

G

Q

Q

E

F

V

T

Q

S

I

I

G

N

L

G

S

T

S

A

D

E

A

I

H

H

P

A

L

N

Y
x
H

W

Q

L

L

T

N

T

M

H

T

P

F

V

V

H

S

D
|
D

-
x
D

-

I

-

Q

-

N

-

F

-

N

-

E

-

S

-

M

-

A

-

V

I

F

A

Q

A

E

G

K

L

L

K

R

Y

K

D

D

T

P

Q

K

K

H

A

P

H

Y

A

A

-

Y

-

S

-

K

-

E

-

P

-

M

R

T

I

I

G

G

H

S

D

D

V

V

W

Y

I

I

G

G

A

A

G

H

V

A

K

F

I

I

L

N

S

A

G

S

V

T

-

V

-

T

T

S

V

I

G

G

D

D

G

G

A

A

V

I

I

I

A

G

T

S

G

G

A

A

V

V

T

L

R

E

H

N

V

V

A

P

P

P

Y

F

E

A

I

V

V

V

G

V

G

G

N

V

P

P

A

A

K

R

H

I

I

K

K

R

W

Y

R

R

F

F

E

S

D

K

S

E

I

M

L

I

R

-

D

E

E

T

I

L

H

L

K

R

S

V

A

K

W

W

N

D

L

W

Q

S

F

I

E

E

Q

E

L

I

K

N

binding tobramycin: Y104 (= Y60), F105 (≠ I61), N115 (≠ I72), H137 (≠ Y94), D146 (= D103), D147 (vs. gap)

Sites not aligning to the query:

binding tobramycin: 83, 87

Query Sequence

>GFF460 FitnessBrowser__psRCH2:GFF460
MVIDFVRSYYWKRWLRRRDVKLAAGLASLTASAQVVVEENVRLGDVAIKARDLRIGAYTY
IRSGLLQSVSEIGRFCSIGQEVQIGLSSDAHPLYWLTTHPVHDIAAGLKYDTQKAHARIG
HDVWIGAGVKILSGVTVGDGAVIATGAVVTRHVAPYEIVGGNPAKHIKWRFEDSILRDEI
HKSAWWNLQFEQLKAMPFDNPPECLRYISALGGKAGGGASYSSYRIRKSGCAAL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory