SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF6 FitnessBrowser__psRCH2:GFF6 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4ykgA Crystal structure of the alkylhydroperoxide reductase subunit f (ahpf) with NAD+ from escherichia coli (see paper)
66% identity, 99% coverage: 1:516/520 of query aligns to 1:518/521 of 4ykgA

query
sites
4ykgA

M

M

L

L

D

D

T

T

N

N

L

M

K

K

T

T

Q

Q

L

L

K

K

A

A

Y

Y

L

L

E

E

K

K

V

L

T

T

Q

K

P

P

F

V

E

E

I

L

V

I

A

A

S

T

L

L

D

D

G

S

E

A

K

K

S

S

R

A

E

E

M

I

L

K

S

E

L

L

Q

A

D

E

I

I

A

A

G

E

L

L

S

S

D

D

K

K

I

V

T

T

L

F

K

K

T

E

D

D

G

N

D

S

-

L

D

P

A

V

R

R

R

K

P

P

S

S

F

F

S

L

L

I

N

T

R

N

I

P

G

G

G

S

N

N

I

Q

S

G

L

P

R

R

F

F

A

A

G

G

I

S

P

P

M

L

G

G

H

H

E

E

F

F

T

T

S

S

L

L

V

V

L

L

A

A

L

L

Q

W

V

T

G

G

H

H

P

P

S

S

K

K

T

E

A

A

P

Q

E

S

V

L

I

L

E

E

Q

Q

I

I

K

R

A

H

L

I

E

D

G

G

D

D

Y

F

R

E

F

F

E

E

T

T

Y

Y

F

Y

S

S

L

L

S

S

C

C

Q

H

N

N

C

C

P

P

D

D

V

V

Q

Q

A

A

L

L

N

N

L

L

M

M

A

S

V

V

L

L

N

N

P

P

N

R

I

I

Q

K

H

H

V

T

A

A

I

I

D

D

G

G

A

G

L

T

F

F

Q

Q

D

N

E

E

V

I

E

T

A

D

R

R

Q

N

I

V

M

M

S

G

V

V

P

P

S

A

I

V

Y

F

L

V

N

N

G

G

E

K

L

E

F

F

T

G

Q

Q

G

G

R

R

M

M

S

T

E

L

E

T

E

E

I

I

L

V

A

A

K

K

L

I

D

D

T

T

G

G

S

A

S

E

A

K

R

R

D

A

A

A

E

E

K

E

L

L

K

N

A

K

K

R

D

D

A

A

Y

Y

D

D

V

V

L

L

V

I

V

V

G

G

G

S

G
|
G

P
|
P

A
|
A

G

G

A

A

I

I

Y

Y

A

S

A

A

R

R

K

K

G

G

I

I

R

R

T

T

G

G

V

L

A

M

A
x
G

E
|
E

R

R

F

F

G

G

G
|
G

Q
|
Q

V

I

L

L

D

D

T
|
T

M

V

A

D

I

I

E

E

N
|
N

F

Y

I

I

S

S

V

V

K

P

E

K

T

T

E

E

G

G

P

Q

K

K

L

L

A

A

R

G

A

A

L

L

E

K

E

V

H

H

V

V

R

D

E

E

Y

Y

D

D

V

V

D

D

I

V

M

I

N

D

L

S

Q

Q

R
x
S

A
|
A

S

S

Q

K

L

L

I

I

P

P

A

A

G

A

-

V

D

E

D

G

G

G

L

L

H

H

R

Q

V

I

Q

E

F

T

E

A

N

S

G

G

G

A

E

V

L

L

K

K

A

A

K

R

T

S

L

I

I

I

L

V

A

A

T
|
T

G
|
G

A

A

R

K

W

W

R

R

E

N

M

M

N

N

V

V

P

P

G

G

E

E

Q

D

E

Q

Y

Y

R

R

G

T

R

K

G

G

V

V

A

T

Y

Y

C
|
C

P

P

H

H

C
|
C

D
|
D

G

G

P

P

L

L

F

F

K

K

G

G

K

K

R

R

V

V

A

A

V

V

I
|
I

G

G

G
|
G

N

N

S
|
S

G

G

V

V

E

E

A

A

I

I

D

D

L

L

A

A

G

G

I

I

V

V

A

E

H

H

V

V

T

T

L

L

E
|
E

F
|
F

G

A

E

P

E

E

L

M

R

K

A

A

D

D

A

Q

V

V

L

L

Q

Q

R

D

K

K

L

L

F

R

S

S

L

L

P

K

N

N

V

V

R

D

V

I

L

I

K

L

M

N

A

A

Q

Q

T

T

E

E

V

V

K

K

G

G

D

D

G

G

Q

S

K

K

V

V

T

V

G

G

L

L

V

E

Y

Y

K

R

D

D

R

R

T

V

N

S

D

G

E

D

V

I

H

H

S

N

V

I

E

E

L

L

E

A

G

G

I

I

F

F

V

V

Q

Q

I
|
I

G

G

L

L

P

P

N

N

S

T

E

N

W

W

L

L

K

E

G

G

T

A

L

V

E

E

L

R

S

N

R

R

F
x
M

G

G

D

E

I

I

V

I

D

D

A

A

K

K

G

C

Q

E

T

T

S

N

L

V

P

K

G

G

V

V

F

F

A

A

G
|
G

D
|
D

V

C

T

T

V

V

P
|
P

Y

Y

K

K

Q
|
Q

I
|
I

V

I

I

I

A

A

V

T

G

G

E

E

G

G

A

A

K

K

A

A

S

S

L

L

S

S

A

A

F

F

D

D

H

Y

L

L

I

I

R

R

S

T

active site: C345 (= C343), C348 (= C346), D349 (= D347)
binding flavin-adenine dinucleotide: G221 (= G220), P222 (= P221), A223 (= A222), G242 (≠ A241), E243 (= E242), G247 (= G246), Q248 (= Q247), T252 (= T251), N257 (= N256), S289 (≠ R288), A290 (= A289), T322 (= T320), G323 (= G321), C348 (= C346), G487 (= G485), D488 (= D486), Q496 (= Q494), I497 (= I495)
binding nicotinamide-adenine-dinucleotide: I361 (= I359), G364 (= G362), S366 (= S364), E385 (= E383), F386 (= F384), I449 (= I447), M467 (≠ F465), P493 (= P491)

4ykfA Crystal structure of the alkylhydroperoxide reductase subunit f (ahpf) with nadh from escherichia coli (see paper)
66% identity, 99% coverage: 1:516/520 of query aligns to 1:518/521 of 4ykfA

query
sites
4ykfA

M

M

L

L

D

D

T

T

N

N

L

M

K

K

T

T

Q

Q

L

L

K

K

A

A

Y

Y

L

L

E

E

K

K

V

L

T

T

Q

K

P

P

F

V

E

E

I

L

V

I

A

A

S

T

L

L

D

D

G

S

E

A

K

K

S

S

R

A

E

E

M

I

L

K

S

E

L

L

Q

A

D

E

I

I

A

A

G

E

L

L

S

S

D

D

K

K

I

V

T

T

L

F

K

K

T

E

D

D

G

N

D

S

-

L

D

P

A

V

R

R

R

K

P

P

S

S

F

F

S

L

L

I

N

T

R

N

I

P

G

G

G

S

N

N

I

Q

S

G

L

P

R

R

F

F

A

A

G

G

I

S

P

P

M

L

G

G

H

H

E

E

F

F

T

T

S

S

L

L

V

V

L

L

A

A

L

L

Q

W

V

T

G

G

H

H

P

P

S

S

K

K

T

E

A

A

P

Q

E

S

V

L

I

L

E

E

Q

Q

I

I

K

R

A

H

L

I

E

D

G

G

D

D

Y

F

R

E

F

F

E

E

T

T

Y

Y

F

Y

S

S

L

L

S

S

C

C

Q

H

N

N

C

C

P

P

D

D

V

V

Q

Q

A

A

L

L

N

N

L

L

M

M

A

S

V

V

L

L

N

N

P

P

N

R

I

I

Q

K

H

H

V

T

A

A

I

I

D

D

G

G

A

G

L

T

F

F

Q

Q

D

N

E

E

V

I

E

T

A

D

R

R

Q

N

I

V

M

M

S

G

V

V

P

P

S

A

I

V

Y

F

L

V

N

N

G

G

E

K

L

E

F

F

T

G

Q

Q

G

G

R

R

M

M

S

T

E

L

E

T

E

E

I

I

L

V

A

A

K

K

L

I

D

D

T

T

G

G

S

A

S

E

A

K

R

R

D

A

A

A

E

E

K

E

L

L

K

N

A

K

K

R

D

D

A

A

Y

Y

D

D

V

V

L

L

V

I

V

V

G

G

G

S

G
|
G

P
|
P

A
|
A

G

G

A

A

I

I

Y

Y

A

S

A

A

R

R

K

K

G

G

I

I

R

R

T

T

G

G

V

L

A

M

A
x
G

E
|
E

R

R

F

F

G

G

G
|
G

Q
|
Q

V

I

L

L

D

D

T

T

M

V

A

D

I

I

E

E

N
|
N

F

Y

I

I

S

S

V

V

K

P

E

K

T

T

E

E

G

G

P

Q

K

K

L

L

A

A

R

G

A

A

L

L

E

K

E

V

H

H

V

V

R

D

E

E

Y

Y

D

D

V

V

D

D

I

V

M

I

N

D

L

S

Q

Q

R

S

A
|
A

S

S

Q

K

L

L

I

I

P

P

A

A

G

A

-

V

D

E

D

G

G

G

L

L

H

H

R

Q

V

I

Q

E

F

T

E

A

N

S

G

G

G

A

E

V

L

L

K

K

A

A

K

R

T

S

L

I

I

I

L

V

A

A

T
|
T

G
|
G

A

A

R

K

W

W

R

R

E

N

M

M

N

N

V

V

P

P

G

G

E

E

Q

D

E

Q

Y

Y

R

R

G

T

R

K

G

G

V

V

A

T

Y

Y

C
|
C

P

P

H

H

C
|
C

D
|
D

G

G

P

P

L

L

F

F

K

K

G

G

K

K

R

R

V

V

A

A

V

V

I
|
I

G

G

G
|
G

N
|
N

S
|
S

G

G

V

V

E

E

A

A

I

I

D

D

L

L

A

A

G

G

I

I

V

V

A

E

H

H

V

V

T

T

L

L

E
|
E

F
|
F

G

A

E

P

E

E

L

M

R
x
K

A

A

D

D

A

Q

V

V

L

L

Q

Q

R

D

K

K

L

L

F

R

S

S

L

L

P

K

N

N

V

V

R

D

V

I

L

I

K

L

M

N

A

A

Q

Q

T

T

E

E

V

V

K

K

G

G

D

D

G

G

Q

S

K

K

V

V

T

V

G

G

L

L

V

E

Y

Y

K

R

D

D

R

R

T

V

N

S

D

G

E

D

V

I

H

H

S

N

V

I

E

E

L

L

E

A

G

G

I

I

F

F

V

V

Q

Q

I
|
I

G

G

L

L

P

P

N
|
N

S

T

E

N

W

W

L

L

K

E

G

G

T

A

L

V

E

E

L

R

S

N

R

R

F
x
M

G

G

D

E

I

I

V

I

D

D

A

A

K

K

G

C

Q

E

T

T

S

N

L

V

P

K

G

G

V

V

F

F

A

A

G

G

D
|
D

V

C

T

T

V

V

P

P

Y

Y

K

K

Q
|
Q

I
|
I

V

I

I

I

A

A

V

T

G

G

E

E

G

G

A

A

K

K

A

A

S

S

L

L

S

S

A

A

F

F

D

D

H

Y

L

L

I

I

R

R

S

T

active site: C345 (= C343), C348 (= C346), D349 (= D347)
binding flavin-adenine dinucleotide: G221 (= G220), P222 (= P221), A223 (= A222), G242 (≠ A241), E243 (= E242), G247 (= G246), Q248 (= Q247), N257 (= N256), A290 (= A289), T322 (= T320), G323 (= G321), C348 (= C346), N454 (= N452), D488 (= D486), Q496 (= Q494), I497 (= I495)
binding 1,4-dihydronicotinamide adenine dinucleotide: I361 (= I359), G364 (= G362), N365 (= N363), S366 (= S364), E385 (= E383), F386 (= F384), K391 (≠ R389), I449 (= I447), M467 (≠ F465)

P35340 Alkyl hydroperoxide reductase subunit F; Alkyl hydroperoxide reductase F52A protein; EC 1.8.1.- from Escherichia coli (strain K12) (see paper)
66% identity, 99% coverage: 1:516/520 of query aligns to 1:518/521 of P35340

query
sites
P35340

M

M

L

L

D

D

T

T

N

N

L

M

K

K

T

T

Q

Q

L

L

K

K

A

A

Y

Y

L

L

E

E

K

K

V

L

T

T

Q

K

P

P

F

V

E

E

I

L

V

I

A

A

S

T

L

L

D

D

G

S

E

A

K

K

S

S

R

A

E

E

M

I

L

K

S

E

L

L

Q

A

D

E

I

I

A

A

G

E

L

L

S

S

D

D

K

K

I

V

T

T

L

F

K
|
K

T

E

D

D

G

N

D

S

-

L

D

P

A

V

R

R

R

K

P

P

S

S

F

F

S

L

L

I

N

T

R

N

I

P

G

G

G

S

N

N

I

Q

S

G

L

P

R

R

F

F

A

A

G

G

I

S

P

P

M

L

G

G

H

H

E

E

F

F

T

T

S

S

L

L

V

V

L

L

A

A

L

L

Q

W

V

T

G

G

H

H

P

P

S

S

K

K

T

E

A

A

P

Q

E

S

V

L

I

L

E

E

Q

Q

I

I

K

R

A

H

L

I

E

D

G

G

D

D

Y

F

R

E

F

F

E

E

T

T

Y

Y

F

Y

S

S

L

L

S

S

C

C

Q

H

N

N

C

C

P

P

D

D

V

V

Q

Q

A

A

L

L

N

N

L

L

M

M

A

S

V

V

L

L

N

N

P

P

N

R

I

I

Q

K

H

H

V

T

A

A

I

I

D

D

G

G

A

G

L

T

F

F

Q

Q

D

N

E

E

V

I

E

T

A

D

R

R

Q

N

I

V

M

M

S

G

V

V

P

P

S

A

I

V

Y

F

L

V

N

N

G

G

E

K

L

E

F

F

T

G

Q

Q

G

G

R

R

M

M

S

T

E

L

E

T

E

E

I

I

L

V

A

A

K

K

L

I

D

D

T

T

G

G

S

A

S

E

A

K

R

R

D

A

A

A

E

E

K

E

L

L

K

N

A

K

K

R

D

D

A

A

Y

Y

D

D

V

V

L

L

V

I

V

V

G

G

G

S

G

G

P

P

A

A

G

G

A

A

I

I

Y

Y

A

S

A

A

R

R

K

K

G

G

I

I

R

R

T

T

G

G

V

L

A

M

A

G

E

E

R

R

F

F

G

G

Q

Q

V

I

L

L

D

D

T

T

M

V

A

D

I

I

E

E

N

N

F

Y

I

I

S

S

V

V

K

P

E

K

T

T

E

E

G

G

P

Q

K

K

L

L

A

A

R

G

A

A

L

L

E

K

E

V

H

H

V

V

R

D

E

E

Y

Y

D

D

V

V

D

D

I

V

M

I

N

D

L

S

Q

Q

R

S

A

A

S

S

Q

K

L

L

I

I

P

P

A

A

G

A

-

V

D

E

D

G

G

G

L

L

H

H

R

Q

V

I

Q

E

F

T

E

A

N

S

G

G

G

A

E

V

L

L

K

K

A

A

K

R

T

S

L

I

I

I

L

V

A

A

T

T

G

G

A

A

R

K

W

W

R

R

E

N

M

M

N

N

V

V

P

P

G

G

E

E

Q

D

E

Q

Y

Y

R

R

G

T

R

K

G

G

V

V

A

T

Y

Y

C

C

P

P

H

H

C

C

D

D

G

G

P

P

L

L

F

F

K
|
K

G

G

K

K

R

R

V

V

A

A

V

V

I

I

G

G

N

N

S

S

G

G

V

V

E

E

A

A

I

I

D

D

L

L

A

A

G

G

I

I

V

V

A

E

H

H

V

V

T

T

L

L

E

E

F

F

G

A

E

P

E

E

L

M

R

K

A

A

D

D

A

Q

V

V

L

L

Q

Q

R

D

K

K

L

L

F

R

S

S

L

L

P

K

N

N

V

V

R

D

V

I

L

I

K

L

M

N

A

A

Q

Q

T

T

E

E

V

V

K

K

G

G

D

D

G

G

Q

S

K

K

V

V

T

V

G

G

L

L

V

E

Y

Y

K

R

D

D

R

R

T

V

N

S

D

G

E

D

V

I

H

H

S

N

V

I

E

E

L

L

E

A

G

G

I

I

F

F

V

V

Q

Q

I

I

G

G

L

L

P

P

N

N

S

T

E

N

W

W

L

L

K

E

G

G

T

A

L

V

E

E

L

R

S

N

R

R

F

M

G

G

D

E

I

I

V

I

D

D

A

A

K

K

G

C

Q

E

T

T

S

N

L

V

P

K

G

G

V

V

F

F

A

A

G

G

D

D

V

C

T

T

V

V

P

P

Y

Y

K

K

Q

Q

I

I

V

I

I

I

A

A

V

T

G

G

E

E

G

G

A

A

K

K

A

A

S

S

L

L

S

S

A

A

F

F

D

D

H

Y

L

L

I

I

R

R

S

T

K53 (= K53) modified: N6-acetyllysine
K354 (= K352) modified: N6-acetyllysine

1hyuA Crystal structure of intact ahpf (see paper)
66% identity, 99% coverage: 1:516/520 of query aligns to 1:518/521 of 1hyuA

query
sites
1hyuA

M

M

L

L

D

D

T

T

N

N

L

M

K

K

T

T

Q

Q

L

L

K

R

A

A

Y

Y

L

L

E

E

K

K

V

L

T

T

Q

K

P

P

F

V

E

E

I

L

V

I

A

A

S

T

L

L

D

D

G

S

E

A

K

K

S

S

R

A

E

E

M

I

L

K

S

E

L

L

Q

A

D

E

I

I

A

A

G

E

L

L

S

S

D

D

K

K

I

V

T

T

L

F

K

K

T

E

D

D

G

N

D

T

-

L

D

P

A

V

R

R

R

K

P

P

S

S

F

F

S

L

L

I

N

T

R

N

I

P

G

G

G

S

N

Q

I

Q

S

G

L

P

R

R

F

F

A

A

G

G

I

S

P

P

M

L

G

G

H

H

E

E

F

F

T

T

S

S

L

L

V

V

L

L

A

A

L

L

Q

W

V

T

G

G

H

H

P

P

S

S

K

K

T

E

A

A

P

Q

E

S

V

L

I

L

E

E

Q

Q

I

I

K

R

A

D

L

I

E

D

G

G

D

D

Y

F

R

E

F

F

E

E

T

T

Y

Y

F

Y

S

S

L

L

S

S

C

C

Q

H

N

N

C

C

P

P

D

D

V

V

Q

Q

A

A

L

L

N

N

L

L

M

M

A

A

V

V

L

L

N

N

P

P

N

R

I

I

Q

K

H

H

V

T

A

A

I

I

D

D

G

G

A

G

L

T

F

F

Q

Q

D

N

E

E

V

I

E

T

A

E

R

R

Q

N

I

V

M

M

S

G

V

V

P

P

S

A

I

V

Y

F

L

V

N

N

G

G

E

K

L

E

F

F

T

G

Q

Q

G

G

R

R

M

M

S

T

E

L

E

T

E

E

I

I

L

V

A

A

K

K

L

V

D

D

T

T

G

G

S

A

S

E

A

K

R

R

D

A

A

A

E

E

K

A

L

L

K

N

A

K

K

R

D

D

A

A

Y

Y

D

D

V

V

L

L

V

I

V

V

G

G

G

S

G
|
G

P
|
P

A
|
A

G

G

A

A

I

V

Y

Y

A

S

A

A

R

R

K

K

G

G

I

I

R

R

T

T

G

G

V

L

A

M

A

G

E
|
E

R

R

F

F

G

G

G
|
G

Q
|
Q

V

V

L

L

D

D

T

T

M

V

A

D

I

I

E

E

N
|
N

F

Y

I

I

S

S

V

V

K

P

E

K

T

T

E

E

G

G

P

Q

K

K

L

L

A

A

R

G

A

A

L

L

E

K

E

A

H

H

V

V

R

S

E

D

Y

Y

D

D

V

V

D

D

I

V

M

I

N

D

L

S

Q

Q

R
x
S

A
|
A

S

S

Q

K

L

L

I

V

P

P

A

A

G

A

D

T

D

E

G

G

-

G

L

L

H

H

R

Q

V

I

Q

E

F

T

E

A

N

S

G

G

G

A

E

V

L

L

K

K

A

A

K

R

T

S

L

I

I

I

L

I

A

A

T
|
T

G
|
G

A

A

R

K

W
|
W

R

R

E

N

M

M

N

N

V

V

P

P

G

G

E

E

Q

D

E

Q

Y

Y

R

R

G

T

R

K

G

G

V

V

A

T

Y

Y

C
|
C

P

P

H

H

C

C

D

D

G

G

P

P

L

L

F

F

K

K

G

G

K

K

R

R

V

V

A

A

V

V

I

I

G

G

N

N

S

S

G

G

V

V

E

E

A

A

I

I

D

D

L

L

A

A

G

G

I

I

V

V

A

E

H

H

V

V

T

T

L

L

E

E

F

F

G

A

E

P

E

E

L

M

R

K

A

A

D

D

A

Q

V

V

L

L

Q

Q

R

D

K

K

L

V

F

R

S

S

L

L

P

K

N

N

V

V

R

D

V

I

L

I

K

L

M

N

A

A

Q

Q

T

T

E

E

V

V

K

K

G

G

D

D

G

G

Q

S

K

K

V

V

T

V

G

G

L

L

V

E

Y

Y

K

R

D

D

R

R

T

V

N

S

D

G

E

D

V

I

H

H

S

S

V

V

E

A

L

L

E

A

G

G

I

I

F

F

V

V

Q

Q

I

I

G

G

L

L

P

P

N

N

S

T

E

H

W

W

L

L

K

E

G

G

T

A

L

L

E

E

L

R

S

N

R

R

F

M

G

G

D

E

I

I

V

I

D

D

A

A

K

K

G

C

Q

E

T

T

S

S

L

V

P

K

G

G

V

V

F

F

A

A

G

G

D
|
D

V

C

T

T

V

V

P

P

Y

Y

K
|
K

Q
|
Q

I
|
I

V

I

I

I

A

A

V

T

G

G

E

E

G

G

A

A

K

K

A

A

S

S

L

L

S

S

A

A

F

F

D

D

H

Y

L

L

I

I

R

R

S

T

binding flavin-adenine dinucleotide: G221 (= G220), P222 (= P221), A223 (= A222), E243 (= E242), G247 (= G246), Q248 (= Q247), N257 (= N256), S289 (≠ R288), A290 (= A289), T322 (= T320), G323 (= G321), W326 (= W324), C345 (= C343), D488 (= D486), K495 (= K493), Q496 (= Q494), I497 (= I495)

7jypA Structure of thioredoxin reductase from the thermophilic eubacterium thermosipho africanus tcf52b
39% identity, 57% coverage: 209:505/520 of query aligns to 3:296/305 of 7jypA

