SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF4691 FitnessBrowser__WCS417:GFF4691 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4mvaA 1.43 angstrom resolution crystal structure of triosephosphate isomerase (tpia) from escherichia coli in complex with acetyl phosphate. (see paper)
50% identity, 99% coverage: 1:249/251 of query aligns to 1:249/255 of 4mvaA

query
sites
4mvaA

M

M

R

R

R

H

T

P

M

L

V

V

A

M

G

G

N

N

W

W

K

K

M

L

H

N

G

G

T

S

R

R

A

H

S

M

V

V

A

H

E

E

L

L

I

V

N

S

G

N

L

L

R

R

-

K

H

E

L

L

A

A

L

G

P

V

S

A

G

G

V

C

D

A

V

V

A

A

V

I

F

A

P

P

C

E

L

M

H

Y

I

I

N

D

Q

M

V

A

V

K

D

R

G

E

L

A

K

E

G

G

K

S

S

H

I

I

Q

M

V

L

G

G

A

A

Q

Q

N

N

S

V

A

D

V

L

E

N

P

-

M

L

Q

S

G

G

A

A

L

F

T

T

G

G

E

E

I

T

S

S

P

A

S

A

Q

M

L

L

V

K

D

D

A

I

G

G

C

A

S

Q

Y

Y

V

I

L

I

V

I

G

G

H

H

S

S

E

E

R

R

Q

T

M

Y

M

H

G

K

E

E

R

S

D

D

G

E

T

L

L

I

N

A

R

K

K

K

F

F

A

A

A

V

A

L

Q

K

A

E

C

Q

G

G

L

L

I

T

P

P

V

V

L

L

C

C

I

I

G

G

E

E

T

T

L

E

E

A

Q

E

R

N

E

E

S

A

G

G

K

K

T

T

L

E

E

E

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V

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C

S

A

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R

Q

Q

L

I

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D

S

A

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I

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E

K

E

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L

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G

G

V

A

G

A

A

A

F

F

A

E

K

G

A

A

V

V

I

I

A

A

Y

Y

E

E

P

P

V

V

W

W

A

A

I
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I

G

G

T

T

G

G

L

K

T

S

A

A

T

T

P

P

Q

A

Q

Q

A

A

Q

Q

D

A

V

V

H

H

A

K

A

F

I

I

R

R

A

D

Q

H

L

I

A

A

A

K

E

V

N

D

S

A

E

N

V

I

A

A

Q

E

G

Q

V

V

R

I

L

I

L

Q

Y

Y

G

G

S
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S

V

V

K

N

A

A

A

S

N

N

A

A

V

A

E

E

L

L

F

F

G

A

M

Q

P

P

D

D

I

I

D

D

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G

G

A

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G

A

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S

S

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L

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K

A

A

D

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F

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A

A

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I

I

C

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R

K

A

A

binding acetylphosphate: I172 (= I172), S212 (= S212), G233 (= G233), G234 (= G234)

B1XB85 Triosephosphate isomerase; TIM; TPI; Triose-phosphate isomerase; EC 5.3.1.1 from Escherichia coli (strain K12 / DH10B) (see paper)
50% identity, 99% coverage: 1:249/251 of query aligns to 1:249/255 of B1XB85

query
sites
B1XB85

M

M

R

R

R

H

T

P

M

L

V

V

A

M

G

G

N

N

W

W

K

K

M

L

H

N

G

G

T

S

R

R

A

H

S

M

V

V

A

H

E

E

L

L

I

V

N

S

G

N

L

L

R

R

-

K

H

E

L

L

A

A

L

G

P

V

S

A

G

G

V

C

D

A

V

V

A

A

V

I

F

A

P

P

C

E

L

M

H

Y

I

I

N

D

Q

M

V

A

V

K

D

R

G

E

L

A

K

E

G

G

K

S

S

H

I

I

Q

M

V

L

G

G

A

A

Q

Q

N

N

S

V

A

D

V

L

E

N

P

-

M

L

Q

S

G

G

A

A

L

F

T

T

G

G

E

E

I

T

S

S

P

A

S

A

Q

M

L

L

V

K

D

D

A

I

G

G

C

A

S

Q

Y

Y

V

I

L

I

V

I

G

G

H

H

S

S

E

E

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R

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T

M

Y

M

H

G

K

E

E

R

S

D

D

G

E

T

L

L

I

N

A

R

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K

K

F

F

A

A

A

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K

A

E

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Q

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G

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P

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V

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L

C

C

I

I

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G

E

E

T

T

L

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E

A

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N

E

E

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A

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G

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K

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T

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E

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A

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S

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G

A

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F

F

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G

A

A

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V

I

I

A

A

Y

Y

E

E

P

P

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V

W

W

A

A

I

I

G

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T

G

G

L

K

T

S

A

A

T

T

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P

Q

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Q

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A

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A

V

V

H

H

A

K

A

F

I

I

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R

A

D

Q

H

L

I

A

A

A

K

E

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N

D

S

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E

N

V

I

A

A

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E

G

Q

V

V

R

I

L

I

L

Q

Y

Y

G

G

S
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S

V

V

K

N

A

A

A

S

N

N

A

A

V

A

E

E

L

L

F

F

G

A

M

Q

P

P

D

D

I

I

D

D

G

G

G

A

L

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I

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G
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G

A

A

S

S

L

L

N

K

A

A

D

D

E

A

F

F

G

A

A

V

I

I

C

V

R

K

A

A

S212 (= S212) binding
GG 233:234 (= GG 233:234) binding

6neeB Crystal structure of a reconstructed ancestor of triosephosphate isomerase from eukaryotes (see paper)
50% identity, 98% coverage: 2:248/251 of query aligns to 4:250/252 of 6neeB

query
sites
6neeB

R

R

R

K

T

F

M

F

V

V

A

G

G

G

N
|
N

W

W

K
|
K

M

M

H

N

G

G

T

T

R

L

A

E

S

S

V

I

A

K

E

A

L

L

I

V

N

E

G

T

L

L

R

N

H

S

L

A

A

Q

L

L

P

D

S

P

G

N

V

T

D

E

V

V

A

V

V

V

F

A

P

P

C

A

L

I

H

Y

I

L

N

P

Q

F

V

A

V

R

D

S

G

K

L

L

K

K

G

K

-

P

K

K

S

E

I

I

Q

Q

V

V

G

A

A

A

Q

Q

N

N

S

C

A

Y

V

T

E

K

P

P

M

-

Q

N

G

G

A

A

L

F

T

T

G

G

E

E

I

I

S

S

P

A

S

E

Q

M

L

L

V

K

D

D

A

L

G

G

C

V

S

P

Y

W

V

V

L

I

V

L

G

G

H
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H

S

S

E

E

R

R

Q

T

M

I

M

F

G

G

E

E

R

S

D

D

G

E

T

L

L

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N

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E

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K

F

V

A

K

A

Y

A

A

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L

A

D

C

Q

G

G

L

L

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V

I

L

A

C

C

I

I

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G

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E

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L

L

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Q

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R

R

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A

G

G

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K

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M

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A

I

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D

L

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G

K

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I

G

K

A

D

F

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A

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K

N

A

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V

V

I

I

A

A

Y

Y

E
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E

P

P

V

V

W

W

A

A

I
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I

G
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G

T

T

G

G

L

K

T

V

A

A

T

T

P

P

Q

E

Q

Q

A

A

Q

Q

D

E

V

V

H

H

A

A

I

L

R

R

A

K

Q

W

L

L

A

K

A

E

E

N

N

V

S

S

-

P

E

E

V

V

A

A

Q

E

G

S

V

T

R

R

L

I

Y

Y

G

G

G
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G

S
|
S

V

V

K

N

A

A

N

N

A

C

V

K

E

E

L

L

F

A

G

K

M

Q

P

P

D

D

I

I

D

D

G

G

G

F

L
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L

I
x
V

G
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G

A

A

S

S

L

L

N

K

A

A

D

P

E

E

F

F

G

V

A

D

I

I

C

I

R

N

A

A

binding phosphoglycolohydroxamic acid: N11 (= N9), K13 (= K11), H97 (= H95), E168 (= E167), I173 (= I172), G174 (= G173), G213 (= G211), S214 (= S212), L233 (= L231), V234 (≠ I232), G235 (= G233), G236 (= G234)

P50921 Triosephosphate isomerase; TIM; TPI; Triose-phosphate isomerase; EC 5.3.1.1 from Moritella marina (Vibrio marinus) (see paper)
47% identity, 95% coverage: 1:238/251 of query aligns to 1:240/256 of P50921

query
sites
P50921

M

M

R

R

R

H

T

P

M

V

V

V

A

M

G

G

N
|
N

W
|
W

K
|
K

M

L

H

N

G

G

T

S

R

K

A

E

S

M

V

V

A

V

E

D

L

L

I

L

N

N

G

G

L

L

R

N

-

A

H

E

L

L

A

E

L

G

P

V

S

T

G

G

V

V

D

D

V

V

A

A

V

V

F

A

P

P

C

A

L

L

H

F

I

V

N

D

Q

L

V

A

V

E

D

R

G

T

L

L

K

T

-

E

-

A

G

G

K

S

S

A

I

I

Q

I

V

L

G

G

A

A

Q

Q

N

N

S

T

A

D

V

L

E

N

P

N

M

-

Q

S

G

G

A

A

L

F

T

T

G

G

E

D

I

M

S

S

P

P

S

A

Q

M

L

L

V

K

D

E

A

F

G

G

C

A

S

T

Y

H

V

I

L

I

V

I

G

G

H

H

S

S

E

E

R

R

Q

E

M

Y

M

H

G

A

E

E

R

S

D

D

G

E

T

F

L

V

N

A

R

K

K

K

F

F

A

A

A

F

A

L

Q

K

A

E

C

N

G

G

L

L

I

T

P

P

V

V

L

L

C

C

I

I

G

G

E

E

T

S

L

D

E

A

Q

Q

R

N

E

E

S

A

G

G

K

E

T

T

L

M

E

A

V

V

V

C

S

A

R

R

Q

Q

L

L

G

D

S

A

I

V

I

I

E

N

E

T

L

Q

G

G

V

V

G

E

A

A

F

L

A

E

K

G

A

A

V

I

I

I

A

A

Y

Y

E

E

P

P

V

I

W

W

A

A

I

I

G
|
G

T

T

G

G

L

K

T

A

A

A

T

T

P

A

Q

E

Q

D

A

A

Q

Q

D

R

V

I

H

H

A

A

A

Q

I

I

R

R

A

A

Q

H

L

I

A

A

A

E

E

K

N

S

S

E

E

A

V

V

A

A

Q

K

G

N

V

V

R

V

L

I

L

Q

Y

Y

G

G

S
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S

V

V

K

K

A

P

A

E

N

N

A

A

V

A

E

A

L

Y

F

F

G

A

M

Q

P

P

D

D

I

I

D

D

G

G

G

A

L

L

I

V

G
|
G

A

A

S
x
A

L

L

N

D

NWK 9:11 (= NWK 9:11) binding
G175 (= G173) binding
S214 (= S212) binding
GG 235:236 (= GG 233:234) binding
A238 (≠ S236) mutation to S: Reduces catalytic efficiency and affinity, but increases thermal stability.

1aw2A Triosephosphate isomerase of vibrio marinus (see paper)
47% identity, 94% coverage: 2:238/251 of query aligns to 1:239/255 of 1aw2A

query
sites
1aw2A

R

R

R

H

T

P

M

V

V

V

A

M

G

G

N
|
N

W

W

K
|
K

M

L

H

N

G

G

T

S

R

K

A

E

S

M

V

V

A

V

E

D

L

L

I

L

N

N

G

G

L

L

R

N

-

A

H

E

L

L

A

E

L

G

P

V

S

T

G

G

V

V

D

D

V

V

A

A

V

V

F

A

P

P

C

A

L

L

H

F

I

V

N

D

Q

L

V

A

V

E

D

R

G

T

L

L

K

T

-

E

-

A

G

G

K

S

S

A

I

I

Q

I

V

L

G

G

A

A

Q

Q

N

N

S

T

A

D

V

L

E

N

P

N

M

-

Q

S

G

G

A

A

L

F

T

T

G

G

E

D

I

M

S

S

P

P

S

A

Q

M

L

L

V

K

D

E

A

F

G

G

C

A

S

T

Y

H

V

I

L

I

V

I

G

G

H
|
H

S

S

E
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E

R

R

Q

E

M

Y

M

H

G

A

E

E

R

S

D

D

G

E

T

F

L

V

N

A

R

K

K

K

F

F

A

A

A

F

A

L

Q

K

A

E

C

N

G

G

L

L

I

T

P

P

V

V

L

L

C

C

I

I

G

G

E

E

T

S

L

D

E

A

Q

Q

R

N

E

E

S

A

G

G

K

E

T

T

L

M

E

A

V

V

V

C

S

A

R

R

Q

Q

L

L

G

D

S

A

I

V

I

I

E

N

E

T

L

Q

G

G

V

V

G

E

A

A

F

L

A

E

K

G

A

A

V

I

I

I

A

A

Y

Y

E
|
E

P

P

V

I

W

W

A

A

I

I

G
|
G

T

T

G

G

L

K

T

A

A

A

T

T

P

A

Q

E

Q

D

A

A

Q

Q

D

R

V

I

H

H

A

A

A

Q

I

I

R

R

A

A

Q

H

L

I

A

A

A

E

E

K

N

S

S

E

E

A

V

V

A

A

Q

K

G

N

V

V

R

V

L

I

L

Q

Y

Y

G

G

S
|
S

V
|
V

K

K

A

P

A

E

N

N

A

A

V

A

E

A

L

Y

F

F

G

A

M

Q

P

P

D

D

I

I

D

D

G

G

G

A

L

L

I

V

G
|
G

A

A

S

A

L

L

N

D

active site: N8 (= N9), K10 (= K11), H96 (= H95), E98 (= E97), E168 (= E167), G174 (= G173), S213 (= S212)
binding sulfate ion: S213 (= S212), V214 (= V213), G234 (= G233), G235 (= G234)

1aw1A Triosephosphate isomerase of vibrio marinus complexed with 2-phosphoglycolate (see paper)
47% identity, 94% coverage: 2:238/251 of query aligns to 1:239/255 of 1aw1A

query
sites
1aw1A

R

R

R

H

T

P

M

V

V

V

A

M

G

G

N
|
N

W

W

K
|
K

M

L

H

N

G

G

T

S

R

K

A

E

S

M

V

V

A

V

E

D

L

L

I

L

N

N

G

G

L

L

R

N

-

A

H

E

L

L

A

E

L

G

P

V

S

T

G

G

V

V

D

D

V

V

A

A

V

V

F

A

P

P

C

A

L

L

H

F

I

V

N

D

Q

L

V

A

V

E

D

R

G

T

L

L

K

T

-

E

-

A

G

G

K

S

S

A

I

I

Q

I

V

L

G

G

A

A

Q

Q

N

N

S

T

A

D

V

L

E

N

P

N

M

-

Q

S

G

G

A

A

L

F

T

T

G

G

E

D

I

M

S

S

P

P

S

A

Q

M

L

L

V

K

D

E

A

F

G

G

C

A

S

T

Y

H

V

I

L

I

V

I

G

G

H
|
H

S

S

E
|
E

R

R

Q

E

M

Y

M

H

G

A

E

E

R

S

D

D

G

E

T

F

L

V

N

A

R

K

K

K

F

F

A

A

A

F

A

L

Q

K

A

E

C

N

G

G

L

L

I

T

P

P

V

V

L

L

C

C

I

I

G

G

E

E

T

S

L

D

E

A

Q

Q

R

N

E

E

S

A

G

G

K

E

T

T

L

M

E

A

V

V

V

C

S

A

R

R

Q

Q

L

L

G

D

S

A

I

V

I

I

E

N

E

T

L

Q

G

G

V

V

G

E

A

A

F

L

A

E

K

G

A

A

V

I

I

I

A

A

Y

Y

E
|
E

P

P

V

I

W

W

A

A

I
|
I

G
|
G

T

T

G

G

L

K

T

A

A

A

T

T

P

A

Q

E

Q

D

A

A

Q

Q

D

R

V

I

H

H

A

A

A

Q

I

I

R

R

A

A

Q

H

L

I

A

A

A

E

E

K

N

S

S

E

E

A

V

V

A

A

Q

K

G

N

V

V

R

V

L

I

L

Q

Y

Y

G
|
G

S
|
S

V

V

K

K

A

P

A

E

N

N

A

A

V

A

E

A

L

Y

F

F

G

A

M

Q

P

P

D

D

I

I

D

D

G

G

G

A

L
|
L

I

V

G
|
G

A

A

S

A

L

L

N

D

active site: N8 (= N9), K10 (= K11), H96 (= H95), E98 (= E97), E168 (= E167), G174 (= G173), S213 (= S212)
binding 2-phosphoglycolic acid: K10 (= K11), H96 (= H95), E168 (= E167), I173 (= I172), G174 (= G173), G211 (= G210), G212 (= G211), S213 (= S212), L232 (= L231), G234 (= G233), G235 (= G234)

P36204 Bifunctional PGK/TIM; EC 2.7.2.3; EC 5.3.1.1 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
48% identity, 94% coverage: 2:238/251 of query aligns to 402:639/654 of P36204

query
sites
P36204

R

R

R

K

T

L

M

I

V

L

A

A

G

G

N

N

W

W

K

K

M

M

H

H

G

K

T

T

-

I

-

S

-

E

-

A

R

K

A

K

S

F

V

V

A

S

E

L

L

L

I

V

N

N

G

E

L

L

R

H

H

D

L

V

A

-

L

-

P

-

S

K

G

E

V

F

D

E

V

I

A

V

V

V

F

C

P

P

C

F

L

T

H

A

I

L

N

S

Q

E

V

V

V

G

D

E

G

I

L

L

K

S

G

G

K

R

S

N

I

I

Q

K

V

L

G

G

A

A

Q

Q

N

N

S

V

A

F

V

Y

E

E

P

D

M

-

Q

Q

G

G

A

A

L

F

T

T

G

G

E

E

I

I

S

S

P

P

S

L

Q

M

L

L

V

Q

D

E

A

I

G

G

C

V

S

E

Y

Y

V

V

L

I

V

V

G

G

H

H

S

S

E

E

R

R

Q

R

M

I

M

F

G

K

E

E

R

D

D

D

G

E

T

F

L

I

N

N

R

R

K

K

F

V

A

K

A

A

A

V

Q

L

A

E

C

K

G

G

L

M

I

T

P

P

V

I

L

L

C

C

I

V

G

G

E

E

T

T

L

L

E

E

Q

E

R

R

E

E

S

K

G

G

K

L

T

T

L

F

E

C

V

V

S

E

R

K

Q

Q

L

V

G

R

S

E

I

G

I

F

E

Y

E

G

L

L

G

D

V

K

G

E

A

E

F

A

A

K

K

R

A

V

V

V

I

I

A

A

Y

Y

E

E

P

P

V

V

W

W

A

A

I

I

G

G

T

T

G

G

L

R

T

V

A

A

T

T

P

P

Q

Q

A

A

Q

Q

D

E

V

V

H

H

A

A

A

F

I

I

R

R

A

K

Q

L

L

L

A

S

A

E

-

M

E

Y

N

D

S

E

E

E

V

T

A

A

Q

G

G

S

V

I

R

R

L

I

L

L

Y

Y

G

G

S

S

V

I

K

K

A

P

A

D

N

N

A

F

V

L

E

G

L

L

F

I

G

V

M

Q

P

K

D

D

I

I

D

D

G

G

L

L

I

V

G

G

A

A

S

S

L

L

N

K

Sites not aligning to the query:

36 binding
118 binding
151 binding

4y96A Crystal structure of triosephosphate isomerase from gemmata obscuriglobus (see paper)
46% identity, 99% coverage: 2:250/251 of query aligns to 2:250/250 of 4y96A

query
sites
4y96A

R

R

R

K

T

K

M

F

V

V

A

A

G

G

N
|
N

W

W

K
|
K

M

M

H

N

G

T

T

T

R

L

A

A

S

E

V

A

A

K

E

A

L

L

I

G

N

A

G

A

L

V

R

A

H

K

L

G

A

V

L

T

P

D

S

D

G

R

V

V

D

T

V

V

A

A

V

V

F

F

P

P

C

Y

L

P

H

W

I

L

N

T

Q

A

V

V

V

G

D

E

G

V

L

L

K

K

G

G

K

S

S

P

I

V

Q

A

V

L

G

G

A

A

Q

Q

N

D

S

V

A

S

V

S

E

E

P

K

M

-

Q

K

G

G

A

A

L

F

T

T

G

G

E

E

I

V

S

S

P

P

S

A

Q

M

L

L

V

L

D

E

A

T

G

G

C

C

S

K

Y

Y

V

A

L

L

V

I

G

G

H
|
H

S

S

E
|
E

R

R

Q

H

M

I

G

G

E

E

R

S

D

E

G

T

T

F

L

I

N

N

R

H

K

K

F

V

A

H

A

T

A

A

Q

L

A

E

C

E

G

G

L

L

I

S

P

V

V

V

L

L

C

C

I

M

G

G

E

E

T

T

L

L

E

A

Q

E

R

R

E

E

S

R

G

G

K

L

T

Q

L

E

E

R

V

V

V

F

S

Q

R

R

Q

Q

L

V

G

Y

S

A

I

A

I

C

E

A

E

G

L

L

G

T

V

D

G

E

A

Q

F

F

A

G

K

R

A

I

V

V

I

I

A

A

Y

Y

E
|
E

P

P

V

V

W

W

A

A

I
|
I

G
|
G

T

T

G

G

L

K

T

V

A

A

T

T

P

P

Q

E

Q

Q

A

A

Q

Q

D

E

V

A

H

H

A

A

A

F

I

V

R

R

A

S

Q

K

L

L

A

R

-

L

A

L

E

Y

N
x
G

S

D

E
x
K

V

I

A

A

Q

D

G

S

V

T

R

P

L

I

L

V

Y

Y

G

G

S
|
S

V

V

K

T

A

P

A

D

N

N

A

T

V

V

E

G

L

L

F

M

G

S

M

Q

P

P

D

D

I

V

D

D

G

G

G

A

L

L

I

V

G
|
G

A

A

S

S

L

L

N

K

A

A

D

D

E

S

F

F

G

L

A

A

I

I

C

V

R

K

A

A

G

G

active site: N9 (= N9), K11 (= K11), H94 (= H95), E96 (= E97), E166 (= E167), G172 (= G173), S212 (= S212)
binding calcium ion: G198 (≠ N198), K200 (≠ E200)
binding phosphate ion: I171 (= I172), G172 (= G173), S212 (= S212), G233 (= G233), G234 (= G234)

P00942 Triosephosphate isomerase; TIM; Triose-phosphate isomerase; EC 5.3.1.1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 2 papers)
46% identity, 94% coverage: 2:237/251 of query aligns to 3:236/248 of P00942

query
sites
P00942

R

R

R

T

T

F

M

F

V

V

A

G

G

G

N
|
N

W

F

K
|
K

M

L

H

N

G

G

T

S

R

K

A

Q

S

S

V

I

A

K

E

E

L

I

I

V

N

E

G

R

L

L

R

N

H

T

L

A

A

S

L

I

P

P

S

E

G

N

V

V

D

E

V

V

A

V

V

I

F

C

P

P

C

A

L

T

H

Y

I

L

N

D

Q

Y

V

S

V

V

D

S

G

L

L

V

K

K

G

K

K

P

S

Q

I

V

Q

T

V

V

G

G

A

A

Q

Q

N

N

S

A

A

Y

V

L

E

K

P

A

M

-

Q

S

G

G

A

A

L

F

T

T

G

G

E

E

I

N

S

S

P

V

S

D

Q

Q

L

I

V

K

D

D

A

V

G

G

C

A

S

K

Y

W

V

V

L

I

V

L

G

G

H

H

S

S

E

E

R

R

Q

S

M

Y

M

F

G

H

E

E

R

D

D

D

G

K

T

F

L

I

-

A

-

D

N

K

R

T

K

K

F

F

A

A

A

L

A

G

Q

Q

A

G

C

V

G

G

L

V

I

I

P

-

V

-

L

L

C

C

I

I

G

G

E

E

T

T

L

L

E

E

Q

E

R

K

E

K

S

A

G

G

K

K

T

T

L

L

E

D

V

V

S

E

R

R

Q

Q

L

L

G

N

S

A

I

V

I

L

E

E

L

-

G

-

V

V

G

K

A

D

F

W

A

T

K

N

A

V

V

V

I

V

A

A

Y

Y

E

E

P

P

V

V

W

W

A

A

I

I

G

G

T

T

G

G

L

L

T

A

A

A

T

T

P

P

Q

E

Q

D

A

A

Q

Q

D

D

V

I

H

H

A

A

A

S

I

I

R

R

A

K

Q

F

L

L

A

A

A

S

E

K

-

L

N

G

S

D

E

K

V

A

A

A

Q

S

G

E

V

L

R

R

L

I

L

L

Y

Y

G

G

S

S

V

A

K

N

A

G

A

S

N

N

A

A

V

V

E

T

L

F

F

K

G

D

M

K

P

A

D

D

I

V

D

D

G

G

G

F

L

L

I

V

G

G

A

A

S

S

L

L

N10 (= N9) binding
K12 (= K11) binding

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

5zfxB Crystal structure of triosephosphate isomerase from opisthorchis viverrini (see paper)
47% identity, 98% coverage: 2:248/251 of query aligns to 1:246/248 of 5zfxB

query
sites
5zfxB

R

R

R

K

T

F

M

F

V

V

A

G

G

G

N
|
N

W

W

K
|
K

M

M

H

N

G

G

T

S

R

K

A

K

S

E

V

N

A

D

E

K

L

L

I

I

N
x
E

G

M

L

L

R

T

H
|
H

L

A

A

K

L

I

P

D

S

P

G

N

V

T

D

E

V

V

A

L

V

V

F

A

P

P

C

A

L

L

H

Y

I

L

N

P

Q

S

V

V

V

R

D

E

G

K

L

L

K

-

G

D

K

K

S

R

I

F

Q

H

V

V

G

A

A

A

Q

Q

N

N

S

C

A

Y

V

K

E

V

P

P

M

-

Q

S

G

G

A

A

L

F

T

T

G

G

E

E

I

V

S

S

P

P

S

A

Q

M

L

L

V

K

D

D

A

V

G

G

C

C

S

D

Y

W

V

V

L

I

V

L

G

G

H
|
H

S

S

E
|
E

R

R

Q

H

M

I

M

L

G

L

E

E

R

T

D

D

G

Q

T

L

L

V

N

G

R

E

K

K

F

T

A

N

A

H

A

A

Q

I

A

S

C

A

G

G

L

V

I

N

P

V

V

I

L

A

C

C

I

I

G

G

E

E

T

K

L

L

E

E

Q

E

R

R

E

E

S

A

G

G

K

K

T

T

L

E

E

E

V

V

V

C

S

F

R

R

Q

Q

L

M

G

E

S

A

I

I

I

R

E

K

E

N

L

L

-

S

G

S

V

A

G

D

A

M

F

W

A

N

K

H

A

I

V

V

I

I

A

A

Y

Y

E
|
E

P

P

V

V

W

W

A
|
A

I

I

G
|
G

T
|
T

G

G

L
x
K

T

T

A

A

T

T

P

E

Q

Q

A

A

Q

Q

D

E

V

V

H

H

A

L

A

A

I

V

R

R

A

R

Q

W

L

M

A

E

E

K

N

V

S

S

E

P

-

A

V

V

A

A

Q

K

G

S

V

I

R

R

L

I

Y

Y

G

G

S
|
S

V

V

K

T

A

A

N

N

A

C

V

R

E

T

L

L

F

A

G

K

M

Q

P

P

D

D

I

V

D

D

G

G

G

F

L

L

I

V

G

G

A

A

S

S

L

L

N

K

A

P

D

D

E

-

F

F

G

I

A

E

I

I

C

C

R

N

A

A

active site: N8 (= N9), K10 (= K11), H92 (= H95), E94 (= E97), E165 (= E167), G171 (= G173), S211 (= S212)
binding magnesium ion: E23 (≠ N24), H27 (= H28), A169 (= A171), T172 (= T174), K174 (≠ L176)

6ooiC Crystal structure of triosephosphate isomerase from schistosoma mansoni in complex with 2pg (see paper)
45% identity, 98% coverage: 2:248/251 of query aligns to 8:253/255 of 6ooiC

query
sites
6ooiC

R

R

R

K

T

F

M

F

V

V

A

G

G

G

N
|
N

W

W

K
|
K

M

M

H

N

G

G

T

S

R

R

A

D

S

D

V

N

A

D

E

K

L

L

I

L

N

K

G

L

L

L

R

S

H

E

L

A

A

H

L

F

P

D

S

D

G

N

V

T

D

E

V

V

A

L

V

I

F

A

P

P

C

S

L

V

H

F

I

L

N

H

Q

E

V

I

V

R

D

K

G

S

L

L

K

K

G

-

K

K

S

E

I

I

Q

H

V

V

G

A

A

A

Q

Q

N

N

S

-

A

C

V

Y

E

K

P

V

M

S

Q

K

G

G

A

A

L

F

T

T

G

G

E

E

I

I

S

S

P

P

S

A

Q

M

L

I

V

R

D

D

A

I

G

G

C

C

S

D

Y

W

V

V

L

I

V

L

G

G

H
|
H

S

S

E
|
E

R

R

Q

N

M

I

M

F

G

G

E

E

R

S

D

D

G

E

T

L

L

I

N

A

R

E

K

K

F

V

A

Q

A

H

A

A

Q

L

A

A

C

E

G

G

L

L

I

S

P

V

V

I

L

A

C

C

I

I

G

G

E

E

T

T

L

L

E

S

Q

E

R

R

E

E

S

S

G

N

K

K

T

T

L

E

E

E

V

V

V

C

S

V

R

R

Q

Q

L

L

G

K

S

A

I

I

I

A

E

N

E

K

L

I

-

K

G

S

V

A

G

D

A

E

F

W

A

K

K

R

A

V

V

V

I

V

A

A

Y

Y

E
|
E

P

P

V

V

W

W

A

A

I
|
I

G
|
G

T

T

G

G

L

K

T

V

A

A

T

T

P

P

Q

Q

A

A

Q

Q

D

E

V

V

H

H

A

N

A

F

I

L

R

R

A

K

Q

W

L

F

A

K

A

T

E

N

N

A

S

P

E

N

-

G

V

V

A

D

Q

E

G

K

V

I

R

R

L

I

Y

Y

G

G

G
|
G

S
|
S

V

V

K

T

A

A

N

N

A

C

V

K

E

E

L

L

F

A

G

Q

M

Q

P

H

D

D

I

V

D

D

G

G

G

F

L
|
L

I

V

G
|
G

A

A

S

S

L

L

N

K

A

P

D

-

E

E

F

F

G

T

A

E

I

I

C

C

R

K

A

A

active site: N15 (= N9), K17 (= K11), H99 (= H95), E101 (= E97), E172 (= E167), G178 (= G173), S218 (= S212)
binding 2-phosphoglycolic acid: N15 (= N9), K17 (= K11), H99 (= H95), E172 (= E167), I177 (= I172), G178 (= G173), G217 (= G211), S218 (= S212), L237 (= L231), G239 (= G233), G240 (= G234)

3ypiA Electrophilic catalysis in triosephosphase isomerase: the role of histidine-95 (see paper)
45% identity, 94% coverage: 2:237/251 of query aligns to 2:235/247 of 3ypiA

query
sites
3ypiA

R

R

R

T

T

F

M

F

V

V

A

G

G

G

N
|
N

W

F

K
|
K

M

L

H

N

G

G

T

S

R

K

A

Q

S

S

V

I

A

K

E

E

L

I

I

V

N

E

G

R

L

L

R

N

H

T

L

A

A

S

L

I

P

P

S

E

G

N

V

V

D

E

V

V

A

V

V

I

F

C

P

P

C

A

L

T

H

Y

I

L

N

D

Q

Y

V

S

V

V

D

S

G

L

L

V

K

K

G

K

K

P

S

Q

I

V

Q

T

V

V

G

G

A

A

Q

Q

N

N

S

A

A

Y

V

L

E

K

P

-

M

A

Q

S

G

G

A

A

L

F

T

T

G

G

E

E

I

N

S

S

P

V

S

D

Q

Q

L

I

V

K

D

D

A

V

G

G

C

A

S

K

Y

W

V

V

L

I

V

L

G

G

H
x
Q

S

S

E
|
E

R

R

Q

S

M

Y

M

F

G

H

E

E

R

D

D

D

G

K

T

F

L

I

-

A

-

D

N

K

R

T

K

K

F

F

A

A

A

L

A

G

Q

Q

A

G

C

V

G

G

L

V

I

I

P

-

V

-

L

L

C

C

I

I

G

G

E

E

T

T

L

L

E

E

Q

E

R

K

E

K

S

A

G

G

K

K

T

T

L

L

E

D

V

V

S

E

R

R

Q

Q

L

L

G

N

S

A

I

V

I

L

E

E

L

-

G

-

V

V

G

K

A

D

F

W

A

T

K

N

A

V

V

V

I

V

A

A

Y

Y

E
|
E

P

P

V

V

W

W

A

A

I
|
I

G
|
G

T

T

G

G

L

L

T

A

A

A

T

T

P

P

Q

E

Q

D

A

A

Q

Q

D

D

V

I

H

H

A

A

A

S

I

I

R

R

A

K

Q

F

L

L

A

A

A

S

E

K

-

L

N

G

S

D

E

K

V

A

A

A

Q

S

G

E

V

L

R

R

L

I

L

L

Y

Y

G

G

S
|
S

V

A

K

N

A

G

A

S

N

N

A

A

V

V

E

T

L

F

F

K

G

D

M

K

P

A

D

D

I

V

D

D

G

G

G

F

L
|
L

I

V

G
|
G

A

A

S

S

L

L

active site: N9 (= N9), K11 (= K11), Q94 (≠ H95), E96 (= E97), E164 (= E167), G170 (= G173), S210 (= S212)
binding phosphoglycolohydroxamic acid: N9 (= N9), K11 (= K11), Q94 (≠ H95), E164 (= E167), I169 (= I172), G170 (= G173), S210 (= S212), L229 (= L231), G231 (= G233), G232 (= G234)

4ff7B Structure of c126s mutant of saccharomyces cerevisiae triosephosphate isomerase (see paper)
45% identity, 94% coverage: 2:237/251 of query aligns to 2:235/247 of 4ff7B

query
sites
4ff7B

R

R

R

T

T

F

M

F

V

V

A

G

G

G

N
|
N

W

F

K
|
K

M

L

H

N

G

G

T

S

R

K

A

Q

S

S

V

I

A

K

E

E

L

I

I

V

N

E

G

R

L

L

R

N

H

T

L

A

A

S

L

I

P

P

S

E

G

N

V

V

D

E

V

V

A

V

V

I

F

C

P

P

C

A

L

T

H

Y

I

L

N

D

Q

Y

V

S

V

V

D

S

G

L

L

V

K

K

G

K

K

P

S

Q

I

V

Q

T

V

V

G

G

A

A

Q

Q

N

N

S

A

A

Y

V

L

E
x
K

P

-

M
x
A

Q
x
S

G

G

A

A

L

F

T

T

G

G

E

E

I

N

S

S

P

V

S

D

Q

Q

L

I

V

K

D

D

A

V

G

G

C

A

S
x
K

Y
x
W

V

V

L

I

V

L

G

G

H
|
H

S

S

E
|
E

R

R

Q

S

M

Y

M

F

G

H

E

E

R

D

D

D

G

K

T

F

L

I

-

A

-

D

N

K

R

T

K

K

F
|
F

A

A

A

L

A

G

Q

Q

A

G

C

V

G
|
G

L

V

I

I

P

-

V

-

L

L

C

S

I

I

G

G

E

E

T

T

L

L

E

E

Q

E

R

K

E

K

S

A

G

G

K

K

T

T

L

L

E

D

V

V

S

E

R

R

Q

Q

L

L

G

N

S

A

I

V

I

L

E

E

L

-

G

-

V

V

G

K

A

D

F

W

A

T

K
x
N

A

V

V

V

I

V

A

A

Y

Y

E
|
E

P

P

V

V

W

W

A

A

I

I

G
|
G

T

T

G

G

L

L

T

A

A

A

T

T

P

P

Q

E

Q

D

A

A

Q

Q

D

D

V

I

H

H

A

A

A

S

I

I

R

R

A

K

Q

F

L

L

A

A

A

S

E

K

-

L

N

G

S

D

E

K

V

A

A

A

Q

S

G

E

V

L

R

R

L

I

L

L

Y

Y

G

G

S
|
S

V

A

K

N

A

G

A

S

N

N

A

A

V

V

E

T

L

F

F

K

G

D

M

K

P

A

D

D

I

V

D

D

G

G

G

F

L

L

I

V

G

G

A

A

S

S

L

L

active site: N9 (= N9), K11 (= K11), H94 (= H95), E96 (= E97), E164 (= E167), G170 (= G173), S210 (= S212)
binding phosphate ion: K68 (≠ E68), A69 (≠ M70), S70 (≠ Q71), K88 (≠ S89), W89 (≠ Y90), F114 (= F113), G121 (= G120), N158 (≠ K161)

4ff7A Structure of c126s mutant of saccharomyces cerevisiae triosephosphate isomerase (see paper)
45% identity, 94% coverage: 2:237/251 of query aligns to 2:235/247 of 4ff7A

query
sites
4ff7A

R

R

R

T

T

F

M

F

V

V

A

G

G

G

N
|
N

W

F

K
|
K

M

L

H

N

G

G

T

S

R

K

A

Q

S

S

V

I

A

K

E

E

L

I

I

V

N

E

G

R

L

L

R

N

H

T

L

A

A

S

L

I

P

P

S

E

G

N

V

V

D

E

V

V

A

V

V

I

F

C

P

P

C

A

L

T

H

Y

I

L

N

D

Q

Y

V

S

V

V

D

S

G

L

L

V

K

K

G

K

K

P

S

Q

I

V

Q

T

V

V

G

G

A

A

Q

Q

N

N

S

A

A

Y

V

L

E

K

P

-

M

A

Q

S

G

G

A

A

L

F

T

T

G

G

E

E

I

N

S

S

P

V

S

D

Q

Q

L

I

V

K

D

D

A

V

G

G

C

A

S

K

Y

W

V

V

L

I

V

L

G

G

H
|
H

S

S

E
|
E

R

R

Q

S

M

Y

M

F

G

H

E

E

R

D

D

D

G

K

T

F

L

I

-

A

-

D

N

K

R

T

K

K

F

F

A

A

A

L

A

G

Q

Q

A

G

C

V

G

G

L

V

I

I

P

-

V

-

L

L

C

S

I

I

G

G

E

E

T

T

L

L

E

E

Q

E

R

K

E

K

S

A

G

G

K

K

T

T

L

L

E

D

V

V

S

E

R

R

Q

Q

L

L

G

N

S

A

I

V

I

L

E

E

L

-

G

-

V

V

G

K

A

D

F

W

A

T

K

N

A

V

V

V

I

V

A

A

Y

Y

E
|
E

P

P

V

V

W

W

A

A

I
|
I

G
|
G

T

T

G

G

L

L

T

A

A

A

T

T

P

P

Q

E

Q

D

A

A

Q

Q

D

D

V

I

H

H

A

A

A

S

I

I

R

R

A

K

Q

F

L

L

A

A

A

S

E

K

-

L

N

G

S

D

E

K

V

A

A

A

Q

S

G

E

V

L

R

R

L

I

L

L

Y

Y

G

G

G
|
G

S
|
S

V

A

K

N

A

G

A

S

N

N

A

A

V

V

E

T

L

F

F

K

G

D

M

K

P

A

D

D

I

V

D

D

G

G

G

F

L

L

I

V

G
|
G

A

A

S

S

L

L

active site: N9 (= N9), K11 (= K11), H94 (= H95), E96 (= E97), E164 (= E167), G170 (= G173), S210 (= S212)
binding 2-phosphoglycolic acid: K11 (= K11), H94 (= H95), E164 (= E167), I169 (= I172), G170 (= G173), G209 (= G211), S210 (= S212), G231 (= G233), G232 (= G234)

P27876 Triosephosphate isomerase; TIM; TPI; Triose-phosphate isomerase; EC 5.3.1.1 from Bacillus subtilis (strain 168) (see paper)
44% identity, 96% coverage: 1:242/251 of query aligns to 1:243/253 of P27876

query
sites
P27876

M

M

R

R

R

K

T

P

M

I

V

I

A

A

G

G

N

N

W

W

K

K

M

M

H

N

G

K

T

T

R

L

A

G

S

E

V

A

A

V

E

S

L

F

I

V

N

E

G

E

L

V

R

K

H

S

L

-

A

S

L

I

P

P

S

A

G

A

-

D

-

K

V

A

D

E

V

A

A

V

V

V

F

C

P

A

P

P

C

A

L

L

H

F

I

L

N

E

Q

K

V

L

V

A

D

S

G

A

L

V

K

K

G

G

K

T

S

D

I

L

Q

K

V

V

G

G

A

A

Q

Q

N

N

S

M

A

H

V

F

E

E

P

E

M

-

Q

S

G

G

A

A

L

F

T

T

G

G

E

E

I

I

S

S

P

P

S

V

Q

A

L

L

V

K

D

D

A

L

G

G

C

V

S

D

Y

Y

V

C

L

V

V

I

G

G

H

H

S

S

E

E

R

R

Q

E

M

M

M

F

G

A

E

E

R

T

D

D

G

E

T

T

L

V

N

N

R

K

K

K

F

A

A

H

A

A

Q

F

A

K

C

H

G

G

L

I

I

V

P

P

V

I

L

I

C

C

I

V

G

G

E

E

T

T

L

L

E

E

Q

E

R

R

E

E

S

A

G

G

K

K

T

T

L

N

E

D

V

L

V

V

S

A

R

D

Q

Q

L

V

G

K

S

K

I

G

I

L

E

A

E

G

L

L

G

S

V

E

G

E

A

Q

F

V

A

A

K

A

A

S

V

V

I

I

A

A

Y

Y

E

E

P

P

V

I

W

W

A

A

I

I

G

G

T

T

G

G

L

K

T

S

A

S

T

T

P

A

Q

K

Q

D

A

A

Q

N

D

D

V

V

H

C

A

A

A

H

I

I

R

R

A

K

Q

T

L

V

A

A

A

E

E

S

N

F

S

S

-

Q

E

E

V

A

A

A

Q

D

G

K

V

L

R

R

L

I

L

Q

Y

Y

G

G

S
|
S

V

V

K

K

A

P

A

A

N

N

A

I

V

K

E

E

L

Y

F

M

G

A

M

E

P

S

D

D

I

I

D

D

G

G

G

A

L

L

I

V

G

G

A

A

S

S

L

L

N

E

A

P

D

Q

E

S

F

F

S213 (= S212) modified: Phosphoserine

1htiB Crystal structure of recombinant human triosephosphate isomerase at 2.8 angstroms resolution. Triosephosphate isomerase related human genetic disorders and comparison with the trypanosomal enzyme (see paper)
47% identity, 98% coverage: 2:248/251 of query aligns to 4:246/248 of 1htiB

query
sites
1htiB

R

R

R

K

T

F

M

F

V

V

A

G

G

G

N
|
N

W

W

K
|
K

M

M

H

N

G

G

T

R

R

K

A

Q

S

S

V

L

A

G

E

E

L

L

I

I

N

G

G

T

L

L

R

N

H

A

L

A

A

K

L

V

P

P

S

A

G

D

V

T

D

E

V

V

A

V

V

C

F

A

P

P

C

T

L

A

H

Y

I

I

N

D

Q

F

V

A

V

R

D

Q

G

K

L

L

K

D

G

P

K

K

S

-

I

I

Q

A

V

V

G

A

A

A

Q

Q

N

N

S

-

A

C

V

Y

E

K

P

V

M

T

Q

N

G

G

A

A

L

F

T

T

G

G

E

E

I

I

S

S

P

P

S

G

Q

M

L

I

V

K

D

D

A

C

G

G

C

A

S

T

Y

W

V

V

L

V

V

L

G

G

H
|
H

S

S

E
|
E

R

R

Q

H

M

V

M

F

G

G

E

E

R

S

D

D

G

E

T

L

L

I

N

G

R

Q

K

K

F

V

A

A

A

H

A

A

Q

L

A

A

C

E

G

G

L

L

I

G

P

V

V

I

L

A

C

C

I

I

G

G

E

E

T

K

L

L

E

D

Q

E

R

R

E

E

S

A

G

G

K

I

T

T

L

E

E

K

V

V

S

F

R

E

Q

Q

L

T

G

K

S

V

I

I

I

A

E

D

E

-

L

-

G

N

V

V

G

K

A

D

F

W

A

S

K

K

A

V

V

V

I

L

A

A

Y

Y

E
|
E

P

P

V

V

W

W

A
|
A

I
|
I

G
|
G

T

T

G

G

L

K

T

T

A

A

T

T

P

P

Q

Q

A

A

Q

Q

D

E

V

V

H

H

A

E

A

K

I

L

R

R

A

G

Q

W

L

L

A

K

A

S

E

N

N

V

S

S

E

D

-

A

V

V

A

A

Q

Q

G

S

V

T

R

R

L

I

Y

Y

G

G

G
|
G

S
|
S

V

V

K

T

A

G

A

A

N

T

A

C

V

K

E

E

L

L

F

A

G

S

M

Q

P

P

D

D

I

V

D

D

G

G

G

F

L
|
L

I
x
V

G
|
G

A

A

S

S

L

L

N

K

A

P

D

-

E

E

F

F

G

V

A

D

I

I

C

I

R

N

A

A

active site: N11 (= N9), K13 (= K11), H95 (= H95), E97 (= E97), E165 (= E167), G171 (= G173), S211 (= S212)
binding 2-phosphoglycolic acid: N11 (= N9), K13 (= K11), H95 (= H95), E165 (= E167), A169 (= A171), I170 (= I172), G171 (= G173), G210 (= G211), S211 (= S212), L230 (= L231), V231 (≠ I232), G232 (= G233), G233 (= G234)

P60174 Triosephosphate isomerase; TIM; Methylglyoxal synthase; Triose-phosphate isomerase; EC 5.3.1.1; EC 4.2.3.3 from Homo sapiens (Human) (see 7 papers)
47% identity, 98% coverage: 2:248/251 of query aligns to 5:247/249 of P60174

query
sites
P60174

R

R

R

K

T

F

M

F

V

V

A

G

G

G

N
|
N

W

W

K
|
K

M

M

H

N

G

G

T

R

R

K

A

Q

S

S

V

L

A

G

E

E

L

L

I

I

N

G

G

T

L

L

R

N

H

A

L

A

A

K

L

V

P

P

S

A

G

D

V

T

D

E

V

V

A

V

V

C

F

A

P

P

C

T

L

A

H

Y

I

I

N

D

Q

F

V

A

V

R

D

Q

G

K

L

L

K

D

G

P

K

K

S

-

I

I

Q

A

V

V

G

A

A

A

Q

Q

N

N

S

-

A

C

V

Y

E

K

P

V

M

T

Q

N

G

G

A

A

L

F

T

T

G

G

E

E

I

I

S

S

P

P

S

G

Q

M

L

I

V

K

D

D

A

C

G

G

C

A

S

T

Y

W

V

V

L

V

V

L

G

G

H
|
H

S

S

E

E

R

R

Q

H

M

V

M

F

G

G

E
|
E

R

S

D

D

G

E

T

L

L

I

N

G

R

Q

K

K

F

V

A

A

A

H

A

A

Q

L

A

A

C

E

G

G

L

L

I
x
G

P

V

V

I

L

A

C

C

I

I

G

G

E

E

T

K

L

L

E

D

Q

E

R

R

E

E

S

A

G

G

K

I

T

T

L

E

E

K

V

V

S

F

R

E

Q

Q

L

T

G

K

S

V

I

I

I

A

E

D

E

-

L

-

G

N

V

V

G

K

A

D

F

W

A

S

K

K

A

V

V

V

I

L

A

A

Y

Y

E
|
E

P

P

V

V

W

W

A

A

I

I

G

G

T

T

G

G

L

K

T

T

A

A

T

T

P

P

Q

Q

A

A

Q

Q

D

E

V

V

H

H

A

E

A

K

I

L

R

R

A

G

Q

W

L

L

A

K

A

S

E

N

N

V

S

S

E

D

-

A

V

V

A

A

Q

Q

G

S

V

T

R

R

L

I

Y

Y

G

G

S

S

V

V

K

T

A

G

A

A

N

T

A

C

V

K

E

E

L

L

F

A

G

S

M

Q

P

P

D

D

I

V

D

D

G

G

G

F

L

L

I

V

G

G

A

A

S

S

L

L

N

K

A

P

D

-

E

E

F
|
F

G

V

A

D

I

I

C

I

R

N

A

A

N12 (= N9) binding
K14 (= K11) binding
H96 (= H95) active site, Electrophile
E105 (= E104) to D: in TPID; no effect on triose-phosphate isomerase activity; changed protein homodimerization activity; the homodimer stability is temperature-dependent and affects the triose-phosphate isomerase activity; dbSNP:rs121964845
G123 (≠ I122) to R: in Manchester; thermolabile; dbSNP:rs121964846
E166 (= E167) active site, Proton acceptor
F241 (= F242) to L: in TPID; Hungary; thermolabile; dbSNP:rs121964847

4pocB Structure of triosephosphate isomerase wild type human enzyme. (see paper)
47% identity, 98% coverage: 2:248/251 of query aligns to 3:245/247 of 4pocB

query
sites
4pocB

R

R

R

K

T

F

M

F

V

V

A

G

G

G

N
|
N

W

W

K
|
K

M

M

H

N

G

G

T

R

R

K

A

Q

S

S

V

L

A

G

E

E

L

L

I

I

N

G

G

T

L

L

R

N

H

A

L

A

A

K

L

V

P

P

S

A

G

D

V

T

D

E

V

V

A

V

V

C

F

A

P

P

C

T

L

A

H

Y

I

I

N

D

Q

F

V

A

V

R

D

Q

G

K

L

L

K

D

G

P

K

K

S

-

I

I

Q

A

V

V

G

A

A

A

Q

Q

N

N

S

-

A

C

V

Y

E

K

P

V

M

T

Q

N

G

G

A

A

L

F

T

T

G

G

E

E

I

I

S

S

P

P

S

G

Q

M

L

I

V

K

D

D

A

C

G

G

C

A

S

T

Y

W

V

V

L

V

V

L

G

G

H
|
H

S

S

E
|
E

R

R

Q

H

M

V

M

F

G

G

E

E

R

S

D

D

G

E

T

L

L

I

N

G

R

Q

K

K

F

V

A

A

A

H

A

A

Q

L

A

A

C

E

G

G

L

L

I

G

P

V

V

I

L

A

C

C

I

I

G

G

E

E

T

K

L

L

E

D

Q

E

R

R

E

E

S

A

G

G

K

I

T

T

L

E

E

K

V

V

S

F

R

E

Q

Q

L

T

G

K

S

V

I

I

I

A

E

D

E

-

L

-

G

N

V

V

G

K

A

D

F

W

A

S

K

K

A

V

V

V

I

L

A

A

Y

Y

E
|
E

P

P

V

V

W

W

A

A

I
|
I

G
|
G

T

T

G

G

L

K

T

T

A

A

T

T

P

P

Q

Q

A

A

Q

Q

D

E

V

V

H

H

A

E

A

K

I

L

R

R

A

G

Q

W

L

L

A

K

A

S

E

N

N

V

S

S

E

D

-

A

V

V

A

A

Q

Q

G

S

V

T

R

R

L

I

Y

Y

G

G

G
|
G

S
|
S

V

V

K

T

A

G

A

A

N

T

A

C

V

K

E

E

L

L

F

A

G

S

M

Q

P

P

D

D

I

V

D

D

G

G

G

F

L

L

I

V

G
|
G

A

A

S

S

L

L

N

K

A

P

D

-

E

E

F

F

G

V

A

D

I

I

C

I

R

N

A

A

active site: N10 (= N9), K12 (= K11), H94 (= H95), E96 (= E97), E164 (= E167), G170 (= G173), S210 (= S212)
binding phosphate ion: K12 (= K11), I169 (= I172), G170 (= G173), G209 (= G211), S210 (= S212), G231 (= G233), G232 (= G234)