SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF5251 FitnessBrowser__WCS417:GFF5251 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 15 hits to proteins with known functional sites (download)

P9WKJ5 Dihydroxy-acid dehydratase; DAD; EC 4.2.1.9 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
39% identity, 98% coverage: 5:604/613 of query aligns to 20:573/575 of P9WKJ5

query
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P9WKJ5

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C64 (≠ G49) binding [2Fe-2S] cluster
D96 (= D81) binding Mg(2+)
C137 (= C122) binding [2Fe-2S] cluster
D138 (= D123) binding Mg(2+)
K139 (= K124) binding via carbamate group; modified: N6-carboxylysine
C214 (= C193) binding [2Fe-2S] cluster
E465 (= E489) binding Mg(2+)
S491 (= S515) mutation to A: Loss of catalytic activity.

6ovtA Crystal structure of ilvd from mycobacterium tuberculosis (see paper)
39% identity, 98% coverage: 5:604/613 of query aligns to 7:560/562 of 6ovtA

query
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6ovtA

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binding fe2/s2 (inorganic) cluster: C51 (≠ G49), G84 (= G82), C124 (= C122), D125 (= D123), A200 (≠ S192), C201 (= C193)
binding magnesium ion: D83 (= D81), D125 (= D123), K126 (= K124), E452 (= E489)

Q9LIR4 Dihydroxy-acid dehydratase, chloroplastic; AthDHAD; DAD; EC 4.2.1.9 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
37% identity, 99% coverage: 4:607/613 of query aligns to 56:608/608 of Q9LIR4

query
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Q9LIR4

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L

L

G

S

Q

E

L

A

V

V

A

K

R

E

E

G

I

V

E

E

R

N

A

A

G

G

G

M

V

V

A

G

K

F

E

R

F

F

N

N

T

T

I

I

A

G

V

V

D

S

D

D

G

A

I

I

A

S

M

M

G

G

H

T

D

R

G

G

M

M

L

C

Y

F

S

S

L

L

P

Q

S

S

R

R

E

D

I

L

I

I

A

A

D

D

S

S

V

I

E

E

Y

T

M

V

V

M

N

S

A

A

H

Q

C

W

A

Y

D

D

A

G

I

N

V

I

C

S

I

I

S

P

N

G

C
|
C

D
|
D

K

K

I

N

T

M

P

P

G

G

M

T

L

I

M

M

A

A

A

M

L

G

R

R

L

L

N

N

I

R

P

P

V

G

I

I

F

M

V

V

S

Y

G

G

G

G

P

T

M

I

E

K

A

P

G

G

K

H

T

F

K

Q

L

D

A

K

S

T

H

Y

G

-

L

-

D

D

L

I

V

V

D

S

A

A

M

F

V

Q

I

S

A

Y

A

G

D

E

-

F

-

V

-

S

S

G

S

S

A

I

S

S

D

D

E

E

K

Q

V

R

A

K

E

T

Y

V

E

L

R

H

S

H

A

S

C

C

P

P

T

G

C

A

G

G

S

A

C
|
C

S

G

G

G

M

M

F

Y

T

T

A

A

N

N

S

T

M

M

N

A

C

S

L

A

V

I

E

E

A

A

L

M

G

G

L

M

A

S

L

L

P

P

G

Y

N

S

G

S

S

S

T

I

L

P

A

A

T

E

H

D

S

P

D

L

R

K

E

L

Q

D

L

E

F

C

L

R

Q

L

A

A

G

G

R

K

T

Y

I

L

V

L

E

E

L

L

C

L

K

K

R

M

Y

-

Y

-

G

-

E

-

N

-

D

-

E

-

S

D

V

L

L

K

P

P

R

R

N

D

I

I

A

I

N

T

F

P

K

K

A

S

F

L

E

R

N

N

A

A

M

M

T

V

L

S

D

V

I

M

A

A

M

L

G

G

G

G

S

S

T

T

N

N

T

A

I

V

L

L

H

H

L

L

L

I

A

A

A

I

A

A

Q

R

E

S

A

V

E

G

I

L

E

E

F

L

D

T

L

L

R

D

D

D

I

F

D

Q

R

K

L

V

S

S

R

D

H

A

V

V

P

P

Q

F

L

L

C

A

K

D

V

L

A

K

P

P

N

S

I

-

Q

G

K

K

Y

Y

H

V

M

M

E

E

D

D

V

I

H

H

R

K

A

I

G

G

G

G

I

T

F

P

S

A

I

V

L

L

G

R

S

Y

L

L

A

L

R

E

G

L

G

G

L

L

L

M

H

D

T

G

D

D

L

C

P

M

T

T

V

V

H

T

S

G

K

Q

S

T

I

L

A

A

E

Q

G

N

I

L

A

E

K

N

W

-

D

-

I

-

T

-

Q

-

T

-

D

-

D

-

E

-

A

-

V

-

H

-

T

-

F

-

F

-

K

-

A

-

G

-

P

-

A

-

G

-

I

V

P

P

T

S

Q

L

T

T

A

E

F

G

S

Q

Q

E

S

-

T

-

R

-

W

-

D

-

T

-

L

-

D

-

D

-

D

-

R

-

E

-

N

-

G

-

C

I

I

I

R

R

S

P

V

L

E

S

H

N

A

P

Y

I

S

K

K

E

E

T

G

G

G

H

L

I

A

Q

V

I

L

L

Y

R

G

G

N

D

I

L

A

A

L

P

D

D

G

G

C

S

V

V

V

A

K

K

T

I

A

T

G

G

V

-

D

K

E

E

S

G

I

L

H

Y

V

-

F

F

E

S

G

G

N

P

A

A

K

L

I

V

F

F

E

E

S

G

Q

E

D

E

S

S

A

M

V

L

R

A

G

A

I

I

L

S

A

A

D

D

E

P

V

M

K

S

-

F

A

K

G

G

D

T

I

V

V

V

I

V

I

I

R

R

Y

G

E

E

G

G

P

P

K

K

G

G

G

G

P

P

G

G

M

M

Q

P

E
|
E

M

M

L

L

Y

T

P

P

T

T

S

S

Y

A

L

I

K

M

S

G

K

A

G

G

L

L

G

G

K

K

A

E

C

C

A

A

L

L

L

L

T

T

D

D

G

G

R

R

F

F

S

S

G

G

G

G

T

S

S

H

G

G

L

F

S

V

I

V

G

G

H

H

A

I

S

C

P

P

E

E

A

A

A

Q

A

E

G

G

G

G

A

P

I

I

G

G

L

L

V

I

Q

K

D

N

G

G

D

D

K

I

V

I

L

T

I

I

D

D

I

I

P

G

N

K

R

K

S

R

I

I

N

D

L

T

L

Q

I

V

S

S

D

P

E

E

E

E

L

M

A

N

A

D

R

R

R

R

V

-

E

-

Q

-

D

-

K

K

K

K

G

W

W

T

K

A

P

P

V

A

E

Y

K

K

R

V

P

N

R

R

K

G

V

V

T

-

T

-

A

-

L

L

K

Y

A

K

Y

Y

A

I

L

K

L

N

A

V

T

Q

S

S

A

A

D

S

K

D

G

G

A

C

V

V

R

T

N

D

K

E

C100 (≠ G49) binding [2Fe-2S] cluster
C173 (= C122) binding [2Fe-2S] cluster
D174 (= D123) binding Mg(2+)
C245 (= C193) binding [2Fe-2S] cluster
E497 (= E489) binding Mg(2+)

8hs0A The mutant structure of dhad v178w
37% identity, 99% coverage: 4:607/613 of query aligns to 18:570/570 of 8hs0A

query
sites
8hs0A

Y

Y

R

S

S

S

K

R

T

I

S

-

T

T

H

E

G

P

R

K

N

S

M

Q

A

G

G

G

A

S

R

Q

A

A

L

I

W

L

R

H

A

G

T

V

G

G

M

L

K

S

D

D

D

D

D

D

F

L

K

L

K

K

P

P

I

Q

I

I

A

G

I

I

A

S

N

S

S

V

F

W

T

Y

Q

E

F

G

V

N

P

T

G
x
C

H

N

V

M

H

H

L

L

K

L

D

K

L

L

G

S

Q

E

L

A

V

V

A

K

R

E

E

G

I

V

E

E

R

N

A

A

G

G

G

M

V

V

A

G

K

F

E

R

F

F

N

N

T

T

I

I

A

G

V

V

D

S

D
|
D

G
x
A

I

I

A

S

M

M

G

G

H

T

D

R

G

G

M

M

L

C

Y

F

S

S

L

L

P

Q

S

S

R

R

E

D

I

L

I

I

A

A

D

D

S

S

V

I

E

E

Y

T

M

V

V

M

N

S

A

A

H

Q

C

W

A

Y

D

D

A

G

I

N

V

I

C

S

I

I

S

P

N

G

C
|
C

D
|
D

K
|
K

I

N

T

M

P

P

G

G

M

T

L

I

M

M

A

A

A

M

L

G

R

R

L

L

N

N

I

R

P

P

V

G

I

I

F

M

V

V

S

Y

G

G

G

G

P

T

M

I

E

K

A

P

G

G

K

H

T

F

K

Q

L

D

A

K

S

T

H

Y

G

-

L

-

D

D

L

I

V

W

D

S

A

A

M

F

V

Q

I

S

A

Y

A

G

D

E

-

F

-

V

-

S

S

G

S

S

A

I

S

S

D

D

E

E

K

Q

V

R

A

K

E

T

Y

V

E

L

R

H

S

H

A

S

C

C

P

P

T

G

C

A

G

G

S

A

C
|
C

S

G

G

G

M

M

F

Y

T

T

A

A

N

N

S

T

M

M

N

A

C

S

L

A

V

I

E

E

A

A

L

M

G

G

L

M

A

S

L

L

P

P

G

Y

N

S

G

S

S

S

T

I

L

P

A

A

T

E

H

D

S

P

D

L

R

K

E

L

Q

D

L

E

F

C

L

R

Q

L

A

A

G

G

R

K

T

Y

I

L

V

L

E

E

L

L

C

L

K

K

R

M

Y

-

Y

-

G

-

E

-

N

-

D

-

E

-

S

D

V

L

L

K

P

P

R

R

N

D

I

I

A

I

N

T

F

P

K

K

A

S

F

L

E

R

N

N

A

A

M

M

T

V

L

S

D

V

I

M

A

A

M

L

G

G

G

G

S

S

T

T

N
|
N

T

A

I

V

L

L

H
|
H

L

L

L

I

A

A

A

I

A

A

Q

R

E

S

A

V

E

G

I

L

E

E

F

L

D

T

L

L

R

D

D

D

I

F

D

Q

R

K

L

V

S

S

R

D

H

A

V

V

P

P

Q

F

L

L

C

A

K

D

V

L

A

K

P

P

N

S

I

-

Q

G

K

K

Y

Y

H

V

M

M

E

E

D

D

V

I

H

H

R

K

A

I

G

G

G

G

I

T

F

P

S

A

I

V

L

L

G

R

S

Y

L

L

A

L

R

E

G

L

G

G

L

L

L

M

H

D

T

G

D

D

L

C

P

M

T

T

V

V

H

T

S

G

K

Q

S

T

I

L

A

A

E

Q

G

N

I

L

A

E

K

N

W

-

D

-

I

-

T

-

Q

-

T

-

D

-

D

-

E

-

A

-

V

-

H

-

T

-

F

-

F

-

K

-

A

-

G

-

P

-

A

-

G

-

I

V

P

P

T

S

Q

L

T

T

A

E

F

G

S

Q

Q

E

S

-

T

-

R

-

W

-

D

-

T

-

L

-

D

-

D

-

D

-

R

-

E

-

N

-

G

-

C

I

I

I

R

R

S

P

V

L

E

S

H

N

A

P

Y

I

S

K

K

E

E

T

G

G

G

H

L

I

A

Q

V

I

L

L

Y

R

G

G

N

D

I

L

A

A

L

P

D

D

G

G

C

S

V

V

V

A

K

K

T

I

A

T

G

G

V

-

D

K

E

E

S

G

I

L

H

Y

V

-

F

F

E

S

G

G

N

P

A

A

K

L

I

V

F

F

E

E

S

G

Q

E

D

E

S

S

A

M

V

L

R

A

G

A

I

I

L

S

A

A

D

D

E

P

V

M

K

S

-

F

A

K

G

G

D

T

I

V

V

V

I

V

I

I

R

R

Y

G

E

E

G

G

P

P

K

K

G

G

G

G

P

P

G

G

M

M

Q

P

E

E

M

M

L

L

Y

T

P

P

T

T

S

S

Y

A

L

I

K

M

S

G

K

A

G

G

L

L

G

G

K

K

A

E

C

C

A

A

L

L

L

L

T

T

D

D

G

G

R

R

F

F

S

S

G

G

G

G

T

S

S

H

G

G

L

F

S

V

I

V

G

G

H

H

A

I

S

C

P

P

E

E

A

A

A

Q

A

E

G

G

G

G

A

P

I

I

G

G

L

L

V

I

Q

K

D

N

G

G

D

D

K

I

V

I

L

T

I

I

D

D

I

I

P

G

N

A

R

A

S

R

I

I

N

D

L

T

L

Q

I

V

S

S

D

P

E

E

E

E

L

M

A

N

A

D

R

R

R

R

V

-

E

-

Q

-

D

-

K

K

K

K

G

W

W

T

K

A

P

P

V

A

E

Y

K

K

R

V

P

N

R

R

K

G

V

V

T

-

T

-

A

-

L

L

K

Y

A

K

Y

Y

A

I

L

K

L

N

A

V

T

Q

S

S

A

A

D

S

K

D

G

G

A

C

V

V

R

T

N

D

K

E

binding fe2/s2 (inorganic) cluster: C62 (≠ G49), D94 (= D81), A95 (≠ G82), C135 (= C122), D136 (= D123), C207 (= C193)
binding magnesium ion: D136 (= D123), K137 (= K124), N285 (= N278), H289 (= H282)

8imuA Dihydroxyacid dehydratase (dhad) mutant-v497f
35% identity, 99% coverage: 4:607/613 of query aligns to 15:523/523 of 8imuA

query
sites
8imuA

Y

Y

R

S

S

S

K

R

T

I

S

-

T

T

H

E

G

P

R

K

N

S

M

Q

A

G

G

G

A

S

R

Q

A

A

L

I

W

L

R

H

A

G

T

V

G

G

M

L

K

S

D

D

D

D

D

D

F

L

K

L

K

K

P

P

I

Q

I

I

A

G

I

I

A

S

N

S

S

V

F

W

T

Y

Q

E

F

G

V
x
N

P

T

G
x
C

H

N

V

M

H

H

L

L

K

L

D

K

L

L

G

S

Q

E

L

A

V

V

A

K

R

E

E

G

I

V

E

E

R

N

A

A

G

G

G

M

V

V

A

G

K

F

E

R

F

F

N

N

T

T

I

I

A

G

V

V

D

S

D

D

G

A

I

I

A

S

M

M

G

G

H

T

D

R

G

G

M

M

L

C

Y

F

S

S

L

L

P

Q

S

S

R

R

E

D

I

L

I

I

A

A

D

D

S

S

V

I

E

E

Y

T

M

V

V

M

N

S

A

A

H

Q

C

W

A

Y

D

D

A

G

I

N

V

I

C

S

I

I

S

P

N

G

C
|
C

D
|
D

K

K

I

N

T

M

P

P

G

G

M

T

L

I

M

M

A

A

A

M

L

G

R

R

L

L

N

N

I

R

P

P

V

G

I

I

F

M

V

V

S

Y

G

G

G

G

P

T

M

I

E

K

A

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G

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-

T

-

K

-

L

-

A

-

S

-

H

-

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L

-

D

-

L

-

V

-

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-

M

-

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A

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-

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G

M

M

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Y

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A

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N

S

T

M

M

N

A

C

S

L

A

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I

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A

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N

A

A

M

M

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V

L

S

D

V

I

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A

A

M

L

G

G

G

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S

S

T

T

N

N

T

A

I

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L

L

H

H

L

L

L

I

A

A

A

I

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M

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-

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M

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L

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P

T

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S

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A

L

I

K

M

S

G

K

A

G

G

L

L

G

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K

K

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C

A

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L

L

L

L

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T

D

D

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G

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R

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F

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G

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A

A

A

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A

E

G

G

G

G

A

P

I

I

G

G

L

L

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I

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D

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G

G

D

D

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M

A

N

A

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R

R

R

R

V

-

E

-

Q

-

D

-

K

K

K

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W

W

T

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A

P

P

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A

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Y

K

K

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N

R

R

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G

V

V

T

-

T

-

A

-

L

L

K

Y

A

K

Y

Y

A

I

L

K

L

N

A

V

T

Q

S

S

A

A

D

S

K

D

G

G

A

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V

V

R

T

N

D

K

E

binding fe2/s2 (inorganic) cluster: N57 (≠ V47), C59 (≠ G49), C132 (= C122), D133 (= D123)

B5ZZ34 L-arabinonate dehydratase; ArDHT; D-fuconate dehydratase; Galactonate dehydratase; L-arabonate dehydratase; EC 4.2.1.25; EC 4.2.1.67; EC 4.2.1.6 from Rhizobium leguminosarum bv. trifolii (strain WSM2304) (see 2 papers)
32% identity, 96% coverage: 13:603/613 of query aligns to 22:557/579 of B5ZZ34

query
sites
B5ZZ34

R

R

N

D

M

V

A

I

G

Y

A

H

R

R

A

G

L

W

W

L

R

K

A

N

T

Q

G

G

M

Y

K

P

D

H

D

D

D

L

F

F

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-

G

K

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P

P

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I

I

A

G

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I

A

L

N

N

S

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W

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P

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x
C

H

N

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H

L

L

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L

L

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L

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V

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G

G

G

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A

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L

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P

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A

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D
x
E

G

N

I

-

A

T

M

F

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R

H

P

D

T

G

A

M

M

L

M

Y

Y

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-

L

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V

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A

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A

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D

D

A

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C

V

V

C

L

I

L

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N

G

C
|
C

D
|
D

K

K

I

T

T

T

P

P

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S

M

L

L

L

M

M

A

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M

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G

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|
C

S

N

G

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M

F

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A

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S

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M

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G

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N

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x
C

I

I

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M

I

V

I

M

R

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Y

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C

G

G

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P

K

K

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G

G

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P

G

G

M

M

Q

A

E
|
E

-

V

-

G

-

N

M

M

L

G

Y

L

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P

T

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K

Y

V

L

L

K

K

S

-

K

K

G

G

L

I

G

L

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A

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V

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L

I

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D

D

G

A

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R

F

M

S
|
S

G

G

G

T

T

A

S

Y

G

G

L

T

S

V

I

V

G

L

H

H

A

T

S

S

P

P

E

E

A

A

A

A

A

V

G

G

G

G

A

P

I

L

G

A

L

V

V

V

Q

K

D

N

G

G

D

D

K

M

V

I

L

E

I

L

D

D

I

V

P

P

N

N

R

R

S

R

I

L

N

H

L

L

L

D

I

I

S

S

D

D

E

E

E

E

L

L

A

A

A

R

R

R

R

L

V

A

E

E

Q

-

D

-

K

-

K

-

G

-

W

W

K

Q

P

P

V

N

E

H

K

D

R

L

P

P

R

T

K

-

V

-

T

-

T

-

A

-

L

-

K

S

A

G

Y

Y

A

A

L

F

L

L

A

H

T

Q

S

Q

A

H

D

V

K

E

G

G

A

A

C59 (≠ G49) binding [2Fe-2S] cluster; mutation to S: Loss of activity. Does not bind iron-sulfur cluster.
E91 (≠ D81) binding Mg(2+)
C127 (= C122) binding [2Fe-2S] cluster; mutation to S: Loss of activity. Does not bind iron-sulfur cluster.
D128 (= D123) binding Mg(2+)
C200 (= C193) binding [2Fe-2S] cluster; mutation to S: Almost loss of activity. Does not bind iron-sulfur cluster.
C436 (vs. gap) mutation to S: No change in activity. Does not affect binding of iron-sulfur cluster.
C443 (≠ E479) mutation to S: Slight decrease in activity. Does not affect binding of iron-sulfur cluster.
E453 (= E489) binding Mg(2+)
S480 (= S515) mutation to A: Loss of activity.

9evvD His579leu variant of l-arabinonate dehydratase co-crystallized with 2- oxobutyrate (see paper)
32% identity, 96% coverage: 13:603/613 of query aligns to 18:553/575 of 9evvD

query
sites
9evvD

R

R

N

D

M

V

A

I

G

Y

A

H

R

R

A

G

L

W

W

L

R

K

A

N

T

Q

G

G

M

Y

K

P

D

H

D

D

D

L

F

F

K

D

-

G

K

R

P

P

I

V

I

I

A

G

I

I

A

L

N

N

S

T

F

W

T

S

Q

D

F

M

V

T

P

P

G
x
C

H

N

V

G

H

H

L

L

K

R

D

E

L

L

G

A

Q

E

L

K

V

V

A

K

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A

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G

I

V

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W

R

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A

A

G

G

G

G

V

F

A

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K

L

E

E

F

V

N

P

T

V

I

F

A

S

V

A

D

S

D
x
E

G

N

I

-

A

T

M

F

G

R

H

P

D

T

G

A

M

M

L

M

Y

Y

S

-

L

-

P

-

S

-

R

R

E

N

I

L

I

A

A

A

D

L

S

A

V

V

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Y

E

M

A

V

I

N

R

A

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H

Q

C

P

A

M

D

D

A

G

I

C

V

V

C

L

I

L

S

V

N

G

C
|
C

D
|
D

K
|
K

I

T

T

T

P

P

G

S

M

L

L

L

M

M

A

G

A

A

L

A

R

S

L

C

N

D

I

L

P

P

V

S

I

I

F

V

V

V

S

T

G

G

G

G

P

P

M

M

E

L

A

N

G

G

-

Y

-

F

K

R

T

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K

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H

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G

G

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A

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A

L

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Y

A

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R

A

S

S

A

M

C

S

P

R

T

S

C

S

G

G

S
x
T

C
|
C

S

N

G

T

M

M

F

G

T
|
T

A

A

N

S

S

T

M

M

N

A

C

S

L

M

V

A

E

E

A

A

L

L

G

G

L

M

A

A

L

L

P

S

G

G

N

N

G

A

S

A

T

I

L

P

A

G

T

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binding 2-ketobutyric acid: E87 (≠ D81), D124 (= D123), T201 (= T198), E449 (= E489), S476 (= S515), G477 (= G516)
binding fe2/s2 (inorganic) cluster: C55 (≠ G49), C123 (= C122), D124 (= D123), T195 (≠ S192), C196 (= C193)
binding magnesium ion: E87 (≠ D81), D124 (= D123), K125 (= K124), E449 (= E489)

9evvA His579leu variant of l-arabinonate dehydratase co-crystallized with 2- oxobutyrate (see paper)
32% identity, 96% coverage: 13:603/613 of query aligns to 18:553/575 of 9evvA

query
sites
9evvA

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