SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF707 PGA1_c07220 putative 6-phospho-5-dehydro-2-deoxy-D-gluconate aldolase to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

8q59A Crystal structure of metal-dependent class ii sulfofructose phosphate aldolase from yersinia aldovae in complex with sulfofructose phosphate (yasqia-zn-sfp) (see paper)
37% identity, 86% coverage: 9:249/279 of query aligns to 9:246/280 of 8q59A

query
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8q59A

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binding (3~{S},4~{S})-2,3,4-tris(oxidanyl)-5-oxidanylidene-6-phosphonooxy-hexane-1-sulfonic acid: G50 (= G50), Q51 (≠ P51), K52 (≠ S52), D82 (= D82), H83 (= H83), H172 (= H174), G173 (≠ L175), H200 (= H202), G201 (= G203), S203 (= S205), N222 (= N225), D224 (≠ G227), T225 (= T228)
binding zinc ion: H83 (= H83), H172 (= H174), H200 (= H202)

3q94A The crystal structure of fructose 1,6-bisphosphate aldolase from bacillus anthracis str. 'Ames ancestor'
35% identity, 98% coverage: 1:273/279 of query aligns to 1:279/285 of 3q94A

query
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3q94A

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active site: D85 (= D82), H86 (= H83), E145 (≠ G142), H181 (= H174), H209 (= H202), N231 (= N225)
binding zinc ion: H86 (= H83), E114 (≠ D111), H163 (≠ R156), H181 (= H174), H209 (= H202), E235 (= E229), E239 (≠ V233)

5ud0A Class ii fructose-1,6-bisphosphate aldolase e149a variant of helicobacter pylori with cleavage products (see paper)
32% identity, 95% coverage: 8:271/279 of query aligns to 7:270/282 of 5ud0A

query
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5ud0A

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binding zinc ion: H83 (= H83), E134 (= E134), H163 (= H174), H185 (= H202)

8q5aA Crystal structure of metal-dependent class ii sulfofructosephosphate aldolase from hafnia paralvei hpsqia-zn in complex with dihydroxyacetone phosphate (dhap) (see paper)
36% identity, 86% coverage: 9:249/279 of query aligns to 9:246/276 of 8q5aA

query
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8q5aA

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binding zinc ion: H83 (= H83), H83 (= H83), H172 (= H174), H200 (= H202), H200 (= H202)

3gayA Structure of giardia fructose-1,6-biphosphate aldolase in complex with tagatose-1,6-biphosphate (see paper)
30% identity, 97% coverage: 3:273/279 of query aligns to 2:297/319 of 3gayA

query
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3gayA

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S

H
|
H

-
x
G

-

A

-

Y

-
x
K

L

F

Q

K

Q

S

D

E

K

S

E

D

G

I

G

R

L

L

D

A

I

I

D

D

R

R

I

V

R

K

A

T

I

I

E

S

A

D

I

L

T

T

E

G

V

I

P

P

L

L

V

V

I

M

H
|
H

G
|
G

G

S

S
|
S

G

S

V

V

P

P

V

K

A

D

Q

V

R

K

R

D

M

M

L

I

A

N

R

K

-

Y

-

G

-

K

-

M

-

P

-

D

-

A

-

V

-

G

-

V

-

P

-

I

E

E

S

S

R

I

-

V

-

H

-

A

-

I

-

G

-

E

-

G

I

V

C

C

K

K

F

I

N
|
N

I

V

G
x
D

T
x
S

E

D

L

S

R
|
R

M

M

V

A

F

M

G

T

A

G

A

A

M

I

R

R

D

K

A

V

V

F

N

V

S

E

D

H

P

P

D

E

R

K

F

F

D

D

R

P

V

R

S

D

I

Y

L

L

S

G

E

P

T

G

H

R

D

D

P

A

V

I

V

T

A

E

A

M

A

L

R

I

S

P

V

K

L

I

R

K

A

A

F

F

binding 1,6-di-O-phosphono-D-tagatose: N23 (≠ V24), S49 (≠ G50), D82 (= D82), H174 (= H174), G175 (vs. gap), K178 (vs. gap), H206 (= H202), G207 (= G203), S209 (= S205), N249 (= N225), D251 (≠ G227), S252 (≠ T228), R255 (= R231)
binding zinc ion: H83 (= H83), H174 (= H174), H206 (= H202)

P13243 Probable fructose-bisphosphate aldolase; FBP aldolase; FBPA; Fructose-1,6-bisphosphate aldolase; EC 4.1.2.13 from Bacillus subtilis (strain 168) (see paper)
33% identity, 100% coverage: 1:278/279 of query aligns to 1:284/285 of P13243

query
sites
P13243

M

M

T

P

I

L

A

V

T

S

L

M

A

T

E

E

V

M

L

L

Q

N

P

T

A

A

L

K

R

E

D

K

G

G

Y

Y

A

A

V

V

A

G

G

Q

L

F

V

N

T

L

L

N

G

N

W

L

E

E

D

F

M

T

R

Q

A

A

Y

I

V

L

A

Q

A

A

E

E

A

E

E

E

G

K

V

S

P

P

V

V

I

I

L

L

Q

G

A

V

G

S

P

E

S

G

C

A

-

G

R

R

V

Y

H

M

T

G

P

G

L

F

P

K

I

T

L

V

G

V

K

A

M

M

F

V

R

K

H

A

L

L

A

M

E

E

-

E

-

Y

G

K

A

V

S

T

V

V

P

P

V

V

V

A

A

I

H

H

L

L

D

D

H

H

G

G

Y

S

T

S

A

F

E

E

D

S

C

C

R

A

I

K

A

A

I

I

D

H

S

A

G

G

F

F

T

T

S

S

V

V

M

M

F

I

D

D

G

A

S

S

R

H

N

H

A

P

L

F

D

E

N

N

I

V

A

A

E

T

T

T

A

A

A

K

I

V

A

V

G

E

M

L

A

A

H

H

A

F

A

H

G

G

V

V

S

S

C

V

E

E

G

A

E

E

I

L

G

G

F

T

V

V

G

G

Y

G

S

Q

G

E

-

D

-

V

-

I

G

A

E

E

G

G

S

V

A

I

G

Y

T

A

D

D

P

P

E

K

E

E

A

C

R

Q

R

E

F

L

A

V

Q

E

E

R

T

T

G

G

V

I

D

D

A

C

M

L

A

A

I

P

S

A

V

L

G

G

N

S

V

V

H

H

L

G

Q

P

Q

Y

D

K

K

G

E

E

G

P

G

N

L

L

D

G

I

F

D

K

R

E

I

M

R

E

A

E

I

I

E

G

A

K

I

S

T

T

E

G

V

L

P

P

L

L

V

V

I

L

H

H

G

G

S
x
T

G

G

V

I

P

P

V

T

A

A

Q

D

-

I

R

K

M

S

L

I

A

S

R

L

E

G

S

T

R

-

I

-

C

A

K

K

F

I

N

N

I

V

G

N

T
|
T

E

E

L

N

R

Q

M

I

V

S

F

S

G

A

A

K

A

A

M

V

R

R

D

E

A

T

V

L

N

A

S

A

D

K

P

P

D

D

R

E

F

Y

D

D

R

P

V

R

S

K

I

Y

L

L

S

G

E

P

T

A

H

R

D

E

P

A

V

I

V

K

A

E

A

T

A

V

R

I

S

G

V

K

L

M

R

R

A

E

F

F

K

G

G

S

E

S

T

N

Q

Q

T212 (≠ S205) modified: Phosphothreonine
T234 (= T228) modified: Phosphothreonine

A8B2U2 Fructose-bisphosphate aldolase; Glfba; glFBPA; Fructose-1,6-bisphosphate aldolase; EC 4.1.2.13 from Giardia intestinalis (strain ATCC 50803 / WB clone C6) (Giardia lamblia) (see 4 papers)
31% identity, 98% coverage: 1:273/279 of query aligns to 1:301/323 of A8B2U2

query
sites
A8B2U2

M

M

T

P

I

L

A

C

T

T

L

L

A

R

E

Q

V

M

L

L

Q

G

P

E

A

A

L

R

R

K

D

H

G

K

Y

Y

A

G

V

V

A

G

G

A

L

F

V

N

T

V

L

N

G

N

W

M

E

E

D

Q

M

I

R

Q

A

G

Y

I

V

M

A

K

A

A

A

V

E

V

A

Q

E

L

G

K

V

S

P

P

V

V

I

I

L

L

Q

Q

A

C

G
x
S

P

R

S

G

C

A

R

L

V

K

H

Y

T

S

P

D

L

M

P

I

I

Y

L

L

G

K

K

K

M

L

F

C

R

E

H

A

-

A

L

L

A

E

E

K

G

H

A

P

S

D

V

I

P

P

V

I

V

C

A

I

H

H

L

L

D
|
D

H
|
H

G

G

Y

D

T

T

A

L

E

E

D

S

C

V

R

K

I

M

A

A

I

I

D

D

S

L

G

G

F

F

T

S

S

S

V

V

M

M

F

I

D

D

G

A

S

S

R

H

N

H

A

P

L

F

D

D

D

E

N

N

I

V

A

R

E

I

T

T

A

K

A

E

I

V

A

V

G

A

M

Y

A

A

H

H

A

A

A

R

G

G

V

V

S

S

C

V

E

E

G

A

E

E

I

L

G

G

F

T

V

L

G

G

Y

-

S

-

G

G

G

I

E

E

G

E

S

D

A

V

G

Q

-

N

-

T

-

V

-

Q

-

L

T

T

D

E

P

P

E

Q

E

D

A

A

R

K

F

F

A

V

Q

E

E

L

T

T

G

G

V

V

D

D

A

A

M

L

A

A

I

V

S

A

V

I

G

G

N

T

V

S

H
|
H

-
x
G

-

A

-

Y

-
x
K

L

F

Q

K

Q

S

D

E

K

S

E

D

G

I

G

R

L

L

D

A

I

I

D

D

R

R

I

V

R

K

A

T

I

I

E

S

A

D

I

L

T

T

E

G

V

I

P

P

L

L

V

V

I

M

H
|
H

G
|
G

G

S

S
|
S

G

S

V

V

P

P

V

K

A

D

Q

V

R

K

R

D

M

M

L

I

A

N

R

K

-

Y

-

G

-

K

-

M

-

P

-

D

-

A

-

V

-

G

-

V

-

P

-

I

E

E

S

S

R

I

-

V

-

H

-

A

-

I

-

G

-

E

-

G

I

V

C

C

K

K

F

I

N
|
N

I

V

G
x
D

T
x
S

E

D

L

S

R
|
R

M

M

V

A

F

M

G

T

A

G

A

A

M

I

R

R

D

K

A

V

V

F

N

V

S

E

D

H

P

P

D

E

R

K

F

F

D
|
D

R

P

V
x
R

S

D

I

Y

L

L

S

G

E

P

T

G

H

R

D

D

P

A

V

I

V

T

A

E

A

M

A

L

R

I

S

P

V

K

L

I

R

K

A

A

F

F

S50 (≠ G50) binding
D83 (= D82) active site, Proton donor; mutation to A: Severe loss of catalytic activity.
H84 (= H83) binding
H178 (= H174) binding ; binding
G179 (vs. gap) binding
K182 (vs. gap) binding
H210 (= H202) binding
G211 (= G203) binding
S213 (= S205) binding
N253 (= N225) binding
D255 (≠ G227) binding ; mutation to A: 9.4-fold reduction in substrate affinity and 50-fold reduction in catalytic affinity. Has some activity towards tagatose-1,6-bisphosphate.
S256 (≠ T228) binding
R259 (= R231) binding ; mutation to A: 1.8-fold reduction in substrate affinity and 2.8-fold reduction in catalytic efficiency. 6-fold reduction in substrate affinity and 24-fold reduction in catalytic efficiency; when associated with A-278.
D278 (= D250) mutation to A: 159-fold reduction in substrate affinity and 2770-fold reduction in catalytic efficiency. 6-fold reduction in substrate affinity and 24-fold reduction in catalytic efficiency; when associated with A-259.
R280 (≠ V252) binding

3ohiA Structure of giardia fructose-1,6-biphosphate aldolase in complex with 3-hydroxy-2-pyridone (see paper)
30% identity, 97% coverage: 3:273/279 of query aligns to 2:297/319 of 3ohiA

query
sites
3ohiA

I

L

A

C

T

T

L

L

A

R

E

Q

V

M

L

L

Q

G

P

E

A

A

L

R

R

K

D

H

G

K

Y

Y

A

G

V

V

A

G

G

A

L

F

V

N

T

V

L

N

G

N

W

M

E

E

D

Q

M

I

R

Q

A

G

Y

I

V

M

A

K

A

A

A

V

E

V

A

Q

E

L

G

K

V

S

P

P

V

V

I

I

L

L

Q

Q

A

C

G
x
S

P

R

S

G

C

A

R

L

V

K

H

Y

T

S

P

D

L

M

P

I

I

Y

L

L

G

K

K

K

M

L

F

C

R

E

H

A

-

A

L

L

A

E

E

K

G

H

A

P

S

D

V

I

P

P

V

I

V

C

A

I

H

H

L

L

D
|
D

H
|
H

G

G

Y

D

T

T

A

L

E

E

D

S

C

V

R

K

I

M

A

A

I

I

D

D

S

L

G

G

F

F

T

S

S

S

V

V

M

M

F

I

D

D

G

A

S

S

R

H

N

H

A

P

L

F

D

D

D

E

N

N

I

V

A

R

E

I

T

T

A

K

A

E

I

V

A

V

G

A

M

Y

A

A

H

H

A

A

A

R

G

S

V

V

S

S

C

V

E

E

G

A

E

E

I

L

G

G

F

T

V

L

G

G

Y

G

S

I

G

E

G

Q

E

N

G

T

S

V

A

Q

G

L

T

T

D

E

P

P

E

Q

E

D

A

A

R

K

F

F

A

V

Q

E

E

L

T

T

G

G

V

V

D

D

A

A

M

L

A

A

I

V

S

A

V

I

G

G

N

T

V

S

H
|
H

-
x
G

-

A

-

Y

-
x
K

L

F

Q

K

Q

S

D

E

K

S

E

D

G

I

G

R

L

L

D

A

I

I

D

D

R

R

I

V

R

K

A

T

I

I

E

S

A

D

I

L

T

T

E

G

V

I

P

P

L

L

V

V

I

M

H
|
H

G
|
G

G

S

S
|
S

G

S

V

V

P

P

V

K

A

D

Q

V

R

K

R

D

M

M

L

I

A

N

R

K

-

Y

-

G

-

K

-

M

-

P

-

D

-

A

-

V

-

G

-

V

-

P

-

I

E

E

S

S

R

I

-

V

-

H

-

A

-

I

-

G

-

E

-

G

I

V

C

C

K

K

F

I

N
|
N

I

V

G
x
D

T
x
S

E

D

L

S

R
|
R

M

M

V

A

F

M

G

T

A

G

A

A

M

I

R

R

D

K

A

V

V

F

N

V

S

E

D

H

P

P

D

E

R

K

F

F

D

D

R

P

V

R

S

D

I

Y

L

L

S

G

E

P

T

G

H

R

D

D

P

A

V

I

V

T

A

E

A

M

A

L

R

I

S

P

V

K

L

I

R

K

A

A

F

F

binding ({3-hydroxy-2-oxo-4-[2-(phosphonooxy)ethyl]pyridin-1(2H)-yl}methyl)phosphonic acid: S49 (≠ G50), D82 (= D82), H83 (= H83), H174 (= H174), G175 (vs. gap), K178 (vs. gap), G207 (= G203), S209 (= S205), N249 (= N225), D251 (≠ G227), S252 (≠ T228), R255 (= R231)
binding zinc ion: H83 (= H83), H174 (= H174), H206 (= H202)

3c52A Class ii fructose-1,6-bisphosphate aldolase from helicobacter pylori in complex with phosphoglycolohydroxamic acid, a competitive inhibitor (see paper)
31% identity, 95% coverage: 8:271/279 of query aligns to 7:284/296 of 3c52A

query
sites
3c52A

E

E

V

I

L

L

Q

L

P

K

A

A

L

H

R

K

D

E

G

G

Y

Y

A

G

V

V

A

G

G

A

L

F

V

N

T

F

L

V

G

N

W

F

E

E

D

M

M

L

R

N

A

A

Y

I

V

F

A

E

A

A

A

G

E

N

A

E

E

E

G

N

V

S

P

P

V

L

I

F

L

I

Q

Q

A

A

G

S

P

E

S

G

C

A

R

I

V

K

H

Y

T

M

P

G

L

I

P

D

I

M

L

A

G

V

K

G

M

M

F

V

R

K

H

I

L

M

A
x
C

E
|
E

-

R

G

Y

A

P

S

H

V

I

P

P

V

V

V

A

A

L

H

H

L

L

D
|
D

H
|
H

G

G

Y

T

T

T

A

F

E

E

D

S

C

C

R

E

I

K

A

A

I

V

D

K

S

A

G

G

F

F

T

T

S

S

V

V

M

M

F

I

D
|
D

G

A

S
|
S

R

H

N

H

A

A

L

F

D

E

N

N

I

L

A

E

E

L

T

T

A

S

A

K

I

V

A

V

G

K

M

M

A

A

H

H

A

N

A

A

G

G

V

V

S

S

C

V

E

E

G

A

E
|
E

I

L

G
|
G

F

R

V

L

G

-

Y

-

S

-

G

-

E

M

G

G

S

I

A

V

G

L

T

V

D

N

P

P

E

K

E

E

A

A

R

E

R

Q

F

F

A

V

Q

K

E

E

T

S

G

Q

V

V

D

D

A

Y

M

L

A

A

I

P

S

A

V

I

G

G

N

T

V

S

H
|
H

-

G

-

A

L

F

Q
x
K

Q

F

D

K

K

G

E

E

G

P

G

K

L

L

D

D

I

F

D

E

R

R

I

L

R

Q

A

E

I

V

E

K

A

R

I

L

T

T

E

N

V

I

P

P

L

L

V

V

I

L

H
|
H

G
|
G

G

A

S
|
S

G

A

V

I

P

P

V

D

A

N

Q

V

R

R

R

K

M

S

-

Y

-

L

-

D

-
x
A

-

G

-

D

-

L

-

K

-

G

-

S

-

K

-

G

-

V

-

P

-

F

-

E

L

F

A

L

R

Q

E

E

S

S

R

V

-

K

-

G

I

I

C

N

K

K

F

V

N
|
N

I

T

G
x
D

T
|
T

E

D

L

L

R

R

M

I

V

A

F

F

G

I

A

A

A

E

M

V

R

R

D

K

A

V

V

A

N

N

S

E

D

D

P

K

D

S

R

Q

F

F

D

D

R

-

V

-

S

-

I

-

L

L

S

R

E

K

T

F

H

F

D

S

P

P

V

A

V

Q

A

L

A

A

A

L

R

K

S

N

V

V

L

V

R

K

active site: C69 (≠ A70), E70 (= E71), G136 (= G136), H169 (= H174), A215 (vs. gap), N242 (= N225)
binding calcium ion: D104 (= D104), S106 (= S106), E134 (= E134)
binding phosphoglycolohydroxamic acid: D82 (= D82), H83 (= H83), H169 (= H174), K173 (≠ Q176), H199 (= H202), G200 (= G203), S202 (= S205), N242 (= N225), D244 (≠ G227), T245 (= T228)
binding zinc ion: H83 (= H83), H169 (= H174), H199 (= H202)

5uckA Class ii fructose-1,6-bisphosphate aldolase of helicobacter pylori with cleavage products (see paper)
31% identity, 95% coverage: 8:271/279 of query aligns to 7:279/291 of 5uckA

query
sites
5uckA

E

E

V

I

L

L

Q

L

P

K

A

A

L

H

R

K

D

E

G

G

Y

Y

A

G

V

V

A

G

G

A

L

F

V

N

T

F

L

V

G

N

W

F

E

E

D

M

M

L

R

N

A

A

Y

I

V

F

A

E

A

A

A

G

E

N

A

E

E

E

G

N

V

S

P

P

V

L

I

F

L

I

Q

Q

A

A

G
x
S

P

E

S

G

C

A

R

I

V

K

H

Y

T

M

P

G

L

I

P

D

I

M

L

A

G

V

K

G

M

M

F

V

R

K

H

I

L

M

A

C

E

E

-

R

G

Y

A

P

S

H

V

I

P

P

V

V

V

A

A

L

H

H

L

L

D
|
D

H
|
H

G

G

Y

T

T

T

A

F

E

E

D

S

C

C

R

E

I

K

A

A

I

V

D

K

S

A

G

G

F

F

T

T

S

S

V

V

M

M

F

I

D

D

G

A

S

S

R

H

N

H

A

A

L

F

D

E

N

N

I

L

A

E

E

L

T

T

A

S

A

K

I

V

A

V

G

K

M

M

A

A

H

H

A

N

A

A

G

G

V

V

S

S

C

V

E

E

G

A

E
|
E

I

L

G

G

F

R

V

L

G

-

Y

-

S

-

G

-

E

-

G

-

S

-

A

-

G

-

T

V

D

N

P

P

E

K

E

E

A

A

R

E

R

Q

F

F

A

V

Q

K

E

E

T

S

G

Q

V

V

D

D

A

Y

M

L

A

A

I

P

S

A

V

I

G

G

N

T

V

S

H
|
H

-

G

-

A

L

F

Q

K

Q

F

D

K

K

G

E

E

G

P

G

K

L

L

D

D

I

F

D

E

R

R

I

L

R

Q

A

E

I

V

E

K

A

R

I

L

T

T

E

N

V

I

P

P

L

L

V

V

I

L

H
|
H

G

G

G

A

S

S

G

A

V

I

P

P

V

D

A

N

Q

V

R

R

R

K

M

S

-

Y

-

L

-

D

-

A

-

G

-

D

-

L

-

K

-

G

-

S

-

K

-

G

-

V

-

P

-

F

-

E

L

F

A

L

R

Q

E

E

S

S

R

V

-

K

-

G

I

I

C

N

K

K

F

V

N

N

I

T

G
x
D

T

T

E

D

L

L

R
|
R

M

I

V

A

F

F

G

I

A

A

A

E

M

V

R

R

D

K

A

V

V

A

N

N

S

E

D

D

P

K

D

S

R

Q

F

F

D

D

R

-

V

-

S

-

I

-

L

L

S

R

E

K

T

F

H

F

D

S

P

P

V

A

V

Q

A

L

A

A

A

L

R

K

S

N

V

V

L

V

R

K

binding glyceraldehyde-3-phosphate: S49 (≠ G50), D82 (= D82), H83 (= H83), H164 (= H174), D239 (≠ G227), R243 (= R231)
binding zinc ion: H83 (= H83), H83 (= H83), E134 (= E134), H164 (= H174), H194 (= H202), H194 (= H202)

5ucpA Class ii fructose-1,6-bisphosphate aldolase e142a variant of helicobacter pylori with fbp and cleavage products (see paper)
31% identity, 95% coverage: 8:271/279 of query aligns to 7:280/292 of 5ucpA

query
sites
5ucpA

E

E

V

I

L

L

Q

L

P

K

A

A

L

H

R

K

D

E

G

G

Y

Y

A

G

V

V

A

G

G

A

L

F

V

N

T

F

L

V

G

N

W

F

E

E

D

M

M

L

R

N

A

A

Y

I

V

F

A

E

A

A

A

G

E

N

A

E

E

E

G

N

V

S

P

P

V

L

I

F

L

I

Q

Q

A

A

G
x
S

P

E

S

G

C

A

R

I

V

K

H

Y

T

M

P

G

L

I

P

D

I

M

L

A

G

V

K

G

M

M

F

V

R

K

H

I

L

M

A

C

E

E

-

R

G

Y

A

P

S

H

V

I

P

P

V

V

V

A

A

L

H

H

L

L

D
|
D

H
|
H

G

G

Y

T

T

T

A

F

E

E

D

S

C

C

R

E

I

K

A

A

I

V

D

K

S

A

G

G

F

F

T

T

S

S

V

V

M

M

F

I

D

D

G

A

S

S

R

H

N

H

A

A

L

F

D

E

N

N

I

L

A

E

E

L

T

T

A

S

A

K

I

V

A

V

G

K

M

M

A

A

H

H

A

N

A

A

G

G

V

V

S

S

C

V

E

E

G

A

E
|
E

I

L

G

G

F

R

V

V

G

-

Y

-

S

-

G

-

E

-

G

-

S

-

A

-

G

L

T

V

D

N

P

P

E

K

E

E

A

A

R

E

R

Q

F

F

A

V

Q

K

E

E

T

S

G

Q

V

V

D

D

A

Y

M

L

A

A

I

P

S

A

V

I

G

G

N

T

V

S

H
|
H

-

G

-

A

L

F

Q
x
K

Q

F

D

K

K

G

E

E

G

P

G

K

L

L

D

D

I

F

D

E

R

R

I

L

R

Q

A

E

I

V

E

K

A

R

I

L

T

T

E

N

V

I

P

P

L

L

V

V

I

L

H
|
H

G
|
G

G

A

S
|
S

G

A

V

I

P

P

V

D

A

N

Q

V

R

R

R

K

M

S

-

Y

-

L

-

D

-

A

-

G

-

D

-

L

-

K

-

G

-

S

-

K

-

G

-

V

-

P

-

F

-

E

L

F

A

L

R

Q

E

E

S

S

R

V

-

K

-

G

I

I

C

N

K

K

F

V

N
|
N

I

T

G
x
D

T
|
T

E

D

L

L

R
|
R

M

I

V

A

F

F

G

I

A

A

A

E

M

V

R

R

D

K

A

V

V

A

N

N

S

E

D

D

P

K

D

S

R

Q

F

F

D

D

R

-

V

-

S

-

I

-

L

L

S

R

E

K

T

F

H

F

D

S

P

P

V

A

V

Q

A

L

A

A

A

L

R

K

S

N

V

V

L

V

R

K

binding 1,6-di-O-phosphono-D-fructose: S49 (≠ G50), D82 (= D82), H83 (= H83), H165 (= H174), K169 (≠ Q176), G196 (= G203), S198 (= S205), N238 (= N225), D240 (≠ G227), T241 (= T228), R244 (= R231)
binding zinc ion: H83 (= H83), H83 (= H83), H83 (= H83), E134 (= E134), H165 (= H174), H165 (= H174), H165 (= H174), H195 (= H202), H195 (= H202)

3gb6A Structure of giardia fructose-1,6-biphosphate aldolase d83a mutant in complex with fructose-1,6-bisphosphate (see paper)
31% identity, 97% coverage: 3:273/279 of query aligns to 2:296/318 of 3gb6A

query
sites
3gb6A

I

L

A

C

T

T

L

L

A

R

E

Q

V

M

L

L

Q

G

P

E

A

A

L

R

R

K

D

H

G

K

Y

Y

A

G

V

V

A

G

G

A

L

F

V
x
N

T

V

L

N

G

N

W

M

E

E

D

Q

M

I

R

Q

A

G

Y

I

V

M

A

K

A

A

A

V

E

V

A

Q

E

L

G

K

V

S

P

P

V

V

I

I

L

L

Q

Q

A

C

G
x
S

P

R

S

G

C

A

R

L

V

K

H

Y

T

S

P

D

L

M

P

I

I

Y

L

L

G

K

K

K

M

L

F

C

R

E

H

A

-

A

L

L

A

E

E

K

G

H

A

P

S

D

V

I

P

P

V

I

V

C

A

I

H

H

L

L

D

A

H

H

G

G

Y

D

T

T

A

L

E

E

D

S

C

V

R

K

I

M

A

A

I

I

D

D

S

L

G

G

F

F

T

S

S

S

V

V

M

M

F

I

D

D

G

A

S

S

R

H

N

H

A

P

L

F

D

D

D

E

N

N

I

V

A

R

E

I

T

T

A

K

A

E

I

V

A

V

G

A

M

Y

A

A

H

H

A

A

A

R

G

G

V

V

S

S

C

V

E

E

G

A

E

E

I

L

G

G

F

T

V

L

G

G

Y

-

S

-

G

G

G

I

E

E

G

N

S

T

A

V

G

Q

-

L

T

T

D

E

P

P

E

Q

E

D

A

A

R

K

F

F

A

V

Q

E

E

L

T

T

G

G

V

V

D

D

A

A

M

L

A

A

I

V

S

A

V

I

G

G

N

T

V

S

H
|
H

-
x
G

-

A

-

Y

-
x
K

L

F

Q

K

Q

S

D

E

K

S

E

D

G

I

G

R

L

L

D

A

I

I

D

D

R

R

I

V

R

K

A

T

I

I

E

S

A

D

I

L

T

T

E

G

V

I

P

P

L

L

V

V

I

M

H
|
H

G
|
G

G

S

S
|
S

G

S

V

V

P

P

V

K

A

D

Q

V

R

K

R

D

M

M

L

I

A

N

R

K

-

Y

-

G

-

K

-

M

-

P

-

D

-

A

-

V

-

G

-

V

-

P

-

I

E

E

S

S

R

I

-

V

-

H

-

A

-

I

-

G

-

E

-

G

I

V

C

C

K

K

F

I

N
|
N

I

V

G
x
D

T
x
S

E

D

L

S

R
|
R

M

M

V

A

F

M

G

T

A

G

A

A

M

I

R

R

D

K

A

V

V

F

N

V

S

E

D

H

P

P

D

E

R

K

F

F

D

D

R

P

V

R

S

D

I

Y

L

L

S

G

E

P

T

G

H

R

D

D

P

A

V

I

V

T

A

E

A

M

A

L

R

I

S

P

V

K

L

I

R

K

A

A

F

F

binding 1,6-di-O-phosphono-D-fructose: N23 (≠ V24), S49 (≠ G50), H173 (= H174), G174 (vs. gap), K177 (vs. gap), H205 (= H202), G206 (= G203), S208 (= S205), N248 (= N225), D250 (≠ G227), S251 (≠ T228), R254 (= R231)

3n9rA Class ii fructose-1,6-bisphosphate aldolase from helicobacter pylori in complex with n-(4-hydroxybutyl)-phosphoglycolohydroxamic acid, a competitive inhibitor (see paper)
31% identity, 95% coverage: 8:271/279 of query aligns to 7:285/297 of 3n9rA

query
sites
3n9rA

E

E

V

I

L

L

Q

L

P

K

A

A

L

H

R

K

D

E

G

G

Y

Y

A

G

V

V

A

G

G

A

L

F

V

N

T

F

L

V

G

N

W

F

E

E

D

M

M

L

R

N

A

A

Y

I

V

F

A

E

A

A

A

G

E

N

A

E

E

E

G

N

V

S

P

P

V

L

I

F

L

I

Q

Q

A

A

G

S

P

E

S

G

C

A

R

I

V

K

H

Y

T

M

P

G

L

I

P

D

I

M

L

A

G

V

K

G

M

M

F

V

R

K

H

I

L

M

A
x
C

E
|
E

-

R

G

Y

A

P

S

H

V

I

P

P

V

V

V

A

A

L

H

H

L

L

D

D

H
|
H

G

G

Y

T

T

T

A

F

E

E

D

S

C

C

R

E

I

K

A

A

I

V

D

K

S

A

G

G

F

F

T

T

S

S

V

V

M

M

F

I

D

D

G

A

S

S

R

H

N

H

A

A

L

F

D

E

N

N

I

L

A

E

E

L

T

T

A

S

A

K

I

V

A

V

G

K

M

M

A

A

H

H

A

N

A

A

G

G

V

V

S

S

C

V

E

E

G

A

E

E

I

L

G
|
G

-

R

F

L

V

M

G

G

Y

I

S

-

G

-

E

-

G

-

S

A

A

V

G

L

T

V

D

N

P

P

E

K

E

E

A

A

R

E

R

Q

F

F

A

V

Q

K

E

E

T

S

G

Q

V

V

D

D

A

Y

M

L

A

A

I

P

S

A

V

I

G

G

N

T

V

S

H
|
H

-
x
G

-

A

L

F

Q
x
K

Q

F

D

K

K

G

E

E

G

P

G

K

L

L

D

D

I

F

D

E

R

R

I

L

R

Q

A

E

I

V

E

K

A

R

I

L

T

T

E

N

V

I

P

P

L

L

V

V

I

L

H
|
H

G
|
G

G

A

S
|
S

G

A

V

I

P

P

V

D

A

N

Q

V

R

R

R

K

M

S

-

Y

-

L

-

D

-
x
A

-

G

-

D

-

L

-

K

-

G

-

S

-

K

-

G

-

V

-

P

-

F

-

E

L

F

A

L

R

Q

E

E

S

S

R

V

-

K

-

G

I

I

C

N

K

K

F

V

N
|
N

I

T

G
x
D

T
|
T

E

D

L

L

R

R

M

I

V

A

F

F

G

I

A

A

A

E

M

V

R

R

D

K

A

V

V

A

N

N

S

E

D

D

P

K

D

S

R

Q

F

F

D

D

R

-

V

-

S

-

I

-

L

L

S

R

E

K

T

F

H

F

D

S

P

P

V

A

V

Q

A

L

A

A

A

L

R

K

S

N

V

V

L

V

R

K

active site: C69 (≠ A70), E70 (= E71), G136 (= G136), H170 (= H174), A216 (vs. gap), N243 (= N225)
binding 2-[hydroxy(4-hydroxybutyl)amino]-2-oxoethyl dihydrogen phosphate: H83 (= H83), H170 (= H174), G171 (vs. gap), K174 (≠ Q176), H200 (= H202), G201 (= G203), S203 (= S205), N243 (= N225), D245 (≠ G227), T246 (= T228)
binding zinc ion: H83 (= H83), H170 (= H174), H200 (= H202)

3c56A Class ii fructose-1,6-bisphosphate aldolase from helicobacter pylori in complex with n-(3-hydroxypropyl)-glycolohydroxamic acid bisphosphate, a competitive inhibitor (see paper)
31% identity, 95% coverage: 8:271/279 of query aligns to 7:285/297 of 3c56A

query
sites
3c56A

E

E

V

I

L

L

Q

L

P

K

A

A

L

H

R

K

D

E

G

G

Y

Y

A

G

V

V

A

G

G

A

L

F

V
x
N

T

F

L

V

G

N

W

F

E

E

D

M

M

L

R

N

A

A

Y

I

V

F

A

E

A

A

A

G

E

N

A

E

E

E

G

N

V

S

P

P

V

L

I

F

L

I

Q

Q

A

A

G
x
S

P

E

S

G

C

A

R

I

V

K

H

Y

T

M

P

G

L

I

P

D

I

M

L

A

G

V

K

G

M

M

F

V

R

K

H

I

L

M

A
x
C

E
|
E

-

R

G

Y

A

P

S

H

V

I

P

P

V

V

V

A

A

L

H

H

L

L

D
|
D

H
|
H

G

G

Y

T

T

T

A

F

E

E

D

S

C

C

R

E

I

K

A

A

I

V

D

K

S

A

G

G

F

F

T

T

S

S

V

V

M

M

F

I

D

D

G

A

S

S

R

H

N

H

A

A

L

F

D

E

N

N

I

L

A

E

E

L

T

T

A

S

A

K

I

V

A

V

G

K

M

M

A

A

H

H

A

N

A

A

G

G

V

V

S

S

C

V

E

E

G

A

E

E

I

L

G
|
G

-

R

F

L

V

M

G

G

Y

I

S

-

G

-

E

-

G

-

S

A

A

V

G

L

T

V

D

N

P

P

E

K

E

E

A

A

R

E

R

Q

F

F

A

V

Q

K

E

E

T

S

G

Q

V

V

D

D

A

Y

M

L

A

A

I

P

S

A

V

I

G

G

N

T

V

S

H
|
H

-

G

-

A

L

F

Q
x
K

Q

F

D

K

K

G

E

E

G

P

G

K

L

L

D

D

I

F

D

E

R

R

I

L

R

Q

A

E

I

V

E

K

A

R

I

L

T

T

E

N

V

I

P

P

L

L

V

V

I

L

H
|
H

G
|
G

G

A

S
|
S

G

A

V

I

P

P

V

D

A

N

Q

V

R

R

R

K

M

S

-

Y

-

L

-

D

-
x
A

-

G

-

D

-

L

-

K

-

G

-

S

-

K

-

G

-

V

-

P

-

F

-

E

L

F

A

L

R

Q

E

E

S

S

R

V

-

K

-

G

I

I

C

N

K

K

F

V

N
|
N

I

T

G
x
D

T
|
T

E

D

L

L

R
|
R

M

I

V

A

F

F

G

I

A

A

A

E

M

V

R

R

D

K

A

V

V

A

N

N

S

E

D

D

P

K

D

S

R

Q

F

F

D

D

R

-

V

-

S

-

I

-

L

L

S

R

E

K

T

F

H

F

D

S

P

P

V

A

V

Q

A

L

A

A

A

L

R

K

S

N

V

V

L

V

R

K

active site: C69 (≠ A70), E70 (= E71), G136 (= G136), H170 (= H174), A216 (vs. gap), N243 (= N225)
binding 3-{hydroxy[(phosphonooxy)acetyl]amino}propyl dihydrogen phosphate: N23 (≠ V24), S49 (≠ G50), D82 (= D82), H170 (= H174), K174 (≠ Q176), G201 (= G203), S203 (= S205), N243 (= N225), D245 (≠ G227), T246 (= T228), R249 (= R231)
binding zinc ion: H83 (= H83), H170 (= H174), H200 (= H202)

3n9sA Class ii fructose-1,6-bisphosphate aldolase from helicobacter pylori in complex with n-(4-hydroxybutyl)- glycolohydroxamic acid bis- phosphate, a competitive inhibitor (see paper)
31% identity, 95% coverage: 8:271/279 of query aligns to 7:295/307 of 3n9sA

query
sites
3n9sA

E

E

V

I

L

L

Q

L

P

K

A

A

L

H

R

K

D

E

G

G

Y

Y

A

G

V

V

A

G

G

A

L

F

V
x
N

T

F

L

V

G

N

W

F

E

E

D

M

M

L

R

N

A

A

Y

I

V

F

A

E

A

A

A

G

E

N

A

E

E

E

G

N

V

S

P

P

V

L

I

F

L

I

Q

Q

A

A

G
x
S

P

E

S

G

C

A

R

I

V

K

H

Y

T

M

P

G

L

I

P

D

I

M

L

A

G

V

K

G

M

M

F

V

R

K

H

I

L

M

A
x
C

E
|
E

-

R

G

Y

A

P

S

H

V

I

P

P

V

V

V

A

A

L

H

H

L

L

D
|
D

H
|
H

G

G

Y

T

T

T

A

F

E

E

D

S

C

C

R

E

I

K

A

A

I

V

D

K

S

A

G

G

F

F

T

T

S

S

V

V

M

M

F

I

D
|
D

G

A

S
|
S

R

H

N

H

A

A

L

F

D

E

N

N

I

L

A

E

E

L

T

T

A

S

A

K

I

V

A

V

G

K

M

M

A

A

H

H

A

N

A

A

G

G

V

V

S

S

C

V

E

E

G

A

E
|
E

I

L

G
|
G

F

R

V

L

G

-

Y

-

S

M

G

G

G

I

E

E

G

D

S

N

A

I

G

S

T

V

D

D

-

E

-

K

-

D

-

A

-

V

-

L

-

V

-

N

P

P

E

K

E

E

A

A

R

E

R

Q

F

F

A

V

Q

K

E

E

T

S

G

Q

V

V

D

D

A

Y

M

L

A

A

I

P

S

A

V

I

G

G

N

T

V

S

H
|
H

-
x
G

-

A

L

F

Q
x
K

Q

F

D

K

K

G

E

E

G

P

G

K

L

L

D

D

I

F

D

E

R

R

I

L

R

Q

A

E

I

V

E

K

A

R

I

L

T

T

E

N

V

I

P

P

L

L

V

V

I

L

H
|
H

G
|
G

G

A

S
|
S

G

A

V

I

P

P

V

D

A

N

Q

V

R

R

R

K

M

S

-

Y

-

L

-

D

-
x
A

-

G

-

D

-

L

-

K

-

G

-

S

-

K

-

G

-

V

-

P

-

F

-

E

L

F

A

L

R

Q

E

E

S

S

R

V

-

K

-

G

I

I

C

N

K

K

F

V

N
|
N

I

T

G
x
D

T
|
T

E

D

L

L

R

R

M

I

V

A

F

F

G

I

A

A

A

E

M

V

R

R

D

K

A

V

V

A

N

N

S

E

D

D

P

K

D

S

R

Q

F

F

D

D

R

-

V

-

S

-

I

-

L

L

S

R

E

K

T

F

H

F

D

S

P

P

V

A

V

Q

A

L

A

A

A

L

R

K

S

N

V

V

L

V

R

K

active site: C69 (≠ A70), E70 (= E71), G136 (= G136), H180 (= H174), A226 (vs. gap), N253 (= N225)
binding calcium ion: D104 (= D104), S106 (= S106), E134 (= E134)
binding 4-{hydroxy[(phosphonooxy)acetyl]amino}butyl dihydrogen phosphate: N23 (≠ V24), S49 (≠ G50), D82 (= D82), H83 (= H83), H180 (= H174), G181 (vs. gap), K184 (≠ Q176), H210 (= H202), G211 (= G203), S213 (= S205), N253 (= N225), D255 (≠ G227), T256 (= T228)
binding zinc ion: H83 (= H83), H180 (= H174), H210 (= H202)

4to8A Methicillin-resistant staphylococcus aureus class iib fructose 1,6- bisphosphate aldolase (see paper)
32% identity, 97% coverage: 3:273/279 of query aligns to 2:271/279 of 4to8A

query
sites
4to8A

I

L

A

V

T

S

L

M

A

K

E

E

V

M

L

L

Q

I

P

D

A

A

L

K

R

E

D

N

G

G

Y

Y

A

A

V

V

A

G

G

Q

L

Y

V

N

T

I

L

N

G

N

W

L

E

E

D

F

M

T

R

Q

A

A

Y

I

V

L

A

E

A

A

A

S

E

Q

A

E

E

E

G

N

V

A

P

P

V

V

I

I

L

L

Q

G

A

V

G

S

P

E

-

G

S

A

C

A

R

R

V

Y

H

M

T

S

P

G

L

F

P

Y

I

T

L

I

G

V

K

K

M

M

F

V

R

E

H

G

L

L

A

M

E

H

-

D

-

L

G

N

A

I

S

T

V

I

P

P

V

V

V

A

A

I

H

H

L

L

D
|
D

H
|
H

G

G

Y

S

T

S

A

F

E

E

D

K

C

C

R

K

I

E

A

A

I

I

D

D

S

A

G

G

F

F

T

T

S

S

V

V

M

M

F

I

D

D

G

A

S

S

R

H

N

S

A

P

L

F

D

E

N

N

I

V

A

A

E

T

T

T

A

K

I

V

A

V

G

E

M

Y

A

A

H

H

A

E

A

K

G

G

V

V

S

S

C

V

E

E

G

A

E

E

I

L

G

G

F

T

V

V

G

G

Y

G

S

D

G

D

-

V

G

A

E

D

G

G

S

I

A

I

G

Y

T

A

D

D

P

P

E

K

E

E

A

C

R

Q

R

E

F

L

A

V

Q

E

E

K

T

T

G

G

V

I

D

D

A

A

M

L

A

A

I

P

S

A

V

L

G

G

N

S

V

V

H
|
H

L

G

Q

P

Q

K

D

-

K

-

E

-

G

-

L

L

D

G

I

F

D

K

R

E

I

M

R

E

A

E

I

I

E

G

A

L

I

S

T

T

E

G

V

L

P

P

L

L

V

V

I

L

H
|
H

G

G

S

T

G

G

V

I

P

P

V

T

A

K

Q

D

R

I

R

Q

M

K

L

-

A

A

R

I

E

P

S

F

R

G

I

T

C

A

K

K

F

I

N
|
N

I

V

G

N

T

T

E

E

L

N

R

Q

M

I

V

A

F

S

G

A

A

K

A

A

M

V

R

R

D

D

A

V

V

L

N

N

S

N

D

D

P

K

D

E

R

V

F

Y

D

D

R

P

V

R

S

K

I

Y

L

L

S

G

E

P

T

A

H

R

D

E

P

A

V

I

V

K

A

E

A

T

A

V

R

K

S

G

V

K

L

I

R

K

A

E

F

F

active site: D84 (= D82), H85 (= H83), H178 (= H174), H201 (= H202), N223 (= N225)
binding zinc ion: H85 (= H83), H201 (= H202)

5ud4A Class ii fructose-1,6-bisphosphate aldolase h180q variant of helicobacter pylori with tbp (see paper)
31% identity, 95% coverage: 8:271/279 of query aligns to 7:281/293 of 5ud4A

query
sites
5ud4A

E

E

V

I

L

L

Q

L

P

K

A

A

L

H

R

K

D

E

G

G

Y

Y

A

G

V

V

A

G

G

A

L

F

V

N

T

F

L

V

G

N

W

F

E

E

D

M

M

L

R

N

A

A

Y

I

V

F

A

E

A

A

A

G

E

N

A

E

E

E

G

N

V

S

P

P

V

L

I

F

L

I

Q

Q

A

A

G
x
S

P

E

S

G

C

A

R

I

V

K

H

Y

T

M

P

G

L

I

P

D

I

M

L

A

G

V

K

G

M

M

F

V

R

K

H

I

L

M

A

C

E

E

-

R

G

Y

A

P

S

H

V

I

P

P

V

V

V

A

A

L

H

H

L

L

D
|
D

H
|
H

G

G

Y

T

T

T

A

F

E

E

D

S

C

C

R

E

I

K

A

A

I

V

D

K

S

A

G

G

F

F

T

T

S

S

V

V

M

M

F

I

D

D

G

A

S

S

R

H

N

H

A

A

L

F

D

E

N

N

I

L

A

E

E

L

T

T

A

S

A

K

I

V

A

V

G

K

M

M

A

A

H

H

A

N

A

A

G

G

V

V

S

S

C

V

E

E

G

A

E
|
E

I

L

G

G

F

R

V

L

G

-

Y

-

S

-

G

-

E

-

G

-

S

-

A

V

G

L

T

V

D

N

P

P

E

K

E

E

A

A

R

E

R

Q

F

F

A

V

Q

K

E

E

T

S

G

Q

V

V

D

D

A

Y

M

L

A

A

I

P

S

A

V

I

G

G

N

T

V

S

H
x
Q

-
x
G

-

A

L

F

Q
x
K

Q

F

D

K

K

G

E

E

G

P

G

K

L

L

D

D

I

F

D

E

R

R

I

L

R

Q

A

E

I

V

E

K

A

R

I

L

T

T

E

N

V

I

P

P

L

L

V

V

I

L

H
|
H

G
|
G

G

A

S
|
S

G

A

V

I

P

P

V

D

A

N

Q

V

R

R

R

K

M

S

-

Y

-

L

-

D

-

A

-

G

-

D

-

L

-

K

-

G

-

S

-

K

-

G

-

V

-

P

-

F

-

E

L

F

A

L

R

Q

E

E

S

S

R

V

-

K

-

G

I

I

C

N

K

K

F

V

N
|
N

I

T

G
x
D

T
|
T

E

D

L

L

R
|
R

M

I

V

A

F

F

G

I

A

A

A

E

M

V

R

R

D

K

A

V

V

A

N

N

S

E

D

D

P

K

D

S

R

Q

F

F

D

D

R

-

V

-

S

-

I

-

L

L

S

R

E

K

T

F

H

F

D

S

P

P

V

A

V

Q

A

L

A

A

A

L

R

K

S

N

V

V

L

V

R

K

binding 1,6-di-O-phosphono-D-tagatose: S49 (≠ G50), D82 (= D82), Q166 (≠ H174), G167 (vs. gap), K170 (≠ Q176), G197 (= G203), S199 (= S205), N239 (= N225), D241 (≠ G227), T242 (= T228), R245 (= R231)
binding zinc ion: H83 (= H83), H83 (= H83), E134 (= E134), H196 (= H202), H196 (= H202)

2isvA Structure of giardia fructose-1,6-biphosphate aldolase in complex with phosphoglycolohydroxamate (see paper)
30% identity, 97% coverage: 3:273/279 of query aligns to 2:277/298 of 2isvA

query
sites
2isvA

I

L

A

C

T

T

L

L

A

R

E

Q

V

M

L

L

Q

G

P

E

A

A

L

R

R

K

D

H

G

K

Y

Y

A

G

V

V

A

G

G

A

L

F

V

N

T

V

L

N

G

N

W

M

E

E

D

Q

M

I

R

Q

A

G

Y

I

V

M

A

K

A

A

A

V

E

V

A

Q

E

L

G

K

V

S

P

P

V

V

I

I

L

L

Q

Q

A

C

G

S

P

R

S

G

C

A

R

L

V

K

H

Y

T

S

P

D

L

M

P

I

I

Y

L

L

G

K

K

K

M

L

F

C

R

E

H

A

-

A

L

L

A

E

E

K

G

H

A

P

S

D

V

I

P

P

V

I

V

C

A

I

H

H

L

L

D
|
D

H
|
H

G

G

Y

D

T

T

A

L

E

E

D

S

C

V

R

K

I

M

A

A

I

I

D

D

S

L

G

G

F

F

T

S

S

S

V

V

M

M

F

I

D

D

G

A

S

S

R

H

N

H

A

P

L

F

D

D

D

E

N

N

I

V

A

R

E

I

T

T

A

K

A

E

I

V

A

V

G

A

M

Y

A

A

H

H

A

A

A

R

G

S

V

V

S

S

C

V

E

E

G

A

E

E

I

L

G

G

F

L

V

-

G

-

Y

-

S

-

G

-

E

-

G

-

S

-

A

-

G

-

T

T

D

E

P

P

E

Q

E

D

A

A

R

K

F

F

A

V

Q

E

E

L

T

T

G

G

V

V

D

D

A

A

M

L

A

A

I

V

S

A

V

I

G

G

N

T

V

S

H
|
H

L

G

Q

A

Q

R

D

-

K

-

E

-

G

-

L

L

D

A

I

I

D

D

R

R

I

V

R

K

A

T

I

I

E

S

A

D

I

L

T

T

E

G

V

I

P

P

L

L

V

V

I

M

H
|
H

G
|
G

G

S

S
|
S

G

S

V

V

P

P

V

K

A

D

Q

V

R

K

R

D

M

M

L

I

A

N

R

K

-

Y

-

G

-

K

-

M

-

P

-

D

-

A

-

V

-

G

-

V

-

P

-

I

E

E

S

S

R

I

-

V

-

H

-

A

-

I

-

G

-

E

-

G

I

V

C

C

K

K

F

I

N
|
N

I

V

G
x
D

T
x
S

E

D

L

S

R

R

M

M

V

A

F

M

G

T

A

G

A

A

M

I

R

R

D

K

A

V

V

F

N

V

S

E

D

H

P

P

D

E

R

K

F

F

D

D

R

P

V

R

S

D

I

Y

L

L

S

G

E

P

T

G

H

R

D

D

P

A

V

I

V

T

A

E

A

M

A

L

R

I

S

P

V

K

L

I

R

K

A

A

F

F

binding phosphoglycolohydroxamic acid: D82 (= D82), H163 (= H174), H186 (= H202), G187 (= G203), S189 (= S205), N229 (= N225), D231 (≠ G227), S232 (≠ T228)
binding zinc ion: H83 (= H83), H163 (= H174), H186 (= H202)

2isvB Structure of giardia fructose-1,6-biphosphate aldolase in complex with phosphoglycolohydroxamate (see paper)
30% identity, 97% coverage: 3:273/279 of query aligns to 2:286/307 of 2isvB

query
sites
2isvB

I

L

A

C

T

T

L

L

A

R

E

Q

V

M

L

L

Q

G

P

E

A

A

L

R

R

K

D

H

G

K

Y

Y

A

G

V

V

A

G

G

A

L

F

V

N

T

V

L

N

G

N

W

M

E

E

D

Q

M

I

R

Q

A

G

Y

I

V

M

A

K

A

A

A

V

E

V

A

Q

E

L

G

K

V

S

P

P

V

V

I

I

L

L

Q

Q

A

C

G

S

P

R

S

G

C

A

R

L

V

K

H

Y

T

S

P

D

L

M

P

I

I

Y

L

L

G

K

K

K

M

L

F

C

R

E

H

A

-

A

L

L

A

E

E

K

G

H

A

P

S

D

V

I

P

P

V

I

V

C

A

I

H

H

L

L

D
|
D

H
|
H

G

G

Y

D

T

T

A

L

E

E

D

S

C

V

R

K

I

M

A

A

I

I

D

D

S

L

G

G

F

F

T

S

S

S

V

V

M

M

F

I

D

D

G

A

S

S

R

H

N

H

A

P

L

F

D

D

D

E

N

N

I

V

A

R

E

I

T

T

A

K

A

E

I

V

A

V

G

A

M

Y

A

A

H

H

A

A

A

R

G

S

V

V

S

S

C

V

E

E

G

A

E

E

I

L

G

-

F

-

V

-

G

-

Y

-

S

-

G

G

G

Q

E

N

G

T

S

V

A

Q

G

L

T

T

D

E

P

P

E

Q

E

D

A

A

R

K

F

F

A

V

Q

E

E

L

T

T

G

G

V

V

D

D

A

A

M

L

A

A

I

V

S

A

V

I

G

G

N

T

V

S

H
|
H

L

-

Q
x
G

Q

A

D

Y

K
|
K

E

F

G

I

G

R

L

L

D

A

I

I

D

D

R

R

I

V

R

K

A

T

I

I

E

S

A

D

I

L

T

T

E

G

V

I

P

P

L

L

V

V

I

M

H
|
H

G
|
G

G

S

S
|
S

G

S

V

V

P

P

V

K

A

D

Q

V

R

K

R

D

M

M

L

I

A

N

R

K

-

Y

-

G

-

K

-

M

-

P

-

D

-

A

-

V

-

G

-

V

-

P

-

I

E

E

S

S

R

I

-

V

-

H

-

A

-

I

-

G

-

E

-

G

I

V

C

C

K

K

F

I

N
|
N

I

V

G
x
D

T
x
S

E

D

L

S

R

R

M

M

V

A

F

M

G

T

A

G

A

A

M

I

R

R

D

K

A

V

V

F

N

V

S

E

D

H

P

P

D

E

R

K

F

F

D

D

R

P

V

R

S

D

I

Y

L

L

S

G

E

P

T

G

H

R

D

D

P

A

V

I

V

T

A

E

A

M

A

L

R

I

S

P

V

K

L

I

R

K

A

A

F

F

binding phosphoglycolohydroxamic acid: D82 (= D82), H168 (= H174), G169 (≠ Q176), K172 (= K179), H195 (= H202), G196 (= G203), S198 (= S205), N238 (= N225), D240 (≠ G227), S241 (≠ T228)
binding zinc ion: H83 (= H83), H168 (= H174), H195 (= H202)

1rv8B Class ii fructose-1,6-bisphosphate aldolase from thermus aquaticus in complex with cobalt (see paper)
33% identity, 97% coverage: 3:273/279 of query aligns to 2:299/305 of 1rv8B

query
sites
1rv8B

I

L

A

V

T

T

L

G

A

L

E

E

V

I

L

L

Q

K

P

K

A

A

L

R

R

E

D

E

G

G

Y

Y

A

G

V

V

A

G

G

A

L

F

V

N

T

V

L

N

G

N

W

M

E

E

D

F

M

L

R

Q

A

A

Y

V

V

L

A

E

A

A

E

E

A

E

E

Q

G

R

V

S

P

P

V

V

I

I

L

L

Q

A

A

L

G

S

P

E

S

G

C

A

R

M

V

K

H

Y

T

G

P

G

L

R

P

A

I

-

L

L

G

T

K

L

M

M

F

A

R

V

H

E

L

L

A

A

E

K

G

E

A

A

S

R

V

V

P

P

V

V

V

A

A

V

H

H

L

L

D
|
D

H
|
H

G

G

Y

S

T

S

A

Y

E

E

D

S

C

V

R

L

I

R

A

A

I

L

D

R

S

A

G

G

F

F

T

T

S

S

V

V

M

M

F

I

D

D

G

K

S

S

R

H

N

E

A

D

L

F

D

E

D

T

N

N

I

V

A

R

E

E

T

T

A
x
R

A

R

I

V

A

V

G

E

M

A

A

A

H
|
H

A

A

A

V

G

G

V

V

S

T

C

V

E

E

G

A

E
|
E

I

L

G

G

F

R

V

L

G

-

Y

-

S

A

G

G

G

I

E
|
E

G

E

S

H

A

V

G

A

-

V

-

D

-

E

-

K

-

D

-

A

-

L

T

T

D

N

P

P

E

E

A

A

R

R

R

I

F

F

A

M

Q

E

E

R

T

T

G

G

V

A

D

D

A

Y

M

L

A

A

I

V

S

A

V

I

G

G

N

T

V

S

H
|
H

L

G

Q

A

Q

Y

D

K

K

G

E

K

G

G

G

R

L

P

D

F

I

I

D

D

-

H

-

A

R

R

I

L

R

E

A

R

I

I

E

A

A

R

I

L

T

V

E

P

V

A

P

P

L

L

V

V

I

L

H
|
H

G

G

G

A

S
|
S

G

A

V

V

P

P

-

P

-

E

-

L

V

V

A

E

Q

R

R

F

R

R

M

A

L

S

A

G

R

G

E

E

-

I

-

G

-

E

-

A

-

G

-

I

-

H

-

P

-

E

-

D

-

I

-

K

-

A

-

I

-

S

S

L

R

G

I

I

C

A

K

K

F

I

N
|
N

I

T

G
x
D

T
|
T

E

D

L

L

R

R

M

L

V

A

F

F

G

T

A

A

A

L

M

I

R

R

D

E

A

A

V

L

N

N

S

K

D

N

P

P

D

K

R

E

F

F

D

D

R

P

V

R

S

K

I

Y

L

L

S

G

E

P

T

A

H

R

D

E

P

A

V

V

V

K

A

E

A

V

R

K

S

S

V

R

L

M

R

E

A

L

F

F

active site: D80 (= D82), H81 (= H83), E140 (= E144), H178 (= H174), H208 (= H202), N251 (= N225)
binding cobalt (ii) ion: H81 (= H83), E132 (= E134), H178 (= H174), H208 (= H202)
binding sulfate ion: R116 (≠ A118), H123 (= H125), S211 (= S205), D253 (≠ G227), T254 (= T228)

Query Sequence

>GFF707 PGA1_c07220 putative 6-phospho-5-dehydro-2-deoxy-D-gluconate aldolase
MTIATLAEVLQPALRDGYAVAGLVTLGWEDMRAYVAAAEAEGVPVILQAGPSCRVHTPLP
ILGKMFRHLAEGASVPVVAHLDHGYTAEDCRIAIDSGFTSVMFDGSRNALDDNIAETAAI
AGMAHAAGVSCEGEIGFVGYSGGEGSAGTDPEEARRFAQETGVDAMAISVGNVHLQQDKE
GGLDIDRIRAIEAITEVPLVIHGGSGVPVAQRRMLARESRICKFNIGTELRMVFGAAMRD
AVNSDPDRFDRVSILSETHDPVVAAARSVLRAFKGETQC

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory