SitesBLAST
Comparing GFF900 FitnessBrowser__Marino:GFF900 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
1xg5C Structure of human putative dehydrogenase mgc4172 in complex with nadp
35% identity, 98% coverage: 2:220/224 of query aligns to 14:255/257 of 1xg5C
- active site: S150 (= S133), Y165 (= Y145), K169 (= K149)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G17 (= G5), S19 (= S7), G21 (= G9), I22 (= I10), A41 (= A29), R42 (= R30), T43 (≠ S31), D69 (= D54), L70 (≠ V55), N96 (= N81), G98 (= G83), I148 (≠ T131), S150 (= S133), Y165 (= Y145), K169 (= K149), G198 (= G176), V200 (= V178), T202 (= T180), F204 (= F182), K207 (vs. gap)
A0A1U8QWA2 Glycine betaine reductase ATRR; Nonribosomal peptide synthetase-like protein ATRR; EC 1.2.1.-; EC 1.1.1.- from Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) (see paper)
35% identity, 97% coverage: 2:219/224 of query aligns to 1033:1265/1270 of A0A1U8QWA2
- G1036 (= G5) mutation to A: Compromises binding of the cosubstrate NADPH to aldehyde reductase domain R2. Decreases the aldehyde reductase activity by 4,000-fold; when associated with F-1178.
- Y1178 (= Y145) mutation to F: Does not substantially affect carboxylic acid reductase activity but results to a 150-fold loss of aldehyde reductase activity and the accumulation of glycine betaine aldehyde intermediate. Further decreases the aldehyde reductase activity by 4,000-fold; when associated with F-1178.
Sites not aligning to the query:
- 14:418 Adenylation (A) domain
- 643:937 Carboxylic acid reductase domain R1
- 812 Y→F: Abolishes overall carboxylic acid reductase activity but does nor affect aldehyde reductase activity.
- 1026:1256 Aldehyde reductase domain R2
3rkrA Crystal structure of a metagenomic short-chain oxidoreductase (sdr) in complex with NADP (see paper)
34% identity, 99% coverage: 2:222/224 of query aligns to 9:221/221 of 3rkrA
- active site: G16 (= G9), S142 (= S133), Y155 (= Y145), K159 (= K149)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G5), R15 (≠ S8), A36 (= A29), R37 (= R30), D38 (≠ S31), C61 (= C53), D62 (= D54), L63 (≠ V55), A90 (= A82), G91 (= G83), V92 (≠ R84), G93 (= G85)
2japA Clavulanic acid dehydrogenase: structural and biochemical analysis of the final step in the biosynthesis of the beta- lactamase inhibitor clavulanic acid (see paper)
37% identity, 99% coverage: 2:223/224 of query aligns to 9:245/245 of 2japA
- active site: S140 (= S133), Y153 (= Y145), K157 (= K149), A198 (vs. gap)
- binding (2r,3z,5r)-3-(2-hydroxyethylidene)-7-oxo-4-oxa-1-azabicyclo[3.2.0]heptane-2-carboxylic acid: M93 (≠ G86), S140 (= S133), A142 (= A135), Y153 (= Y145), T185 (≠ M177), Y203 (≠ F183), R206 (= R186)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G12 (= G5), S14 (= S7), S15 (= S8), G16 (= G9), I17 (= I10), A36 (= A29), R37 (= R30), R38 (≠ S31), L61 (≠ C53), D62 (= D54), V63 (= V55), N89 (= N81), A90 (= A82), G91 (= G83), T112 (= T105), M138 (≠ T131), S139 (≠ G132), S140 (= S133), Y153 (= Y145), K157 (= K149), P183 (= P175), T185 (≠ M177), T186 (≠ V178), T188 (= T180), E189 (≠ P181), L190 (≠ F182)
2jahC Biochemical and structural analysis of the clavulanic acid dehydeogenase (cad) from streptomyces clavuligerus (see paper)
37% identity, 99% coverage: 2:223/224 of query aligns to 10:246/246 of 2jahC
- active site: S141 (= S133), Y154 (= Y145), K158 (= K149), A199 (vs. gap)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G13 (= G5), S15 (= S7), S16 (= S8), G17 (= G9), I18 (= I10), A37 (= A29), R38 (= R30), R39 (≠ S31), D63 (= D54), V64 (= V55), N90 (= N81), A91 (= A82), G92 (= G83), T113 (= T105), M139 (≠ T131), S141 (= S133), Y154 (= Y145), K158 (= K149), P184 (= P175), G185 (= G176), T186 (≠ M177), T187 (≠ V178), T189 (= T180), E190 (≠ P181), L191 (≠ F182)
2ehdB Crystal structure analysis of oxidoreductase
35% identity, 98% coverage: 1:220/224 of query aligns to 7:213/213 of 2ehdB
3l77A X-ray structure alcohol dehydrogenase from archaeon thermococcus sibiricus complexed with 5-hydroxy-NADP
36% identity, 97% coverage: 2:218/224 of query aligns to 6:227/235 of 3l77A
- active site: D139 (≠ A134), Y151 (= Y145), K155 (= K149), P194 (vs. gap)
- binding 5-hydroxy-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE: G9 (= G5), S11 (= S7), R12 (≠ S8), G13 (= G9), I14 (= I10), R34 (= R30), S35 (= S31), L59 (≠ C53), D60 (= D54), V61 (= V55), N87 (= N81), A88 (= A82), G89 (= G83), T136 (= T131), T137 (≠ G132), S138 (= S133), Y151 (= Y145), K155 (= K149), P179 (= P175), G180 (= G176), A181 (≠ M177), V182 (= V178), T184 (= T180), Y185 (≠ P181), F186 (= F182)
6ixjA The crystal structure of sulfoacetaldehyde reductase from klebsiella oxytoca (see paper)
32% identity, 98% coverage: 1:219/224 of query aligns to 4:238/251 of 6ixjA
- binding 2-hydroxyethylsulfonic acid: S138 (= S133), Y145 (≠ I140), Y151 (= Y145)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G8 (= G5), T10 (≠ S7), S11 (= S8), G12 (= G9), F13 (≠ I10), R33 (= R30), R34 (≠ S31), D57 (= D54), V58 (= V55), N84 (= N81), A85 (= A82), G86 (= G83), T108 (= T105), I136 (≠ T131), S138 (= S133), Y151 (= Y145), K155 (= K149), P181 (= P175), G182 (= G176), A184 (≠ V178), T186 (= T180), E187 (≠ P181), F188 (= F182)
3asvA The closed form of serine dehydrogenase complexed with NADP+ (see paper)
31% identity, 99% coverage: 1:222/224 of query aligns to 3:238/248 of 3asvA
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G7 (= G5), T9 (≠ S7), G11 (= G9), F12 (≠ I10), R32 (= R30), R33 (≠ S31), D54 (= D54), V55 (= V55), N81 (= N81), G83 (= G83), I132 (≠ T131), S134 (= S133), Y147 (= Y145), K151 (= K149), G178 (= G176), V180 (= V178), T183 (= T180), E184 (≠ P181), F185 (= F182)
- binding phosphate ion: S134 (= S133), Y147 (= Y145), G178 (= G176), F185 (= F182)
3p19A Improved NADPH-dependent blue fluorescent protein (see paper)
31% identity, 99% coverage: 1:222/224 of query aligns to 5:237/239 of 3p19A
- active site: S132 (= S133), Y145 (= Y145), K149 (= K149)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G9 (= G5), S11 (= S7), S12 (= S8), G13 (= G9), I14 (= I10), A33 (= A29), R34 (= R30), R35 (≠ S31), D53 (= D54), V54 (= V55), N80 (= N81), A81 (= A82), G82 (= G83), I130 (≠ T131), S132 (= S133), Y145 (= Y145), K149 (= K149), P175 (= P175), A177 (≠ M177), V178 (= V178), T180 (= T180), E181 (≠ P181), L182 (≠ F182)
Q9P7B4 NADP-dependent 3-hydroxy acid dehydrogenase; L-allo-threonine dehydrogenase; EC 1.1.1.381 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
32% identity, 98% coverage: 1:220/224 of query aligns to 9:246/259 of Q9P7B4
- S42 (≠ D33) modified: Phosphoserine
- T43 (≠ K34) modified: Phosphothreonine
3rkuA Substrate fingerprint and the structure of NADP+ dependent serine dehydrogenase from saccharomyces cerevisiae complexed with NADP+
31% identity, 98% coverage: 1:220/224 of query aligns to 17:257/268 of 3rkuA
- active site: A107 (≠ G85), N128 (= N106), S156 (= S133), Y169 (= Y145), K173 (= K149), N214 (vs. gap)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G21 (= G5), S23 (= S7), G25 (= G9), I26 (= I10), R49 (= R30), R50 (≠ S31), D76 (= D54), I77 (≠ V55), N103 (= N81), A104 (= A82), G105 (= G83), K106 (≠ R84), S156 (= S133), Y169 (= Y145), K173 (= K149), P199 (= P175), G200 (= G176), V202 (= V178), T204 (= T180), E205 (≠ P181), F206 (= F182)
Q05016 NADP-dependent 3-hydroxy acid dehydrogenase; L-allo-threonine dehydrogenase; EC 1.1.1.-; EC 1.1.1.381 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
31% identity, 98% coverage: 1:220/224 of query aligns to 16:256/267 of Q05016
- N102 (= N81) binding
- Y168 (= Y145) binding
- K172 (= K149) binding
3iahA Crystal structure of short chain dehydrogenase (ycik) from salmonella enterica subsp. Enterica serovar typhimurium str. Lt2 in complex with NADP and acetate.
30% identity, 88% coverage: 1:196/224 of query aligns to 15:221/253 of 3iahA
- active site: S152 (= S133), Y165 (= Y145), K169 (= K149)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G19 (= G5), S21 (= S7), I24 (= I10), R44 (= R30), K48 (= K34), D70 (≠ Q52), L71 (≠ C53), N99 (= N81), G101 (= G83), T150 (= T131), Y165 (= Y145), K169 (= K149), P195 (= P175), G196 (= G176), G197 (vs. gap), T198 (vs. gap), T200 (vs. gap), M202 (= M177), R203 (≠ V178)
2rh4B Actinorhodin ketoreductase, actkr, with NADPH and inhibitor emodin (see paper)
34% identity, 81% coverage: 2:182/224 of query aligns to 17:201/268 of 2rh4B
- active site: G24 (= G9), N121 (= N106), S151 (= S133), Y164 (= Y145), K168 (= K149)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G20 (= G5), T22 (≠ S7), S23 (= S8), I25 (= I10), A44 (= A29), R45 (= R30), G46 (≠ S31), C69 (= C53), D70 (= D54), V71 (= V55), N97 (= N81), S151 (= S133), Y164 (= Y145), K168 (= K149), G195 (= G176), V197 (= V178), T199 (= T180), M201 (≠ F182)
Sites not aligning to the query:
1w4zA Structure of actinorhodin polyketide (actiii) reductase (see paper)
34% identity, 81% coverage: 2:182/224 of query aligns to 8:192/259 of 1w4zA
- active site: G15 (= G9), N112 (= N106), S142 (= S133), Y155 (= Y145), K159 (= K149)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G11 (= G5), T13 (≠ S7), S14 (= S8), G15 (= G9), I16 (= I10), R36 (= R30), G37 (≠ S31), D61 (= D54), V62 (= V55), N88 (= N81), G90 (= G83), S142 (= S133), Y155 (= Y145), K159 (= K149), P185 (= P175), G186 (= G176), V188 (= V178), T190 (= T180)
Sites not aligning to the query:
1xr3A Actinorhodin polyketide ketoreductase with NADP and the inhibitor isoniazid bound (see paper)
34% identity, 81% coverage: 2:182/224 of query aligns to 5:189/256 of 1xr3A
- active site: G12 (= G9), N109 (= N106), S139 (= S133), Y152 (= Y145), K156 (= K149)
- binding 4-(diazenylcarbonyl)pyridine: T140 (≠ A134), G141 (≠ A135), V146 (≠ T139)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G5), T10 (≠ S7), S11 (= S8), G12 (= G9), I13 (= I10), A32 (= A29), R33 (= R30), G34 (≠ S31), C57 (= C53), D58 (= D54), V59 (= V55), N85 (= N81), A86 (= A82), G87 (= G83), S139 (= S133), Y152 (= Y145), K156 (= K149), G183 (= G176), V185 (= V178), T187 (= T180), P188 (= P181)
Sites not aligning to the query:
2rh4A Actinorhodin ketoreductase, actkr, with NADPH and inhibitor emodin (see paper)
34% identity, 81% coverage: 2:182/224 of query aligns to 6:190/257 of 2rh4A
- active site: G13 (= G9), N110 (= N106), S140 (= S133), Y153 (= Y145), K157 (= K149)
- binding 3-methyl-1,6,8-trihydroxyanthraquinone: T141 (≠ A134), Q145 (vs. gap), V147 (≠ T139), Y153 (= Y145), F185 (≠ M177)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G9 (= G5), T11 (≠ S7), S12 (= S8), G13 (= G9), I14 (= I10), A33 (= A29), R34 (= R30), G35 (≠ S31), C58 (= C53), D59 (= D54), V60 (= V55), N86 (= N81), G88 (= G83), S140 (= S133), Y153 (= Y145), K157 (= K149), P183 (= P175), G184 (= G176), V186 (= V178), T188 (= T180), M190 (≠ F182)
Sites not aligning to the query:
P16544 Putative ketoacyl reductase; EC 1.3.1.- from Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) (see 2 papers)
34% identity, 81% coverage: 2:182/224 of query aligns to 10:194/261 of P16544
- 11:39 (vs. 3:31, 52% identical) binding
- D63 (= D54) binding
- K161 (= K149) binding
6gtuA 17beta-hydroxysteroid dehydrogenase 14 variant t205 in complex with fragment j6
31% identity, 86% coverage: 16:207/224 of query aligns to 24:211/268 of 6gtuA
- active site: S138 (vs. gap), Y151 (= Y145), K155 (= K149)
- binding N-(1,3-benzodioxol-5-ylmethyl)cyclopentanamine: N183 (≠ M177)
- binding nicotinamide-adenine-dinucleotide: D37 (≠ A29), K38 (≠ R30), D59 (= D54), N86 (= N81), A87 (= A82), G88 (= G83), L110 (≠ T105), S138 (vs. gap), Y151 (= Y145), K155 (= K149), G182 (= G176), I184 (≠ V178), T186 (= T180), L188 (≠ F182)
Sites not aligning to the query:
Query Sequence
>GFF900 FitnessBrowser__Marino:GFF900
MLITGASSGIGAETARAAAKQGYRLVLAARSEDKLKGLQQELGGEEKVLTVQCDVQSGDD
QKNMVDQALKTFGRIDAVFANAGRGGEPGGFSGADPDVWKDMILTNIYGVGLTLQHCMPA
LKESKGHLLLTGSAAGRATIPGSMYSATKWAVTAIGYGVREELRGSGIRVTLIEPGMVDT
PFFDERPGHALKPEDIANAVMYAVAQPAHVDVNEILVRPTPKVE
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory