Comparing GFF5059 FitnessBrowser__WCS417:GFF5059 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
4iztA The e41q mutant of the amidase from nesterenkonia sp. An1 showing covalent addition of the acetamide moiety of fluoroacetamide at the active site cysteine
35% identity, 98% coverage: 1:258/264 of query aligns to 10:259/263 of 4iztA
4izuA The e41q mutant of the amidase from nesterenkonia sp. An1 showing the result of michael addition of acrylamide at the active site cysteine
34% identity, 98% coverage: 1:258/264 of query aligns to 2:250/254 of 4izuA
5nybA A c145a mutant of nesterenkonia an1 amidase bound to adipamide
35% identity, 98% coverage: 1:258/264 of query aligns to 9:258/262 of 5nybA
5ny7A A c145a mutant of nesterenkonia an1 amidase bound to nicotinamide
35% identity, 98% coverage: 1:258/264 of query aligns to 9:258/262 of 5ny7A
Sites not aligning to the query:
5nycA A c145a mutant of nesterenkonia an1 amidase bound to propionitrile
34% identity, 98% coverage: 1:258/264 of query aligns to 9:257/261 of 5nycA
4izsA The c145a mutant of the amidase from nesterenkonia sp. An1 in complex with butyramide
34% identity, 98% coverage: 1:258/264 of query aligns to 9:257/261 of 4izsA
3klcB Crystal structure of hyperthermophilic nitrilase (see paper)
33% identity, 98% coverage: 1:258/264 of query aligns to 1:259/261 of 3klcB
Sites not aligning to the query:
3klcA Crystal structure of hyperthermophilic nitrilase (see paper)
33% identity, 98% coverage: 1:258/264 of query aligns to 1:259/261 of 3klcA
Q9UYV8 Nitrilase; PaNit; EC 3.5.5.1 from Pyrococcus abyssi (strain GE5 / Orsay) (see paper)
33% identity, 98% coverage: 1:258/264 of query aligns to 2:260/262 of Q9UYV8
6ypaB The c146a variant of an amidase from pyrococcus horikoshii with bound glutaramide
31% identity, 98% coverage: 1:258/264 of query aligns to 9:267/269 of 6ypaB
7ovgA The c146a variant of an amidase from pyrococcus horikoshii with bound acetamide (see paper)
31% identity, 98% coverage: 1:258/264 of query aligns to 3:261/263 of 7ovgA
Q9NQR4 Omega-amidase NIT2; Nitrilase homolog 2; EC 3.5.1.3 from Homo sapiens (Human) (see 2 papers)
29% identity, 100% coverage: 1:264/264 of query aligns to 4:276/276 of Q9NQR4
5h8jB Crystal structure of medicago truncatula n-carbamoylputrescine amidohydrolase (mtcpa) in complex with cadaverine (see paper)
30% identity, 94% coverage: 14:261/264 of query aligns to 17:287/297 of 5h8jB
5h8iC Crystal structure of medicago truncatula n-carbamoylputrescine amidohydrolase (mtcpa) in complex with n-(dihydroxymethyl)putrescine (see paper)
30% identity, 94% coverage: 14:261/264 of query aligns to 21:291/301 of 5h8iC
5h8lB Crystal structure of medicago truncatula n-carbamoylputrescine amidohydrolase (mtcpa) c158s mutant in complex with putrescine (see paper)
30% identity, 94% coverage: 14:261/264 of query aligns to 18:288/298 of 5h8lB
Q44185 N-carbamoyl-D-amino acid hydrolase; D-N-alpha-carbamilase; EC 3.5.1.77 from Rhizobium radiobacter (Agrobacterium tumefaciens) (Agrobacterium radiobacter) (see paper)
27% identity, 88% coverage: 29:261/264 of query aligns to 37:301/304 of Q44185
Q94JV5 Deaminated glutathione amidase, chloroplastic/cytosolic; dGSH amidase; Nitrilase-like protein 2; Protein nitrilase 1 homolog; AtNit1; Protein Nit1 homolog; EC 3.5.1.128 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
29% identity, 88% coverage: 14:245/264 of query aligns to 49:294/307 of Q94JV5
Sites not aligning to the query:
8hpcC Crystal structure of c171a mutant of n-carbamyl-d-amino acid amidohydrolase complexed with n-carbamyl-d-hydroxyphenylglycine
28% identity, 88% coverage: 29:261/264 of query aligns to 36:300/303 of 8hpcC
1uf8A Crystal structure of c171a/v236a mutant of n-carbamyl-d-amino acid amidohydrolase complexed with n-carbamyl-d-phenylalanine
28% identity, 88% coverage: 29:261/264 of query aligns to 36:300/303 of 1uf8A
1uf7A Crystal structure of c171a/v236a mutant of n-carbamyl-d-amino acid amidohydrolase complexed with n-carbamyl-d-valine
28% identity, 88% coverage: 29:261/264 of query aligns to 36:300/303 of 1uf7A
>GFF5059 FitnessBrowser__WCS417:GFF5059
MRVALYQCPPQPLDVSGNLKRLHALAHEASSADVLVLPEMFLTGYNIGAEAVGALAEAQD
GPSAQAIAELAKSAGLAILYGYPERAEDGQIYNAVQLIDAHGQCRCNYRKTHLFGDLDHS
MFSAGEDNFPLVELNGWTLGFLICYDLEFPENTRRLALAGAELILVPTANMVPFDFVADV
TVRARAFENQCYVAYANYCGREGEIQYCGQSSIAAPNGQRIAQAGLDEALIVGELDRQSI
LDARAANHYLQDRRPELYGALHKP
Or try a new SitesBLAST search
SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.
Lawrence Berkeley National Laboratory