SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF543 FitnessBrowser__Marino:GFF543 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P09342 Acetolactate synthase 1, chloroplastic; ALS I; Acetohydroxy-acid synthase I; Acetolactate synthase I; EC 2.2.1.6 from Nicotiana tabacum (Common tobacco) (see 2 papers)
42% identity, 98% coverage: 6:564/572 of query aligns to 96:657/667 of P09342

query
sites
P09342

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C161 (≠ V71) modified: Disulfide link with 307
P194 (≠ A104) mutation to Q: In C3; highly resistant to sulfonylurea herbicides.
C307 (≠ V217) modified: Disulfide link with 161

P09114 Acetolactate synthase 2, chloroplastic; ALS II; Acetohydroxy-acid synthase II; Acetolactate synthase II; EC 2.2.1.6 from Nicotiana tabacum (Common tobacco) (see paper)
42% identity, 98% coverage: 6:564/572 of query aligns to 93:654/664 of P09114

query
sites
P09114

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P191 (≠ A104) mutation to A: In S4-Hra; highly resistant to sulfonylurea herbicides; when associated with L-568.
W568 (= W484) mutation to L: In S4-Hra; highly resistant to sulfonylurea herbicides; when associated with A-191.

P17597 Acetolactate synthase, chloroplastic; AtALS; Acetohydroxy-acid synthase; Protein CHLORSULFURON RESISTANT 1; EC 2.2.1.6 from Arabidopsis thaliana (Mouse-ear cress) (see 8 papers)
42% identity, 99% coverage: 6:572/572 of query aligns to 99:668/670 of P17597

query
sites
P17597

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L

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A122 (= A29) mutation to V: Reduced catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.
M124 (≠ L31) mutation to E: Reduced catalytic activity. Resistant to imidazolinone herbicides and reduced sensitivity to sulfonylurea herbicides.; mutation to I: No effect on catalytic activity. Increased resistance to imidazolinone herbicides.
E144 (= E51) binding thiamine diphosphate
S186 (= S93) binding FAD
P197 (≠ A104) mutation to S: In csr1-1/GH50; resistant to sulfonylurea but not to imidazolinone herbicides.
R199 (≠ T106) mutation R->A,E: No effect on catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.
Q207 (= Q114) binding thiamine diphosphate
K220 (= K127) binding (R)-imazaquin
R246 (= R153) binding (R)-imazaquin; binding FAD
K256 (= K163) binding chlorimuron-ethyl
G308 (= G215) binding FAD
TL 331:332 (= TL 240:241) binding FAD
C340 (≠ A249) modified: Cysteine sulfinic acid (-SO2H)
LGMH 349:352 (= LGMH 258:261) binding FAD
GVRFD 371:375 (≠ GARFD 280:284) binding FAD
DR 376:377 (= DR 285:286) binding chlorimuron-ethyl
DI 395:396 (= DI 304:305) binding FAD
DV 414:415 (≠ PV 323:324) binding FAD
QH 487:488 (= QH 397:398) binding thiamine diphosphate
GG 508:509 (= GG 418:419) binding FAD
GAM 511:513 (≠ GTM 421:423) binding thiamine diphosphate
D538 (≠ E448) binding Mg(2+)
DGS 538:540 (≠ EGS 448:450) binding thiamine diphosphate
N565 (= N475) binding Mg(2+)
NQHLGM 565:570 (≠ NQALGM 475:480) binding thiamine diphosphate
H567 (≠ A477) binding Mg(2+)
W574 (= W484) binding chlorimuron-ethyl; mutation to L: Increased catalytic activity. Resistant to imidazolinone and sulfonylurea herbicides.; mutation to S: Slightly decreased catalytic activity. Resistant to imidazolinone and sulfonylurea herbicides.
S653 (≠ A557) binding chlorimuron-ethyl; mutation to A: No effect on catalytic activity or sensitivity to herbicides.; mutation to F: No effect on catalytic activity. Resistant to imidazolinone herbicides and also slightly sulfonylurea-resistant.; mutation to N: In csr1-2/GH90; no effect on catalytic activity. Resistant to imidazolinone but not to sulfonylurea herbicides.; mutation to T: No effect on catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.

7tzzA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase p197t mutant in complex with bispyribac-sodium (see paper)
42% identity, 98% coverage: 6:564/572 of query aligns to 14:575/582 of 7tzzA

query
sites
7tzzA

G

G

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A

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V

binding 2,6-bis[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid: M266 (= M260), R292 (= R286), W489 (= W484), S568 (≠ A557)
binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: V400 (= V395), G401 (= G396), Q402 (= Q397), H403 (= H398), G426 (= G421), M428 (= M423), G452 (= G447), D453 (≠ E448), G454 (= G449), S455 (= S450), L483 (= L478), G484 (= G479), M485 (= M480), V486 (= V481)
binding flavin-adenine dinucleotide: R161 (= R153), G222 (= G214), G223 (= G215), G224 (= G216), T246 (= T240), L247 (= L241), M248 (= M242), M263 (≠ W257), L264 (= L258), M266 (= M260), H267 (= H261), G286 (= G280), R288 (= R282), V293 (= V287), D310 (= D304), I311 (= I305), D329 (≠ P323), V330 (= V324), M405 (= M400), G423 (= G418)
binding magnesium ion: A37 (= A29), T82 (= T74), S83 (= S75), Q122 (= Q114), Y381 (≠ E376), D453 (≠ E448), M458 (= M453), Q461 (= Q456), N480 (= N475), H482 (≠ A477), K533 (= K521)

8et4A Crystal structure of wild-type arabidopsis thaliana acetohydroxyacid synthase in complex with amidosulfuron (see paper)
42% identity, 98% coverage: 6:564/572 of query aligns to 14:575/582 of 8et4A

query
sites
8et4A

G

G

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E

K

E

A

A

Y

F

I

L

A

A

A

T

T

S

G

G

R
|
R

P

P

G

G

P

P

V

V

V

L

V

V

D

D

I

V

P

P

K

K

D

D

-

I

-

Q

M

L

T

A

T

I

P

P

N

N

E

W

R

E

Y

Q

E

A

Y

M

A

R

Y

L

P

P

K

G

K

Y

V

M

K

S

L

-

R

R

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Y

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N

K

P

P

A

P

I

E

R

D

G

S

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H

A

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E

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Q

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-

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-

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L

M

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A

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A

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K

K

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K

P

P

I

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I

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Y

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A

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G

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C

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-

G

-

K

N

A

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E

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R

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F

V

V

R

E

L

L

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T

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G

Y

I

P

P

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T

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L

M
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M

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Y

Y

P

P

A

C

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D

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G

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W

M

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L

G

G

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M

H
|
H

G

G

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T

Y

V

E

Y

A

A

N

N

M

Y

A

A

M

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E

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H

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S

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D

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L

I

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x
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I
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I

D

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A

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I

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G

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K

T

N

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K

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x
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V

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E

G

M

M

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V

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N

A

R

N

A

E

E

K

E

P

L

D

K

A

L

D

D

A

-

I

F

A

G

S

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W

W

W

R

K

N

Q

E

I

L

D

N

E

V

W

Q

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K

A

Q

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K

H

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x
F

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K

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T

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F

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G

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A

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Q

E

Y

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A

I

I

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K

M

V

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L

C

D

R

E

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N

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A

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G
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Q

H
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H

Q

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M
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M

F

W

A

A

Q

Q

Y

F

Y

Y

K

N

F

Y

D

K

K

K

P

P

N

R

R

Q

W

W

I

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N

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G

G

G

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M

G

G

F

F

G

G

L

L

P

P

A

A

M

I

G

G

V

A

K

S

L

V

T

A

H

N

P

P

D

D

D

A

E

I

V

V

L

V

C

D

I

I

T

D

G
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G

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x
D

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G

S
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S

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M

N

N

I

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Q
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Q

E

E

L

L

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K

R

Q

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Y

E

N

N

L

L

P

P

V

V

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K

I

V

I

L

N

L

L

L

N

N

N
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N

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A
x
H

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L

G
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G

M
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M

V
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V

K

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Q

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W
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W

Q

E

D

D

M

R

N

F

Y

Y

E

K

S

A

R

N

H

R

A

A

E

H

S

T

Y

F

M

L

-

G

-

D

-

P

-

A

-

Q

-

E

-

D

E

E

S

I

L

F

P

P

D

N

F

M

I

L

K

L

L

F

A

A

E

A

A

A

Y

C

G

G

H

I

K

P

G

A

V

A

R

R

I

V

E

T

K

K

K
|
K

E

A

D

D

L

L

E

R

T

E

K

A

L

I

K

Q

E

T

V

M

L

L

A

D

M

T

K

P

D

G

E

P

L

Y

V

L

F

-

V

L

D

D

I

V

Y

I

V

C

D

P

R

H

F

Q

E

E

H

H

V

V

Y

L

P

P

M

M

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I

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A
x
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G

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binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: V400 (= V395), G401 (= G396), Q402 (= Q397), H403 (= H398), G426 (= G421), M428 (= M423), G452 (= G447), D453 (≠ E448), G454 (= G449), S455 (= S450), M458 (= M453), N480 (= N475), H482 (≠ A477), L483 (= L478), G484 (= G479), M485 (= M480), V486 (= V481)
binding flavin-adenine dinucleotide: R161 (= R153), G222 (= G214), G223 (= G215), G224 (= G216), T246 (= T240), L247 (= L241), M248 (= M242), L264 (= L258), M266 (= M260), H267 (= H261), G286 (= G280), V287 (≠ A281), R288 (= R282), D290 (= D284), R292 (= R286), V293 (= V287), D310 (= D304), I311 (= I305), D329 (≠ P323), V330 (= V324), M405 (= M400), G423 (= G418)
binding magnesium ion: F370 (≠ Y365), D453 (≠ E448), M458 (= M453), Q461 (= Q456), N480 (= N475), H482 (≠ A477), K533 (= K521)
binding N-{[(4,6-dimethoxypyrimidin-2-yl)carbamoyl]sulfamoyl}-N-methylmethanesulfonamide: M266 (= M260), R292 (= R286), M485 (= M480), W489 (= W484), S568 (≠ A557)

5wj1A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a triazolopyrimidine herbicide, penoxsulam (see paper)
42% identity, 98% coverage: 6:564/572 of query aligns to 14:575/582 of 5wj1A

query
sites
5wj1A

G

G

A

A

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K

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M

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M

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A

L

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I

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A

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L

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A

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W

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E

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E

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N

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L

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K

I

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I

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L

L

L

N

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N
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x
H

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M

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V

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W

Q

E

D

D

M

R

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F

Y

Y

E

K

S

A

R

N

H

R

A

A

E

H

S

T

Y

F

M

L

-

G

-

D

-

P

-

A

-

Q

-

E

-

D

E

E

S

I

L

F

P

P

D

N

F

M

I

L

K

L

L

F

A

A

E

A

A

A

Y

C

G

G

H

I

K

P

G

A

V

A

R

R

I

V

E

T

K

K

E

A

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D

L

L

E

R

T

E

K

A

L

I

K

Q

E

T

V

M

L

L

A

D

M

T

K

P

D

G

E

P

L

Y

V

L

F

-

V

L

D

D

I

V

Y

I

V

C

D

P

R
x
H

F

Q

E

E

H

H

V

V

Y

L

P

P

M

M

Q

I

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P

A
x
S

R

G

G

G

S

T

M

F

K

N

D

D

M

V

active site: Y33 (= Y25), G35 (= G27), G36 (= G28), A37 (= A29), S38 (≠ A30), E59 (= E51), T82 (= T74), F121 (= F113), Q122 (= Q114), E123 (= E115), K171 (= K163), M266 (= M260), V293 (= V287), V400 (= V395), G426 (= G421), M428 (= M423), D453 (≠ E448), N480 (= N475), H482 (≠ A477), L483 (= L478), M485 (= M480), V486 (= V481), W489 (= W484), H558 (≠ R547)
binding flavin-adenine dinucleotide: R161 (= R153), G222 (= G214), G223 (= G215), G224 (= G216), T246 (= T240), L247 (= L241), M248 (= M242), M263 (≠ W257), L264 (= L258), G286 (= G280), R288 (= R282), V293 (= V287), D310 (= D304), I311 (= I305), D329 (≠ P323), V330 (= V324), M405 (= M400), G423 (= G418), G424 (= G419)
binding magnesium ion: D453 (≠ E448), N480 (= N475), H482 (≠ A477)
binding 2-(2,2-difluoroethoxy)-N-(5,8-dimethoxy[1,2,4]triazolo[1,5-c]pyrimidin-2-yl)-6-(trifluoromethyl)benzenesulfonamide: M266 (= M260), D291 (= D285), R292 (= R286), M485 (= M480), W489 (= W484), S568 (≠ A557)
binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (= V395), G401 (= G396), Q402 (= Q397), H403 (= H398), M428 (= M423), D453 (≠ E448), G454 (= G449), S455 (= S450), M458 (= M453), N480 (= N475), H482 (≠ A477), L483 (= L478), G484 (= G479), M485 (= M480), V486 (= V481)

5k6tA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylamino-carbonyl-triazolinone herbicide, propoxycarbazone-sodium (see paper)
42% identity, 98% coverage: 6:564/572 of query aligns to 14:575/582 of 5k6tA

query
sites
5k6tA

G

G

A

A

D

D

M

I

L

L

I

V

R

E

S

A

L

L

Q

E

D

R

E

Q

G

G

I

V

E

E

Y

T

I

V

Y

F

G

A

Y
|
Y

P

P

G
|
G

A
|
A

A
x
S

L

M

H

E

I

I

Y

H

D

Q

A

A

L

L

F

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R

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S

K

S

V

I

K

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H

N

I

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R

R

H

H

E
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E

Q

Q

A

G

V

V

H

F

M

A

A

A

D

E

G

G

Y

Y

A

A

R

R

A

S

T

S

G

G

K

K

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P

G

G

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C

L

I

V

A

T
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T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

T

L

I

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G

G

I

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A

A

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A

A

F

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M

L

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M

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A

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M

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G

E

V

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K

H

H

N

N

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E

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I

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active site: Y33 (= Y25), G35 (= G27), G36 (= G28), A37 (= A29), S38 (≠ A30), E59 (= E51), T82 (= T74), F121 (= F113), Q122 (= Q114), E123 (= E115), K171 (= K163), M266 (= M260), V293 (= V287), V400 (= V395), G426 (= G421), M428 (= M423), D453 (≠ E448), N480 (= N475), H482 (≠ A477), L483 (= L478), M485 (= M480), V486 (= V481), W489 (= W484), H558 (≠ R547)
binding methyl 2-[(4-methyl-5-oxidanylidene-3-propoxy-1,2,4-triazol-1-yl)carbonylsulfamoyl]benzoate: H267 (= H261), R292 (= R286), M485 (= M480), W489 (= W484), S568 (≠ A557)
binding flavin-adenine dinucleotide: R161 (= R153), G222 (= G214), G223 (= G215), G224 (= G216), T246 (= T240), L247 (= L241), M248 (= M242), L264 (= L258), G286 (= G280), R288 (= R282), D290 (= D284), R292 (= R286), V293 (= V287), D310 (= D304), I311 (= I305), D329 (≠ P323), V330 (= V324), Q404 (= Q399), M405 (= M400), G423 (= G418)
binding magnesium ion: D453 (≠ E448), N480 (= N475), H482 (≠ A477)
binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: V400 (= V395), G401 (= G396), Q402 (= Q397), H403 (= H398), G426 (= G421), M428 (= M423), G452 (= G447), G454 (= G449), S455 (= S450), N480 (= N475), H482 (≠ A477), L483 (= L478), G484 (= G479)

5k6rA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylamino-carbonyl-triazolinone herbicide, thiencarbazone-methyl (see paper)
42% identity, 98% coverage: 6:564/572 of query aligns to 14:575/582 of 5k6rA

query
sites
5k6rA

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