SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF792 FitnessBrowser__WCS417:GFF792 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 2 hits to proteins with known functional sites (download)

A0A0H2ZQB9 Ergothioneine transporter EgtUBC from Streptococcus pneumoniae serotype 2 (strain D39 / NCTC 7466) (see paper)
41% identity, 64% coverage: 76:228/238 of query aligns to 51:203/506 of A0A0H2ZQB9

query
sites
A0A0H2ZQB9

A

A

E

D

R

W

F

V

M

L

Q

Q

I

I

F

A

N

G

I

I

G

F

N

Q

T

T

V

I

P

P

P

S

L

L

A

A

V

L

L

L

A

G

I

L

A

F

L

I

G

P

V

L

L

M

G

G

I

I

G

G

S

T

G

L

P

P

A

A

I

L

F

T

A

A

L

L

F

V

L

I

A

Y

S

A

L

I

L

F

P

P

I

I

V

L

R

Q

N

N

T

T

Y

I

E

T

G

G

L

L

K

K

N

G

V

I

Q

D

G

P

S

S

L

L

K

Q

E

E

A

A

A

G

V

I

G

A

I

F

G

G

M

M

T

T

P

R

R

W

Q

E

V

R

L

L

F

K

R

K

V

F

E

E

L

I

P

P

N

L

A

A

V

M

P

P

I

V

I

I

I

M

G

S

G

G

V

I

R

R

V

T

A

A

L

A

A

V

I

L

N

I

V

I

G

G

T

T

A

A

P

T

L

L

A

A

F

T

L

L

I

I

G

G

A

A

N

G

S

G

L

L

G

G

S

S

L

F

I

I

F

L

P

L

G

G

I

I

A

D

L

R

N

N

N

N

Q

A

P

S

Q

L

L

I

L

L

L

I

G

G

A

A

A

L

C

S

T

S

A

A

L

V

L

L

A

A

L

I

L

A

L

F

D

N

G

F

L

L

V

L

T

K

M

V

A

M

S

E

R

K

Sites not aligning to the query:

244 G→F: Disrupts ergothioneine (EGT) binding. Binds EGT 100-fold more weakly. May have a global impact on overall structure.
293 F→Y: Disrupts ergothioneine (EGT) binding.
375 E→Q: Disrupts ergothioneine (EGT) binding. Binds EGT 500-fold more weakly.
419 Y→F: Disrupts ergothioneine (EGT) binding.

B5Z7I3 Ergothioneine transport permease/ergothioneine binding protein EgtU from Helicobacter pylori (strain G27) (see paper)
34% identity, 82% coverage: 31:225/238 of query aligns to 43:238/553 of B5Z7I3

query
sites
B5Z7I3

I

I

E

K

L

E

Y

F

R

H

D

D

D

R

L

F

L

I

F

Y

Y

F

L

I

Q

D

-

L

-

L

-

A

A

Q

H

H

L

F

I

I

L

I

V

V

L

A

V

L

S

S

M

G

L

F

A

L

A

V

L

L

V

V

V

F

G

G

I

V

P

L

A

I

G

G

I

V

L

F

L

-

S

A

R

F

P

Y

N

N

M

S

V

K

G

A

R

R

A

A

E

-

R

F

F

L

M

L

Q

P

I

V

F

V

N

N

I

F

G

L

N

Y

T

T

V

I

P

P

P

S

L

L

A

A

V

L

L

F

A

A

I

L

A

F

L

I

G

P

V

V

L

I

G

G

I

V

G

G

S

L

G

K

P

N

A

A

I

L

F

L

A

V

L

L

F

V

L

L

A

Y

S

G

L

L

L

L

P

P

I

I

V

V

R

Y

N

S

T

T

Y

Y

E

N

G

A

L

L

K

K

N

E

V

V

Q

R

G

E

S

E

L

V

K

I

E

K

A

A

A

A

V

I

G

G

I

L

G

G

M

C

T

N

P

P

R

K

Q

E

V

L

L

F

F

F

R

R

V

V

E

H

L

F

P

L

N

L

A

A

V

I

P

P

I

Q

I

I

I

L

G

A

G

G

V

L

R

R

V

I

A

A

L

V

A

V

I

M

N

L

V

V

G

A

T

M

A

A

P

G

L

I

A

G

F

A

L

L

I

I

G

G

A

A

N

G

S

G

L

L

G

G

S

Q

L

A

I

I

F

F

P

R

G

G

I

L

A

N

L

T

N

Q

N

N

Q

T

P

T

Q

L

L

L

L

V

L

A

G

G

A

S

A

L

C

I

T

I

A

A

L

L

L

F

A

S

L

V

L

L

L

A

D

D

G

K

L

F

V

V

T

S

M

V

Sites not aligning to the query:

390 Y→A: Disrupts EGT binding.; Y→F: Retains EGT binding.
454 R→A: Retains EGT binding.; R→E: Disrupts EGT binding.

Query Sequence

>GFF792 FitnessBrowser__WCS417:GFF792
MAIRYGKGLIGGAVVVALLALLVHWIGINTIELYRDDLLFYLQAHLILVLVSMLAALVVG
IPAGILLSRPNMVGRAERFMQIFNIGNTVPPLAVLAIALGVLGIGSGPAIFALFLASLLP
IVRNTYEGLKNVQGSLKEAAVGIGMTPRQVLFRVELPNAVPIIIGGVRVALAINVGTAPL
AFLIGANSLGSLIFPGIALNNQPQLLLGAACTALLALLLDGLVTMASRLWLERGLRPS

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory