SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF5025 FitnessBrowser__WCS417:GFF5025 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 3 hits to proteins with known functional sites (download)

P68183 Maltose/maltodextrin transport system permease protein MalG from Escherichia coli (strain K12) (see 2 papers)
27% identity, 86% coverage: 28:262/274 of query aligns to 27:285/296 of P68183

query
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P68183

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E190 (≠ R171) mutation E->A,C,K,L: Reduction of transport rate.
A192 (= A173) mutation A->D,S,L: Loss of transport and MalK dissociation from the membrane.
G196 (= G177) mutation to A: No effect.; mutation to P: Loss of transport and MalK dissociation from the membrane.
P209 (= P190) mutation to A: No effect.

P02916 Maltose/maltodextrin transport system permease protein MalF from Escherichia coli (strain K12) (see 4 papers)
27% identity, 44% coverage: 103:222/274 of query aligns to 313:454/514 of P02916

query
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P02916

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L334 (vs. gap) mutation to W: Ability to transport lactose in a saturable manner.
L372 (= L142) mutation to W: Growth on maltose but not on media containing either maltoheptaose or maltoheptaose plus maltose.
N376 (≠ H146) mutation N->K,H: No growth on maltose.
G380 (= G150) mutation G->C,S: No growth on maltose.
E401 (≠ R171) mutation E->A,C,K,L: Reduction of transport rate.
S403 (≠ A173) mutation S->C,D,K,L: Reduction of transport rate.
G407 (= G177) mutation G->A,P: No effect.
P420 (= P190) mutation to A: No effect.

4ki0F Crystal structure of the maltose-binding protein/maltose transporter complex in an outward-facing conformation bound to maltohexaose (see paper)
27% identity, 44% coverage: 103:222/274 of query aligns to 298:439/490 of 4ki0F

query
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4ki0F

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binding alpha-D-glucopyranose: Y310 (≠ M115), N361 (≠ H146), G365 (= G150), Y368 (≠ F153), S418 (≠ A203), N422 (≠ S207)

Query Sequence

>GFF5025 FitnessBrowser__WCS417:GFF5025
MLSPYMSPVERVWFYSLRILCGLILLFLILPVLVIIPLSFNSGSFLVYPLQGFSLHWYQD
FFASAEWMRALKNSVIVAPAATVLAMVFGTLAAIGLTRGNFPGKALVMALVISPMVVPVV
IIGVASYLFFAPLGLGNSFFSLIVVHAVLGVPFVIITVSATLQGFNHNLVRAAASLGASP
LTAFRRVTLPLIAPGVISGALFAFSTSFDEVVVTLFLAGPEQATLPRQMFSGIRENLSPT
IAAAATLLIAFSVLLLLTLEWLRGRSEKLRTAQV

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory