Comparing GFF63 FitnessBrowser__Marino:GFF63 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 19 hits to proteins with known functional sites (download)
1qs0B Crystal structure of pseudomonas putida 2-oxoisovalerate dehydrogenase (branched-chain alpha-keto acid dehydrogenase, e1b) (see paper)
70% identity, 100% coverage: 1:267/268 of query aligns to 71:337/338 of 1qs0B
Sites not aligning to the query:
2j9fD Human branched-chain alpha-ketoacid dehydrogenase-decarboxylase e1b (see paper)
47% identity, 100% coverage: 1:268/268 of query aligns to 73:329/329 of 2j9fD
Sites not aligning to the query:
1dtwB Human branched-chain alpha-keto acid dehydrogenase (see paper)
47% identity, 100% coverage: 1:268/268 of query aligns to 70:326/326 of 1dtwB
Sites not aligning to the query:
P21953 2-oxoisovalerate dehydrogenase subunit beta, mitochondrial; Branched-chain alpha-keto acid dehydrogenase E1 component beta chain; BCKDE1B; BCKDH E1-beta; EC 1.2.4.4 from Homo sapiens (Human) (see 2 papers)
47% identity, 100% coverage: 1:268/268 of query aligns to 136:392/392 of P21953
1umdD Branched-chain 2-oxo acid dehydrogenase (e1) from thermus thermophilus hb8 with 4-methyl-2-oxopentanoate as an intermediate (see paper)
49% identity, 100% coverage: 1:268/268 of query aligns to 69:323/323 of 1umdD
Sites not aligning to the query:
1umcD Branched-chain 2-oxo acid dehydrogenase (e1) from thermus thermophilus hb8 with 4-methylpentanoate (see paper)
49% identity, 100% coverage: 1:268/268 of query aligns to 69:323/323 of 1umcD
Sites not aligning to the query:
1umbD Branched-chain 2-oxo acid dehydrogenase (e1) from thermus thermophilus hb8 in holo-form (see paper)
49% identity, 100% coverage: 1:268/268 of query aligns to 69:323/323 of 1umbD
Sites not aligning to the query:
Q5SLR3 2-oxoisovalerate dehydrogenase subunit beta; Branched-chain alpha-keto acid dehydrogenase E1 component beta chain; BCKDH E1-beta; EC 1.2.4.4 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see paper)
49% identity, 100% coverage: 1:268/268 of query aligns to 70:324/324 of Q5SLR3
Sites not aligning to the query:
3dv0D Snapshots of catalysis in the e1 subunit of the pyruvate dehydrogenase multi-enzyme complex (see paper)
44% identity, 100% coverage: 1:267/268 of query aligns to 69:323/324 of 3dv0D
Sites not aligning to the query:
3dv0B Snapshots of catalysis in the e1 subunit of the pyruvate dehydrogenase multi-enzyme complex (see paper)
44% identity, 100% coverage: 1:267/268 of query aligns to 69:323/324 of 3dv0B
Sites not aligning to the query:
3dufD Snapshots of catalysis in the e1 subunit of the pyruvate dehydrogenase multi-enzyme complex (see paper)
44% identity, 100% coverage: 1:267/268 of query aligns to 69:323/324 of 3dufD
Sites not aligning to the query:
1w85B The crystal structure of pyruvate dehydrogenase e1 bound to the peripheral subunit binding domain of e2 (see paper)
44% identity, 100% coverage: 1:267/268 of query aligns to 69:323/324 of 1w85B
Sites not aligning to the query:
P11177 Pyruvate dehydrogenase E1 component subunit beta, mitochondrial; PDHE1-B; EC 1.2.4.1 from Homo sapiens (Human) (see 6 papers)
34% identity, 98% coverage: 5:267/268 of query aligns to 103:358/359 of P11177
Sites not aligning to the query:
6cfoB Human pyruvate dehydrogenase e1 component complex with covalent tdp adduct acetyl phosphinate (see paper)
34% identity, 98% coverage: 5:267/268 of query aligns to 74:329/330 of 6cfoB
Sites not aligning to the query:
6cerD Human pyruvate dehydrogenase complex e1 component v138m mutation (see paper)
34% identity, 98% coverage: 5:267/268 of query aligns to 75:330/331 of 6cerD
Sites not aligning to the query:
6yakDDD C-terminal component of the split chain transketolase (see paper)
28% identity, 47% coverage: 141:267/268 of query aligns to 179:308/311 of 6yakDDD
Sites not aligning to the query:
8bzxB 1-deoxy-d-xylulose 5-phosphate synthase from klebsiella pneumoniae (kpdxps),co-crystal with thiamine monophosphate analog (see paper)
23% identity, 80% coverage: 7:221/268 of query aligns to 334:521/568 of 8bzxB
Sites not aligning to the query:
8bzxA 1-deoxy-d-xylulose 5-phosphate synthase from klebsiella pneumoniae (kpdxps),co-crystal with thiamine monophosphate analog (see paper)
23% identity, 80% coverage: 7:221/268 of query aligns to 312:499/546 of 8bzxA
Sites not aligning to the query:
8a9cB Structure of truncated 1-deoxy-d-xylulose 5-phosphate synthase (dxs) from klebsiella pneumoniae in complex with cofactor tpp
23% identity, 81% coverage: 7:222/268 of query aligns to 287:475/520 of 8a9cB
Sites not aligning to the query:
>GFF63 FitnessBrowser__Marino:GFF63
MGAYGLRPVAEIQFADYILPAYDQLVSEAARLRYRSGGEFWAPITVRSPYGGGIFGGQTH
SQSPEAIFAHITGLKTVIPSNPYDAKGLLISSIESDDPIIFLEPKRIYNGPFDGHHERQI
KSWADHPDASVPEGHYTVPLGKAATVRHGSDVTVLAYGAMVHVAKAGIEESGVDAELLDL
RSIVPLDIDAIVQSVKKTGRCVILHEASRYGGFGGELSALVQERCFYHLKSPIERVAGWD
TPYPHAFEWDYFPGPMRLAKALQKSMNH
Or try a new SitesBLAST search
SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.
Lawrence Berkeley National Laboratory