query
sites
7jypA

K

K

D

D

A

Y

Y

Y

D

D

V

I

L

L

V

I

G
|
G

G

G

G
|
G

P
|
P

A
|
A

G

G

A

L

A

T

A

A

I

I

Y

Y

A

A

A

G

R

R

K

A

G

G

I

L

R

S

T

A

G

A

V

V

A
x
F

A
x
E

E
x
K

R

A

F

L

-

E

G

G

G
|
G

Q
x
A

V

V

L

T

D

Q

T

T

M

H

A

V

I

V

E

E

N

N

F

W

I

P

S

G

V

F

K

I

E

R

T

I

E

E

G

G

P

S

K

E

L

L

A

G

R

E

A

K

L

F

E

A

E

E

H

H

V

A

R

K

E

A

Y

F

D

G

V

A

D

E

I

I

M

I

N

T

L

-

Q

-

R

-

A

A

S

E

Q
x
V

L

L

I

K

P

I

A

S

G

Y

D

D

D

N

G

E

L

Y

H

K

R

Y

V

V

Q

E

F

L

E

D

N

N

G

G

G

K

E

K

L

V

K

K

A

G

K

K

T

V

L

L

I

I

L

Y

A

A

T
|
T

G
|
G

A

A

R

V

W

P

R

R

E

K

M

L

N

G

V

V

P

P

G

G

E

E

Q

E

E

E

Y

F

R

R

G

G

R

R

G

G

V

V

A

T

Y

Y

C

C

P

A

H

A

C

C

D

D

G

G

P

Y

L

L

F

F

K

S

G

G

K

K

R

D

V

I

A

V

V

V

I

V

G

G

G
|
G

N

D

S
|
S

G

A

V

C

E

D

A

E

A

A

I

H

D

F

L

L

A

A

G

K

I

M

V

V

A

K

H

S

V

I

T

T

L

M

L

V

E
x
Q

F
x
N

G

L

E

P

E

Y

L

L

R
x
T

A

A

D

A

A

K

V

V

L

L

Q

Q

R

D

K

R

L

L

F

L

S

E

L

N

P

K

N

N

V

V

R

K

V

V

L

I

K

L

M

N

A

S

Q

L

T

V

T

K

E

E

V

I

K

R

G

G

D

K

G

D

Q

K

K

V

V

E

T

E

G

V

L

V

V

V

Y

V

K

N

D

N

R

E

T

T

N

G

D

E

E

E

V

T

H

-

S

V

V

I

E

K

L

A

E

E

G

G

I

V

F

F

V

I

Q

Y

I
x
V

G

G

L

L

L

V

P

P

N

K

S

S

E

D

W

L

L

L

K

K

G

G

T

I

L

V

E

D

L

I

S

N

R

E

F

Y

G

G

D

Y

I

I

I

K

V

T

D

D

A

E

K

N

G

M

Q

E

T

T

S

N

L

V

P

P

G

G

V

I

F

Y

A

A

A

V

G
|
G

D
|
D

V

V

T

R

T

E

V

K

P

N

Y

L

K

R

Q

Q

I

I

V

V

I

T

A

A

V

A

G

A

E

D

G

G

A

A

K

I

A

A

binding flavin-adenine dinucleotide: G12 (= G218), G14 (= G220), P15 (= P221), A16 (= A222), F34 (≠ A240), E35 (≠ A241), K36 (≠ E242), G41 (= G246), A42 (≠ Q247), V83 (≠ Q291), T112 (= T320), G113 (= G321), G276 (= G485), D277 (= D486)
binding nicotinamide-adenine-dinucleotide: G153 (= G361), G154 (= G362), S156 (= S364), Q175 (≠ E383), N176 (≠ F384), T181 (≠ R389), V238 (≠ I447)

3ctyB Crystal structure of t. Acidophilum thioredoxin reductase (see paper)
38% identity, 58% coverage: 212:514/520 of query aligns to 5:304/305 of 3ctyB

query
sites
3ctyB

Y

F

D

D

V

V

L

V

V

I

V
|
V

G
|
G

G

A

G

G

P

A

A
|
A

G

G

A

F

A

S

A

A

I

V

Y

Y

A

A

R

R

K

S

G

G

I

F

R

S

T

V

G

A

V

I

A

L

A
x
D

E
x
K

R

A

F

V

-
x
A

G

G

G
|
G

Q
x
L

V
x
T

L

A

D

E

T
x
A

M
x
P

A
x
L

I

V

E

E

N
|
N

F

Y

I

L

S

G

V

F

K

K

E

S

T

I

E

V

G

G

P

S

K

E

L

L

A

A

R

K

A

L

L

F

E

A

E

D

H

H

V

A

R

A

E

N

Y

Y

-

A

-

K

-

I

-

R

-

E

D

G

V

V

D

E

I
x
V

M

R

N

S

L

I

Q

K

R

K

A

T

S

Q

Q

G

-

G

-

F

L

D

I

I

P

E

A

T

G

N

D

D

G

T

L

Y

H

H

R

-

V

-

Q

-

F

-

E

-

N

-

G

-

E

-

L

-

K

-

A

A

K

K

T

Y

L

V

I

I

L

I

A

T

T
|
T

G
|
G

A

T

R

T

W

H

R

K

E

H

M

L

N

G

V

V

P

K

G

G

E

E

Q

S

E

E

Y

Y

R

F

G

G

R

K

G

G

V

T

A

S

Y

Y

C
|
C

P

S

H

T

C
|
C

D
|
D

G

G

P

Y

L

L

F

F

K

K

G

G

K

K

R

R

V

V

A

V

V

T

I

I

G

G

N

N

S
|
S

G

G

V

A

E

I

A

A

I

I

D

S

L

M

A

S

G

E

I

Y

V

V

A

K

H

N

V

V

T

T

L

I

E

E

F

Y

G

M

E

P

E

K

L

Y

R

M

A

C

D

E

A

N

V

A

L

Y

Q

V

R

Q

K

E

L

I

F

-

S

K

L

K

P

R

N

N

V

I

R

P

V

Y

L

I

K

M

M

N

A

A

Q

Q

T

V

T

T

E

E

V

I

K

V

G

G

D

D

G

G

Q

K

K

K

V

V

T

T

G

G

L

V

V

K

Y

Y

K

K

D

D

R

R

T

T

N

T

D

G

E

E

V

E

H

K

S

L

V

I

E

E

L

T

E

D

G

G

I

V

F

F

V

I

Q

Y

I

V

G

G

L

L

L

I

P

P

N
x
Q

S

T

E

S

W

F

L

L

K

K

G

D

T

S

-

G

L

V

E

K

L

L

S

D

R

E

F

R

G

G

D

Y

I

I

I

V

V

V

D

D

A

S

K

R

G

Q

Q

R

T

T

S

S

L

V

P

P

G

G

V

V

F

Y

A

A

G
|
G

D
|
D

V

V

T

T

T

S

V

G

P

N

Y

F

K
x
A

Q
|
Q

I
|
I

V

A

I

S

A

A

V

V

G

G

E

D

G

G

A

C

K

K

A

A

S

A

L

L

S

S

A

L

F

Y

D

S

H

D

L

S

I

I

active site: A38 (vs. gap), T42 (≠ V248), L47 (≠ A253), N50 (= N256), C133 (= C343), C136 (= C346), D137 (= D347)
binding flavin-adenine dinucleotide: V10 (= V217), G11 (= G218), A15 (= A222), D34 (≠ A241), K35 (≠ E242), G40 (= G246), L41 (≠ Q247), T42 (≠ V248), A45 (≠ T251), P46 (≠ M252), N50 (= N256), V82 (≠ I283), T110 (= T320), G111 (= G321), S154 (= S364), Q241 (≠ N452), G275 (= G485), D276 (= D486), A283 (≠ K493), Q284 (= Q494), I285 (= I495)

3f8pD Structure of sulfolobus solfataricus trxr-b3 (see paper)
34% identity, 59% coverage: 206:513/520 of query aligns to 1:307/310 of 3f8pD

query
sites
3f8pD

L

V

K

K

A

P

K

G

D

E

A

K

Y

F

D

D

V

V

L

I

V

I

V
|
V

G
|
G

G
x
L

G
|
G

P
|
P

A
|
A

G

A

A

Y

A

G

A

A

I

L

Y

Y

A

S

A

A

R

R

K

Y

G

M

I

L

R

K

T

T

G

L

V

V

A

I

A
x
G

E

E

R
x
T

F

P

G

G

G
|
G

Q
|
Q

V

L

L

T

D

E

T

A

M

G

A

I

I

V

E

D

N

D

F

Y

I

L

S

G

V

L

K

I

E

E

T

I

E

Q

G

A

P

S

K

D

L

M

A

I

R

K

A

V

L

F

E

N

E

K

H

H

V

I

R

E

E

K

Y

Y

D

E

V

V

D

P

I

V

M

L

N

-

L

L

Q

D

R
x
I

A
x
V

S

E

Q

K

L

I

I

E

P

N

A

R

G

G

D

D

D

E

G

F

L

V

H

-

R

-

V

V

Q

K

F

T

E

K

N

R

G

K

G

G

E

E

L

F

K

K

A

A

K

D

T

S

L

V

I

I

L

L

A
x
G

T
x
I

G
|
G

A

V

R

K

W

R

R

R

E

K

M

L

N

G

V

V

P

P

G

G

E

E

Q

Q

E

E

Y

F

R

A

G

G

R

R

G

G

V

I

A

S

Y

Y

C
|
C

P

S

H

V

C
|
C

D
|
D

G

A

P

P

L

L

F

F

K

K

G

N

K

R

R

V

V

V

A

A

V

V

I

I

G

G

N

D

S

S

G

A

V

L

E

E

A

G

A

A

I

E

D

I

L

L

A

S

G

S

I

Y

V

S

A

T

H

K

V

V

T

Y

L

L

L

I

E

H

F

R

G

R

E

D

E

T

L

F

R

K

A

A

D

Q

A

P

V

I

L

Y

Q

V

R

E

K

T

L

V

F

K

S

K

L

K

P

P

N

N

V

V

R

E

V

F

L

V

K

L

M

N

A

S

Q

V

T

V

T

K

E

E

V

I

K

K

G

G

D

D

G

-

Q

K

K

V

V

V

T

K

G

Q

L

V

V

V

Y

V

K

E

D

N

R

L

T

K

N

T

D

G

E

E

V

I

H

K

S

E

V

L

E

N

L

V

E

N

G

G

I

V

F

F

V

I

Q

E

I

I

G

G

L

F

L

D

P

P

N

P

S

T

E

D

W

F

L

A

K

K

G

S

T

N

-

G

L

I

E

E

L

T

S

D

R

T

F

N

G

G

D

Y

I

I

I

K

V

V

D

D

A

E

K

W

G

M

Q

R

T

T

S

S

L

V

P

P

G

G

V

V

F

F

A

A

G
|
G

D
|
D

V

C

T

T

T

S

-

A

-

W

V

L

P

G

Y

F

K

R

Q

Q

I

V

V

I

I

T

A

A

V

V

G

A

E

Q

G

G

A

A

K

V

A

A

S

A

L

T

S

S

A

A

F

Y

D

R

H

Y

L

V

active site: C135 (= C343), C138 (= C346), D139 (= D347)
binding nicotinamide-adenine-dinucleotide: V12 (= V217), G13 (= G218), L14 (≠ G219), G15 (= G220), P16 (= P221), A17 (= A222), G36 (≠ A241), T38 (≠ R243), G41 (= G246), Q42 (= Q247), I82 (≠ R288), V83 (≠ A289), G111 (≠ A319), I112 (≠ T320), G113 (= G321), G277 (= G485), D278 (= D486)

5mh4A Crystal structure of lactococcus lactis thioredoxin reductase (fr conformation) (see paper)
35% identity, 58% coverage: 212:514/520 of query aligns to 2:301/303 of 5mh4A

query
sites
5mh4A

Y

Y

D

D

V

V

L

V

V

I

G

G

G

S

G
|
G

P
|
P

A
|
A

G

G

A

M

A

T

A

A

I

M

Y

Y

A

T

A

A

R

R

K

S

G

E

I

M

R

K

T

T

G

-

V

L

A

L

E
|
E

R
|
R

F

G

-
x
V

-
x
P

G

G

G
|
G

Q
|
Q

V
x
M

L

N

D

N

T
|
T

M

A

A
x
E

I

I

E

E

N
|
N

F

Y

I

P

S

G

V

Y

K

E

E

T

T

I

E

M

G

G

P

P

K

E

L

L

A

S

R

M

A

K

L

M

E

A

E

E

H

-

V

-

R

-

E

-

Y

-

D

P

V

L

D

E

I

G

M

L

N

G

L

V

Q

E

R

N

A

A

S

Y

Q
x
G

L

F

I
x
V

P

T

A

G

G

I

D

E

D

D

-

H

G

G

L

D

H

Y

R

K

V

K

Q

I

F

I

E

T

N

E

G

D

E

E

L

F

K

I

A

T

K

K

T

S

L

I

I

I

L

I

A

A

T
|
T

G
|
G

A

A

R

N

W

H

R

R

E

K

M

L

N

E

V

I

P

P

G

G

E

E

Q

E

E

E

Y

Y

R

G

G

A

R

R

G

G

V

V

A

S

Y

Y

C
|
C

P

A

H

V

C
|
C

D
|
D

G

G

P

A

L

F

F

F

K

R

G

N

K

Q

R

E

V

I

A

L

V

V

I
|
I

G
|
G

N
x
D

S
|
S

G

A

V

V

E
|
E

A
x
E

A

A

I

L

D

Y

L

L

A

T

G

R

I

F

V

G

A

Q

H

S

V

V

T

T

L

I

L

M

E
x
H

F
x
R

G
x
R

E

D

E

K

L

L

R
|
R

A

A

D

Q

A

E

V

I

L

I

Q

Q

R

Q

K

R

L

A

F

F

S

K

L

E

P

E

N

K

V

I

R

N

V

F

L

I

K

W

M

D

A

S

Q

V

T

P

T

M

E

E

V

I

K

K

G

G

D

D

G

D

Q

K

K

K

V

I

T

Q

G

S

L

V

V

V

Y

Y

K

K

D

N

R

V

T

K

N

T

D

G

E

E

V

V

H

T

S

E

V

K

E

A

L

F

E

G

G

G

I

I

F

F

V

I

Q

Y

I
x
V

G
|
G

L

L

L

D

P

P

N
x
V

S

A

E

E

W
x
F

L

V

K

S

G

D

T

L

L

G

E

I

L

T

S

D

R

E

F

A

G

G

D

W

I

I

V

T

D

D

A

D

K

H

G

M

Q

R

T

T

S

N

L

I

P

P

G

G

V

I

F

F

A

A

A

V

G
|
G

D
|
D

V

V

T

R

T

Q

V

K

P

D

Y

F

K
x
R

Q
|
Q

I
|
I

V

T

I

T

A

A

V

V

G

G

E

D

G

G

A

A

K

Q

A

A

S

A

L

Q

S

E

A

A

F

Y

D

K

H

F

L

V

I

V

active site: V34 (vs. gap), P35 (vs. gap), M39 (≠ V248), E44 (≠ A253), C130 (= C343), C133 (= C346), D134 (= D347)
binding flavin-adenine dinucleotide: G10 (= G220), P11 (= P221), A12 (= A222), E31 (= E242), R32 (= R243), G37 (= G246), Q38 (= Q247), T42 (= T251), N47 (= N256), G77 (≠ Q291), V79 (≠ I293), T107 (= T320), G108 (= G321), E155 (≠ A368), V239 (≠ N452), F242 (≠ W455), G272 (= G485), D273 (= D486), Q281 (= Q494), I282 (= I495)
binding nadp nicotinamide-adenine-dinucleotide phosphate: I146 (= I359), G147 (= G360), G148 (= G361), G149 (= G362), D150 (≠ N363), S151 (= S364), E154 (= E367), H170 (≠ E383), R171 (≠ F384), R172 (≠ G385), R176 (= R389), V234 (≠ I447), G235 (= G448), R280 (≠ K493), Q281 (= Q494)

3f8rA Crystal structure of sulfolobus solfataricus thioredoxin reductase b3 in complex with two NADP molecules (see paper)
33% identity, 59% coverage: 206:513/520 of query aligns to 1:305/308 of 3f8rA

query
sites
3f8rA

L

V

K

K

A

P

K

G

D

E

A

K

Y

F

D

D

V

V

L

I

V

I

V
|
V

G
|
G

G
x
L

G
|
G

P
|
P

A
|
A

G

A

A

Y

A

G

A

A

I

L

Y

Y

A

S

A

A

R

R

K

Y

G

M

I

L

R

K

T

T

G

L

V

V

A

I

A

G

E
|
E

R
x
T

F

P

G

G

G
|
G

Q
|
Q

V

L

L

T

D

E

T

A

M

G

A

I

I

V

E

D

N

D

F

Y

I

L

S

G

V

L

K

I

E

E

T

I

E

Q

G

A

P

S

K

D

L

M

A

I

R

K

A

V

L

F

E

N

E

K

H

H

V

I

R

E

E

K

Y

Y

D

E

V

V

D

P

I

V

M

L

N

-

L

-

Q

-

R

-

A

-

S

L

Q

D

L
x
I

I
x
V

P

E

A

K

G

I

D

E

D

N

G

R

L

E

H

F

R

V

V

V

Q

K

F

T

E

K

N

R

G

K

G

G

E

E

L

F

K

K

A

A

K

D

T

S

L

V

I

I

L

L

A
x
G

T
x
I

G
|
G

A

V

R

K

W

R

R
|
R

E

K

M
x
L

N

G

V

V

P

P

G

G

E

E

Q

Q

E

E

Y

F

R

A

G

G

R

R

G

G

V

I

A

S

Y

Y

C
|
C

P

S

H

V

C
|
C

D
|
D

G

A

P

P

L

L

F

F

K

K

G

N

K

R

R

V

V

V

A

A

V

V

I

I

G

G

N
x
D

S
|
S

G

A

V

L

E
|
E

A

G

A

A

I

E

D

I

L

L

A

S

G

S

I

Y

V

S

A

T

H

K

V

V

T

Y

L

L

L

I

E

H

F
x
R

G
x
R

E

D

E

T

L

F

R

K

A

A

D

Q

A

P

V

I

L
x
Y

Q

V

R

E

K

T

L

V

F

K

S

K

L

K

P

P

N

N

V

V

R

E

V

F

L

V

K

L

M

N

A

S

Q

V

T

V

T

K

E

E

V

I

K

K

G

G

D

D

G

-

Q

K

K

V

V

V

T

K

G

Q

L

V

V

V

Y

V

K

E

D

N

R

L

T

K

N

T

D

G

E

E

V

I

H

K

S

E

V

L

E

N

L

V

E

N

G

G

I

V

F

F

V

I

Q

E

I
|
I

G

G

L

F

L

D

P

P

N

P

S

T

E

D

W

F

L

A

K

K

G

S

T

N

-

G

L

I

E

E

L

T

S

D

R

T

F

N

G

G

D

Y

I

I

I

K

V

V

D

D

A

E

K

W

G

M

Q

R

T

T

S

S

L

V

P

P

G

G

V

V

F

F

A

A

G
|
G

D
|
D

V

C

T

T

T

S

-

A

-

W

V
x
L

P
x
G

Y

F

K
x
R

Q

Q

I

V

V

I

I

T

A

A

V

V

G

A

E

Q

G

G

A

A

K

V

A

A

S

A

L

T

S

S

A

A

F

Y

D

R

H

Y

L

V

active site: C133 (= C343), C136 (= C346), D137 (= D347)
binding nadp nicotinamide-adenine-dinucleotide phosphate: V12 (= V217), G13 (= G218), L14 (≠ G219), G15 (= G220), P16 (= P221), A17 (= A222), E37 (= E242), T38 (≠ R243), G41 (= G246), Q42 (= Q247), I82 (≠ L292), V83 (≠ I293), G109 (≠ A319), I110 (≠ T320), G111 (= G321), R115 (= R325), L117 (≠ M327), D153 (≠ N363), S154 (= S364), E157 (= E367), R174 (≠ F384), R175 (≠ G385), Y184 (≠ L394), I236 (= I447), G275 (= G485), D276 (= D486), L282 (≠ V490), G283 (≠ P491), R285 (≠ K493)

5uwyA The crystal structure of thioredoxin reductase from streptococcus pyogenes mgas5005
35% identity, 58% coverage: 212:513/520 of query aligns to 5:303/306 of 5uwyA

query
sites
5uwyA

Y

Y

D

D

V

T

L

L

V

I

G

G

G

S

G
|
G

P
|
P

A
|
A

G

G

A

M

A

T

A

A

I

L

Y

Y

A

A

R

R

K

S

G

N

I

L

R

S

T

V

G

A

V

I

A

I

A
x
E

E
x
Q

R

G

F

A

-

P

G

G

G
|
G

Q
|
Q

V

M

L

N

D

N

T
|
T

M

F

A

D

I

I

E

E

N
|
N

F

Y

I

P

S

G

V

Y

K

D

E

H

T

I

E

S

G

G

P

P

K

E

L

L

A

A

R

M

A

K

L

M

E

Y

E

E

H

P

V

L

R

E

E

K

Y

F

D

N

V

V

D

E

I

-

M

-

N

N

L

I

Q

Y

R

G

A

I

S
x
V

Q

Q

L

K

I

I

P

E

A

-

G

-

D

N

D

F

G

G

L

D

H

Y

R

K

V

C

Q

V

F

L

E

T

N

E

G

D

G

A

E

S

L

Y

K

E

A

A

K

K

T

T

L

V

I

I

L

I

A

A

T
|
T

G
|
G

A

A

R

K

W
x
Y

R

R

E

V

M

L

N

G

V

V

P

P

G

G

E

E

Q

E

E

Y

Y

Y

R

T

G

S

R

R

G

G

V

V

A

S

Y

Y

C

C

P

A

H

V

C
|
C

D

D

G

G

P

A

L

F

F

F

K

R

G

D

K

Q

R

D

V

L

A

L

V

V

I

V

G

G

N

D

S

S

G

A

V

V

E

E

A

E

A

A

I

I

D

Y

L

L

A

T

G

Q

I

F

V

A

A

K

H

K

V

V

T

T

L

V

E

H

F

R

G

R

E

D

E

Q

L

L

R

R

A

A

D

Q

A

K

V

I

L

L

Q

Q

R

D

K

R

L

A

F

F

S

A

L

N

P

D

N

K

V

V

R

D

V

F

L

I

K

W

M

D

A

S

Q

V

T

V

T

K

E

E

V

I

K

Q

G

G

D

N

G

D

Q

I

K

K

V

V

T

S

G

N

L

V

V

L

Y

I

K

E

D

N

R

V

T

K

N

T

D

G

E

Q

V

V

H

T

S

D

V

H

E

A

L

F

E

G

G

G

I

V

F

F

V

I

Q

Y

I

V

G

G

L

M

L

N

P

P

N
x
V

S

T

E

G

W

M

L

V

K

K

G

D

T

L

L

E

E

I

L

T

S

D

R

S

F

E

G

G

D

W

I

I

V

T

D

D

A

D

K

H

G

M

Q

R

T

T

S

S

L

I

P

P

G

G

V

I

F

F

A

A

A

I

G
|
G

D
|
D

V

V

T

R

T

Q

V

K

P

D

Y

L

K

R

Q
|
Q

I
|
I

V

T

I

T

A

A

V

V

G

G

E

D

G

G

A

A

K

I

A

A

S

G

L

Q

S

G

A

V

F

Y

D

H

H

Y

L

L

binding flavin-adenine dinucleotide: G13 (= G220), P14 (= P221), A15 (= A222), E34 (≠ A241), Q35 (≠ E242), G40 (= G246), Q41 (= Q247), T45 (= T251), N50 (= N256), V82 (≠ S290), T110 (= T320), G111 (= G321), Y114 (≠ W324), C136 (= C346), V242 (≠ N452), G275 (= G485), D276 (= D486), Q284 (= Q494), I285 (= I495)

3f8dA Structure of sulfolobus solfataricus thioredoxin reductase mutant c147a (see paper)
33% identity, 59% coverage: 206:513/520 of query aligns to 1:305/308 of 3f8dA

query
sites
3f8dA

L

V

K

K

A

P

K

G

D

E

A

K

Y

F

D

D

V

V

L

I

V

I

V
|
V

G
|
G

G
x
L

G
|
G

P
|
P

A
|
A

G

A

A

Y

A

G

A

A

I

L

Y

Y

A

S

A

A

R

R

K

Y

G

M

I

L

R

K

T

T

G

L

V

V

A

I

A
x
G

E
|
E

R
x
T

F

P

G

G

G
|
G

Q
|
Q

V

L

L

T

D
x
E

T
x
A

M

G

A

I

I
x
V

E

D

N
x
D

F

Y

I

L

S

G

V

L

K

I

E

E

T

I

E

Q

G

A

P

S

K

D

L

M

A

I

R

K

A

V

L

F

E

N

E

K

H

H

V

I

R

E

E

K

Y

Y

D

E

V

V

D

P

I

V

M

L

N

-

L

-

Q

-

R

-

A

-

S

L

Q

D

L
x
I

I
x
V

P

E

A

K

G

I

D

E

D

N

G

R

L

E

H

F

R

V

V

V

Q

K

F

T

E

K

N

R

G

K

G

G

E

E

L

F

K

K

A

A

K

D

T

S

L

V

I

I

L

L

A
x
G

T
x
I

G
|
G

A

V

R

K

W

R

R

R

E

K

M

L

N

G

V

V

P

P

G

G

E

E

Q

Q

E

E

Y

F

R

A

G

G

R

R

G

G

V

I

A

S

Y

Y

C
|
C

P

S

H

V

C
x
A

D
|
D

G

A

P

P

L

L

F

F

K

K

G

N

K

R

R

V

V

V

A

A

V

V

I

I

G

G

N

D

S

S

G

A

V

L

E

E

A

G

A

A

I

E

D

I

L

L

A

S

G

S

I

Y

V

S

A

T

H

K

V

V

T

Y

L

L

L

I

E

H

F

R

G

R

E

D

E

T

L

F

R

K

A

A

D

Q

A

P

V

I

L

Y

Q

V

R

E

K

T

L

V

F

K

S

K

L

K

P

P

N

N

V

V

R

E

V

F

L

V

K

L

M

N

A

S

Q

V

T

V

T

K

E

E

V

I

K

K

G

G

D

D

G

-

Q

K

K

V

V

V

T

K

G

Q

L

V

V

V

Y

V

K

E

D

N

R

L

T

K

N

T

D

G

E

E

V

I

H

K

S

E

V

L

E

N

L

V

E

N

G

G

I

V

F

F

V

I

Q

E

I

I

G

G

L

F

L

D

P

P

N

P

S

T

E

D

W

F

L

A

K

K

G

S

T

N

-

G

L

I

E

E

L

T

S

D

R

T

F

N

G

G

D

Y

I

I

I

K

V

V

D

D

A

E

K

W

G

M

Q

R

T

T

S

S

L

V

P

P

G

G

V

V

F

F

A

A

G
|
G

D
|
D

V

C

T

T

T

S

-

A

-

W

V

L

P

G

Y

F

K
x
R

Q
|
Q

I
x
V

V

I

I

T

A

A

V

V

G

A

E

Q

G

G

A

A

K

V

A

A

S

A

L

T

S

S

A

A

F

Y

D

R

H

Y

L

V

active site: C133 (= C343), A136 (≠ C346), D137 (= D347)
binding flavin-adenine dinucleotide: V12 (= V217), G13 (= G218), L14 (≠ G219), G15 (= G220), P16 (= P221),