SitesBLAST
Comparing GFF928 FitnessBrowser__Marino:GFF928 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
1rx0A Crystal structure of isobutyryl-coa dehydrogenase complexed with substrate/ligand. (see paper)
54% identity, 97% coverage: 5:381/388 of query aligns to 10:384/384 of 1rx0A
- active site: L129 (= L124), T130 (= T125), G245 (= G242), E367 (= E364), R379 (= R376)
- binding flavin-adenine dinucleotide: Y127 (= Y122), L129 (= L124), T130 (= T125), G135 (= G130), S136 (= S131), F160 (= F155), I161 (= I156), S162 (= S157), W207 (= W204), R271 (= R268), F274 (= F271), L278 (= L275), N281 (≠ F278), L284 (= L281), Q340 (= Q337), M341 (≠ L338), G343 (= G340), G344 (= G341), Y345 (= Y342), L366 (= L363), S369 (≠ T366), E371 (= E368)
1rx0C Crystal structure of isobutyryl-coa dehydrogenase complexed with substrate/ligand. (see paper)
54% identity, 97% coverage: 5:381/388 of query aligns to 9:383/383 of 1rx0C
- active site: L128 (= L124), T129 (= T125), G244 (= G242), E366 (= E364), R378 (= R376)
- binding methacrylyl-coenzyme a: I93 (= I89), Y126 (= Y122), S135 (= S131), V238 (≠ M236), L241 (= L239), N242 (≠ D240), R245 (= R243), V316 (≠ T314), E366 (= E364), G367 (= G365), L375 (≠ I373), R378 (= R376)
- binding flavin-adenine dinucleotide: Y126 (= Y122), L128 (= L124), T129 (= T125), G134 (= G130), S135 (= S131), F159 (= F155), I160 (= I156), S161 (= S157), R270 (= R268), F273 (= F271), N280 (≠ F278), L283 (= L281), Q339 (= Q337), M340 (≠ L338), G342 (= G340), G343 (= G341), Y344 (= Y342), L365 (= L363), S368 (≠ T366), E370 (= E368)
Q9UKU7 Isobutyryl-CoA dehydrogenase, mitochondrial; IBDH; Activator-recruited cofactor 42 kDa component; ARC42; Acyl-CoA dehydrogenase family member 8; ACAD-8; EC 1.3.8.5 from Homo sapiens (Human) (see 3 papers)
54% identity, 97% coverage: 5:381/388 of query aligns to 41:415/415 of Q9UKU7
- G137 (≠ A101) to R: in IBDD; loss of protein solubility; complete loss of isobutyryl-CoA dehydrogenase activity; dbSNP:rs371449613
- 158:167 (vs. 122:131, 90% identical) binding in other chain
- S167 (= S131) binding
- FIS 191:193 (= FIS 155:157) binding in other chain
- NGGR 274:277 (≠ DGGR 240:243) binding
- R302 (= R268) binding ; to Q: in IBDD; no effect on localization to the mitochondrion; complete loss of isobutyryl-CoA dehydrogenase activity; loss of protein expression in patient cells; dbSNP:rs121908422
- NQ 312:313 (≠ FQ 278:279) binding
- A320 (= A286) to T: in IBDD; decreased isobutyryl-CoA dehydrogenase activity; less than 20% of wild-type; dbSNP:rs200620279
- QMHGG 371:375 (≠ QLHGG 337:341) binding
- SNE 400:402 (≠ TNE 366:368) binding in other chain
- R410 (= R376) binding
5ol2F The electron transferring flavoprotein/butyryl-coa dehydrogenase complex from clostridium difficile (see paper)
42% identity, 98% coverage: 1:379/388 of query aligns to 1:377/378 of 5ol2F
- active site: L124 (= L124), T125 (= T125), G241 (= G242), G374 (≠ R376)
- binding calcium ion: E29 (≠ K30), E33 (= E34), R35 (≠ I36)
- binding coenzyme a persulfide: L238 (= L239), R242 (= R243), E362 (= E364), G363 (= G365)
- binding flavin-adenine dinucleotide: F122 (≠ Y122), L124 (= L124), T125 (= T125), P127 (= P127), T131 (≠ S131), F155 (= F155), I156 (= I156), T157 (≠ S157), E198 (= E199), R267 (= R268), F270 (= F271), L274 (= L275), F277 (= F278), Q335 (= Q337), L336 (= L338), G338 (= G340), G339 (= G341), Y361 (≠ L363), T364 (= T366), E366 (= E368)
7w0jE Acyl-coa dehydrogenase, tfu_1647
43% identity, 97% coverage: 2:379/388 of query aligns to 1:381/382 of 7w0jE
- binding [[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2R,3S,4S)-5-azanyl-2,3,4-tris(oxidanyl)pentyl] hydrogen phosphate: S127 (≠ T125), W157 (≠ F155), R270 (= R268), Q272 (= Q270), F273 (= F271), I277 (≠ L275), F280 (= F278), I283 (≠ L281), Q339 (= Q337), L340 (= L338), G343 (= G341), Y365 (≠ L363), E366 (= E364), T368 (= T366), Q370 (≠ E368), I371 (= I369)
8i4rA Crystal structure of acyl-coa dehydrogenase complexed with acetyl-coa from thermobifida fusca
43% identity, 97% coverage: 3:379/388 of query aligns to 4:380/381 of 8i4rA
- binding acetyl coenzyme *a: S132 (= S131), T134 (≠ A133), R180 (≠ K179), R234 (≠ A233), L237 (≠ M236), R238 (≠ K237), L240 (= L239), D241 (= D240), R244 (= R243), E365 (= E364), G366 (= G365), R377 (= R376)
- binding flavin-adenine dinucleotide: Y123 (= Y122), L125 (= L124), S126 (≠ T125), G131 (= G130), S132 (= S131), W156 (≠ F155), I157 (= I156), T158 (≠ S157), I360 (≠ V359), T367 (= T366), Q369 (≠ E368)
8i4pA Crystal structure of acyl-coa dehydrogenase from thermobifida fusca
43% identity, 97% coverage: 3:379/388 of query aligns to 4:380/381 of 8i4pA
- binding flavin-adenine dinucleotide: Y123 (= Y122), L125 (= L124), S126 (≠ T125), G131 (= G130), S132 (= S131), W156 (≠ F155), I157 (= I156), T158 (≠ S157), I360 (≠ V359), Y364 (≠ L363), T367 (= T366), Q369 (≠ E368)
4l1fA Electron transferring flavoprotein of acidaminococcus fermentans: towards a mechanism of flavin-based electron bifurcation (see paper)
42% identity, 98% coverage: 1:381/388 of query aligns to 1:380/380 of 4l1fA
- active site: L125 (= L124), T126 (= T125), G242 (= G242), E363 (= E364), R375 (= R376)
- binding coenzyme a persulfide: T132 (≠ S131), H179 (≠ K179), F232 (= F232), M236 (= M236), E237 (≠ K237), L239 (= L239), D240 (= D240), R243 (= R243), Y362 (≠ L363), E363 (= E364), G364 (= G365), R375 (= R376)
- binding flavin-adenine dinucleotide: F123 (≠ Y122), L125 (= L124), T126 (= T125), G131 (= G130), T132 (≠ S131), F156 (= F155), I157 (= I156), T158 (≠ S157), R268 (= R268), Q270 (= Q270), F271 (= F271), I275 (≠ L275), F278 (= F278), L281 (= L281), Q336 (= Q337), I337 (≠ L338), G340 (= G341), I358 (≠ V359), Y362 (≠ L363), T365 (= T366), Q367 (≠ E368)
- binding 1,3-propandiol: L5 (= L5), Q10 (≠ L10)
5lnxD Crystal structure of mmgc, an acyl-coa dehydrogenase from bacillus subtilis.
41% identity, 96% coverage: 6:379/388 of query aligns to 3:373/374 of 5lnxD
- active site: L122 (= L124), T123 (= T125), G239 (= G242), E358 (= E364), K370 (≠ R376)
- binding flavin-adenine dinucleotide: L122 (= L124), T123 (= T125), G128 (= G130), S129 (= S131), F153 (= F155), T155 (≠ S157), R265 (= R268), Q267 (= Q270), F268 (= F271), I272 (≠ L275), N275 (≠ F278), I278 (≠ L281), Q331 (= Q337), I332 (≠ L338), G335 (= G341), Y357 (≠ L363), T360 (= T366), E362 (= E368)
1ukwB Crystal structure of medium-chain acyl-coa dehydrogenase from thermus thermophilus hb8
41% identity, 98% coverage: 1:379/388 of query aligns to 1:377/379 of 1ukwB
- active site: L124 (= L124), S125 (≠ T125), T241 (≠ G242), E362 (= E364), R374 (= R376)
- binding cobalt (ii) ion: D145 (= D145), H146 (≠ S146)
- binding flavin-adenine dinucleotide: F122 (≠ Y122), L124 (= L124), S125 (≠ T125), G130 (= G130), S131 (= S131), W155 (≠ F155), S157 (= S157), K200 (= K201), L357 (≠ V359), Y361 (≠ L363), E362 (= E364), T364 (= T366), E366 (= E368), L370 (= L372)
1ukwA Crystal structure of medium-chain acyl-coa dehydrogenase from thermus thermophilus hb8
41% identity, 98% coverage: 1:379/388 of query aligns to 1:377/379 of 1ukwA
- active site: L124 (= L124), S125 (≠ T125), T241 (≠ G242), E362 (= E364), R374 (= R376)
- binding flavin-adenine dinucleotide: F122 (≠ Y122), L124 (= L124), S125 (≠ T125), G130 (= G130), S131 (= S131), W155 (≠ F155), S157 (= S157), L357 (≠ V359), Y361 (≠ L363), E362 (= E364), T364 (= T366), E366 (= E368), L370 (= L372)
4n5fA Crystal structure of a putative acyl-coa dehydrogenase with bound fadh2 from burkholderia cenocepacia j2315
42% identity, 97% coverage: 1:378/388 of query aligns to 2:378/378 of 4n5fA
- active site: L126 (= L124), T127 (= T125), G243 (= G242), E364 (= E364), R376 (= R376)
- binding dihydroflavine-adenine dinucleotide: L126 (= L124), T127 (= T125), G132 (= G130), S133 (= S131), F157 (= F155), T159 (≠ S157), T210 (= T209), Y363 (≠ L363), T366 (= T366), E368 (= E368), M372 (≠ L372)
3pfdC Crystal structure of an acyl-coa dehydrogenase from mycobacterium thermoresistibile bound to reduced flavin adenine dinucleotide solved by combined iodide ion sad mr (see paper)
41% identity, 96% coverage: 9:379/388 of query aligns to 1:368/369 of 3pfdC
- active site: L116 (= L124), S117 (≠ T125), T233 (≠ G242), E353 (= E364), R365 (= R376)
- binding dihydroflavine-adenine dinucleotide: Y114 (= Y122), L116 (= L124), S117 (≠ T125), G122 (= G130), S123 (= S131), W147 (≠ F155), I148 (= I156), T149 (≠ S157), R259 (= R268), F262 (= F271), V266 (≠ L275), N269 (≠ F278), Q326 (= Q337), L327 (= L338), G330 (= G341), I348 (≠ V359), Y352 (≠ L363), T355 (= T366), Q357 (≠ E368)
1jqiA Crystal structure of rat short chain acyl-coa dehydrogenase complexed with acetoacetyl-coa (see paper)
41% identity, 97% coverage: 5:379/388 of query aligns to 7:380/384 of 1jqiA
- active site: G377 (≠ R376)
- binding acetoacetyl-coenzyme a: L95 (≠ M92), F125 (≠ Y122), S134 (= S131), F234 (= F232), M238 (= M236), Q239 (≠ K237), L241 (= L239), D242 (= D240), R245 (= R243), Y364 (≠ L363), E365 (= E364), G366 (= G365)
- binding flavin-adenine dinucleotide: F125 (≠ Y122), L127 (= L124), S128 (≠ T125), G133 (= G130), S134 (= S131), W158 (≠ F155), T160 (≠ S157), R270 (= R268), F273 (= F271), L280 (≠ F278), Q338 (= Q337), I339 (≠ L338), G342 (= G341), I360 (≠ V359), T367 (= T366), E369 (= E368), I370 (= I369)
P15651 Short-chain specific acyl-CoA dehydrogenase, mitochondrial; SCAD; Butyryl-CoA dehydrogenase; EC 1.3.8.1 from Rattus norvegicus (Rat) (see 2 papers)
41% identity, 97% coverage: 5:379/388 of query aligns to 34:407/412 of P15651
Sites not aligning to the query:
- 1:24 modified: transit peptide, Mitochondrion
4m9aB Crystal structure of acyl-coa dehydrogenase from burkholderia thailandensis e264
42% identity, 96% coverage: 6:378/388 of query aligns to 5:376/376 of 4m9aB
- active site: L124 (= L124), T125 (= T125), G241 (= G242), E362 (= E364), R374 (= R376)
- binding dihydroflavine-adenine dinucleotide: F122 (≠ Y122), T125 (= T125), G130 (= G130), S131 (= S131), F155 (= F155), T157 (≠ S157), T208 (= T209), Y361 (≠ L363), T364 (= T366), E366 (= E368), M370 (≠ L372)
2dvlA Crystal structure of project tt0160 from thermus thermophilus hb8
43% identity, 96% coverage: 5:378/388 of query aligns to 1:368/370 of 2dvlA
- active site: L121 (= L124), T122 (= T125), G233 (= G242), E354 (= E364), R366 (= R376)
- binding flavin-adenine dinucleotide: L121 (= L124), T122 (= T125), G127 (= G130), S128 (= S131), W152 (≠ F155), I153 (= I156), T154 (≠ S157), T356 (= T366), E358 (= E368)
1bucA Three-dimensional structure of butyryl-coa dehydrogenase from megasphaera elsdenii (see paper)
40% identity, 98% coverage: 1:379/388 of query aligns to 1:382/383 of 1bucA
- active site: L128 (= L124), T129 (= T125), G246 (= G242), E367 (= E364), G379 (≠ R376)
- binding acetoacetyl-coenzyme a: L96 (≠ I89), F126 (≠ Y122), G134 (= G130), T135 (≠ S131), T162 (≠ S157), N182 (≠ Y178), H183 (≠ K179), F236 (= F232), M240 (= M236), M241 (≠ K237), L243 (= L239), D244 (= D240), T317 (= T314), Y366 (≠ L363), E367 (= E364), G368 (= G365)
- binding flavin-adenine dinucleotide: F126 (≠ Y122), L128 (= L124), T129 (= T125), G134 (= G130), T135 (≠ S131), F160 (= F155), T162 (≠ S157), Y366 (≠ L363), T369 (= T366), E371 (= E368), M375 (≠ L372)
Q06319 Acyl-CoA dehydrogenase, short-chain specific; Butyryl-CoA dehydrogenase; BCAD; SCAD; EC 1.3.8.1 from Megasphaera elsdenii (see paper)
40% identity, 98% coverage: 1:379/388 of query aligns to 1:382/383 of Q06319
- E367 (= E364) active site, Proton acceptor; mutation to Q: Loss of activity.
P16219 Short-chain specific acyl-CoA dehydrogenase, mitochondrial; SCAD; Butyryl-CoA dehydrogenase; EC 1.3.8.1 from Homo sapiens (Human) (see 3 papers)
40% identity, 97% coverage: 5:379/388 of query aligns to 34:407/412 of P16219
- G90 (= G61) to S: in ACADSD; loss of acyl-CoA dehydrogenase activity; dbSNP:rs121908005
- E104 (= E75) natural variant: Missing (in ACADSD; loss of acyl-CoA dehydrogenase activity; dbSNP:rs387906308)
- 152:161 (vs. 122:131, 60% identical) binding in other chain
- R171 (≠ V141) to W: 69% of wild-type acyl-CoA dehydrogenase activity; confers susceptibility to ethylmalonicaciduria; dbSNP:rs1800556
- WIT 185:187 (≠ FIS 155:157) binding in other chain
- A192 (≠ T162) to V: in ACADSD; loss of acyl-CoA dehydrogenase activity; dbSNP:rs28940874
- G209 (= G180) to S: 86% of wild-type acyl-CoA dehydrogenase activity; confers susceptibility to ethylmalonicaciduria; dbSNP:rs1799958
- R297 (= R268) binding
- Q308 (= Q279) binding in other chain
- R325 (= R296) to W: in ACADSD; loss of acyl-CoA dehydrogenase activity; dbSNP:rs121908006
- S353 (≠ T325) to L: in ACADSD; loss of acyl-CoA dehydrogenase activity; dbSNP:rs28941773
- QILGG 365:369 (≠ QLHGG 337:341) binding
- R380 (= R352) to W: in ACADSD; loss of acyl-CoA dehydrogenase activity; dbSNP:rs28940875
- TSE 394:396 (≠ TNE 366:368) binding in other chain
Sites not aligning to the query:
- 1:24 modified: transit peptide, Mitochondrion
Query Sequence
>GFF928 FitnessBrowser__Marino:GFF928
MDFNLTEDQLAFREAARAFAEKSMAPHAAKWDNEHIFPVDMMKEAGEMGFMGMYTPEALG
GMGLSRLDTSVIVEELAAACPSTAAFITIHNMATWMVASFASDDLKQEIVPKLASGEWLA
SYCLTEPGAGSDAASLRTKAVRDGDSYVINGSKVFISGAGATDILVLMARTGAPDSGYKG
ISTFVIPADADGISYGKNEEKMGWHSQPTRMISLENVRIPASNRVGDEGDGFAIAMKGLD
GGRLNIATCSLGGAQAALLRARNYMHEREQFGKPLAAFQALQFKLADMATNLVAARQMVR
LGAFKLDNADPEATLHCAMAKRFATDACFEVVNEALQLHGGYGYIREYPLERYLRDLRVH
QILEGTNEIMRLIVARRLLDDGVAEAIQ
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory