SitesBLAST
Comparing GFF5065 FitnessBrowser__WCS417:GFF5065 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
Q9X2A5 Acetylornithine aminotransferase; ACOAT; EC 2.6.1.11 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8)
41% identity, 87% coverage: 28:394/420 of query aligns to 3:372/385 of Q9X2A5
- GT 94:95 (≠ GA 119:120) binding pyridoxal 5'-phosphate
- T268 (= T292) binding pyridoxal 5'-phosphate
2ordA Crystal structure of acetylornithine aminotransferase (ec 2.6.1.11) (acoat) (tm1785) from thermotoga maritima at 1.40 a resolution
41% identity, 87% coverage: 28:394/420 of query aligns to 11:380/393 of 2ordA
- active site: F134 (≠ S151), E186 (= E202), D219 (= D235), Q222 (= Q238), K248 (= K264), T276 (= T292), R367 (= R381)
- binding pyridoxal-5'-phosphate: G102 (= G119), T103 (≠ A120), F134 (≠ S151), H135 (= H152), E186 (= E202), D219 (= D235), V221 (= V237), Q222 (= Q238), K248 (= K264)
2eh6A Crystal structure of acetylornithine aminotransferase from aquifex aeolicus vf5
42% identity, 87% coverage: 28:394/420 of query aligns to 3:365/375 of 2eh6A
- active site: F127 (≠ S151), E179 (= E202), D212 (= D235), Q215 (= Q238), K241 (= K264), T270 (= T292), R352 (= R381)
- binding pyridoxal-5'-phosphate: G95 (= G119), T96 (≠ A120), F127 (≠ S151), H128 (= H152), E179 (= E202), D212 (= D235), V214 (= V237), K241 (= K264)
O66442 Acetylornithine aminotransferase; ACOAT; EC 2.6.1.11 from Aquifex aeolicus (strain VF5)
42% identity, 87% coverage: 28:394/420 of query aligns to 4:366/376 of O66442
- GT 96:97 (≠ GA 119:120) binding pyridoxal 5'-phosphate
- K242 (= K264) modified: N6-(pyridoxal phosphate)lysine
- T271 (= T292) binding pyridoxal 5'-phosphate
P73133 Acetylornithine aminotransferase; ACOAT; EC 2.6.1.11 from Synechocystis sp. (strain ATCC 27184 / PCC 6803 / Kazusa) (see paper)
38% identity, 95% coverage: 8:405/420 of query aligns to 14:426/429 of P73133
- Y39 (≠ V32) mutation to F: Retains 7.4% of N-acetylornithine aminotransferase wild-type activity.
- S125 (= S118) mutation to A: Retains 14% of N-acetylornithine aminotransferase wild-type activity.
- G126 (= G119) mutation to A: Loss of N-acetylornithine aminotransferase activity.
- A127 (= A120) mutation to S: Retains 9.1% of N-acetylornithine aminotransferase wild-type activity.
- R163 (= R154) mutation to A: 4100-fold increase in KM for acetylornithine, but does not affect KM for 2-oxoglutarate. Retains 9.5% of N-acetylornithine aminotransferase wild-type activity.
- E223 (= E207) mutation to A: 65-fold increase in KM for acetylornithine, but does not affect KM for 2-oxoglutarate. Retains 50% of N-acetylornithine aminotransferase wild-type activity.; mutation to S: 73-fold increase in KM for acetylornithine, but does not affect KM for 2-oxoglutarate. Shows 160% of N-acetylornithine aminotransferase wild-type activity.
- D251 (= D235) mutation to A: Loss of N-acetylornithine aminotransferase activity.; mutation to E: Retains 20% of N-acetylornithine aminotransferase wild-type activity.
- Q254 (= Q238) mutation to A: Retains 0.9% of N-acetylornithine aminotransferase wild-type activity.
- K280 (= K264) mutation to A: Loss of N-acetylornithine aminotransferase activity.
- T308 (= T292) mutation to A: Retains 0.3% of N-acetylornithine aminotransferase wild-type activity.
- R402 (= R381) mutation to A: 2080-fold increase in KM for acetylornithine, but does not affect KM for 2-oxoglutarate. Retains 18% of N-acetylornithine aminotransferase wild-type activity.
4addA Structural and functional study of succinyl-ornithine transaminase from e. Coli (see paper)
38% identity, 90% coverage: 26:403/420 of query aligns to 10:394/400 of 4addA
- active site: F136 (≠ S151), E188 (= E202), D221 (= D235), Q224 (= Q238), K250 (= K264), T279 (= T292), R372 (= R381)
- binding pyridoxal-5'-phosphate: G103 (= G119), A104 (= A120), F136 (≠ S151), H137 (= H152), D221 (= D235), V223 (= V237), K250 (= K264)
- binding n~2~-(3-carboxypropanoyl)-l-ornithine: Y16 (≠ V32), F136 (≠ S151), R139 (= R154)
4adbB Structural and functional study of succinyl-ornithine transaminase from e. Coli (see paper)
38% identity, 90% coverage: 26:403/420 of query aligns to 10:394/401 of 4adbB
- active site: F136 (≠ S151), E188 (= E202), D221 (= D235), Q224 (= Q238), K250 (= K264), T279 (= T292), R372 (= R381)
- binding pyridoxal-5'-phosphate: S102 (= S118), G103 (= G119), A104 (= A120), F136 (≠ S151), H137 (= H152), D221 (= D235), V223 (= V237), Q224 (= Q238), K250 (= K264)
8ht4B Crystal structure of acetylornithine aminotransferase complex with plp from corynebacterium glutamicum (see paper)
37% identity, 88% coverage: 35:405/420 of query aligns to 17:390/390 of 8ht4B
4jevB N-acetylornithine aminotransferase from s. Typhimurium complexed with gabaculine
34% identity, 93% coverage: 18:407/420 of query aligns to 2:398/402 of 4jevB
- active site: F136 (≠ S151), E188 (= E202), D221 (= D235), Q224 (= Q238), K250 (= K264), T279 (= T292), R372 (= R381)
- binding 3-[o-phosphonopyridoxyl]--amino-benzoic acid: I46 (≠ N62), S102 (= S118), G103 (= G119), T104 (≠ A120), F136 (≠ S151), H137 (= H152), E188 (= E202), E193 (= E207), D221 (= D235), V223 (= V237), Q224 (= Q238), K250 (= K264), R372 (= R381)
P40732 Acetylornithine/succinyldiaminopimelate aminotransferase; ACOAT; DapATase; Succinyldiaminopimelate transferase; EC 2.6.1.11; EC 2.6.1.17 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
34% identity, 93% coverage: 18:407/420 of query aligns to 7:403/405 of P40732
- GT 108:109 (≠ GA 119:120) binding pyridoxal 5'-phosphate
- K255 (= K264) modified: N6-(pyridoxal phosphate)lysine
- T284 (= T292) binding pyridoxal 5'-phosphate
A0QYS9 Acetylornithine aminotransferase; ACOAT; EC 2.6.1.11 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
37% identity, 89% coverage: 28:400/420 of query aligns to 11:379/390 of A0QYS9
- K304 (≠ L324) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
4jewA N-acetylornithine aminotransferase from s. Typhimurium complexed with l-canaline
34% identity, 93% coverage: 18:407/420 of query aligns to 2:393/397 of 4jewA