SitesBLAST
Comparing GFF641 PGA1_c06550 phosphoglucosamine mutase GlmM to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
P31120 Phosphoglucosamine mutase; EC 5.4.2.10 from Escherichia coli (strain K12) (see 3 papers)
48% identity, 99% coverage: 2:444/448 of query aligns to 4:444/445 of P31120
- S100 (= S100) mutation to A: 2% of wild-type activity.; mutation to T: 20-fold increase in the non-specific phosphoglucomutase activity towards glucose-phosphate substrates (non aminated).
- S102 (= S102) active site, Phosphoserine intermediate; modified: Phosphoserine; by autocatalysis; mutation to A: Loss of activity in the absence or presence of glucosamine-1,6-diP.
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
7omlA Bacillus subtilis phosphoglucomutase glmm (metal bound) (see paper)
44% identity, 99% coverage: 3:444/448 of query aligns to 2:442/445 of 7omlA
7ojrA Bacillus subtilis phosphoglucomutase glmm (phosphate bound) (see paper)
44% identity, 99% coverage: 3:444/448 of query aligns to 2:442/445 of 7ojrA
3i3wA Structure of a phosphoglucosamine mutase from francisella tularensis
38% identity, 98% coverage: 3:443/448 of query aligns to 1:437/441 of 3i3wA
- active site: R9 (= R11), S99 (= S102), H100 (= H103), K109 (= K112), D237 (= D241), D239 (= D243), D241 (= D245), R242 (= R246), H324 (= H330)
- binding zinc ion: S99 (= S102), D237 (= D241), D239 (= D243), D241 (= D245)
1wqaA Crystal structure of pyrococcus horikoshii phosphomannomutase/phosphoglucomutase complexed with mg2+
33% identity, 100% coverage: 1:447/448 of query aligns to 1:455/455 of 1wqaA
- active site: R11 (= R11), S101 (= S102), H102 (= H103), K111 (= K112), D243 (= D241), D245 (= D243), D247 (= D245), R248 (= R246), G330 (≠ H330), R340 (≠ G340)
- binding magnesium ion: S101 (= S102), D243 (= D241), D245 (= D243), D247 (= D245)
2h5aX Complex of the enzyme pmm/pgm with xylose 1-phosphate (see paper)
30% identity, 77% coverage: 19:362/448 of query aligns to 19:353/455 of 2h5aX
- active site: H101 (= H103), D234 (= D241), D236 (= D243), D238 (= D245), R239 (= R246), D332 (≠ G340)
- binding 1-O-phosphono-alpha-D-xylopyranose: T298 (≠ V307), G299 (= G308), H300 (≠ D309), E317 (= E326), S319 (= S328), H321 (= H330)
- binding zinc ion: S100 (= S102), D234 (= D241), D236 (= D243), D238 (= D245)
Sites not aligning to the query:
2h4lX Complex of pmm/pgm with ribose 1-phosphate (see paper)
30% identity, 77% coverage: 19:362/448 of query aligns to 19:353/455 of 2h4lX
- active site: H101 (= H103), D234 (= D241), D236 (= D243), D238 (= D245), R239 (= R246), D332 (≠ G340)
- binding 1-O-phosphono-alpha-D-ribofuranose: S100 (= S102), K277 (≠ M286), T298 (≠ V307), G299 (= G308), E317 (= E326), S319 (= S328)
- binding zinc ion: S100 (= S102), D234 (= D241), D236 (= D243), D238 (= D245)
Sites not aligning to the query:
2fkfA Phosphomannomutase/phosphoglucomutase from pseudomonas aeruginosa with alpha-d-glucose 1,6-bisphosphate bound (see paper)
30% identity, 77% coverage: 19:362/448 of query aligns to 19:353/455 of 2fkfA
- active site: S100 (= S102), H101 (= H103), K110 (= K112), D234 (= D241), D236 (= D243), D238 (= D245), R239 (= R246), H321 (= H330), D332 (≠ G340)
- binding 1,6-di-O-phosphono-alpha-D-glucopyranose: H101 (= H103), N102 (= N104), S319 (= S328)
- binding zinc ion: S100 (= S102), D234 (= D241), D236 (= D243), D238 (= D245)
Sites not aligning to the query:
1pcmX Enzyme-ligand complex of p. Aeruginosa pmm/pgm (see paper)
30% identity, 77% coverage: 19:362/448 of query aligns to 19:353/455 of 1pcmX
- active site: S100 (= S102), H101 (= H103), K110 (= K112), D234 (= D241), D236 (= D243), D238 (= D245), R239 (= R246), H321 (= H330), D332 (≠ G340)
- binding 6-O-phosphono-alpha-D-mannopyranose: S100 (= S102), K277 (≠ M286), T298 (≠ V307), G299 (= G308), H300 (≠ D309), E317 (= E326), S319 (= S328), H321 (= H330)
- binding zinc ion: S100 (= S102), D234 (= D241), D236 (= D243), D238 (= D245)
Sites not aligning to the query:
1p5gX Enzyme-ligand complex of p. Aeruginosa pmm/pgm (see paper)
30% identity, 77% coverage: 19:362/448 of query aligns to 19:353/455 of 1p5gX
- active site: S100 (= S102), H101 (= H103), K110 (= K112), D234 (= D241), D236 (= D243), D238 (= D245), R239 (= R246), H321 (= H330), D332 (≠ G340)
- binding 6-O-phosphono-alpha-D-glucopyranose: S100 (= S102), K277 (≠ M286), T298 (≠ V307), G299 (= G308), H300 (≠ D309), E317 (= E326), S319 (= S328), H321 (= H330)
- binding zinc ion: S100 (= S102), D234 (= D241), D236 (= D243), D238 (= D245)
Sites not aligning to the query:
1p5dX Enzyme-ligand complex of p. Aeruginosa pmm/pgm (see paper)
30% identity, 77% coverage: 19:362/448 of query aligns to 19:353/455 of 1p5dX
- active site: S100 (= S102), H101 (= H103), K110 (= K112), D234 (= D241), D236 (= D243), D238 (= D245), R239 (= R246), H321 (= H330), D332 (≠ G340)
- binding 1-O-phosphono-alpha-D-glucopyranose: S100 (= S102), R239 (= R246), K277 (≠ M286), T298 (≠ V307), G299 (= G308), H300 (≠ D309), E317 (= E326), S319 (= S328), H321 (= H330)
- binding zinc ion: S100 (= S102), D234 (= D241), D236 (= D243), D238 (= D245)
Sites not aligning to the query:
1pcjX Enzyme-ligand complex of p. Aeruginosa pmm/pgm (see paper)
30% identity, 77% coverage: 19:362/448 of query aligns to 22:356/458 of 1pcjX