SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF891 FitnessBrowser__Phaeo:GFF891 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

O59954 Adenylyltransferase and sulfurtransferase uba4; Common component for nitrate reductase and xanthine dehydrogenase protein F; Ubiquitin-like protein activator 4; EC 2.7.7.80; EC 2.8.1.11 from Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) (see paper)
44% identity, 61% coverage: 10:209/329 of query aligns to 68:278/482 of O59954

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O59954

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G82 (= G24) mutation to D: In cnxF21ts and cnxF24ts; temperature-sensitive mutant. Impairs molybdopterin biosynthesis.
G100 (= G42) mutation to S: In cnxF1285; impairs molybdopterin biosynthesis.
R130 (= R72) mutation to Q: In cnxF200; impairs molybdopterin biosynthesis.
C185 (≠ A127) mutation to Y: In cnxF472; impairs molybdopterin biosynthesis.
E215 (≠ S157) mutation to K: In cnxF119; impairs molybdopterin biosynthesis.
G264 (= G195) mutation to S: In cnxF142ts; temperature-sensitive mutant. Impairs molybdopterin biosynthesis.

Q9VLJ8 Adenylyltransferase and sulfurtransferase MOCS3; Molybdenum cofactor synthesis protein 3; Ubiquitin activating enzyme 4; EC 2.7.7.80; EC 2.8.1.11 from Drosophila melanogaster (Fruit fly) (see paper)
39% identity, 66% coverage: 8:224/329 of query aligns to 69:288/453 of Q9VLJ8

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Sites not aligning to the query:

62 modified: Phosphothreonine

6yubB Crystal structure of uba4 from chaetomium thermophilum (see paper)
40% identity, 65% coverage: 8:222/329 of query aligns to 12:227/289 of 6yubB

query
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binding zinc ion: C184 (≠ A181), C187 (= C184)

Sites not aligning to the query:

binding zinc ion: 265

6yubA Crystal structure of uba4 from chaetomium thermophilum (see paper)
39% identity, 65% coverage: 8:222/329 of query aligns to 11:228/423 of 6yubA

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binding zinc ion: C183 (≠ A181), C186 (= C184)

Sites not aligning to the query:

binding zinc ion: 266, 269

P38820 Adenylyltransferase and sulfurtransferase UBA4; Needs CLA4 to survive protein 3; Ubiquitin-like protein activator 4; EC 2.7.7.-; EC 2.8.1.- from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 4 papers)
36% identity, 63% coverage: 5:210/329 of query aligns to 41:251/440 of P38820

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P38820

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C225 (= C184) mutation C->A,S: Abolishes adenylyltransferase activity but not sulfurtransferase activity.

Sites not aligning to the query:

397 mutation C->A,S: Abolishes sulfurtransferase activity but not adenylyltransferase activity.

P12282 Molybdopterin-synthase adenylyltransferase; MoaD protein adenylase; Molybdopterin-converting factor subunit 1 adenylase; Sulfur carrier protein MoaD adenylyltransferase; EC 2.7.7.80 from Escherichia coli (strain K12) (see 2 papers)
37% identity, 66% coverage: 8:224/329 of query aligns to 9:227/249 of P12282

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P12282

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x
C

A

T

D
|
D

S

N

H

V

A

A

V

V

S

R

Y

N

T

Q

L

L

S

N

D

A

C

G

C
|
C

Q

F

R

A

L

A

G

K

R

V

D

P

L

L

I

V

S

S

G

G

S

A

A

A

L

I

A

R

Q

M

S

E

G

G

Y

Q

V

I

G

T

V

V

F

F

C

T

-

Y

-

Q

G

D

G

G

G

E

P

P

S
x
C

L

Y

R

R

A
x
C

V

L

F

S

P

R

D

L

P

F

P

G

G

E

S

N

A

A

A

L

T

T

C
|
C

A

V

T

E

S

A

G

G

I

V

M

M

G

A

P

P

V

L

V

I

G

G

V

V

I

I

G

G

A

S

W

L

Q

Q

A

A

Q

M

L

E

A

A

L

I

K

K

L

M

L

L

L

A

N

G

H

Y

Q

G

P

K

T

P

P

A

R

S

G

G

R

K

I

I

F

V

S

M

V

Y

D

D

R14 (= R13) mutation R->A,K: No effect.; mutation to A: No activity; when associated with A-73.
C44 (≠ S43) mutation to A: No effect.
R73 (= R72) mutation to A: No effect. No activity; when associated with A-14.; mutation to K: Substantially reduced activity.
C128 (≠ A127) mutation to A: No effect.; mutation to Y: No activity.
D130 (= D129) mutation to A: No activity.; mutation to E: Substantially reduced activity.
C142 (= C141) mutation to A: No effect.
C172 (≠ S169) mutation to A: No zinc bound and no enzyme activity.
C175 (≠ A172) mutation to A: No zinc bound and no enzyme activity.
C187 (= C184) mutation to A: No effect.

Sites not aligning to the query:

231 C→A: No effect.
244 C→A: No zinc bound and almost no enzyme activity.
247 C→A: No zinc bound and almost no enzyme activity.

O95396 Adenylyltransferase and sulfurtransferase MOCS3; Molybdenum cofactor synthesis protein 3; Molybdopterin synthase sulfurylase; MPT synthase sulfurylase; EC 2.7.7.80; EC 2.8.1.11 from Homo sapiens (Human) (see 4 papers)
39% identity, 65% coverage: 10:224/329 of query aligns to 62:279/460 of O95396

query
sites
O95396

R

R

F

Y

D

S

R

R

Q

Q

I

L

A

V

L

L

P

P

E

E

V

L

G

G

A

V

E

H

G

G

Q

Q

A

L

K

R

L

L

A

G

A

T

A

A

H

C

V

V

L

L

V

I

V

V

G

G

A

C

G

G

L

L

G

G

S

C

P

P

V

L

L

A

Q

Q

Y

Y

L

L

A

A

G

A

A

A

G

G

I

V

G

G

E

R

I

L

T

G

V

L

M

V

D

D

G

Y

D

D

S

V

V

V

E

E

A

M

T

S

N

N

L

L

P

A

R

R

Q

Q

P

V

L

L

Y

H

T

G

P

E

A

A

D

L

I

A

G

G

R

Q

Y

A

K

K

V

A

D

F

A

S

A

A

R

A

E

A

R

S

L

L

C

R

A

R

M

L

A

N

P

S

E

A

L

V

R

E

L

C

H

V

P

P

H

Y

A

T

R

Q

D

A

L

L

T

T

P

P

D

A

N

T

V

A

M

L

A

D

A

L

V

V

T

R

P

R

V

Y

D

D

L

V

V

V

I

A

D

D

A

C

A

S

D

D

S

N

H

V

A

P

V

T

S

R

Y

Y

T

L

L

V

S

N

D

D

C

A

C

C

Q

V

R

L

L

A

G

G

R

R

D

P

L

L

I

V

S

S

G

A

S

S

A

A

L

L

A

R

Q

F

S

E

G

G

Y

Q

V

I

G

T

V

V

F

Y

-

H

C

Y

G

D

G

G

P

P

S

C

L

Y

R

R

A

C

V

I

F

F

P

P

D

Q

P

P

-

P

-

A

G

E

S

T

A

V

A

T

T

N

C
|
C

A

A

T

D

S

G

G

G

I

V

M

L

G

G

P

V

V

V

V

T

G

G

V

V

I

L

G

G

A

C

W

L

Q

Q

A

A

Q

L

L

E

A

V

L

L

K

K

L

I

L

A

N

G

H

L

Q

G

P

P

T

S

P

Y

R

S

G

G

R

S

I

L

F

L

S

L

V

F

D

D

C239 (= C184) mutation to A: Impairs sulfurtransferase activity.

Sites not aligning to the query:

316 modified: Disulfide link with 324; C→A: Does not affect sulfurtransferase activity.
324 modified: Disulfide link with 316; C→A: Does not affect sulfurtransferase activity.
365 C→A: Does not affect sulfurtransferase activity.
412 active site, Cysteine persulfide intermediate; for sulfurtransferase activity; C→A: Abolishes sulfurtransferase activity.
413 K→R: Does not affect sulfurtransferase specificity and activity.
414 L→K: Does not affect sulfurtransferase specificity and activity.
415 G→A: Does not affect sulfurtransferase specificity and activity.
416 N→V: Does not affect sulfurtransferase specificity and activity.
417 D→R: Results in 470-fold increased activity.; D→T: Results in 90-fold increased activity.
458 P→G: Does not affect sulfurtransferase specificity and activity.
460 Y→A: Does not affect sulfurtransferase specificity and activity.

Q72J02 Sulfur carrier protein adenylyltransferase; E1-like protein TtuC; Sulfur carrier protein MoaD adenylyltransferase; Sulfur carrier protein ThiS adenylyltransferase; Sulfur carrier protein TtuB adenylyltransferase; tRNA two-thiouridine-synthesizing protein C; EC 2.7.7.80; EC 2.7.7.73; EC 2.7.7.- from Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) (see paper)
43% identity, 62% coverage: 8:210/329 of query aligns to 8:218/271 of Q72J02

query
sites
Q72J02

M

L

S

D

R

R

F

Y

D

H

R

R

Q

Q

I

M

A

I

L

L

P

P

E

Q

V

V

G

G

A

P

E

E

G

G

Q

Q

A

E

K

R

L

L

A

K

A

R

A

A

H

S

V

V

L

V

V

V

G

G

A

A

G

G

L

L

G

G

S

V

P

P

V

V

L

L

Q

Q

Y

Y

L

L

A

V

G

A

A

A

G

G

I

V

G

G

E

R

I

V

T

G

V

V

M

V

D

E

G

M

D

D

S

R

V

V

E

E

A

V

T

S

N

N

L

L

P

H

R

R

Q

Q

P

V

L

L

Y

Y

T

T

P

T

A

E

D

D

I

V

G

G

R

E

Y

P

K

K

V

A

D

L

A

V

A

A

R

Q

E

K

R

R

L

L

C

Q

A

A

M

L

A

N

P

P

E

L

L

V

R

R

L

V

H

E

P

A

H

Y

A

P

R

V

D

R

L

L

T

T

P

S

D

E

N

N

V

A

M

L

A

E

A

I

V

L

T

R

P

P

V

Y

D

D

L

L

V

V

I

V

D

D

A

A

A

S

D

D

S

N

H

F

A

P

V

T

S

R

Y

Y

T

L

L

V

S

N

D

D

C

A

Q

V

R

L

L

L

G

G

R

K

D

P

L

L

I

V

S

F

G

G

S

A

A

I

L

Y

A

Q

Q

F

S

D

G

G

Y

Q

V

V

G

A

V

V

F

F

C

H

G

H

-

P

-

T

-

L

-

H

-

G

-

E

G

M

G

G

P

P

S

C

L

Y

R

R

A

C

V

L

F

F

-

P

-

K

P

P

D

P

P

P

G

G

S

A

A

V

A

P

T

S

C
|
C

A

A

T

E

S

A

G

G

I

V

M

F

G

G

P

V

V

L

V

P

G

A

V

V

I

V

G

G

A

S

W

L

Q

M

A

A

Q

A

L

E

A

A

L

L

K

K

L

V

L

L

C192 (= C184) modified: Glycyl cysteine thioester (Cys-Gly) (interchain with G-Cter in TtuB); mutation to S: Not able to form a thioester complex with TtuB.

Sites not aligning to the query:

268 C→S: Still able to form a thioester complex with TtuB.

D4GSF3 SAMP-activating enzyme E1; Ubiquitin-like activating enzyme of archaea; Ubl-activating enzyme; EC 2.7.7.- from Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii) (see paper)
35% identity, 77% coverage: 8:259/329 of query aligns to 10:262/270 of D4GSF3

query
sites
D4GSF3

M

L

S

D

R

R

F

Y

D

S

R

R

Q

H

I

I

A

I

L

M

P

D

E

E

V

V

G

G

A

P

E

E

G

G

Q

Q

A

G

K

R

L

L

A

L

A

S

H

R

V

V

L

V

V

V

G

G

A

A

G

G

L

L

G

G

S

A

P

P

V

A

L

I

Q

Q

Y

Y

L

L

A

A

G

A

A

V

G

G

I

V

G

G

E

E

I

L

T

V

V

V

M

V

D

D

G

D

D

D

S

V

V

V

E

E

A

R

T

S

N

N

L

L

P

Q

R

R

Q

Q

P

V

L

V

Y

H

T

C

P

D

A

D

D

D

I

V

G

G

R

T

Y

P

K

K

V

A

D

E

A

S

A

A

R

A

E

A

R

F

L

V

C

R

A

G

M

L

A

N

P

P

E

D

L

V

R

S

L

V

H

E

P

P

H

V

A

E

R

A

D

R

L

V

T

D

P

K

D

S

N

N

V

V

M

H

A

E

A

V

V

V

T

A

P

G

V

S

D

D

L

V

V

V

I

V

D

D

A

A

A

S

D

D

S

N

H

F

A

P

V

T

S

R

Y

Y

T

L

L

L

S

N

D

D

C

V

C

C

Q

R

R

F

L

E

G

G

R

I

D

P

L

L

I

V

S

H

G

G

S

A

A

I

L

Y

A

K

Q

F

S

E

G

G

Y

Q

V

A

G

T

V

T

F

L

C

V

G

P

G

D

G

G

P

P

S

C

L

Y

R

R

A

C

V

L

F

F

P

P

D

E

-

A

-

P

P

E

P

P

G

G

S

T

A

V

A

P

T

D

C
|
C

A

A

T

T

S

T

G

G

I

V

M

L

G

G

P

V

V

L

V

P

G

G

V

T

I

V

G

G

A

C

W

I

Q

Q

A

A

Q

T

L

E

A

A

L

M

K

K

L

L

N

D

H

E

Q

G

P

E

T

A

P

L

R

D

G

G

R

R

I

L

F

L

S

F

V

Y

D

D

F

-

A

-

G

-

L

-

Q

-

M

-

G

-

G

A

F

M

D

D

F

M

S

T

T

F

A

E

R

T

E

V

P

P

A

Y

R

R

P

T

L

N

P

P

F

D

I

C

-

P

-

V

-

C

-

G

-

E

-

G

G

G

L

V

N

D

A

S

I

I

T

E

D

D

E

I

D

D

Y

Y

V

V

C188 (= C184) mutation to S: Loss of activity since this mutant is not able to complement a ubaA deletion in trans to restore sampylation and tRNA thiolation.

1jwbB Structure of the covalent acyl-adenylate form of the moeb-moad protein complex (see paper)
36% identity, 66% coverage: 8:224/329 of query aligns to 8:219/240 of 1jwbB

query
sites
1jwbB

M

M

S

L

R

R

F

Y

D

N

R
|
R

Q

Q

I

I

A

I

L

L

P

R

E

G

V

F

G

D

A

F

E

D

G

G

Q

Q

A

E

K

A

L

L

A

K

A

D

A

S

H

R

V

V

L

L

V

I

V
|
V

G
|
G

A
x
L

G
|
G

L

L

G

G

S

C

P

A

V

A

L

S

Q

Q

Y

Y

L

L

A

A

G

S

A

A

G

G

I

V

G

G

E

N

I

L

T

T

V

L

M
x
L

D
|
D

G
x
F

D
|
D

S

T

V

V

E

S

A

L

T

S

N

N

L

L

P

Q

R
|
R

Q

Q

P

T

L

L

Y

H

T

S

P

D

A

A

D

T

I

V

G

G

R

Q

Y

P

K
|
K

V

V

D

E

A

S

A

A

R

R

E

D

R

A

L

L

C

T

A

R

M

I

A

N

P

P

E

H

L

I

R

A

L

I

H

T

P

P

H

V

A

N

R

A

D
x
L

L

L

T

D

P

D

D

A

N

E

V

L

M

A

A

A

A

L

V

I

T

A

P

E

V

H

D

D

L

L

V

V

I

L

D

D

A
x
C

A
x
T

D
|
D

S
x
N

H

V

A

A

V
|
V

S

R

Y

N

T

Q

L

L

S

N

D

A

C

G

C

C

Q

F

R

A

L

A

G

K

R

V

D

P

L

L

I

V

S

S

G

G

S

A

A

A

L

I

A

R

Q

M

S

E

G

G

Y

Q

V

I

G

T

V

V

F

F

C

T

-

Y

-

Q

G

D

G

G

G

E

P

P

S
x
C

L

Y

R

R

A
x
C

V

L

F

-

P

-

D

-

P

-

G

-

S

-

A

S

A

R

T

L

C

F

A

G

T

E

S

A

G

G

I

V

M

M

G

A

P

P

V

L

V

I

G

G

V

V

I

I

G

G

A

S

W

L

Q

Q

A

A

Q

M

L

E

A

A

L

I

K

K

L

M

L

L

L

A

N

G

H

Y

Q

G

P

K

T

P

P

A

R

S

G

G

R

K

I

I

F

V

S

M

V

Y

D

D

active site: R13 (= R13), D129 (= D129)
binding adenosine monophosphate: V36 (= V36), G37 (= G37), L38 (≠ A38), G39 (= G39), G40 (= G40), L60 (≠ M60), D61 (= D61), F62 (≠ G62), D63 (= D63), R72 (= R72), K85 (= K85), L108 (≠ D108), C127 (≠ A127), T128 (≠ A128), D129 (= D129), N130 (≠ S130), V133 (= V133)
binding zinc ion: C171 (≠ S169), C174 (≠ A172)

Sites not aligning to the query:

binding zinc ion: 236, 239

1jw9B Structure of the native moeb-moad protein complex (see paper)
36% identity, 66% coverage: 8:224/329 of query aligns to 8:219/240 of 1jw9B

query
sites
1jw9B

M

M

S

L

R

R

F

Y

D

N

R
|
R

Q

Q

I

I

A

I

L

L

P

R

E

G

V

F

G

D

A

F

E

D

G

G

Q

Q

A

E

K

A

L

L

A

K

A

D

A

S

H

R

V

V

L

L

V

I

V

V

G

G

A

L

G

G

L

L

G

G

S

C

P

A

V

A

L

S

Q

Q

Y

Y

L

L

A

A

G

S

A

A

G

G

I

V

G

G

E

N

I

L

T

T

V

L

M

L

D

D

G

F

D

D

S

T

V

V

E

S

A

L

T

S

N

N

L

L

P

Q

R

R

Q

Q

P

T

L

L

Y

H

T

S

P

D

A

A

D

T

I

V

G

G

R

Q

Y

P

K

K

V

V

D

E

A

S

A

A

R

R

E

D

R

A

L

L

C

T

A

R

M

I

A

N

P

P

E

H

L

I

R

A

L

I

H

T

P

P

H

V

A

N

R

A

D

L

L

L

T

D

P

D

D

A

N

E

V

L

M

A

A

A

A

L

V

I

T

A

P

E

V

H

D

D

L

L

V

V

I

L

D

D

A

C

A

T

D
|
D

S

N

H

V

A

A

V

V

S

R

Y

N

T

Q

L

L

S

N

D

A

C

G

C

C

Q

F

R

A

L

A

G

K

R

V

D

P

L

L

I

V

S

S

G

G

S

A

A

A

L

I

A

R

Q

M

S

E

G

G

Y

Q

V

I

G

T

V

V

F

F

C

T

-

Y

-

Q

G

D

G

G

G

E

P

P

S
x
C

L

Y

R

R

A
x
C

V

L

F

-

P

-

D

-

P

-

G

-

S

-

A

S

A

R

T

L

C

F

A

G

T

E

S

A

G

G

I

V

M

M

G

A

P

P

V

L

V

I

G

G

V

V

I

I

G

G

A

S

W

L

Q

Q

A

A

Q

M

L

E

A

A

L

I

K

K

L

M

L

L

L

A

N

G

H

Y

Q

G

P

K

T

P

P

A

R

S

G

G

R

K

I

I

F

V

S

M

V

Y

D

D

active site: R13 (= R13), D129 (= D129)
binding zinc ion: C171 (≠ S169), C174 (≠ A172)

Sites not aligning to the query:

binding zinc ion: 236, 239

1jwaB Structure of the atp-bound moeb-moad protein complex (see paper)
35% identity, 66% coverage: 8:224/329 of query aligns to 8:204/217 of 1jwaB

query
sites
1jwaB

M

M

S

L

R

R

F

Y

D

N

R
|
R

Q

Q

I

I

A

I

L

L

P

R

E

G

V

F

G

D

A

F

E

D

G

G

Q

Q

A

E

K

A

L

L

A

K

A

D

A

S

H

R

V

V

L

L

V

I

V
|
V

G
|
G

A

L

G
|
G

L

L

G

G

S

C

P

A

V

A

L

S

Q

Q

Y

Y

L

L

A

A

G

S

A

A

G

G

I

V

G

G

E

N

I

L

T

T

V

L

M
x
L

D
|
D

G
x
F

D
|
D

S

T

V

V

E

S

A

L

T

S

N
|
N

L

L

P

Q

R
|
R

Q
|
Q

P

T

L

L

Y

H

T

S

P

D

A

A

D

T

I

V

G

G

R

Q

Y

P

K
|
K

V

V

D

E

A

S

A

A

R

R

E

D

R

A

L

L

C

T

A

R

M

I

A

N

P

P

E

H

L

I

R

A

L

I

H

T

P

P

H

V

A

N

R

A

D
x
L

L

L

T

D

P

D

D

A

N

E

V

L

M

A

A

A

A

L

V

I

T

A

P

E

V

H

D

D

L

L

V

V

I

L

D

D

A
x
C

A
x
T

D
|
D

S
x
N

H

V

A

A

V
|
V

S

R

Y

N

T

Q

L

L

S

N

D

A

C

G

C

C

Q

F

R

A

L

A

G

K

R

V

D

P

L

L

I

V

S

S

G

G

S

A

A

A

L

I

A

R

Q

M

S

E

G

G

Y

Q

V

I

G

T

V

V

F

F

C

-

G

-

P

-

S

-

L

-

R

-

A

-

V

-

F

-

P

-

D

-

P

-

G

-

S

-

A

-

T

-

C

T

A

Y

T

E

S

A

G

G

I

V

M

M

G

A

P

P

V

L

V

I

G

G

V

V

I

I

G

G

A

S

W

L

Q

Q

A

A

Q

M

L

E

A

A

L

I

K

K

L

M

L

L

L

A

N

G

H

Y

Q

G

P

K

T

P

P

A

R

S

G

G

R

K

I

I

F

V

S

M

V

Y

D

D

active site: R13 (= R13), D129 (= D129)
binding adenosine-5'-triphosphate: V36 (= V36), G37 (= G37), G39 (= G39), G40 (= G40), L60 (≠ M60), D61 (= D61), F62 (≠ G62), D63 (= D63), N69 (= N69), R72 (= R72), Q73 (= Q73), K85 (= K85), L108 (≠ D108), C127 (≠ A127), T128 (≠ A128), D129 (= D129), N130 (≠ S130), V133 (= V133)

1zfnA Structural analysis of escherichia coli thif (see paper)
37% identity, 67% coverage: 6:224/329 of query aligns to 4:223/244 of 1zfnA

query
sites
1zfnA

R

R

L

D

M

F

S

M

R

R

F

Y

D

S

R
|
R

Q

Q

I

I

A

L

L

L

P

D

E

D

V

I

G

A

A

L

E

D

G

G

Q

Q

A

Q

K

K

L

L

A

L

A

D

A

S

H

Q

V

V

L

L

V

I

V
x
I

G
|
G

A
x
L

G
|
G

L

L

G

G

S

T

P

P

V

A

L

A

Q

L

Y

Y

L

L

A

A

G

G

A

A

G

G

I

V

G

G

E

T

I

L

T

V

V

L

M
x
A

D
|
D

G

D

D
|
D

S

D

V

V

E

H

A

L

T

S

N
|
N

L

L

P

Q

R
|
R

Q
|
Q

P

I

L

L

Y

F

T

T

P

T

A

E

D

D

I

I

G

D

R

R

Y

P

K
|
K

V

S

D

Q

A

V

A

S

R

Q

E

Q

R

R

L

L

C

T

A

Q

M

L

A

N

P

P

E

D

L

I

R

Q

L

L

H

T

P

A

H

L

A

Q

R
x
Q

D
x
R

L
|
L

T

T

P

G

D

E

N

A

V

L

M

K

A

D

A

A

V

V

T

A

P

R

V

A

D

D

L

V

V

V

I

L

D

D

A
x
C

A
x
T

D
|
D

S

N

H

M

A

A

V
x
T

S

R

Y

Q

T

E

L

I

S

N

D

A

C

A

C

C

Q

V

R

A

L

L

G

N

R

T

D

P

L

L

I

I

S

T

G

A

S

S

A

A

L

V

A

G

Q

F

S

G

G

G

Y

Q

V

L

G

M

V

V

F

L

C

T

-

P

G

W

G

E

G

Q

P

G

S
x
C

L

Y

R

R

A
x
C

V

L

F

W

P

P

D

D

P

N

P

Q

G

E

S

P

A

E

A

R

T

N

C

C

A

R

T

T

S

A

G

G

I

V

M

V

G

G

P

P

V

V

G

G

V

V

I

M

G

G

A

T

W

L

Q

Q

A

A

Q

L

L

E

A

A

L

I

K

K

L

L

L

S

N

G

H

I

Q

E

P

-

T

T

P

P

R

A

G

G

R

E

I

L

F

R

S

L

V

F

D

D

active site: R11 (= R13), D127 (= D129)
binding adenosine-5'-triphosphate: I34 (≠ V36), G35 (= G37), L36 (≠ A38), G37 (= G39), G38 (= G40), A58 (≠ M60), D59 (= D61), D61 (= D63), N67 (= N69), R70 (= R72), Q71 (= Q73), K83 (= K85), Q105 (≠ R107), R106 (≠ D108), L107 (= L109), C125 (≠ A127), T126 (≠ A128), D127 (= D129), T131 (≠ V133)
binding zinc ion: C169 (≠ S169), C172 (≠ A172)

Sites not aligning to the query:

binding zinc ion: 240, 243

P30138 Sulfur carrier protein ThiS adenylyltransferase; EC 2.7.7.73 from Escherichia coli (strain K12) (see 3 papers)
37% identity, 67% coverage: 6:224/329 of query aligns to 4:223/251 of P30138

query
sites
P30138

R

R

L

D

M

F

S

M

R

R

F

Y

D

S

R

R

Q

Q

I

I

A

L

L

L

P

D

E

D

V

I

G

A

A

L

E

D

G

G

Q

Q

A

Q

K

K

L

L

A

L

A

D

A

S

H

Q

V

V

L

L

V

I

G

G

A

L

G

G

L

L

G

G

S

T

P

P

V

A

L

A

Q

L

Y

Y

L

L

A

A

G

G

A

A

G

G

I

V

G

G

E

T

I

L

T

V

V

L

M

A

D

D

G

D

D

D

S

D

V

V

E

H

A

L

T

S

N

N

L

L

P

Q

R

R

Q

Q

P

I

L

L

Y

F

T

T

P

T

A

E

D

D

I

I

G

D

R

R

Y

P

K

K

V

S

D

Q

A

V

A

S

R

Q

E

Q

R

R

L

L

C

T

A

Q

M

L

A

N

P

P

E

D

L

I

R

Q

L

L

H

T

P

A

H

L

A

Q

R

Q

D

R

L

L

T

T

P

G

D

E

N

A

V

L

M

K

A

D

A

A

V

V

T

A

P

R

V

A

D

D

L

V

V

V

I

L

D

D

A

C

A

T

D

D

S

N

H

M

A

A

V

T

S

R

Y

Q

T

E

L

I

S

N

D

A

C

A

C

C

Q

V

R

A

L

L

G

N

R

T

D

P

L

L

I

I

S

T

G

A

S

S

A

A

L

V

A

G

Q

F

S

G

G

G

Y

Q

V

L

G

M

V

V

F

L

C

T

-

P

G

W

G

E

G

Q

P

G

S
x
C

L

Y

R

R

A
x
C

V

L

F
x
W

P

P

D

D

P

N

P

Q

G

E

S

P

A

E

A

R

T

N

C
|
C

A

R

T

T

S

A

G

G

I

V

M

V

G

G

P

P

V

V

G

G

V

V

I

M

G

G

A

T

W

L

Q

Q

A

A

Q

L

L

E

A

A

L

I

K

K

L

L

L

S

N

G

H

I

Q

E

P

-

T

T

P

P

R

A

G

G

R

E

I

L

F

R

S

L

V

F

D

D

C169 (≠ S169) binding
C172 (≠ A172) binding
W174 (≠ F174) mutation to A: No adenylation of ThiS.
C184 (= C184) mutation to S: No cross-link formed with ThiS. No effect on ThiS thiocarboxylate formation in vitro. Does not support growth.

Sites not aligning to the query:

240 binding
243 binding

1zud3 Structure of this-thif protein complex (see paper)
37% identity, 48% coverage: 6:163/329 of query aligns to 4:161/240 of 1zud3

query
sites
1zud3

R

R

L

D

M

F

S

M

R

R

F

Y

D

S

R
|
R

Q

Q

I

I

A
x
L

L

L

P
x
D

E

D

V

I

G

A

A

L

E

D

G

G

Q

Q

A

Q

K

K

L

L

A

L

A

D

A

S

H

Q

V

V

L

L

V

I

G

G

A

L

G

G

L

L

G

G

S

T

P

P

V

A

L

A

Q

L

Y

Y

L

L

A

A

G

G

A

A

G

G

I

V

G

G

E

T

I

L

T

V

V

L

M

A

D

D

G

D

D

D

S

D

V

V

E

H

A

L

T

S

N

N

L

L

P

Q

R

R

Q

Q

P

I

L

L

Y

F

T

T

P

T

A

E

D

D

I

I

G

D

R

R

Y

P

K

K

V

S

D

Q

A

V

A

S

R

Q

E

Q

R

R

L

L

C

T

A

Q

M

L

A

N

P

P

E

D

L

I

R

Q

L

L

H

T

P

A

H

L

A

Q

R

Q

D

R

L

L

T

T

P

G

D

E

N

A

V

L

M

K

A

D

A

A

V

V

T

A

P

R

V

A

D

D

L

V

V

V

I

L

D

D

A

C

A

T

D
|
D

S

N

H

M

A

A

V

T

S

R

Y

Q

T

E

L

I

S

N

D

A

C

A

C

C

Q

V

R

A

L

L

G

N

R

T

D

P

L

L

I

I

S

T

G

A

S

S

A

A

L

V

A

G

Q

F

S

G

G

G

Y

Q

V

L

G

M

V

V

F

L

active site: R11 (= R13), D127 (= D129)
binding calcium ion: L14 (≠ A16), D16 (≠ P18)

Sites not aligning to the query:

binding zinc ion: 169, 172, 235, 238

6nyaA Crystal structure of ubiquitin e1 (uba1) in complex with ubc3 (cdc34) and ubiquitin (see paper)
29% identity, 60% coverage: 9:204/329 of query aligns to 405:609/987 of 6nyaA

query
sites
6nyaA

S

S

R

R

F

Y

D

D

R

N

Q

Q

I

I

A

A

L

V

P

-

E

-

V

F

G

G

A

L

E

D

G

F

Q

Q

A

K

K

K

L

I

A

A

A

N

A

S

H

K

V

V

L

F

V

L

V

V

G
|
G

A

S

G
|
G

G
x
A

L

I

G

G

S

C

P

E

V

M

L

L

Q

K

Y

N

L

W

A

A

G

L

A

L

G

G

I

L

G

G

E

S

-

G

-

S

-

D

-

G

-

Y

I

I

T

V

V

V

M
x
T

D
|
D

G
x
N

D
|
D

S

S

V

I

E

E

A

K

T

S

N
|
N

L

L

P

N

R
|
R

Q
|
Q

P

F

L

L

Y

F

T

R

P

P

A

K

D

D

I

V

G

G

R

K

Y

N

K
|
K

V

S

D

E

A

V

A

A

R

A

E

E

R

A

L

V

C

C

A

A

M

M

A

N

P

P

E

D

L

L

-

K

-

G

-

K

-

I

-

N

-

A

-

K

-

I

-

D

R
x
K

L
x
V

H

G

P

P

H

E

A

T

R

E

D

E

L

I

T

F

P

N

D

D

N

S

V

F

M

W

A

E

A

S

V

-

T

-

P

-

V

L

D

D

L

F

V

V

I

T

D

N

A
|
A

A
x
L

D
|
D

S
x
N

H

V

A

D

V
x
A

S

R

Y

T

T

Y

L

V

S

D

D

R

C

R

C

C

Q

V

R

F

L

Y

G

R

R

K

D

P

L

L

I

L

S

E

G

S

S

G

A

T

L

L

A

G

Q

T

S

K

G

G

Y

N

V

T

G

Q

V

V

F

I

C

I

G

-

P

P

S

R

L

L

R

T

A

E

V

S

F

Y

P

S

-

S

-

R

D

D

P

P

-

E

G

K

S

S

A

I

A

P

T

L

C
|
C

A
x
T

T

L

S
x
R

G

S

I

F

M

P

G

N

P

K

V

I

V

D

G

H

V

T

I

I

G

-

A

A

W

W

Q

A

A

K

Q

S

L

L

active site: R471 (= R72), D534 (= D129), C590 (= C184), T591 (≠ A185), R593 (≠ S187)
binding adenosine-5'-triphosphate: G431 (= G37), G433 (= G39), A434 (≠ G40), T459 (≠ M60), D460 (= D61), N461 (≠ G62), D462 (= D63), N468 (= N69), R471 (= R72), Q472 (= Q73), K484 (= K85), K509 (≠ R101), V510 (≠ L102), A532 (= A127), L533 (≠ A128), D534 (= D129), N535 (≠ S130), A538 (≠ V133)

Sites not aligning to the query:

active site: 11
binding adenosine-5'-triphosphate: 11

5tr4A Structure of ubiquitin activating enzyme (uba1) in complex with ubiquitin and tak-243
30% identity, 60% coverage: 9:204/329 of query aligns to 402:606/972 of 5tr4A

query
sites
5tr4A

S

S

R

R

F

Y

D

D

R

N

Q

Q

I

I

A

A

L

V

P

-

E

-

V

F

G

G

A

L

E

D

G

F

Q

Q

A

K

K

K

L

I

A

A

A

N

A

S

H

K

V

V

L

F

V

L

V
|
V

G
|
G

A

S

G
|
G

G
x
A

L

I

G

G

S

C

P

E

V

M

L

L

Q

K

Y

N

L

W

A

A

G

L

A

L

G

G

I

L

G

G

E

S

-

G

-

S

-

D

-

G

-

Y

I

I

T

V

V

V

M
x
T

D
|
D

G
x
M

D
|
D

S

S

V

I

E

E

A

K

T

S

N

N

L

L

P

N

R
|
R

Q
|
Q

P

F

L

L

Y

F

T

R

P

P

A

K

D

D

I

V

G

G

R

K

Y

N

K
|
K

V

S

D

E

A

V

A

A

R

A

E

E

R

A

L

V

C

C

A

A

M

M

A

N

P

P

E

D

L

L

-

K

-

G

-

K

-

I

-

N

-

A

-

K

-

I

-

D

R
|
R

L
x
V

H

G

P

P

H

E

A

T

R

E

D

E

L

I

T

F

P

N

D

D

N

S

V

F

M

W

A

E

A

S

V

-

T

-

P

-

V

L

D

D

L

F

V

V

I

T

D

N

A
|
A

A
x
L

D
|
D

S
x
N

H

V

A
x
D

V
x
A

S

R

Y

T

T
x
Y

L

V

S

D

D

R

C

R

C

C

Q

V

R

F

L

Y

G

R

R

K

D

P

L

L

I

L

S

E

G

S

S

G

A

T

L

L

A

G

Q

T

S

K

G

G

Y

N

V

T

G

Q

V

V

F

I

C

I

G

-

P

P

S

R

L

L

R

T

A

E

V

S

F

Y

P

S

-

S

-

R

D

D

P

P

-

E

G

K

S

S

A

I

A

P

T

L

C
|
C

A
x
T

T

L

S
x
R

G

S

I

F

M

P

G

N

P

K

V

I

V

D

G

H

V

T

I

I

G

-

A

A

W

W

Q

A

A

K

Q

S

L

L

active site: R468 (= R72), D531 (= D129), C587 (= C184), T588 (≠ A185), R590 (≠ S187)
binding [(1~{R},2~{R},3~{S},4~{R})-2,3-bis(oxidanyl)-4-[[2-[3-(trifluoromethylsulfanyl)phenyl]pyrazolo[1,5-a]pyrimidin-7-yl]amino]cyclopentyl]methyl sulfamate: V427 (= V36), G428 (= G37), G430 (= G39), A431 (≠ G40), T456 (≠ M60), D457 (= D61), M458 (≠ G62), D459 (= D63), R468 (= R72), Q469 (= Q73), K481 (= K85), R506 (= R101), V507 (≠ L102), A529 (= A127), L530 (≠ A128), D531 (= D129), N532 (≠ S130), D534 (≠ A132), A535 (≠ V133), Y538 (≠ T136)

Sites not aligning to the query:

active site: 8, 751, 752

7k5jI Structure of an e1-e2-ubiquitin thioester mimetic (see paper)
31% identity, 47% coverage: 9:162/329 of query aligns to 404:566/979 of 7k5jI

query
sites
7k5jI

S

S

R

R

F

Y

D

D

R

N

Q

Q

I

I

A

A

L

V

P

-

E

-

V

F

G

G

A

L

E

D

G

F

Q

Q

A

K

K

K

L

I

A

A

A

N

A

S

H

K

V

V

L

F

V

L

V

V

G

G

A
x
S

G
|
G

G
x
A

L

I

G

G

S

C

P

E

V

M

L

L

Q

K

Y

N

L

W

A

A

G

L

A

L

G

G

I

L

G

G

E

S

-

G

-

S

-

D

-

G

-

Y

I

I

T

V

V

V

M

T

D
|
D

G
x
N

D
|
D

S

S

V

I

E

E

A

K

T

S

N

N

L

L

P

N

R
|
R

Q

Q

P

F

L

L

Y

F

T

R

P

P

A

K

D

D

I

V

G

G

R

K

Y

N

K
|
K

V

S

D

E

A

V

A

A

R

A

E

E

R

A

L

V

C

C

A

A

M

M

A

N

P

P

E

D

L

L

-

K

-

G

-

K

-

I

-

N

-

A

-

K

-

I

-

D

R
x
K

L
x
V

H

G

P

P

H

E

A

T

R

E

D

E

L

I

T

F

P

N

D

D

N

S

V

F

M

W

A

E

A

S

V

-

T

-

P

-

V

L

D

D

L

F

V

V

I

T

D

N

A
|
A

A
x
L

D
|
D

S
x
N

H

V

A

D

V
x
A

S

R

Y

T

T

Y

L

V

S

D

D

R

C

R

C

C

Q

V

R

F

L

Y

G

R

R

K

D

P

L

L

I

L

S

E

G

S

S

G

A

T

L

L

A

G

Q

T

S

K

G

G

Y

N

V

T

G

Q

V

V

active site: R470 (= R72), D533 (= D129)
binding adenosine monophosphate: S431 (≠ A38), G432 (= G39), A433 (≠ G40), D459 (= D61), N460 (≠ G62), D461 (= D63), K483 (= K85), K508 (≠ R101), V509 (≠ L102), A531 (= A127), L532 (≠ A128), D533 (= D129), N534 (≠ S130), A537 (≠ V133)

Sites not aligning to the query:

active site: 10, 581, 582, 584

5l6jA Uba1 in complex with ub-mln7243 covalent adduct (see paper)
30% identity, 52% coverage: 9:178/329 of query aligns to 404:582/1006 of 5l6jA

query
sites
5l6jA

S

S

R

R

F

Y

D

D

R

N

Q

Q

I

I

A

A

L

V

P

-

E

-

V

F

G

G

A

L

E

D

G

F

Q

Q

A

K

K

K

L

I

A

A

A

N

A

S

H

K

V

V

L

F

V

L

V
|
V

G
|
G

A

S

G
|
G

G
x
A

L

I

G

G

S

C

P

E

V

M

L

L

Q

K

Y

N

L

W

A

A

G

L

A

L

G

G

I

L

G

G

E

S

-

G

-

S

-

D

-

G

-

Y

I

I

T

V

V

V

M
x
T

D
|
D

G

N

D
|
D

S

S

V

I

E

E

A

K

T

S

N

N

L

L

P

N

R
|
R

Q
|
Q

P

F

L

L

Y

F

T

R

P

P

A

K

D

D

I

V

G

G

R

K

Y

N

K
|
K

V

S

D

E

A

V

A

A

R

A

E

E

R

A

L

V

C

C

A

A

M

M

A

N

P

P

E

D

L

L

-

K

-

G

-

K

-

I

-

N

-

A

-

K

-

I

-

D

R
x
K

L
x
V

H

G

P
|
P

H

E

A

T

R

E

D

E

L

I

T

F

P

N

D

D

N

S

V

F

M

W

A

E

A

S

V

-

T

-

P

-

V

L

D

D

L

F

V

V

I

T

D

N

A
|
A

A
x
L

D
|
D

S
x
N

H

V

A
x
D

V
x
A

S

R

Y

T

T
x
Y

L

V

S

D

D

R

C

R

C

C

Q

V

R

F

L

Y

G

R

R

K

D

P

L

L

I

L

S

E

G

S

S

G

A

T

L

L

A

G

Q

T

S

K

G

G

Y

N

V

T

G

Q

V

V

F

I

C

I

G

-

P

P

S

R

L

L

R

T

A

E

V

S

F

Y

P

S

-

S

-

R

D

D

P

P

active site: R470 (= R72), D533 (= D129)
binding [(1~{R},2~{R},3~{S},4~{R})-2,3-bis(oxidanyl)-4-[[2-[3-(trifluoromethylsulfanyl)phenyl]pyrazolo[1,5-a]pyrimidin-7-yl]amino]cyclopentyl]methyl sulfamate: V429 (= V36), G430 (= G37), G432 (= G39), A433 (≠ G40), T458 (≠ M60), D459 (= D61), D461 (= D63), R470 (= R72), Q471 (= Q73), K483 (= K85), K508 (≠ R101), V509 (≠ L102), P511 (= P104), A531 (= A127), L532 (≠ A128), D533 (= D129), N534 (≠ S130), D536 (≠ A132), A537 (≠ V133), Y540 (≠ T136)

Sites not aligning to the query:

active site: 10, 589, 590, 592, 770, 771

5l6iA Uba1 in complex with ub-mln4924 covalent adduct (see paper)
30% identity, 52% coverage: 9:178/329 of query aligns to 406:584/1007 of 5l6iA

query
sites
5l6iA

S

S

R

R

F

Y

D

D

R

N

Q

Q

I

I

A

A

L

V

P

-

E

-

V

F

G

G

A

L

E

D

G

F

Q

Q

A

K

K

K

L

I

A

A

A

N

A

S

H

K

V

V

L

F

V

L

V

V

G
|
G

A

S

G
|
G

G
x
A

L

I

G

G

S

C

P

E

V

M

L

L

Q

K

Y

N

L

W

A

A

G

L

A

L

G

G

I

L

G

G

E

S

-

G

-

S

-

D

-

G

-

Y

I

I

T

V

V

V

M

T

D
|
D

G
x
N

D
|
D

S

S

V

I

E

E

A

K

T

S

N

N

L

L

P

N

R
|
R

Q
|
Q

P

F

L

L

Y

F

T

R

P

P

A

K

D

D

I

V

G

G

R

K

Y

N

K
|
K

V

S

D

E

A

V

A

A

R

A

E

E

R

A

L

V

C

C

A

A

M

M

A

N

P

P

E

D

L

L

-

K

-

G

-

K

-

I

-

N

-

A

-

K

-

I

-

D

R
x
K

L
x
V

H
x
G

P

P

H

E

A

T

R

E

D

E

L

I

T

F

P

N

D

D

N

S

V

F

M

W

A

E

A

S

V

-

T

-

P

-

V

L

D

D

L

F

V

V

I

T

D

N

A
|
A

A
x
L

D
|
D

S
x
N

H

V

A
x
D

V
x
A

S

R

Y

T

T
x
Y

L

V

S

D

D

R

C

R

C

C

Q

V

R

F

L

Y

G

R

R

K

D

P

L

L

I

L

S

E

G

S

S

G

A

T

L

L

A

G

Q

T

S

K

G

G

Y

N

V

T

G

Q

V

V

F

I

C

I

G

-

P

P

S

R

L

L

R

T

A

E

V

S

F

Y

P

S

-

S

-

R

D

D

P

P

active site: R472 (= R72), D535 (= D129)
binding [(1S,2S,4R)-4-{4-[(1S)-2,3-dihydro-1H-inden-1-ylamino]-7H-pyrrolo[2,3-d]pyrimidin-7-yl}-2-hydroxycyclopentyl]methyl sulfamate: G432 (= G37), G434 (= G39), A435 (≠ G40), D461 (= D61), N462 (≠ G62), D463 (= D63), R472 (= R72), Q473 (= Q73), K485 (= K85), K510 (≠ R101), V511 (≠ L102), G512 (≠ H103), A533 (= A127), L534 (≠ A128), D535 (= D129), N536 (≠ S130), D538 (≠ A132), A539 (≠ V133), Y542 (≠ T136)

Sites not aligning to the query:

active site: 12, 591, 592, 594, 772, 773

Query Sequence

>GFF891 FitnessBrowser__Phaeo:GFF891
MDQGLRLMSRFDRQIALPEVGAEGQAKLAAAHVLVVGAGGLGSPVLQYLAGAGIGEITVM
DGDSVEATNLPRQPLYTPADIGRYKVDAARERLCAMAPELRLHPHARDLTPDNVMAAVTP
VDLVIDAADSHAVSYTLSDCCQRLGRDLISGSALAQSGYVGVFCGGGPSLRAVFPDPPGS
AATCATSGIMGPVVGVIGAWQAQLALKLLLNHQPTPRGRIFSVDFAGLQMGGFDFSTARE
PARPLPFIGLNAITDEDYVVELRPEQEAPTPAIRTAVRILPEALQARDLPSDRRVVLACH
SGLRAWRTAAEIAPDFAGELVLLAAGQPR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory