SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF5497 FitnessBrowser__WCS417:GFF5497 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 3 hits to proteins with known functional sites (download)

Q6LX42 Sodium/alanine symporter AgcS; Alanine permease; Alanine/glycine:cation symporter from Methanococcus maripaludis (strain S2 / LL) (see paper)
35% identity, 91% coverage: 1:436/481 of query aligns to 6:446/453 of Q6LX42

query
sites
Q6LX42

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T75 (≠ R68) binding ; binding
G79 (= G72) binding
N80 (= N73) binding ; mutation to A: Markedly reduces L-alanine uptake; when associated with A-274 and A-308.
I165 (vs. gap) mutation to A: Shows poor substrate selectivity, allowing additional amino acids such as L-valine and L-leucine to be efficiently transported. Decreases L-alanine uptake.
Q170 (= Q159) binding ; binding
F273 (= F262) mutation to A: Shows poor substrate selectivity, allowing additional amino acids such as L-valine and L-leucine to be efficiently transported. Decreases L-alanine uptake.
FS 273:274 (≠ FA 262:263) binding
FSNE 273:276 (≠ FANE 262:265) binding
S274 (≠ A263) mutation to A: Markedly reduces L-alanine uptake; when associated with A-80 and A-308.
D308 (= D297) mutation to A: Markedly reduces L-alanine uptake; when associated with A-80 and A-274.
W370 (≠ N358) mutation to Q: Does not change uptake of L-alanine, but reduces the ability to transport D-alanine.

6csfM Crystal structure of sodium/alanine symporter agcs with d-alanine bound (see paper)
36% identity, 89% coverage: 11:436/481 of query aligns to 2:426/431 of 6csfM

query
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6csfM

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binding d-alanine: T58 (≠ R68), G60 (= G70), G62 (= G72), N63 (= N73), Q150 (= Q159), F253 (= F262), S254 (≠ A263)
binding sodium ion: T58 (≠ R68), I59 (≠ V69), N63 (= N73), S254 (≠ A263), D288 (= D297)

6cseM Crystal structure of sodium/alanine symporter agcs with l-alanine bound (see paper)
36% identity, 89% coverage: 11:436/481 of query aligns to 2:426/431 of 6cseM

query
sites
6cseM

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S

K

N

C

L

F

R

E

F

Y

L

L

V

I

G

G

N

T

N

K

R

G

K

I

V

R

L

L

M

-

G

-

Y

Y

R

R

A

I

L

A

V

F

L

V

V

L

L

V

I

A

F

F

W

W

G

G

S

A

I

T

E

A

N

S

L

L

S

P

T

L

V

V

F

W

A

N

F

I

A

A

D

D

I

T

T

L

M

N

T

G

M

A

L

M

A

A

F

I

V

P

N

N

L

L

F

I

A

G

L

L

A

L

F

L

L

L

F

S

K

G

I

V

A

V

M

V

R

S

I

E

L

T

N

K

D

A

Y

F

D

N

N

E

Q

I

R

R

binding alanine: A57 (≠ G67), T58 (≠ R68), G60 (= G70), N63 (= N73), Q150 (= Q159), F253 (= F262), S254 (≠ A263), E256 (= E265)
binding sodium ion: T58 (≠ R68), I59 (≠ V69), N63 (= N73), S254 (≠ A263), D288 (= D297)

Query Sequence

>GFF5497 FitnessBrowser__WCS417:GFF5497
MLEVINDFLSGKVLIVLIVGLGGYFTIRSRFVQLRHFFHMFSVFKDSLKSSAGQLSSFQA
LMLSLAGRVGAGNIAGVGIAVTLGGPGAVFWMWVTALVGMSSSFIECSLGQLYKRTDAEG
TYRGGPAYYIQHGLQKRWLGMVMAFLLLVTFGFAFNGLQAHAVTHSLNNAFGLDTTYTGL
ALAVLLGLVFIGGIKRIASIADLLVPVKTLVYIAVTLYVIVLQFDHVPAMLATIVKSAFG
LDQAFGGLVGSAIIMGVKRGVFANEAGLGSAPNVAAVASVEHPIAQGVVQAFSVFLDTFV
ICTCTALLILLSGFYTPGFEGDGIALTQNSLAAVVGDWGRMFISVALALFVFTSIMYNYY
LGESNLRFLVGNNRKVLMGYRALVLVLIFWGSIENLSTVFAFADITMTMLAFVNLFALAF
LFKIAMRILNDYDNQRAAGIKTPVFDSSQFPDLDLDRKAWPANPVKPEAATSAELNAQVQ
R

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory