SitesBLAST

P07464 Galactoside O-acetyltransferase; GAT; Acetyl-CoA:galactoside 6-O-acetyltransferase; Thiogalactoside acetyltransferase; Thiogalactoside transacetylase; EC 2.3.1.18 from Escherichia coli (strain K12) (see 3 papers)
38% identity, 99% coverage: 1:181/182 of query aligns to 1:181/203 of P07464

query
sites
P07464

M
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M

A

N

M

M

S

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E

M

K

T

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R

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I

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R

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A

G

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A

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K

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H

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D
x
S

Y

Y

G

G

Y

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N

N

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H

H

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I

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E

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G

N

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N

F

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N

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x
D

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H

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I

I

G

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A

D

G

N

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H

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G

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M

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P

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I

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W

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x
S

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T

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I

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G

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D

D

N

A

S

V

V

I

I

G

G

A
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A

G

G

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S

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T
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T

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x
K

D

D

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P

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M1 (= M1) modified: Initiator methionine, Removed; Partial
D17 (≠ P17) binding
S71 (≠ D71) binding
N85 (= N85) binding in other chain; binding in other chain
D93 (≠ V93) binding
H115 (= H115) mutation to A: Results in an 1800-fold decrease in catalytic activity.
S142 (≠ G142) binding in other chain
A160 (= A160) binding in other chain
TK 165:166 (≠ TR 165:166) binding
R180 (= R180) binding

Sites not aligning to the query:

183 binding in other chain

1krvA Galactoside acetyltransferase in complex with coa and pnp-beta-gal (see paper)
39% identity, 99% coverage: 1:181/182 of query aligns to 2:180/201 of 1krvA

query
sites
1krvA

M

M

A

P

M

M

S

T

E

E

K

R

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K

R

M

-

L

-

A

A

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G

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x
D

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M

D

C

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K

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x
R

A

L

A

R

A

G

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K

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M

M

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Y

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E

Y

F

N

N

A

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M

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F

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Y

Y

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G

Y

S

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N

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L

I

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G

N

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F
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Y

L

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N
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N

F

F

N

N

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L

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I

L
x
V

D

D

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x
D

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Y

E

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G

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G

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S

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x
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H

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E

A

L

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E
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M

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Y

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S

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F

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P

I

I

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T

I

I

G

G

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N

N

V

V

W
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W

V

I

G
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G

G
x
S

G

H

A

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I

I

L
x
N

P

P

G

G

V

V

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T

I

I

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G

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D

D

N

A

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A
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A

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G

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binding 4-nitrophenyl beta-D-galactopyranoside: D16 (≠ P17), R25 (≠ Q26), S70 (≠ D71), Y82 (≠ F83), N84 (= N85), V90 (≠ L91), D92 (≠ V93), M126 (≠ E127)
binding coenzyme a: A104 (≠ G105), S110 (≠ Y111), T112 (≠ A113), W138 (= W139), G140 (= G141), S141 (≠ G142), N146 (≠ L147), A159 (= A160), P177 (= P178), R179 (= R180)

Sites not aligning to the query:

binding coenzyme a: 182

1kruA Galactoside acetyltransferase in complex with iptg and coenzyme a (see paper)
39% identity, 99% coverage: 1:181/182 of query aligns to 2:180/201 of 1kruA

query
sites
1kruA

M

M

A

P

M

M

S

T

E

E

K

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I

K

R

M

-

L

-

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A

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G

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L

Y

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M

D

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A

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K

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W

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M

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R

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Y

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N

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M

L

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A

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G

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A

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I

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Y

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G

Y

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N

N

I

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H

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L

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G

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G

N

A

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F
x
Y

L

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N

F

F

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N

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I

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x
V

D

D

V
x
D

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Y

E

T

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I

I

G

G

A

D

G

N

A

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L

I

I

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A

N

V

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L

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H

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P

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E

E

A

L

R

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R

K

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M

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P

I

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N

T

I

I

G

G

R

N

N

N

V

V

W
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W

V

I

G
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G

G
x
S

G

H

A

V

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V

I

I

L

N

P

P

G

G

V

V

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T

I

I

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G

D

D

D

N

A

S

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V

I

I

G
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G

A
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A

G

G

S

S

V

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V

T

T

R

K

D

D

V

I

P

P

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P

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N

A

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A

G

G

N

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P

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V

binding coenzyme a: H114 (= H115), W138 (= W139), G140 (= G141), S141 (≠ G142), G158 (= G159), A159 (= A160), P177 (= P178)
binding 1-methylethyl 1-thio-beta-D-galactopyranoside: D16 (≠ P17), P22 (≠ L23), R25 (≠ Q26), W62 (≠ V63), S70 (≠ D71), Y82 (≠ F83), Y82 (≠ F83), N84 (= N85), V90 (≠ L91), D92 (≠ V93), L102 (≠ Q103), H114 (= H115), M126 (≠ E127)

Sites not aligning to the query:

binding coenzyme a: 182

1krrA Galactoside acetyltransferase in complex with acetyl-coenzyme a (see paper)
39% identity, 99% coverage: 1:181/182 of query aligns to 2:180/200 of 1krrA

query
sites
1krrA

M

M

A

P

M

M

S

T

E

E

K

R

Q

I

K

R

M

-

L

-

A

A

G

G

E

K

L

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Y

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Y

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D

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M

D

C

P

E

E

G

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K

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L

A

R

A

G

K

K

A

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W

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M

M

V

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R

E

Y

F

N

N

A

H

A

S

L

H

A

P

A

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S

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A

K

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G

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A

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P

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C

F

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S

Y

Y

G

G

Y

S

N

N

I

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H

H

L

I

G

G

E

R

G

N

A

F

F

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L

A

N
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N

F

F

N

N

C

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I

L

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D

D

V

D

V

Y

E

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I

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G

A

D

G

N

A

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I

G
x
A

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P

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V

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H

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D

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P

H

E

E

A

L

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G

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M

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P

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T

I

I

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N

N

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W

W

V

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G
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G

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x
S

G

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A

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I

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I

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L
x
N

P

P

G

G

V

V

T

T

I

I

G

G

D

D

D

N

A

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V

V

I

I

G
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G

A
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A

G

G

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S

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I

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V

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T

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K

D

D

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P

A

P

G

N

A

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V
x
A

G

G

N

V

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P

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C

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R

I

V

binding acetyl coenzyme *a: N84 (= N85), I103 (= I104), A104 (≠ G105), P105 (= P106), T112 (≠ A113), H114 (= H115), G140 (= G141), S141 (≠ G142), N146 (≠ L147), G158 (= G159), A159 (= A160), A174 (≠ V175), P177 (= P178)

Sites not aligning to the query:

binding acetyl coenzyme *a: 182

3nz2J Crystal structure of hexapeptide-repeat containing-acetyltransferase vca0836 complexed with acetyl co enzyme a from vibrio cholerae o1 biovar eltor
41% identity, 99% coverage: 1:181/182 of query aligns to 2:180/185 of 3nz2J

query
sites
3nz2J

M

M

A

K

M

M

S

S

E

E

K

L

Q

E

K

K

M

M

L

L

A

K

G

G

E

E

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H

Y

F

Y

D

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G

G

A

D

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P

A

E

E

I

I

L

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A

A

D

L

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R

A

S

A

Q

A

A

K

G

A

R

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L

M

K

V

L

R

E

Y

I

N

N

A

Q

A

S

L

L

A

D

A

E

S

A

P

-

D

-

E

E

R

R

R

Y

A

A

L

L

L

Q

A

R

E

E

R

L

L

F

A

G

S

H

V

L

G

G

A

H

G

K

A

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C

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P

P

F

F

H

H

C

C

D

E

Y

F

G

G

Y

K

N

T

I

I

H

R

L

I

G

G

E

D

G

H

A

T

F

F

L

I

N

N

F

M

N

N

C

V

V

V

I

M

L

L

D

D

V

G

V

A

E

P

V

I

H

T

I

I

G

G

A

D

G

H

A

V

Q

L

I

I

G
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G

P

P

A

S

V

T

Q

Q

L

F

Y
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Y

T

T

A

A

D

S

H
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H

P

S

R

L

D

D

P

Y

E

R

A

R

R

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R

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S

A

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E

T

F

I

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C

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K

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P

I

I

N

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I

I

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D

N

D

V

V

W
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W

V

I

G
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G

G

G

G

N

A

V

I

V

I

I

L

N

P

Q

G

G

V

V

T

T

I

I

G

G

D

A

D

R

A

S

V

V

I

V

G
x
A

A
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A

G

N

S

S

V

V

T
x
N

R

Q

D

D

V

V

P

P

A

P

G

D

A

T

T

L

V

V

V
x
G

G

G

N
x
T

P
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P

A

A

R

R

I

I

binding acetyl coenzyme *a: G104 (= G105), Y110 (= Y111), H114 (= H115), W138 (= W139), G140 (= G141), A158 (≠ G159), A159 (= A160), N164 (≠ T165), G174 (≠ V175), T176 (≠ N177), P177 (= P178)

Sites not aligning to the query:

binding acetyl coenzyme *a: 182

3nz2C Crystal structure of hexapeptide-repeat containing-acetyltransferase vca0836 complexed with acetyl co enzyme a from vibrio cholerae o1 biovar eltor
41% identity, 98% coverage: 3:181/182 of query aligns to 1:177/183 of 3nz2C

query
sites
3nz2C

M

M

S

S

E

E

K

L

Q

E

K

K

M

M

L

L

A

K

G

G

E

E

L

H

Y

F

Y

D

P

G

G

A

D

S

P

A

E

E

I

I

L

E

A

A

D

L

Q

R

A

S

A

Q

A

A

K

G

A

R

W

L

M

K

V

L

R

E

Y

I

N

N

A

Q

A

S

L

L

A

D

A

E

S

A

P

-

D

-

E

E

R

R

R

Y

A

A

L

L

L

Q

A

R

E

E

R

L

L

F

A

G

S

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L

G

G

A

H

G

K

A

S

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C

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V

R

Q

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P

F

F

H

H

C

C

D

E

Y

F

G

G

Y

K

N

T

I

I

H

R

L

I

G

G

E

D

G

H

A

T

F

F

L

I

N

N

F

M

N

N

C

V

V

V

I

M

L

L

D

D

V

G

V

A

E

P

V

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H

T

I

I

G

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A

D

G

H

A

V

Q

L

I

I

G

G

P

P

A

S

V

T

Q

Q

L

F

Y
|
Y

T

T

A

A

D

S

H
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H

P

S

R

L

D

D

P

Y

E

R

A

R

R

R

R

Q

S

A

G

W

V

E

E

T

F

I

G

C

R

K

P

P

I

I

N

V

I

I

G

E

R

D

N

D

V

V

W

W

V

I

G
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G

G

G

G

N

A

V

I

V

I

I

L

N

P

Q

G

G

V

V

T

T

I

I

G

G

D

A

D

R

A

S

V

V

I

V

G
x
A

A
|
A

G
x
N

S

S

V

V

T
x
N

R

Q

D

D

V

V

P

P

A

P

G

D

A

T

T

L

V

V

V
x
G

G

G

N
x
T

P
|
P

A

A

R

R

I

I

binding acetyl coenzyme *a: Y107 (= Y111), H111 (= H115), G137 (= G141), A155 (≠ G159), A156 (= A160), N161 (≠ T165), G171 (≠ V175), T173 (≠ N177), P174 (= P178)
binding magnesium ion: N157 (≠ G161), T173 (≠ N177)

Sites not aligning to the query:

binding acetyl coenzyme *a: 178, 179

3ectA Crystal structure of the hexapeptide-repeat containing- acetyltransferase vca0836 from vibrio cholerae
39% identity, 96% coverage: 7:181/182 of query aligns to 2:170/176 of 3ectA

query
sites
3ectA

Q

E

K

K

M

M

L

L

A

K

G

G

E

H

L

-

Y

-

P

-

G

A

D

S

P

A

E

E

I

I

L

E

A

A

D

L

Q

R

A

S

A

Q

A

A

K

G

A

R

W

L

M

K

V

L

R

E

Y

I

N

N

A

Q

A

S

L

L

A

D

A

E

S

A

P

-

D

-

E

E

R

R

R

Y

A

A

L

L

L

Q

A

R

E

E

R

L

L

F

A

G

S

H

V

L

G

G

A

H

G

K

A

S

V

C

I

V

R

Q

P

P

F

F

H

H

C

C

D

E

Y

F

G

G

Y

K

N

T

I

I

H

R

L

I

G

G

E

D

G

H

A

T

F

F

L

I

N

N

F

M

N

N

C

V

V

V

I

M

L

L

D

D

V

G

V

A

E

P

V

I

H

T

I

I

G

G

A

D

G

H

A

V

Q

L

I

I

G

G

P

P

A

S

V

T

Q

Q

L

F

Y

Y

T

T

A

A

D

S

H

H

P

S

R

L

D

D

P

Y

E

R

A

R

R

R

R

Q

S

A

G

W

V

E

E

T

F

I

G

C

R

K

P

P

I

I

N

V

I

I

G

E

R

D

N

D

V

V

W

W

V

I

G

G

G

N

A

V

I

V

I

I

L

N

P

Q

G
|
G

V

V

T

T

I

I

G

G

D

A

D

R

A

S

V

V

I

V

G

A

A

A

G

N

S

S

V

V

T

N

R

Q

D
|
D

V

V

P

P

A

P

G

D

A

T

T

L

V

V

V

G

G

G

N

T

P

P

A

A

R

R

I

I

binding calcium ion: G138 (= G149), D156 (= D167)

A1ADJ6 Polysialic acid O-acetyltransferase; Capsule O-acetyl transferase; EC 2.3.1.136 from Escherichia coli O1:K1 / APEC (see paper)
38% identity, 51% coverage: 89:181/182 of query aligns to 183:276/307 of A1ADJ6

query
sites
A1ADJ6

V

V

I

V

L

T

D

D

V

K

V

C

E

N

V

V

H

T

I

I

G

G

A

H

G

D

A

C

Q

M

I

I

G

A

P

R

A

D

V

V

Q

I

L

L

Y

R

T

A

A

S

D

D

-

G

H
|
H

P

P

R

I

D

F

P

D

E

I

A

H

R

S

R

K

S

K

G

R

V

I

E

N

F

W

G

A

R

K

P

D

I

I

N

I

I

I

G

S

R

S

N

Y

V

V

W
|
W

V

V

G

G

G

R

G

N

A

V

I

S

I

I

L

M

P

K

G

G

V

V

T

S

I

V

G

G

D

S

D

G

A

S

V

V

I

I

G

G

A

Y

G

G

S

S

V

I

V

V

T

T

R

K

D

D

V

V

P

P

A

S

G

M

A

C

T

A

V

A

G

G

N

N

P

P

A

A

R

K

I

I

H210 (= H115) mutation to A: Loss of activity.
W234 (= W139) mutation to A: Loss of activity.

4mzuB Crystal structure of fdtd, a bifunctional ketoisomerase/n- acetyltransferase from shewanella denitrificans (see paper)
38% identity, 47% coverage: 96:181/182 of query aligns to 65:139/290 of 4mzuB

query
sites
4mzuB

V

I

H

H

I

I

G

Q

A

D

G

D

A

V

Q
x
F

I

I

G

G

P

P

A
x
N

V

V

Q

-

L

-

Y

-

T

T

A

F

D
x
T

H
x
N

P
x
D

R
x
K

D

Q

P
|
P

E

R

A

S

R

L

R

K

S

T

G

I

V

V

E

K

F

K

G

G

R

A

P

S

I

I

N

-

I

-

G

-

R

-

N

-

V

-

W

-

V

-

G
|
G

G
x
A

G

N

A

S

I

T

I

I

L

L

P

P

G

G

V

I

T

L

I

I

G

G

D

E

D

N

A

A

V

M

I

V

G
|
G

A
|
A

G

G

S

A

V

V

V

I

T
|
T

R
x
K

D

N

V

V

P

P

A

D

G

N

A

A

T

I

V

V

V
x
I

G

G

N

N

P

P

A

G

R
|
R

I

I

binding coenzyme a: F72 (≠ Q103), T80 (≠ D114), N81 (≠ H115), D82 (≠ P116), K83 (≠ R117), P85 (= P119), G99 (= G141), A100 (≠ G142), G117 (= G159), A118 (= A160), T123 (= T165), K124 (≠ R166), I133 (≠ V175), R138 (= R180)
binding magnesium ion: N76 (≠ A107)

Sites not aligning to the query:

binding coenzyme a: 199
binding magnesium ion: 58
binding thymine: 20, 21, 38, 39
binding thymidine-5'-diphosphate: 171, 176, 189, 191, 202, 203, 270

4mzuF Crystal structure of fdtd, a bifunctional ketoisomerase/n- acetyltransferase from shewanella denitrificans (see paper)
38% identity, 47% coverage: 96:181/182 of query aligns to 65:140/294 of 4mzuF

query
sites
4mzuF

V

I

H

H

I

I

G

Q

A

D

G

D

A

V

Q

F

I

I

G

G

P

P

A

N

V

V

Q

T

L

F

Y

T

T
x
N

A
x
D

D
x
K

H

Q

P

P

R

R

D

-

P

-

E

S

A

K

R

I

R

K

S

T

G

I

V

V

E

K

F

K

G

G

R

A

P

S

I

I

N

-

I

-

G

-

R

-

N

-

V

-

W

-

V

-

G
|
G

G
x
A

G

N

A

S

I

T

I

I

L
|
L

P

P

G

G

V

I

T

L

I

I

G

G

D

E

D

N

A

A

V

M

I

V

G
|
G

A
|
A

G

G

S

A

V

V

V

I

T
|
T

R
x
K

D

N

V

V

P

P

A

D

G

N

A

A

T

I

V

V

V
x
I

G

G

N
|
N

P

P

A

G

R
|
R

I

I

binding coenzyme a: N81 (≠ T112), D82 (≠ A113), K83 (≠ D114), G100 (= G141), A101 (≠ G142), L106 (= L147), G118 (= G159), A119 (= A160), T124 (= T165), K125 (≠ R166), I134 (≠ V175), N136 (= N177), R139 (= R180)

Sites not aligning to the query:

binding thymine: 20, 21, 38
binding thymidine-5'-diphosphate: 189, 203, 205, 270

7s42A Crystal structure of an n-acetyltransferase from helicobacter pullorum in the presence of coenzyme a and dtdp-3-acetamido-3,6-dideoxy-d- galactose (see paper)
34% identity, 55% coverage: 69:168/182 of query aligns to 42:135/145 of 7s42A

query
sites
7s42A

H

H

C

C

D
x
F

Y

I

G
x
E

Y

N

N

D

I

V

H

K

L

I

G

G

E

N

G

N

A

V

F

T

L

I

N

K

F

C

N

G

C

V

V

Q

I

I

L
x
W

D

D

V

G

V

I

E

E

V

I

H

E

I

-

G

-

A

D

G

D

A

V

Q

F

I

I

G

G

P

P

A

N

V

V

Q

T

L

F

Y
x
C

T
x
N

A
x
D

D
x
K

H
x
Y

P
|
P

R

R

D

S

P

K

E

Q

A
x
Y

R

P

R

K

S

-

G

-

V

-

E

E

F

F

G

S

R

K

P

T

I

I

N

-

I

I

G

K

R

K

N

G

V

A

W

S

V

I

G

G

G

A

G

N

A

A

I

T

I

I

L
|
L

P

P

G

G

V

I

T

T

I

I

G

G

D

E

D

N

A

A

V

M

I

I

G

G

A

A

G

G

S

A

V

I

V

V

T
|
T

R
x
K

D

D

V

V

binding [(2~{R},3~{R},4~{S},5~{R},6~{R})-4-acetamido-6-methyl-3,5-bis(oxidanyl)oxan-2-yl] [[(2~{R},3~{S},5~{R})-5-[5-methyl-2,4-bis(oxidanylidene)pyrimidin-1-yl]-3-oxidanyl-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] hydrogen phosphate: F44 (≠ D71), E46 (≠ G73), W64 (≠ L91), N83 (≠ T112), D84 (≠ A113), Y92 (≠ A121)
binding coenzyme a: C82 (≠ Y111), D84 (≠ A113), K85 (≠ D114), Y86 (≠ H115), P87 (= P116), L114 (= L147), T132 (= T165), K133 (≠ R166)

7s41A Crystal structure of an n-acetyltransferase from helicobacter pullorum in the presence of coenzyme a and dtdp-3-acetamido-3,6-dideoxy-d- glucose (see paper)
34% identity, 55% coverage: 69:168/182 of query aligns to 42:135/145 of 7s41A

query
sites
7s41A

H

H

C

C

D
x
F

Y

I

G
x
E

Y

N

N

D

I

V

H

K

L

I

G

G

E

N

G

N

A

V

F

T

L

I

N

K

F

C

N

G

C

V

V

Q

I

I

L
x
W

D

D

V

G

V

I

E

E

V

I

H

E

I

-

G

-

A

D

G

D

A

V

Q

F

I

I

G

G

P

P

A

N

V

V

Q

T

L

F

Y
x
C

T
x
N

A
x
D

D
x
K

H
x
Y

P
|
P

R

R

D

S

P

K

E

Q

A
x
Y

R

P

R

K

S

-

G

-

V

-

E

E

F

F

G

S

R

K

P

T

I

I

N

-

I

I

G

K

R

K

N

G

V

A

W

S

V

I

G

G

G

A

G

N

A

A

I

T

I

I

L
|
L

P

P

G

G

V

I

T

T

I

I

G

G

D

E

D

N

A

A

V

M

I

I

G

G

A

A

G

G

S

A

V

I

V

V

T
|
T

R
x
K

D

D

V

V

binding [(2~{R},3~{R},4~{S},5~{S},6~{R})-4-acetamido-6-methyl-3,5-bis(oxidanyl)oxan-2-yl] [[(2~{R},3~{S},5~{R})-5-[5-methyl-2,4-bis(oxidanylidene)pyrimidin-1-yl]-3-oxidanyl-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] hydrogen phosphate : F44 (≠ D71), E46 (≠ G73), W64 (≠ L91), N83 (≠ T112), D84 (≠ A113), Y92 (≠ A121)
binding coenzyme a: C82 (≠ Y111), D84 (≠ A113), K85 (≠ D114), Y86 (≠ H115), P87 (= P116), L114 (= L147), T132 (= T165), K133 (≠ R166)

7s3wA Crystal structure of an n-acetyltransferase from helicobacter pullorum in the presence of coenzyme a and dtdp-3-amino-3,6-dideoxy-d- galactose (see paper)
34% identity, 55% coverage: 69:168/182 of query aligns to 42:135/145 of 7s3wA

query
sites
7s3wA

H

H

C

C

D
x
F

Y

I

G
x
E

Y

N

N

D

I

V

H

K

L

I

G

G

E

N

G

N

A

V

F

T

L

I

N

K

F

C

N

G

C

V

V

Q

I

I

L

W

D

D

V

G

V

I

E

E

V

I

H

E

I

-

G

-

A

D

G

D

A

V

Q

F

I

I

G

G

P

P

A

N

V

V

Q

T

L

F

Y
x
C

T

N

A
x
D

D
x
K

H
x
Y

P
|
P

R

R

D

S

P

K

E

Q

A
x
Y

R

P

R

K

S

-

G

-

V

-

E

E

F

F

G

S

R

K

P

T

I

I

N

-

I

I

G

K

R

K

N

G

V

A

W

S

V

I

G

G

G

A

G

N

A

A

I

T

I

I

L
|
L

P

P

G

G

V

I

T

T

I

I

G

G

D

E

D

N

A

A

V

M

I

I

G

G

A

A

G

G

S

A

V

I

V

V

T
|
T

R
x
K

D

D

V

V

binding oxidized coenzyme a: C82 (≠ Y111), D84 (≠ A113), K85 (≠ D114), Y86 (≠ H115), P87 (= P116), L114 (= L147), T132 (= T165), K133 (≠ R166)
binding (3R,4S,5R,6R)-4-amino-3,5-dihydroxy-6-methyloxan-2-yl][hydroxy-[[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy]phosphoryl] hydrogen phosphate: F44 (≠ D71), E46 (≠ G73), D84 (≠ A113), Y92 (≠ A121)

7s3uA Crystal structure of an n-acetyltransferase from helicobacter pullorum in the presence of coenzyme a and dtdp-3-amino-3,6-dideoxy-d-glucose (see paper)
34% identity, 55% coverage: 69:168/182 of query aligns to 42:135/145 of 7s3uA

query
sites
7s3uA

H

H

C

C

D
x
F

Y

I

G
x
E

Y

N

N

D

I

V

H

K

L

I

G

G

E

N

G

N

A

V

F

T

L

I

N

K

F

C

N

G

C

V

V

Q

I

I

L

W

D

D

V

G

V

I

E

E

V

I

H

E

I

-

G

-

A

D

G

D

A

V

Q

F

I

I

G

G

P

P

A

N

V

V

Q

T

L

F

Y
x
C

T
x
N

A
x
D

D
x
K

H
x
Y

P
|
P

R

R

D

S

P

K

E

Q

A
x
Y

R

P

R

K

S

-

G

-

V

-

E

E

F

F

G

S

R

K

P

T

I

I

N

-

I

I

G

K

R

K

N

G

V

A

W

S

V

I

G

G

G

A

G

N

A

A

I

T

I

I

L
|
L

P

P

G

G

V

I

T

T

I

I

G

G

D

E

D

N

A

A

V

M

I

I

G

G

A

A

G

G

S

A

V

I

V

V

T
|
T

R
x
K

D

D

V

V

binding coenzyme a: C82 (≠ Y111), N83 (≠ T112), D84 (≠ A113), K85 (≠ D114), Y86 (≠ H115), P87 (= P116), L114 (= L147), T132 (= T165), K133 (≠ R166)
binding [(3R,4S,5S,6R)-4-amino-3,5-dihydroxy-6-methyloxan-2-yl][hydroxy-[[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy]phosphoryl] hydrogen phosphate: F44 (≠ D71), E46 (≠ G73), D84 (≠ A113), Y92 (≠ A121)

7s45A Crystal structure of an n-acetyltransferase, c80t mutant, from helicobacter pullorum in the presence of acetyl coenzyme a and dtdp (see paper)
32% identity, 55% coverage: 69:168/182 of query aligns to 42:131/141 of 7s45A

query
sites
7s45A

H

H

C

C

D
x
F

Y

I

G
x
E

Y

N

N

D

I

V

H

K

L

I

G

G

E

N

G

N

A

V

F

T

L

I

N

K

F

C

N

G

C

V

V

Q

I

I

L
x
W

D

D

V

G

V

I

E

E

V

I

H

E

I

-

G

-

A

D

G

D

A

V

Q

F

I

I

G

G

P

P

A

N

V

V

Q

T

L

F

Y
x
T

T
x
N

A
x
D

D
x
K

H
x
Y

P
|
P

R

R

D

S

P

K

E

Q

A

F

R

S

R

K

S

T

G

I

V

I

E

K

F

K

G

G

R

A

P

S

I

I

N

-

I

-

G

-

R

-

N

-

V

-

W

-

V

-

G

G

G

A

G

N

A

A

I

T

I

I

L
|
L

P

P

G

G

V

I

T

T

I

I

G

G

D

E

D

N

A

A

V

M

I

I

G

G

A

A

G

G

S

A

V

I

V

V

T
|
T

R
x
K

D

D

V

V

binding acetyl coenzyme *a: W64 (≠ L91), T82 (≠ Y111), N83 (≠ T112), D84 (≠ A113), K85 (≠ D114), Y86 (≠ H115), P87 (= P116), L110 (= L147), T128 (= T165), K129 (≠ R166)
binding thymidine-5'-diphosphate: F44 (≠ D71), E46 (≠ G73)

4eaaA X-ray crystal structure of the h141n mutant of perosamine n- acetyltransferase from caulobacter crescentus in complex with coa and gdp-perosamine (see paper)
31% identity, 58% coverage: 78:182/182 of query aligns to 124:208/210 of 4eaaA

query
sites
4eaaA

L

I

G

G

E

D

G

L

A

A

F

I

L

I

N

N

F

T

N

G

C

A

V

V

I

V

L

D

D

N

V

-

E

D

V

C

H

R

I

L

G

G

A

A

G

A

A

C

Q

H

I

L

G

G

P
|
P

A

A

V

S

Q

A

L

L

Y

A

T

G

A

G

D

-

H

-

P

-

R

-

D

-

P

-

E

-

A

-

R

-

S

-

G

-

V

-

E

-

F

-

G

-

R

-

P

-

I

V

N

S

I

V

G

G

R

E

N

R

V

A

W
x
F

V

L

G

G

G
x
V

G

G

A

A

I

R

I

V

L

I

P

P

G

G

V

V

T

T

I

I

G

G

D

A

D

D

A

T

V

I

I

V

G

G

A
|
A

G

G

S

G

V

V

T

V

R

R

D

D

V

L

P

P

A

D

G

S

A

V

T

L

V

A

V
x
I

G

G

N

V

P

P

A

A

R

K

I

I

R
x
K

binding coenzyme a: P150 (= P106), F165 (≠ W139), V168 (≠ G142), A186 (= A160), I201 (≠ V175), K208 (≠ R182)

Sites not aligning to the query:

binding GDP-perosamine: 14, 15, 16, 35, 36, 37, 52, 70, 71

3fscA Crystal structure of qdtc, the dtdp-3-amino-3,6-dideoxy-d-glucose n- acetyl transferase from thermoanaerobacterium thermosaccharolyticum in complex with coa and dtdp-3-amino-fucose (see paper)
32% identity, 59% coverage: 73:180/182 of query aligns to 108:217/259 of 3fscA

query
sites
3fscA

G

G

Y

N

N

N

I

V

H
x
K

L

I

G
|
G

E
x
T

G

L

A

S

F

D

L

I

N

Q

F

H

N

H

C

V

V

Y

I

I

L

G

D

N

V

Y

V

V

E

N

V

I

H
|
H

-
x
S

-

N

-

V

-

F

I

V

G

G

A

E

G

K

A

S

Q

I

I

I

G

K

P

D

A

F

V

V

Q

W

L

L

Y
x
F

-

P

-

H

-

V

T

L

A

T

D

N

H

D

P

P

R

T

D

P

P

P

E

S

A

N

R

E

R

L

S

L

G

G

V

V

E

-

F

-

G

-

R

-

P

-

I

-

N

T

I

I

G

E

R

L

N

F

V

A

W

V

V

I

G
x
A

G

R

A

S

I

V

L

L

P

P

G

G

V

I

T

H

I

I

G

N

D

E

D

D

A

A

V

L

I

V

G
|
G

A
|
A

G

G

S

A

V

V

T

T

R

K

D

D

V

V

P

P

A

K

G

E

A

T

T

V

V

V

V
|
V

G

G

N

N

P

P

A

A

R

R

binding coenzyme a: F150 (≠ Y111), A178 (≠ G141), A179 (≠ G142), G196 (= G159), A197 (= A160), V212 (= V175)
binding (3R,4S,5R,6R)-4-amino-3,5-dihydroxy-6-methyloxan-2-yl][hydroxy-[[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy]phosphoryl] hydrogen phosphate: K112 (≠ H77), G114 (= G79), T115 (≠ E80), H132 (= H97), S133 (vs. gap)

Sites not aligning to the query:

Query Sequence

>GFF855 FitnessBrowser__psRCH2:GFF855
MAMSEKQKMLAGELYYPGDPEILADQAAAKAWMVRYNAALAASPDERRALLAERLASVGA
GAVIRPPFHCDYGYNIHLGEGAFLNFNCVILDVVEVHIGAGAQIGPAVQLYTADHPRDPE
ARRSGVEFGRPINIGRNVWVGGGAIILPGVTIGDDAVIGAGSVVTRDVPAGATVVGNPAR
IR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory