SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF814 FitnessBrowser__WCS417:GFF814 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P51174 Long-chain specific acyl-CoA dehydrogenase, mitochondrial; LCAD; EC 1.3.8.8 from Mus musculus (Mouse) (see paper)
56% identity, 99% coverage: 4:378/378 of query aligns to 50:426/430 of P51174

query
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P51174

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K318 (= K270) modified: N6-acetyllysine; mutation to R: Reduces activity by 37%; reduces activity by 80% when associated with R-322.
K322 (= K274) modified: N6-acetyllysine; alternate; mutation to R: Reduces activity by 23%; reduces activity by 80% when associated with R-318.

Sites not aligning to the query:

42 modified: N6-acetyllysine; K→R: Reduces activity by 90% when associated with R-318 and R-322.

2pg0A Crystal structure of acyl-coa dehydrogenase from geobacillus kaustophilus
56% identity, 98% coverage: 10:378/378 of query aligns to 8:378/380 of 2pg0A

query
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2pg0A

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active site: M124 (= M126), T125 (= T127), E243 (= E243), A364 (= A364), R376 (= R376)
binding flavin-adenine dinucleotide: I122 (= I124), M124 (= M126), T125 (= T127), G130 (= G132), S131 (= S133), F155 (= F157), I156 (= I158), T157 (= T159), R269 (= R269), F272 (= F272), F279 (= F279), Q337 (= Q337), L338 (= L338), G340 (= G340), G341 (= G341), V359 (= V359), I362 (= I362), Y363 (= Y363), T366 (= T366), E368 (= E368), M369 (≠ I369)

P28330 Long-chain specific acyl-CoA dehydrogenase, mitochondrial; LCAD; EC 1.3.8.8 from Homo sapiens (Human) (see 2 papers)
53% identity, 99% coverage: 4:378/378 of query aligns to 50:426/430 of P28330

query
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P28330

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E291 (= E243) active site, Proton acceptor; mutation to Q: Loss of long-chain-acyl-CoA dehydrogenase activity. No effect on protein abundance. No effect on solubility. No effect on substrate binding.
S303 (≠ A255) to T: in dbSNP:rs1801204
K333 (= K285) to Q: in dbSNP:rs2286963

5ol2F The electron transferring flavoprotein/butyryl-coa dehydrogenase complex from clostridium difficile (see paper)
41% identity, 99% coverage: 6:378/378 of query aligns to 3:376/378 of 5ol2F

query
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5ol2F

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W

Y

P

I

I

L

Y

H

Q

Y

Y

G

G

S

N

E

E

A

E

L

Q

K

K

H

Q

K

K

Y

F

L

L

P

R

K

P

L

L

I

A

S

S

G

G

E

E

M

K

V

L

T

G

A

A

I
x
F

A

G

M
x
L

T
|
T

E

E

P
|
P

G

N

A

A

G

G

S
x
T

D

D

L

A

Q

S

G

G

V

Q

K

Q

T

T

T

T

A

A

V

V

L

L

D

D

G

G

D

D

E

E

Y

Y

V

I

I

L

N

N

G

G

S

S

K

K

T

I

F
|
F

I
|
I

T
|
T

N

N

G

A

Y

I

L

A

A

G

E

D

L

I

V

Y

I

V

V

V

V

M

A

A

K

M

T

T

D

D

P

K

K

S

A

K

G

G

A

N

K

K

G

G

T

I

S

S

L

A

F

F

L

I

V

V

E

E

A

K

D

G

T

T

P

P

G

G

F

F

D

S

K

F

G

G

K

V

R

K

L
x
E

E

K

K

K

V

M

G

G

M

I

K

R

A

G

Q

S

D

A

T

T

S

S

E

E

L

L

F

I

F

F

Q

E

D

D

V

C

R

R

V

I

P

P

K

K

E

E

N

N

L

L

L

L

G

G

Q

K

A

E

G

G

M

Q

G

G

F

F

A

K

Y

I

L

A

M

M

Q

S

E

T

L
|
L

P

D

Q

G

E
x
G

R
|
R

L

I

T

G

V

I

A

A

I

A

G

Q

A

A

L

L

S

G

S

L

A

A

E

Q

A

G

A

A

L

L

E

D

W

E

T

T

L

V

E

K

Y

Y

T

V

R

K

E

E

R
|
R

K

V

A

Q

F
|
F

G

G

K

R

A

P

I
x
L

A

S

D

K

F
|
F

Q

Q

N

N

T

T

R

Q

F

F

K

Q

L

L

A

A

E

D

M

M

A

E

T

V

E

K

I

V

Q

Q

I

A

G

A

R

R

V

H

F

L

V

V

D

Y

K

Q

C

A

M

A

A

I

L

N

H

K

L

D

E

L

G

G

K

K

L

P

D

Y

V

G

P

V

T

E

A

A

A

A

M

M

A

A

K

K

Y

L

W

F

A

A

T

A

D

E

L

T

Q

A

C

M

K

E

V

V

L

T

D

T

E

K

C

A

V

V

Q
|
Q

L
|
L

H

H

G
|
G

G
|
G

Y

Y

G

G

F

Y

M

T

W

R

E

D

Y

Y

P

P

I

V

A

E

R

R

A

M

W

M

A

R

D

D

A

A

R

K

V

I

Q

T

R

E

I

I

Y
|
Y

A
x
E

G
|
G

T
|
T

N

S

E
|
E

I

V

M

Q

K

R

E

M

I

V

I

I

A

S

R
x
G

A

K

L

L

active site: L124 (≠ M126), T125 (= T127), G241 (≠ E243), G374 (≠ R376)
binding calcium ion: E29 (≠ H32), E33 (≠ Q36), R35 (≠ Y38)
binding coenzyme a persulfide: L238 (= L240), R242 (= R244), E362 (≠ A364), G363 (= G365)
binding flavin-adenine dinucleotide: F122 (≠ I124), L124 (≠ M126), T125 (= T127), P127 (= P129), T131 (≠ S133), F155 (= F157), I156 (= I158), T157 (= T159), E198 (≠ L200), R267 (= R269), F270 (= F272), L274 (≠ I276), F277 (= F279), Q335 (= Q337), L336 (= L338), G338 (= G340), G339 (= G341), Y361 (= Y363), T364 (= T366), E366 (= E368)

4l1fA Electron transferring flavoprotein of acidaminococcus fermentans: towards a mechanism of flavin-based electron bifurcation (see paper)
42% identity, 98% coverage: 7:378/378 of query aligns to 5:377/380 of 4l1fA

query
sites
4l1fA

F
x
L

S

T

P

E

E

D

H

Q

E
x
Q

L

M

F

I

R

K

E

D

S

M

V

A

R

A

T

E

F

F

L

A

E

E

K

K

D

F

A

L

A

A

P

P

F

T

H

V

G

E

H

E

W

R

E

D

K

K

Q

A

G

H

Y

I

I

W

D

D

R

R

R

K

L

L

W

I

N

D

K

K

A

M

G

G

E

E

A

A

G

G

M

F

L

C

C

G

S

I

H

C

L

F

P

P

E

E

E

E

Y

Y

G

G

G

G

L

M

G

G

A

L

D

D

F

V

L

L

Y

S

S

Y

A

I

V

L

V

A

I

V

E

E

E

E

I

L

S

S

R

K

L

V

G

D

L

D

-

G

T

T

G

G

I

I

G

T

F

L

S

S

L

A

H

N

S

V

D

S

I

L

V

C

A

A

P

T

Y

P

I

I

L

Y

H

M

Y

F

G

G

S

T

E

E

A

E

L

Q

K

K

H

Q

K

K

Y

Y

L

L

P

A

K

P

L

I

I

A

S

E

G

G

E

T

M

H

V

V

T

G

A

A

I
x
F

A

G

M
x
L

T
|
T

E

E

P

P

G

S

A

A

G
|
G

S
x
T

D

D

L

A

Q

S

G

A

V

Q

K

Q

T

T

T

T

A

A

V

V

L

L

D

K

G

G

D

D

E

K

Y

Y

V

I

I

L

N

N

G

G

S

S

K

K

T

I

F
|
F

I
|
I

T
|
T

N

N

G

G

Y

K

L

E

A

A

E

D

L

T

V

Y

I

V

V

V

V

F

A

A

K

M

T

T

D

D

P

K

K

S

A

Q

G

G

A

V

K
x
H

G

G

T

I

S

S

L

A

F

F

L

I

V

L

E

E

A

K

D

G

T

M

P

P

G

G

F

F

D

R

K

F

G

G

K

K

R

I

L

E

E

D

K

K

V

M

G

G

M

G

K

H

A

T

Q

S

D

I

T

T

S

A

E

E

L

L

F

I

F

F

Q

E

D

D

V

C

R

E

V

V

P

P

K

K

E

E

N

N

L

L

L

L

G

G

Q

K

A

E

G

G

M

E

G

G

F
|
F

A

K

Y

I

L

A

M
|
M

Q
x
E

E

T

L
|
L

P
x
D

Q

G

E
x
G

R
|
R

L

I

T

G

V

V

A

A

I

A

G

Q

A

A

L

L

S

G

S

I

A

A

E

E

A

G

A

A

L

L

E

A

W

A

T

A

L

V

E

K

Y

Y

T

S

R

K

E

E

R
|
R

K

E

A
x
Q

F
|
F

G

G

K

R

A

S

I
|
I

A

S

D

K

F
|
F

Q

Q

N

A

T
x
L

R

Q

F

F

K

M

L

M

A

A

E

D

M

M

A

A

T

T

E

K

I

I

Q

E

I

A

G

A

R

R

V

Y

F

L

V

V

D

Y

K

H

C

A

M

A

A

M

L

L

H

K

L

N

E

E

G

G

K

K

L

P

D

Y

V

S

P

E

T

A

A

A

A

A

M

M

A

A

K

K

Y

C

W

F

A

A

T

S

D

D

L

V

Q

A

C

M

K

E

V

V

L

T

D

T

E

D

C

A

V

V

Q
|
Q

L
x
I

H

F

G

G

G
|
G

Y

Y

G

G

F

Y

M

T

W

V

E

D

Y

Y

P

P

I

A

A

E

R

R

A

Y

W

M

A

R

D

N

A

A

R

K

V
x
I

Q

T

R

Q

I

I

Y
|
Y

A
x
E

G
|
G

T
|
T

N

N

E
x
Q

I

V

M

M

K

R

E

I

I

V

I

T

A

S

R
|
R

A

A

L

L

active site: L125 (≠ M126), T126 (= T127), G242 (≠ E243), E363 (≠ A364), R375 (= R376)
binding coenzyme a persulfide: T132 (≠ S133), H179 (≠ K180), F232 (= F233), M236 (= M237), E237 (≠ Q238), L239 (= L240), D240 (≠ P241), R243 (= R244), Y362 (= Y363), E363 (≠ A364), G364 (= G365), R375 (= R376)
binding flavin-adenine dinucleotide: F123 (≠ I124), L125 (≠ M126), T126 (= T127), G131 (= G132), T132 (≠ S133), F156 (= F157), I157 (= I158), T158 (= T159), R268 (= R269), Q270 (≠ A271), F271 (= F272), I275 (= I276), F278 (= F279), L281 (≠ T282), Q336 (= Q337), I337 (≠ L338), G340 (= G341), I358 (≠ V359), Y362 (= Y363), T365 (= T366), Q367 (≠ E368)
binding 1,3-propandiol: L5 (≠ F7), Q10 (≠ E12)

5lnxD Crystal structure of mmgc, an acyl-coa dehydrogenase from bacillus subtilis.
42% identity, 98% coverage: 10:378/378 of query aligns to 5:372/374 of 5lnxD

query
sites
5lnxD

E

E

H

Q

E

V

L

M

F

M

R

R

E

K

S

M

V

V

R

R

T

D

F

F

L

A

E

R

K

K

D

E

A

I

A

A

P

P

F

A

H

A

G

E

H

I

W

M

E

E

K

K

Q

T

G

D

Y

E

I

F

D

P

R

F

R

Q

L

L

W

I

N

K

K

K

A

M

G

G

E

K

A

H

G

G

M

L

L

M

C

G

S

I

H

P

L

V

P

P

E

E

E

Q

Y

Y

G

G

G

G

L

A

G

G

A

A

D

D

F

V

L

V

Y

S

S

Y

A

I

V

L

V

A

I

I

E

H

E

E

I

I

S

S

R

R

L

I

-

S

G

A

L

A

T

V

G

G

I

V

G

I

F

L

S

S

L

V

H

H

S

T

D

S

I

V

V

G

A

T

P

N

Y

P

I

I

L

L

H

Y

Y

F

G

G

S

N

E

E

A

E

L

Q

K

K

H

M

K

K

Y

Y

L

I

P

P

K

N

L

L

I

A

S

S

G

G

E

D

M

H

V

L

T

G

A

A

I

F

A

A

M
x
L

T
|
T

E

E

P

P

G

H

A

S

G
|
G

S
|
S

D

D

L

A

Q

G

G

S

V

L

K

R

T

T

T

T

A

A

V

I

L

K

D

K

G

N

D

G

E

K

Y

Y

V

L

I

L

N

N

G

G

S

S

K

K

T

I

F
|
F

I

I

T
|
T

N

N

G

G

Y

G

L

A

A

A

E

D

L

I

V

Y

I

I

V

T

V

F

A

A

K

L

T

T

D

A

P

P

K

D

A

Q

G

G

A

R

K

H

G

G

T

I

S

S

L

A

F

F

L

I

V

V

E

E

A

K

D

N

T

T

P

P

G

G

F

F

D

T

K

V

G

G

K

K

R

K

L

E

E

R

K

K

V

L

G

G

M

L

K

Y

A

G

Q

S

D

N

T

T

S

T

E

E

L

L

F

I

F

F

Q

D

D

N

V

A

R

E

V

V

P

P

K

E

E

A

N

N

L

L

L

L

G

G

Q

K

A

E

G

G

M

D

G

G

F

F

A

H

Y

I

L

A

M

M

Q

A

E

N

L

L

P

N

Q

V

E
x
G

R

R

L

I

T

G

V

I

A

A

I

A

G

Q

A

A

L

L

S

G

S

I

A

A

E

E

A

A

A

A

L

L

E

E

W

H

T

A

L

V

E

D

Y

Y

T

A

R

K

E

Q

R
|
R

K

V

A
x
Q

F
|
F

G

G

K

R

A

P

I
|
I

A

A

D

A

F
x
N

Q

Q

N

G

T
x
I

R

S

F

F

K

K

L

L

A

A

E

D

M

M

A

A

T

T

E

R

I

A

Q

E

I

A

G

A

R

R

V

H

F

L

V

V

D

Y

K

H

C

A

M

A

A

D

L

L

H

H

L

-

E

-

G

N

K

R

L

L

D

N

V

C

-

G

P

K

T

E

A

A

A

S

M

M

A

A

K

K

Y

Q

W

F

A

A

T

S

D

D

L

A

Q

A

C

V

K

K

V

A

L

L

D

D

E

-

C

A

V

V

Q
|
Q

L
x
I

H

Y

G

G

G
|
G

Y

Y

G

G

F

Y

M

M

W

K

E

D

Y

Y

P

P

I

V

A

E

R

R

A

L

W

L

A

R

D

D

A

A

R

K

V

V

Q

T

R

Q

I

I

Y
|
Y

A
x
E

G

G

T
|
T

N

N

E
|
E

I

I

M

Q

K

R

E

L

I

I

I

I

A

S

R
x
K

A

Y

L

L

active site: L122 (≠ M126), T123 (= T127), G239 (≠ E243), E358 (≠ A364), K370 (≠ R376)
binding flavin-adenine dinucleotide: L122 (≠ M126), T123 (= T127), G128 (= G132), S129 (= S133), F153 (= F157), T155 (= T159), R265 (= R269), Q267 (≠ A271), F268 (= F272), I272 (= I276), N275 (≠ F279), I278 (≠ T282), Q331 (= Q337), I332 (≠ L338), G335 (= G341), Y357 (= Y363), T360 (= T366), E362 (= E368)

3r7kA Crystal structure of a probable acyl coa dehydrogenase from mycobacterium abscessus atcc 19977 / dsm 44196 (see paper)
39% identity, 99% coverage: 3:378/378 of query aligns to 1:377/378 of 3r7kA

query
sites
3r7kA

P

P

R

E

T

A

L

W

F

T

S

T

P

P

E

E

H

R

E

R

L

A

F

L

R

S

E

Q

S

M

V

A

R

R

T

S

F

F

L

V

E

E

K

R

D

E

A

I

A

A

P

P

F

K

H

L

G

A

H

E

W

W

E

E

K

H

Q

V

G

G

Y

E

I

I

D

P

R

R

R

D

L

L

W

H

N

L

K

N

A

A

G

A

E

E

A

V

G

G

M

L

L

L

C

G

S

I

H

G

L

F

P

P

E

E

E

E

Y

V

G

G

G

G

L

S

G

G

A

G

D

N

F

A

L

I

Y

D

S

S

A

A

V

L

V

V

I

T

E

E

E

A

I

I

-

L

S

A

R

A

L

G

G

G

L

S

T

T

G

G

I

V

G

C

F

A

S

A

L

L

H

F

S

T

D

H

I

G

V

I

A

A

-

L

P

P

Y

H

I

I

L

A

H

A

Y

N

G

G

S

S

E

D

A

A

L

L

K

I

H

E

K

R

Y

Y

L

V

P

R

K

P

L

T

I

L

S

A

G

G

E

K

M

M

V

I

T

G

A

S

I

L

A

G

M
x
V

T
|
T

E

E

P

P

G

G

A

A

G
|
G

S
|
S

D

D

L

V

Q

A

G

N

V

L

K

R

T

T

T

R

A

A

V

V

L

R

D

E

G

G

D

D

E

T

Y

Y

V

V

I

V

N

N

G

G

S

A

K

K

T

T

F
|
F

I
|
I

T
|
T

N

S

G

G

Y

V

L

R

A

A

E

D

L

F

V

V

I

T

V

T

V

A

A

V

K

R

T

T

D

G

P

-

K

G

A

P

G

G

A

Y

K

G

G

G

T

V

S

S

L

L

F

L

L

V

V

I

E

D

A

K

D

N

T

S

P

P

G

G

F

F

D

E

K

V

G

S

K

R

R

R

L

L

E

D

K

K

V

M

G

G

M

W

K

R

A

C

Q

S

D

D

T

T

S

A

E

E

L

L

F

S

F

F

Q

V

D

D

V

V

R

R

V

V

P

P

K

A

E

D

N

N

L

L

L

V

G

G

Q

A

A

E

G

N

M

S

G

G

F

F

A

L

Y

Q

L

I

M

M

Q

Q

E

Q

L

F

P

Q

Q

A

E
|
E

R

R

L

L

T

G

V

I

A

A

I

V

G

Q

A

A

L

Y

S

A

S

T

A

A

E

G

A

R

A

A

L

L

E

D

W

L

T

A

L

K

E

S

Y

W

T

A

R

R

E

E

R
|
R

K

E

A
x
T

F
|
F

G

G

K

R

A

P

I
x
L

A

T

D

G

F
x
R

Q

Q

N

I

T
x
I

R

R

F

H

K

K

L

L

A

A

E

E

M

M

A

A

T

R

E

Q

I

V

Q

D

I

V

G

A

R

C

V

T

F

Y

V

T

D

R

K

A

C

V

M

M

A

Q

L

R

H

W

L

L

E

A

G

G

K

E

L

D

D

V

V

V

P

A

T

E

A

V

A

S

M

M

A

A

K

K

Y

N

W

T

A

A

T

V

D

Y

L

A

Q

C

C

D

K

Y

V

V

L

V

D

N

E

E

C

A

V

V

Q
|
Q

L
x
I

H

F

G

G

G
|
G

Y

M

G

G

F

Y

M

M

W

R

E

E

Y

S

P

E

I

I

A

E

R

R

A

H

W

Y

A

R

D

D

A

C

R

R

V
x
I

Q

L

R

G

I

I

Y

G

A
x
G

G

G

T
|
T

N

N

E
|
E

I

I

M

M

K

N

E

E

I

V

I

I

A

A

R
x
K

A

R

L

I

active site: V126 (≠ M126), T127 (= T127), E242 (= E243), G363 (≠ A364), K375 (≠ R376)
binding dihydroflavine-adenine dinucleotide: V126 (≠ M126), T127 (= T127), G132 (= G132), S133 (= S133), F157 (= F157), I158 (= I158), T159 (= T159), R268 (= R269), T270 (≠ A271), F271 (= F272), L275 (≠ I276), R278 (≠ F279), I281 (≠ T282), Q336 (= Q337), I337 (≠ L338), G340 (= G341), I358 (≠ V359), T365 (= T366), E367 (= E368)

3pfdC Crystal structure of an acyl-coa dehydrogenase from mycobacterium thermoresistibile bound to reduced flavin adenine dinucleotide solved by combined iodide ion sad mr (see paper)
37% identity, 97% coverage: 11:378/378 of query aligns to 1:367/369 of 3pfdC

query
sites
3pfdC

H

H

E

I

L

A

F

L

R

R

E

E

S

A

V

I

R

R

T

A

F

L

L

A

E

E

K

K

D

E

A

I

A

A

P

P

F

Y

H

A

G

A

H

E

W

V

E

D

K

E

Q

K

G

A

Y

R

I

F

D

P

R

E

R

E

L

A

W

L

N

A

K

A

A

L

G

N

E

S

A

S

G

G

M

F

L

S

C

A

S

I

H

H

L

V

P

P

E

E

E

E

Y

Y

G

G

G

G

L

Q

G

G

A

A

D

D

F

S

L

V

Y

A

S

T

A

C

V

I

V

V

I

I

E

E

E

E

I

V

S

A

R

R

L

V

G

D

L

C

T

S

G

A

I

S

G

L

F

I

S

P

L

A

H

V

S

N

D

K

I

L

V

G

A

T

P

M

Y

G

I

L

L

I

H

L

Y

R

G

G

S

S

E

E

A

E

L

L

K

K

H

K

K

Q

Y

V

L

L

P

P

K

A

L

V

I

A

S

S

G

G

E

E

M

A

V

M

T

A

A

S

I
x
Y

A

A

M
x
L

T
x
S

E

E

P

R

G

E

A

A

G
|
G

S
|
S

D

D

L

A

Q

A

G

S

V

M

K

R

T

T

T

R

A

A

V

V

L

A

D

D

G

G

D

D

E

D

Y

W

V

I

I

L

N

N

G

G

S

S

K

K

T

C

F
x
W

I
|
I

T
|
T

N

N

G

G

Y

G

L

K

A

S

E

T

L

W

V

Y

I

T

V

V

V

M

A

A

K

V

T

T

D

D

P

P

K

D

A

K

G

G

A

A

K

N

G

G

T

I

S

S

L

A

F

F

L

M

V

V

E

H

A

K

D

D

T

D

P

E

G

G

F

F

D

T

K

V

G

G

K

P

R

K

L

E

E

R

K

K

V

L

G

G

M

I

K

K

A

G

Q

S

D

P

T

T

S

T

E

E

L

L

F

Y

F

F

Q

E

D

N

V

C

R

R

V

I

P

P

K

G

E

D

N

R

L

I

L

I

G

G

Q

E

A

P

G

G

M

T

G

G

F

F

A

K

Y

T

L

A

M

L

Q

A

E

T

L

L

P

D

Q

H

E
x
T

R

R

L

P

T

T

V

I

A

G

I

A

G

Q

A

A

L

V

S

G

S

I

A

A

E

Q

A

G

A

A

L

L

E

D

W

A

T

A

L

I

E

A

Y

Y

T

T

R

K

E

E

R
|
R

K

K

A

Q

F
|
F

G

G

K

R

A

P

I
x
V

A

S

D

D

F
x
N

Q

Q

N

G

T

V

R

Q

F

F

K

M

L

L

A

A

E

D

M

M

A

A

T

M

E

K

I

I

Q

E

I

A

G

A

R

R

V

L

F

M

V

V

D

Y

K

S

C

A

M

A

A

A

L

R

H

A

L

E

E

R

G

G

K

D

L

L

D

G

V

F

P

I

T

S

A

A

A

A

M

-

A

S

K

K

Y

C

W

F

A

A

T

S

D

D

L

V

Q

A

C

M

K

E

V

V

L

T

D

T

E

D

C

A

V

V

Q
|
Q

L
|
L

H

F

G

G

G
|
G

Y

Y

G

G

F

Y

M

T

W

Q

E

D

Y

F

P

P

I

V

A

E

R

R

A

M

W

M

A

R

D

D

A

A

R

K

V
x
I

Q

T

R

Q

I

I

Y
|
Y

A
x
E

G

G

T
|
T

N

N

E
x
Q

I

I

M

Q

K

R

E

V

I

V

I

M

A

S

R
|
R

A

A

L

L

active site: L116 (≠ M126), S117 (≠ T127), T233 (≠ E243), E353 (≠ A364), R365 (= R376)
binding dihydroflavine-adenine dinucleotide: Y114 (≠ I124), L116 (≠ M126), S117 (≠ T127), G122 (= G132), S123 (= S133), W147 (≠ F157), I148 (= I158), T149 (= T159), R259 (= R269), F262 (= F272), V266 (≠ I276), N269 (≠ F279), Q326 (= Q337), L327 (= L338), G330 (= G341), I348 (≠ V359), Y352 (= Y363), T355 (= T366), Q357 (≠ E368)

1jqiA Crystal structure of rat short chain acyl-coa dehydrogenase complexed with acetoacetyl-coa (see paper)
38% identity, 97% coverage: 9:375/378 of query aligns to 8:376/384 of 1jqiA

query
sites
1jqiA

P

P

E

E

-

T

H

H

E

Q

L

M

F

L

R

R

E

Q

S

T

V

C

R

R

T

D

F

F

L

A

E

E

K

K

D

E

A

L

A

V

P

P

F

I

H

A

G

A

H

Q

W

L

E

D

K

K

Q

E

G

H

Y

L

I

F

D

P

R

T

R

S

L

Q

W

V

N

K

K

K

A

M

G

G

E

E

A

L

G

G

M

L

L

L

C

A

S

M

H

D

L

V

P

P

E

E

E

E

Y

L

G

S

G

G

L

A

G

G

A

L

D

D

F

Y

L

L

Y

A

S

Y

A

S

V

I

V

A

I

L

E

E

E

E

I

I

S

S

R

R

-

G

L

C

G

A

L

S

T

T

G

G

I

V

G

I

F

M

S

S

L

V

H

N

S

N

D

S

I
x
L

V

Y

A

L

P

G

Y

P

I

I

L

L

H

K

Y

F

G

G

S

S

E

S

A

Q

L

Q

K

K

H

Q

K

Q

Y

W

L

I

P

T

K

P

L

F

I

T

S

N

G

G

E

D

M

K

V

I

T

G

A

C

I
x
F

A

A

M
x
L

T
x
S

E

E

P

P

G

G

A

N

G
|
G

S
|
S

D

D

L

A

Q

G

G

A

V

A

K

S

T

T

T

T

A

A

V

R

L

E

D

E

G

G

D

D

E

S

Y

W

V

V

I

L

N

N

G

G

S

T

K

K

T

A

F
x
W

I

I

T
|
T

N

N

G

S

Y

W

L

E

A

A

E

S

L

A

V

T

I

V

V

V

V

F

A

A

K

S

T

T

D

D

P

R

K

S

A

R

G

Q

A

N

K

K

G

G

T

I

S

S

L

A

F

F

L

L

V

V

E

P

A

M

D

P

T

T

P

P

G

G

F

L

D

T

K

L

G

G

K

K

R

K

L

E

E

D

K

K

V

L

G

G

M

I

K

R

A

A

Q

S

D

S

T

T

S

A

E

N

L

L

F

I

F

F

Q

E

D

D

V

C

R

R

V

I

P

P

K

K

E

E

N

N

L

L

L

L

G

G

Q

E

A

P

G

G

M

M

G

G

F
|
F

A

K

Y

I

L

A

M
|
M

Q
|
Q

E

T

L
|
L

P
x
D

Q

M

E

G

R
|
R

L

I

T

G

V

I

A

A

I

S

G

Q

A

A

L

L

S

G

S

I

A

A

E

Q

A

A

A

S

L

L

E

D

W

C

T

A

L

V

E

K

Y

Y

T

A

R

E

E

N

R
|
R

K

H

A

A

F
|
F

G

G

K

A

A

P

I

L

A

T

D

K

F
x
L

Q

Q

N

N

T

I

R

Q

F

F

K

K

L

L

A

A

E

D

M

M

A

A

T

L

E

A

I

L

Q

E

I

S

G

A

R

R

V

L

F

L

V

T

D

W

K

R

C

A

M

A

A

M

L

L

H

K

L

D

E

N

G

K

K

K

L

P

D

F

V

T

P

K

T

E

A

S

A

A

M

M

A

A

K

K

Y

L

W

A

A

A

T

S

D

E

L

A

Q

A

C

T

K

A

V

I

L

S

D

H

E

Q

C

A

V

I

Q
|
Q

L
x
I

H

L

G

G

G
|
G

Y

M

G

G

F

Y

M

V

W

T

E

E

Y

M

P

P

I

A

A

E

R

R

A

Y

W

Y

A

R

D

D

A

A

R

R

V
x
I

Q

T

R

E

I

I

Y
|
Y

A
x
E

G
|
G

T
|
T

N

S

E
|
E

I
|
I

M

Q

K

R

E

L

I

V

I

I

A

A

binding acetoacetyl-coenzyme a: L95 (≠ I94), F125 (≠ I124), S134 (= S133), F234 (= F233), M238 (= M237), Q239 (= Q238), L241 (= L240), D242 (≠ P241), R245 (= R244), Y364 (= Y363), E365 (≠ A364), G366 (= G365)
binding flavin-adenine dinucleotide: F125 (≠ I124), L127 (≠ M126), S128 (≠ T127), G133 (= G132), S134 (= S133), W158 (≠ F157), T160 (= T159), R270 (= R269), F273 (= F272), L280 (≠ F279), Q338 (= Q337), I339 (≠ L338), G342 (= G341), I360 (≠ V359), T367 (= T366), E369 (= E368), I370 (= I369)

Sites not aligning to the query:

active site: 377

P15651 Short-chain specific acyl-CoA dehydrogenase, mitochondrial; SCAD; Butyryl-CoA dehydrogenase; EC 1.3.8.1 from Rattus norvegicus (Rat) (see 2 papers)
38% identity, 97% coverage: 9:375/378 of query aligns to 35:403/412 of P15651

query
sites
P15651

P

P

E

E

-

T

H

H

E

Q

L

M

F

L

R

R

E

Q

S

T

V

C

R

R

T

D

F

F

L

A

E

E

K

K

D

E

A

L

A

V

P

P

F

I

H

A

G

A

H

Q

W

L

E

D

K

K

Q

E

G

H

Y

L

I

F

D

P

R

T

R

S

L

Q

W

V

N

K

K

K

A

M

G

G

E

E

A

L

G

G

M

L

L

L

C

A

S

M

H

D

L

V

P

P

E

E

E

E

Y

L

G

S

G

G

L

A

G

G

A

L

D

D

F

Y

L

L

Y

A

S

Y

A

S

V

I

V

A

I

L

E

E

E

E

I

I

S

S

R

R

-

G

L

C

G

A

L

S

T

T

G

G

I

V

G

I

F

M

S

S

L

V

H

N

S

N

D

S

I

L

V

Y

A

L

P

G

Y

P

I

I

L

L

H

K

Y

F

G

G

S

S

E

S

A

Q

L

Q

K

K

H

Q

K

Q

Y

W

L

I

P

T

K

P

L

F

I

T

S

N

G

G

E

D

M

K

V

I

T

G

A

C

I
x
F

A
|
A

M
x
L

T
x
S

E
|
E

P
|
P

G
|
G

A
x
N

G
|
G

S
|
S

D

D

L

A

Q

G

G

A

V

A

K

S

T

T

T

T

A

A

V

R

L

E

D

E

G

G

D

D

E

S

Y

W

V

V

I

L

N

N

G

G

S

T

K

K

T

A

F
x
W

I
|
I

T
|
T

N

N

G

S

Y

W

L

E

A

A

E

S

L

A

V

T

I

V

V

V

V

F

A

A

K

S

T

T

D

D

P

R

K

S

A

R

G

Q

A

N

K

K

G

G

T

I

S

S

L

A

F

F

L

L

V

V

E

P

A

M

D

P

T

T

P

P

G

G

F

L

D

T

K

L

G

G

K

K

R

K

L

E

E

D

K

K

V

L

G

G

M

I

K

R

A

A

Q

S

D

S

T

T

S

A

E

N

L

L

F

I

F

F

Q

E

D

D

V

C

R

R

V

I

P

P

K

K

E

E

N

N

L

L

L

L

G

G

Q

E

A

P

G

G

M

M

G

G

F

F

A

K

Y

I

L

A

M

M

Q

Q

E

T

L

L

P
x
D

Q
x
M

E
x
G

R
|
R

L

I

T

G

V

I

A

A

I

S

G

Q

A

A

L

L

S

G

S

I

A

A

E

Q

A

A

A

S

L

L

E

D

W

C

T

A

L

V

E

K

Y

Y

T

A

R

E

E

N

R
|
R

K

H

A

A

F

F

G

G

K

A

A

P

I

L

A

T

D

K

F

L

Q

Q

N

N

T

I

R

Q

F

F

K

K

L

L

A

A

E

D

M

M

A

A

T

L

E

A

I

L

Q

E

I

S

G

A

R

R

V

L

F

L

V

T

D

W

K

R

C

A

M

A

A

M

L

L

H

K

L

D

E

N

G

K

K

K

L

P

D

F

V

T

P

K

T

E

A

S

A

A

M

M

A

A

K

K

Y

L

W

A

A

A

T

S

D

E

L

A

Q

A

C

T

K

A

V

I

L

S

D

H

E

Q

C

A

V

I

Q
|
Q

L
x
I

H
x
L

G
|
G

G
|
G

Y

M

G

G

F

Y

M

V

W

T

E

E

Y

M

P

P

I

A

A

E

R

R

A

Y

W

Y

A

R

D

D

A

A

R

R

V

I

Q

T

R

E

I

I

Y

Y

A
x
E

G

G

T
|
T

N
x
S

E
|
E

I

I

M

Q

K

R

E

L

I

V

I

I

A

A

152:161 (vs. 124:133, 60% identical) binding
S161 (= S133) binding
WIT 185:187 (≠ FIT 157:159) binding
DMGR 269:272 (≠ PQER 241:244) binding
R297 (= R269) binding
QILGG 365:369 (≠ QLHGG 337:341) binding
E392 (≠ A364) active site, Proton acceptor
TSE 394:396 (≠ TNE 366:368) binding

Sites not aligning to the query:

1:24 modified: transit peptide, Mitochondrion

7w0jE Acyl-coa dehydrogenase, tfu_1647
37% identity, 99% coverage: 6:378/378 of query aligns to 4:380/382 of 7w0jE

query
sites
7w0jE

L

L

F

Y

S

R

P

P

-

T

-

E

E

E

H

H

E

E

L

A

F

L

R

R

E

E

S

A

V

I

R

R

T

S

F

V

L

A

E

E

K

D

D

K

A

I

A

A

P

P

F

H

H

A

G

A

H

D

W

V

E

D

K

E

Q

Q

G

S

Y

R

I

F

D

P

R

Q

R

E

L

A

W

Y

N

E

K

A

A

L

G

R

E

A

A

S

G

D

M

F

L

H

C

A

S

P

H

H

L

V

P

A

E

E

E

E

Y

Y

G

G

G

G

L

V

G

G

A

A

D

D

F

A

L

L

Y

A

S

T

A

C

V

I

V

V

I

I

E

E

E

E

I

I

S

A

R

R

L

V

G

C

L

A

T

S

G

S

I

S

G

L

F

I

S

P

L

A

H

V

S

N

D

K

I

L

V

G

A

S

P

M

Y

P

I

L

L

I

H

L

Y

S

G

G

S

S

E

D

A

E

L

V

K

K

H

Q

K

R

Y

Y

L

L

P

P

K

E

L

L

I

A

S

S

G

G

E

E

M

A

V

M

T

F

A

S

I

Y

A

G

M

L

T
x
S

E

E

P

R

G

E

A

A

G

G

S

S

D

D

L

T

Q

A

G

S

V

M

K

R

T

T

T

R

A

A

V

V

L

R

D

D

G

G

D

D

E

D

Y

W

V

I

I

L

N

N

G

G

S

Q

K

K

T

S

F
x
W

I

I

T

T

N

N

G

A

Y

G

L

I

A

S

E

K

L

Y

V

Y

I

T

V

V

V

M

A

A

K

V

T

T

D

D

P

P

K

D

A

-

G

G

A

P

K

R

G

G

T

R

S

N

L

I

-

S

-

A

F

F

L

V

V

V

E

H

A

I

D

D

T

D

P

P

G

G

F

F

D

S

K

F

G

G

K

E

R

P

L

E

E

R

K

K

V

L

G

G

M

I

K

K

A

G

Q

S

D

P

T

T

S

R

E

E

L

L

F

I

F

F

Q

D

D

N

V

V

R

R

V

I

P

P

K

G

E

D

N

R

L

L

L

V

G

G

Q

K

A

V

G

G

M

E

G

G

F

L

A

R

Y

T

L

A

M

L

Q

R

E

T

L

L

P

D

Q

H

E

T

R

R

L

V

T

T

V

I

A

G

I

A

G

Q

A

A

L

V

S

G

S

I

A

A

E

Q

A

G

A

A

L

L

E

D

W

Y

T

A

L

L

E

G

Y

Y

T

V

R

K

E

E

R
|
R

K

K

A
x
Q

F
|
F

G

G

K

K

A

A

I
|
I

A

A

D

D

F
|
F

Q

Q

N

G

T
x
I

R

Q

F

F

K

M

L

L

A

A

E

D

M

M

A

A

T

M

E

K

I

L

Q

E

I

A

G

A

R

R

V

Q

F

M

V

V

D

Y

K

V

C

A

M

A

A

A

L

K

H

S

L

E

E

R

G

D

K

D

L

A

D

D

V

L

P

S

-

F

T

Y

A

G

A

A

M

A

A

A

K

K

Y

C

W

F

A

A

T

S

D

D

L

V

Q

A

C

M

K

E

V

I

L

T

D

T

E

D

C

A

V

V

Q
|
Q

L
|
L

H

L

G

G

G
|
G

Y

Y

G

G

F

Y

M

T

W

R

E

D

Y

Y

P

P

I

V

A

E

R

R

A

M

W

M

A

R

D

D

A

A

R

K

V

I

Q

T

R

Q

I

I

Y
|
Y

A
x
E

G

G

T
|
T

N

N

E
x
Q

I
|
I

M

Q

K

R

E

V

I

V

I

M

A

A

R

R

A

Q

L

L

binding [[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2R,3S,4S)-5-azanyl-2,3,4-tris(oxidanyl)pentyl] hydrogen phosphate: S127 (≠ T127), W157 (≠ F157), R270 (= R269), Q272 (≠ A271), F273 (= F272), I277 (= I276), F280 (= F279), I283 (≠ T282), Q339 (= Q337), L340 (= L338), G343 (= G341), Y365 (= Y363), E366 (≠ A364), T368 (= T366), Q370 (≠ E368), I371 (= I369)

4n5fA Crystal structure of a putative acyl-coa dehydrogenase with bound fadh2 from burkholderia cenocepacia j2315
35% identity, 99% coverage: 6:378/378 of query aligns to 5:378/378 of 4n5fA

query
sites
4n5fA

L

L

F

Y

S

T

P

E

E

D

H

Q

E

R

L

M

F

I

R

R

E

D

S

A

V

A

R

R

T

A

F

F

L

A

E

T

K

E

D

M

A

L

A

A

P

P

F

N

H

A

G

A

H

Q

W

W

E

D

K

H

Q

D

G

A

Y

H

I

L

D

P

R

D

R

A

L

I

W

V

N

A

K

Q

A

L

G

G

E

E

A

L

G

G

M

L

L

L

C

G

S

M

H

I

L

V

P

P

E

Q

E

E

Y

L

G

G

G

G

L

S

G

Y

A

T

D

D

F

Y

L

V

Y

A

S

Y

A

A

V

L

V

A

I

M

E

E

E

E

I

V

S

A

R

A

-

G

L

D

G

A

L

A

T

C

G

A

I

T

G

M

F

M

S

S

L

V

H

H

S

N

D

S

I

V

V

G

A

C

P

G

Y

P

I

I

L

L

H

G

Y

F

G

G

S

T

E

P

A

A

L

Q

K

K

H

D

K

R

Y

W

L

L

P

A

K

D

L

M

I

A

S

A

G

G

E

R

M

V

V

I

T

G

A

A

I

F

A

C

M
x
L

T
|
T

E

E

P

P

G

H

A

A

G
|
G

S
|
S

D

E

L

A

Q

N

G

N

V

L

K

R

T

T

T

R

A

A

V

E

L